Predicted mutation
evidence seq id position mutation annotation gene description
RA DH1 1,885,611 G→A W125* (TGG→TAG)  pgsA → CDP‑diacylglycerol‑‑glycerol‑3‑phosphate 3‑phosphatidyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH11,885,6110GA98.3% 1505.1 / 6.2 412W125* (TGG→TAG) pgsACDP‑diacylglycerol‑‑glycerol‑3‑phosphate 3‑phosphatidyltransferase
Reads supporting (aligned to +/- strand):  ref base G (2/5);  new base A (207/197);  total (209/203)
Fisher's exact test for biased strand distribution p-value = 2.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

Only 100 of 412 total aligned reads displayed.

GCTATCGCCATGGTGCTGGTAACCGAGCATTATCACAGCTGGTGGGTGACATTACCGGCGGCAACGATGATCGCCCGTGAAATTATTATTTCTGCGCTACGCGAATGGATGGCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTGGATTGGGAAAGTGAAAACCACTGCCCAGATGGTGGCGTTGGCATGGCTGCTGTGGCGTCCGAACATTTGGGTTGAGTACGCCGGTATTGCACTTTTCTTTGTGGCTGCGGTACTGACTCTGTGGTCAATGTTGCAATATTTGAGCGC  >  DH1/1885463‑1885758
                                                                                                                                                    |                                                                                                                                                   
   aTCGCCATGGTGCTGGTAACCGAGCATTATCACAGCTGGTGGGTGACATTACCGGCGGCAACGATGATCGCCCGTGAAATTATTATTTCTGCGCTACGCGAATGGATGGCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTg                                                                                                                                                <  6:1407456/151‑1 (MQ=255)
   aTCGCCATGGTGCTGGTAACCGAGCATTATCACAGCTGGTGGGTGACATTACCGGCGGCAACGATGATCGCCCGTGAAATTATTATTTCTGCGCTACGCGAATGGATGGCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTg                                                                                                                                                <  2:829990/151‑1 (MQ=255)
     cGCCATGGTGCTGGTAACCGAGCATTATCACAGCTGGTGGGTGACATTACCGGCGGCAACGATGATCGCCCGTGAAATTATTATTTCTGCGCTACGCGAATGGATGGCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTcgg                                                                                                                                              >  1:1906388/1‑151 (MQ=255)
        cATGGTGCTGGTAACCGAGCATTATCACAGCTGGTGGGTGACATTACCGGCGGCAACGATGATCGCCCGTGAAATTATTATTTCTGCGCTACGCGAATGGATGGCGGAGTTGGTTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGaa                                                                                                                                            >  1:628800/1‑150 (MQ=255)
        cATGGTGCTGGTAACCGAGCATTATCACAGCTGGTGGGTGACATTACCGGCGGCAACGATGATCGCCCGTGAAATTATTATTTCTGCGCTACGCGAATGGATGGCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGaa                                                                                                                                            >  4:1761727/1‑150 (MQ=255)
         aTGGTGCTGGTAACCGAGCATTATCACAGCTGGTGGGTGACATTACCGGCGGCAACGATGATCGCCCGTGAAATTATTATTTCTGCGCTACGCGAATGGATGGCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAg                                                                                                                                          <  4:1449534/151‑1 (MQ=255)
           ggTGCTGGTAACCGAGCATTATCACAGCTGGTGGGTGACATTACCGGCGGCAACGATGATCGCCCGTGAAATTATTATTTCTGCGCTACGCGAATGGATGGCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGt                                                                                                                                         <  2:1570020/150‑1 (MQ=255)
                 ggTAACCGAGCATTATCACAGCTGGTGGGTGACATTACCGGCGGCAACGATGATCGCCCGTGAAATTATTATTTCTGCGCTACGCGAATGGATGGCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGaaaa                                                                                                                                    <  5:454286/149‑1 (MQ=255)
                 ggTAACCGAGCATTATCACAGCTGGTGGGTGACATTACCGGCGGCAACGATGATCGCCCGTGAAATTATTATTTCTGCGCTACGCGAATGGATGGCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAAcc                                                                                                                                  >  4:610701/1‑151 (MQ=255)
