Predicted mutation
evidence seq id position mutation annotation gene description
RA DH1 1,985,603 G→T D121Y (GAT→TAT)  yoaA → ATP‑dependent helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH11,985,6030GT98.2% 1409.1 / 8.7 379D121Y (GAT→TAT) yoaAATP‑dependent helicase
Reads supporting (aligned to +/- strand):  ref base G (3/4);  new base T (179/193);  total (182/197)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 379 total aligned reads displayed.

CTCAAAAGCGTTGCAGGATCAGCTCTACAGCCGCGATTTGCCAACAGTCTCAAAGGCATTGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCGATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCGTGGCGGAAGATTCACAGGCGTGGCCGCTGGTCACCAGCACCAACGACAACTGTCTTG  >  DH1/1985455‑1985750
                                                                                                                                                    |                                                                                                                                                   
cTCAAAAGCGTTGCAGGATCAGCTCTACAGCCGCGATTTGCCAACAGTCTCAAAGGCATTGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTAt                                                                                                                                                   >  7:1597271/1‑151 (MQ=255)
 tCAAAAGCGTTGCAGGATCAGCTCTACAGCCGCGATTTGCCAACAGTCTCAAAGGCATTGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATc                                                                                                                                                  >  4:475067/1‑151 (MQ=255)
  cAAAAGCGTTGCAGGATCAGCTCTACAGCCGCGATTTGCCAACAGTCTCAAAGGCATTGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATc                                                                                                                                                  <  2:1672929/150‑1 (MQ=255)
  cAAAAGCGTTGCAGGATCAGCTCTACAGCCGCGATTTGCCAACAGTCTCAAAGGCATTGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCt                                                                                                                                                 >  2:458056/1‑151 (MQ=255)
  cAAAAGCGTTGCAGGATCAGCTCTACAGCCGCGATTTGCCAACACTCTCAAAGGCATTGAAATATACGTGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTAt                                                                                                                                                   <  8:10964/149‑1 (MQ=255)
   aaaaGCGTTGCAGGATCAGCTCTACAGCCGCGATTTGCCAACAGTCTCAAAGGCATTGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTg                                                                                                                                                >  8:500136/1‑151 (MQ=255)
      aGCGTTGCAGGATCAGCTCTACAGCCGCGATTTGCCAACAGGCTCAAAGGCATTGAAATATACGGGCAACGTGGCGCGGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCg                                                                                                                                             <  1:889793/151‑1 (MQ=255)
        cGTTGCAGGATCAGCTCTACAGCCGCGATTTGCCAACAGTCTCAAAGGCATTGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGt                                                                                                                                           <  5:293156/151‑1 (MQ=255)
        cGTTGCAGGATCAGCTCTACAGCCGCGATTTGCCAACAGTCTCAAAGGCATTGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGt                                                                                                                                           >  7:1163748/1‑151 (MQ=255)
         gTTGCAGGATCAGCTCTACAGCCGCGATTTGCCAACAGTCTCAAAGGCATTGAAATATACGGGCAACGTGGCGCTGGTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACCTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCggg                                                                                                                                           >  2:280580/1‑149 (MQ=255)
           tGCAGGATCAGCTCTACAGCCGCGATTTGCCAACAGTCTCAAAGGCATTGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTAc                                                                                                                                         <  7:599853/150‑1 (MQ=255)
                 aTCAGCTCTACAGCCGCGATTTGCCAACAGTCTCAAAGGCAATGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTATGCCTGCAACGTCTCCAACAGCAGGCGCTGCAGGGGGGCTATCTGGAGGTACAAATCtt                                                                                                                                  >  3:162312/1‑151 (MQ=255)
                  tCAGCTCTACAGCCGCGATTTGCCAACAGTCTCAAAGGCATTGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCtt                                                                                                                                  >  5:940191/1‑150 (MQ=255)
                   cAGCTCTACAGCCGCGATTTGCCAACAGTCTGAAAGGCATTGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTa                                                                                                                                 >  4:1265968/1‑150 (MQ=255)
                    aGCTCTACAGCCGCGATTTGCCAACAGTCTCAAAGGCATTGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTa                                                                                                                                 >  5:802370/1‑149 (MQ=255)
                      ctctACAGCCGCGATTTGCCAACAGTCTCAAAGGCATTGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCg                                                                                                                             >  6:1818574/1‑151 (MQ=255)
                      ctctACAGCCGCGATTTGCCAACAGTCTCAAAGGCATTGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCg                                                                                                                             >  5:1197988/1‑151 (MQ=255)
                        ctACAGCCGCGATTTGCCAACAGTCTCAAAGGCATTGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGAt                                                                                                                           >  8:1327438/1‑151 (MQ=255)
                          aCAGCCGCGATTTGCCAACAGTCTCAAAGGCATTGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGAtgt                                                                                                                         <  3:636832/151‑1 (MQ=255)
                          aCAGCCGCGATTTGCCAACAGTCTCAAAGGCATTGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGAtgt                                                                                                                         >  5:107929/1‑151 (MQ=255)
                          aCAGCCGCGATTTGCCAACAGTCTCAAAGGCATTGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGAtg                                                                                                                          <  4:573675/150‑1 (MQ=255)
