Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH12,466,9620AT67.2% 584.8 / inf 426I145F (ATC→TTC) ttcAtRNA 2‑thiocytidine(32) synthetase TtcA
Reads supporting (aligned to +/- strand):  ref base A (62/77);  new base T (130/155);  total (193/233)
Fisher's exact test for biased strand distribution p-value = 9.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 426 total aligned reads displayed.

AGCTGGGCGTTGAGTACAAGATTGTTGAAGAGAATACTTACGGTATCGTGAAAGAGAAGATTCCAGAGGGCAAAACCACTTGCTCACTGTGTTCTCGCCTTCGTCGCGGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGATCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACGTTGTTCTTAAATATGTTCTACGGCGGTAAGATGAAAGGTATGCCTCCGAAACTGATGAGCGATGATGGCAAACATATCGTTATTCGTCCGCTGGCCTACTGCCGCGAGAAA  >  DH1/2466813‑2467111
                                                                                                                                                     |                                                                                                                                                     
aGCTGGGCGTTGAGTACAAGATTGTTTAAGAGAATACTTACGGTATCGTGAAATAGAAGATTCCAGAGGGCAAAACCACTTGCTCACTGTGTTCTCGCCTTCGTCGCGGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGAt                                                                                                                                                      <  6:1572776/151‑1 (MQ=255)
       cGTTGAGTACAAGATTGTTGAAGAGAATACTTACGGTATCGTGAAAGAGAAGATTCCAGAGGGCAAAACCACTTGCTCACTGTGTTCTCGCCTTCGTCGCGGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGATCGCGTTg                                                                                                                                               <  1:1434010/151‑1 (MQ=255)
          tGAGTACAAGATTGTTGAAGAGAATACTTACGGTATCGTGAAAGAGAAGATTCCAGAGGGCAAAACCACTTGCTCACTGTGTTCTCGCCTTCGTCGCGGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGTTCGCGTTggg                                                                                                                                             <  7:564736/150‑1 (MQ=255)
              tACAAGATTGTTGAAGAGAATACTTACGGTATCGTGAAAGAGAAGATTCCAGAGGGCAAAACCACTTGCTCACTGTGTTCTCGCCTTCGTCGCGGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGATCGCGTTGGGTCAcc                                                                                                                                        >  8:1344053/1‑151 (MQ=255)
                 aaGATTGTTGAAGAGAATACTTACGGTATCGTGAAAGTGAAGATTCCAGAGGGCAAAACCACTTGCTCACTGTGTTCTCGCCTTCGTCGCGGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGTTCGCGTTGGGTCACCATc                                                                                                                                     <  1:913276/151‑1 (MQ=255)
                 aaGATTGTTGAAGAGAATACTTACGGTATCGTGAAAGAGAAGATTCCAGAGGGCAAAACCACTTGCTCACTGTGTTCTCGCCTTCGTCGCGGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGTTCGCGTTGGGTCACCATc                                                                                                                                     <  6:1885635/151‑1 (MQ=255)
                   gATTGTTGAAGAGAATACTTACGGTATCGTGAATGAGAAGATTCCAGAGGGCAAAACCTCTTGCTCACTGTGTTCTCGCCTTCGTCGCGGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGATCGCGTTGGGTCACCATCGt                                                                                                                                   <  5:1936021/151‑1 (MQ=255)
                   gATTGTTGAAGAGAATACTTACGGTATCGTGAAAGAGAAGATTCCAGAGGGCAAAACCTCTTGCTCACTGTGTTCTCGCCTTCGTCGCGGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGATCGCGTTGGGTCACCATCGt                                                                                                                                   <  5:522976/151‑1 (MQ=255)
                    aTTGTTGAAGAGAATACTTACGGTATCGTGAAAGAGAAGATTCCAGAGGGCAAAACCACTTGCTCACTGTGTTCTCGCCTTCGTCGCGGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGTTCGCGTTGGGTCACCATCGTg                                                                                                                                  >  3:319258/1‑151 (MQ=255)
                       gttgAAGAGAATACTTACGGTATCGTGAAAGAGAAGATTCCAGAGGGCAAAACCACTTGCTCACTGTGTTCTCGCCTTCGTCGCGGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGTTCGCGTTGGGTCACCATCGTGCCg                                                                                                                               >  8:1609981/1‑151 (MQ=255)
