Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH11,065,6050AT24.7% 307.3 / 101.2 194intergenic (+40/‑23)ECDH1_RS05245/ECDH1_RS05250tRNA‑Arg/tRNA‑Arg
Reads supporting (aligned to +/- strand):  ref base A (91/55);  new base T (38/10);  total (129/65)
Fisher's exact test for biased strand distribution p-value = 3.52e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.79e-01

Only 100 of 211 total aligned reads displayed.

ATCAAAAAATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGC‑GGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTAT‑C‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTAA  >  DH1/1065455‑1065755
                                                                                                                                                            |                                                                                                                                                        
    aaaacTCTGCAGTAATCTAACGTTCGCGATGCACAGGTAGCTCAGCGGGCGAGACTAATCGGCTACCACGGGTAG‑GGGCGCAGGTTGTAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTAT‑C‑‑AAT‑‑ATcagcag                                                                                                                                                      <  7:1519497/151‑1 (MQ=9)
        aTCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGC‑GGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTAT‑C‑‑AAT‑‑ATca                                                                                                                                                          >  1:1342361/1‑143 (MQ=255)
          cTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGC‑GGTCGGAGGTTCGAATCCTCCCGGATGCACCATATGGTACTATATATTCAGGTAGTAGCGGAAC‑C‑‑AAG‑‑ATCAACAGGaaaa                                                                                                                                                 >  3:108807/1‑150 (MQ=11)
            gCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGC‑GGTCGGAGGTTCGAATCCTCCCGGATGCACCATATATTACTAGATATGGCTATAGTAGCGGTAT‑C‑‑AAT‑‑ATCAGCAGTAaatta                                                                                                                                               >  3:1296271/1‑150 (MQ=255)
             cAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGC‑GGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTAT‑C‑‑AAT‑‑ATCAGCAGTAaaataa‑a                                                                                                                                            <  1:889574/151‑1 (MQ=255)
              agtaaagtaaGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGC‑GGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTGTACGTGATATGGCGGAAGGAGGGGTTG‑C‑‑AAT‑‑ATCAGGCGTAaaataa‑at                                                                                                                                           >  7:746749/1‑151 (MQ=14)
                  aagtaagtTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGC‑GGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTGTTACTAGATATGGCTTTAGTAGCGGTAT‑C‑‑AAT‑‑ATCAGCAGTAAAATAA‑Atttgc                                                                                                                                       >  4:650139/1‑149 (MQ=255)
                   tgcgatctgccatttGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGC‑GGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTAT‑C‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTcc                                                                                                                                       <  5:96936/136‑1 (MQ=255)
                   ccctctccgccatttGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGC‑GGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTAT‑C‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTccc                                                                                                                                      <  1:115454/137‑1 (MQ=255)
                    gtaagtTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGC‑GGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTAT‑C‑‑AAT‑‑ATCAGCAGTAAAATAG‑Atttga                                                                                                                                       >  5:582110/1‑147 (MQ=255)
                      acacagccatttGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGC‑GGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTAT‑C‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGAt                                                                                                                                   <  5:1320979/140‑1 (MQ=255)
                       agtTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGC‑GGTCGGAGGTTCGAATCCTCCCGGATGCACCATATAATACTAAATATTCATATATTAAC‑GTATAC‑‑AAT‑‑ATCAACAGTAaaataa‑ataacccaat                                                                                                                                   >  2:449762/1‑142 (MQ=11)
                          ttcgccatgacgccGTAGCACACCTGTATATAGGAATCGGCTACTAACGCTGC‑GGTCTTTGGGTGTAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTAT‑C‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCa                                                                                                                                <  7:81659/138‑1 (MQ=9)
                                ttGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGC‑GGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTAT‑C‑‑AAT‑‑ATcagcag                                                                                                                                                      <  3:420582/122‑1 (MQ=255)
                                   cATCCGTAGCTCAGCTGCATAGACTACGCGGCTACGAACCCCGC‑GGTCGGATTTTATAATCCTCCCGGCAGCACCATCTCTTACTTTATATGTATTTATTAGCGGTAT‑C‑‑AAT‑‑ATCAGCAGTAAATTAA‑ATTTCCCTATGCATCCGTAGCTc                                                                                                                      <  6:519725/151‑1 (MQ=18)
                                    agccGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGC‑GGTCGCAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTAT‑C‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCa                                                                                                                     <  8:1094829/149‑1 (MQ=255)