                 ggTAACCGAGCATTATCACAGCTGGTGGGTGACATTACCGGCGGCAACGATGATCGCCCGTGAAATTATTATTTCTGCGCTACGCGAATGGATGGCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAAcc                                                                                                                                  <  5:1168983/151‑1 (MQ=255)
                    aaCCGAGCATTATCACAGCTGGTGGGTGACATTACCGGCGGCAACGATGATCGCCCGTGAAATTATTATTTCTGCGCTACGCGAATGGATGGCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCAc                                                                                                                                <  4:1663101/150‑1 (MQ=255)
                    aaCCGAGCATTATCACAGCTGGTGGGTGACATTACCGGCGGCAACGATGATCGCCCGTGAAATTATTATTTCTGCGCTACGCGAATGGATGGCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCAc                                                                                                                                <  3:1481947/150‑1 (MQ=255)
                     aCCGAGCATTATCACAGCTGGTGGGTGACATTACCGGCGGCAACGATGATCGCCCGTGAAATTATTATTTCTGCGCTACGCGAATGGATGGCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTg                                                                                                                              <  4:811627/151‑1 (MQ=255)
                     aCCGAGCATTATCACAGCTGGTGGGTGACATTACCGGCGGCAACGATGATCGCCCGTGAAATTATTATTTCTGCGCTACGCGAATGGATGGCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTg                                                                                                                              <  3:1862989/151‑1 (MQ=255)
                        gAGCATTATCACAGCTGGTGGGTGACATTACCGGCGGCAACGATGATCGCCCGTGAAATTATTATTTCTGCGCTACGCGAATGGATGGCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTGccc                                                                                                                           >  2:1963363/1‑151 (MQ=255)
                           cATTATCACAGCTGGTGGGTGACATTACCGGCGGCAACGATGATCGCCCGTGAAATTATTATTTCTGCGCTACGCGAATGGATGGCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTGccc                                                                                                                           >  2:64151/1‑148 (MQ=255)
                           cATTATCACAGCTGGTGGGTGACATTACCGGCGGCAACGATGATCGCCCGTGAAATTATTATTTCTGCGCTACGCGAATGGATGGCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTGccc                                                                                                                           >  3:444505/1‑148 (MQ=255)
                            aTTATCACAGCTGGTGGGTGACATTAGCGGCGGAAACGATGATCGCCCGTGAAATTATTATTTCTGCGCTACGCGAATGGATGGCGGAGTTGGGTAAACGCAGTAGAGTGGCCGTCTCCTAGATTGGTAAAGTGAAAACCACTGCCCAGAt                                                                                                                       <  2:1266618/151‑1 (MQ=255)
                            aTTATCACAGCTGGTGGGTGACATTACCGGCGGCAACGATGATCGCCCGTGAAATTATTATTTCTGCGCTACGCGAATGGATGGCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTGCCCAGAt                                                                                                                       <  6:1944292/151‑1 (MQ=255)
                               aTCACAGCTGGTGGGTGACATTACCGGCGGCAACGATGATCGCCCGTGAAATTATTATTTCTGCGCTACGCGAATGGATGGCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTGGATTGGGAAAGTGAAAACCACTGCCCAGAtggt                                                                                                                    <  2:182427/151‑1 (MQ=255)
                                tCACAGCTGGTGGGTGACATTACCGGCGGCAACGATGATCGCCCGTGAAATTATTATTTCTGCGCTACGCGAATGGATGGCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTGCCCAGAtggtg                                                                                                                   >  3:544775/1‑151 (MQ=255)
                                 cacaGCTGGTGGGTGACATTACCGGCGGCAACGATGATCGCCCGTGAAATTATTATTTCTGCGCTACGCGAATGGATGGCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTGGATTGGGAAAGTGAAAACCACTGCCCAGAtggtg                                                                                                                   <  8:809531/150‑1 (MQ=255)
