Predicted mutation
evidence seq id position mutation annotation gene description
RA DH1 2,019,390 T→A I381N (ATC→AAC)  ydjK → MFS sugar transporter membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH12,019,3900TA98.0% 1513.0 / 7.4 407I381N (ATC→AAC) ydjKMFS sugar transporter membrane protein
Reads supporting (aligned to +/- strand):  ref base T (6/2);  new base A (217/182);  total (223/184)
Fisher's exact test for biased strand distribution p-value = 3.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01

Only 100 of 410 total aligned reads displayed.

GGTGTGGGGCTATTAATCCTGATTGCGGTGCTCGGATATATCTATTCACTGCAAACCAGTATGTTGCTCATAACGTTGATTGGTTTCTTCCTGATTACTTTCGTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAATCTGGCCGACAGAGGCCAAACTCCGTGGCTCCGGTCTGGCGAATGCGGTAGGGCGAATCAGTGGTATTGCCGCACCTTATGCCGTTGCAGTGCTGCTCAGTAGTTATGGCGTTACGGGAGTCTTTATTCTTCTGGGGGCGGTTTCAAT  >  DH1/2019242‑2019537
                                                                                                                                                    |                                                                                                                                                   
ggTGTGGGGCTATTAATCCTGATTGCGGTGCTCGGATATATCTATTCACTGCAAACCAGTATGTTGCTCATAACGTTGATTGGTTTCTTCCTGATTACTTTCGTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACt                                                                                                                                                   <  7:754737/151‑1 (MQ=255)
 gtgtGGGGCTATTAATCCTGATTGCGGTGCTCGGATATATCTATTCACTGCAAACCAGTATGTTGCTCATAACGTTGATTGGTTTCTTCCTGATTACTTTCGTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTg                                                                                                                                                  <  3:709946/151‑1 (MQ=255)
       ggCTATTAATCCTGATTGCGGTGCTCGGATATATCTATTCACTGCAAACCAGTATGTTGCTCATAACGTTGATTGGTTTCTTCCTGATTACTTTCGTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGAc                                                                                                                                            <  4:277881/151‑1 (MQ=255)
        gCTATTAATCCTGATTGCGGTGCTCGGATATATCTATTCACTGCAAACCAGTATGTTGCTCATAACGTTGATTGGTTTCTTCCTGATTACTTTCGTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTTAAAACTGGCCCAc                                                                                                                                            >  4:154302/1‑150 (MQ=255)
         cTATTAATCCTGATTGCGGTGCTCGGATATATCTATTCACTGCAAACCAGTATGTTGCTCATAACGTTGATTGGTTTCTTCCTGATTACTTTCGTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGAc                                                                                                                                            >  5:1605877/1‑149 (MQ=255)
          tATTAATCCTGATTGCGGTGCTCGGATATATCTATTCACTGCAAACCAGTATGTTGCTCATAACGTTGATTGGTTTCTTCCTGATTACTTTCGTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGAc                                                                                                                                            >  3:100252/1‑148 (MQ=255)
          tATTAATCCTGATTGCGGTGCTCGGATATATCTATTCACTGCAAACCAGTATGTTGCTCATAACGTTGATTGGTTTCTTCCTGATTACTTTCGTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGAc                                                                                                                                            >  1:763165/1‑148 (MQ=255)
           aTTAATCCTGATTGCGGTGCTCGGATATATCTATTCACTGCAAACCAGTATGTTGCTCATAACGTTGATTGGTTTCTTCCTGATTACTTTCGTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACaga                                                                                                                                         <  2:629074/150‑1 (MQ=255)
           aTTAATCCTGATTGCGGTGCTCGGATATATCTATTCACTGCAAACCAGTATGTTGCTCATAACGTTGATTGGTTTCTTCCTGATTACTTTCGTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACag                                                                                                                                          <  6:222557/149‑1 (MQ=255)
