Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH12,965,8990CG25.5% 359.5 / 69.2 204intergenic (+132/‑363)aqpZ/ybjEaquaporin/transporter
Reads supporting (aligned to +/- strand):  ref base C (23/129);  new base G (29/23);  total (52/152)
Fisher's exact test for biased strand distribution p-value = 4.70e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 243 total aligned reads displayed.

GCTGGAAAAGCGTGATTAATTAACGCAGAAGGCCTGGCGTAATGTCAGGCCTTTTTTTAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACATATTGCCACTGGCGCAAGGAGCGC‑GC‑AGGGGGCGGCCA‑ATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTG  >  DH1/2965749‑2966049
                                                                                                                                                        |                                                                                                                                                       
gctgGAAAAGCGTGATTAATTAACGCAGAAGGCCTGGCGTAATGTCAGGCCTTTTTTTAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACATATTGCCACTGGCGCAAGGAGCGC‑GC‑AGGGggc                                                                                                                                                         <  3:1942400/151‑1 (MQ=255)
  tgGAAAAGCGTGATTAATTAACGCAGAAGGCCTGGCGTAATGTCAGGCCTTTTTTTAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACATATTGCCACTGGCGCAAGGAGCGCGGC‑CGGGggcg                                                                                                                                                        >  2:1719751/1‑151 (MQ=255)
  tgGAAAAGCGTGATTAATTAACGCAGAAGGCCTGGCGTAATGTCAGGCCTTTTTTTAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACATATTGCCACTGGCGCAAGGAGCGC‑GG‑Ggcgggcgg                                                                                                                                                       >  8:706669/1‑151 (MQ=255)
    gAAAAGCGTGATTAATTAACGCAGAAGGCCTGGCGTAATGTCAGGCCTTTTTTTAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACATATTGCCACTGGCGCAAGGAGCGC‑GG‑Cgggggggggc                                                                                                                                                     >  4:1861578/1‑149 (MQ=255)
      aaaGCGTGATTAATTAACGCAGAAGGCCTGGCGTAATGTCAGGCCTTTTTTTAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACATATTGCCACTGGCGCAAGGAGCGC‑Gg‑ggggggggggggg                                                                                                                                                   >  5:1735586/1‑147 (MQ=255)
      aaaGCGTGATTAATTAACGCAGAAGGCCTGGCGTAATGTCAGGCCTTTTTTTAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACATATTGCCACTGGCGCAAGGAGCGC‑Gg‑gggggggggggcg                                                                                                                                                   >  2:608163/1‑147 (MQ=255)
       aaGCGTGATTAATTAACGCAGAAGGCCTGGCGTAATGTCAGGCCTTTTTTTAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACATATTGCCACTGGCGCAAGGAGCGCGGC‑GGGGGGGGGccc                                                                                                                                                    >  6:655715/1‑149 (MQ=255)
       aaGCGTGATTAATTAACGCAGAAGGCCTGGCGTAATGTCAGGCCTTTTTTTAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACATATTGCCACTGGCGCAAGGAGCGC‑Gg‑gggggggggggccc                                                                                                                                                  >  4:962151/1‑146 (MQ=255)
       aaGCGTGATTAATTAACGCAGAAGGCCTGGCGTAATGTCAGGCCTTTTTTTAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACATATTGCCACTGGCGCAAGGAGCGC‑GC‑CCGGGGGGGcccac                                                                                                                                                  >  3:145823/1‑148 (MQ=255)
        aGCGTGATTAATTAACGCAGAAGGCCTGGCGTAATGTCAGGCCTTTTTTTAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACATATTGCCACTGGCGCAAGGAGCGC‑GC‑AGGGGGCGGCCA‑ATc                                                                                                                                                <  6:1447924/151‑1 (MQ=255)
        aGCGTGATTAATTAACGCAGAAGGCCTGGCGTAATGTCAGGCCTTTTTTTAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACATATTGCCACTGGCGCAAGGAGCGC‑GC‑AGGGGGCGGCCA‑ATc                                                                                                                                                <  2:1542679/151‑1 (MQ=255)
