Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH13,448,5260AC26.5% 743.7 / 107.8 421intergenic (‑86/+70)malZ/proYalpha‑glycosidase/proline‑specific permease ProY
Reads supporting (aligned to +/- strand):  ref base A (239/66);  new base C (1/109);  total (241/180)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 439 total aligned reads displayed.

AGCAGTTGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCACAACAATCCACGCGAAA  >  DH1/3448376‑3448674
                                                                                                                                                      |                                                                                                                                                    
 gCAGTTGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGAACACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGAc                                                                                                                                                     >  1:1956636/1‑151 (MQ=255)
   aGTTGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTTGGGGAGGATGAAgg                                                                                                                                                   >  1:1391707/1‑151 (MQ=255)
     ttGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACgggg                                                                                                                                                 >  5:851879/1‑151 (MQ=255)
     gtGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACgggg                                                                                                                                                 <  4:1110412/150‑1 (MQ=255)
      tGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACggggg                                                                                                                                                >  1:1533073/1‑151 (MQ=255)
        atgtttgatttgTTTAACATATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGc                                                                                                                                               <  8:439275/147‑1 (MQ=255)
        aTCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGCATATTTGCGGGAGGAGGACGGGGCCg                                                                                                                                              >  4:1949742/1‑151 (MQ=255)
           tttgctttgTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATGTGATCACGTTGGCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATAGTTGGGGGTGGAGGACGGGGGCGGAt                                                                                                                                           >  6:1498444/1‑151 (MQ=255)
           tttgctttgTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGAt                                                                                                                                           >  3:1117465/1‑151 (MQ=255)
            ttgctttgTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGAt                                                                                                                                           >  5:401645/1‑150 (MQ=255)
              gcttttTTTAACACTTGGGGGCACCGGCAGGTGCCATGCATTTAACATAATAATTCCCCTGTTATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGTATTTTTGGGGGAGGCGGCCGGGGGCGGATAAc                                                                                                                                        <  2:458140/151‑1 (MQ=255)
              gctttgTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATAACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAAc                                                                                                                                        >  5:1100138/1‑151 (MQ=255)
                tttgTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACtt                                                                                                                                      >  1:757937/1‑151 (MQ=255)
                       aacaacTGGGGGCACCGGCAGGTGACATTAATTTAACATCATATTTCCCCTGTTATGAAAATCTGATCAGTTTTCCATTGCATAATGCATGATCACTAATCATCTGATGGATTTTTGGGGGAGGCGGCCGGGGGCGGATAACTTTCGGAAt                                                                                                                               <  3:1576670/151‑1 (MQ=255)
                       aaCCAATGGGGGCACCGGCAGGTGCCATGGATTTAACATCATAATTCCCCTGTCATGAAAATCTGATCACCTTGCCATAGCCAAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAAt                                                                                                                               >  3:1320783/1‑151 (MQ=255)
                       aaCACAGGGGGGCCCCGGCAGGGGCCATGCATGTAACATCAAACTTCCCCTGTGATCAAAAGCTGATCACCTTGCCATCGCCTAATGCAGGCTCACTCATCATCTGAAGGATATTGGGGGGCGGCGGACGGGGGCGGATAACTTTCGGAAt                                                                                                                               <  6:1341502/151‑1 (MQ=255)
                        aCAACTGGGGGCACCGGCAGGTGCCATTCATTTAAAATCATAATTCCCCTGTGATCAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGTATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATa                                                                                                                              <  5:325591/151‑1 (MQ=255)
                        aCAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGGTCACTCATCATGTGAAGGATATTTGGGGGAGGAGGCCGGGGGGGGCTTACTTTAGGAAt                                                                                                                               >  2:1636090/1‑150 (MQ=255)
                         cAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAAt                                                                                                                               >  1:1746249/1‑149 (MQ=255)
                         cAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAAt                                                                                                                               >  5:622221/1‑149 (MQ=255)
                           atggggggCACCGGCATGTGCCATTAATTTAACATCATAATTCCCCTTTTATTAAAATCTGATCACCTTCCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGTTATTTGGGGGAGGAGGCCGGGGGCGGATAACTTTCGGAATAGcc                                                                                                                           <  3:255345/148‑1 (MQ=255)
                           aaTGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGcc                                                                                                                           >  2:1253281/1‑151 (MQ=255)
                           aaTGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGcc                                                                                                                           >  3:1392692/1‑151 (MQ=255)