              aaTCCTGATTGCGGTGCTCGGATATATCTATTCACTGCAAACCAGTATGTTGCTCATAACGTTGATTGGTTTCTTCCTGATTACTTTCGTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGcc                                                                                                                                     <  7:1543730/151‑1 (MQ=255)
                   tGATTGCGGTGCTCGGATATATCTATTCACTGCAAACCAGTATGTTGCTCATAACGTTGATTGGTTTCTTCCTGATTACTTTCGTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACt                                                                                                                                >  4:622809/1‑151 (MQ=255)
                    gATTGCGGTGCTCGGATATATCTATTCACTGCAAACCAGTATGTTGCTCATAACGTTGATTGGTTTCTTCCTGATTACTTTCGTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAAc                                                                                                                                 <  1:20965/149‑1 (MQ=255)
                    gATTGCGGTGCTCGGATATATCTATTCACTGCAAACCAGTATGTTGCTCATAACGTTGATTGGTTTCTTCCTGATTACTTTCGTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTc                                                                                                                               >  7:625139/1‑151 (MQ=255)
                    gATTGCGGTGCTCGGATATATCTATTCACTGCAAACCAGTATGTTGCTCATAACGGTGATTGGTTTCTTCCTGATTACTTTCGTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTc                                                                                                                               <  1:693166/151‑1 (MQ=255)
                    gATTGCGATGCTCGGATATATCTATTCACTGCAAACCAGTATGTTGCTCATAACGTTGATTGGTTTCTTCCTGATTACTTTCGTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACt                                                                                                                                <  6:810165/150‑1 (MQ=255)
                     aTTGCGGTGCTCGGATATATCTATTCACTGCAAACCAGTATGTTGCTCATAACGTTGATTGGTTTCTTCCTGATTACTTTCGTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGCGGCCAAACTcc                                                                                                                              >  4:47865/1‑151 (MQ=255)
                      ttGCGGTGCTCGGATATATCTATTCACTGCAAACCAGTATGTTGCTCATAACGTTGATTGGTTTCTTCCTGATTACTTTCGTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCCg                                                                                                                             >  5:319358/1‑151 (MQ=255)
                      ttGCGGTGCTCGGATATATCTATTCACTGCAAACCAGTATGTTGCTCATAACGTTGATTGGTTTCTTCCTGATTACTTTCGTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCCg                                                                                                                             >  1:1288660/1‑151 (MQ=255)
                       tGCGGTGCTCGGATATATCTATTCACTGCAAACCAGTATGTTGCTCATAACGTTGATTGGTTTCTTCCTGATTACTTTCGTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCCGt                                                                                                                            >  6:1200578/1‑151 (MQ=255)
                       tGCGGTGCTCGGATATATCTATTCACTGCAAACCAGTATGTTGCTCATAACGTTGATTGGTTTCTTCCTGATTACTTTCGTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCCGt                                                                                                                            >  4:55753/1‑151 (MQ=255)
                        gCGGTGCTCGGATATATCTATTCACTGCAAACCAGTATGTTGCTCATAACGTTGATTGGTTTCTTCCTGATTACTTTCGTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCCGTg                                                                                                                           >  6:1614780/1‑151 (MQ=255)
                        gCGGTGCTCGGATATATCTATTCACTGCAAACCAGTATGTTGCTCATAACGTTGATTGGTTTCTTCCTGATTACTTTCGTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCCGTg                                                                                                                           <  6:1500528/151‑1 (MQ=255)
