Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1936,2930T.28.1% 339.7 / 141.2 139intergenic (‑27/+7)ECDH1_RS04620/ECDH1_RS04625tRNA‑Met/tRNA‑Met
Reads supporting (aligned to +/- strand):  ref base T (92/5);  new base . (37/1);  total (133/6)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.33e-02

Only 100 of 144 total aligned reads displayed.

CACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGATT  >  DH1/936158‑936443
                                                                                                                                        |                                                                                                                                                      
cACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACACTAGTCC‑ATAACTTCATCAAATTTTAattgattgagc                                                                                                                                         >  8:776052/1‑148 (MQ=17)
                                                     aaCCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCCGGAGTCG‑GTGAATTCTACAAAGTATAATTGGTTGCGGCGGGCTTATTTCACCCGCAGGCATTCGGCTTATGAGACCGCCCAGCTACAAgc                                                                                    >  3:1931398/1‑150 (MQ=2)
                                                              ccTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGAGTATTCC‑TTATATTCTCCAATGTGTAATTCCTTGCGGCTGGTTGTTTGCCCCCGACGCCCTTCGGGTTATGATCCCGCCTAGCTAcatccctcctccac                                                                           >  1:58723/1‑138 (MQ=0)
                                                                   gggTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCGGTAGTGC‑GTGAATACAGCAAATTTTAATTCGTTGCGGGGGGCGGATTTGACCCGACGACATTCGGCTTATGAGACCGCCGAGCTACATGGCtcctccccagcgg                                                                      >  5:905235/1‑144 (MQ=9)
                                                                           aGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACGGGAGTCG‑TTGAATTCTGCACTGTGTATTTGGTTGCGGGGTCGGGTTATGACCCGACCGCATTGGGGTTATGTTGCAGACGAGCGACATGGCTCATCCCCCCCTCGTcggggg                                                              >  1:1234510/1‑146 (MQ=2)
                                                                                      cTACCAGGCTGCTCCACCCCGCGTCCCGGTAGGCC‑GTGACTTCATCAAATTTTAATTCGTTGAGCGGGGATTATATGCACCTACCCCATTCGGCTTAAGATACCGACGAACAACCAGCCTCATCCCCACCGGGTCCGGGGATGAGCACtat                                                   >  4:1556253/1‑151 (MQ=255)
                                                                                           aGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTTATTAAATTTTTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGccc                                                                                                  <  6:1295680/99‑1 (MQ=255)
                                                                                           aGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAATTTTTAATTGGTTGCGGGGGCCGGATTTGCACCGACGCCCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGGACTATACTcc                                              >  5:1914265/1‑151 (MQ=255)
                                                                                           aGGCTGCTCCACCCCGCGTCACAGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTCCGGCGGGCTGATTAGAACCGACGACCTTCGGCTTATGAGGCCGACGAGCTACCAGCCTGCTCCACCGATAGTCAGAGCATGAGCACTATACTcc                                              >  6:1955399/1‑151 (MQ=14)
                                                                                             gctgctCCACCCCGCGTCACCGGACTGG‑GTGACTTCATCACATTTTAATTGGTTGCGGGGGCCGGATTAGCAGCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCCCCCCGCGTCAGGGGATGCTCACTATCCTCCGc                                            >  5:989587/1‑151 (MQ=17)
                                                                                               tgctCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCCCCCCGCGTCCGTGGATGCGCACTATACTCCGCTc                                          >  4:1085316/1‑151 (MQ=255)
                                                                                                 ctCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTcgc                                        >  1:253110/1‑151 (MQ=255)
                                                                                                 ctCCACCCCGCGTCACCGTACTGC‑GTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTcgc                                        >  5:148268/1‑151 (MQ=255)
                                                                                                  tCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTcgcg                                       >  3:1035720/1‑151 (MQ=255)
                                                                                                  tCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTcgcg                                       >  6:1818135/1‑151 (MQ=255)
                                                                                                    cACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGtt                                     >  7:542334/1‑151 (MQ=255)
                                                                                                     aCCCCGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCTCTCGCGttt                                    >  6:1031308/1‑151 (MQ=255)
                                                                                                     aCCCCGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGttt                                    >  8:1864206/1‑151 (MQ=255)
                                                                                                       cccGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGCCCCCCTTCGGGTTATGAGCCCGCCGAGCTACCAGGCTGCTACACCCCGCGTCCGGGGAATCCGAATATACTACGCTGGCGTATTc                                  >  6:1630519/1‑151 (MQ=11)
                                                                                                       cccGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTc                                  >  2:975776/1‑151 (MQ=255)
                                                                                                       cccGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTc                                  >  8:885846/1‑151 (MQ=255)
                                                                                                        ccGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCAATATACTACGCTCTCGTTTTc                                  >  3:1409342/1‑150 (MQ=11)
