Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1613,6130TG27.5% 245.8 / 46.8 246intergenic (+474/+549)garK/yhaCglycerate 2‑kinase/pentapeptide repeats‑related protein
Reads supporting (aligned to +/- strand):  ref base T (62/109);  new base G (1/64);  total (67/179)
Fisher's exact test for biased strand distribution p-value = 1.56e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 250 total aligned reads displayed.

GGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTAT  >  DH1/613463‑613759
                                                                                                                                                         |                                                                                                                                                  
ggtttggttgttGAGGCAGTGAGCTATTGATGGACTAGATCACACACTCTACACCAAAGAAACCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTTCTTTTGGAGGGGCAGAAAGATGAATGACTGt                                                                                                                                                    <  3:1644425/141‑1 (MQ=11)
 gTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTc                                                                                                                                                   <  7:764238/151‑1 (MQ=255)
  tAGTCTGCTTGAGCCCGCGAAATATTGATGGCCTAGAGCACACACTCTACACTAAAGAACGCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTc                                                                                                                                                   <  3:1829838/150‑1 (MQ=11)
   aGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTcc                                                                                                                                                  <  6:1090500/150‑1 (MQ=255)
   aGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCa                                                                                                                                                 <  3:900153/151‑1 (MQ=255)
   aGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCa                                                                                                                                                 <  1:1793311/151‑1 (MQ=255)
   aGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCa                                                                                                                                                 <  2:171085/151‑1 (MQ=255)
   aGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCa                                                                                                                                                 <  3:1110239/151‑1 (MQ=255)
    ggcggcTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCCCGACAGAAACCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAACCG‑C‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCac                                                                                                                                                <  8:1721573/151‑1 (MQ=255)
    ggCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCac                                                                                                                                                <  1:676268/151‑1 (MQ=255)
    ggCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCac                                                                                                                                                <  4:477363/151‑1 (MQ=255)
    ggCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCac                                                                                                                                                <  4:1563172/151‑1 (MQ=255)
    ggCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGCCAGAAAGATGAATGACTGTCCac                                                                                                                                                <  5:725345/151‑1 (MQ=255)
     gctgctTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCacg                                                                                                                                               <  7:1299097/151‑1 (MQ=255)
     gcgtctttcgcaaGTGAGCGATTGCTGGCCTAGATTAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCacg                                                                                                                                               <  6:667023/139‑1 (MQ=255)
       tgctTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑A‑G‑CTTCGAAGAGATTATGCTATTGAAGGCGCATAAAGATGAATGACTATCAacgac                                                                                                                                             >  2:226052/1‑151 (MQ=25)
            gAGCCTGTGAGCGATTGCTGGCCTAGATGAATCACTGTCCACGACATAACCCGGCTTATCGGGCAGTTTCACCTGATTTACTTAAAAACC‑C‑G‑CTTGGGGGGGTTTTTGCTTTTGGAGGGGCACAAAGATCAATGACTGTCCACGACGCtat                                                                                                                                        <  8:466293/151‑1 (MQ=21)
             aGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑A‑G‑CTACGAAGAGATTATTGTTTTGCAAGCGAATAAAGATTAATGAATATCAACGAGGatat                                                                                                                                        >  8:1321155/1‑150 (MQ=19)
              gCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTAAGAAAAAGAC‑A‑G‑ATCACGCGTGTGGTGTCTTTTGCACGGGCAGAAACATGAATGACTGAAAAAGAAGCTATAc                                                                                                                                      >  3:332766/1‑151 (MQ=255)
               ccAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑A‑G‑CTTCGGCGGGATATTGCTATAGCATCGGCAAAAAGATGAATGAATGTAAAAGACGAtataac                                                                                                                                     >  4:1481234/1‑149 (MQ=21)
                cAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTGACCTGATTTACGTAAAAACC‑C‑G‑CTTCGGCGGGTTTATGCTTTTGGAGGGGCATATAGAGGAAGCGGTGTCCCCGTCGCtatcgcc                                                                                                                                    >  7:485043/1‑147 (MQ=21)
                cAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGGTTCTCCTGATATAACTAAATACC‑A‑C‑CTTCCGCCGGTGTTTTCTTTTGGAGGGGGAGAAAGATGAATCACTGTCCACGACGCTATcccc                                                                                                                                    >  7:63842/1‑151 (MQ=21)
                 aGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACACAAAACATCTTATCAGACACTTTAACCTAAATTAAGTAAAAAAA‑A‑G‑ATTAGAACTCTTTTTTCTATTGAAGATTAAAAAAGATGAATGACTGTCGACGACACtatagaa                                                                                                                                    >  4:1210442/1‑147 (MQ=255)
                   tGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAAAC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGTATGACTTTCGACGACCCttgcggcta                                                                                                                                  >  7:904506/1‑142 (MQ=21)
                     aGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGCTGTAGCTAAAAACG‑C‑T‑GATCTCCCGTCTGATGGCATTGGAGCGGCAGAAAGATGCTTGGCTGTCAACTACGGTATACCCata                                                                                                                                 >  1:1662347/1‑147 (MQ=255)
                      gCGATTGCGGGCCTAGATGAATGCCTGTCCACGACAGAACCCGGCTTATCGGGCAGTTTCACCTGATTTACTTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAaaaga                                                                                                                              <  4:1107258/151‑1 (MQ=255)
                        gATTGCTGGGATAGATGAATGTCTGTCCAGGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATTAATGACTGTCCACGACGCTATACCCAaaagaaa                                                                                                                            <  2:872842/151‑1 (MQ=255)
                        gATTGCTGGCCTAGATGAATGACTGCCCACGACATAACCCGGCTTTTCGGTCATTTTCACGTGATTTACTTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGGCCACGACGCTATACCCAaaagaaa                                                                                                                            <  5:1142603/151‑1 (MQ=18)
                         aTTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGCCTTATAGGTCAGATTCACCTGAATTAAGTAAAAACC‑A‑G‑CTTCGGCTACTATTTGCTTTTGGATGGGCAGAAACATGAATTAATATCAACGACACTATACCCAaaagaa                                                                                                                             >  8:823033/1‑149 (MQ=18)
                           tGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCATTTTCACCTGATTTACGTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGTTTTTGGAGGGGCAGAAAGATTAATGACTGTCCACGACGCTATACCCAAAAGAAAGc                                                                                                                          <  2:546502/150‑1 (MQ=255)
                            ggtGGCCTACAGGATTTACGGTCCCCGACAGAAGCGGTCTTTTGGGTCTGTTTCCCGTGATTTACTTAAAACCG‑C‑G‑CTTGGGCGGGTTTTTTGTTTGGGCGGGGGATAATGAGGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCg                                                                                                                         <  1:493282/148‑1 (MQ=255)
                             gtGGCCTAGATGAATGACTGCCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTGTAGGGGCAGAAAGATTAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGc                                                                                                                       <  6:1648960/150‑1 (MQ=255)
                             ggggCCTAGATGAATGACTGTCCACGACAGAACCCGGGTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCATAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGc                                                                                                                       <  2:1831630/149‑1 (MQ=255)
                              gggCCTAGATGAATGACTGGCCACGACATAACCCGTCTTATCGGTCAGTTTCACCTGATTTACGTAAAACCC‑C‑G‑GTTCGGCGGTTTTTTGCTTTTGGCGGGGCATAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCt                                                                                                                      <  8:182160/150‑1 (MQ=17)
                              gggCCTAGATGAATGACTGGCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACTTAAAAACC‑C‑G‑CTTCGGGGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCt                                                                                                                      <  1:891743/150‑1 (MQ=255)
                               ggCCTAGATGAAGCACTGGCCACGACAGAACCCGGCTTAGCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑G‑CTTCGGGGGGTTTTTGCTTTTGGAGGGCCAGAAAGATGAATTACTGTCCACGACGCTATACCCAAAAGAAAGCGGCtt                                                                                                                     <  2:1073327/151‑1 (MQ=17)
                                 ccTAGATGAATGACTGGCCACGACAGAACCCGGCTTATCGTGCAGTTTCACCTTATTTACGTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTAt                                                                                                                   <  3:1838387/151‑1 (MQ=255)
                                    aGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATc                                                                                                                  <  3:1001471/149‑1 (MQ=255)
                                    aGATGAATCACTGGCCCCGACAGAACCCGGCTTATCGGTCATTTTCACCTTATTTACTAAAAACCC‑C‑G‑GTGCGGCGGGTTTTTGGTTTTGGCGGGGAATAAATATGATTGACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCg                                                                                                                 <  3:851474/150‑1 (MQ=17)
                                     catgaatcaCTGGCCAGGACAGAACCGGGGTTATCGGGCAGTTTCACCTGATTTACGTAAAACCC‑G‑G‑CTTCGGCGGGTTTTTGGTTTTGGTGGCGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGt                                                                                                               <  3:731848/150‑1 (MQ=14)
                                      attaattaCTGGCCACTAAATAACCCGTGTTATGGGTTATTTTCACGTGATTTCCTTAAAACGC‑G‑T‑CTGCGGGGGGTTTTTGCTTTTGGTTGGGCCCAAAGATTAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCTGt                                                                                                               <  5:1029693/147‑1 (MQ=2)
                                      atgaatgaCTGTCCACGACATAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGt                                                                                                               <  1:1880149/150‑1 (MQ=255)
                                        taatgaCTGGCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCa                                                                                                             <  1:1073301/149‑1 (MQ=255)
                                            gaCTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTTCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCg                                                                                                                         >  4:1242481/1‑134 (MQ=255)
                                                gTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACACAAAAGAAAGAGGCTTATCAGACAGTATGAccg                                                                                                    >  2:446826/1‑150 (MQ=14)
                                                 gccaccaCAGAACCCGGCTTATCGGTCAGTTGCACGTGATTTCCTTAAAACCC‑G‑G‑CTGCGGCGGGTTTTTGTTTTTGGCGGGGAATAATCATGAATGACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTg                                                                                                   <  6:873136/150‑1 (MQ=14)
                                                  ccaccaCAGAACCCGGCTTATGGGGCATTTTCACGTGATTTACTTAAAACCC‑G‑G‑CTGCGGCGGGTTTTTGTTTTTGGTGGGGCCTAAACTTGATTGCCTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTgg                                                                                                  <  6:365006/151‑1 (MQ=14)
                                                    cccacacAACCCGGCTTAGCGGTCAGTTGCACCTGATTTACGTAAAACCC‑G‑G‑CTGCGGGGGGTTTTTGCTTTTGGTGGGGCTTAAATATGAAGGACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGtt                                                                                                <  1:1304230/148‑1 (MQ=14)
                                                     ccacacAACCCGGATTATGGGCCAGTTGCCGCTGATATCCTTAAACCCG‑C‑G‑CTGCCGCGGGTTTTTGCTTTTGCCGGCGCCCAACCATGAATGCCTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGttt                                                                                               <  3:1722510/149‑1 (MQ=9)
                                                     cGACAGAACCCGGATTAGGGGGCAGTTGCCCCTGATTTACGTAAACACC‑C‑G‑CTGCGGCGGGTTTTTGCTTTTGGCGGGGCCCAACGATGAAGGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGttt                                                                                               <  3:70481/151‑1 (MQ=14)
                                                      gACAGAACCCGGCTTATCGGTCAGTTTCACCTGAATAACGTAAAAACC‑A‑C‑CTTATGCGGAATTTTGGTTATATATGGGGAGAAATATGAATGACTCTCAACAAAAAAAAAAAAAAAAGAAAGCGGCTTATCGGTCAGTTTGCCCTggagtt                                                                                              >  6:1381840/1‑147 (MQ=2)