                           gTGCTCGGATATATCTATTCACTGCAAACCAGTATGTTGCTCATAACGTTGATTGGTTTCTTCCTGATTACTTTCGTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCCGTGGCt                                                                                                                        >  5:1471134/1‑151 (MQ=255)
                            tGCTCGGATATATCTATTCACTGCAAACCAGTATGTTGCTCATAACGTTGATTGGTTTCTTCCTGATTACTTTCGTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCCGTGGCTc                                                                                                                       >  3:484249/1‑151 (MQ=255)
                                cGGATATATCTATTCACTGCAAACCAGTATGTTGCTCATAACGTTGATTGGTTTCTTCCTGATTACTTTCGTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCCGTGGCTCCGGt                                                                                                                   <  1:612579/151‑1 (MQ=255)
                                 ggATATATCTATTCACTGCAAACCAGTATGTTGCTCATAACGTTGATTGGTTTCTTCCTGATTACTTTCGTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCCGTGGCTCCGGTc                                                                                                                  >  7:422074/1‑151 (MQ=255)
                                  gATATATCTATTCACTGCAAACCAGTATGTTGCTCATAACGTTGATTGGTTTCTTCCTGATTACTTTCGTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCCGTGGCTCCGGTCt                                                                                                                 <  7:1758054/151‑1 (MQ=255)
                                  gATATATCTATTCACTGCAAACCAGTATGTTGCTCATAACGTTGATTGGTTTCTTCCTGATTACTTTCGTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAAATCCGTGGCTCCGGTCt                                                                                                                 >  6:1046975/1‑151 (MQ=255)
                                   atatatCTATTCACTGCAAACCAGTATGTTGCTCATAACGTTGATTGGTTTCTTCCTGATTACTTTCGTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCATGAAAACTGGCCGACAGAGGCCAAACTCCGTGGCTCCGGTCTg                                                                                                                >  5:1961238/1‑151 (MQ=255)
                                    tatatCTATTCACTGCAAACCAGTATGTTGCTCATAACGTTGATTGGTTTCTTCCTGATTACTTTCGTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCCGTGGCTCCGGTCTgg                                                                                                               >  2:1823346/1‑151 (MQ=255)
                                     atatCTATTCACTGCAAACCAGTATGTTGCTCATAACGTTGATTGGTTTCTTCCTGATTACTTTCGTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCCGTGGCTCCGGTCTGGc                                                                                                              <  5:13108/151‑1 (MQ=255)
                                     atatCTATTCACTGCAAACCAGTATGTTGCTCATAACGTTGATTGGTTTCTTCCTGATTACTTTCGTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCCGTGGCTCCGGTCTGGc                                                                                                              >  8:285072/1‑151 (MQ=255)
                                      tatCTATTCACTGCAAACCAGTATGTTGCTCATAACGTTGATTGGTTTCTTCCTGATTACTTTCGTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCCGTGGCTCCGGTCTGGCg                                                                                                             >  1:1195697/1‑151 (MQ=255)
                                       atCTATTCACTGCAAACCAGTATGTTGCTCATAACGTTGATTGGTTTCTTCCTGATTACTTTCGTCTATATGTACGTTTGCTATGCCTCGGCAGTCTATGTCCCTGAAAACTGGCCGACAGAGCCCAAACTCCGTGGCTCCGGTCTGGCg                                                                                                             >  6:1818758/1‑150 (MQ=255)
                                        tCTATTCACTGCAAACCAGTATGTTGCTCATAACGTTGATTGGTTTCTTCCTGATTACTTTCGTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCCGTGGCTCCGGTCTGGCGa                                                                                                            >  7:102872/1‑150 (MQ=255)
                                        tCTATTCACTGCAAACCAGTATGTTGCTCATAACGTTGATTGGTTTCTTCCTGATTACTTTCGTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCCGTGGCTCCGGTCTGGCGa                                                                                                            >  3:913908/1‑150 (MQ=255)
