Predicted mutation
evidence seq id position mutation annotation gene description
RA DH1 1,779,915 G→A E54E (GAG→GAA wzzB → chain length determinant protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH11,779,9150GA99.3% 1675.8 / ‑1.9 450E54E (GAG→GAAwzzBchain length determinant protein
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base A (220/226);  total (221/229)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

Only 100 of 457 total aligned reads displayed.

ATAATAATGTTTCTGGGCAAAACCATGACCCGGAACAGATTGATTTGATTGATTTACTAGTGCAGTTGTGGCGTGGCAAGATGACAATCATCATTTCCGTCATTGTGGCTATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAGAAATGGACGTCAACAGCAATTATCACTCAGCCCGACGTGGGGCAAATTGCTGGCTATAACAATGCCATGAATGTTATCTATGGTCAGGCTGCACCGAAAGTATCGGATTTGCAGGAGACGTTAATTGGTCGCTTCAGTTCTGCCTTCTCT  >  DH1/1779767‑1780065
                                                                                                                                                    |                                                                                                                                                      
 taataatGTTTCTGGGCAAAACCATGACCCGGAACAGATTGATTTGATTGATTTACTAGTGCAGTTGTGGCGTGGCAAGATGACAATCATCATTTCCGTCATTGTGGCTATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGaaaa                                                                                                                                                      >  5:1751084/1‑150 (MQ=255)
  aataatGTTTCTGGGCAAAACCATGACCCGGAACAGATTGATTTGATTGATTTACTAGTGCAGTTGTGGCGTGGCAAGATGACAATCATCATTTCCGTCATTGTGGCTATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAaaaa                                                                                                                                                     <  8:62305/150‑1 (MQ=255)
  aataatGTTTCTGGGCAAAACCATGACCCGGAACAGATTGATTTGATTGATTTACTAGTGCAGTTGTGGCGTGGCAAGATGACAATCATCATTTCCGTCATTGTGGCTATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAaaaa                                                                                                                                                     <  4:138556/150‑1 (MQ=255)
  aataatGTTTCTGGGCAAAACCATGACCCGGAACAGATTGATTTGATTGATTTACTAGTGCAGTTGTGGCGTGGCAAGATGACAATCATCATTTCCGTCATTGTGGCTATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAAt                                                                                                                                                    >  4:1613424/1‑151 (MQ=255)
   ataatGTTTCTGGGCAAAACCATGACCCGGAACAGATTGATTTGATTGATTTACTAGTGCAGTTGTGGCGTGGCAAGATGACAATCATCATTTCCGTCATTGTGGCTATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATg                                                                                                                                                   <  2:411028/151‑1 (MQ=255)
      atGTTTCTGGGCAAAACCATGACCCGGAACAGATTGATTTGATTGATTTACTAGTGCAGTTGTGGCGTGGCAAGATGACAATCATCATTTCCGTCATTGTGGCTATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGAc                                                                                                                                                <  5:327215/151‑1 (MQ=255)
      atGTTTCTGGGCAAAACCATGACCCGGAACAGATTGATTTGATTGATTTACTAGTGCAGTTGTGGCGTGGCAAGATGACAATCATCATTTCCGTCATTGTGGCTATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGAc                                                                                                                                                <  1:677717/151‑1 (MQ=255)
      atGTTTCTGGGCAAAACCATGACCCGGAACAGATTGATTTGATTGATTTACTAGTGCAGTTGTGGCGTGGCAAGATGACAATCATCATTTCCGTCATTGTGGCTATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGAc                                                                                                                                                >  7:1661327/1‑151 (MQ=255)
        gTTTCTGGGCAAAACCATGACCCGGAACAGATTGATTTGATTGATTTACTAGTGCAGTTGTGGCGTGGCAAGATGACAATCATCATTTCCGTCATTGTGGCTATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGACGt                                                                                                                                              >  7:1107673/1‑151 (MQ=255)
