BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	DH1	2,326,287	0	G	T	28.1%	761.1 / 92.6	404	intergenic (+207/+200)	yddM/fdnI	transcriptional regulator/formate dehydrogenase nitrate‑inducible cytochrome b556(Fdn) subunit
Reads supporting (aligned to +/- strand): ref base G (200/81); new base T (30/80); total (234/170)
Fisher's exact test for biased strand distribution p-value = 3.19e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 406 total aligned reads displayed.

ATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCT‑ACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATG  >  DH1/2326138‑2326437

aTGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCT‑ACAATATGAAAATGACGCCTTTTACAGCGCCATTTGa                                                                                                                                                       <  5:604713/151‑1 (MQ=33)

  gcgcTACGCTTATCAGTCCTACACGTTCACTCCAATCTTTTGGATTTGAAGGGTTTTCATGCCGGATAAGTCTTTAACGCCGCATCGGGCATGAACAAAGCCTAGGTTTTCT‑ACAATATGAAAATGACGCCTTTTACAGCGCCATTTGaaa                                                                                                                                                     <  1:1635421/151‑1 (MQ=11)

  gcgcTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCT‑ACAATATGAAAATGACGCCTTTTACAGCGCCATTTTaa                                                                                                                                                      >  8:1446447/1‑150 (MQ=14)

  gcgcTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCT‑ACAATATGAAAATGACGCCTTTTACAGCGCCATTTGaaa                                                                                                                                                     <  7:347525/151‑1 (MQ=34)

      tACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCT‑ACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACtg                                                                                                                                                  >  2:2497/1‑150 (MQ=37)

       aCGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCAGATAAGGCGTTCACGCCTCAGCCGTCAAGACAAAGGACTAGGTTGGGT‑AGAAGATGAAAATGAGGAAAATTAAAGCGCCATGTGagagtgct                                                                                                                                                >  5:705032/1‑144 (MQ=2)

        cGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCT‑ACAATATGAAAATGACGCCTTATAAAGCAACATATTAAACtaatg                                                                                                                                               >  6:1275613/1‑151 (MQ=11)

         gCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCT‑ACAATATGAAAATGCCGCCTTTTACAGCGCCATTTGAAACtgatg                                                                                                                                               >  8:655038/1‑150 (MQ=37)

         gCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCT‑ACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAACCtgaagc                                                                                                                                              >  3:1411223/1‑148 (MQ=25)

         gCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCT‑ACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACtgatga                                                                                                                                              <  3:1684869/151‑1 (MQ=39)

         gCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCT‑ACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACtgatga                                                                                                                                              <  1:830432/151‑1 (MQ=39)

                aGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATACGGCATGAACAAAGCCTACGTTGTCT‑ACAATATGAAAATGACGCCTTTTACAGAGGAATTTGAAACTGAAGACAAAAGc                                                                                                                                       >  6:350825/1‑151 (MQ=14)

                      acacGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCT‑ACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACa                                                                                                                                   <  1:304294/149‑1 (MQ=255)

                       aaagTTCACTTCAATCTATTGTATTTGAATGGTTTTTATGCCGGATTAGGGGTTCACGCGGGTTGCGGGATGTACAAAGCCTTCGTTGTCT‑ACAATATTAAAATGACTCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTg                                                                                                                                <  6:1624464/148‑1 (MQ=255)

                             aaCTGCAATGTATTGTATTTGAAGGGTTTTCATGCCGGATAAGGCTTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCT‑ACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGAt                                                                                                                          <  6:1734393/150‑1 (MQ=255)

                                   aaTCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGTCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCT‑ACAATATGAAAATGACGCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTa                                                                                                                    <  2:1616171/151‑1 (MQ=255)

                                    aTCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCT‑ACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCACCATTGCCTGATGCGCTAc                                                                                                                   >  3:1214411/1‑151 (MQ=255)

                                    aTCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAACCCTACGTTGTCT‑ACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGACTGATgcgatcc                                                                                                                   >  8:261684/1‑147 (MQ=255)

                                        aTTGGTTTTGAAGGGTTTTCATGCCGTTTAAGGCTTTCACGCCGCATCCTGCATGAACAAAGCCTACGTTGTCT‑ACAATATGAAAATGACGCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCt                                                                                                                <  2:895532/150‑1 (MQ=18)

                                        aTTGGATTTGAAGGGTTTTCATGCCGGATAAGGGGTTCACGCCGGATCCTGCATGAACAAAGCCTACGTTTTCT‑ACAATATTAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGc                                                                                                                 <  7:1900045/149‑1 (MQ=255)

                                          tGGATTTGAAGGGTGTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCT‑ACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAGCATTGCCTGATGCGCTACGCTTa                                                                                                              <  1:1912247/150‑1 (MQ=255)

                                             aTTTGAAGGGTTTTCATGCCGGATAAGCCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCT‑ACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCa                                                                                                           <  5:95905/150‑1 (MQ=255)

                                              tttGAAGGGTTTTCATGCCGTATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCT‑ACAATATGAAAATGACGCCTTTTAAAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAg                                                                                                          <  6:1058549/150‑1 (MQ=255)

                                                 gAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCT‑ACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGcc                                                                                                       <  1:1325215/150‑1 (MQ=255)

                                                  aaGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCT‑ACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTa                                                                                                     <  1:1429048/151‑1 (MQ=255)

                                                   aGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCT‑ACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTa                                                                                                     >  4:1887373/1‑150 (MQ=255)

                                                     ggTTTTCATGCCGTATAAGTCTTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCT‑ACAATATTAAAATGACGCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTacac                                                                                                  <  4:75870/151‑1 (MQ=255)

                                                      gTTTTCATGCCGGATAAGGCTATCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCT‑ACAATATTAAAATGACTCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACg                                                                                                 <  1:1532908/151‑1 (MQ=255)

                                                      gTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCT‑ACAATATGAAAATGCCGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACg                                                                                                 >  5:1440422/1‑151 (MQ=255)

                                                      gTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCT‑ACAATATGAAAATGACGCCTTTTACAGAGCCATTTCAAACTGATCAGAAACACAACATTCACTGATGCGCTACGATTATCATGAATacac                                                                                                  >  4:1620593/1‑150 (MQ=17)

                                                        tttCATGCCTGATTATTATTTCACGGCGCATCCGGCATTAACAAAGAATACTTTTTCT‑ACAATATGAAAATGACGCGTTTTACAGCTCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGt                                                                                                <  4:1525388/150‑1 (MQ=18)

                                                         ttCATGCCGTTTAAGGCTTTCACGCCGCATCCGGAATGAACAAAGCCTACTTTGTCT‑ACAATATTAAAATGACGCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGtt                                                                                               <  8:1191080/150‑1 (MQ=25)

                                                          tCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCT‑ACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTc                                                                                              >  8:558884/1‑150 (MQ=255)

                                                           cATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCT‑ACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCAc                                                                                            >  7:61867/1‑151 (MQ=255)

                                                             tGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCT‑ACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTg                                                                                          >  3:1297266/1‑151 (MQ=255)

                                                              gCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCT‑ACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGc                                                                                         >  4:294182/1‑151 (MQ=255)

                                                              gCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCT‑ACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGc                                                                                         >  7:1491236/1‑151 (MQ=255)

                                                                cGGATAAGGCGTTCACGCTGCATCCGGCATGAACAAAGCCTACGTTGTCT‑ACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCa                                                                                        >  2:606298/1‑150 (MQ=255)

                                                                cGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCT‑ACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCa                                                                                        >  7:884957/1‑150 (MQ=255)

                                                                cGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCT‑ACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGAAACATTGCCTGATGCGATACGCTTATCAGTCCTACACGTTCACTGCa                                                                                        >  4:901134/1‑150 (MQ=255)

                                                                  gATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCT‑ACAATATGAAAATGACGCCTTTTACAGCGCCATTTTAAACTGATGAAAAACTCAACATTGCCTGATTCGCTACGCATATCAGGCCTACACGTTCACTGCAAt                                                                                      >  6:403351/1‑150 (MQ=255)

                                                                   aTAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCT‑ACAATATGAAAATGACGCCTTTTACAGCGCCATTTTAAACTGATGACACAAGCAACATTGCCTGATTCGCTACGCTTATCAGTCCTACACGTTCACTGCAATCt                                                                                    >  4:1121515/1‑151 (MQ=255)

                                                                   aTAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCT‑ACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCt                                                                                    >  8:1870171/1‑151 (MQ=255)

                                                                     aaGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCT‑ACAATATGAAAATGACGCCTGTTACAGCGCCATTTGAAACTGATGACACACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCACTCTAt                                                                                  >  5:755256/1‑151 (MQ=255)

                                                                      gggCTTTCACGCCGCATCCGGGATGTACAAAGCCTACGTGTTCT‑ACAATATCAAAATGTCGCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTAtt                                                                                 <  1:610541/150‑1 (MQ=21)

                                                                      aGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCT‑ACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAAAGCAACATTGCCTGATGCGCTACGCTTATCAGTCCTACACGTTCACTGCAATCTAtt                                                                                 >  6:866349/1‑151 (MQ=255)