         gCGTGATTAATTAACGCAGAAGGCCTGGCGTAATGTCAGGCCTTTTTTTAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACATATTGCCACTGGCGCAAGGAGCGC‑Gg‑ggggggggggcccgac                                                                                                                                                >  1:1705172/1‑144 (MQ=255)
         gCGTGATTAATTAACGCAGAAGGCCTGGCGTAATGTCAGGCCTTTTTTTAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACATATTGCCACTGGCGCAAGGAGCGC‑GC‑AGGGGGCGGCCA‑ATcg                                                                                                                                               <  7:1634186/151‑1 (MQ=255)
          cGTGATTAATTAACGCAGAAGGCCTGGCGTAATGTCAGGCCTTTTTTTAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACATATTGCCACTGGCGCAAGGAGCGC‑GG‑AAGGGGCGGCCa‑aaagc                                                                                                                                              >  6:1290587/1‑147 (MQ=255)
          cGTGATTAATTAACGCAGAAGGCCTGGCGTAATGTCAGGCCTTTTTTTAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACATATTGCCACTGGCGCAAGGAGCGC‑GC‑AGGGGGCGGCCA‑ATcgc                                                                                                                                              <  7:1295421/151‑1 (MQ=255)
           gTGATTAATTAACGCAGAAGGCCTGGCGTAATGTCAGGCCTTTTTTTAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACATATTGCCACTGGCGCAAGGAGCGC‑Gg‑ggggggggggggcccccc                                                                                                                                              >  7:607566/1‑142 (MQ=255)
            tGATTAATTAACGCAGAAGGCCTGGCGTAATGTCAGGCCTTTTTTTAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACATATTGCCACTGGCGCAAGGAGCGCGGC‑GGGGGGGGGcccaagccc                                                                                                                                              >  5:1928330/1‑144 (MQ=255)
            tGATTAATTAACGCAGAAGGCCTGGCGTAATGTCAGGCCTTTTTTTAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACATATTGCCACTGGCGCAAGGAGCGC‑Gg‑ggggggggggggggggggg                                                                                                                                             >  7:1687564/1‑141 (MQ=255)
              attaattaACGCAGAAGGCCTGGCGTAATGTCAGGCCTTTTTTTAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACATATTGCCACTGGCGCAAGGAGCGC‑GC‑AGGGGGCGGCCA‑ATcgccgcc                                                                                                                                          <  2:1359260/151‑1 (MQ=255)
               ttaattaaCGCAGAAGGCCTGGCGTAATGTCAGGCCTTTTTTTAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACATATTGCCACTGGCGCAAGGAGCGC‑Gg‑gggggggggggccgcccccccc                                                                                                                                          >  1:597780/1‑138 (MQ=255)
               ttaattaaCGCAGAAGGCCTGGCGTAATGTCAGGCCTTTTTTTAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACATATTGCCACTGGCGCAAGGAGCGC‑Gg‑ggggggggggcgctccaccccc                                                                                                                                          >  7:232144/1‑138 (MQ=255)
               ttaattaaCGCAGAAGGCCTGGCGTAATGTCAGGCCTTTTTTTAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACATATTGCCACTGGCGCAAGGAGCGC‑Gg‑ggggggggggcccccccccccc                                                                                                                                          >  3:1831104/1‑138 (MQ=255)
                taattaaCGCAGAAGGCCTGGCGTAATGTCAGGCCTTTTTTTAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACATATTGCCACTGGCGCAAGGAGCGC‑Gg‑ggggggggggcgcccgcccccgc                                                                                                                                         >  7:1039945/1‑137 (MQ=255)
                 aattaaCGCAGAAGGCCTGGCGTAATGTCAGGCCTTTTTTTAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACATATTGCCACTGGCGCAAGGAGCGCGGC‑CGGGGGGGGcccaaccccccccc                                                                                                                                         >  3:47337/1‑139 (MQ=255)
                 aattaaCGCAGAAGGCCTGGCGTAATGTCAGGCCTTTTTTTAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACATATTGCCACTGGCGCAAGGAGCGC‑Gg‑ggggggggggcccgccgccccccc                                                                                                                                        >  3:650635/1‑136 (MQ=255)
                    taaCGCAGAAGGCCTGGCGTAATGTCAGGCCTTTTTTTAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACATATTGCCACTGGCGCAAGGAGCGC‑GC‑AGGGGGCGGCCA‑ATCGCCGCCGcccc                                                                                                                                     <  6:1764792/150‑1 (MQ=255)