                            aTGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAAGTTTCGGAATAgacc                                                                                                                          >  6:1692074/1‑148 (MQ=255)
                              gggcgCACGGGAAGGTGACATGAATTTAACATCAAAATTCCCCTGTGATGAAAATCTGCTCACCTTGCCATAGCCAAATGCAGCATCCCTCATCCTCTGAATGATCTTTGGGGGCGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGa                                                                                                                        <  7:63695/151‑1 (MQ=255)
                               ggggCACCGTCAGGTGCCATTAATATAAAATCATAATTCCCCTGTTATGAAAATCTGATCCCCTTCCCATAGCCTAATGCAGGATCACTCATCATCTGAAGTATATTTGGGGGAGGAGGCCGGGGGCGGATAACTTTCGGAATAGCCGGAt                                                                                                                       <  8:1131937/151‑1 (MQ=255)
                                 ggCCCCGGCAGGTGCCATGCATTTAACATAATAATTCCCCTGTTATTAAAATCTGATCCCCTTGCCATAGCCTAATGCAGTATCACTCATCATCTGAAGGATTTTTGGGGGCGGCGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCa                                                                                                                     <  2:863579/151‑1 (MQ=255)
                                 gcccccggccggTGCCATGAATTTAACATCATAATGCCCCTGTGAAGAAAATCTGAGCACCTTGCCATAGCCTAATGCACGATCACTCATCATCTGAAGCATATTGGGGGGCGGCGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCa                                                                                                                     <  7:986218/147‑1 (MQ=255)
                                       ggCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCACAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGAttt                                                                                                               >  3:386460/1‑151 (MQ=255)
                                         cAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTtcc                                                                                                             >  7:270511/1‑151 (MQ=255)
                                          aGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCCCTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATATCTTTCGGAATAGCCGGATCAGGATTtcct                                                                                                            >  1:1691244/1‑151 (MQ=255)
                                             tGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTcctcct                                                                                                         >  4:1312890/1‑151 (MQ=255)
                                                  tGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGAGATTTGGGGGCGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCCGGATGTCCTCCTGAAcc                                                                                                    >  8:1845786/1‑151 (MQ=255)
                                                   gCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCAGAGCCTAAGGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACGGTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCg                                                                                                   >  4:701267/1‑151 (MQ=255)
                                                   gCATTTAACATCATAAGTCCCCTGTGATTAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATTTTGGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAAcc                                                                                                    <  2:610734/150‑1 (MQ=255)
                                                   gCAATTAACATCATAATACCCCTGTGATGAAAACCTGATCACCATGCCATAGCCTAATGCAGTATCACGCATCATCTGAAGGATATTGGGGGGCGCCGGCCGGCGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAAcc                                                                                                    <  7:403976/150‑1 (MQ=255)
                                                     aTTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGc                                                                                                 >  4:1143221/1‑151 (MQ=255)
                                                       ttAACATCATACATCCCCTGTCATGAAAATCTGAACACCTTCCCATAGCATAATGCAGGACCACTCATCATCTGAAGGATATTTGGGGGAGGAGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCt                                                                                                <  7:1325520/150‑1 (MQ=255)
                                                       ttAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGCGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCGTGAACCGGCtt                                                                                               >  1:697758/1‑151 (MQ=255)
                                                         aaCATCATAATTCCCCTGTGACGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTAc                                                                                             >  1:1246903/1‑151 (MQ=255)
                                                           catcatAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTAc                                                                                             >  4:1648642/1‑149 (MQ=255)
                                                              catAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAAc                                                                                        >  6:42297/1‑151 (MQ=255)
                                                                tAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGc                                                                                      >  4:1184479/1‑151 (MQ=255)
                                                                    tCCCCTATGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGCACGCAAgg                                                                                  >  8:1940338/1‑151 (MQ=255)
                                                                    gccccTGTCATGAAAATCTGAACACCTTGCCATCCCCTAATGCAGCATCACCCATCATCTGAAGGATATTTGGGGGAGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAgg                                                                                  <  7:1482680/150‑1 (MQ=255)
                                                                      cccTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCTGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCTACGAt                                                                                >  2:1916137/1‑151 (MQ=255)
                                                                       ccTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGAtt                                                                               >  2:1689058/1‑151 (MQ=255)
