Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1613,6010GT24.8% 425.0 / 27.2 286intergenic (+462/+561)garK/yhaCglycerate 2‑kinase/pentapeptide repeats‑related protein
Reads supporting (aligned to +/- strand):  ref base G (64/115);  new base T (6/53);  total (81/205)
Fisher's exact test for biased strand distribution p-value = 1.20e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 290 total aligned reads displayed.

GTACTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCA  >  DH1/613451‑613743
                                                                                                                                                       |                                                                                                                                              
  aCTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGat                                                                                                                                              <  7:285033/151‑1 (MQ=255)
  aCTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGat                                                                                                                                              <  4:1403286/151‑1 (MQ=255)
  aCTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGat                                                                                                                                              <  7:927631/151‑1 (MQ=255)
  aCTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGat                                                                                                                                              <  5:731962/151‑1 (MQ=255)
   cTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACACAAAACCCAAAATACCTCACTATCACCTGATATACGTAAAAACCAA‑CTTAGGAAAGATTTTTCTTTTGGAGGGTCATAAACat                                                                                                                                              >  8:1043345/1‑150 (MQ=255)
     gAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGatga                                                                                                                                            <  2:790587/150‑1 (MQ=255)
       ggcgggtttttgtgtttGAGCGAGGGAGAGTTTGATGGCCTAGAGCCCAGACTCTACACCACAGAACGCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGatgaatg                                                                                                                                         <  5:1533948/136‑1 (MQ=2)
          cGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGGCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACt                                                                                                                                      <  3:380752/151‑1 (MQ=255)
          cGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACt                                                                                                                                      <  7:817373/151‑1 (MQ=255)
           gggTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTg                                                                                                                                     <  7:783511/151‑1 (MQ=255)
           gggTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTg                                                                                                                                     <  1:1496597/151‑1 (MQ=255)
           gggTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTCCTTTTGGAGGGGCAGAGAGATGAATTAcgg                                                                                                                                     >  1:1375380/1‑149 (MQ=255)
           gggTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTACTTATGTAGGTTCAGAAAGATGCTTGACTg                                                                                                                                     >  3:1837039/1‑151 (MQ=25)
               aGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTcc                                                                                                                                  <  3:1037767/150‑1 (MQ=255)
               aGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCa                                                                                                                                 <  3:1110239/151‑1 (MQ=255)
               aGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCa                                                                                                                                 <  2:171085/151‑1 (MQ=255)
                ggCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCac                                                                                                                                <  3:1796029/151‑1 (MQ=255)
                ggCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCac                                                                                                                                <  4:1563172/151‑1 (MQ=255)
                ggCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCac                                                                                                                                <  1:676268/151‑1 (MQ=255)
                   tgctTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTCCTTTTGGAGGGGCTGAAAGATGAATGACTGTCCacatg                                                                                                                             >  5:1790981/1‑148 (MQ=255)
                    gctTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCacgac                                                                                                                             <  5:214906/150‑1 (MQ=255)
                        gcgCCAGTGCGCGATTGCTGGCCTAGATGAATCACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCG‑GTTCGGCGGGTTTTTGCTTTTGGAGGGGCACAAAGATGAATTACTGTCCACGACGCta                                                                                                                         <  6:1500591/148‑1 (MQ=21)
                        gcgCCAGTGCGCGATTGCGGGCCTAGATGAACGACTGGCCCCGACAGACCCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAACCCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCATAAAGATGAATGACTGTCCACGACGCtat                                                                                                                        <  4:1167591/149‑1 (MQ=18)
                        gAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGATTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCG‑CTTAGGCGGGTTTTTGCTTTTGGATGGGCAGAAAGATGAATGACTGTCCACGACGCtct                                                                                                                        >  3:1712424/1‑149 (MQ=255)
                         cgcgagtgacgtaTTGCTGGCCTAGATGAATGTCTGTCCCCGACAGAACCCGGCTTATCGTTCTGTTTCACCTTATTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGTTTTTGGTGGGGCTTAAAGATGAATGACTGTCCACGACGCtata                                                                                                                       <  4:818543/139‑1 (MQ=18)
                         aGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGCCAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGCGGGGCAGAAAGATGAATGACTGGCCACGACGCtata                                                                                                                       <  3:509224/151‑1 (MQ=255)
                            cAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTGACCTGATTTACGTAAAAACCCG‑CTTCGGCGGGTTTATGCTTTTGGAGGGGCATATAGAGGAAGCGGTGTCCCCGTCGCtatcgcc                                                                                                                    >  7:485043/1‑147 (MQ=21)
                            cAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGGTTCTCCTGATATAACTAAATACCAC‑CTTCCGCCGGTGTTTTCTTTTGGAGGGGGAGAAAGATGAATCACTGTCCACGACGCTATcccc                                                                                                                    >  7:63842/1‑151 (MQ=21)
                                 aGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGCTGTAGCTAAAAACGCT‑GATCTCCCGTCTGATGGCATTGGAGCGGCAGAAAGATGCTTGGCTGTCAACTACGGTATACCCata                                                                                                                 >  1:1662347/1‑147 (MQ=255)
                                        ggtGGCCTACAGGATTTACGGTCCCCGACAGAAGCGGTCTTTTGGGTCTGTTTCCCGTGATTTACTTAAAACCGCG‑CTTGGGCGGGTTTTTTGTTTGGGCGGGGGATAATGAGGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCg                                                                                                         <  1:493282/148‑1 (MQ=255)
                                         ggggCCTAGATGAATGACTGTCCACGACAGAACCCGGGTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCATAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGc                                                                                                       <  2:1831630/149‑1 (MQ=255)
                                          gggCCTAGATGAATGACTGGCCACGACATAACCCGTCTTATCGGTCAGTTTCACCTGATTTACGTAAAACCCCG‑GTTCGGCGGTTTTTTGCTTTTGGCGGGGCATAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCt                                                                                                      <  8:182160/150‑1 (MQ=17)
                                          gggCCTAGATGAATGACTGGCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACTTAAAAACCCG‑CTTCGGGGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCt                                                                                                      <  1:891743/150‑1 (MQ=255)
                                           ggCCTAGATTAATGACTGGCCACGACAGAACCCGGCTTATGGGTCATTTTCACCTGATTTACGTAAAAACCCG‑CTTCGGCGGTTTTTTGCTTTTGTAGGGTCAGAAACATGATTCACTGTCCACGACGCTATACCCAAAAGAAAGCGGCtt                                                                                                     <  2:450337/151‑1 (MQ=17)