                                 cacaGCTGGTGGGTGACATTACCGGCGGCAACGATGATCGCCCGTGAAAATATTATTTCTGCGCTACGCGAATGGATGGCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTGCCCAGAtggtgg                                                                                                                  >  7:567710/1‑151 (MQ=255)
                                 cacaGCTGGTGGATGACATTACCGGCGGCAACGATGATCGCCCGTGAAATTATTATTTCTGCGCTACGCGAATGGATGGCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTGCCCAGAtggtgg                                                                                                                  >  8:1535761/1‑151 (MQ=255)
                                  acaGCTGGTGGGTGACATTACCGGCGGCAACGATGATCGCCCGTGAAATTATTATTTCTGCGCTACGCGAATGGATGGCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTGCCCAGATGGTGGc                                                                                                                 >  6:692204/1‑151 (MQ=255)
                                      cTGGTGGGTGACATTACCGGCGGCAACGATGATCGCCCGTGAAATTATTATTTCTGCGCTACGCGAATGGATGGCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTGCCCAGATGGTGGCGTTg                                                                                                             >  3:310723/1‑151 (MQ=255)
                                         gtgggtgACATTACCGGCGGCAACGATGATCGCCCGTGAAATTATTATTTCTGCGCTACGCGAATGGATGGCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTGCCCAGATGGTGGCGTTGGCa                                                                                                          <  3:1591510/151‑1 (MQ=255)
                                         gtgggtgACATTACCGGCGGCAACGATGATCGCCCGTGAAATTATTATTTCTGCGCTACGCGAATGGATGGCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTGCCCAGATGGTGGCGTTGGCa                                                                                                          <  5:309050/151‑1 (MQ=255)
                                          ggggtgACATTACCGGCGGCAACGATGATCGCCCGTGAAATTATTATTTCTGCGCTACGCGAATGGATGGCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTGCCCAGATGGTGGCGTTGGCAt                                                                                                         <  4:189253/150‑1 (MQ=255)
                                           gggtgACATTACCGGCGGCAACGATGATCGCCCGTGAAATTATTATTTCTGCGCTACGCGAATGGATGGCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTGCCCAGATGGTGGCGTTGGCATg                                                                                                        <  3:1104181/151‑1 (MQ=255)
                                           gggtgACATTACCGGCGGCAACGATGATCGCCCGTGAAATTATTATTTCTGCGCTACGCGAATGGATGGCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTGCCCAGATGGTGGCGTTGGCATg                                                                                                        >  4:232609/1‑151 (MQ=255)
                                            ggtgACATTACCGGCGGCAACGATGATCGCCCGTGAAATTATTATTTCTGCGCTACGCGAATGGATGGCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTGCCCAGATGGTGGCGTTGGCATgg                                                                                                       <  3:1932150/151‑1 (MQ=255)
                                                  aTTACCGGCGGCAACGATGATCGCCCGTGAAATTATTATTTCTGCGCTACGCGAATGGATGGCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTGCCCAGATGGTGGCGTTGGCATGGctgctg                                                                                                 >  8:1822431/1‑151 (MQ=255)
                                                  aTTACCGGCGGCAACGATGATCGCCCGTGAAATTATTATTTCTGCGCTACGCGAATGGATGGCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTGCCCAGATGGTGGCGTTGGCATGGctgctg                                                                                                 <  1:490247/151‑1 (MQ=255)
                                                    tACCGGCGGCAACGATGATCGCCCGTGAAATTATTATTTCTGCGCTACGCGAATGGATGGCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTGCCCAGATGGTGGCGTTGGCATGGCTGCtgt                                                                                                <  8:1267885/150‑1 (MQ=255)