                       gttgAAGAGAATACTTACGGTATCGTGAAAGAGAAGATTCCAGAGGGCAAAACCACTTGCTCACTGTGTTCTCGCCTTCGTCGCGGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGTTCGCGTTGGGTCACCATCGTGACg                                                                                                                               >  7:1957190/1‑151 (MQ=255)
                       gttgAAGAGAATACTTACGGTATCGTGAAAGAGAAGATTCCAGAGGGCAAAACCACTTGCTCACTGTGTTCTCGCCTTCGTCGCGGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGATCGCGTTGGGTCACCATCGTGACg                                                                                                                               <  8:28068/151‑1 (MQ=255)
                         tgAAGAGAATACTTACGGTATCGTGAAAGAGAAGATTCCAGAGGGCAAAACCACTTGCTCACTGTGTTCTCGCCTTCGTCGCGGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGTTCGCGTTGGGTCACCATCGTGACGat                                                                                                                             >  6:623715/1‑151 (MQ=255)
                          gAAGAGAATACTTACGGTATCGTGAAAGAGAAGATTCCAGAGGGCAAAACCACTTGCTCACTGTGTTCTCGCCTTCGTCGCGGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGATCGCGTTGGGTCACCATCGTGACGat                                                                                                                             >  4:116624/1‑150 (MQ=255)
                           aaGAGAATACTTACGGTATCGTGAAAGAGAAGATTCCAGAGGGCAAAACCACTTGCTCACTGTGTTCTCGCCTTCGTCGCGGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGATCGCGTTGGGTCACCATCGTGACGata                                                                                                                            <  2:280094/150‑1 (MQ=255)
                              agaATACTTACGGTATCGTGAAAGAGAAGATTCCAGAGGGCAAAACCACTTGCTCACTGTGTTCTCGCCTTCGTCGCGGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGATCGCGTTGGGTCACCATCGTGACGATATCCt                                                                                                                        <  8:1945945/151‑1 (MQ=255)
                                 aTACTTACGGTATCGTGAAAGAGAAGATTCCAGAGGGCAAAACCACTTGCTCACTGTGTTCTCGCCTTCGTCGCGGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGTTCGCGTTGGGTCACCATCGTGACGATATCCTGCa                                                                                                                     <  5:298422/151‑1 (MQ=255)
                                  tACTTACGGTATCGTGAAAGAGAAGATTCCAGAGGGCAAAACCACTTGCTCACTGTGTTCTCGCCTTCGTCGCGGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGTTCGCGTTGGGTCACCATCGTGACGATATCCTGCa                                                                                                                     >  3:1329578/1‑150 (MQ=255)
                                   aCTTACGGTATCGTGAAAGAGAAGATTCCAGAGGGCAAAACCACTTGCTCACTGTGTTCTCGCCTTCGTCGCGGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGATCGCGTTGGGTCACCATCGTGACGATATCCTGCaa                                                                                                                    >  6:1478489/1‑150 (MQ=255)
                                   aCTTACGGTATCGTGAAAGAGAAGATTCCAGAGGGCAAAACCACTTGCTCACTGTGTTCTCGCCTTCGTCGCGGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGATCGCGTTGGGTCACCATCGTGACGATATCATGCaa                                                                                                                    <  7:127773/150‑1 (MQ=255)
                                     ttACGGTATCGTGAAAGAGAAGATTCCAGAGGGCAAAACCACTTGCTCACTGTGTTCTCGCCTTCGTCGCGGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGTTCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACg                                                                                                                 >  7:679028/1‑151 (MQ=255)
                                      tACGGTATCGTGAAAGAGAAGATTCCAGAGGGCAAAACCACTTGCTCACTGTGTTCTCGCCTTCGTCGCGGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGTTCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACgt                                                                                                                >  3:497211/1‑151 (MQ=255)
                                        cGGTATCGTGAAAGAGAAGATTCCAGAGGGCAAAACCACTTGCTCACTGTGTTCTCGCCTTCGTCGCGGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGTTCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACgttg                                                                                                              <  1:960897/151‑1 (MQ=255)
                                        cGGTATCGTGAAAGAGAAGATTCCAGAGGGCAAAACCACTTGCTCACTGTGTTCTCGCCTTCGTCGCGGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGTTCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACgttg                                                                                                              <  5:100245/151‑1 (MQ=255)