                                           ggCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAACCCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGc                                                                                                       <  1:347881/149‑1 (MQ=255)
                                           ggCCTAGATGAATGACTGTCCACGACACAAACCCCAAAATCACTCAGATTCACCTAATATACGAAAAAACCCA‑AATAAGCAAATTATTACTTTTCCAGAGGCAGAAAGAAGAATAAATAACAACAACACTATACACAAAACAAAGCCGca                                                                                                      >  8:689960/1‑149 (MQ=255)
                                            gCCTAGATTAATGACTGTCCACGACATAACCCGGCTTATCGGTCAGTTTCACCTTATTTACGTAAAAACCCG‑CTTCGGCGGTTTTTTGCTTTTGGAGGGGCATAAAGATTAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTa                                                                                                    <  6:1377854/151‑1 (MQ=17)
                                               tAGATGAATGACTGTCCACGACAGAAAACCCAAAATAAATCATATTAACATCATTTACGTAAAAAACAG‑CTTAAGCAGTTTTTTACTTATCGATGGTCACAAAAATGAATAAATATAAACGACACTATACCCAaaagaaaagagcggaac                                                                                                  >  2:107976/1‑140 (MQ=2)
                                                aGATGAATCACTGGCCCCGACAGAACCCGGCTTATCGGTCATTTTCACCTTATTTACTAAAAACCCCG‑GTGCGGCGGGTTTTTGGTTTTGGCGGGGAATAAATATGATTGACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCg                                                                                                 <  3:851474/150‑1 (MQ=17)
                                                 catgaatcaCTGGCCAGGACAGAACCGGGGTTATCGGGCAGTTTCACCTGATTTACGTAAAACCCGG‑CTTCGGCGGGTTTTTGGTTTTGGTGGCGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGt                                                                                               <  3:731848/150‑1 (MQ=14)
                                                   aacccatctctCCACAACATAACCCGGCTTATCGGTCAGTTTCACTTTTTTTATTTAAAAACCGG‑CTTGGGGGTTTTTTTTTTTTTGTAGGTGCATAAATTTTAATTACTGTCCACGACTCTATACCCAAAAGAAAGCGGCTTATCGGTCa                                                                                             <  6:1728439/141‑1 (MQ=2)
                                                    taatgaCTGGCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCa                                                                                             <  1:1073301/149‑1 (MQ=255)
                                                     aatgaCTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACGTGATTTAGGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGt                                                                                           <  5:992538/151‑1 (MQ=255)
                                                     aatgaCTGGCCACGACAGAACCCGGATTATCGGTCATTTTCACCTAATTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAATATCAATGACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGt                                                                                           <  7:1344265/151‑1 (MQ=17)
                                                        gaCTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTTCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCg                                                                                                         >  4:1242481/1‑134 (MQ=255)
                                                        gaCTGGCCACGACAGAACCGGGCTTATCGGGCAGTTTCACGTGATTTACGTAAAACCCGG‑CTGCGGCGGGTTTTTGTTTTTGTGGGGGCCCAAATATGAATGACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTc                                                                                        <  7:346691/151‑1 (MQ=14)
                                                        gaCTGGCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTc                                                                                        <  1:441243/151‑1 (MQ=255)
                                                           tGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGatgaatg                                                                                                                                         >  5:1890188/1‑99 (MQ=255)
                                                           tGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAACAAGAATGACTGTCCACTACGCTATACCGAATAGAAATAGTCTTATAGTTCATTTTCAcc                                                                                     >  4:1951334/1‑151 (MQ=14)
                                                            ggccACGACATAACCCGGCTTATGGGTCAGTTTCACCTGATTTACTAAAAACCCCG‑CTTCGGCGGGTTTTTGCTTTTGGTGGGGCCGAAACATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCt                                                                                    <  1:226156/149‑1 (MQ=14)
                                                                cccacacAACCCGGCTTAGCGGTCAGTTGCACCTGATTTACGTAAAACCCGG‑CTGCGGGGGGTTTTTGCTTTTGGTGGGGCTTAAATATGAAGGACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGtt                                                                                <  1:1304230/148‑1 (MQ=14)
