Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1936,2970AG23.6% 427.1 / 128.9 203intergenic (‑31/+3)ECDH1_RS04620/ECDH1_RS04625tRNA‑Met/tRNA‑Met
Reads supporting (aligned to +/- strand):  ref base A (130/22);  new base G (45/2);  total (175/28)
Fisher's exact test for biased strand distribution p-value = 7.30e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.74e-01

Only 100 of 208 total aligned reads displayed.

CACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGATTTGGC  >  DH1/936158‑936447
                                                                                                                                            |                                                                                                                                                      
                                                                   gggTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCGGTAGTGC‑GTGAATACAGCAAATTTTAATTCGTTGCGGGGGGCGGATTTGACCCGACGACATTCGGCTTATGAGACCGCCGAGCTACATGGCtcctccccagcgg                                                                          >  5:905235/1‑144 (MQ=9)
                                                                                      cTACCAGGCTGCTCCACCCCGCGTCCCGGTAGGCC‑GTGACTTCATCAAATTTTAATTCGTTGAGCGGGGATTATATGCACCTACCCCATTCGGCTTAAGATACCGACGAACAACCAGCCTCATCCCCACCGGGTCCGGGGATGAGCACtat                                                       >  4:1556253/1‑151 (MQ=255)
                                                                                           aGGCTGCTCCACCCCGCGTCACAGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTCCGGCGGGCTGATTAGAACCGACGACCTTCGGCTTATGAGGCCGACGAGCTACCAGCCTGCTCCACCGATAGTCAGAGCATGAGCACTATACTcc                                                  >  6:1955399/1‑151 (MQ=14)
                                                                                                 ctCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTcgc                                            >  1:253110/1‑151 (MQ=255)
                                                                                                   ccACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGt                                          >  8:1186288/1‑151 (MQ=255)
                                                                                                    cACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGtt                                         >  7:542334/1‑151 (MQ=255)
                                                                                                     aCCCCGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGttt                                        >  4:1003495/1‑151 (MQ=255)
                                                                                                     aCCCCGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGttt                                        >  8:1864206/1‑151 (MQ=255)
                                                                                                       cccGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGCCCCCCTTCGGGTTATGAGCCCGCCGAGCTACCAGGCTGCTACACCCCGCGTCCGGGGAATCCGAATATACTACGCTGGCGTATTc                                      >  6:1630519/1‑151 (MQ=11)
                                                                                                       cccGCGTCACCGTACTGCTTTTACTTAATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATTCGCAGTATACTCCtctctatttttc                                      >  2:1460429/1‑143 (MQ=11)
                                                                                                       cccGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTc                                      >  8:885846/1‑151 (MQ=255)
                                                                                                       cccGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTc                                      >  4:1285498/1‑151 (MQ=255)
                                                                                                       cccGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTc                                      >  2:975776/1‑151 (MQ=255)
                                                                                                       cccGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTc                                      >  6:1577797/1‑151 (MQ=255)
                                                                                                        ccGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGAGGCGGACTATACTCCGCTctatatttc                                      >  3:1572203/1‑142 (MQ=11)
                                                                                                        ccGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTc                                      >  7:619682/1‑150 (MQ=255)
                                                                                                         cgcgTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCAt                                    >  5:316082/1‑151 (MQ=255)
                                                                                                          gcgTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATg                                   >  5:996290/1‑151 (MQ=255)
                                                                                                           cgTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGGAGTATACTCCGCTGGCGTTTTCAttc                                  >  1:321757/1‑149 (MQ=14)
                                                                                                           cgTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCAGGATGCGCACTATACTCCGCTAGAGTTTTCAttg                                  >  6:712525/1‑149 (MQ=11)
                                                                                                            gTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGc                                  >  1:844163/1‑150 (MQ=255)
                                                                                                              cACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCCGGATGCGCACTATACTTCGCTCTCGTTTTCATTCAAc                               >  3:1038689/1‑151 (MQ=11)
                                                                                                              cACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAAc                               >  7:1408656/1‑151 (MQ=255)
                                                                                                              cACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAAc                               >  2:567914/1‑151 (MQ=255)
                                                                                                              cACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAAc                               >  2:1298146/1‑151 (MQ=255)
                                                                                                               aCCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGAGGGGCACAATACACCGCTATAGTATTCATTCaagc                              >  1:1356225/1‑149 (MQ=11)
                                                                                                               aCCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGCGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAAcc                              >  1:1612561/1‑151 (MQ=255)
                                                                                                                ccGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCt                             >  4:880341/1‑151 (MQ=255)
                                                                                                                ccGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCt                             >  3:1732060/1‑151 (MQ=255)
                                                                                                                 cGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTAAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGAGGATTCCCAGAATACTGCCCACTCTTTTTCATTCAACCtt                            >  4:332271/1‑151 (MQ=11)
                                                                                                                 cGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtt                            >  6:95497/1‑151 (MQ=255)
                                                                                                                  gTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCGGGAGGCGCACTCTACAGCGCTCGCGTTTTCCTTCAACCgtt                           >  1:1226311/1‑151 (MQ=11)
                                                                                                                  gTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttt                           >  5:713023/1‑151 (MQ=255)