        gTTTCTGGGCAAAACCATGACCCGGAACAGATTGATTTGATTGATTTACTAGTGCAGTTGTGGCGTGGCAAGATGACAATCATCATTTCCGTCATTGTGGCTATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGACGt                                                                                                                                              >  5:162010/1‑151 (MQ=255)
         tttCTGGGCAAAACCATGACCCGGAACAGATTGATTTGATTGATTTACTAGTGCAGTTGTGGCGTGGCAAGATGACAATCATCATTTCCGTCATTGTGGATATTGCCCTGGCTATTGGAAATTTCGCAGTAGCGAAGGAAAAATGGACGt                                                                                                                                              >  6:827204/1‑150 (MQ=255)
           tCTGGGCAAAACCATGACCCGGAACAGATTGATTTGATTGATTTACTAGTGCAGTTGTGGCGTGGCAAGATGACAATCATCATTTCCGTCATTGTGGCTATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGACGTCa                                                                                                                                            >  8:538204/1‑150 (MQ=255)
           tCTGGGCAAAACCATGACCCGGAACAGATTGATTTGATTGATTTACTAGTGCAGTTGTGGCGTGGCAAGATGACAATCATCATTTCCGTCATTGTGGCTATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGACGTCa                                                                                                                                            >  7:1022970/1‑150 (MQ=255)
             tGGGCAAAACCATGACCCGGAACAGATTGATTTGAGTGATTTACTAGTGCAGTTGTGGCGTGGCAAGATGACAATCATCATTTCCGTCATTGTGGCTATTGCGCTGGCTATTGGATTTTTGGCAGTAGCGAAGGAAAAATGGACGTCAAc                                                                                                                                          >  5:1140790/1‑150 (MQ=255)
               ggCAAAACCATGACCCGGAACAGATTGATTTGATTGATTTACTAGTGCAGTTGTGGCGTGGCAAGATGACAATCATCATTTCCGTCATTGTGGCTATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGc                                                                                                                                       <  3:1465297/151‑1 (MQ=255)
                gCAAAACCATGACCCGGAACAGATTGATTTGATTGATTTACTAGTGCAGTTGTGGCGTGGCAAGATGACAATCATCATTTCCGTCATTGTGGCTATTGACCTGGCTATTCGATATTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGc                                                                                                                                       >  7:1510263/1‑150 (MQ=255)
                   aaaCCATGACCCGGAACAGATTGATTTGATTGATTTACTAGTGCAGTTGTGGCGTGGCAAGATGACAATCATCATTTCCGTCATTGTGGCTATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGCAAtt                                                                                                                                   >  5:491107/1‑151 (MQ=255)
                   aaaCCATGACCCGGAACAGATTGATTTGATTGATTTACTAGTGCAGTTGTGGCGTGGCAAGATGACAATCATCATTTCCGTCATTGTGGCTATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGCAAtt                                                                                                                                   <  2:1891468/151‑1 (MQ=255)
                    aaCCATGACCCGGAACAGATTGATTTGATTGATTTACTAGTGCAGTTGTGGCGTGGCAAGATGACAATCATCATTTCCGTCATTGTGGCTATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGCAAtt                                                                                                                                   >  3:640139/1‑150 (MQ=255)
                    aaCCATGACCCGGAACAGATTGATTTGATTGATTTACTAGTGCAGTTGTGGCGTGGCAAGATGACAATCATCATTTCCGTCATTGTGGCTATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGCAATTa                                                                                                                                  <  2:794402/151‑1 (MQ=255)
                       cATGACCCGGAACAGATTGATTTGATTGATTTACTAGTGCAGTTGTGGCGTGGCAAGATGACAATCATCATTTCCGTCATTGTGGCTATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGCAATTATcc                                                                                                                               >  8:1418805/1‑150 (MQ=255)
                       cATGACCCGGAACAGATTGATTTGATTGATTTACTAGTGCAGTTGTGGCGTGGCAAGATGACAATCATCATTTCCGTCATTGTGGCTATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGCAATTATc                                                                                                                                >  6:1019778/1‑150 (MQ=255)
                       cATGACCCGGAACAGATTGATTTGATTGATTTACTAGTGCAGTTGTGGCGTGGCAAGATGACAATCATCATTTCCGTCATTGTGGCTATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGCAATTATCa                                                                                                                               <  8:1207397/151‑1 (MQ=255)