                                                      caCAGAACCCGGATTAGGGGGAAGTTGCCCCTCA‑TTACCGAAAAACC‑C‑GCCTGCGGCGGGTTTTTGGTTTTGCTGGCGCCCAACCATGAATGACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTa                                                                                              <  1:570544/150‑1 (MQ=2)
                                                       aCAGAACCCGGTTTAGCGGTAAGTTTCACCTGATTTACTTAAAACCG‑G‑T‑CTGCGGCGGGTTTTTGCTTTTGTTGGGGCCCAAACATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTAc                                                                                             <  5:975432/151‑1 (MQ=14)
                                                        cAGAACCCGGCTTATCGGGCATTTGCCCGTGATTTACTTAAAACCC‑G‑G‑CTGCGGCGGGTTTTTGGTTTTGGGGGGGCATAACCATGAATGACTTTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACg                                                                                            <  3:1208766/151‑1 (MQ=14)
                                                        aaGAACCCGGCTTATCGGTCATTTTCACCTGATTTACGTAAAACCC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCACAAAGATGAATGACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACg                                                                                            <  7:465719/150‑1 (MQ=255)
                                                         aGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑G‑CTTCGGCGGGTCTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATAccc                                                                                                                                    >  1:114330/1‑110 (MQ=255)
                                                          gaccgggggttAGCGGTCTGTTGCACCTGA‑TTACGTAAACACCGT‑C‑CTGCGCCGGGTTTTTGTTTTTGCCGGCGGCTCAGCATGATGGCCTCGACACGACGCTCTACCCAAAAGAAACCGGCTTATCGGTCAGTTTCACCTGGTTTACg                                                                                            <  3:1138658/140‑1 (MQ=2)
                                                            aCCCGGCTTATCGGGCATTTTCCCGTGATTTAGGTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTGCAGGGGCATAATCATGAATCACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTaaa                                                                                        <  5:1184956/151‑1 (MQ=14)
                                                            aCCCGGATTATGGGGAATTTTCCCGTGATTTCCTTCACACCG‑C‑T‑CTTCGGCGGGTTTTTGGTTTTGACGCCGCCCAAGTTTGATTGACTTGCCAAGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTaaa                                                                                        <  1:1366433/151‑1 (MQ=2)
                                                             ccgggattATCGGAAATTTTCCCGTGATTTAGTTAAAACCG‑C‑T‑CTTCGGGGGGTTTTTGTTTTTGTAGGGGCATAAATTTGATTGACTGTACAAGACTCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTaaaa                                                                                       <  4:1015969/145‑1 (MQ=2)
                                                             cccGGCTTATGGGTAAGTTTCAGGTGATTTTCTTAATAACG‑G‑G‑CTTGGGCGGTTTTTTGTTTTTGTTGGGGCATAATTATGATTGACTCGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTaaaa                                                                                       <  5:1692264/151‑1 (MQ=2)
                                                               cGGATTATGGGGCAGTTTCCCGTGATTTACGTAAAACCC‑G‑T‑CTTCGGCGGGTTTTTGCTTTTGCTGGCGCAGAAACATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAAc                                                                                     <  5:242789/151‑1 (MQ=11)
                                                                gggttATCGGTCATTTTCAGCTGTTTTTGGTCAACACC‑C‑G‑CTTGGGCGTGTTTTTGGTTTTGTGGGTGCCCAACCTTGATTGCCTCGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAAcc                                                                                    <  1:223981/148‑1 (MQ=2)
                                                                ggCTTATGGGTAAGTTGCACTTTATTTACTTAAAAACG‑T‑T‑CTTCTGCGGGTTTTTGGTTTTGATGGTGGTTAAATATGAATGACTTGCCACGTCTCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAAcc                                                                                    <  4:848670/151‑1 (MQ=2)
                                                                  attaggggTAAGTTGCCCGTGATTTAGTTAAACCCG‑G‑G‑CTGCGGCGGTTTTTTGGTTTTGGGGGGGCAAAACATTGATTGACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCg                                                                                  <  7:705726/145‑1 (MQ=2)
                                                                   ttATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATCAATGACTTTACAAGACGATATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCAGCTGATTTACGTAAAAACCCGc                                                                                 >  7:1402318/1‑151 (MQ=11)
                                                                    tATGGGTAATTTTCCCTTGATTTACTTAAAAACC‑T‑T‑CTTCGGCGGGTTTTTGTTTTTGTTGGTGTTTAAATTTGATTGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGc                                                                                 <  6:1365415/150‑1 (MQ=11)