                                                                          gttatgaAAATCTGATCACCTTCCCATACCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTa                                                                              <  8:126256/146‑1 (MQ=255)
                                                                          gggctgaAAATCTGAGCCCCTTGCCCAACCCTCATGCACGACCACGCACCCTCTGACGGATATTTGGGGGAGGCGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACt                                                                            <  1:300192/147‑1 (MQ=255)
                                                                            gatgaAAACCTCCTCACCTGGCCCTAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGCGGAGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTg                                                                           <  8:271708/150‑1 (MQ=255)
                                                                             atgaAAATCTGATCACGTTGGGATAGCCTAATGCAGGATCACTCATCATCTGATGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTGGGATTAGACGGATCAGGATTTCCTCATGAACAGGCATACAGAACTCAAGGATTTCTGGt                                                                         >  6:620893/1‑151 (MQ=255)
                                                                              tgaAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGtt                                                                        >  2:1796344/1‑151 (MQ=255)
                                                                                aaaaTCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCTGCTTACAGAACGCAAGGATTACTGGtttt                                                                      >  1:891977/1‑151 (MQ=255)
                                                                                aaaaACTCATCACCTTCCCATAGCCTAATGCAGGATCCCTCATCAGCGGACGGATAGGGGGGGGCCGAGGCCGGCGCCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGtttt                                                                      <  5:925272/151‑1 (MQ=255)
                                                                                 aaaTCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTc                                                                     >  6:71659/1‑151 (MQ=255)
                                                                                 aaaTCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTc                                                                     >  4:930905/1‑151 (MQ=255)
                                                                                 aaaTCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTc                                                                     >  7:881293/1‑151 (MQ=255)
                                                                                      tGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTcagcc                                                                 >  8:833196/1‑150 (MQ=255)
                                                                                       gATCACCTTGCCATAGCCTACTGCAGCATCACTCATCATCTGAAGGATATTTGGGGGCGGAGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTcag                                                                   <  3:202542/147‑1 (MQ=255)
                                                                                         tCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTACGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTATTCAGCCAGCt                                                             >  4:504980/1‑151 (MQ=255)
                                                                                           aCCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTgc                                                           >  6:337699/1‑150 (MQ=255)
                                                                                           aCCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTg                                                            >  3:750890/1‑150 (MQ=255)
                                                                                             cTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGCCg                                                         >  5:1429833/1‑151 (MQ=255)
                                                                                             cTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACg                                                         >  7:774330/1‑151 (MQ=255)
                                                                                             cTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACg                                                         >  7:1927811/1‑151 (MQ=255)
                                                                                               tGCCATACCCTAATGCAGGATCCCTCATCATCTTAAGGATATTTGGGGGAGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGa                                                        <  1:1865467/150‑1 (MQ=255)
                                                                                                gCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTAGTGGTTTTCAGCCAGCTGACGATc                                                      >  8:61503/1‑151 (MQ=255)
                                                                                                gCCAAAGCCTAACCCAGGCTCACTCATCATCTGAAGGATATTGGGGGGAGGCGGACGGCGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGAt                                                       <  2:1099149/150‑1 (MQ=255)
                                                                                                cccatctccTAATGCAGCCACACTCATCATCTTTAGTTTATTTGGGGGCGGGGGAGGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAAGCGGCTTACAGAACTCAAGGATTACTGGTTTTCAGCCAGCTGACCATc                                                      <  2:1551693/144‑1 (MQ=255)
                                                                                                cccataccatcctccAGCATCACTCAGCATCTGCAGGATTTGTGGGGGCGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATc                                                      <  7:1797566/137‑1 (MQ=255)
                                                                                                cccATACCCTACTGCAGGATCACTCATCATCTGAAGTATATTTGGGGGCGGCGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGAt                                                       <  6:76455/149‑1 (MQ=255)
                                                                                                  cATAGCCTACTGCAGGAGCACTCATCAGCTGAAGGCTATTTGGGGGCGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCACCCAGCTGACGATCGt                                                    <  3:1071342/151‑1 (MQ=255)
                                                                                                  cATAGCCTAATGCAGGATCACTCATCATCTCAAGGATATTTGGGGGAGGAGCACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGt                                                    <  5:1758192/151‑1 (MQ=255)
                                                                                                   aTAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTg                                                   >  1:1157410/1‑151 (MQ=255)
                                                                                                    aaGCCTAACCCACGAGCACTCATCATCTGAAGGATATTGGGGCGCGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTgg                                                  <  3:1201782/150‑1 (MQ=255)
                                                                                                     aGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTggc                                                 >  5:1286011/1‑151 (MQ=255)
                                                                                                       ccTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTggcgg                                               >  7:1241325/1‑151 (MQ=255)
                                                                                                        atAATGCAGGATCTCTAATCATCTGTAGTATTTTGGGGGGAGGTGGTCGGGGGCTGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTggcggc                                              <  6:1086870/150‑1 (MQ=255)
                                                                                                         aactGCCGGCTCACTCCTCCTCTGAAGGATCGGTGGGGGCCGCCGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGgcggcg                                             <  7:1047908/148‑1 (MQ=255)
                                                                                                          actGCAGGAGCCCTCATCCTCGGCAGGATATTTGGGGGAGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTggcggc                                              <  5:1879250/147‑1 (MQ=255)
                                                                                                          aaTGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGt                                            >  7:1667890/1‑151 (MQ=255)
                                                                                                          aaTGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGt                                            >  8:1572874/1‑151 (MQ=255)
                                                                                                          aaTGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACCATCGTGGCGGCGt                                            >  6:1218262/1‑151 (MQ=255)
                                                                                                          aaTCCAGGATCACTCCTCATCTGAAGGATATTTGGGCGCGGAGCCCGGGGCCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTggcggc                                              <  8:1688981/149‑1 (MQ=255)
                                                                                                            ggCAGGAGCACTCATCATCTGAAGGATATTGGGGGGAGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGttt                                          <  4:1409799/150‑1 (MQ=255)
                                                                                                                ggATCACGCATCATCTGAAGTATTTTTGGGGGCGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAAc                                      <  3:662111/151‑1 (MQ=255)
                                                                                                                 gATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCATGAACCGGCTTACAGAACGAAAGGATTAATGGTTTTCAGCCAGCTGACGAGCGTGGCGGCGTTTAAAc                                      >  8:35732/1‑150 (MQ=255)
                                                                                                                     aCTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATcc                                  >  3:914825/1‑150 (MQ=255)
                                                                                                                     aCTCATCATCTGAAGGATATTTGGGGGACGAGGACGGGGGCGCATAACTTTCGGAATAGCAGGATCAGGATTTCCTCCTGAAACGGATTACAGAACGCAAGGATTACTGGATTTCAGACAGCTGACGATCGTGGCGGCGTTTAAACAtac                                  >  2:334062/1‑148 (MQ=255)
                                                                                                                      cTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATcc                                  >  6:1898274/1‑149 (MQ=255)
                                                                                                                        catcatCTGAAGGATTTTTGGGGGCGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGcc                              <  6:530048/151‑1 (MQ=255)
                                                                                                                        catcatCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGcc                              >  1:511931/1‑151 (MQ=255)
                                                                                                                           catcTGACCGATTTTGGGGGGAGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCa                             <  4:1821768/149‑1 (MQ=255)
                                                                                                                             tcTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATc                          >  8:142841/1‑150 (MQ=255)
                                                                                                                                 accgATTTTTGGGGGCGGAGGCGGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACa                      <  2:761496/147‑1 (MQ=255)
                                                                                                                                 aatgATATTGGGGGGCGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACa                      <  6:1802920/147‑1 (MQ=255)
                                                                                                                                 aaGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAAc                       >  5:719414/1‑149 (MQ=255)
                                                                                                                                    gATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGca                   >  2:1740658/1‑150 (MQ=255)
                                                                                                                                            ggggAGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCACAACAATcc          <  7:525793/151‑1 (MQ=255)
                                                                                                                                             gggCGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCACAACAATCCa         <  3:524428/151‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                    
AGCAGTTGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCACAACAATCCACGCGAAA  >  DH1/3448376‑3448674

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