                                                     aCCGGCGGCAACGATGATCGCCCGTGAAATTATTATTTCTGCGCTACGCGAATGGATGGCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTGCCCAGATGGTGGCGTTGGCATGGCTGCtgtg                                                                                               <  1:1441074/150‑1 (MQ=255)
                                                     aCCGGCGGCAACGATGATCGCCCGTGAAATTATTATTTCTGCGCTACGCGAATGGATGGCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTGCCCAGATGGTGGCGTTGGCATGGCTGCTGTgg                                                                                              >  8:289693/1‑151 (MQ=255)
                                                       cggcggCAACGATGATCGCCCGTGAAATTATTATTTCTGCGCTACGCGAATGGATGGCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTGCCCAGATGGTGGCGTTGGCATGGCTGCTGTGGCg                                                                                            >  2:1232127/1‑151 (MQ=255)
                                                       cggcggCAACGATGATCGCCCGTGAAATTATTATTTCTGCGCTACGCGAATGGATGGCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTGCCCAGATGGTGGCGTTGGCATGGCTGCTGTGGCg                                                                                            >  4:1179740/1‑151 (MQ=255)
                                                        ggcggcAACGATGATCGCCCGTGAAATTATTATTTCTGCGCTACGCGAATGGATGGCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTGCCCAGATCGTGGCGTTGGCATGGCTGCTGTGGCgg                                                                                           >  8:1636322/1‑150 (MQ=255)
                                                         gcggcAACGATGATCGCCCGTGAAATTATTATTTCTGCGCTACGCGAATGGATGGCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTGCCCAGATGGTGGCGTTGGCATGGCTGCTGTGGCGTc                                                                                          <  1:1015499/151‑1 (MQ=255)
                                                              aaCGATGATCGCCCGTGAAATTATTATTTCTGCGCTACGCGAATGGATGGCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTGCCCAGATGGTGGCGTTGGCATGGCTGCTGTGGCGTCCGAAc                                                                                     >  2:11039/1‑151 (MQ=255)
                                                               aCGATGATCGCCCGTGAAATTATTATTTCTGCGCTACGCGAATGGATGGCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTGCCCAGATGGTGGCGTTGGCATGGCTGCTGTGGCGTCCGAACa                                                                                    <  5:586831/151‑1 (MQ=255)
                                                                cGATGATCGCCCGTGAAATTATTATTTCTGCGCTACGCGAATGGATGCCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACAACTGCCCAGATGGTGGCGTTGGCATGGCTGCTGTGGCGTCCGAACAt                                                                                   >  4:424383/1‑151 (MQ=255)
                                                                 gatgatCGCCCGTGAAATTATTATTTCTGCGCTACGCGAATGGATGGCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTGCCCAGATGGTGGCGTTGGCATGGCTGCTGTGGCGTCCGAACAtt                                                                                  <  7:1529414/151‑1 (MQ=255)
                                                                  atgatCGCCCGTGAAATTATTATTTCTGCGCTACGCGAATGGATGGCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTGCCCAGATGGTGGCGTTGGCATGGCTGCTGTGGCGTCCGAACAttt                                                                                 >  8:735075/1‑151 (MQ=255)
                                                                   tgatCGCCCGTGAAATTATTATTTCTGCGCTACGCGAATGGATGGCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTGCCCAGATGGTGGCGTTGGCATGGCTGCTGTGGCGTCCGAACATTTg                                                                                >  7:82847/1‑151 (MQ=255)
                                                                     atCGCCCGTGAAATTATTATTTCTGCGCTACGCGAATGGATGGCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTGCCCAGATGGTGGCGTTGGCATGGCTGCTGTGGCGTCCGAACATTTggg                                                                              >  4:942796/1‑151 (MQ=255)
                                                                       ggCCCGTGAAATTATTATTTCTGCGCTACGCGAATGGATGGCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTGCCCAGATGGTGGCGTTGGCATGGCTGCTGTGGCGTCCGAACATTTGGGtt                                                                            <  4:709706/150‑1 (MQ=255)