                                                                                                                  gTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGAGCACTATACACCGCTCGCGTTTTCATGCAACCttt                           >  4:768046/1‑151 (MQ=255)
                                                                                                                   tACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttt                          >  4:656171/1‑151 (MQ=255)
                                                                                                                    aCTGC‑TTTACTTCATCAAATTTTAATTGGTTTATGGGGCCGGATTTGAACCTAAGACCTTCTGGTTATGAGCCCTACGAGCTACAAGGCTGCTCAACAACGCGTCCGTTGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttttt                         >  7:1737626/1‑151 (MQ=255)
                                                                                                                    aCTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttttt                         >  1:1749084/1‑151 (MQ=255)
                                                                                                                     cTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttttt                        >  4:1393421/1‑151 (MQ=255)
                                                                                                                      tGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttttttt                       >  4:1650715/1‑151 (MQ=255)
                                                                                                                      tGC‑TCTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttttttt                       >  8:1237896/1‑151 (MQ=255)
                                                                                                                       gcttttACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCAGGAATCGAAATATACAACGCTATATATTTCATTCAACCtttttttt                      >  8:287241/4‑151 (MQ=11)
                                                                                                                       gC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttttttt                      >  5:1932510/1‑151 (MQ=255)
                                                                                                                          tttACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTAc                    >  8:443428/1‑151 (MQ=255)
                                                                                                                          tttACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTAc                    >  6:401228/1‑151 (MQ=255)
                                                                                                                           ttACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATTCCATCTATACACCCATGGAGTTTTCATGCAACCTTTTTTTTAccc                  >  5:1452940/1‑151 (MQ=14)
                                                                                                                           ttACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGTATGCCCAGTATACTCCCATCGAGTATTCATTCAACCTGTATTTTatc                   >  5:1487723/1‑148 (MQ=11)
                                                                                                                            tACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCACCCTTTTTTTTAccc                  >  2:1848981/1‑151 (MQ=255)
                                                                                                                            tACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTAccc                  >  8:1536233/1‑151 (MQ=255)
                                                                                                                            tACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGACCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCTGGAGGCGCAAAATACAACAATAGATTTTTCATTCAACCttaataattagac                 >  8:1702292/1‑140 (MQ=11)
                                                                                                                             aCTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGCCGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTAcccc                 >  6:1444239/1‑151 (MQ=255)
                                                                                                                              cTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCTGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCg                >  1:1487680/1‑151 (MQ=255)
                                                                                                                              cTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCg                >  1:65622/1‑151 (MQ=255)
                                                                                                                              cTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGAGTCGCACTCTACTCCGCAAGAGATTTCATGCATCCTTGGTGTTAGCCCgc               >  6:1842364/1‑150 (MQ=11)
                                                                                                                              cTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCTGGATGCGCAGAATACACCGCTCGCGTTTTCATTGACCCTGATTTTTACCCCgc               >  1:1438271/1‑150 (MQ=14)
                                                                                                                              atttattaaaTTTTTATTGGTTGCGGGGGCCGGATTTGAACCGACGAGCTTCGGGTTATGAGGCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTAcccc                 <  6:1490340/143‑1 (MQ=25)
                                                                                                                               ttttccataTTTTACTTTCTTGCGTTGTCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCg                <  8:210740/142‑1 (MQ=25)
                                                                                                                               ttCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCg                >  4:1491900/1‑150 (MQ=255)
                                                                                                                               ttCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCg                >  7:977196/1‑150 (MQ=255)
                                                                                                                               ttCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGAGGCGGAGTATACTCCGCTGGAGTTTTCCTGGAAGCgttgtttttgccagct              >  1:1241381/1‑143 (MQ=11)
                                                                                                                                agggtgttcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGATGCGCACTATACTc                                                   >  1:180448/12‑114 (MQ=11)
                                                                                                                                 gggtgttgattAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGAt              <  2:1560286/141‑1 (MQ=255)
                                                                                                                                 gggtgatcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATc             <  2:1930207/141‑1 (MQ=255)
                                                                                                                                 catcaAATTTTAATTGGTTGCGGGGGCCGGATTTGAGCCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCCCCCCGCGTCCGTGGATGCGCACAATACTCCGCTCGCGTTTTCATGCACCCTTTTTTTTACCCCGAt              >  5:1695530/1‑150 (MQ=255)
                                                                                                                                 catcaAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATc             >  5:186472/1‑151 (MQ=255)
                                                                                                                                  gtgaaaaTTTCATTGGTTGCGGGGGCCGGATTTGAACGGAGGACCTTGGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCAGCCGGCGTGCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACGTTTTTTTTACCGCGATCa            <  6:1183759/148‑1 (MQ=21)
                                                                                                                                  ggtgttcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGAGTTTTCATGCAACCTTTTTTTTACCCCGATc             >  4:1203124/10‑150 (MQ=255)
                                                                                                                                  atcaAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCAc           <  6:1721165/151‑1 (MQ=255)
