Predicted mutation
evidence seq id position mutation annotation gene description
RA DH1 2,244,937 A→G intergenic (‑81/+236) pinQ ← / ← ydfK Qin prophage; site‑specific recombinase/cold‑shock protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH12,244,9370AG98.0% 286.5 / ‑2.4 148intergenic (‑81/+236)pinQ/ydfKQin prophage; site‑specific recombinase/cold‑shock protein
Reads supporting (aligned to +/- strand):  ref base A (1/2);  new base G (67/78);  total (68/80)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 155 total aligned reads displayed.

TCCCGGCGTTGATTTTCGGTGGTCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTATGTACACGTTCGAAAATAAACGAATGCGTATGCAACCCCGTAATTTTGGTGAGACCCAAAAT  >  DH1/2244789‑2244999
                                                                                                                                                    |                                                              
tCCCGGCGTTGATTTTCGGTGGTCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTtgtat                                                               <  2:1736575/150‑1 (MQ=31)
 gccGGGGTTGATTTTCGGTGGTCTGATCGAGCGTTGATATCCGACAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTtgtgtgt                                                             <  8:1116267/150‑1 (MQ=9)
 cgcgtagTTGATTTTCGTTTGTCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTTGAGACATAGTGAGAGTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTtgtgtg                                                              <  8:3067/144‑1 (MQ=11)
 cgcGGCGTTGATTTTCGGTGGTCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTtgtgtgt                                                             <  1:423014/149‑1 (MQ=14)
  ccGGCGTTGATTTTCGGTGGTCTGATCAAGCGTTGATATCCGACAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTtgtgtgt                                                             >  4:43221/1‑150 (MQ=14)
   cgtagTTGATTTTCGGTGGTCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTac                                                           <  2:1297098/147‑1 (MQ=14)
   cGGCGTTGATTTTCGGTGGTCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTac                                                           <  6:932942/151‑1 (MQ=14)
   cGGCGTTGATTTTCGGTGGTCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTac                                                           >  2:1234218/1‑151 (MQ=14)
    ggtgTTGATTTTTTGTGGTCTGATTGTGGGTTGATTTCCGATAGTTAGCAAATATTTGAGAGATAGTGAGTGTCTTTTCGTTATTTGTGTTCATTTCATTATGGATGTCAGAAATTAATTATGATTATTTTTGTGCATATTTGTATTTaca                                                          <  6:289315/148‑1 (MQ=2)
    ggCGTTGATTTTCGGTGGTCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTaca                                                          <  4:1487721/151‑1 (MQ=14)
     gCGTTGATTTTCGGTGGTCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTacac                                                         <  1:1501534/151‑1 (MQ=14)
     gCGTTGATTTTCGGTGGTCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTacac                                                         <  1:1492436/151‑1 (MQ=14)
     gCGTTGATTTTCGGTGGTCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTacac                                                         >  8:675906/1‑151 (MQ=14)
      cGTTGATTTTCGGTGGTCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACg                                                        >  1:1233559/1‑151 (MQ=14)
       gTTGATTTTCGGTGGTCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGt                                                       >  3:58525/1‑151 (MQ=14)
         tGATTTTCGGTGGTCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTc                                                     >  7:35659/1‑151 (MQ=14)
          gATTTTCGGTGGTCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTc                                                     <  8:1282485/150‑1 (MQ=14)
           aTTTTCGGTGGTCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGa                                                   <  7:1044309/151‑1 (MQ=14)
            ttttCGGTGGTCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGa                                                   >  5:1389328/1‑150 (MQ=14)
             tttCGGTGGTCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGaa                                                  <  8:1840759/150‑1 (MQ=14)
              ttCGGTGGTGTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGaaa                                                 <  6:933274/150‑1 (MQ=14)
              ttCGGTGGTCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGaaa                                                 >  6:1658260/1‑150 (MQ=14)
               tCGGTGGTCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGaaaa                                                <  6:490589/150‑1 (MQ=14)
                cGGTGGTCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGAAAATa                                              <  8:471982/151‑1 (MQ=14)
                 ggtggtCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGAAAATaa                                             <  1:1096536/151‑1 (MQ=14)
                  gtggtCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGAAAATaaa                                            <  8:1516584/151‑1 (MQ=14)
                   tggtCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGAAAATAAAc                                           >  2:1065771/1‑151 (MQ=14)
                   tggtCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGAAAATAAAc                                           >  4:1805173/1‑151 (MQ=14)
                   tggtCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACAAGTTCGAAAATAAAc                                           >  2:1565596/1‑151 (MQ=14)
                    ggtCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGAAAGTAAAc                                           <  3:1868307/150‑1 (MQ=14)
                    ggtCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGAAAATAAAc                                           <  6:1249879/150‑1 (MQ=14)
                    agtCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGAAAATAAACg                                          >  4:1229737/2‑151 (MQ=14)
                      tCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGAAAATAAACGa                                         >  1:661063/1‑150 (MQ=14)
                         gATCCATCGTTGATATCCGACAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGAAAATAAACGAATg                                      <  8:805752/150‑1 (MQ=14)
                         gATCCAGCGTTGATATCCGACAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGAAAATAAACGaa                                        <  3:1705635/148‑1 (MQ=14)
                         gATCCAGCGTTGATATCCGACAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGAAAATAAACGAAt                                       <  5:1403290/149‑1 (MQ=14)
                         gATCCAGCGTTGATATCCGACAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGAAAATAAACGAATg                                      <  8:1798638/150‑1 (MQ=14)
                         gATCCAGCGTTGATATCCGACAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGAAAATAAACGAATGc                                     >  7:352151/1‑151 (MQ=14)
                         gATCCAGCGTTGATATCCGACAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGAAAATAAACGAATGc                                     >  5:740704/1‑151 (MQ=14)
                         gATCCAGCATTGATATCCGACAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGAAAATAAACGAAt                                       <  1:251013/149‑1 (MQ=14)
                          aTCCAGCGTTGATATCCGACAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGAAAATAAACGAATGCg                                    >  5:1692860/1‑151 (MQ=14)
                          aTCCAGCGTTGATATCCGACAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGAAAATAAACGAATGCg                                    >  8:291978/1‑151 (MQ=14)
                            ccAGCGTTGATATCCGACAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGAAAATAAACGAATGCGt                                   <  1:1230428/150‑1 (MQ=14)
                            ccAGCGTTGATATCCGACAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGAAAATAAACGAATGCGt                                   <  3:1878513/150‑1 (MQ=14)
                            ccAGCGTTGATATCCGACAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGAACACGTTCGAAAATAAACGAATGCGt                                   <  2:1793868/150‑1 (MQ=14)
                             cAGCGTTGATATCCGACAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGAAAATAAACGAATGCGTAt                                 <  5:1829269/151‑1 (MQ=14)
                             cAGCGTTGATATCCGACAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGAAAATAAACGAATGCGTAt                                 <  4:1386029/151‑1 (MQ=14)
                              aGCGTTGATATCCGACAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGAAAATAAACGAATGCGTATg                                >  3:1489840/1‑151 (MQ=14)
                              aGCGTTGATATCCGACAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGAAAATAAACGAATGCGTATg                                <  3:1200175/151‑1 (MQ=14)
                               gCGGTGATATCCGACAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGAAAATAAACGAATGCGTATGc                               <  8:1529715/151‑1 (MQ=14)
                                cGTTGATATCCGACAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGAAAATAAACGAATGCGTATGCa                              <  5:240336/151‑1 (MQ=14)