                                        cGGTATCGTGAAACCGAAGATTGCAGACGGCAAAACCACTTGCTCACTGTGTTCTCGCCATCGGCGCGGTATGCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGTTCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACgttg                                                                                                              <  1:1074342/151‑1 (MQ=255)
                                           tATCGTGAAAGAGAAGATTCCAGAGGGCAAAACCACTTGCTCACTGTGTTCTCGCCTTCGTCGCGGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGTTCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACgttgtt                                                                                                            >  2:1678210/1‑150 (MQ=255)
                                            aTCGTGAAAGAGAAGATTCCAGAGGGCAAAACCACTTGCTCACTGTGTTCTCGCCTTCGTCGCGGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGTTCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACGTTGTTCt                                                                                                          >  2:864361/1‑151 (MQ=255)
                                            aTCGTGAAAGAGAAGATTCCAGAGGGCAAAACCACTTGCTCACTGTGTTCTCGCCTTCGTCGCGGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGTTCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACGTTGTTCt                                                                                                          >  8:119056/1‑151 (MQ=255)
                                              cGTGAAAGAGAAGATTCCAGAGGGCAAAACCACTTGCTCACTGTGTTCTCGCCTTCGTCGCGGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGTTCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACGTTGTTCTTa                                                                                                        <  6:742054/151‑1 (MQ=255)
                                               gTGAAAGAGAAGATTCCAGAGGGCAAAACCACTTGCTCACTGTGTTCTCGCCTTCGTCGCGGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGTTCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACGTTGTTCTTaa                                                                                                       <  7:787733/151‑1 (MQ=255)
                                               gTGAAAGAGAAGATTCCAGAGGGCAAAACCACTTGCTCACTGTGTTCTCGCCTTCGTCGCGGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGTTCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACGTTGTTCTTa                                                                                                        >  5:688760/1‑150 (MQ=255)
                                               gTGAAAGAGAAGATTCCAGAGGGCAAAACCACTTGCTCACTGTGTTCTCGCCTTCGTCGCGGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGATCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACGTTGTTCTTa                                                                                                        >  6:286777/1‑150 (MQ=255)
                                                tGAAAGAGAAGATTCCAGAGGGCAAAACCACTTGCTCACTGTGTTCTCGCCTTCGTCGCGGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGTTCGCGTTGGGTCACCATCGTGACGATCTCCTGCAAACGTTGGTCTTaa                                                                                                       >  8:1449781/1‑150 (MQ=255)
                                                tGAAAGAGAAGATTCCAGAGGGCAAAACCACTTGCTCACTGTGTTCTCGCCTTCGTCGCGGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGTTCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACGTTGTTCTTaa                                                                                                       >  8:838706/1‑150 (MQ=255)
                                                tGAAAGAGAAGATTCCAGAGGGCAAAACCACTTGCTCACTGTGTTCTCGCCTTCGTCGCGGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGATCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACGTTGTTCTTaa                                                                                                       >  6:974519/1‑150 (MQ=255)
                                                 gAAAGAGAAGATTCCAGAGGGCAAAACCACTTGCTCACTGTGTTCTCGCCTTCGTCGCGGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGTTCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACGTTGTTCTTAAat                                                                                                     >  2:1548270/1‑151 (MQ=255)