                                                                accaCAGAACCCGGCTTATCGGTCAGTTTCACGTGATTTACTTAAAACCCCG‑CTTCGGCGGGTTTTTGGTTTTGGTGGGGCAGAACTTTGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGtt                                                                                <  3:1616226/148‑1 (MQ=14)
                                                                 cGACAGAACCCGGATTAGGGGGCAGTTGCCCCTGATTTACGTAAACACCCG‑CTGCGGCGGGTTTTTGCTTTTGGCGGGGCCCAACGATGAAGGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGttt                                                                               <  3:70481/151‑1 (MQ=14)
                                                                 acacacAACCCGGCTGATCGGGAAGTTTCACCTGATTTAGGTAAAACCCGG‑CTTCGGCGGGTTTTTGGTTTTGCCGGGGCCAAAAGTTGAATGCCTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGttt                                                                               <  1:195697/149‑1 (MQ=14)
                                                                  tactgAACCCGGCTTATCGGTCATTTGCTCCTGATTACCTTACAAACGCT‑CTTCTCGGGGTTTTTGCTATTGTTGGCGTCCTAAGTTGAATGACTCTCCTCTTCCCTATACCCAATAGAAAGCGGCTTATCGGTCAGTTTCACCTGGttt                                                                               <  2:346450/146‑1 (MQ=2)
                                                                  caCAGAACCCGGATTAGGGGGAAGTTGCCCCTCA‑TTACCGAAAAACCCGCCTGCGGCGGGTTTTTGGTTTTGCTGGCGCCCAACCATGAATGACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTa                                                                              <  1:570544/150‑1 (MQ=2)
                                                                    cAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTGCGAAAAAACCCC‑CTGCTGGGCGGTTTTGCATAAGGACCGGCACTAAGATGAATGACTGTGCTCGAAAATAAACCCAAAAGAAAGCGGCGTAGCGGGCCGGTTCACGTGGTTTGCg                                                                            >  1:1746684/1‑151 (MQ=2)
                                                                     aGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCG‑CTTCGGCGGGTCTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATAccc                                                                                                                    >  1:114330/1‑110 (MQ=255)
                                                                      caaCCCGGCGTATGGGTCTTTTTCTCCTGATTT‑CGTTAAACCCGGTCTGCTGCGGGTTTTGGGTTTTGGTGGGGGGTAATTTTGATTGACTTTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTa                                                                          <  4:1098177/150‑1 (MQ=9)
                                                                        aCCCGGCTTATGGGTCAGTTGCACCTGATTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGTAGGGGCATAAAGATGAATGCCTCTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTaaa                                                                        <  5:1687177/151‑1 (MQ=14)
                                                                        aCCCGGCTTATCGGGCATTTTCCCGTGATTTAGGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGCAGGGGCATAATCATGAATCACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTaaa                                                                        <  5:1184956/151‑1 (MQ=14)
                                                                                 aTCGGTCAGTTTCACCTGATTTACGTAAAAACCCG‑ATACGACATCTTTTTACATTTGTAGGGGCATAAAGATGAATGACTTTCCAAGAAACTATACCCAAAAGAAAGATTATTATAAGTCAGTTTTACCTGATTTACGTAATAAACCggtg                                                               >  8:793853/1‑148 (MQ=255)
                                                                                 aTCGGTCAGTTTCACCTGATTTACGTAAAAACCCG‑ATAACGCAAGATATTCCTTTTGGAAAAGCAGAAAAATAAATGACTATAAAACACTCTATACCCAAAAAAAAAAAAATTATAGGTCAGTTTCAGCTGGTTTACGTAAAAACCCGcgg                                                               >  2:1528761/1‑149 (MQ=255)
                                                                                 aTCGGTAATTTTCTCCTGATTTTGTTAAAAACGTT‑CTTCTGCGGGTTTTTGTTTTTGTTGGTGCTTAATTTTGATTGACTTTCCAAGACTCTATACACAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCtt                                                               <  8:1292437/151‑1 (MQ=2)
                                                                                   ggggaattttCACCTGATTTACTTAACCACCGT‑CTTCGGCGGGTTTTTGGTTTTGTTGGCGCTCAACTTTGATTGCCTTTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTc                                                              <  8:1516949/143‑1 (MQ=2)
                                                                                   cGGTAAGTTTCCCCTGATTTACGTAAAACCCCG‑CTTCGGCGGGTTTTTGCTTTTGGCGGGGCATAAAGATGAATGCCTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTc                                                              <  5:41920/150‑1 (MQ=11)
                                                                                    ggTCAGTTTCACCTGATTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGGTGTACGTAATAACCCGGTGcgg                                                            >  1:1859217/1‑151 (MQ=255)