                     aaCGCAGAAGGCCTGGCGTAATGTCAGGCCTTTTTTTAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACATATTGCCACTGGCGCAAGGAGCGC‑GC‑GCGGGGGGGCCC‑AGCCCCCCCGcccccc                                                                                                                                   >  4:1021553/1‑150 (MQ=255)
                     aaCGCAGAAGGCCTGGCGTAATGTCAGGCCTTTTTTTAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACATATTGCCACTGGCGCAAGGAGCGC‑GC‑AGGGGGCGGCCA‑ATCGCCGCCGCCCCCt                                                                                                                                   <  4:1692347/151‑1 (MQ=255)
                        ccAGAAGGCCTGGCGTAATGTCAGGCCTTTTTTTAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACATATTGCCACTGGCGCAAGGAGCGC‑GC‑AGGGGGCGGCCA‑ATCGCCGCCGCCCCCTGCa                                                                                                                                <  3:1778260/150‑1 (MQ=255)
                          aGAAGGCCTGGCGTAATGTCAGGCCTTTTTTTAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACATATTGCCACTGGCGCAAGGAGCGCGGC‑GGGGGGGGGCCATAGCGccccccccccgcccc                                                                                                                                >  2:203075/1‑146 (MQ=255)
                          aGAAGGCCTGGCGTAATGTCAGGCCTTTTTTTAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACATATTGCCACTGGCGCAAGGAGCGCGGC‑CGGGGGGGCCCC‑AACCCCGccccccccccacc                                                                                                                               >  6:649201/1‑146 (MQ=255)
                             aGGCCTGGCGTAATGTCAGGCCTTTTTTTAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATCAGCTAAGCAACATATTGCCACTGGCGCAAGGAGCGC‑GC‑AGGGGGCGGCCA‑ATCGCCGCCGCCCCCTGCAccccc                                                                                                                           <  8:610260/151‑1 (MQ=255)
                               gCCTGGCGTAATGTCAGGCCTTTTTTTAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACATATTGCCACTGGCGCAAGGAGCGC‑GC‑AGGCGGCGGCCA‑ATCGCCGCCGCCCCCTGCACCCCCgg                                                                                                                         <  6:1671966/151‑1 (MQ=255)
                                 cTGGCGTAATGTCAGGCCTTTTTTTAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACATATTGCCACTGGCGCAAGGAGCGC‑GC‑CCGGGGGGGCCACAACCCCCCCGCCCCCCCCAcccccccc                                                                                                                        >  6:845059/1‑148 (MQ=255)
                                   ggCGTAATGTCAGGCCTTTTTTTAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACATATTGCCACTGGCGCAAGGAGCGCGGG‑CGGGGGGGGCCA‑AAcccccccccccccccccccccccgccc                                                                                                                      >  2:1232706/1‑146 (MQ=255)
                                    gcttattgtacggCTTTTTTTTACGATTTTTTTATCTGTTGTTTCAGAATGTTGTTGCGCTCCTTTCATTCCCAACGCAAAATATTGCCACTGGCGCAGGGCGGGC‑GC‑CGGGGGGGGCCA‑ACCCCCCCCGCCCCCTGCACCCCCGGGctct                                                                                                                    <  5:38133/140‑1 (MQ=12)
                                     cGTATTGTCAGCCCTTTTTTTAAGATGTTGTTATATGTTCATTCATCATCTTGTTGCGCTCCTTTCATCACCTAAGCAACATATTGCCCCTGGCGCAAGGAGCGC‑GC‑AGGGGGCGGCCA‑ATCCCCGCCGCCCCCTGCACCCCCGGGCTCTg                                                                                                                   <  6:1892803/151‑1 (MQ=25)
                                     cGTAATGTCAGGCCTTTTTTTAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACATATTGCCACTGGCGCAAGGAGCGC‑GG‑GGGGGGGCGGCA‑AAAGACGCCGCCCCCCCGAcccccccgcgc                                                                                                                     >  6:1950490/1‑143 (MQ=255)
                                        attGTCTGGCCTTTTTTTAAGATGTTTTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACATATTGCCACTGGCGCAAGGAGCGC‑GC‑TGGGGGCGGCCA‑ATCGCGGCCGCCCCCTGCACCCCCGGGCTCTGGc                                                                                                                 <  2:670193/148‑1 (MQ=255)
                                         aTGTCAGGCCTTTTTTTAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACATATTGCCACTGGCGCAAGGAGCGCGGCGGGGGGGGGGCCA‑ACCCCCCCCCCCCCCCCCCCCCCCgcggcggggg                                                                                                                 >  7:1263787/1‑142 (MQ=255)
                                           gTCAGGCCTTTTTTTAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACATATTGCCACTGGCGCAAGGAGCGC‑GC‑AGGGGGCGGCCA‑ATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAAc                                                                                                             <  4:530369/151‑1 (MQ=255)
                                            tCAGGCCTTTTTTTAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACATATTGCCACTGGCGCAAGGAGCGC‑GC‑CCGGGGCGGCCC‑AACGCCGCCCCCCCCAGCccccccccgggcaggggcac                                                                                                             >  2:44994/1‑136 (MQ=37)
                                              aGGCCTTTTTTTAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACATATTGCCACTGGCGCAAGGAGCGCGGG‑CGGGGGCGCCCA‑AACGCAccccccccccccccccccccccccctccccaaa                                                                                                            >  4:195110/1‑135 (MQ=255)
                                                 ccTTTTTTTAATATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAACCAACATATTGCCACTGGCGCAAGGCGCGC‑GC‑AGGGGGCGCCCA‑ACCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATc                                                                                                       <  5:1203498/151‑1 (MQ=255)
                                                 ccTTTTTTTAAGTTGTTTTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGCGCTAACCAAAATATTGCCCCTGGCGCAAGGAGCGC‑GC‑TGGGGGCGGCCA‑ATCGCGGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATc                                                                                                       <  8:896818/151‑1 (MQ=21)
                                                  cTTTTTTTAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACATATTGCCACTGGCGCAAGGAGCGC‑GG‑CGGGGGGGGCCC‑AAACCCGCCCCCCCCCCCCCCCCCCGGCGCAGGCGCCCaaaaaa                                                                                                       >  7:1643945/1‑148 (MQ=255)
                                                    ttttttAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACATATTGCCACTGGCGCAAGGAGCGC‑GC‑AGGGGGCGGCCA‑ATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATcgc                                                                                                     <  8:1955886/150‑1 (MQ=255)
                                                    ttttttAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACATATTGCCACTGGCGCAAGGAGCGC‑GC‑AGGGGGCGGCCA‑ATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATcgc                                                                                                     <  1:375887/150‑1 (MQ=255)
                                                      tttaacgATGTTTTTATTTGTTCATTCATCATCTTGTGGCGCCCCTTGCTGGCGCTAACCAACATATTCCCCCGGGCGCACGGCGCGC‑GC‑CGGGGGCGGCCA‑ACCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATcgccg                                                                                                   <  8:1614914/144‑1 (MQ=17)
                                                         tAAGTTTTTGTTTTTTGTTGATTTTTCATTTTGTTGCGGTCTTTTCATGAGCTATGCAACATATTGCCACTGGCGCATGGAGCGG‑GC‑AGGGGGCGGCCT‑ATGGCGGCGGCCCCGTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTg                                                                                                <  6:301508/150‑1 (MQ=17)
                                                             aTGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACATATTGCCACTGGCGCAAGGAGCGC‑GG‑GGGGGGGGGGCC‑AGAGGCGGCCCCCCCGGCCCCCCCCGGCGCGGGGGGACCAAAAcgcccgcgggcgg                                                                                           >  6:1959941/1‑142 (MQ=255)
                                                               gttgttTTTTGTTGATCCATCATCTTGTTGCGGTCCTTTCTTGAGCTAATAAAAATTTTGCCAGTGGCGCAAGCCGCGC‑GC‑TGGGGGGGGCAA‑ACCCCCGCCGCCCCCGGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGt                                                                                          <  6:544377/150‑1 (MQ=17)
                                                                ttgttATATGTTTATTCATCATGTTGTTGCGCTCTTTTCATGAGCTAAGCAACATATTGCCACTGGCGCAAGGAGCGC‑GC‑AGGGGGCGGCCA‑ATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTg                                                                                         <  2:511069/150‑1 (MQ=255)
                                                                 tgttATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACATATTGCCACTGGCGCAAGGAGCGC‑GC‑AGGGGGCGGCCA‑ATCGCCGCCCCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGc                                                                                        <  1:230969/150‑1 (MQ=255)
                                                                            taTTCATCAGCTTGTTGCGCTCCTTCCATGAGCTAACCAACATATTGCCACTGGCGCACGGAGCGC‑GC‑TGGGGGCGGCCA‑AGCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTAt                                                                             <  7:34284/149‑1 (MQ=18)
                                                                               tcttcatgTTGTTGCGCTCGTTTCATGTGCTAAGCAACATATTGCCACTGGCGCAAGCCGCGC‑GC‑AGGGGGCGGCGA‑ATCGCCGCGGCCCCGTGCACCCCCGGGCTCTGGGGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATccc                                                                          <  6:1173931/147‑1 (MQ=17)
                                                                               tcatcaTCTTGTTGCGCTCCTTTCATGAGCTAAGCAACATATTGCCACTGGCGCAAGGCGCGC‑GC‑AGGGGGCGGCCA‑ATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATccc                                                                          <  8:1515389/150‑1 (MQ=255)
                                                                               tcatcaTCTTGTTGCGCTCCTTTCATGAGCTAAGCAACATATTGCCACTGGCGCAAGGAGCGC‑GC‑TGGGGGCGGCCA‑ATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATccc                                                                          <  4:465942/150‑1 (MQ=255)
                                                                                cctcatcTTCTTGCGCTCCTTCCATGAGCTAAGCAACATATTGCCACTGCCGCACGGAGCGC‑GC‑AGGGGGCGGCCA‑ATCGCCGCCGCCCCCGGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCt                                                                         <  4:1200693/148‑1 (MQ=18)
                                                                                catcatCTTGTTGCGCGCCTTTCATCAGCTACGCAACATATTGCCACTGGCCCACGGCGCGC‑GC‑AGGGGGCGGCCA‑AGCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCtt                                                                        <  3:886016/151‑1 (MQ=18)
                                                                                   catcTTGTTGCGCTCCTTTCATGAGCTAAGCAACATATTGCCACTGGCGCAAGGAGCGC‑GC‑AGGGGGCGGCCA‑ATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTAc                                                                      <  3:1089290/150‑1 (MQ=255)
                                                                                   catcTTGTTGCGCTCCTTTCATGAGCTAAGCAACATATTGCCACTGGCGCAAGGAGCGC‑GC‑AGGGGGCGGCCA‑ATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTAc                                                                      <  1:1004758/150‑1 (MQ=255)
                                                                                   cagcTTGTTGCGCTCCTTTCATGAGCTAAGCAACATATTGCCACTGGCGCAAGGTGGGC‑GC‑AGGGGGCGGCCA‑ATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTAc                                                                      <  6:915585/147‑1 (MQ=255)
                                                                                     tgttgttgCGCGCCTTTAATGCGTTAAGAAACATATTGCCCCTGGCGCATGGCGCGG‑GC‑TGGGGGGGGCCA‑ATCGCGGCCGCCCCCGGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCGCTTACg                                                                     <  6:1558686/147‑1 (MQ=17)
                                                                                        tgtggCGCTCCTTTCATGAGCTAAGCAACATATTGCCACTGGCGCAAGGAGCGC‑GC‑AGGGGGCGGCCA‑ATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTa                                                                 <  1:407941/150‑1 (MQ=255)
                                                                                            gcgcgcCTTTCATGAGCTAAGCAACATATTGCCACTGGCGCAAGGAGCGC‑GC‑AGGGGGCGGCCA‑ATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCgg                                                             <  6:1021099/150‑1 (MQ=255)
                                                                                             ggcTCCTTTCATTATCAAACAAACATTTTTCCAGGGGGCCACGGCGCGG‑GC‑AGGGGGGGGCCA‑ATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGt                                                           <  8:1233015/150‑1 (MQ=11)
                                                                                             cgcTCCTTTCATCCGATAAGCAACATATTGCCACTGGCGCACGGAGCGC‑GC‑CGGGGGCGGCCA‑CTCGCGGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGt                                                           <  3:1315711/151‑1 (MQ=17)
                                                                                              gcTCCTTTCATGAGCTAAGCAACATATTGCCACTGGCGCAAGGAGCGC‑GC‑AGGGGGCGGCCA‑ATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTc                                                          <  4:809660/151‑1 (MQ=255)
                                                                                                gccTTTCATGAGCTAAGCAAAATATTGCCACTGGCGCATGGAGCGC‑GC‑TGGGGGCGGCAA‑ATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCgg                                                        <  2:1492824/150‑1 (MQ=255)
                                                                                                 ccTTTCATGAGCTAAGCAACATATTGCCACTGGCGCAAGGAGCGC‑GC‑AGGGGGCGGCCA‑ATCCCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCggg                                                       <  5:1140652/151‑1 (MQ=255)
                                                                                                     tcatcaGCTAAGCAACATATTGCCACTGGCGCAAGGAGCGC‑GC‑AGGGGGCGGCCA‑ATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCGGgcgc                                                    <  6:1500016/150‑1 (MQ=255)
                                                                                                      cATGAGCTAAGCAACATATTGCCACTGGCGCAAGGAGCGC‑GC‑AGGGGGCGGCCA‑ATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCGGGcgcg                                                   <  1:108933/150‑1 (MQ=255)
                                                                                                      cATGAGCTAAGCAACATATTGCCACTGGCGCAAGGAGCGC‑GC‑AGGGGGCGGCCA‑ATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCGGGCGCGa                                                  <  5:1900512/151‑1 (MQ=255)
                                                                                                      cATGAGCTAAGCAACATATTGCCACTGGCGCAAGGAGCGC‑GC‑AGGGGGCGGCCA‑ATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCGGGCGCGa                                                  <  2:1140215/151‑1 (MQ=255)
                                                                                                       aTGAGCTAAGCAACATATTGCCACTGGCGCAAGGAGCGC‑GC‑AGGGGGCGGCCA‑TGCGCCGCCGCCCCGTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCGGGCGCGAg                                                 <  1:863145/151‑1 (MQ=255)
                                                                                                         gcgcaaaGAAACATTTTCCCCCTGGCGCACGGCGCGC‑GC‑TGGGGGCGGCCA‑AGGGCCGCCGCCCCCGGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCGGGCGCGAgg                                                <  1:920422/145‑1 (MQ=11)
                                                                                                         gAGCTAAGCAACATATTGCCACGGGCGCAAGGAGCGC‑GC‑AGGGGGCGGCCA‑AGCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGt                                               <  5:147479/151‑1 (MQ=255)
                                                                                                         gAGCTAAGAAAAATATTGCCACTGGCGCACGGAGCGC‑GC‑AGGGGGGGGCCA‑ATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGt                                               <  2:1882238/151‑1 (MQ=255)
                                                                                                               aGCAACATATTGCCACTGGCGCAAGGCGGGC‑GC‑AGGGGGCGGCCA‑ATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATc                                         <  2:832255/151‑1 (MQ=255)
                                                                                                                    aatatTGCCAGGGGCGCATGGACCGC‑GC‑TGGGGGGGGCCA‑ATCGCCGCCGCCCCGGGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGt                                    <  8:349421/150‑1 (MQ=11)
                                                                                                                    aatatTCCCACTGGCGCAAGGCGCGC‑GC‑CGGGGGCGGCCA‑ATCGCCGCCGCCCCCGGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGt                                    <  1:1269263/150‑1 (MQ=255)
                                                                                                                       atTGCCACTGGCGCAAGGAGCGC‑GC‑AGGGGGCGGCCA‑ATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTaa                                  <  2:71853/150‑1 (MQ=35)
                                                                                                                          gCCACTGGCGCAAGGAGCGC‑GC‑AGGGGGCGGCCA‑AGCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAAc                               <  3:1394181/150‑1 (MQ=18)
                                                                                                                           ccACTGGCGCAAGGAGCGC‑GC‑AGGGGGCGGCCA‑ATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAAcgc                             <  1:144536/151‑1 (MQ=32)
                                                                                                                                ggCGCAAGGCGCGC‑GC‑AGGGGGCGGCCA‑ATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCt                        <  8:1005068/151‑1 (MQ=14)
                                                                                                                                 gcgaacggAGCGC‑GC‑TGGGGGGGGCCA‑ATCGCCGCCGCCCCCGGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTc                       <  3:538350/145‑1 (MQ=255)
                                                                                                                                      aggcgggc‑gc‑CGGGGGCGGCCA‑ATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGccc                  <  7:1814160/145‑1 (MQ=9)
                                                                                                                                       ggAGCGG‑GC‑AGGGGGCGGCCA‑ATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCca                 <  3:1849836/151‑1 (MQ=14)
                                                                                                                                           cgc‑gc‑AGGGGGCGGCCA‑ATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATGCCTGTAAAACGCGCCCTCAGCCCACATc             >  2:1692955/1‑151 (MQ=14)
                                                                                                                                            gc‑gc‑AGGGGGCGGCCA‑ATCGCCGCCGCCCCCCGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATcc            >  8:532779/1‑151 (MQ=14)
                                                                                                                                            gc‑gc‑AGGGGGCGGCCA‑ATCCCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATcc            <  5:1323846/151‑1 (MQ=14)
                                                                                                                                                   gggggCGGCCA‑ATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCAtgtg       >  8:1629076/1‑151 (MQ=31)
                                                                                                                                                    ggggCGGCCA‑ATCGCCGCCGCCCCGTGGACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTgg      <  4:1408183/151‑1 (MQ=14)
                                                                                                                                                     gggCGGCCA‑ATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTggg     >  6:1867229/1‑151 (MQ=31)
                                                                                                                                                        cggcCA‑ATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTg  <  5:1801313/151‑1 (MQ=31)
                                                                                                                                                        cggcCA‑ATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTg  <  1:756864/151‑1 (MQ=31)
                                                                                                                                                        cggcCA‑ATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTg  >  5:697738/1‑151 (MQ=31)
                                                                                                                                                        cggcCA‑AGCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTg  <  5:1871205/151‑1 (MQ=14)
                                                                                                                                                        cggcCA‑AGCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGGCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTg  <  5:1533616/151‑1 (MQ=14)
                                                                                                                                                        |                                                                                                                                                       
GCTGGAAAAGCGTGATTAATTAACGCAGAAGGCCTGGCGTAATGTCAGGCCTTTTTTTAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACATATTGCCACTGGCGCAAGGAGCGC‑GC‑AGGGGGCGGCCA‑ATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTG  >  DH1/2965749‑2966049

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