                                                                                                                                   tcaAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttttttt                       >  7:893765/1‑138 (MQ=255)
                                                                                                                                    tgttcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCAc                                                          >  2:1025980/8‑103 (MQ=255)
                                                                                                                                    tgttcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCAc                                                          <  1:1021300/96‑1 (MQ=255)
                                                                                                                                    tgttcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGAGTTTTCATGCAACCTTTTTTTTACCCCGATCACg          >  8:1817854/8‑151 (MQ=255)
                                                                                                                                    tgttcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCTGGATTCGAACTATACTCCGCTcg                                             >  4:472422/8‑116 (MQ=14)
                                                                                                                                    caAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCACCCGTTTTTTTACCCCGATCACg          >  3:1508409/1‑151 (MQ=255)
                                                                                                                                     gttcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACg          <  5:1540222/144‑1 (MQ=255)
                                                                                                                                     gttcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTATTTTAccac                 >  7:1270139/7‑141 (MQ=255)
                                                                                                                                     gttcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGAGGATGCGCACTATACTCCGCAAGCGTTTTCATGCAACCTTATTTTTa                     >  4:511024/7‑139 (MQ=18)
                                                                                                                                     gatcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTa                     <  3:506633/133‑1 (MQ=255)
                                                                                                                                     gatcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACgg         <  5:1888723/145‑1 (MQ=255)
                                                                                                                                     caaatttGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCCTCACTGGAAGCCCACTATACTACACTCTATATTTCATGCAACCTTAATTATAccaacatacacc         >  2:826947/5‑141 (MQ=2)
                                                                                                                                      aaatttGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGTATGCGCACTATACTCCAATAGAGTTTTCATGCAACCTTTATTTTAACCCGATAACgc         >  6:95981/4‑149 (MQ=17)
                                                                                                                                      aaatttGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGAGGAATCGCACTATACTCCGCTAGCGTTTTCATGCATCCTTTTTTTTAACACGATCACgg         >  6:34932/4‑150 (MQ=18)
                                                                                                                                      aaTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACgg         >  1:1165384/1‑150 (MQ=255)
                                                                                                                                       tcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGATCGATTTTTCATg                                   >  2:1749483/5‑123 (MQ=255)
                                                                                                                                       aTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGAt       >  5:462020/1‑151 (MQ=255)
                                                                                                                                       aTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGAt       >  3:899212/1‑151 (MQ=255)
                                                                                                                                        ttttAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGCCGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGAtt      >  2:952394/1‑151 (MQ=255)
                                                                                                                                        ttttAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGAtt      >  4:709301/1‑151 (MQ=255)
                                                                                                                                        caatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTc                                              >  3:1217615/4‑111 (MQ=255)
                                                                                                                                        atttGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGAGGCGCACTATACTCCGCTCTCTTTTTGCTGCAGGCTGGTGTTTAGCCCGAGCACGGCtt      >  3:1411649/2‑151 (MQ=14)
                                                                                                                                        atttGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCAGAGGAAGCGCACTATTCTACGATAGAGATTTCATTCAACCTTATTTATAGCCCGATCACGCAtt      >  6:1050141/2‑151 (MQ=14)
                                                                                                                                        atttGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTCCTCCACCCAGCGTCCGTGGATTCCCACAATACTACACTCTATATTTCATTCAACCTTTATTATAACAAGATCACTGAtt      >  8:545803/2‑151 (MQ=9)
                                                                                                                                         tttAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGGTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGAttt     >  8:1309077/1‑151 (MQ=255)
                                                                                                                                         tttAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGAttt     >  3:1808329/1‑151 (MQ=255)
                                                                                                                                         tttAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTCGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGAttt     >  4:1527359/1‑151 (MQ=255)
                                                                                                                                         aatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATTCGCAGTATACTCCGCTCGCGTTTTCCTTCACGCTTGTTTTTACCCCGATCACGGAttg     >  3:1933987/3‑150 (MQ=18)
                                                                                                                                         aatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGGAGTATACTCCGCTCGCGTTTTCATGCACCCTGTTTTTTACCCCGATCCCGCAttt     >  1:404169/3‑151 (MQ=18)
                                                                                                                                         aatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGAttt     <  2:112474/149‑1 (MQ=255)
                                                                                                                                          ttAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGATTTg    >  7:495590/1‑151 (MQ=255)
                                                                                                                                          atAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGATTTg    <  5:1333824/150‑1 (MQ=255)
                                                                                                                                            aaTTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGATTTGGc  >  5:1143434/1‑151 (MQ=255)
                                                                                                                                            |                                                                                                                                                      
CACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGATTTGGC  >  DH1/936158‑936447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