                                                                                     gtaattttCACGTTATTTAGTTAAAAACCGT‑CTTCGGCGGTTTTTTGTTTTTGTAGGTGTTTAATTATGATTGACTTTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTcggc                                                           <  4:642165/146‑1 (MQ=11)
                                                                                        aGTTTCACCTGATTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTGATCGGTCAGTTTCACGTGTTGTACGTAATAAACCGCTTCGGCggg                                                        >  5:270936/1‑151 (MQ=11)
                                                                                        aGTTTCACCTGATTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGCCAGAAAGATTAATGAATGTCAACGACGCTATAACCAAAAGAAATCGGCTTATCGGTCAGATTAACCTGATTTACGTAAAAAACCGATACGGCggg                                                        >  6:684040/1‑151 (MQ=11)
                                                                                          tttCACCTGATTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTCGGTTTGGAGGGGCAGAAAGATCAATGACTGGCCACAAAGATATACCCAAAAGAAAGCGGCTTATCGCAAAGTTTCCCCTGGTTTACGTAATAACCCGCGTCgggggggt                                                      >  3:908321/1‑149 (MQ=11)
                                                                                           tgcccttGATTTA‑GTTAAAACCCGTCTGCGGCGGGTTTTTGTTTTTGTTGGTGTTTAATTATGATTGACTTTCCACGACTCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGtt                                                      <  2:1431754/144‑1 (MQ=2)
                                                                                           gtcccatcaTTTACTTAAACCCGCG‑CTTCGGGGGGTTTTTGCTTTTGCCGGGGCACAACCATGATTGACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGttt                                                     <  7:186541/143‑1 (MQ=11)
                                                                                              gcttcaTTTACGTAAAACCGGT‑CTTCGGCGGGTTTTTGTTTTTGTTGGGGGTGAATTTTGAATGACTTTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTg                                                  <  6:166438/146‑1 (MQ=11)
                                                                                               ccTGATTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGGTTTACGTAATAACCCGGTCCGGCGGGtttg                                                    >  3:1478440/1‑147 (MQ=255)
                                                                                               ccTCATTTACGTAACCCCCCC‑CTGCGGCGGGTTTTTGCTTTTGCCGGGGCCCAACGATGAAGGCCTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGc                                                 <  5:1005184/151‑1 (MQ=11)
                                                                                                cTGATTTACGTAAAAACCCG‑CTTCGGCGGGTATATCCTAATAGAGTCGAAGAAAGATAAATTAATTTCCAAAACGCTAAACACAAAAGAAAGCGTATAATATATCAAATTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGttggtgcg                                                >  4:393540/1‑145 (MQ=2)
                                                                                                 tGATTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGGTGTACGTAAAAACCCGCTCCGGCGGGTTTCTGCtt                                               >  5:541862/1‑151 (MQ=255)
                                                                                                  gATTTACTTAAAAACGCG‑GTGCGGCGGGTTTTGGTTTTTGTTGGGGCTCAATGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCtt                                               <  6:374191/150‑1 (MQ=11)
                                                                                                   attaagttAAACACCGG‑CTGCGGCGGTTTTTTGGTTTTGTTGGGGCAGAATGATGAATGACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCttt                                              <  8:637402/143‑1 (MQ=11)
                                                                                                    tttcggTATAAACGGG‑CTGCGGCGGGTTTTTGCTTTTGCTGGTGCTTTCTTTTGATTGACTGTCGACTTCGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCtttt                                             <  2:1772656/145‑1 (MQ=2)
                                                                                                    tttACGTAAAAACCCG‑CTTCGGCGGGTTATTGCTTTTAGAGGGGCAGAAAGATAAATGACTCTCCACGACGCTATACAAAAAAGAAAGCGGCTTATCGCTCAGTTTAAACTGATTTACGTAAAAAAACGATAcggcggctataacattttt                                            >  6:814277/1‑138 (MQ=11)
                                                                                                     ttACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGGATCGGCgggggtgtggtgtggc                                           >  1:1577634/1‑138 (MQ=11)
                                                                                                       ccGTAACACCCGG‑CTGCGGCGGGTTTTTGCTTTTGCCGGGGCCCAAAGATGAATGACTCGCCACGACGCTATACCCAAAAGAAACCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGa                                          <  3:1240169/149‑1 (MQ=11)