                                           aTTCACTGCAAACCAGTATGTTGCTCATAACGTTGATTGGTTTCTTCCTGATTACTTTCGTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCCGTGGCTCCGGTCTGGCGAAt                                                                                                          <  1:27654/149‑1 (MQ=255)
                                           aTTCACTGCAAACCAGTATGTTGCTCATAACGTTGATTGGTTTCTTCCTGATTACTTTCGTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCCGTGGCTCCGGTCTGGCGAATGc                                                                                                        <  2:1425824/151‑1 (MQ=255)
                                           aTTCACTGCAAACCAGTATGTTGCTCATAACGTTGATTGGTTTCTTCCTGATTACTTTCGTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCCGTGGCTCCGGTCTGGCGAATGc                                                                                                        >  8:578596/1‑151 (MQ=255)
                                             tCACTGCAAACCAGTATGTTGCTCATAACGTTGATTGGTTTCTTCCTGATTACTTTCGTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCCGTGGCTCCGGTCTGGCGAATGCgg                                                                                                      >  7:1224361/1‑151 (MQ=255)
                                              cACTGCAAACCAGTATGTTGCTCATAACGTTGATTGGTTTCTTCCTGATTACTTTCGTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCCGTGGCTCCGGTCTGGCGAATGCGGt                                                                                                     >  3:852603/1‑151 (MQ=255)
                                              cACTGCAAACCAGTATGTTGCTCATAACGTTGATTGGTTTCTTCCTGATTACTTTCGTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCCGTGGCTCCGGTCTGGCGAATGCGGt                                                                                                     <  4:836009/151‑1 (MQ=255)
                                                cTGCAAACCAGTATGTTGCTCATAACGTTGATTGGTTTCTTCCTGATTACTTTCGTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCCGTGGCTCCGGTCTGGCGAATGCGGt                                                                                                     >  2:1129381/1‑149 (MQ=255)
                                                   cAAACCAGTATGTTGCTCATAACGTTGATTGGTTTCTTCCTGATTACTTTCGTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCCGTGGCTCCGGTCTGGCGAATGCGGTAGGGc                                                                                                >  5:793537/1‑151 (MQ=255)
                                                   cAAACCAGTATGTTGCTCATAACGTTGATTGGTTTCTTCCTGATTACTTTCGTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCCGTGGCTCCGGTCTGGCGAATGCGGTAGGGc                                                                                                >  2:906306/1‑151 (MQ=255)
                                                            aTGTTGCTCATAACGTTGATTGGTTTCTTCCTGATTACTTTCGTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCCGTGGCTCCGGTCTGGCGAATGCGGTAGGGCGAATCAGt                                                                                        <  5:697826/150‑1 (MQ=255)
                                                            aTGTTGCTCATAACGTTGATTGGCTTCTTCCTGATTACTTTCGTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCCGTGGCTCCGGTCTGGCGAATGCGGTAGGGCGAATCAGTg                                                                                       <  1:543507/151‑1 (MQ=255)
                                                             tGTTGCTCATAACGTTGATTGGTTTCTTCCTGATTACTTTCGTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCCGTGGCTCCGGTCTGGCGAATGCGGTAGGGCGAATCAGTgg                                                                                      >  8:1629541/1‑151 (MQ=255)
                                                                 gCTCATAACGTTGATTGGTTTCTTCCTGATTACTTTCGTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCCGTGGCTCCGGTCTGGCGAATGCGGTAGGGCGAATCAGTGGTAt                                                                                   <  1:1055918/150‑1 (MQ=255)
