BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	DH1	936,169	0	T	.	48.9%	171.2 / 179.1	93	intergenic (‑13/+21)	ECDH1_RS04615/ECDH1_RS04620	tRNA‑Met/tRNA‑Met
Reads supporting (aligned to +/- strand): ref base T (3/44); new base . (4/41); total (7/86)
Fisher's exact test for biased strand distribution p-value = 7.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01

Only 100 of 123 total aligned reads displayed.

GAATGGCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGG  >  DH1/936019‑936308

gacaggCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTATGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCt                                                                                                                                              <  2:1130293/147‑1 (MQ=255)

gAATGGCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCt                                                                                                                                              <  1:1378545/151‑1 (MQ=255)

caaTGGCGCTTTTGTATGGCAGACCACAAACACAAAAACACGCGATAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCt                                                                                                                                              <  5:1531120/150‑1 (MQ=18)

 attggttcTTTTTTTTGGGAGACCAGTAACATATAAAGATGTTTTTGGTTGTTCATTCATTGGTTGCGGGGGCCGGATTTGAACCGACGAGCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCtt                                                                                                                                             <  2:381541/144‑1 (MQ=14)

 attGGCGCTTTTTTATGTGAGACCAGAAACAAAAAATCACACTTTTGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCt                                                                                                                                              <  2:237879/148‑1 (MQ=18)

  catccgagcttttATGGGAGACCAGAAACAAAAAAACACCCTTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCttt                                                                                                                                            <  1:93048/142‑1 (MQ=255)

  aaggCGCTTTTTTATGGGAGACCAGAAACAAAAAAACCCCCTTTAGGGTGTTCAATCATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCttt                                                                                                                                            <  1:1891877/149‑1 (MQ=255)

  aTGGCTCGTTTTTATGGGAGACCAGAAACAAAAAATCCCGTTTTATGGTGATCATTCATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGACACCGTACTGCttt                                                                                                                                            <  6:506808/151‑1 (MQ=17)

  aTGGCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCttt                                                                                                                                            <  5:1076567/151‑1 (MQ=255)

  aTGGCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCttt                                                                                                                                            <  5:320226/151‑1 (MQ=255)

   aggttcTTTTTTTTGGGAGCCCAGAAAAAAATAAACACTCTTTTTGTTGTTATATTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCttt                                                                                                                                            <  1:1261976/145‑1 (MQ=14)

    ggCGCTTTTTTATGGGAGACCAGAAACAAAAAACCACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTAGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTa                                                                                                                                          <  5:1567129/151‑1 (MQ=255)

    ggCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCtttt                                                                                                                                           <  4:337581/150‑1 (MQ=255)

    ggCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCtttt                                                                                                                                           <  1:1006348/150‑1 (MQ=255)

    ggCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCtttt                                                                                                                                           <  7:244633/150‑1 (MQ=255)

    ggCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCtttt                                                                                                                                           <  5:66728/150‑1 (MQ=255)

    ggCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCtttt                                                                                                                                           <  6:552195/150‑1 (MQ=255)

    ggCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTa                                                                                                                                          <  3:1797704/151‑1 (MQ=255)

    ggCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTa                                                                                                                                          <  6:997042/151‑1 (MQ=255)

     gcgcTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGTTTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTAc                                                                                                                                         <  7:1228296/151‑1 (MQ=255)

      cgcTTTTTTATGGGAGACCAGATACAACTAAACAGCGTTTTGGGTGTTCAATTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCtttactt                                                                                                                                        <  3:199919/151‑5 (MQ=17)

      cgcTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCttttctt                                                                                                                                        >  1:969843/1‑148 (MQ=255)

       gcTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGTGGCAGGATTTGAAACGACGACCTTCGGGTTATGAGACCGACGAGCTACCAGGCTGCTCCACCCCGCGTAACCGTACTGCtttacgtg                                                                                                                                       >  2:1334903/1‑146 (MQ=21)

        cTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTAGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTtc                                                                                                                                      >  6:1455494/1‑150 (MQ=255)

          tttttATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCtttacttaat                                                                                                                                     >  4:1436496/1‑143 (MQ=255)

          tttttATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATGGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTtc                                                                                                                                      <  6:1377545/149‑1 (MQ=255)

          tttttATGGGAGACCAGAAACAAAAAAACACCCGATAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTtca                                                                                                                                     <  7:1891203/150‑1 (MQ=255)

           ttttATGGGAGACCCGAAACAACAAAACCCCCGTGAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTtcatc                                                                                                                                   <  4:1415789/150‑1 (MQ=17)

           ttttATGGGAGACCAGAAACAATAAATCAGCCGTTAGGTTGTTCAATTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTtcatc                                                                                                                                   <  6:764376/150‑1 (MQ=17)

           ttttATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTtcat                                                                                                                                    <  8:277093/150‑1 (MQ=255)

            tttATGGGAGACCCGAAACAAAAAAACCCCCTTTAGGGTGTTCTATTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTtcatca                                                                                                                                  <  6:739802/150‑1 (MQ=17)