                                                                       ggCGTTCACGCCGCATCCTGCATGAACAAAGCCTACTTTGTCT‑ACAATATGAAAATGACTCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTg                                                                                <  1:423518/151‑1 (MQ=255)

                                                                       ggCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTAT‑ACAATATTAAAATGACGCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTAtt                                                                                 <  2:497336/150‑1 (MQ=255)

                                                                        gctttgaggccgcATCCGGCATGAACAAAGCCTACGTTGTCT‑ACAATATCAAAATGACGCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTgg                                                                               <  1:1040173/143‑1 (MQ=255)

                                                                        gCGTTCACGCCGCATCCTTCATTAACAAAGCCTACGTTGTCT‑ACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATTACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTgg                                                                               <  8:858115/151‑1 (MQ=255)

                                                                        gCGTTCACGCCGCATCCGGCATGAACAAAGCCTTCGTTGTAT‑ACAATATGAAAATGACGCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTgg                                                                               <  7:1276662/151‑1 (MQ=255)

                                                                         cGTTGACGCTGTATCCTGCATGAACAAAGCCTACTTTGTAT‑ACAATAATAAAATGACTCATTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGa                                                                              <  6:586604/151‑1 (MQ=17)

                                                                         cGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCT‑ACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTgg                                                                               >  3:968753/1‑150 (MQ=255)

                                                                          gTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCT‑ACAATATGAAAATGCCGCCTTTTACAGCGCCAGTTGAAACTGATGACAAACGCAACATTCCCTGATGAGCTCCGCTTATCAGGAATACACGGTCACTGCAATCTATAGGAt                                                                             >  5:1941319/1‑151 (MQ=21)

                                                                          cttCACGCCGCATCCGGCATGAACAAAGCCTACGATGTCT‑ACAATATGAAAATGACGCCTTTTACAGCCCCATTTGAAACTGAAGAAAAACGAAACATTAACTGATGACATACGCAAATCAGAACTACACGTACACAGCAATCTaaagca                                                                              >  8:376133/2‑145 (MQ=17)

                                                                           ttCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCT‑ACAATATGAAAATGACGCCTTTTACAGCGCCATTTTAAACTGATGAAAAAAGAAACATTGAATGATTAGATAAGATTATAAGGACTAAACGTTCACTTCAATATATTGGAtt                                                                            >  6:113254/1‑151 (MQ=17)

                                                                              aCGCCGCATCCGGCATGAACAAAGCCTACGTTGTCT‑ACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGa                                                                         <  3:193785/151‑1 (MQ=255)

                                                                              aCGCCGCATCCGGCATGAACAAAGCCTACGTTGTCT‑ACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGAAACATTGCCTGATGCGCTACGCTTATCAGGACTACACGTTCACTGCAATCTATTGGATTTg                                                                          >  2:1299378/1‑150 (MQ=255)

                                                                               cgactgaaccGGCATGAACAAAGCCTACGGTTTAC‑ACAATATCAAAATGCCCCCTTATACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGaa                                                                        <  5:307505/143‑1 (MQ=14)

                                                                                 ccgcATCCGGCATGAACAAAGCCTACGTTGTCT‑ACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGAAAAACGCAACATTGCATGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAgg                                                                      >  6:894877/1‑151 (MQ=255)

                                                                                      tCCGGCATGAACAAAGCCTACGTTGTCT‑ACAATATGAAAATGCCGCCTTTTACAGCGCCATTTTAAACTGATTCCAAACGCAACATTGCCTGAATCGCTACGAGTCTCAGTCCTACACGGTCAATGCCATATATTGGATTTGAAGGTtttt                                                                 >  2:1131117/1‑151 (MQ=18)

                                                                                       gcGGGATGAACAAAGCCTTCTTTTTCT‑ACAATATGAAAATGTCGCTTTTTTCAGCGCCATTTTCAACTGATGACTAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTGACTGCAATCTATTGGATTTGAAGGGtttt                                                                 <  3:436845/149‑1 (MQ=17)

                                                                                       ccGGCATGAACAAAGCCTACGTTGTCT‑ACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGTCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGtttt                                                                 >  7:989528/1‑150 (MQ=255)

                                                                                        cGGCATGAACAAAGCCTACGTTGTCT‑ACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGCGTTTTc                                                                >  7:1179191/1‑150 (MQ=255)

                                                                                          gaaTGAACAAATCCTTATTTTTAT‑ACAATATCAAAATGACTCATTATACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCAt                                                              <  5:411579/148‑1 (MQ=14)

                                                                                          gCATGAAGAATGTCTTCGTT‑TCTAACAATATCAAAATGTCTCTTTATAAAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATg                                                             <  2:731844/151‑1 (MQ=11)