                                                                       cGCCCGTGAAATTATTATTTCTGCGCTACGCGAATGGATGGCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTGCCCAGATGGTGGCGTTGGCATGGCTGCTGTGGCGTCCGAACATTTGGGt                                                                             <  7:1463965/150‑1 (MQ=255)
                                                                        gCCCGTGAAATTATTATTTCTGCGCTACGCGAATGGATGGCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTGCCCAGATGGTGGCGTTGGCATGGCTGCTGTGGCGTCCGAACATTTGGGTTg                                                                           <  1:1269875/151‑1 (MQ=255)
                                                                         cccGTGAAATTATTATTTCTGCGCTACGCGAATGGATGGCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTGCCCAGATGGTGGCGTTGGCATGGCTGCTGTGGCGTCCGAACATTTGGGTTg                                                                           >  6:484387/1‑150 (MQ=255)
                                                                           cGTGAAATTATTATTTCTGCGCTACGCGAATGGATGGCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTGCCCAGATGGTGGCGTTGGCATGGCTGCTGTGGCGTCCGAACATTTGGGTTGAGt                                                                        >  8:1454442/1‑151 (MQ=255)
                                                                              gAAATTATTATTTCTGCGCTACGCGAATGGATGGCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTGCCCAGATGGTGGCGTTGGCATGGCTGCTGTGGCGTCCGAACATTTGGGTTGAGTAc                                                                      <  6:808179/150‑1 (MQ=255)
                                                                              gAAATTATTATTTCTGCGCTACGCGAATGGATGGCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTGCCCAGATGGTGGCGTTGGCATGGCTGCTGTGGCGTCCGAACATTTGGGTTGAGTACg                                                                     <  5:135544/151‑1 (MQ=255)
                                                                              gAAATTATTATTTCTGCGCTACGCGAATGGATGGCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTGCCCAGATGGTGGCGTTGGCATGGCTGCTGTGGCGTCCGAACATTTGGGTTGAGTACg                                                                     >  5:186568/1‑151 (MQ=255)
                                                                                   tattattTCTGCGCTACGCGAATGGATGGCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTGCCCAGATGGTGGCGTTGGCATGGCTGCTGTGGCGTCCGAACATTTGGGTTGAGTACGCCgg                                                                 <  7:1373629/150‑1 (MQ=255)
                                                                                   tattattTCTGCGCTACGCGAATGGATGGCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTGCCCAGATGGTGGCGTTGGCATGGCTGCTGTGGCGTCCGAACATTTGGGTTGAGTACGCCgg                                                                 <  7:964747/150‑1 (MQ=255)
                                                                                   tattattTCTGCGCTACGCGAATGGATGGCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTGCCCAGATGGTGGCGTTGGCATGGCTGCTGTGGCGTCCGAACATTTGGGTTGAGTACGCCgg                                                                 <  1:1549669/150‑1 (MQ=255)
                                                                                       attTCTGCGCTACGCGAATGGATGGCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTGCCCAGATGGTGGCGTTGGCATGGCTGCTGTGGCGTCCGAACATTTGGGTTGAGTACGCCGGTAtt                                                             <  3:1348368/150‑1 (MQ=255)
                                                                                         ttCTGCGCTACGCGAATGGATGGCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAACACCACTGCCCAGATGGTGGCGTTGGCATGGCTGCTGTGGCGTCCGAACATTTGGGTTGAGTACGCCGGTATTGc                                                           >  1:167653/1‑150 (MQ=255)
                                                                                         ttCTGCGCTACGCGAATGGATGGCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTGCCCAGATGGTGGCGTTGGCATGGCTGCTGTGGCGTCCGAAGATTTGGGTTGAGTACGCCGGTATTGcc                                                          >  6:1806556/1‑150 (MQ=255)
                                                                                             gcgcTACGCGAATGGATGGCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTGGATTGGGAAAGTGAAAACCACTGCCCAGATGGTGGCGTTGGCATGGCTGCTGTGGCGTCCGAACATTTGGGTTGAGTACGCCGGTATTGCACtt                                                       <  3:1220489/150‑1 (MQ=255)
                                                                                             gcgcTACGCGAATGGATGGCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTGCCCAGATGGTGGCGTTGGCATGGCTGCTGTGGCGTCCGAACATTTGGGTTGAGTACGCCGGTATTGCACtt                                                       <  7:1328989/150‑1 (MQ=255)
                                                                                              cgcTACGCGAATGGATGGCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTGCCCAGATGGTGGCGTTGGCATGGCTGCTGTGGCGTCCGAACATTTGGGTTGAGTACGCCGGTATTGCACtttt                                                     <  8:480087/151‑1 (MQ=255)
                                                                                                 tACGCGAATGGATGGCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTGCCCAGATGGTGGCGTTGGCATGGCTGCTGTGGCGTCCGAACATTTGGGTTGAGTACGCCGGTATTGCACTTTTCtt                                                  >  5:93187/1‑151 (MQ=255)
                                                                                                    gcgAATGGATGGCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTGGATTGGGAAAGTGAAAACCACTGCCCAGATGGTGGCGTTGGCATGGCTGCTGTGGCGTCCGAACATTTGGGTTGAGTACGCCGGTATTGCACTTTTCTTtgt                                               <  8:899286/151‑1 (MQ=255)
                                                                                                    gcgAATGGATGGCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTGCCCAGATGGTGGCGTTGGCATGGCTGCTGTGGCGTCCGAACATTTGGGTTGAGTACGCCGGTATTGCACTTTTCTTtgt                                               >  1:921234/1‑151 (MQ=255)
                                                                                                     cgAATGGATGCCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTGCCCAGATGGTGGCGTTGGCATGGCTGCTGTGGCGTCCGAACATTTGGGTTGAGTACGCCGGTATTGCACTTTTCTTtgtg                                              <  2:360434/151‑1 (MQ=255)
                                                                                                      gAATGGATGGCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTGCCCAGATGGTGGCGTTGGCATGGCTGCTGTGGCGTCCGAACATTTGGGTTGAGTACGCCGGTATTGCACTTTTCTTTGTgg                                             >  6:1690828/1‑151 (MQ=255)
                                                                                                      gAATGGATGGCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTGCCCAGATGGTGGCGTTGGCATGGCTGCTGTGGCGTCCGAACATTTGGGTTGAGTACGCCGGTATTGCACTTTTCTTTGTgg                                             >  2:530854/1‑151 (MQ=255)
                                                                                                       aaTGGATGGCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTGCCCAGATGGTGGCGTTGGCATGGCTGCTGTGGCGTCCGAACATTTGGGTTGAGTACGCCGGTATTGCACTTTTCTTTGTGGc                                            >  8:120166/1‑151 (MQ=255)
                                                                                                         tggatggCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTGCCCAGATGGTGGCGTTGGCATGGCTGCTGTGGCGTCCGAACATTTGGGTTGAGTACGCCGGTATTGCACTTTTCTTTGTGGCTg                                          >  4:1335497/1‑151 (MQ=255)
                                                                                                          ggatggCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTGCCCAGATGGTGGCGTTGGCATGGCTGCTGTGGCGTCCGAACATTTGGGTTGAGTACGCCGGTATTGCACTTTTCTTTGTGGCTg                                          <  7:1818364/150‑1 (MQ=255)
                                                                                                          ggatggCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTGCCCAGATGGTGGCGTTGGCATGGCTGCTGTGGCGTCCGAACATTTGGGTTGAGTACGCCGGTATTGCACTTTTCTTTGTGGCTGc                                         >  8:658116/1‑151 (MQ=255)