                                                                     aTAACGTTGATTGGTTGCTTCCTGATTACTTTCGTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCCGTGGCTCCGGTCTGGCGAATGCGGTAGGGCGAATCAGTGGTATTGCCg                                                                              <  4:31794/151‑1 (MQ=255)
                                                                       aaCGTTGATTGGTTTCTTCCTGATTACTTTCGTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCCGTGGCTCCGGTCTGGCGAATGCGGTAGGGCGAATCAGTGGTATTGCCga                                                                             >  3:1325458/1‑149 (MQ=255)
                                                                       aaCGTTGATTGGTTTCTTCCTGATTACTTTCGTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCCGTGGCTCCGGTCTGGCGAATGCGGTAGGGCGAATCAGTGGTATTGCAGc                                                                             >  4:120487/1‑150 (MQ=255)
                                                                             gATTGGTTTCTTCCTGATTACTTTCGTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCCGTGGCTCCGGTCTGGCGAATGCGGTAGGGCGAATCAGTGGTATTGCCGCACCTTAt                                                                      >  2:709753/1‑151 (MQ=255)
                                                                                 ggTTTCTTCCTGATTACTTTCGTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCCGTGGCTCCGGTCTGGCGAATGCGGTAGGGCGAATCAGTGGTATTGCCGCACCTTATGCCg                                                                  <  2:119487/151‑1 (MQ=255)
                                                                                 ggTTTCTTCCTGATTACTTTCGTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGACGACAGAGGCCAAACTCCGTGGCTCCTGTCTGGCGAATGCGGTAGGGCGAATCAGTGGTATTGCCGCACCTTATGCCg                                                                  <  4:1157268/151‑1 (MQ=255)
                                                                                 ggTTTCTTCCTGATTACTTTCGTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCAGAAATCTGGCCGACAGAGGCCAAACTCCGTGGCTACGGTCTGGCGAATGCGGTAGGGCGAATCAGTGCTATTTACGCACCTTATGc                                                                    >  7:359932/1‑149 (MQ=255)
                                                                                   tttCTTCCTGATTACTTTCGTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCCGTGGCTCCGGTCTGGCGAATGCGGTAGGGCGAATCAGTGGTATTGCCGCACCTTATGCCGtt                                                                >  2:1515254/1‑151 (MQ=255)
                                                                                    ttcttcCTGATTACTTTCGTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCCGTGGCTCCGGTCTGGCGAATGCGGTAGGGCGAATCAGTGGTATTGCCGCACCTTATGCCGTTg                                                               >  6:388671/1‑151 (MQ=255)
                                                                                      cttcCTGATTACTTTCGTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCCGTGGCTCCGGTCTGGCGAATGCGGTAGGGCGAATCAGTGGTATTGCCGCACCTTATGCCGTTGc                                                              >  3:650119/1‑150 (MQ=255)
                                                                                         ccTGATTACTTTCGTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCCGTGGCTCCGGTCTGGCGAATGCGGTAGGGCGAATCAGTGGTATTGCCGCACCTTATGCCGTTGCAGtg                                                          <  7:1831793/151‑1 (MQ=255)
                                                                                         ccTGATTACTTTCGTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCCGTGGCTCCGGTCTGGCGAATGCGGTAGGGCGAATCAGTGGTATTGCCGCACCTTATGCCGTTGCAGtg                                                          >  1:1384247/1‑151 (MQ=255)
                                                                                         ccTGATTACTTTCGTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCCGTGGCTCCGGTCTGGCGAATGCGGTAGGGCGAATCAGTGGTATTGCCGCACCTTATGCCGTTGCAGt                                                           <  5:1147637/150‑1 (MQ=255)
                                                                                          cTGATTACTTTCGTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCCGTGGCTCCGGTCTGGCGAATGCGGTAGGGCGAATCAGTGGTATTGCCGCACCTTATGCCGTTGCAGtgc                                                         >  3:1540086/1‑151 (MQ=255)
                                                                                           tGATTACTTTCGTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCCGTGGCTCCGGTCTGGCGAATGCGGTAGGGCGAATCAGTGGTATTGCCGCACCTTATGCCGTTGCAGtgct                                                        >  8:1029943/1‑151 (MQ=255)
                                                                                            gATTACTTTCGTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCCGTGGCTCCGGTCTGGCGAATGCGGTAGGGCGAATCAGTGGTATTGCCGCACCTTATGCCGTTGCAGtgctg                                                       <  7:218730/151‑1 (MQ=255)
                                                                                            gATTACTTTCGTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCCGTGGCTCCGGTCTGGCGAATGCGGTAGGGCGAATCAGTGGTATTGCCGCACCTTATGCCGTTGCAGtgct                                                        <  4:1342335/150‑1 (MQ=255)
                                                                                             aTTACTTTCGGCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCCGTGGCTCCGGTCTGGCGAATGCGGTAGGGCGAATCAGTGGTATTGCCGCACCTTATGCCGTTGCAGtgctgc                                                      <  4:971111/151‑1 (MQ=255)
                                                                                              ttACTTTCGTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCCGTGGCTCCGGTCTGGCGAATGCGGTAGGGCGAATCAGTGGTATTGCCGCACCTTATGCCGTTGCAGTgctgct                                                     >  6:473712/1‑151 (MQ=255)
                                                                                              ttACTTTCGTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCCGTGGCTCCGGTCTGGCGAATGCGGTAGGGCGAATCAGTGGTATTGCCGCACCTTATGCCGTTGCAGTgctgct                                                     >  2:335632/1‑151 (MQ=255)
                                                                                                   ttCGTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCCGTGGCTCCGGTCTGGCGAATGCGGTAGGGCGAATCAGTGGTATTGCCGCACCTTATGCCGTTGCAGTGCTGCTCagt                                                 >  2:1732899/1‑150 (MQ=255)
                                                                                                     cGTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCCGTGGCTCCGGTCTGGCGAATGCGGTAGGGCGAATCAGTGGTATTGCCGCACCTTATGCCGTTGCAGTGCTGCTCagtagt                                              >  7:1308464/1‑151 (MQ=255)
                                                                                                     cGTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCCGTGGCTCCGGTCTGGCGAATGCGGTAGGGCGAATCAGTGGTATTGCCGCACCTTATGCCGTTGCAGTGCTGCTCagtagt                                              >  1:843810/1‑151 (MQ=255)
                                                                                                     cGTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCCGTGGCTCCGGTCTGGCGAATGCGGTAGGGCGAATCAGTGGTATTGCCGCACCTTATGCCGTTGCAGTGCTGCTCagtagt                                              >  8:1494923/1‑151 (MQ=255)
                                                                                                      gTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCCGTGGCTCCGGTCTGGCGAATGCGGTAGGGCGAATCAGGGGTATTGCCGCACCTTATGCCGTTGCAGTGCTGCTCAGTAGtt                                             >  8:136542/1‑151 (MQ=255)
                                                                                                        cTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCCGTGGCTCCGGTCTGGCGAATGCGGTAGGGCGAATCAGTGGTATTGCCGCACCTTATGCCGTTGCAGTGCTGCTCAGTAGTTAt                                           >  3:16901/1‑151 (MQ=255)
                                                                                                        cTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCCGTGGCTCCGGTCTGGCGAATGCGGTAGGGCGAATCAGTGGTATTGCCGCACCTTATGCCGTTGCAGTGCTGCTCAGTAGTTAt                                           >  4:1004866/1‑151 (MQ=255)
                                                                                                         tataTGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCTGTGGCTCCGGTCTGGCGAATGCGGTAGGGCGAATCAGTGGTATTGCCGCACCTTATGCCGTTGCAGTGCTGCTCAGTAGTTATg                                          >  1:349234/1‑151 (MQ=255)
                                                                                                          atatGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCCGTGGCTCCGGTCTGGCGAATGCGGTAGGGCGAATCAGTGGTATTGCCGCACCTTATGCCGTTGCAGTGCTGCTCAGTAGTTTTgg                                         >  6:94557/1‑151 (MQ=255)
                                                                                                            atGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCCGTGGCTCCGGTCTGGCGAATGCGGTAGGGCGAATCAGTGGTATTGCCGCACCTTATGCCGTTGCAGTGCTGCTCAGTAGTTATGGc                                        <  8:815762/150‑1 (MQ=255)
                                                                                                            atGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCCGTGGCTCCGGTCTGGCGAATGCGGTAGGGCGAATCAGTGGTATTGCCGCACCTTATGCCGTTGCAGTGCTGCTCAGTAGTTATGGCg                                       >  3:1895022/1‑151 (MQ=255)
                                                                                                             tGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCCGTGGCTCCGGTCTGGCGAATGCGGTAGGGCGAATCAGTGGTATTGCCGCACCTTATGCCGTTGCAGTGCTGCTCAGTAGTTATGGCGt                                      >  8:1773369/1‑151 (MQ=255)
                                                                                                              gTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCCGTGGCTCCGGTCTGGCGAATGCGGTAGGGCGAATCAGTGGTATTGCCGCACCTTATGCCGTTGCAGTGCTGCTCAGTAGTTATGGCGtt                                     >  5:1252773/1‑151 (MQ=255)
                                                                                                                aCGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCCGTGGCTCCGGTCTGGCGAATGCGGTAGGGCGAATCAGTGGTATTGCCGCACCTTATGCCGTTGCAGTGCTGCTCAGTAGTTATGGCGTTa                                    <  5:1785377/150‑1 (MQ=255)
                                                                                                                aCGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCCGTGGCTCCGGTCTGGCGAATGCGGTAGGGCGAATCAGTGGTATTGCCGCACCTTATGCCGTTGCAGTGCTGCTCAGTAGTTATGGCGTTa                                    <  7:519050/150‑1 (MQ=255)
                                                                                                                aCGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCCGTGGCTCCGGTCTGGCGAATGCGGTAGGGCGAATCAGTGGTATTGCCGCACCTTATGCCGTTGCAGTGCTGCTCAGTAGTTATGGCGTTAc                                   <  1:1579954/151‑1 (MQ=255)
                                                                                                                 cGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCCGTGGCTCCGGTCTGGCGAATGCGGTAGGGCGAATCAGTGGTATTGCCGCACCTTATGCCGTTGCAGTGCTGCTCAGTAGTTATGGCGTTACg                                  <  1:43301/151‑1 (MQ=255)
                                                                                                                  gTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCCGTGGCTCCGGTCTGGCGAATGCGGTAGGGCGAATCAGTGGTATTGCCGCACCTTATGCCGTTGCAGTGCTGCTCAGTAGTTATGGCGTTACgg                                 >  2:203368/1‑151 (MQ=255)
                                                                                                                      gCTATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCCGTGGCTCCGGTCTGGCGAATGCGGTAGGGCGAATCAGTGGTATTGCCGCACCTTATGCCGTTGCAGTGCTGCTCAGTAGTTATGGCGTTACGGGAg                              <  8:1218671/150‑1 (MQ=255)
                                                                                                                        tATGCCTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCCGTGGCTCCGGTCTGGCGAATGCGGTAGGGCGAATCAGTGGTATTGCCGCACCTTATGCCGTTGCAGTGCTGCTCAGTAGTTATGGCGTTACGGGAGTCt                           >  5:1675326/1‑151 (MQ=255)
                                                                                                                            ccTCGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCCGTGGCTCCGGTCTGGCGAATGCGGTAGGGCGAATCAGTGGTATTGCCGCACCTTATGCCGTTGCAGTGCTGCTCAGTAGTTATGGCGTTACGGGAGTCTTTAt                       >  7:1149553/1‑151 (MQ=255)
                                                                                                                               cGGCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCCGTGGCTCCGGTCTGGCGAATGCGGTAGGGCGAATCAGTGGTATTGCCGCACCTTATGCCGTTGCAGTGCTGCTCAGTAGTTATGGCGTTACGGGAGTCTTTAttct                    <  4:1893848/151‑1 (MQ=255)
                                                                                                                                 gCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCCGTGGCTCCGGTCTGGCGAATGCGGTAGGGCGAATCAGTGGTATTGCCGCACCTTATGCCGTTGCAGTGCTGCTCAGTAGTTATGGCGTTACGGGAGTCTTTAttcttc                  >  3:1102017/1‑151 (MQ=255)
                                                                                                                                 gCAGTGTATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCCGTGGCTCCGGTCTGGCGAATGCGGTAGGGCGAATCAGTGGTATTGCCGCACCTTATGCCGTTGCAGTGCTGCTCAGTAGTTATGGCGTTACGGGAGTCTTTAttcttc                  <  5:867940/151‑1 (MQ=255)
                                                                                                                                    gtgtATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCCGTGGCTCCGGTCTGGCGAATGCGGTAGGGCGAATCAGTGGTATTGCCGCACCTTATGCCGTTGCAGTGCTGCTCAGTAGTTATGGCGTTACGGGAGTCTTTATTCTTCTgg               >  3:1081473/1‑151 (MQ=255)
                                                                                                                                       tATGTCCCTGAAAACTGGCCGACAGAGGCCAAACTCCGTGGCTCCGGTCTGGCGAATGCGGTAGGGCGAATCAGTGGTATTGCCGCACCTTATGCCGTTGCAGTGCTGCTCAGTAGTTATGGCGTTACGGGAGTCTTTATTCTTCTggggg            >  4:74490/1‑151 (MQ=255)
                                                                                                                                           tCCCTGAAAACTGGCCGACAGAGGCCAAACTCCGTGGCTCCGGTCTGGCGAATGCGGTAGGGCGAATCAGTGGTATTGCCGCACCTTATGCCGTTGCAGTGCTGCTCAGTAGTTATGGCGTTACGGGAGTCTTTATTCTTCTGGGGGCGGt        >  7:925781/1‑151 (MQ=255)
                                                                                                                                              cTGAAAACTGGCCGACAGAGGCCAAACTCCGTGGCTCCGGTCTGGCGAATGCGGTAGGGCGAATCAGTGGTATTGCCGCACCTTATGCCGTTGCAGTGCTGCTCAGTAGTTATGGCGTTACGGGAGTCTTTATTCTTCTGGGGGCGGTTTc     >  3:1442718/1‑151 (MQ=255)
                                                                                                                                               tGAAAACTGGCCGACAGAGGCCAAACTCCGTGGCTCCGGTCTGGCGAATGCGGTAGGGCGAATCAGTGGTATTGCCGCACCTTATGCCGTTGCAGTGCTGCTCAGTAGTTATGGCGTTACGGGAGTCTTTATTCTTCTGGGGGCGGTTTc     >  8:1124642/1‑150 (MQ=255)
                                                                                                                                                taaaaCTGGCCTACAGAGGCAAAACTCCGTGGCTCCGGTCTGGCGAATGCGGTAGGGCGAATCAGTGGTATTGCCGCACCTTATGACGTTGCAGTGCTGATCAGTAGTTATGGCGTTACGGGAGTCTTTATTCTTCTGGGGGCGGTTTCa    <  4:1663655/149‑1 (MQ=255)
                                                                                                                                                 aaaaCTGGCCGACAGAGGCCAAACTCCGTGGCTCCGGTCTGGCGAATGCGGTAGGCCGAATCAGTGGTATTGCCGCACCTTATGCAGTTGCAGTGCTGCTCAGTAGTTATGGCGTTACGGCAGTCTTTATTCTTCAGGGGGCGGTTTCAat  >  8:425759/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GGTGTGGGGCTATTAATCCTGATTGCGGTGCTCGGATATATCTATTCACTGCAAACCAGTATGTTGCTCATAACGTTGATTGGTTTCTTCCTGATTACTTTCGTCTATATGTACGTTTGCTATGCCTCGGCAGTGTATGTCCCTGAAATCTGGCCGACAGAGGCCAAACTCCGTGGCTCCGGTCTGGCGAATGCGGTAGGGCGAATCAGTGGTATTGCCGCACCTTATGCCGTTGCAGTGCTGCTCAGTAGTTATGGCGTTACGGGAGTCTTTATTCTTCTGGGGGCGGTTTCAAT  >  DH1/2019242‑2019537

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