                        aTGACCCGGAACAGATTGATTTGATTGATTTACTAGTGCAGTTGTGGCGTGGCAAGATGACAATCATCATTTCCGTCATTGTGGCTATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGCAATTATCAc                                                                                                                              >  4:1927814/1‑151 (MQ=255)
                         tGACCCGGAACAGATTGATTTGATTGATTTACTAGTGAAGTTGTTGCGTGCCAAGATGACAATCATCATTTACGTCATTGTGGCTATTGACCTGGATATTGGATATTTGGCAGTAGCGAAGGAAAAATTGACGTCAACACCAATTATCAc                                                                                                                              >  1:1126637/1‑150 (MQ=255)
                            cccGGAACAGATTGATTTGATTGATTTACTAGTGCAGTTGTGGCGTGGCAAGATGACAATCATCATTTCCGTCATTGTGGCTATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGCAATTATCACTCAg                                                                                                                          <  1:975120/151‑1 (MQ=255)
                             ccGGAACAGATTGATTTGATTGATTTACTAGTGCAGTTGTGGCGTGGCAAGATGACAATCATCATTTCCGTCATTGTGGCTATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGCAATTATCACTCAGc                                                                                                                         >  3:367371/1‑151 (MQ=255)
                              cGGAACAGATTGATTTGATTGATTTACTAGTGCAGTTGTGGCGTGGCAAGATGACAATCATCATTTCCGTCATTGTGGCTATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGCAATTATCACTCAGcc                                                                                                                        <  8:1723155/151‑1 (MQ=255)
                                gAACAGATTGATTTTATTGATTTAGTAGTGCATTTGTGGCGTTGCAAGATGACAATCATCATTTCCGTCATTGTTGCTATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGAGTTCAACAGCAATTATCACTCAGCCCg                                                                                                                      <  2:1131881/151‑1 (MQ=255)
                                gAACAGATTGATTTGATTGATTTACTAGTGCAGTTGTGGCGTTGCAAGATGACAATCATCATTTCCGTCATTGTGGCTATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGCAATTATCACTCAGCCCg                                                                                                                      <  1:787529/151‑1 (MQ=255)
                                 aaCAGATTGATTTGATTGATTTACTAGTGCAGTTGTGGCGTGGCAAGATGACAATCATCATTTCCGTCATTGTGGCTATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGCAATTATCACTCAGCCCGa                                                                                                                     <  7:664548/151‑1 (MQ=255)
                                   cAGATTGATTTGATTGATTTACTAGTGCAGTTGTGGCGTGGCAAGATGACAATCATCATTTCCGTCATTGTGGCTATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGCAATTATCACTCAGCCCGACg                                                                                                                   <  4:777602/151‑1 (MQ=255)
                                    aGATTGATTTGATTGATTTACTAGTGCAGTTGTGGCGTGGCAAGATGACAATCATCATTTCCGTCATTGTGGCTATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGCAATTATCACTCAGCCCGACGt                                                                                                                  <  2:658495/151‑1 (MQ=255)
                                      attgatttgattgatttACTAGTGCAGTTGTGGCGTGGCAAGATGACAATCATCATTTCCGTCATTGTGGCTATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGCAATTATCACTCAGACCGACGTgg                                                                                                                >  4:670157/1‑151 (MQ=255)
                                          atttgattgatttACTAGTGCAGTTGTGGCGTGGCAAGATGACAATCATCATTTCCGTCATTGTGGCTATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGCAATTATCACTCAGCCCGACGTGGGGc                                                                                                             <  6:1994461/150‑1 (MQ=255)
                                          atttgattgatttACTAGTGCAGTTGTGGCGTGGCAAGATGACAATCATCATTTCCGTCATTGTGGCTATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGCAATTATCACTCAGCCCGACGTGGGGc                                                                                                             >  2:1273274/1‑150 (MQ=255)