            tttATGGGAGACCAGAAACAAAAAAACACCCGTTAGGTTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTtcatca                                                                                                                                  <  3:1428084/150‑1 (MQ=255)

            tttATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCttt                                                                                                                                            >  2:92786/1‑141 (MQ=255)

            tttATGGGAGACCAGAAAAAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTtcatca                                                                                                                                  <  5:728026/150‑1 (MQ=255)

             ttATGGGAGACCAGATACAATAAAAGACCCTTTAGGTTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCaa                                                                                                                                 <  4:275258/150‑1 (MQ=17)

             ttATGGGAGACCAGAACCAAAAACACACCCGTGAGGGTGTTCATTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCaa                                                                                                                                 <  1:1308657/150‑1 (MQ=17)

             ttATGGGAGACCAGAAACAAAAAAACCCCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTtcatca                                                                                                                                  <  5:1903688/150‑1 (MQ=255)

             ttATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTtcatca                                                                                                                                  <  7:1945556/150‑1 (MQ=255)

             ttATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTtcatca                                                                                                                                  <  8:677210/150‑1 (MQ=255)

             ttATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTAATCaa                                                                                                                                 >  4:1364498/1‑150 (MQ=255)

              tATGGGAGACGAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGAGGAGCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCGACCCCGCGTCACCGTACTGCTTTTACTTCATCaa                                                                                                                                 <  6:10999/150‑1 (MQ=255)

              tATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCaa                                                                                                                                 <  4:1079168/150‑1 (MQ=255)

              tATGGGACACCAGAAAAAAAAAAACACCCGAGAGCCTGATAAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCaaa                                                                                                                                <  3:919186/150‑1 (MQ=14)

               attggAGCCCCGGAACCAAAAAACCCGCGTGAGCGTGTTCTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑tt                                                                                                                             <  3:1781291/148‑1 (MQ=11)

               aTGGGAGCCCAGAAAAAAATAAACCCGGGTTAGGTTGTTATATAATTGGTTGCGGCGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑t                                                                                                                              <  3:234791/150‑1 (MQ=14)

               aTGGGAGAGCATAAACAAAAAAACACCCTTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGTATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCaaa                                                                                                                                <  5:1785288/150‑1 (MQ=255)

               aTGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCaaa                                                                                                                                <  1:511194/150‑1 (MQ=255)

               aTGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCaaa                                                                                                                                <  8:701086/150‑1 (MQ=255)

               aTGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑t                                                                                                                              <  5:301709/151‑1 (MQ=255)

                tGGGATACCAGTAACAATTAAAGAGCTTTTAGGTTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑tt                                                                                                                             <  6:1907940/150‑1 (MQ=11)

                tGGGAGACCAGAAAAAACTAAAGAGCGTATATGTTGTTAAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑tt                                                                                                                             <  2:1713290/150‑1 (MQ=11)

                 tgcaaccctcaaaCAACAAATCATCTTTTATGTTTTTAAATAATTGGTTGCTGGGGCCGGAGTTGAACCGACGACCTTCGGGTTATTAGCCCTACGAGCTACAAGGCTTCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑ttt                                                                                                                            <  8:972807/140‑1 (MQ=0)

                 gggAGACCAGAAACAAATACACACCCGTGATGGTGATAAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑ttt                                                                                                                            <  5:102550/150‑1 (MQ=14)

                 gggAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCtttaaataaacaaatttt                                                                                                                             >  6:424172/1‑136 (MQ=255)

                 gggAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑ttt                                                                                                                            <  3:708019/151‑1 (MQ=255)

                 gggAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑ttt                                                                                                                            <  6:358024/151‑1 (MQ=255)

                  tcagaCCTGATACAAAAAAACAGTTTTTTTGGTGTTAATTAATTGGTTGCGGGGGCCGGATTTTAACCGACGACCTTCGGTTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑tttt                                                                                                                           <  6:1746662/148‑2 (MQ=11)

                  ggAGCCCAGCACCAACAAATCACCAGTTAGGGTGTTAAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑tttta                                                                                                                          <  3:1334207/151‑3 (MQ=14)

                  ggAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAC‑TTTg                                                                                                                           <  7:508730/151‑1 (MQ=255)

                  ggAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTg                                                                                                                           <  8:241115/151‑1 (MQ=255)

                  ggAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCGCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTg                                                                                                                           <  6:383415/151‑1 (MQ=255)

                   gagaCCTGATAAAAAAAAACATCTTTTTGGTTGTTAAATTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑ttttaa                                                                                                                         <  2:974692/151‑4 (MQ=11)

                    agcccACAACCAAAAAAACAGCCGTTAGCGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑ttttaat                                                                                                                        <  3:1739539/148‑5 (MQ=14)

                    agaCCAGAAACAAAAAAACACGCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑tttta                                                                                                                          <  5:220212/149‑3 (MQ=255)

                    agaCCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑ttttaat                                                                                                                        >  6:220103/1‑147 (MQ=255)

                    acaCCCGAAACAAAAAAACACCCTTTAGCGTGTTCATTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑ttttaat                                                                                                                        <  6:861370/149‑5 (MQ=14)

                    acaCCAGATACAAATAAAGAGCCTTGAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑tttta                                                                                                                          <  3:779589/147‑3 (MQ=14)

                     gaCCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGAtt                                                                                                                        <  6:207734/151‑1 (MQ=255)

                     gaCCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑ttttaatt                                                                                                                       <  3:102618/151‑6 (MQ=255)

                     caCCTGAAACAAATAAAGAGTCTTTATGTTGTTAAATAATTGGTTGCGTGGGCCGGATTTGAACCGACGAGCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑ttttaatt                                                                                                                       <  2:395587/150‑6 (MQ=1)

                     caCCTGAAACAAAAAAACACTCTTTAGGTTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑ttttaatt                                                                                                                       <  4:1929360/150‑6 (MQ=14)

                     caCCACAAACACAAAAACCCCCGTTTGGGTGTTAAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑ttttaatt                                                                                                                       <  1:1604520/150‑6 (MQ=14)

                      cccAGCAACAACAAAACCCCGGTGAGGGTGTTCTATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑ttttaattg                                                                                                                      <  1:1387375/150‑7 (MQ=11)

                      accaccaaCAAAAAAACACCCGTTAGGGTGTTAAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑ttttaattg                                                                                                                      <  3:1425766/145‑7 (MQ=14)

                      aCCAGTAACTAAAATACACCCGTTATTGTTTTCAATAATTGGTTGCGTGGGCGGTATTTGAACAGACGACCTTCGGGTTATGATCCCGACGAGCTACCATTCTGCTCCACCACCCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGAtt                                                                                                                        <  6:1692609/150‑1 (MQ=255)

                      aCCAGAAACAAAAACACCCCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑ttttaattg                                                                                                                      <  7:1171006/151‑7 (MQ=14)

                      aCCAGAAACAAAAAAACCCCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑ttttaattg                                                                                                                      <  3:1357560/151‑7 (MQ=255)

                      aCCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTg                                                                                                                       <  7:912998/151‑1 (MQ=255)

                       ccAGAAACAAAAAAAGACCCCTAAGGGTTTTCAATAATTGGTTGCGTGGCCCGGCTTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTgg                                                                                                                      <  7:1280964/151‑1 (MQ=18)

                        cAGAAACAACAAAACACCGGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑ttttaattggt                                                                                                                    <  7:1666638/151‑9 (MQ=14)

                         aGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGtt                                                                                                                    <  3:726734/151‑1 (MQ=255)

                          gAAACAAATAAACAGCTTTTAGGTTGTTAATTTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGtt                                                                                                                    <  4:1384667/150‑1 (MQ=11)

                          gAAACAAAAAAACAGCCGTCAGGGTGTTCAATCATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTg                                                                                                                   <  5:1111919/151‑1 (MQ=11)

                          gAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCTACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTg                                                                                                                   <  6:1290737/151‑1 (MQ=255)

                           aaaCAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCtttac                                                                                                                                          <  3:1717511/128‑3 (MQ=255)

                           aaaCAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCAGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGc                                                                                                                  <  5:1445155/151‑1 (MQ=255)

                            aaCAATAAAACACCCTTTATGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCtttac                                                                                                                                          <  3:1419669/127‑3 (MQ=255)

                            aaCAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCg                                                                                                                 <  3:1612314/151‑1 (MQ=255)

                            aaCAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGACTGGTTGCg                                                                                                                 <  1:1946438/151‑1 (MQ=255)

                             aCAAAAATACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCgg                                                                                                                <  4:1829824/151‑1 (MQ=255)

                              cAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCggg                                                                                                               <  1:169757/151‑1 (MQ=255)

                               caccaaaCACCCGTTTGGGTGTTCAATACTTGGTTGCGGGGGCCGGATTTGACCCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCgggg                                                                                                              <  6:24360/147‑1 (MQ=255)

                                aaaaaaCCCCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCTGATTTGAACCGACGACCTTCTGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCggggg                                                                                                             <  3:1714251/151‑1 (MQ=255)

                                aaaaaaCACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCggggg                                                                                                             <  7:1205555/151‑1 (MQ=255)

                                 aaacaCACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGc                                                                                                            <  2:779770/151‑1 (MQ=255)

                                 aaaaaCACCCGTTAGGGTGTTCAATAATTGGTTGCGGTGGCCGGATTTGAACCGACGACCTTCGTGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTAATTCATCAAA‑TTTGATTGGTTGCGGGGGc                                                                                                            <  8:1497215/151‑1 (MQ=255)

                                                                            gATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAAc                                                                                                 >  5:896293/1‑119 (MQ=255)

                                                                                                        cgcccgcccAGCTTCCAGGCTTCTCCACCCCTCGTCACCGTACTGACTGTACTGCTTCAAA‑ATTTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGt                                     <  5:230770/150‑1 (MQ=11)

                                                                                                                        aGGCGGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATAAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGccc                                                                         >  5:1293932/1‑99 (MQ=255)

                                                                                                                                           cACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACACTAGTCCATAACTTCATCAAATTTTAattgattgagc  >  8:776052/1‑148 (MQ=17)

GAATGGCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGG  >  DH1/936019‑936308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`