                                cGTTGATATCCGACAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGAAAATAAACGAATGCGTATGCa                              <  4:106495/151‑1 (MQ=14)
                                 gTTGATATCCGACAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGAAAATAAACGAATGCGTATGCa                              >  4:692393/1‑150 (MQ=14)
                                 gTTGATATCCGACAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGAAAATAAACGAATGCGTATGCa                              >  8:1506561/1‑150 (MQ=14)
                                  ttGATATCCGACAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGAAAATAAACGAATGCGTATGCAAc                            >  4:1139095/1‑151 (MQ=14)
                                    gATATCCGACAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGAAAATAAACGAATGCGTATGCAAcc                           <  5:1753993/150‑1 (MQ=14)
                                     atatGCGACAGAAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGAAAATAAACGAATGCGTATGCAAcccc                         >  7:233082/1‑151 (MQ=9)
                                      tatCCGACAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGAAAATAAACGAATGCGTATGCAACCCCg                        >  8:1339116/1‑151 (MQ=14)
                                      tatCCGACAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGAAAATAAACGAATGCGTATGCAACCCCg                        >  7:543960/1‑151 (MQ=14)
                                       atCCGACAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGAAAATAAACGAATGCGTATGCAACCCCg                        <  3:144668/150‑1 (MQ=14)
                                       atCCGACAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGAAAATAAACGAATGCGTATGCAACCCCGt                       >  4:295173/1‑151 (MQ=14)
                                       atCCGACAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGAAAATAAACGAATGCGTATGCAACCCCGt                       >  3:591101/1‑151 (MQ=14)
                                       atCCGACAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGAAAATAAACGAATGCGTATGCAACCCCGt                       <  7:1837480/151‑1 (MQ=14)
                                       atCCGACAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGAAAATAAACGAATGCGTATGCAACCCCGt                       >  4:1884935/1‑151 (MQ=14)
                                       atCCGACAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGAAAATAAACGAATGCGTATGCAACCCCGt                       >  4:912413/1‑151 (MQ=14)
                                       atCCGACAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGAAAATAAACGAATGCGTATGCAACCACGt                       >  2:389063/1‑151 (MQ=14)
                                        tCCGACAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGAAAATAAACGAATGCGTATGCAACCCCGt                       >  2:1832487/1‑150 (MQ=14)
                                         ccGACAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGAAAATAAACGAATGCGTATGCAACCCCGTa                      <  7:997492/150‑1 (MQ=14)
                                          cGACAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGAAAATAAACGAATGCGTATGCAACCCCGTAAt                    <  3:1528946/151‑1 (MQ=14)
                                           gACAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGAAAATAAACGAATGCGTATGCAACCCCGTAAtt                   <  8:1526582/151‑1 (MQ=14)
                                           gACAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGAAAATAAACGAATGCGTATGCAACCCCGTAAtt                   >  3:1611464/1‑151 (MQ=14)
                                           gACAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGAAAATAAACGAATGCGTATGCAACCCCGTAAtt                   >  4:1620369/1‑151 (MQ=14)
                                           gACAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGAAAATAAACGAATGCGTATGCAACCCCGTAAtt                   >  6:1192699/1‑151 (MQ=14)
                                           gACAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGAAAATAAACGAATGCGTATGCAACCCCGTAAtt                   >  8:661165/1‑151 (MQ=14)
                                            aCAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGAAAATAAACGAATGCGTATGCAACCCCGTAAttt                  <  7:1716941/151‑1 (MQ=14)
                                              aGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGAAAATAAACGAATGCGTATGCAACCCCGTAAttttt                >  8:486165/1‑150 (MQ=14)
                                              aGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGAAAATAAACGAATGCGTATGCAACCCCGTAATTTTg                <  1:877676/151‑1 (MQ=14)