                                                 tgatttACTAGTGCAGTTGTGGCGTGGCAAGATGACAATCATCATTTCCGTCATTGTGGCTATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGCAATTATCACTCAGCCCGACGTGGGGCAAATTGCt                                                                                                     >  8:1601445/1‑151 (MQ=255)
                                                 tgatttACTAGTGCAGTTGTGGCGTGGCAAGATGACAATCATCATTTCCGTCATTGTGGCTATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGCAATTATCACTCAGCCCGACGTGGGGCAAATTGCt                                                                                                     >  1:1243493/1‑151 (MQ=255)
                                                  gatttACTAGTGCAGTTGTGGCGTGGCAAGATGACAATCATCATTTCCGTCATTGTGGCTATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGCAATTATCACTCAGCCCGACGTGGGGCAAATTGCTg                                                                                                    <  6:1837745/151‑1 (MQ=255)
                                                   atttACTAGTGCAGTTGTGGCGTGGCAAGATGACAATCATCATTTCCGTCATTGTGGCTATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGCAATTATCACTCAGCCCGACGTGGGGCAAATTGCTg                                                                                                    <  4:1856360/150‑1 (MQ=255)
                                                     ttACTAGTGCAGTTGTGGCGTGGCAAGATGACAATCATCATTTCCGTCATTGTGGCTATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGCAATTATCACTCAGCCCGACGTGGGGCAAATTGCTGGc                                                                                                  <  4:1523887/150‑1 (MQ=255)
                                                     ttACTAGTGCAGTTGTGGCGTGGCAAGATGACAATCATCATTTCCGTCATTGTGGCTATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGCAATTATCACTCAGCCCGACGTGGGGCAAATTGCTGGCt                                                                                                 >  6:1650846/1‑151 (MQ=255)
                                                     ttACTAGTGCAGTTGTGGCGTGGCAAGATGACAATCATCATTTCCGTCATTGTGGCTATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGCAATTATCACTCAGCCCGACGTGGGGCAAATTGCTGGCt                                                                                                 >  2:289867/1‑151 (MQ=255)
                                                         tAGTGCAGTTGTGGCGTGGCAAGATGACAATCATCATTTCCGTCATTGTGGCTATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGCAATTATCACTCAGCCCGACGTGGGGCAAATTGCTGGCtata                                                                                              >  6:1165579/1‑150 (MQ=255)
                                                          aGTGCAGTTGTGGCGTGGCAAGATGACAATCATGATTTCGGTCATTGTGGCTATTGGCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGCAATTATCACTCAGCCAGCCGTGGGGCAAATTCCTGCATATAAc                                                                                            >  6:1765110/1‑151 (MQ=255)
                                                          aGTGCAGTTGTGGCGTGGCAAGATGACAATCATCATTTCCGTCATTGTGGCTATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGCAATTATCACTCAGCCCGACGTGGGGCAAATTGCTGGCTATAAc                                                                                            >  7:331820/1‑151 (MQ=255)
                                                             gCAGTTGTGGCGTGGCAAGATGACAATCGTCATTTCCGTCATTGTGGCTATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGCAATTATCACTCAGCCCGACGTGGGGCAAATTGCTGGCTATAACAAt                                                                                         <  6:728034/151‑1 (MQ=255)
                                                             gCAGTTGTGGCGTGGCAAGATGACAATCATCATTTCCGTCATTGTGGCTATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGCAATTATCACTCAGCCCGACGTGGGGCAAATTGCTGGCTATAACaa                                                                                          <  1:367885/150‑1 (MQ=255)
                                                              cAGTTGTGGCGTGGCAAGATGACAATCATCATTTCCGTCATTGTGGCTATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGCAATTATCACTCAGCCCGACGTGGGGCAAATTGCTGGCTATAACAATg                                                                                        >  6:1587836/1‑151 (MQ=255)
                                                              cAGTTGTGGCGTGGCAAGATGACAATCATCATTTCCGTCATTGTGGCTATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGCAATTATCACTCAGCCCGACGTGGGGCAAATTGCTGGCTATAACAATg                                                                                        >  6:265475/1‑151 (MQ=255)
                                                              cAGTTGTGGCGTGGCAAGATGACAATCATCATTTCCGTCATTGTGGCTATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGCAATTATCACTCAGCCCGACGTGGGGCAAATTGCTGGCTATAACAATg                                                                                        >  6:329096/1‑151 (MQ=255)
                                                              cAGTTGTGGCGTGGCAAGATGACAATCATCATTTCCGTCATTGTGGCTATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGCAATTATCACTCAGCCCGACGTGGGGCAAATTGCTGGCTATAACAATg                                                                                        >  1:47692/1‑151 (MQ=255)
                                                               aGTTGTGGCGTGGCAAGATGTCAATCATCATTTCCGTCATTGTGGCTATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGCAATTATCACTCAGCCCGACGTGGGGCAAATTGCTGGCTATAACAATGc                                                                                       <  8:218502/151‑1 (MQ=255)
                                                               aGTTGTGGCGTGGCAAGATGACAATCATCATTTCCGTCATTGTGGCTATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGCAATTATCACTCAGCCCGACGTGGGGCAAATTGCTGGCTATAACAATGc                                                                                       <  8:443833/151‑1 (MQ=255)
                                                                  tgtgGCGTGGCAAGATGACAATCATCATTTCCGTCATTGTGGCTATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGCAATTATCACTCAGCCCGACGTGGGGCAAATTGCTGGCTATAACAATGCCa                                                                                     <  3:867427/150‑1 (MQ=255)
                                                                  tgtgGCGTGGCAAGATGACAATCATCATTTCCGTCATTGTGGCTATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGCAATTATCACTCAGCCCGACGTGGGGCAAATTGCTGGCTATAACAATGCCAt                                                                                    >  1:1479395/1‑151 (MQ=255)
                                                                   gtggcgtggcAAGATGACAATCATCATTTCCGTCATTGTGGCTATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGCAATTATCACTCAGCCCGACGTGGGGCAAATTGCTGGCTATAACAATGCCATg                                                                                   >  8:853897/1‑151 (MQ=255)
                                                                          ggcAAGATGACAATCATCATTTCCGTCATTGTGGCTATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGCAATTATCACTCAGCCCGACGTGGGGCAAATTGCTGGCTATAACAATGCCATGAATGtt                                                                             >  7:1901632/1‑150 (MQ=255)
                                                                            cAAGATGACAATCATCATTTCCGTCATTGTGGCTATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGCAATTATCACTCAGCCCGACGTGGGGCAAATTGCTGGCTATAACAATGCCATGAATGTTATc                                                                          <  7:887580/151‑1 (MQ=255)
                                                                             aaGATGACTATCTTGATTTCGGTCATTGTGGCTATTGCCCTGGCTATTGGTTATTTGGGAGTAGCGAAGGAAAAATGGACGTCAACAGGAATTATCACTCAGCCCGACGTGGGGGAAATTGCTGGCTATTACAATGCCATGAATGTTATCt                                                                         <  4:288641/151‑1 (MQ=255)
                                                                                aTGACAATCATCATTTCCGTCATTGTGGCTATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGCAATTATCACTCAGCCCGACGTGGGGCAAATTGCTGGCTATAACAATGCCATGAATGTTATCTATg                                                                      <  1:1062867/151‑1 (MQ=255)
                                                                                  gACAATCATCATTTCCGTCATTGTGGCTATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGCAATTATCACTCAGCCCGACGTGGGGCAAATTGCTGGCTATAACAATGCCATGAATGTTATCTATgg                                                                     <  1:1940680/150‑1 (MQ=255)