                                                                                                        ccGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGAGGCGGACTATACTCCGCTctatatttc                                  >  3:1572203/1‑142 (MQ=11)
                                                                                                         cgcgTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCCGGGCCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTAACAGGCTGCTCCACCCCGCGTCCCTGGATGCGAACTAAACACCCCACGAGATTTCAt                                >  2:1264727/1‑151 (MQ=11)
                                                                                                         cgcgTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCAt                                >  5:316082/1‑151 (MQ=255)
                                                                                                          gcgTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGTGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATg                               >  6:325057/1‑151 (MQ=255)
                                                                                                           cgTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGGAGTATACTCCGCTGGCGTTTTCAttc                              >  1:321757/1‑149 (MQ=14)
                                                                                                           cgTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCAGGATGCGCACTATACTCCGCTAGAGTTTTCAttg                              >  6:712525/1‑149 (MQ=11)
                                                                                                           cgTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGc                              >  6:868146/1‑151 (MQ=255)
                                                                                                             tCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCa                             >  6:1624235/1‑150 (MQ=255)
                                                                                                              cACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGAGTATGCGGACAATACTACGCTCGATTTTTCATGCAAc                           >  4:745693/1‑151 (MQ=11)
                                                                                                              cACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAAc                           >  2:1298146/1‑151 (MQ=255)
                                                                                                              cACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAAc                           >  7:1408656/1‑151 (MQ=255)
                                                                                                               aCCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGAGGGGCACAATACACCGCTATAGTATTCATTCaagc                          >  1:1356225/1‑149 (MQ=11)
                                                                                                               aCCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGAGGCCCTCTCTACACCCCTGGATTTTTCATGccagc                          >  3:909045/1‑147 (MQ=11)
                                                                                                               aCCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGAGGAGTCGGACTATTCAACGCTCGAGTTttcattcacgc                          >  4:1697793/1‑148 (MQ=11)
                                                                                                               aCCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCTGTATGCCCACAATACTACCAACGCTTTTTCATGCAAcc                          >  4:1505144/1‑151 (MQ=1)
                                                                                                               aCCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGCGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAAcc                          >  1:1612561/1‑151 (MQ=255)
                                                                                                                ccGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCt                         >  3:1732060/1‑151 (MQ=255)
                                                                                                                ccGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCt                         >  4:880341/1‑151 (MQ=255)
                                                                                                                 cGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTAGGGTTAACAGCCCGACGAGCTACCAGGCAGCTCCACCCCGCGTCCCAGTAAGCGATCAATACTCCTAACGCTTTTTCATGCAAccg                         >  5:1182059/1‑149 (MQ=11)
                                                                                                                 cGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtt                        >  1:1061303/1‑151 (MQ=255)
                                                                                                                 cGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtt                        >  2:754537/1‑151 (MQ=255)
                                                                                                                  gTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttt                       >  5:713023/1‑151 (MQ=255)
                                                                                                                   tACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGACCCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTCCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttt                      >  1:231753/1‑151 (MQ=255)
                                                                                                                   tACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttt                      >  5:43240/1‑151 (MQ=255)
                                                                                                                   tACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttt                      >  8:304319/1‑151 (MQ=255)
                                                                                                                   tACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttt                      >  4:656171/1‑151 (MQ=255)
                                                                                                                    aCTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttttt                     >  8:274284/1‑151 (MQ=255)
                                                                                                                    aCTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttttt                     >  1:1749084/1‑151 (MQ=255)
                                                                                                                     ctgcttttACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCAGGATTCGCACAATACTACAATAGAGTTTTCATTCAACCtttttt                    >  2:147563/6‑151 (MQ=11)
                                                                                                                     ctgcttttACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCAGGATGCCCACTATACTCCGATCGAGTATTCATTCAACCtttttt                    >  4:728724/6‑151 (MQ=14)
                                                                                                                     cTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttttt                    >  4:1393421/1‑151 (MQ=255)
                                                                                                                     cTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttttt                    >  1:1558181/1‑151 (MQ=255)
                                                                                                                      tgcttttACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGAGGCGCAGTATTCAGCGCTCTCGTTTTCCTGGaaggtgatttt                   >  7:81371/5‑142 (MQ=1)
                                                                                                                      tGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttttttt                   >  4:1650715/1‑151 (MQ=255)
                                                                                                                      tGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttttttt                   >  2:975462/1‑151 (MQ=255)
                                                                                                                      tGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACACCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttttttt                   >  4:1062712/1‑151 (MQ=255)
                                                                                                                      tGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGCGGCCCGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCCCCTCCGTGGATGCGCACAATACTCAGCTCGAGTTTTCATGCAACCatttttt                   >  2:876035/1‑151 (MQ=21)
                                                                                                                      tGC‑TCTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttttttt                   >  8:1237896/1‑151 (MQ=255)
                                                                                                                       gcttttACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGGACTATACTCCGCTCGCTTATTGCTGCACGCtttttttt                  >  5:470107/4‑151 (MQ=14)
                                                                                                                       gC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttttttt                  >  5:1932510/1‑151 (MQ=255)
                                                                                                                       gC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttttttt                  >  3:1544510/1‑151 (MQ=255)
                                                                                                                         ttttACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCTGGATGCGAAAAATACAACTCAGGAGTTTTCATTCAGGCTTTATTTTa                 >  8:1040933/2‑150 (MQ=11)
                                                                                                                         tttcaCTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGAGGATGCGCAAAATACAACACTCTCTTTTTCATTCAACCttttttttt                 >  2:1896540/5‑149 (MQ=14)
                                                                                                                          tttACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTAc                >  8:443428/1‑151 (MQ=255)
                                                                                                                          tttACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTAc                >  6:401228/1‑151 (MQ=255)
                                                                                                                          tttACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCAGGCACCCTTTTTTTTAc                >  4:442705/1‑151 (MQ=255)
                                                                                                                           ttACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTAcc               >  1:363423/1‑151 (MQ=255)
                                                                                                                           ttACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATTCCATCTATACACCCATGGAGTTTTCATGCAACCTTTTTTTTAccc              >  5:1452940/1‑151 (MQ=14)
                                                                                                                           atACTTCATCAAATTTTAATTGGTTGCCGGGGCCGGATATGAACCGACGACCTACGGGTTATCAGCCAGACGAGCTACCAGGCTGCTCCACGCCGCGTCCGTGGATGCGCACTATACTCCTCTCGCCTTTTCATGCAACCTTTTTTTAAcc               >  6:958737/2‑151 (MQ=21)
                                                                                                                            tACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTAccc              >  8:1536233/1‑151 (MQ=255)
                                                                                                                            tACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGAGGAGGCGCACTATACACCGCTCGCGTTTTCATGCAACCTTTTTTTTAccac             >  5:1717387/1‑149 (MQ=18)
                                                                                                                            tACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGACCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCTGGAGGCGCAAAATACAACAATAGATTTTTCATTCAACCttaataattagac             >  8:1702292/1‑140 (MQ=11)
                                                                                                                             acatcatcaAATTATAAGTGGTTGCGGCGGCCGGATTTGAAACGACGAAATTCGGGATATGACCCAGACGAGCTACCAGGCTGCTCCACCCCGAGTACGTGCATGCGCACAAAAATCCCCTCGCGTTTTCATGCACCCAATTTTTTAccac             >  4:1535609/4‑149 (MQ=12)
                                                                                                                             aCTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGAGGCGGAGTATTCTCCGCTCGCGTTTTCATGCCGCCGTTTTTTTACCCCg            >  7:1785728/1‑151 (MQ=14)
                                                                                                                              cTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCg            >  1:65622/1‑151 (MQ=255)
                                                                                                                              cTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGAGTCGCACTCTACTCCGCAAGAGATTTCATGCATCCTTGGTGTTAGCCCgc           >  6:1842364/1‑150 (MQ=11)
                                                                                                                              cTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGAGGCGCACTATACTCCGCTCGCGTATTCATGCAACCTTTTGTTTACCCCgc           >  5:1878837/1‑150 (MQ=18)
                                                                                                                              cTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCTGGATGCGCAGAATACACCGCTCGCGTTTTCATTGACCCTGATTTTTACCCCgc           >  1:1438271/1‑150 (MQ=14)
                                                                                                                              atttattaaaTTTTTATTGGTTGCGGGGGCCGGATTTGAACCGACGAGCTTCGGGTTATGAGGCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTAcccc             <  6:1490340/143‑1 (MQ=25)
                                                                                                                               ttttccataTTTTACTTTCTTGCGTTGTCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCg            <  8:210740/142‑1 (MQ=25)
                                                                                                                               ttCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCg            >  3:1414050/1‑150 (MQ=255)
                                                                                                                               ttCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCg            >  4:1305936/1‑150 (MQ=255)
                                                                                                                               ttCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCg            >  7:977196/1‑150 (MQ=255)
                                                                                                                               ttCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGAGGCGGAGTATACTCCGCTGGAGTTTTCCTGGAAGCgttgtttttgccagct          >  1:1241381/1‑143 (MQ=11)
                                                                                                                                tcatcaAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCg            <  2:1568435/149‑1 (MQ=255)
                                                                                                                                tcatcaAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGAt          >  7:504423/1‑151 (MQ=255)
                                                                                                                                 tattcaaTTTTTATTGGTTGCGGGGGCCGGATTTGAAGCGACGAGCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGAt          <  2:1363984/145‑1 (MQ=255)
                                                                                                                                 catcaAATTTTAATTGGTTGCGGGGGCCGGATTTGAGCCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCCCCCCGCGTCCGTGGATGCGCACAATACTCCGCTCGCGTTTTCATGCACCCTTTTTTTTACCCCGAt          >  5:1695530/1‑150 (MQ=255)
                                                                                                                                  gtgaaaaTTTCATTGGTTGCGGGGGCCGGATTTGAACGGAGGACCTTGGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCAGCCGGCGTGCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACGTTTTTTTTACCGCGATCa        <  6:1183759/148‑1 (MQ=21)
                                                                                                                                  atcaAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGAGATTTCATGCAACCTTGTTTTTACCCCGATCAc       >  2:441159/1‑151 (MQ=18)
                                                                                                                                   tcaAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttttttt                   >  7:893765/1‑138 (MQ=255)
                                                                                                                                    caAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACg      >  6:1451317/1‑151 (MQ=255)
                                                                                                                                     aaaTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCCCCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACgg     >  1:1203584/1‑151 (MQ=255)
                                                                                                                                     aaaTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACgg     >  4:865456/1‑151 (MQ=255)
                                                                                                                                      aaTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACgg     >  1:1165384/1‑150 (MQ=255)
                                                                                                                                       aTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGAt   >  8:1191417/1‑151 (MQ=255)
                                                                                                                                       aTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGAt   >  3:899212/1‑151 (MQ=255)
                                                                                                                                        ttttAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGAtt  >  3:1738896/1‑151 (MQ=255)
                                                                                                                                        |                                                                                                                                                      
CACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGATT  >  DH1/936158‑936443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