                                        gTAGCTCAGCTTTTTATTCTACTCGGCGACGACCGCAGC‑GCTCCTCGGGTGGTATGCTCCCTGATGCACCATCTCTTACTTGATCTGGCTTTAGTCGCGCTCT‑G‑‑AAT‑‑ATCACCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTg                                                                                                                 <  7:866746/151‑1 (MQ=9)
                                        gTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGC‑GGTCGGAGGTTCGAATCCTCCCGGATTCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTAT‑C‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTg                                                                                                                 <  6:218085/151‑1 (MQ=255)
                                        gTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGC‑GGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTAT‑C‑‑AAT‑‑ATCAGCAGTAAAATAACTTTTCCCCATGCaagctaggaatagct                                                                                                                  >  2:490764/1‑137 (MQ=21)
                                          aGCTCAGGTGGATAGAGAACTCGGCGA‑GGACCGCGCGGGTCGGCGCGTCTAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGCTAT‑C‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGa                                                                                                               <  7:1184536/151‑1 (MQ=17)
                                             tCAGCTGGATAGAGTACTCGGCTACGAACCGAGC‑GGTCGGAGGTTCGAATCCTCCCGGATGCACCATATATTACTTGATATTGCTGTAGTAGCGGTTT‑C‑‑AAT‑‑ATCAGCATTAAAATAA‑ATTTCACGATGCATACGTAGCTCAGCTGGATat                                                                                                            >  8:1423560/1‑150 (MQ=21)
                                               agctgttttttgTACTCGGCGACG‑ATCGAGCGGGTCGGCGCGTCTAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTAT‑C‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATagag                                                                                                          <  3:473748/140‑1 (MQ=14)
                                                gCTGGTTAGTCTACTCGGCTACGAACGGGAC‑GCGCGGCGCGTGGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTAT‑C‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGt                                                                                                         <  7:1364356/151‑1 (MQ=14)
                                                      tAGAGTACTCGGCTACGAACCGAGC‑GGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTAT‑C‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTACCGATTCATCCGTAGCGCATGTGGTTagaatcatcgg                                                                                                   >  1:823862/1‑143 (MQ=21)
                                                         agTACTCGGCTACGAAGCGCGC‑GGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTTGTTTATTTTTTTTTAGTCGGTGTAT‑C‑‑AAT‑‑ATCTGCAGTAAAATAA‑ATTTCCCGATGCATCAGTAGCTCAGCTGGATAGAGTACTCGGCTa                                                                                                <  4:122069/151‑1 (MQ=18)
                                                              tCGGCTACGAACCGAGC‑GGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCGTACTTGATATGGCTGTATGAGCGGTAG‑C‑‑AAT‑‑ATCTGGAGTAAAATAA‑ATTTCCCGCTGCATCGGTAGAACATCTGGATAGAGTACGCGGCTCCGagc                                                                                           >  7:496386/1‑149 (MQ=18)
                                                                   tACGAACCGAGC‑GGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTAGTACTTGATATGGCGGTAGGAGGGGTTG‑G‑‑AAT‑‑ATCTGCAGTAATTTAA‑ATTTCCCGATGCCTCGGTAGCTCATCTGGATAGAGTCGTCGGCTACgcccccagc                                                                                      >  1:1040411/1‑143 (MQ=19)
                                                                   gacgacgggggC‑GGTCTGAGCGTCTAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTAT‑C‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGc                                                                                     <  8:87492/141‑1 (MQ=14)
                                                                   caCGAACCCCGC‑GGTCGGCGGGTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTATTAGCTTTAT‑C‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGc                                                                                     <  5:660504/150‑1 (MQ=17)
                                                                      gAACCGAGC‑GGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTAT‑C‑‑AAT‑‑ATCAGCAGTATAATAAGTTTTCCCGATGCAt                                                                                                                               >  7:1447086/1‑108 (MQ=14)
                                                                      gAACCGAGC‑GGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTAT‑C‑‑AAT‑‑ATCAGCAGTAGAATAAGTTTTCCCGATGCAACCTTAg                                                                                                                         >  2:1720464/1‑114 (MQ=14)
                                                                       aaCCGAGC‑GGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTAT‑C‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTGCCGATGCATCCGTAGCTGAGCTGGATAGAGTACTCGGCTACGAAC‑Cgcgccggg                                                                                 >  7:1266615/1‑147 (MQ=255)
                                                                                ggTCGGAGGTTCGAATCCTCCCGGATGCACCATATGGTACTTGATATTGCTGTAGGAGCGGTAG‑G‑‑AAT‑‑ATGAGGAGTAAATTAA‑AGGACGCGATAGGTAGGATGAACAGCGGTAAAGAGAAGTCCGCTACGAGC‑CGAGCGGGCGGAGGt                                                                           >  4:1857279/1‑149 (MQ=9)
                                                                                    ggagcgtctaagcctccCGGATGCACCATCTCTTACTTGATCTGGCTTTACTAGCGGTAT‑A‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAAt                                                                     <  5:1339579/139‑1 (MQ=14)
                                                                                     gcgcgtgtaaTCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTAT‑C‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAAtc                                                                    <  3:1834000/143‑1 (MQ=255)
                                                                                     gcgagtataaTCCTCGCTGTTTCACGATCTCTTACTTTATTTGGTTTTATTAGGTTTAT‑A‑‑AAT‑‑ATCAGCAGTAAAATAA‑CTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAAt                                                                     <  2:294584/142‑1 (MQ=11)
                                                                                     gAGGTTCGAATCCTCCCGGATGCACCATATGGTACGAGATATGCCTAAAGTACCGGAAA‑C‑‑AAG‑‑ATCTCCAGTAAAAAAA‑ATAACCCCATGCATAAGAAGCAGAGCTGGATAGACAACACCGCTACGCAC‑CCAGCCAGCGGAGGGTCGaaac                                                                    >  4:1816420/1‑149 (MQ=255)
                                                                                       gggtataaTGCTCGCGGATGCACCATCTCTTAGTTGATATGGCTTTAGTAGGTGTAT‑A‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAAtcct                                                                  <  7:56084/145‑1 (MQ=11)
                                                                                         ttCGAATCCTCCCGGATGCACCATCTATAACTTAATATAGAATTATAAGCAAAAA‑C‑‑AAT‑‑AAAAAAATTAATATAA‑ATATCACGATGCATAAGAATAAAAAAAAAATAGAGAACTCGGCTACGAAC‑CGAGCGTTCGGAgtaaccaaacctgc                                                                >  8:1570653/1‑138 (MQ=255)
                                                                                             aaTCCTCCCTGATTCACCATCTCTTACTTTATATGGCTTTTTTAGCTTTAT‑A‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTccc                                                               <  4:1086124/148‑1 (MQ=14)
                                                                                              agcctccCGGACGCACCATCTCTTACTTTATCTGGCTTTAGTAGGGCTAT‑C‑‑AAT‑‑ATCAGCAGTAAACTAA‑CTTTCCCGATGCAGCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGAt                                                           <  1:550248/149‑1 (MQ=11)
                                                                                              aTCCTCCCGGATGCACCATCTGTTACTTGATATGGCTTTATTAGCGGTAG‑C‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATAAGTAGCTCAGCTGGATAGAGTACTCGGCTACGAGC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGAt                                                           >  7:195774/1‑151 (MQ=14)
                                                                                               tcctccCGGATGCACCATCTCTTACTTGATAAGGCTTTAGTAGCGGTAT‑C‑‑AAT‑‑ATCAGGAGTaa                                                                                                                                                   >  2:1056749/1‑64 (MQ=255)
                                                                                                ccgcccGGATGCACCATCTCTTCCTTGATATGGCTGTAGAAGCGCTAT‑C‑‑CAT‑‑ATCTGCAGTCACATAA‑CAATCCCGCTGTACCGCTATCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGc                                                         <  7:969943/148‑1 (MQ=11)
                                                                                                    ccGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTAT‑C‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCAcc                                                      >  8:855849/1‑150 (MQ=40)
                                                                                                     cggccgCACCAGCTCATACTTGATATCGCTTTAGTAGCTTTAT‑C‑‑AAA‑‑ATCAGCAGGAAAATAA‑CATTCCCGCAGCATCCGGAGCTCAGCTGGAGAGAGCACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCat                                                    <  3:649107/146‑1 (MQ=11)
                                                                                                           cACCATCTCTTACTTGATATGGCTTTAGTAGCGGTAT‑C‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATtctc                                              >  2:301241/1‑151 (MQ=39)
                                                                                                           cACCATCTCTTACTTGATATGGCTTTAGTAGCGGTAT‑C‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTACCGATGCATCCCTAACTCAGCTGGATAGAGTACTACGCTACGAAC‑CGAGCGGTACGAGGTTCGAATCATCCCCCATGCACCAAATtct                                               >  2:1688837/1‑150 (MQ=14)
                                                                                                               aTCTCTTACTTGATATGGCTTTAGTAGCGGTAT‑C‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGa                                           >  1:1125138/1‑150 (MQ=40)
                                                                                                                  tctTACTTGATATGGCTTTAGTAGCGGTAA‑A‑‑AAT‑‑ATCAGCATAAAATTAA‑ATTTCACTATTAATAATTAGGTAAGATGGATAGAGTACTCGGATAATAAA‑AGAAAGGTCGGAGGTTCGAATCCTCCCGGCTGCACGATATTGTCCGAAAtg                                       >  6:1551198/1‑150 (MQ=9)
                                                                                                                   tcctatctatagtgCTTTATTATAGGTAT‑C‑‑AAAAAATCTGCAGTAAACTAA‑CTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGaaa                                         <  4:1704033/137‑1 (MQ=11)
                                                                                                                     tACTTGATATGGCTTTAGTAGCGGTAT‑C‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTc                                    >  2:78114/1‑151 (MQ=255)
                                                                                                                        ttGATACGGCTTTAGTAGCGGTAT‑CAAAAA‑‑ATCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCCGATGCACCATATTATACGAAAtatac                                    >  2:1182369/1‑146 (MQ=14)
                                                                                                                          gATATGGCTTTAGTAGCGGTAT‑C‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGTAAACTTCAGCa                                >  5:1970077/1‑150 (MQ=25)
                                                                                                                          gATATGGCTTTAGTAGCGGTAT‑C‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCa                                >  5:1496651/1‑150 (MQ=255)
                                                                                                                          gATATGGCTTTAGTAGCGGTAT‑C‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATACGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCCGATGCACCATATTATCCGtaaacagaaccg                                >  2:218738/1‑141 (MQ=11)
                                                                                                                          gATACGGCTTTAGTAGCGGTAT‑CAAAAA‑‑ATCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTATCCTAAATATTCatt                                 >  6:1041623/1‑149 (MQ=14)
                                                                                                                           atacGGCTTTAGTAGCGGTAT‑CAAAAA‑‑ATCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTACGaaaacatcgga                                 >  3:1694510/1‑142 (MQ=14)
                                                                                                                             atGGCTTTAGTAGCGGTAT‑C‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATg                             >  3:229851/1‑150 (MQ=255)
                                                                                                                             atGGCTTTAGTAGCGGTAT‑C‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAAGCTTCAGCAATg                             >  1:854482/1‑150 (MQ=255)
                                                                                                                              tGGCTTTGGTAGCGGTAT‑C‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGa                            >  4:728878/1‑150 (MQ=255)
                                                                                                                              tGGCTTTAGTAGCGGTAT‑C‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGTAAACTTCAGCAATGa                            >  5:688247/1‑150 (MQ=25)
                                                                                                                              cggCTTTAGTAGCGGTAT‑CAAAAA‑‑ATCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTATCCGAAATATTCAGCAAtt                             >  3:180868/2‑150 (MQ=11)
                                                                                                                               ggCTTTAGTAGCGGTAT‑CAAAAA‑‑ATCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATACTACTAAATATTCataaatt                             >  8:1249305/1‑144 (MQ=14)
                                                                                                                               ggCTTTAGTAGCGGTAT‑C‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGACTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGa                            >  7:1552733/1‑149 (MQ=255)
                                                                                                                                gcttttttagctctct‑C‑‑AAT‑‑AGCACCACGAAAATAA‑TTTTCCCGCTGCCTCCGTATCACCGCTGGTAAGAGTACTCGGCGACCACCGCTAGCCG‑CCACGGGAGTAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAgg                         <  7:59898/136‑1 (MQ=2)
                                                                                                                                gCTTTAGTAGCGGTAT‑CAAAAA‑‑ATCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATAATACTAAATATTCAGAAATGa                            >  2:1318599/1‑150 (MQ=14)
                                                                                                                                 cTTTAGTAGCGGTAT‑C‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGt                        >  6:1054680/1‑151 (MQ=255)
                                                                                                                                   ttAGTAGCGGTAT‑CAAAAA‑‑ATCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTACGATATCTTGAGCTATGAACGt                        >  1:164333/1‑151 (MQ=11)
                                                                                                                                      gtagCGGTAT‑CAAAAA‑‑ATCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATACTCCGAAATCTTCCTCAATGaacggat                      >  8:510971/1‑145 (MQ=11)
                                                                                                                                       tagCGGTAT‑CAAAAA‑‑ATCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATAATACGATATCTGCAGAAAttagcggaacc                    >  1:1245506/1‑141 (MQ=14)
                                                                                                                                        agCGGTAT‑CAAAAA‑‑ATCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCATCCCGGATGCACCATATTATACTAAATATTCATAAATGatcgtaagg                    >  4:1914853/1‑142 (MQ=14)
                                                                                                                                        agCGGTAT‑C‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGTAAACTTCAGCAATGAAGGTATCGaa                  >  8:1778317/1‑150 (MQ=25)
                                                                                                                                        agCGGTAT‑A‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGTAACCTTCAGCAATGAAg                          <  3:1053043/142‑1 (MQ=16)
                                                                                                                                           tgggttataatt‑‑atCTGCATTAAATTAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGTAACCTTCAGCAATGAAGGTATCGaa                  <  6:689167/139‑1 (MQ=11)
                                                                                                                                           ggTAT‑C‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGTAAGCTTCAGCAATGAAGGTATCGAAGAgg              >  1:1594134/1‑151 (MQ=25)
                                                                                                                                            ccgagc‑‑AAA‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGTAACCTTCAGCAATGAAGGTATCGAagag               <  7:1834917/145‑1 (MQ=255)
                                                                                                                                             tAT‑C‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGAATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAA‑CGATCGGTCGGAGGTTCGAATCCTCCCGGATTCACCATATTATCCGAAAACTTCAGCAATGAAGGTATCGAagagat             >  6:1325225/1‑148 (MQ=14)
                                                                                                                                              tt‑C‑‑ATT‑‑ATCTGCAGACAAATAT‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCgta                                          <  3:1931359/119‑3 (MQ=11)
                                                                                                                                              ggtatcaaaaaatCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTGTACTAAATATTCAGAAATGAAGGTATGGa                   >  1:1284704/12‑148 (MQ=11)
                                                                                                                                              aT‑C‑‑AAT‑‑ATCAGCAGTAAAATAA‑GTTTCCCGATGCAGCCGTAGCTCAGCTGGATAGAGTACTCGGGTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTa            <  3:1228450/150‑1 (MQ=255)
                                                                                                                                               t‑C‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGTAACCTTCAGCa                                <  6:1023035/129‑1 (MQ=18)
                                                                                                                                                tatcaaaaaatCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTACGAAATCTTCAGCAATGAAGGTAACGAATAgg              >  5:1169002/10‑151 (MQ=11)
                                                                                                                                                 c‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAAt                                                                     >  5:748375/1‑91 (MQ=255)
                                                                                                                                                  aaaaT‑‑ATCTGCAGTAAATTAA‑TTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGc          <  4:507644/149‑1 (MQ=255)
                                                                                                                                                    aaT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAgg                         <  8:1951714/134‑1 (MQ=255)
                                                                                                                                                    aaT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGCTAGCgg        >  1:1703306/1‑151 (MQ=255)
                                                                                                                                                     at‑‑atCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAAACTTCAGCAATGAAGGTATCGAagag               >  8:1838534/1‑143 (MQ=255)
                                                                                                                                                     at‑‑atCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACAATATTCTCCGTAAACTTCAGCAATGAAGGTaagataggaatagcgt        >  4:1334170/1‑135 (MQ=11)
                                                                                                                                                     aaaatccagcagAAAAAAAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGAt                                                           <  1:794087/95‑1 (MQ=14)
                                                                                                                                                     aaaaatCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTGTACGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAg           >  3:981636/5‑149 (MQ=14)
                                                                                                                                                      t‑‑atCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGTAACCTTCAGCAATGAAGGTATCGAAGAGATAGc          <  3:135294/147‑1 (MQ=9)
                                                                                                                                                        aatCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTGTCCGACATATTCAGGAATGAACGGAAGCAAGAGGTAGAGGa       >  6:1194643/2‑150 (MQ=11)
                                                                                                                                                         atCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGt                                                                                  <  1:461944/74‑1 (MQ=255)
                                                                                                                                                         atCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAAtcct                                                                  >  1:1852144/1‑90 (MQ=40)
                                                                                                                                                         atCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAAt     >  2:797351/1‑151 (MQ=255)
                                                                                                                                                           cagcagTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGATGTAGAGGAAtt    >  6:1710533/1‑150 (MQ=255)
                                                                                                                                                           cagcagTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAAtt    >  3:1445351/1‑150 (MQ=255)
                                                                                                                                                            agcagTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTaa  <  5:1040143/151‑1 (MQ=255)
                                                                                                                                                            agcagTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTa   >  5:1717194/1‑150 (MQ=255)
                                                                                                                                                            |                                                                                                                                                        
ATCAAAAAATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGC‑GGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTAT‑C‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTAA  >  DH1/1065455‑1065755

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