                           cAGCCGCGATTTGCCAACAGTCTCAAAGGCATTGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGAtgtg                                                                                                                        <  1:1941894/151‑1 (MQ=255)
                             gCCGCGATTTGCCAACAGTCTCAAAGGCATTGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGAt                                                                                                                      >  3:1263426/1‑151 (MQ=255)
                              ccGCGATTTGCCAACAGTCTCAAAGGCATTGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATc                                                                                                                     >  1:1025861/1‑151 (MQ=255)
                                 ggATTTGCCAACAGTGTCAAAGGCATTGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCctg                                                                                                                  <  6:1279136/150‑1 (MQ=255)
                                  gATTTGCCAACAGTCTCAAAGGCATTGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCctgc                                                                                                                 >  4:1676989/1‑151 (MQ=255)
                                        ccAACAGTCTCAAAGGCATTGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCGATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCt                                                                                                           >  3:1313864/1‑151 (MQ=255)
                                        ccAACAGTCTCAAAGGCATTGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCACTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCGATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCt                                                                                                           <  6:353694/151‑1 (MQ=255)
                                           aCAGTCTCAAAGGCATTGAAATATACTGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTAGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGAGCCTGCTGCGCTCCt                                                                                                        <  6:1387068/151‑1 (MQ=255)
                                           aCAGTCTCAAAGGCATTGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCt                                                                                                        <  5:1655104/151‑1 (MQ=255)
                                              gTCTCAAAGGCATTGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGt                                                                                                     >  4:1552545/1‑151 (MQ=255)
                                                  cAAAGGCATTGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTa                                                                                                  >  7:1316979/1‑150 (MQ=255)
                                                       gCATTGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCGTGCTGCGCTCCTGGTCTAATCaaa                                                                                            <  3:1755272/151‑1 (MQ=255)
                                                         aTTGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAAGAGCAGGGGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACa                                                                                          <  4:1618022/151‑1 (MQ=255)
                                                         aTTGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACa                                                                                          <  7:85767/151‑1 (MQ=255)
                                                           tGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCGTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAg                                                                                         <  8:649720/150‑1 (MQ=255)
                                                           tGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGt                                                                                        >  7:46778/1‑151 (MQ=255)
                                                            gAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTc                                                                                       >  5:615803/1‑151 (MQ=255)
                                                             aaaTATACGTGCAACGTGTCGGTGCTGAAAGGTCGCTCAAACTACCTCTGCCTCTATCGTCTCGAAGAGCAGTCGGTGGCGGGTGGCTATGTGCCGTTAGAAATCTTTAGCGATGTGATGCTGCTGCGCTCCTGGTCTAATCTAAGAGTc                                                                                       <  8:746648/150‑1 (MQ=255)
                                                              aaTATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCg                                                                                      >  6:1972706/1‑150 (MQ=255)
                                                              aaTATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCg                                                                                      >  3:342951/1‑150 (MQ=255)
                                                               atatAGGGGCCACGGCGCGCTGCTGAACGGGCGCGCAATCTACCTCTGCCTCGATCGTCTGGAACAGCAGGCGGTGGCGGTGGGCGATCTGCCGGTACAAATCTTAACCGATGGGAGCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGAt                                                                                    <  3:723178/151‑1 (MQ=255)
                                                               atatACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGAt                                                                                    >  2:1081948/1‑151 (MQ=255)
                                                                   aCGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGt                                                                                 <  5:1844065/150‑1 (MQ=255)
                                                                    cGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTg                                                                                >  4:1428319/1‑150 (MQ=255)
                                                                       gCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGatat                                                                            >  1:551027/1‑151 (MQ=255)
                                                                       gCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGatat                                                                            >  6:623790/1‑151 (MQ=255)