                                                                    tATGGGTAATTTTCCCCTGATTT‑CGTTAACCCC‑GGG‑CTTCTGCGGGTTTTTGCTTTTGTTGGGGCCCAAATATGATTGACTTTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGc                                                                                 <  6:1665039/150‑1 (MQ=2)
                                                                    tATCTGTAATTTTCCCCTTATTTACGTAAAACCC‑C‑C‑CTTCGGCGGTTTTTTGTTTTTGGCGGCGAATAATTTTTATTGACTTTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGc                                                                                 <  7:285973/150‑1 (MQ=11)
                                                                     aTCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑G‑ATAACGCAAGATATTCCTTTTGGAAAAGCAGAAAAATAAATGACTATAAAACACTCTATACCCAAAAAAAAAAAAATTATAGGTCAGTTTCAGCTGGTTTACGTAAAAACCCGcgg                                                                               >  2:1528761/1‑149 (MQ=255)
                                                                       gggaaatttgccccTCATTTAC‑TAAAAACC‑CGG‑CTTCGGCGGGTTTTTGTTTTTGCTGGGCCCAAAAGATCAATCCCTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTcg                                                                             <  5:442149/139‑1 (MQ=11)
                                                                       gggTAAGTTTCTCGTGATTTACTTAAAACCC‑C‑T‑CTGCGGCGGGTTTTTGCTTTTGTTGGGGCCTAACGATGATTGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTcg                                                                             <  5:274117/150‑1 (MQ=11)
                                                                        gttCAGTTTCACCTGATTTACGTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGtt                                                                                                          >  5:1568551/3‑121 (MQ=21)
                                                                        gtcgatttcgagcTGATTAACTAAAACACC‑G‑T‑CTTCGGCGGGTTTTTGGTTTTGCCGCCGCTCAACCTTGAATGCCTTGACAACACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTcgg                                                                            <  1:1516477/139‑1 (MQ=2)
                                                                               tgcccttGATTTA‑GTTAAAACC‑C‑GTCTGCGGCGGGTTTTTGTTTTTGTTGGTGTTTAATTATGATTGACTTTCCACGACTCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGtt                                                                      <  2:1431754/144‑1 (MQ=2)
                                                                               gtcccatcaTTTACTTAAACCCG‑C‑G‑CTTCGGGGGGTTTTTGCTTTTGCCGGGGCACAACCATGATTGACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGttt                                                                     <  7:186541/143‑1 (MQ=11)
                                                                                 caccgcaTTTACGTAACACCC‑C‑G‑CTGCGGCGGGTTTTTGGTTTTGCCGGGGCCGAAAGATGAATGACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGttttt                                                                   <  1:431513/145‑1 (MQ=11)
                                                                                 cACCTGATTTACGAAAACCCC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCACAAAGATGAAGGACTCGCCACGACGCTATCCCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGttttt                                                                   <  1:17231/151‑1 (MQ=11)
                                                                                  cccTCATTTCCGTAAACCCC‑C‑C‑CTGCGGCGGGTTTTTGCTTTTGCAGGGGCCCACACATGAACGACGCCCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTg                                                                  <  5:1830398/150‑1 (MQ=11)
                                                                                   ccTGATTTACGTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGGTTTACGTAATAACCCGGTCCGGCGGGtttg                                                                    >  3:1478440/1‑147 (MQ=255)
                                                                                   ccTGATTTACGTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAAATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGAGGCTTATAGTTCAGTTTCACCTGGTTTAAGTAAAAACCCGCTTCGGCGGGTTTTTGc                                                                 >  8:703760/1‑151 (MQ=255)
                                                                                      gATATACGTAAAAACC‑C‑G‑CTTCGGGGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAAGCCCTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCt                                                                <  1:238066/149‑1 (MQ=11)
                                                                                       aTTTCCGAAACAACC‑G‑G‑CTTCGGCGGGTTTTTGGTTTTGGCGGGGCCCAAACATGAATCACTCTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCtttt                                                             <  7:161249/151‑1 (MQ=11)
                                                                                        tttcggTATAAACG‑G‑G‑CTGCGGCGGGTTTTTGCTTTTGCTGGTGCTTTCTTTTGATTGACTGTCGACTTCGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCtttt                                                             <  2:1772656/145‑1 (MQ=2)
                                                                                         ttACGTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGGATCGGCgggggtgtggtgtggc                                                           >  1:1577634/1‑138 (MQ=11)
                                                                                           tccgtaaccccgc‑G‑CTGCGGCGGGTTTTTGGTTTTGCCGGCGCCCACCCATGATGGCCTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTgg                                                           <  3:1569448/138‑1 (MQ=11)
                                                                                           aCGTAAAACCC‑C‑T‑CTTCTGCGGGTTTTTGCTTTTGGTGGTGTATAAATATGATTGACTGTCCACGACGCTATACACAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGa                                                          <  8:683998/150‑1 (MQ=11)
                                                                                           aCGTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAGGATGAAGGACTGTCCCCGCGGCTATACCCAAAAGAAAGCGGCTGATCGGTAAGTATCACCTGGTTTACGTAAGAACCCGCTGCGGCGGGgtttgggggtggg                                                           >  7:1273043/1‑140 (MQ=11)
                                                                                           aCGTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTGTTGGAGGGGCAGAAAGCTGAATGACTGTCCCCGCCGCTATACGCAAAAGATAGCGGCGTATCGGTCGGTTTCACCTGGTTTACGTAAAAACCCGCGTCGGCGGGGTGTTGCtggggggg                                                         >  3:1292206/1‑144 (MQ=11)
                                                                                            gttaataaCC‑G‑G‑CTTCTGCGTGTTTTTGTTTTTGGTGGGGCTTATTGTTGAATGACTGTCGAAGACGCTATACACAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAgg                                                        <  8:1938586/149‑1 (MQ=11)
                                                                                              aaaaacCC‑C‑G‑CTGCGGCGGGTTTTTGCTTTTGGAGGGGCCCAACGATGAATGACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAgggg                                                      <  5:1978516/150‑1 (MQ=11)
                                                                                                  aatatcgttcTTAGGCGGGTTTTTGTTTTTGTTGGTGTTTTATTATGATTGACTTTAGTCTACGCTTTACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGc                                                     <  6:1944860/142‑1 (MQ=2)
                                                                                                  aaatacggtcTTCGGCGGGTTTTTGTTTTTGTTGGTGTATAAATTTGATTGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAgggg                                                      <  4:652468/141‑1 (MQ=11)
                                                                                                       c‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGGTTTACGTAAAAAACCGATGCGGCGGGTTTGAGCTTGTGGAGTGGGAgttatt                                              >  7:1034778/1‑146 (MQ=11)
                                                                                                       c‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGATGTACGTAAAAACCCGATACGGCGGGTTTCTGCTTGTTGAg                                                         >  3:49189/1‑140 (MQ=11)
                                                                                                              cggcggGTTTTTGTTTTTGTCGGTGCATAATTTTGATGGACTCTACACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGatgaat                                         <  8:1209308/151‑1 (MQ=11)
                                                                                                               ggcggGTTTTTGTTTTTGTTGGTGCTTTATAATGAATGACTTGCCTCTACTCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGatgaatg                                        <  4:613896/151‑1 (MQ=11)
                                                                                                               ggcggGTTTTTGTTTTTGTCGGGGCTCTACTATGCAGGACTTTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGatgaatg                                        <  8:860402/151‑1 (MQ=11)
                                                                                                                     gttttggttttggTGGGGCAGTATGTTGAGTGACTGGCGTCGTCGCTATACCCCACAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTc                                  <  6:693678/150‑1 (MQ=11)
                                                                                                                       tttGGTTTTTGAGGGTGTGAAAGATTCATGCCTTTGCTCCACGCTTTACCCATAATTAACCGGGTTATGGGTCACTTTGACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTcc                                 <  4:199590/150‑1 (MQ=2)
                                                                                                                                                         |                                                                                                                                                  
GGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTAT  >  DH1/613463‑613759

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