                                                                                                          ggatggCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTGCCCAGATGGTGGCGTTGGCATGGCTGCTGTGGCGTCCGAACATTTGGGTTGAGTACGCCGGTATTGCACTTTTCTTTGTGGCTGc                                         >  6:318807/1‑151 (MQ=255)
                                                                                                           gatggCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTGCCCAGATGGTGGCGTTGGCATGGCTGCTGTGGCGTCCGAACATTTGGGTTGAGTACGCCGGTATTGCACTTTTCTTTGTGGCTGCg                                        <  6:1099409/151‑1 (MQ=255)
                                                                                                              ggCGGAGTTGGGTAAACGCAGTAGCGTGGCCGGCTCCTAGATTGGGAAAGTGAAAACCACTGCCCAGATGGTGGCGTTGGGATGGCTGCGGTGGCGTCCGAACATTTGGGTTGAGTACGCCGGTATTGCACTTTTCTTTGTGGCTGCGGTa                                     <  1:1179542/151‑1 (MQ=255)
                                                                                                               gCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTGCCCAGATGGTGGCGTTGGCATGGCTGCTGTGGCGTCCGAACATTTGGGTTGAGTACGCCGGTATTGCACTTTTCTTTGTGGCTGCGGTAc                                    <  6:1293118/151‑1 (MQ=255)
                                                                                                                cGGAGTGGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTGCCCAGATGGTGGCGTTGGCATGGCTGCGGTGGCGTCCGAACATTTGGGTTGAGTACGCCGGTATTGCACTTTTCTTTGTGGCTGCGGTACt                                   <  4:360542/151‑1 (MQ=255)
                                                                                                                  gAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTGCGCAGATGGTGGCGTTGGCATGGCTGCTGTGGCGTCCGAACATTTGGGTTGAGTACGCCGGTATTGCACTTTTCTTTGTGGCTGCGGTACTg                                  >  4:315588/1‑150 (MQ=255)
                                                                                                                  gAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTGCCCAGATGGTGGCGTTGGCATGGCTGCTGTGGCGTCCGAACATTTGGGTTGAGTACGCCGGTATTGCACTTTTCTTTGTGGCTGCGGTACTGa                                 <  8:632957/151‑1 (MQ=255)
                                                                                                                   aGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTGCCCAGATGGTGGCGTTGGCATGGCTGCTGTGGCGTCCGAACATTTGGGTTGAGTACGCCGGTATTGCACTTTTCTTTGTGGCTGCGGTACTGAc                                >  4:1539739/1‑151 (MQ=255)
                                                                                                                       gggTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTGCCCAGATGGTGGCGTTGGCATGGCTGCTGTGGCGTCCGAACATTTGGGTTGAGTACGCCGGTATTGCACTTTTCTTTGTGGCTGCGGTACTGACTCtg                            >  7:4768/1‑151 (MQ=255)
                                                                                                                       gggTAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTGCCCAGATGGTGGCGTTGGCATGGCTGCTGTGGCGTCCGAACATTTCGGTTGAGTACGCCGGTATTGCACTTTTCTTTGTGGCTGCGGTACTGACTCtg                            >  3:939753/1‑151 (MQ=255)
                                                                                                                          tAAACGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTGCCCAGATGGTGGCGTTGGCATGGCTGCTGTGGCGTCCGAACATTTGGGTTGAGTACGCCGGTATTGCTCTTTTCTTTGTGGCTGCGGTACTGACTCTGTgg                         >  2:1864938/1‑151 (MQ=255)
                                                                                                                           aaaCGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTGCCCAGATGGTGGCGTTGGCATGGCTGCTGTGGCGTCCGAACATTTGGGTTGAGTACGCCGGTATTGCACTTTTCTTTGTGGCTGCGGTACTGACTCTGTgg                         <  7:1148831/150‑1 (MQ=255)
                                                                                                                              cGCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTGCCCAGATGGTGGCGTTGGCATGGCTGCTGTGGCGTCCGAACATTTGGGTTGAGTACGCCGGTATTGCACTTTTCTTTGTGGCTGCGGTACTGACTCTGTGGTCaa                     <  2:132211/151‑1 (MQ=255)
                                                                                                                               gCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTGCCCAGATGGTGGCGTTGGCATGGCTGCTGTGGCGTCCGAACATTTGGGTTGAGTACGCCGGTATTGCACTTTTCTTTGTGGCTGCGGTACTGACTCTGTGGTCAAt                    <  4:979495/151‑1 (MQ=255)
                                                                                                                               gCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTGCCCAGATGGTGGCGTTGGCATGGCTGCTGTGGCGTCCGAACATTTGGGTTGAGTACGCCGGTATTGCACTTTTCTTTGTGGCTGCGGTACTGACTCTGTGGTCAAt                    >  6:1995278/1‑151 (MQ=255)
                                                                                                                               gCAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTGCCCAGATGGTGGCGTTGGCATGGCTGCTGTGGCGTCCGAACATTTGGGTTGAGTACGCCGGTATTGCACTTTTCTTTGTGGCTGCGGTACTGACTCTGTGGTCAAt                    <  1:340445/151‑1 (MQ=255)
                                                                                                                                cAGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAAGCAGTGGCCAGATGGTGGCGTTGGCATGGCTGCTGTGGCGTCCGAACATTTGTGTTGAGTACGCCGGTATTGCACTTTTCTTTGTGGCTGCGGTAGTGACTCTGTGGTCAATg                   <  1:1371963/151‑1 (MQ=255)
                                                                                                                                 aGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTGCCCAGATGGTGGCGTTGGCATGGCTGCTGTGGCGTCCGAACATTTGGGTTGAGTACGCCGGTATTGCACTTTTCTTTGTGGCTGCGGTACTGACTCTGTGGTCAATGt                  >  3:7401/1‑151 (MQ=255)
                                                                                                                                 aGTAGCGTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTGCCCAGATGGTGGCGTTGGCATGGCTGCTGTGGCGTCCGAACATTTGGGTTGAGTACGCCGGTATTGCACTTTTCTTTGTGGCTGCGGTACTGACTCTGTGGTCAATGt                  >  2:1053060/1‑151 (MQ=255)
                                                                                                                                       gTGGCCGTCTCCTAGATTGGGAAAGTGAAAACCACTGCCCAGATGGTGGCGTTGGCATGGCTGCTGTGGCGTCCGAACATTTGGGTTGAGTACGCCGGTATTGCACTTTTCTTTGTGGCTGCGGTACTGACTCTGTGGTCAATGTTGCAat            <  7:1938596/151‑1 (MQ=255)
                                                                                                                                               ctcCTAGATTGGGAAAGTGAAAACCACTGCCCAGATGGTGGCGTTGGCATGGCTGCTGTGGCGTCCGAACATTTGGGTTGAGTACGCCGGTATTGCACTTTTCTTTGTGGCTGCGGTACTGACTCTGTGGTCAATGTTGCAATATTTGAgc    >  3:674657/1‑151 (MQ=255)
                                                                                                                                                 ccTAGATTGGGAAAGTGAAAACCACTGCCCAGATGGTGGCGTTGGCATGGCTGCTGTGGCGTCCGAACATTTGGGTTGAGTACGCCGGTATTGCACTTTTCTTTGTGGCTGCGGTACTGACTCTGTGGTCAATGTTGCAATATTTGAgc    <  5:1726153/149‑1 (MQ=255)
                                                                                                                                                 ccTAGATTGGGAAAGTGAAAACCACTGCCCAGATGGTGGCGTTGGCATGGCTGCTGTGGCGTCCGAACATTTGGGTTGAGTACGCCGGTATTGCACTTTTCTTTGTGGCTGCGGTACTGACTCTGTGGTCAATGTTGCAATATTTGAgc    <  8:569261/149‑1 (MQ=255)
                                                                                                                                                 ccTAGATTGGGAAAGTGAAAACCACTGCCCAGATGGTGGCGTTGGCATGGCTGCTGTGGCGTCCGAACATTTGGGTTGAGTACGCCGGTATTGCACTTTTCTTTGTGGCTGCGGTACTGACTCTGTGGTCAATGTTGCAATATTTGAgc    <  7:252595/149‑1 (MQ=255)
                                                                                                                                                 ccTAGATTGGGAAAGTGAAAACCACTGCCCAGATGGTGGCGTTGGCATGGCTGCTGTGGAGTCCGAACATTTGGGTTGAGTACGCCGGTATTGCACTTTTCTTTGTGGCTGCGTTACTGACTCTGTGGTCAATGTTGCAATATTTGAgc    <  5:765821/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GCTATCGCCATGGTGCTGGTAACCGAGCATTATCACAGCTGGTGGGTGACATTACCGGCGGCAACGATGATCGCCCGTGAAATTATTATTTCTGCGCTACGCGAATGGATGGCGGAGTTGGGTAAACGCAGTAGCGTGGCCGTCTCCTGGATTGGGAAAGTGAAAACCACTGCCCAGATGGTGGCGTTGGCATGGCTGCTGTGGCGTCCGAACATTTGGGTTGAGTACGCCGGTATTGCACTTTTCTTTGTGGCTGCGGTACTGACTCTGTGGTCAATGTTGCAATATTTGAGCGC  >  DH1/1885463‑1885758

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