                                                                         aaCGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATc                                                                           >  3:1921109/1‑150 (MQ=255)
                                                                         aaCGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCa                                                                          <  8:1712245/151‑1 (MQ=255)
                                                                            gTGGCGCTGCTGAAAGGGCGCTTAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCCTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCa                                                                       <  6:802716/151‑1 (MQ=255)
                                                                            gTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCa                                                                       <  8:1357456/151‑1 (MQ=255)
                                                                              ggCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCAcc                                                                     >  8:1889238/1‑151 (MQ=255)
                                                                                cgcTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTg                                                                   <  3:102536/151‑1 (MQ=255)
                                                                                cgcTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTg                                                                   <  8:330969/151‑1 (MQ=255)
                                                                                 gctgctGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGc                                                                  >  4:1335513/1‑151 (MQ=255)
                                                                                  ctgctgAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCg                                                                 >  3:1810305/1‑151 (MQ=255)
                                                                                   tgctgAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGt                                                                >  8:224698/1‑151 (MQ=255)
                                                                                    gctgAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTc                                                               >  8:1436812/1‑151 (MQ=255)
                                                                                    gctgAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTc                                                               <  7:1066267/151‑1 (MQ=255)
                                                                                     ctgAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTc                                                               >  1:476829/1‑150 (MQ=255)
                                                                                     ctgAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTc                                                               >  2:1524441/1‑150 (MQ=255)
                                                                                      ngAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTATTCAAACAGTCGATGGTGATATCAGCACCTGCGTc                                                               >  5:1919061/2‑149 (MQ=255)
                                                                                       gAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGc                                                            <  8:1027122/151‑1 (MQ=255)
                                                                                       gAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGc                                                            <  6:1752579/151‑1 (MQ=255)
                                                                                       gAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGGCGATGGTGATATCAGCACCTGCGTCAg                                                             <  4:108350/150‑1 (MQ=255)
                                                                                        aaaGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCg                                                           <  2:747398/151‑1 (MQ=255)
                                                                                        aaaGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCg                                                           <  3:1178658/151‑1 (MQ=255)
                                                                                               gcTCAAAGTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCGTGGCgg                                                     >  1:1618783/1‑150 (MQ=255)
                                                                                                cTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCGTGGCGGa                                                    >  6:380264/1‑150 (MQ=255)
                                                                                                  cAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCGTGGCGGa                                                    >  2:1233891/1‑148 (MQ=255)
                                                                                                  cAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCGTGGCGGAAGa                                                 <  7:21501/151‑1 (MQ=255)
                                                                                                     aCTACCTCTGCCTCGAACGGCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGGCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCGTGGCGGAAGATTc                                              <  6:55026/151‑1 (MQ=255)
                                                                                                        aCCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCGTGGCGGAAGATTcac                                            <  7:1859737/150‑1 (MQ=255)
                                                                                                         ccTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCGTGGCGGAAGATTCACAg                                          <  4:777481/151‑1 (MQ=255)
                                                                                                         ccTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCGTGGCGGAAGATTCACAg                                          <  3:970035/151‑1 (MQ=255)
                                                                                                         ccTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCGTGGCGGAAGATTCACAg                                          <  1:664686/151‑1 (MQ=255)
                                                                                                            ctGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCGTGGCGGAAGATTCACAGGCg                                       >  7:947919/1‑151 (MQ=255)
                                                                                                             tGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCGTGGCGGAAGATTCACAGGCGt                                      >  6:448686/1‑151 (MQ=255)
                                                                                                             tGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCGTGGCGGAAGATTCACAGGCGt                                      >  4:1937517/1‑151 (MQ=255)
                                                                                                              gCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCGTGGCGGAAGATTCACAGGCGt                                      <  3:524670/150‑1 (MQ=255)
                                                                                                              gCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCGTGGCGGAAGATTCACAGGCGt                                      <  1:142373/150‑1 (MQ=255)
                                                                                                                  cGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCGTGGCGGAAGATTCACAGGCGTGGCCg                                 <  3:467049/151‑1 (MQ=255)
                                                                                                                          tcGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCGTGGCGGAAGATTCACAGGCGTGGCCGCTGGTCAc                         >  1:1749867/1‑151 (MQ=255)
                                                                                                                           cGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCGTGGCGGAAGATTCACAGGCGTGGCCGCTGGTc                           <  3:748232/148‑1 (MQ=255)
                                                                                                                           cGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCGTGGCGGAAGATTCACAGGCGTGGCCGCTGGTCAcc                        >  7:1712461/1‑151 (MQ=255)
                                                                                                                           cGAACAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCGTGGCGGAAGATTCACAGGCGTGGCCGCTGGTCAcc                        >  5:1411941/1‑151 (MQ=255)
                                                                                                                             aaCAGCAGGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCGTGGCGGAAGATTCACAGGCGTGGCCGCTGGTCACCAg                      <  1:1163740/151‑1 (MQ=255)
                                                                                                                               cagcagGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCGTGGCGGAAGATTCACAGGCGTGGCCGCTGGTCACCAGCa                    <  1:124810/151‑1 (MQ=255)
                                                                                                                                agcagGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCGTGGCGGAAGATTCACAGGCGTGGCCGCTGGTCACCAGCAc                   <  2:1485386/151‑1 (MQ=255)
                                                                                                                                 gcagGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCGTGGCGGAAGATTCACAGGCGTGGCCGCTGGTCACCAGCAcc                  >  7:347337/1‑151 (MQ=255)
                                                                                                                                 gcagGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCGTGGCGGAAGATTCACAGGCGTGGCCGCTGGTCACCAGCAcc                  <  1:1497403/151‑1 (MQ=255)
                                                                                                                                  cagGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCGTGGCGGAAGATTCACAGGCGTGGCCGCTGGTCACCAGCACCa                 <  4:710113/151‑1 (MQ=255)
                                                                                                                                   agGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCGTGGCGGAAGATTCACAGGCGTGGCCGCTGGTCACCAGCACCaa                <  5:897268/151‑1 (MQ=255)
                                                                                                                                   agGCGCTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCGTGGCGGAAGATTCACAGGCGTGGCCGCTGGTCACCAGCACCaa                <  3:1432333/151‑1 (MQ=255)
                                                                                                                                     gcgcTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCGTGGCGGAAGATTCACAGGCGTGGCCGCTGGTCACCAGCACCaaag              <  5:1775556/151‑3 (MQ=255)
                                                                                                                                      cgcTGGCGGGGGGCGATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCGTGGCGGAAGATTCACAGGCGTGGCCGCTGGTCACCAGCACCAACg              >  6:103463/1‑150 (MQ=255)
                                                                                                                                       gcTGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCCGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCGTGGCGGAAGATTCACAGGCGTGGCCGCTGGTCACCAGCACCAACGAc            >  7:1529563/1‑151 (MQ=255)
                                                                                                                                         tGGCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCGTGGCGGAAGATTCACAGGCGTGGCCGCTGGTCACCAGCACCAACGACa           >  6:1156375/1‑150 (MQ=255)
                                                                                                                                          ggCGGGGGGCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCGTGGCGGAAGATTCACAGGCGTGGCCGCTGGTCACCAGCACCAACGACaa          <  4:1814932/150‑1 (MQ=255)
                                                                                                                                              gggggCTATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCGTGGCGGAAGATTCACAGGCGTGGCCGCTGGTCACCAGCACCAACGACAACTGTc     >  2:1956108/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CTCAAAAGCGTTGCAGGATCAGCTCTACAGCCGCGATTTGCCAACAGTCTCAAAGGCATTGAAATATACGGGCAACGTGGCGCTGCTGAAAGGGCGCTCAAACTACCTCTGCCTCGAACGTCTCGAACAGCAGGCGCTGGCGGGGGGCGATCTGCCGGTACAAATCTTAAGCGATGTGATCCTGCTGCGCTCCTGGTCTAATCAAACAGTCGATGGTGATATCAGCACCTGCGTCAGCGTGGCGGAAGATTCACAGGCGTGGCCGCTGGTCACCAGCACCAACGACAACTGTCTTG  >  DH1/1985455‑1985750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