                                                  aaaGAGAAGATTCCAGAGGGCAAAACCACTTGCTCACTGTGTTCTCGCCTTCGTCGCGGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGTTCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACGTTGTTCTTAAata                                                                                                    <  1:102004/151‑1 (MQ=255)
                                                  aaaGAGAAGATTCCAGAGGGCAAAACCACTTGCTCACTGTGTTCTCGCCTTCGTCGCGGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGATCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACGTTGTTCTTAAat                                                                                                     >  7:1574859/1‑150 (MQ=255)
                                                   aaGAGAAGATTCCAGAGGGCAAAACCACTTGCTCACTGTGTTCTCGCCTTCGTCGCGGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGATCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACGTTGTTCTTAAatat                                                                                                   >  1:668099/1‑151 (MQ=255)
                                                    agagAAGATTCCAGAGGGCAAAACCACTTGCTCACTGTGTTCTCGCCTTCGTCGCGGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGATCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACGTTGTTCTTAAATATg                                                                                                  >  3:1797371/1‑151 (MQ=255)
                                                    agagAAGATTCCAGAGGGCAAAACCACTTGCTCACTGTGTTCTCGCCTTCGTCGCGGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGATCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACGTTGTTCTTAAATATg                                                                                                  >  3:615865/1‑151 (MQ=255)
                                                     gagaAGATTCCAGAGGGCAAAACCACTTGCTCACTGTGTTCTCGCCTTCGTCGCGGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGTTCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACGTTGTTCTTAAATATGt                                                                                                 >  4:134975/1‑151 (MQ=255)
                                                     gagaAGATTCCAGAGGGCAAAACCACTTGCTCACTGTGTTCTCGCCTTCGTCGCGGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGTTCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACGTTGTTCTTAAATATGt                                                                                                 >  1:1683151/1‑151 (MQ=255)
                                                     gagaAGATTCCAGAGGGCAAAACCACTTGCTCACTGTGTTCTCGCCTTCGTCGCGGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGATCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACGTTGTTCTTAAATATGt                                                                                                 <  3:1541698/151‑1 (MQ=255)
                                                      agaagaTTCCAGAGGGCAAAACCACTTGCTCACTGTGTTCTCGCCTTCGTCGCGGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGTTCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACGTTGTTCTTAAATATGtt                                                                                                <  3:1620233/151‑1 (MQ=255)
                                                          gaTTCCAGAGGGCAAAACCACTTGCTCACTGTGTTCTCGCCTTCGTCGCGGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGTTCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACGTTGTTCTTAAATATGTTCTAc                                                                                            >  3:1695449/1‑151 (MQ=255)
                                                          gaTTCCAGAGGGCAAAACCACTTGCTCACTGTGTTCTCGCCTTCGTCGCGGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGATCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACGTTGTTCTTAAATATGTTCTAc                                                                                            <  6:1712606/151‑1 (MQ=255)
                                                           aTTCCAGAGGGCAAAACCACTTGCTCACTGTGTTCTCGCCTTCGTCGCGGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGTTCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACGTTGTTCTTAAATATGTTCTAcg                                                                                           >  1:1571255/1‑151 (MQ=255)
                                                              ccAGAGGGCAAAACCACTTGCTCACTGTGTTCTCGCCTTCGTCGCGGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGTTCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACGTTGTTCTTAAATATGTTCTAcggcg                                                                                        >  1:300242/1‑151 (MQ=255)