                                                                                  gACAATCATCATTTCCGTCATTGTGGCTATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGCAATTATCACTCAGCCCGACGTGGGGCAAATTGCTGGCTATAACAATGCCATGAATGTTATCTATGGt                                                                    >  4:554854/1‑151 (MQ=255)
                                                                                   aCAATCATCATTTCCGTCATTGTGGCTATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGCAATTATCACTCAGCCCGACGTGGGGCAAATTGCTGGCTATAACAATGCCATGAATGTTATCTATGGTc                                                                   >  4:496427/1‑151 (MQ=255)
                                                                                   aCAATCATCATTTCCGTCATTGTGGCTATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGCAATTATCACTCAGCCCGACGTGGGGCAAATTGCTGGCTATAACAATGCCATGAATGTTATCTATGGTc                                                                   >  8:182815/1‑151 (MQ=255)
                                                                                   aCAATCATCATTTCCGTCATTGTGGCTATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGCAATTATCACTCAGCCCGACGTGGGGCAAATTGCTGGCTATAACAATGCCATGAATGTTATCTATGGTc                                                                   <  7:1307852/151‑1 (MQ=255)
                                                                                    cAATCATCATTTCCGTCATTGTGGCTATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGCAATTATCACTCAGCCCGACGTGGGGCAAATTGCTGGCTATAACAATGCCATGAATGTTATCTATGGTc                                                                   >  7:431253/1‑150 (MQ=255)
                                                                                     aaTCATCATTTCCGTCATTGTGGCTATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGCAATTATCACTCAGCCCGACGTGGGGCAAATTGCTGGCTATAACAATGCCATGAATGTTATCTATGGTCAg                                                                 <  5:861784/151‑1 (MQ=255)
                                                                                        catcatTTCCGTCATTGTGGCTATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGCAATTATCACTCAGCCCGACGTGGGGCAAATTGCTGGCTATAACAATGCCATGAATGTTATCTATGGTCAGGCt                                                              <  5:1505900/151‑1 (MQ=255)
                                                                                         atcatTTCCGTCATTGTGGCTATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGCAATTATCACTCAGCCCGACGTGGGGCAAATTGCTGGCTATAACAATGCCATGAATGTTATCTATGGTCAGGCTg                                                             <  4:1707193/151‑1 (MQ=255)
                                                                                         atcatTTCCGGCATTGTGGCTATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGCAATTATCACTCAGCCCGACGTGGGGCAAATTGCTGGCTATAACAATGCCATGAATGTTATCTATGGTCAGGCTg                                                             <  6:460008/151‑1 (MQ=255)
                                                                                            atTTCCGTCATTGTGGCTATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGCAATTATCACTCAGCCCGACGTGGGGCAAATTGCTGGCTATAACAATGCCATGAATGTTATCTATGGTCAGGCTGCAc                                                          >  7:989856/1‑151 (MQ=255)
                                                                                                ccGTCATTGTGGCTATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGCAATTATCACTCAGCCCGACGTGGGGCAAATTGCTGGCTATAACAATGCCATGAATGTTATCTATGGTCAGGCTGCACCGaa                                                      <  3:1786227/151‑1 (MQ=255)
                                                                                                 cGTCATTGTGGCTATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGCAATTATCACTCAGCCCGACGTGGGGCAAATTGCTGGCTATAACAATGCCATGAATGTTATCTATGGTCAGGCTGCACCGaa                                                      >  5:581489/1‑150 (MQ=255)
                                                                                                    cATTGTGGCTATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGTAAAAATGGACGTCAACAGCAATTATCACTCAGCCCGACGTGGGGCAAATTGCTGGCTATAACAATGCCATGAATGTTATCTATGGTCAGGCTGCACCGAAAGt                                                   <  7:1956869/150‑1 (MQ=255)