                                                                                            aTGAACAAAGCCTACTTTTTTT‑AAATTATCATACTCACTCCTTTATCATCTCTATTTTAAACTGATTACAAATCCAAGATTGTCTTATGATCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGcc                                                           <  6:705938/151‑1 (MQ=2)

                                                                                            aTGAACAAAGCCTACGTTGTCT‑ACAATATGAAAATGACGCCTTTTACAGCGACATTTGAAACTGATGACAAACGCAACATTCCCTGATGCGATACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGcc                                                           >  3:892634/1‑151 (MQ=255)

                                                                                                  anaGCCTACGTTGTCT‑ACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGAAACATTGACTGATGAGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATa                                                      >  3:356320/1‑150 (MQ=255)

                                                                                                   aaGCCTTCTTTTTAT‑TCAATATAAAAATGTCTCATTATAAAGCGCCATTTTTAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAg                                                    <  6:1855888/151‑1 (MQ=11)

                                                                                                    aGCCTACGTTGTCT‑ACAATATGAAAATGACGCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAgg                                                   >  3:113610/1‑151 (MQ=255)

                                                                                                        tACGTTGTCT‑ACAATATGAAAATGACGCCTTTTAAAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGAGCTACGATTATCAGGCCTACACGTACACTGCAATCTATTGAATTTGAAGGGTTTTCATGACGGATAAGGCttt                                               >  8:1712212/1‑151 (MQ=17)

                                                                                                            ttGTCT‑ACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCAcg                                           >  4:645444/1‑151 (MQ=255)

                                                                                                                cT‑ACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCAcgccgc                                       >  4:407060/1‑151 (MQ=255)

                                                                                                                 t‑ACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATTCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGCTTTTCATGCCGGATAAGGCGTTCAcgccgc                                       >  2:1544250/1‑150 (MQ=255)

                                                                                                                 t‑ACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCAcgccgc                                       >  3:1522301/1‑150 (MQ=255)

                                                                                                                   caaaTATGAACCTGACGCCTTTTACACCCCCATTTGAAACTGCTGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCa                                      <  7:1224011/148‑1 (MQ=14)

                                                                                                                   aCAATATGTAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCAt                                     >  5:1342825/1‑151 (MQ=255)

                                                                                                                      atatGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCg                                  >  5:1730470/1‑151 (MQ=255)

                                                                                                                      agatcaaaaTGACCCCTTATACAGCGCCATTTCAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCg                                  <  3:1584239/146‑1 (MQ=255)

                                                                                                                        atGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGc                                >  6:1436459/1‑151 (MQ=255)

                                                                                                                         tGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGc                                >  7:263062/1‑150 (MQ=255)

                                                                                                                         tGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGc                                >  8:514906/1‑150 (MQ=255)

                                                                                                                              aTGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAAc                          >  2:402336/1‑151 (MQ=255)

                                                                                                                                gACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCAGCATCCGGCATGAACa                         >  6:1532089/1‑150 (MQ=255)

                                                                                                                                gACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATCCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACa                         >  1:992112/1‑150 (MQ=255)

                                                                                                                                   gCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGc                     >  3:70997/1‑151 (MQ=40)

                                                                                                                                       tttACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCt                   <  5:1223475/149‑1 (MQ=38)

                                                                                                                                       tttACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTa                  >  4:1709307/1‑150 (MQ=38)

                                                                                                                                        ttACAGCGCCATTTCAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTAc                 <  3:1838584/150‑1 (MQ=21)

                                                                                                                                         tACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACg                <  7:1689020/150‑1 (MQ=35)

                                                                                                                                          aCAGCTCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGtt              <  7:491900/151‑1 (MQ=25)

                                                                                                                                           cAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTg             >  5:1042005/1‑151 (MQ=34)

                                                                                                                                             gagcCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTc           <  8:1792192/149‑1 (MQ=14)

                                                                                                                                              cgcCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCt          >  2:1702146/1‑151 (MQ=33)

                                                                                                                                               gcCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCt          >  4:1421046/1‑150 (MQ=33)

                                                                                                                                                  aTTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGACTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACa       >  7:1015566/1‑150 (MQ=18)

                                                                                                                                                    ttGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAat     >  8:1540886/1‑150 (MQ=32)

                                                                                                                                                    ttGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAat     >  1:1193122/1‑150 (MQ=32)

                                                                                                                                                      gAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATg  >  7:815522/1‑151 (MQ=31)

ATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCT‑ACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATG  >  DH1/2326138‑2326437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`