                                                              ccAGAGGGCAAAACCACTTGCTCACTGTGTTCTCGCCTTCGTCGCGGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGTTCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACGTTGTTCTTAAATATGTTCTAcggc                                                                                         <  4:501522/150‑1 (MQ=255)
                                                              ccAGAGGGCAAAACCACTTGCTCACTGTGTTCTCGCCTTCGTCGCGGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGATCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACGTTGTTCTTAAATATGTTCTAcggcg                                                                                        <  1:1887480/151‑1 (MQ=255)
                                                               cAGAGGGCAAAACCACTTGCTCACTGTGTTCTCGCCTTCGTCGCGGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGTTCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACGTTGTTCTTAAATATGTTCTAcggcgg                                                                                       <  4:1038156/151‑1 (MQ=255)
                                                                 gagGGCAAAACCACTTGCTCAGTGTGTTCTCGCCTTCGTCGCGGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGATCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACGTTGTTCTTAAATATGTTCTTCGGCGGTa                                                                                     <  2:1148703/151‑1 (MQ=255)
                                                                  agGGCAAAACCACTTGCTCACTGTGTTCTCGCCTTCGTCGCGGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGATCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACGTTGTTCTTAAATATGTTCTACGGCGGTa                                                                                     >  1:247806/1‑150 (MQ=255)
                                                                    ggCAAAACCACTTGCTCACTGTGTTCTCGCCTTCGTCGCGGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGTTCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACGTTGTTCTTAAATATGTTCTACGGCGGTAAGa                                                                                  <  8:690826/151‑1 (MQ=255)
                                                                    ggCAAAACCACTTGCTCACTGTGTTCTCGCCTTCGTCGCGGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGTTCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACGTTGTTCTTAAATATGTTCTACGGCGGTAAGa                                                                                  <  4:1628666/151‑1 (MQ=255)
                                                                     gCAAAACCACTTGCTCACTGTGTTCTCGCCTTCGTCGCGGTATCCTTTATCGTACCGCAACGAAACTGGGGGCGACGAAGTTCGCGTTGGGGCACCATCGTGACGATATCCTGCAAACGTTGTTCTTAAATATGTTCTACGGCGGTAAGAt                                                                                 <  2:438381/151‑1 (MQ=255)
                                                                       aaaaCCACTTGCTCACTGTGTTCTCGCCTTCGTCGCGGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGTTCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACGTTGTTCTTAAATATGTTCTACGGCGGTAAGATg                                                                                >  4:752257/1‑150 (MQ=255)
                                                                       aaaaCCACTTGCTCACTGTGTTCTCGCCTTCGTCGCGGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGTTCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACGTTGTTCTTAAATATGTTCTACGGCGGTAAGATGa                                                                               <  1:466787/151‑1 (MQ=255)
                                                                         aaCCACTTGCTCACTGTGTTCTCGCCTTCGTCGCGGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGTTCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACGTTGTTCTTAAATATGTTCTACGGCGGTAAGATGaaa                                                                             <  5:1615394/151‑1 (MQ=255)
                                                                              cTTGCTCACTGTGTTCTCGCCTTCGTCGCGGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGTTCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACGTTGTTCTTAAATATGTTCTACGGCGGTAAGATGAAAGGTAt                                                                        >  2:379192/1‑151 (MQ=255)
                                                                              cTTGCTCACTGTGTTCTCGCCTTCGTCGCGGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGTTCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACGTTGTTCTTAAATATGTTCTACGGCGGTAAGATGAAAGGTAt                                                                        >  4:353218/1‑151 (MQ=255)
                                                                                   tCACTGTGTTCTCGCCTTCGTCGCGGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGTTCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACGTTGTTCTTAAATATGTTCTACGGCGGTAAGATGAAAGGTATGCCTc                                                                   >  8:1855006/1‑151 (MQ=255)
                                                                                    cACTGTGTTCTCGCCTTCGTCGCGGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGTTCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACGTTGTTCTTAAATATGTTCTACGGCGGTAAGATGAAAGGTATGCCTcc                                                                  >  7:1478989/1‑151 (MQ=255)
                                                                                     aCTGTGTTCTCGCCTTCGTCGCGGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGATCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACGTTGTTCTTAAATATGTTCTACGGCGGTAAGATGAAAGGTATGCCTCCg                                                                 >  2:1304983/1‑151 (MQ=255)
                                                                                     aCTGTGTTCTCGCCTTCGTCGCGGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGATCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACGTTGTTCTTAAATATGTTCTACGGCGGTAAGATGAAAGGTATGCCTCCg                                                                 >  2:749790/1‑151 (MQ=255)
                                                                                      cTGTGTTCTCGCCTTCGTCGCGGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGTTCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACGTTGTTCTTAAATATGTTCTACGGCGGTAAGATGAAAGGTATGCCTCCg                                                                 >  5:819814/1‑150 (MQ=255)
                                                                                      cTGTGTTCTCGCCTTCGTCGCGGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGTTCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACGTTGTTCTTAAATATGTTCTACGGCGGTAAGATGAAAGGTATGCCTCCg                                                                 >  1:136997/1‑150 (MQ=255)
                                                                                       tgtgTTCTCGCCTTCGTCGCGGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGATCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACGTTGTTCTTAAATATGTTCTACGGCGGTAAGATGAAAGGTATGCCTCCGa                                                                >  7:1876846/1‑150 (MQ=255)
                                                                                               cGCCTTCGTCGCGGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGATCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACGTTGTTCTTAAATATGTTCTACGGCGGTAAGATGAAAGGTATGCCTCCGAAACtgatg                                                        <  3:1431351/150‑1 (MQ=255)
                                                                                               cGCCTTCGTCGCGGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGATCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACGTTGTTCTTAAATATGTTCTACGGCGGTAAGATGAAAGGTATGCCTCCGAAACtgatg                                                        <  7:1362513/150‑1 (MQ=255)
                                                                                                 ccTTCGTCGCGGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGTTCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACGTTGTTCTTAAATATGTTCTACGGCGGTAAGATGAAAGGTATGCCTCCGAAACTGATGAGc                                                     <  3:441709/151‑1 (MQ=255)
                                                                                                      gtcgCGGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGTTCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACGTTGTTCTTAAATATGTTCTACGGCGGTAAGATGAAAGGTATGCCTCCGAAACTGATGAGCgatga                                                <  4:1781723/151‑1 (MQ=255)
                                                                                                        cgcgGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGTTCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACGTTGTTCTTAAATATGTTCTACGGCGGTAAGATGAAAGGTATGCCTCCGAAACTGATGAGCgatgat                                               <  5:1320223/150‑1 (MQ=255)
                                                                                                        cgcgGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGTTCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACGTTGTTCTTAAATATGTTCTACGGCGGTAAGATGAAAGGTATGCCTCCGAAACTGATGAGCGatgatg                                              <  6:1043691/151‑1 (MQ=255)
                                                                                                         gcgGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGTTCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACGTTGTTCTTAAATATGTTCTACGGCGGTAAGATGAAAGGTATGCCTCCGAAACTGATGAGCGATGATgg                                             >  6:1205526/1‑151 (MQ=255)
                                                                                                         gcgGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGATCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACGTTGTTCTTAAATATGTTCTACGGCGGTAAGATGAAAGGTATGCCTCCGAAACTGATGAGCGATGATgg                                             >  2:660981/1‑151 (MQ=255)
                                                                                                          cgGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGATCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACGTTGTTCTTAAATATGTTCTACGGCGGTAAGATGAAAGGTATGCCTCCGAAACTGATGAGCGATGATGGc                                            <  5:1426557/151‑1 (MQ=255)
                                                                                                           ggTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGTTCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACGTTGTTCTTAAATATGTTCTACGGCGGTAAGATGAAAGGTATGCCTCCGAAACTGATGAGCGATGATGGc                                            >  7:1731298/1‑150 (MQ=255)
                                                                                                            gTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGATCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACGTTGTTCTTAAATATGTTCTACGGCGGTAAGATGAAAGGTATGCCTCCGAAACTGATGAGCGATGATGGCa                                           >  3:116366/1‑150 (MQ=255)
                                                                                                              aTCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGATCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACGTTGTTCTTAAATATGTTCTACGGCGGTAAGATGAAAGGTATGCCTCCGAAACTGATGAGCGATGATGGCAAAc                                        >  3:791100/1‑151 (MQ=255)
                                                                                                               tCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGTTCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACGTTGTTCTTAAATATGTTCTACGGCGGTAAGATGAAAGGTATGCCTCCGAAACTGATGAGCGATGATGGCAAAc                                        >  3:1878520/1‑150 (MQ=255)
                                                                                                                  tttATCGTACCGCAACGGAACTGGGGGCGACGAAGTTCGCGTTGGGTCACCATCGTGCCGATATCCTGCCAACGTTGTTCGTAAATATGTTCTACGGCGGTAAGATGAAAGGTATGCCTCCGAAACTGATGAGCGATGATGGCAAACatat                                    >  4:425287/1‑151 (MQ=255)
                                                                                                                   ttATCGTACCGCAACGGAACTGGGGGCGACGAAGTTCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACGTTGTTCTTAAATATGTTCTACGGCGGTAAGATGAAAGGTATGCCTCCGAAACTGATGAGCGATGATGGCAAACatat                                    <  1:840542/150‑1 (MQ=255)
                                                                                                                   ttATCGTACCGCAACGGAACTGGGGGCGACGAAGATCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACGTTGTTCTTAAATATGTTCTACGGCGGTAAGATGAAAGGTATGCCTCCGAAACTGATGAGCGATGATGGCAAACATATc                                   >  2:1824796/1‑151 (MQ=255)
                                                                                                                    tATCGTACCGCAACGGAACTGGGGGCGACGAAGATCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACGTTGTTCTTAAATATGTTCTACGGCGGTAAGATGAAAGGTATGCCTCCGAAACTGATGAGCGATGATGGCAAACATATc                                   <  8:1033120/150‑1 (MQ=255)
                                                                                                                       cGTACCGCAACGGAACTGGGGGCGACGAAGTTCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACGTTGTTCTTAAATATGTTCTACGGCGGTAAGATGAAAGGTATGCCTCCGAAACTGATGAGCGATGATGGCAAACATATCGtt                                <  2:525934/150‑1 (MQ=255)
                                                                                                                       cGTACCGCAACGGAACTGGGGGCGACGAAGTTCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACGTTGTTCTTAAATATGTTCTACGGCGGTAAGATGAAAGGTATGCCTCCGAAACTGATGAGCGATGATGGCAAACATATCGtt                                >  4:640501/1‑150 (MQ=255)
                                                                                                                        gTACCGCAACGGAACTTGGGGCGACGAAGATCGCGTGGGGTCACCATCGTTACGATATCCTGCAAACGTGGTGCTTAAATATGTTCTACTGCGGTAAGATGAAATGTATGCCTCCGAAACTGATGAGCGATTATGGCAAACATATCGTTAt                              <  1:1309928/151‑1 (MQ=255)
                                                                                                                         tACCGCAACGGAACTGGGGGCGACGAAGTTCGCGTTCGGTCACCATCGTGACGATATCATGCAAACGTTGTTCTTAAATATGTTCTACGGCGGTAAGAGGAAACGTAAGCCTCCGAAACTGATGAGCCATGATGGCAAACATATCCTTAtt                             >  7:455851/1‑151 (MQ=255)
                                                                                                                              cAACGGAACTTGGGTCGAGTAAGTTCGCGTTGGGTCACCATCGTGTCGATATCCTGCAATCGTTGTTCTTAAATATGTTATACGGCGGTAAGATGAAAGGTATGCGTCCGATTCTGATGAGCGTTGATGGAAAACATATCGTTATTCGTc                         <  4:264767/150‑1 (MQ=255)
                                                                                                                                 cGGAACTGGGGGCGACGAAGATCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACGTTGTTCTTAAATATGTTCTACGGCGGTAAGATGAAAGGTATGCCTCCGAAACTGATGAGCGATGATGGCAAACATATCGTTATTCGTCCGCt                     >  8:755247/1‑151 (MQ=255)
                                                                                                                                   gAACTGGGGGCGACGAAGTTCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACGTTGTTCTTAAATATGTTCTACGGCGGTAAGATGAAAGGTATGCCTCCGAAACTGATGAGCGATGATGGCAAACATATCGTTATTCGTCCGCTgg                   >  6:710960/1‑151 (MQ=255)
                                                                                                                                        gggggCGACGAAGATCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACGTTGTTCTTAAATATGTTCTACGGCGGTAAGATGAAAGGTATGCCTCCGAAACTGATGAGCGATGATGGCAAACATATCGTTATTCGTCCGCTGGCCTa               <  6:1315612/150‑1 (MQ=255)
                                                                                                                                         ggggCGACGAAGTTCGCGTTGGGTCACCATCGTGACGACATCCTGCAAACGTTGTTCTTAAATATGTTCTACGGCGGTAAGATGAAAGGTATGCCTCCGAAACTGATGAGCGATGATGGCAAACATATCGTTATTCGTCCGCTGGCCTACt             <  3:951821/151‑1 (MQ=255)
                                                                                                                                            gCGACGAAGTTCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACGTTGTTCTTAAATATGTTCTACGGCGGTAAGATGAAAGGTATGCCTCCGAAACTGATGAGCGATGATGGCAAACATATCGTTATTCGTCCGCTGGCCTACTGcc          <  6:460554/151‑1 (MQ=255)
                                                                                                                                              gacgaAGTTCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACGTTGTTCTTAAATATGTTCTACGGCGGTAAGATGAAAGGTATGCCTCCGAAACTGATGAGCGATGATGGCAAACATATCGTTATTCGTCCGCTGGCCTACTGCcgc        >  7:1667559/1‑151 (MQ=255)
                                                                                                                                               acgaAGTTCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACGTTGTTCTTAAATATGTTCTACGGCGGTAAGATGAAAGGTATGCCTCCGAACCTGATGAGCGATGATGGCAAACATATCGTTATTCGTCCGCTGGCCTACTGCcgcg       <  7:1112908/151‑1 (MQ=255)
                                                                                                                                               acgaAGTTCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACGTTGTTCTTAAATATGTTCTACGGCGGTAAGATGAAAGGTATGCCTCCGAAACTGATGAGCGATGATGGCAAACATATCGTTATTCGTCCGCTGGCCTACTGCcgcg       <  8:472962/151‑1 (MQ=255)
                                                                                                                                                  aaGATCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACGTTGTTCTTAAATATGTTCTACGGCGGTAAGATGAAAGGTATGCCTCCGAAACTGATGAGCGATGATGGCAAACATATCGTTATTCGTCCGCTGGCCTACTGCCGCgaga    <  5:1586533/151‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGCTGGGCGTTGAGTACAAGATTGTTGAAGAGAATACTTACGGTATCGTGAAAGAGAAGATTCCAGAGGGCAAAACCACTTGCTCACTGTGTTCTCGCCTTCGTCGCGGTATCCTTTATCGTACCGCAACGGAACTGGGGGCGACGAAGATCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACGTTGTTCTTAAATATGTTCTACGGCGGTAAGATGAAAGGTATGCCTCCGAAACTGATGAGCGATGATGGCAAACATATCGTTATTCGTCCGCTGGCCTACTGCCGCGAGAAA  >  DH1/2466813‑2467111

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