                                                                                                       aCGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAGGATGAAGGACTGTCCCCGCGGCTATACCCAAAAGAAAGCGGCTGATCGGTAAGTATCACCTGGTTTACGTAAGAACCCGCTGCGGCGGGgtttgggggtggg                                           >  7:1273043/1‑140 (MQ=11)
                                                                                                       aCGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGAGTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGGTTTACGTAAAAACCCGGTCGGGCGGGTTTGTGCTTGTgg                                           >  7:1447156/1‑149 (MQ=11)
                                                                                                       aCGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGGTTTACGTAAAAACCCGCTGCGGCGGGTTTTTGCttgttgtg                                         >  7:723264/1‑145 (MQ=255)
                                                                                                       aCGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTGTTGGAGGGGCAGAAAGCTGAATGACTGTCCCCGCCGCTATACGCAAAAGATAGCGGCGTATCGGTCGGTTTCACCTGGTTTACGTAAAAACCCGCGTCGGCGGGGTGTTGCtggggggg                                         >  3:1292206/1‑144 (MQ=11)
                                                                                                        gttaataaCCGG‑CTTCTGCGTGTTTTTGTTTTTGGTGGGGCTTATTGTTGAATGACTGTCGAAGACGCTATACACAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAgg                                        <  8:1938586/149‑1 (MQ=11)
                                                                                                          taaaccccgg‑CTGCGGCGGGTTTTTGTTTTTGCTGGGGCCTAACTTTGATTGACTGGCCAAGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAggg                                       <  4:501407/141‑1 (MQ=11)
                                                                                                          tAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCGGAAAGATGAATGACTGTCCACGACTCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGGTGTACGTAATAACCCGGTCCGGCGGGTTTCTGCTTTTTGAgtgg                                      >  3:1197637/1‑151 (MQ=11)
                                                                                                            aatatcgttcTTAGGCGGGTTTTTGTTTTTGTTGGTGTTTTATTATGATTGACTTTAGTCTACGCTTTACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGc                                     <  6:1944860/142‑1 (MQ=2)
                                                                                                            aaacCCCG‑CTTCGGCGGGTTTTTGGTTTTGGGGGGGCCCAAACATGAATGACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCa                                    <  7:1692543/151‑1 (MQ=11)
                                                                                                                cccG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGGTTTACGTAATAACCCGGTACGGCGGGTTTCAGCTTGTTGAGTGGCAGat                                 >  7:913548/1‑149 (MQ=11)
                                                                                                                  cG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGGTTTACGTAAAAAACCGATGCGGCGGGTTTGAGCTTGTGGAGTGGGAgttatt                              >  7:1034778/1‑146 (MQ=11)
                                                                                                                       gagccggGTTTTTGCTTTTGGCGCCGCACAACCATGAATGCCTCGCCACCACGCTATACCCAACAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGatg                            <  5:1093821/145‑1 (MQ=11)
                                                                                                                        atgcggGTTTTTGTTTTTGTAGGCGCTTAACATTGATTGCCTCGCGACGACTCTATACACTACAGAAAGCGGCTTATCGGTCAGTTTCACCTGCTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGatgaat                         <  6:1294509/149‑1 (MQ=11)
                                                                                                                        aggcggGTTTTTGTTTTTGTTGGTGCACAATTTTGATTGACTTGATAAGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGatgaat                         <  2:944440/150‑1 (MQ=11)
                                                                                                                         ggcggGTTTTTGTTTTTGTCGGGGCTCTACTATGCAGGACTTTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGatgaatg                        <  8:860402/151‑1 (MQ=11)
                                                                                                                                                gCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTAGGAGGGTAAGAAAGACGAATGACGGTcccccacccgagagcca  >  3:221376/1‑135 (MQ=11)
                                                                                                                                                       |                                                                                                                                              
GTACTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCA  >  DH1/613451‑613743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