                                                                                                           gCTATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGCAATTATCACTCAGCCCGACGTGGGGCAAATTGCTGGCTATAACAATGCCATGAATGTTATCTATGGTCAGGCTGCACCGAAAGTATCGGAtt                                           <  3:1571760/151‑1 (MQ=255)
                                                                                                            cTATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGCAATTATCACTCAGCCCGACGTGGGGCAAATTGCTGGCTATAACAATGCCATGAATGTTATCTATGGTCAGGCTGCACCGAAAGTATCGGAttt                                          >  7:805886/1‑151 (MQ=255)
                                                                                                             tATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGCAATTATCACTCAGCCCGACGTGGGGCAAATTGCTGGCTATAACAATGCCATGAATGTTATCTATGGTCAGGCTGCACCGAAAGTATCGGATTTg                                         >  3:1324192/1‑151 (MQ=255)
                                                                                                             tATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGCAATTATCACTCAGCCCGACGTGGGGCAAATTGCTGGCTATAACAATGCCATGAATGTTATCTATGGTCAGGCTGCACCGAAAGTATCGGATTTg                                         >  3:723704/1‑151 (MQ=255)
                                                                                                              aTTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGCAATTATCACTCAGCCCGACGTGGGGCAAATTGCTGGCTATAACAATGCCATGAATGTTATCTATGGTCAGGCTGCACCGAAAGTATCGGAttt                                          <  3:1927655/149‑1 (MQ=255)
                                                                                                              aTTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGCAATTATCACTCAGCCCGACGTGGGGCAAATTGCTGGCTATAACAATGCCATGAATGTTATCTATGGTCAGGCTGCACCGAAAGTATCGGAttt                                          <  1:1658595/149‑1 (MQ=255)
                                                                                                              aTTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGCAATTATCACTCAGCCCGACGTGGGGCAAATTGCTGGCTATAACAATGCCATGAATGTTATCTATGGTCAGGCTGCACCGAAAGTATCGGAttt                                          <  1:1403717/149‑1 (MQ=255)
                                                                                                                tGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGCAATTATCACTCAGCCCGACGTGGGGCAAATTGCTGGCTATAACAATGCCATGAATGTTATCTATGGTCAGGCTGCACCGAAAGTATCGGATTTGCa                                       <  4:1009579/150‑1 (MQ=255)
                                                                                                                tGCCCTCGCTATTGGATATTTAGCAGTAGCGAAGGAAAAATGGAAGTCAACAAAAAATATCACTCAGAACCACGTGGGGCAAATTGCTGGCTATAACAAACGCATGAATGTTATCTATGGACAGGCTGCACCTAAAGTATCGGATTTGc                                        >  8:706847/1‑149 (MQ=255)
                                                                                                                   ccTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGCAATTATCACTCAGCCCGACGTGGGGCAAATTGCTGGCTATAACAATGCCATGAATGTTATCTATGGTCAGGCTGCACCGAAAGTATCGGATTTGCAgg                                     >  8:1090074/1‑149 (MQ=255)
                                                                                                                    cTGGCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGCAATTATCACTCAGCCCGACGTGGGGCAAATTGCTGGCTATAACAATGCCATGAATGTTATCTATGGTCAGGCTGCACCGAAAGTATCGGATTTGCAgg                                     >  1:10105/1‑148 (MQ=255)
                                                                                                                      ggCTATTGGATATTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGCAATTATCACTCAGCCCGACGTGGGGCAAATTGCTGGCTATAACAATGCCATGAATGTTATCTATGGTCAGGCTGCACCGAAAGTATCGGATTTGCAGGAGAc                                 <  3:307413/150‑1 (MQ=255)
                                                                                                                           ttGGATATTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGCAATTATCACTCAGCCCGACGTGGGGCAAATTGCTGGCTATAACAATGCCATGAATGTTATCTATGGTCAGGCTGCACCGAAAGTATCGGATTTGCAGGAGACGTTAAt                           >  3:1227068/1‑151 (MQ=255)