                                              aGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGAAAATAAACGAATGCGTATGCAACCCCGTAATTTTg                >  5:859831/1‑151 (MQ=14)
                                                tAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGAAAATAAACGAATGCGTATGCAACCCCGTAATTTTGGt              >  2:92705/1‑151 (MQ=14)
                                                 aaGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGAAAATAAACGAATGCGTATGCAACCCCGTAATTTTGGTg             <  6:1858184/151‑1 (MQ=14)
                                                 aaGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGAAAATAAACGAATGCGTATGCAACCCCGTAATTTTGGTg             >  6:1721951/1‑151 (MQ=14)
                                                 aaGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGAAAATAAACGAATGCGTATGCAACCCCGTAATTTTGGTg             <  5:963757/151‑1 (MQ=14)
                                                 aaGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTCCGAAAATAAACGAATGCGTATGCAACCCCGTAATTTTGGTg             >  4:410666/1‑151 (MQ=14)
                                                  aGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGAAAATAAACGAATGCGTATGCAACCCCGTAATTTTGGTga            <  7:1241267/151‑1 (MQ=14)
                                                   gCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGAAAATAAACGAATGCGTATGCAACCCCGTAATTTTGGTgag           <  6:496662/151‑1 (MQ=14)
                                                   gCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGAAAATAAACGAATGCGTATGCAACCCCGTAATTTTGGTgag           <  2:432000/151‑1 (MQ=14)
                                                    cAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGAAAATAAACGAATGCGTATGCAACCCCGTAATTTTGGTg             >  7:926132/1‑148 (MQ=14)
                                                    cAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGAAAATAAACGAATGCGTATGCAACCCCGTAATTTTGGTg             >  8:373347/1‑148 (MQ=14)
                                                     aaaaaTTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGAAAATAAACGAATGCGTATGCAACCCCGTAATTTTGGTGAGAc         >  8:1974792/1‑151 (MQ=14)
                                                      aaaaTTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGAAAATAAACGAATGCGTATGCAACCCCGTAATTTTGGTGAGAcc        <  3:1519263/151‑1 (MQ=14)
                                                      aaaaTTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGAAAATAAACGAATGCGTATGCAACCCCGTAATTTTGGTGAGAcc        <  1:1051967/151‑1 (MQ=14)
                                                       aaaTTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGAAAATAAACGAATGCGTATGCAACCCCGTAATTTTGGTGAGAccc       >  8:601640/1‑151 (MQ=14)
                                                         aTTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGAAAATAAACGAATGCGTATGCAACCCCGTAATTTTGGTGAGACCCa      >  4:1567623/1‑150 (MQ=14)
                                                         aTTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTATGTACACGTTCGAAAATAAACGAATGCGTATGCAACCCCGTAATTTTGGTGAGACCCaa     <  3:413682/151‑1 (MQ=31)
                                                          tttGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGAAAATAAACGAATGCGTATGCAACCCCGTAATTTTGGTGAGACCCaa     >  3:1223281/1‑150 (MQ=14)
                                                           ttGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGAAAATAAACGAATGAGTATGCAAACCCGTAATTTTGGATAGACCCaaa    >  4:228923/1‑150 (MQ=255)
                                                            tGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGAAAATAAACGAATGCGTATGCAACCCCGTAATTTTGGTGAGACCCaaaa   <  5:1076666/150‑1 (MQ=11)
                                                            tGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGAAAATAAACGAATGCGTATGCAACCCCGTAATTTTGGTGAGACCCAAAAt  >  7:529993/1‑151 (MQ=11)
                                                            tGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGAAAATAAACGAATGCGTATGCAACCCCGTAATTTTGGTGAGACCCAAAAt  >  3:999814/1‑151 (MQ=11)
                                                            tGAGACATAGTGAGAATCTATACGTAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTGTGTACACGTTCGAAAATAAACGAATGCGTATGCAACCCCGTAATTTTGGTGAGACCCaaaa   <  4:1172936/150‑1 (MQ=2)
                                                                                                                                                    |                                                              
TCCCGGCGTTGATTTTCGGTGGTCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTTGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTATGTACACGTTCGAAAATAAACGAATGCGTATGCAACCCCGTAATTTTGGTGAGACCCAAAAT  >  DH1/2244789‑2244999

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: