Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH13,184,5850CA25.4% 382.6 / 59.3 181intergenic (+8/‑30)ECDH1_RS15795/ECDH1_RS15800tRNA‑Gln/tRNA‑TERM
Reads supporting (aligned to +/- strand):  ref base C (72/63);  new base A (39/7);  total (111/70)
Fisher's exact test for biased strand distribution p-value = 1.08e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 184 total aligned reads displayed.

CGAATCCCGTCGTAGCCACCAAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGA‑CGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTC  >  DH1/3184435‑3184734
                                                                                                                                                      |                                                                                                                                                       
cGACTCCCGTCGTAGCCACCAAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTTTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGCGGGTCGAATCCTCGTACCCCAGCCAATTTATTc                                                                                                                                                         <  1:885402/151‑1 (MQ=255)
         tcgtAGCCACCAAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTATAAAAGA‑ccgtt                                                                                                                                               >  3:1924791/1‑147 (MQ=255)
         ccgtACCCACCAAATTCAGAATTTATAGAATCTGAGCGCCAAATTCAATACCAATTTTATGCGTTATCGTCAAGCGGTAATCCACGGTCTTCAGATTTCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGA‑CGC‑tt                                                                                                                                              <  8:1739486/150‑1 (MQ=16)
          cgtCGCCACCATTTTATGAATCAATCTTATAAGTGCATCAGATTCAAAGCGCGTTTATTGGTGTACCGCCCAGCGCTAATGCCCCGTTTTTTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGA‑CGC‑TTa                                                                                                                                             <  7:1476665/151‑1 (MQ=12)
          cgtAGCCACCAAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTAATAAAGaaccatt                                                                                                                                               >  6:1888136/1‑145 (MQ=255)
          cgtAGCCACCAAAATCTGAATGTATCGAATATTTTCTGCAAATTCAAACCCATTTTGTTGGGGTATCTGCTAGCGGTTACGCACAGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGA‑CGC‑TTa                                                                                                                                             <  5:674707/151‑1 (MQ=25)
              gCCACCAAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATAAAAGatcctttcgtg                                                                                                                                           >  5:1892596/1‑141 (MQ=255)
                caccaAATTCTGAATTTATATAATATGTTCGGCAAATTCAAAACCAATTTTTTGTTGTATCCCCAATCTGTAAGGAAGCGGATTCTGATTCCGGCATTCCGTGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGA‑CGC‑TTACCTTGt                                                                                                                                       <  8:78227/151‑1 (MQ=25)
                caccaAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCATCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATAAAAGC‑AGC‑ATACCTTGt                                                                                                                                       >  5:1894143/1‑151 (MQ=25)
                 accaAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTATTAAAgctcgattcggagtt                                                                                                                                        >  8:1909902/1‑137 (MQ=18)
                  ccaAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGA‑CGC‑TTACCTTGTaa                                                                                                                                     <  2:1712253/151‑1 (MQ=255)
                   caAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGA‑CGC‑TTACCTTGTAAg                                                                                                                                    <  3:1944277/151‑1 (MQ=255)
                               gTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGA‑CGC‑TTACCTTGTAAGTGCACCCAGtt                                                                                                                         <  6:1686728/150‑1 (MQ=255)
                                tATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTAGAATCCTAGTACCACATCGAATTTATTAAAGA‑AAA‑TAAAATTGTAAATGAACCCAGTAgg                                                                                                                       >  8:1430681/1‑151 (MQ=18)
                                  actaaTATGTTCGGCAAATCCAAAACCAATTTGTTGGGGTATCGCCAAGCGTTACGGCACCGTATTCTGATTCCGGAATTCCGAGTTTCCATCCCGCTTACCCCAGCCAATTTATTCAACC‑CGC‑TTACCTTGTAAGTCCACCCAGTTggg                                                                                                                      <  4:430485/147‑1 (MQ=255)
                                       tatGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAAATTAAAAAAGA‑ACG‑ATACCTAGAAAGTGAACCAAGtttgcgt                                                                                                                    >  8:1509549/1‑142 (MQ=17)
                                          gTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGA‑CGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCa                                                                                                             <  7:495703/151‑1 (MQ=255)
                                          gTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTAAAGA‑AGC‑TTACCTGGTAAGTGCACCCAGTTGGGGTATCGcc                                                                                                              >  3:1614005/1‑150 (MQ=255)
                                           atcgtaaaaTTCAAAACCAATATCTAGTCTAATCTCAAATCTGTAAGGCAACTGTTGGTGATTCCGTCATTCCNAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGA‑CGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCa                                                                                                             <  2:1123804/144‑1 (MQ=19)
                                             cGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACTTTAATAAAG‑‑CGCGGTACCGTGTACTTGCTCCCAGTGGGGGTAGCTCCAAgg                                                                                                          >  3:118079/1‑150 (MQ=17)
                                             cGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGA‑CGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAg                                                                                                           <  1:1927494/150‑1 (MQ=255)
                                              ggCAAATTCAAAACCAATTTGTTGGGTTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGA‑CGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCg                                                                                                         <  5:272598/151‑1 (MQ=255)
                                               gCAAATTCAAATGCAATTTGTTGGTGTTTGTCCTAGCGGTAAGGCACCGGATTCTGATTCCGGCATTACGACGTTCGTATCCTCGTACCCCAGCCAATTTATTCAAGT‑CGC‑CTACCTTGTAAGTGCACGCAGTTGGGGTATCGCCAAGCgg                                                                                                        <  3:1109730/151‑1 (MQ=25)
                                                cAAATTCAAAACCAATTTGTTGGTGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGA‑CGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGt                                                                                                       <  3:440131/151‑1 (MQ=255)
                                                   aTTCAACACCAATATCTGGGGGTATCGCACAGCGGTAAGGCACCGGATTCTGATTCCGGCAGTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGA‑CGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAg                                                                                                    <  1:1020807/151‑1 (MQ=25)
                                                      cAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACTTTATTAAAGA‑CGG‑TTACCTTGTAAGTGATCCCTGTTTGGGTAGCGACAATCTGTAAGGcc                                                                                                 >  5:771346/1‑150 (MQ=18)
                                                          gcccggttattgGGGTATCGCCAAGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAAGTTATTCAAGA‑CGA‑tt                                                                                                                                              >  5:1350441/10‑102 (MQ=11)
                                                           ccAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTAAAGA‑AGC‑TTACCTTGAAAGTGAACCCATTTTGGGTATATACAAGATGTAAggagcagg                                                                                             >  5:227136/1‑144 (MQ=18)
                                                            cAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACTTTATTAAAGA‑CGC‑TTACCTTGTAAGTGCTCCCAGTTGGGGTAGAGGCAAGCGGTAAGGCCACGGAt                                                                                           >  5:732318/1‑151 (MQ=21)
                                                              gtttATTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGA‑CGC‑TTACCTTGTAAGTGCACCCAGt                                                                                                                          <  4:1652956/117‑1 (MQ=255)
                                                              aTTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTATACAAGATCGG‑TT‑CCGGGTAAGTGATCCGTGTTGGGGTTTCTCCAAGCTGGAAGGCCCCGGAGTc                                                                                         >  1:597667/1‑151 (MQ=2)
                                                                ttgtgggtttATCACAAAGCGTTAAGGCACCTGATTCTGATTCAGGCATTCCGAGGTTCGAATCCTCGTACCGCAGCCAATTTATTCAATA‑CGC‑TTAAATTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCt                                                                                        <  3:890371/141‑1 (MQ=25)
                                                                 tattgGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGA‑CGC‑TTACCTTGTAAGt                                                                                                                                   <  6:1369360/104‑1 (MQ=255)
                                                                    gtgggTATCGCCTAGCTGTAAGGCTCCGGATTCTGTTTCAGGCACTCCGAGGTTCGAATCCTCGTACCGCAGACTATTTATTCAAGA‑CTC‑TTAACTTGTAAGTGCACCCTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAAtt                                                                                    <  8:765455/148‑1 (MQ=25)
                                                                     gggtTATCCCCAAGCTGTACGGCACCGGATTCTGAGTCCGGCATGCCGACGGTCGAATCCTCGTACCCCAGACAATTTATTCAAGA‑CTC‑TTACATTGTAACTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTcc                                                                                  <  3:976345/151‑1 (MQ=37)
                                                                      gggTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGA‑CGC‑TTACCTTGTAAGTGCACCCAGTTgg                                                                                                                       <  5:1607928/113‑1 (MQ=255)
                                                                         tATCGCCAAGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCCTGGTTCGAATCCTCGTACCCCAGCCATTTTAGTCACGA‑CGA‑TTAGCGTGTTAGt                                                                                                                                   >  7:685980/1‑98 (MQ=1)
                                                                         tATCGCCAAGCGGTAAGGCACCGGTTTATGATACCGGCATTCCCAGGTTCGAATCCTCGTACCCCAGCCAAATTATTCAAGA‑ACA‑TTACCATGTAAGtac                                                                                                                                 >  6:796106/1‑98 (MQ=14)
                                                                         tATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTAAAGC‑AGC‑TTTCCTTGTAAGTGCACCCAGTGGGGGTAGCTCCAAGCTGTAAGGCGACGTAGTCTAATTCCGGc                                                                               >  7:419539/1‑150 (MQ=21)
                                                                           tCGCCAAGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGA‑CGC‑TTCCCATGTAAGTGCAccc                                                                                                                             >  1:317179/1‑102 (MQ=21)
                                                                            cGCCTAGCGGTAATCCACAGGATTCTGATACCGGCAGTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGA‑CGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCAtt                                                                            <  7:1904157/150‑1 (MQ=38)
                                                                               caagcgtgtcctcACCGGATGCTGACTCCGGCAGTCCTACGGTCGAATCCTCGTACCCCAGACAATTTATTCAAGC‑GGC‑TTAAAATGTAACTGCACCGAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGa                                                                        <  7:1337583/139‑1 (MQ=21)
                                                                                aaGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCa                                                                                                                                                        <  4:1105022/72‑1 (MQ=255)
                                                                                aaGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGA‑CGC‑TTACCTTGTAAGTGCACCCAGtt                                                                                                                         <  7:1522789/101‑1 (MQ=255)
                                                                                 aGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCCTGGTTCGAATCCTCGTACCCCAGCCAAGTTATTCAAGA‑CGC‑TTACCTTGAAAGTGCACCGAGTTgggtg                                                                                                                    >  7:1225244/1‑103 (MQ=17)
                                                                                 aGCGGTAAGGCACCGGTTTGTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGA‑CGC‑TTACCTTGTAAGTGCACCCAGTTGGGGt                                                                                                                    <  8:1228235/105‑1 (MQ=255)
                                                                                 aGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTAAAGA‑GCG‑GTACGGGGAGAGTGCAGCGTTTGGGGGTTGCGCCAAGCGGGAAGGCCCGGGTGTCGCTTTCCGGCCTTCCGAgt                                                                      >  1:670071/1‑150 (MQ=9)
                                                                                  ccggaaatcatacGGATTCTGTTTCCGGCTTTCCGAGGGTCGAATCCTCGTACCCCAGCCACTTTATTCAAGC‑CGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGt                                                                     <  1:90164/139‑1 (MQ=37)
                                                                                     ttAATGCTCCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCTATTTATTCAAGA‑GGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTc                                                                   <  7:106771/149‑1 (MQ=255)
                                                                                     ggactctaaaggATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGACAATTTATTCAAGA‑CGC‑TTACCTTGTAAGTGCAACCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGtt                                                                    <  4:1171946/139‑1 (MQ=38)
                                                                                       aaGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAAATTATTAAAGC‑ACC‑TTACGTGGTACGTGATCCAAGTCTGCGTTGATCCAATCTGTAAGGCGCCGGAGTCTAGTGGCGGCATTCCTAGGTTCgc                                                                 >  7:54691/1‑149 (MQ=9)
                                                                                          gCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGA‑CGC‑GTACCTTGTAAGTGCACCCAGTTGGGGTAGAGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGGTCGACTcc                                                             >  7:95825/1‑151 (MQ=255)
                                                                                          ccACCTGATGCTGACACCGGCATTCAGAGGGTCGAAGCCTCGTACCGCAGACTATTTATTAAAGC‑GGC‑GTACATTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATcc                                                             <  3:1276201/150‑1 (MQ=18)
                                                                                               ggATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTAAAGA‑AGG‑TTACGTGGAAAGTGAACCGTGTATGGGTTTAGACAATCTCTAATGCGACGTATTCTAGTTGGGTCATTCCGAgtctggattgatcgtc                                                        >  7:352639/1‑136 (MQ=9)
                                                                                                  ttCTGATTCCGGCATTCCGAGGTTCGAGTCCTCGTACCCCAGCCAATTTATACAAGC‑AGG‑GTACCTTGTAAGTGCAGCGATTTGGGGTATCGCCAAGCGGTAAGGCCCCGTATTCTACTTGGGGCATTCAGAGTCTCAACTCCTCGTcccc                                                     >  7:1464623/1‑151 (MQ=255)
                                                                                                        ttCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTAAAGA‑AGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACAGGTGTCTCTTTCCGTCATTCCCAGTTTCGAATCCTCGTCCCCCAGca                                                >  3:855389/1‑149 (MQ=25)
                                                                                                            ggCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTAAAGA‑TCC‑TTACCTAGAAAGTGCACCCATTTGGCGTATAGACAAGCGCTAAGGCAAGGGATACTAGTAAAGGCATTCAGAGTATCGAAACCGCCTAcagcatcaacaa                                            >  2:76603/1‑139 (MQ=255)
                                                                                                             ggtgtaatagcgtCGAATCCACGTAATGCAGACTATTTCTTCTAGC‑CTC‑TTAAATTTTAATTGCAATGTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTa                                          <  2:434429/139‑1 (MQ=2)
                                                                                                              caggaatagcgtCGAATCCTCCTACCGCAGACAATATATTCAAGA‑CGC‑TGACAATGCAACTGCACCGAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTaa                                         <  7:745593/140‑1 (MQ=18)
                                                                                                               aTTCCGAGGTTCGAATCCTCGTACCCCAGCCAAATTAAAAAAGC‑CCC‑GTACCTAGAAAGTTAACCAATTATGCGTATCGGCAAGCGCTAAGGCCCAGGATACAAATACCGGCATTCCAAGTATCCAATCCACCTCCCGCAGCaacaaaaa                                         >  2:1283838/1‑146 (MQ=2)
                                                                                                                 tCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATACAAGC‑CCC‑TTACCTAGTGAGTGCACCCAGTTGGGGTTTCGCCAAGCGGTAAGGCACCGGAGTCTAATTCCGGCATTCCGAGGTTCGAATGCTCGTCCCCCAGCCACAaaaaaa                                       >  5:1957635/1‑150 (MQ=21)
                                                                                                                   cGAGGTTCGAATCCTCGTACCCCAGCCAATTTATACAAGC‑ACC‑GTACCTTGTAAGTGAACCCAGTATGGGTATCTCCAAGCGGTAAGGCACAGGATTCTAATTCAGTCATTCCCAGGATCGAAACCGCCTCCCCCGGCCACAATaaaaaac                                    >  5:751360/1‑150 (MQ=255)
                                                                                                                    gAGGTTCGAATCCTCGTACCCCAGCCAATTTATTAAAGA‑CGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGTATTCTAATTCCGGCATTCCGAGGTGCGAATCCTCGTACCCCAGCCACATTAAAAAAGc                                   >  3:1635822/1‑151 (MQ=255)
                                                                                                                     aGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGA‑CGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGATCGAAGCCTCGTCCCCCAGCCACATTAAAAAAGCt                                  >  2:201138/1‑151 (MQ=255)
                                                                                                                     aGGTTCGAATCCTCGTACCCCAGCCAATTTAATAAAGA‑AGC‑TTACCTTGTAAGTGCACCAAGTGGGGGTAACTCCAAGCGGTAAGGCACAGGATTCTAGTTACGTCATTCCGAGGATCGAATCCTCGTCCCCCAGCAACATTAAAAAAGCt                                  >  4:1387106/1‑151 (MQ=255)
                                                                                                                     aGGTTCGAATCCTCGTACCCCAGCCAAATTATTAAAGA‑ACC‑TTACCTAGTAAGTGATCCAAGTATGGGTATCTGCAATCTGTAAGGCACAGTATTCTAATTCAATCATTCAGAGTATCGAAACCTCCTCCCCCAGCAAAATTAAAAAAGCt                                  >  4:917296/1‑151 (MQ=255)
                                                                                                                         tCGAATCCTCGTACCCCAGCCAATTTAATAAAGA‑AGC‑TTACCTTGTAAGTTAACCCAGTTGGGGTATCGACAAGCGCTAAGGCACAGGATTCTAATTAAGGCATTCAAAGTATCGAAACCTCGTCCCCCATCGACATAAAAAAAGCTCGCt                              >  4:402334/1‑151 (MQ=255)
                                                                                                                          cGAATCCTCGTACCCCAGCCAATTTATTAAAGA‑CGC‑TTACCTTGTAAGTGCACCGAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCCGCCACATTAAAAAAGCTCTCtt                             >  1:1386778/1‑151 (MQ=255)
                                                                                                                            tattttcttaagacAGCCATTATATTCAATT‑CTC‑TTAAATTGTAACTCCAATTTGTTTTGGTTTCGCCAATCGGTTAGGCACCGGTTTTTTATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTc                            <  8:919801/137‑1 (MQ=9)
                                                                                                                            aaTCCTCGTACCCCAGCCAATTTATTCAAGA‑CGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCg                           >  6:451213/1‑151 (MQ=255)
                                                                                                                            aaTCCTCGTACCCCAGCCAATTTATTCAAGA‑CGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCg                           >  2:806100/1‑151 (MQ=255)
                                                                                                                            aaTCCTCGTACCCCAGCCAATTTATTAAAGA‑CGC‑TTACCTTGTAATTGCACCCAGTTGGGGTAGCGCCAAGCTGTAAGGCACCGTATTCTAATTCCGGCATTCAGAGGGTCGAATCATCGTACCCCCGCAACATTAAAAAAGATCGCTTCg                           >  1:348805/1‑151 (MQ=255)
                                                                                                                            aaTCCTCGTACCCCAGCCAATTTATTAAAGA‑CGC‑TTACCTTGTAAGTGAACCAAGTTGGGGTATAGCCAAGCGGTAAGGCACAGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCg                           >  4:726370/1‑151 (MQ=255)
                                                                                                                             agccTCGTACCCCAGACACTTTCTTCAAGC‑CGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCgg                          <  1:744924/149‑1 (MQ=255)
                                                                                                                             aTCCTCGTACCCCAGCCAATTTATTCAAGA‑CGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCgg                          >  7:1659753/1‑151 (MQ=255)
                                                                                                                              tCCTCGTACCCCAGCCAATTTATTCAAGA‑CGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAATGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCATCGTACCCCAGCAACATTAAAAAAGCTCGCTTCGGc                         >  6:1163729/1‑151 (MQ=255)
                                                                                                                              tCCTCGTACCCCAGCCAATTTATTCAAGA‑CGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGc                         >  8:1588814/1‑151 (MQ=255)
                                                                                                                              tCCTCGTACCCCAGCCAATTTATTCAAGA‑CGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGc                         >  2:1860916/1‑151 (MQ=255)
                                                                                                                               ccTCGTACCCCAGCCAATTTATTCAAGA‑CGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCg                        >  7:648535/1‑151 (MQ=255)
                                                                                                                                cTCGTACCCCAGCCAATTTATTCAAGA‑CGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCg                        >  2:1276111/1‑150 (MQ=255)
                                                                                                                                cTCGTACCCCAGCCAATTTATTCAAGA‑CGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCg                        >  3:789603/1‑150 (MQ=255)
                                                                                                                                cTCGTACCCCAGCCAATTTATTCAAGA‑CGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCg                        >  8:1064336/1‑150 (MQ=255)
                                                                                                                                  cGTACCCCAGCCAATTTATTCAAGA‑CGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGc                     >  5:862792/1‑151 (MQ=255)
                                                                                                                                    tACCCCAGCCAATTTATTCAAGA‑CGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCtt                   >  1:434811/1‑151 (MQ=255)
                                                                                                                                     aCCCCAGCCAATTTATTCAAGA‑CGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCttt                  >  1:1069225/1‑151 (MQ=255)
                                                                                                                                      ccccAGCCAATTTATTCAAGA‑CGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCtttt                 >  3:1700664/1‑151 (MQ=255)
                                                                                                                                       cccAGCCACTATATTAAATA‑CTC‑TGACCAAGAAACCGCAACAAGATGTCGTATCCCCAAGCGGTAAGGCACCGGATTCCAATTCCGGGCTTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCttttt                <  1:1307118/151‑1 (MQ=16)
                                                                                                                                       accAGACAATATCTTAAAGA‑TTC‑TTAACATTTAATTGCAACTAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCttttt                <  6:1557339/150‑1 (MQ=18)
                                                                                                                                             cAATTTATTCAAGA‑CGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCtttt          >  6:990554/1‑151 (MQ=255)
                                                                                                                                             cAATATATTAAAGA‑CCC‑ATACCATGTAAGTGCACCCAGATCCGGTATCGCCAAGCCGTAAGGCACCGGATTCTAATTCCGGAATTCCTAGGATCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTAGCTTCGGCCACCATTTTGCtatt          >  6:1277836/1‑151 (MQ=18)
                                                                                                                                              aaTTTATTCAAGA‑CGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCtttt          >  5:1736772/1‑150 (MQ=255)
                                                                                                                                              aaTTTATTCAAGA‑CGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTc         >  3:143630/1‑151 (MQ=255)
                                                                                                                                                tttATTCAAGA‑CGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTg       >  6:1382417/1‑151 (MQ=255)
                                                                                                                                                 ttATTCAAGA‑CGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGc      >  1:166118/1‑151 (MQ=255)
                                                                                                                                                 ttATTCAAGA‑CGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTA‑CCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCg     >  7:1568326/1‑151 (MQ=255)
                                                                                                                                                  tATTCAAGA‑CGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCg     >  3:1275849/1‑151 (MQ=255)
                                                                                                                                                  tATTCAAGA‑CGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGAACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCg     >  3:328858/1‑151 (MQ=255)
                                                                                                                                                   aTTCAAGA‑CGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGt    >  8:686002/1‑151 (MQ=255)
                                                                                                                                                    ttCAAGA‑CGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGtt   >  8:1824703/1‑151 (MQ=255)
                                                                                                                                                     tCAAGA‑CGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGttc  >  8:816750/1‑151 (MQ=255)
                                                                                                                                                      |                                                                                                                                                       
CGAATCCCGTCGTAGCCACCAAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGA‑CGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTC  >  DH1/3184435‑3184734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