                                                                                                                           ttGGATATTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGCAATTATCACTCAGCCCGACGTGGGGCAAATTGCTGGCTATAACAATGCCATGAATGTTATCTATGGTCAGGCTGCACCGAAAGTATCGGATTTGCAGGAGACGTTAAt                           >  6:656678/1‑151 (MQ=255)
                                                                                                                             ggATATTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGCAATTATCACTCAGCCCGACGTGGGGCAAATTGCTGGCTATAACAATGCCATGAATGTTATCTATGGTCAGGCTGCACCGAAAGTATCGGATTTGCAGGAGACGTTAAtt                          <  3:1685313/150‑1 (MQ=255)
                                                                                                                              gATATTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGCAATTATCACTCAGCCCGACGTGGGGCAAATTGCTGGCTATAACAATGCCATGAATGTTATCTATGGTCAGGCTGCACCGAAAGTATCGGATTTGCAGGAGACGTTAATTgg                        <  2:964726/151‑1 (MQ=255)
                                                                                                                              gATATTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGCAATTATCACTCAGCACGACGTGGGGCAAATTGCTGGCTATAACAATGCCATGAATGTTATCTATGGTCAGGCTGCACCGAAAGTATCGGATTTGCAGGAGACGTTAATTgt                        >  2:159580/1‑150 (MQ=255)
                                                                                                                                tatTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGCAATTATCACTCAGCCCGACGTGGGGCAAATTGCTGGCTATAACAATGCCATGAATGTTATCTATGGTCAGGCTGCACCGAAAGTATCGGTTTTGCAGGAGACGTTAATTGGTc                      >  6:1303808/1‑151 (MQ=255)
                                                                                                                                tatTTGGCAGTAGCGAAGGAAAAATGGACGTCAACAGCAATTATCACTCAGCCCGACGTGGGGCAAATTGCTGGCTATAACAATGCCATGAATGTTATCTATGGTCAGGCTGCACCGAAAGTATCGGATTTGCAGGAGACGTTAATTGGTc                      >  1:1777583/1‑151 (MQ=255)
                                                                                                                                      gCAGTAGCGAAGGAAAAATGGACGTCAACAGCAATTATCACTCAGCCCGACGTGGGGCAAATTGCTGGCTATAACAATGCCATGAATGTTATCTATGGTCAGGCTGCACCGAAAGTATCGGATTTGCAGGAGACGTTAATTGGTCGCTTCa                <  6:126266/151‑1 (MQ=255)
                                                                                                                                           aGCGAAGGAAAAATGGACGTCAACAGCAATTATCACTCAGCCCGACGTGGGGCAAATTGCTGGCTATAACAATGCCATGAATGTTATCTATGGTCAGGCTGCACCGAAAGTATCGGATTTGCAGGAGACGTTAATTGGTCGCTTCAGTTCt           <  8:1337977/151‑1 (MQ=255)
                                                                                                                                           aGCGAAGGAAAAATGGACGTCAACAGCAATTATCACTCAGCCCGACGTGGGGCAAATTGCTGGCTATAACAATGCCATGAATGTTATCTATGGTCAGGCTGCACCGAAAGTATCGGATTTGCAGGAGACGTTAATTGGTCGCTTCAGTTCt           >  4:1268440/1‑151 (MQ=255)
                                                                                                                                           aGCGAAGGAAAAATGGACGTCAACAGCAATTATCACTCAGCCCGACGTGGGGCAAATTGCTGGCTATAACAATGCCATGAATGTTATCTATGGTCAGGCTGCACCGAAAGTATCGGATTTGCAGGAGACGTTAATTGGTCGCTTCAGTTCt           >  5:1370728/1‑151 (MQ=255)
                                                                                                                                             cGAAGGAAAAATGGACGTCAACAGCAATTATCACTCAGCCCGACGTGGGGGAAATTGCGGGCTATAACAATGCCATGAATGTTATCTATGGTCAGGCTGCACCGAAAGTATCGGATTTGCAGGAGACGTTAATTGGTCGCTTCAGTTCTGc         <  7:1192661/151‑1 (MQ=255)
                                                                                                                                                aGGAAAAATGGACGTCAACAGCAATTATCACTCAGCCCGACGTGGGGCAAATTGCTGGCTATAACAATGCCATGAATGTTATCTATGGTCAGGCTGCACCGAAAGTATCGGATTTGCAGGAGACGTTAATTGGTCGCTTCAGTTCTGCCtt      >  5:1018565/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                      
ATAATAATGTTTCTGGGCAAAACCATGACCCGGAACAGATTGATTTGATTGATTTACTAGTGCAGTTGTGGCGTGGCAAGATGACAATCATCATTTCCGTCATTGTGGCTATTGCCCTGGCTATTGGATATTTGGCAGTAGCGAAGGAGAAATGGACGTCAACAGCAATTATCACTCAGCCCGACGTGGGGCAAATTGCTGGCTATAACAATGCCATGAATGTTATCTATGGTCAGGCTGCACCGAAAGTATCGGATTTGCAGGAGACGTTAATTGGTCGCTTCAGTTCTGCCTTCTCT  >  DH1/1779767‑1780065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: