Predicted mutation
evidence seq id position mutation annotation gene description
RA DH1 3,297,355 A→T N28K (AAT→AAA)  appY ← transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH13,297,3550AT97.7% 2230.5 / 22.3 603N28K (AAT→AAA) appYtranscriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (7/7);  new base T (312/277);  total (319/284)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.95e-01

Only 100 of 622 total aligned reads displayed.

TCTTATCAAGAGTAAATTTGCCAGGCAGCTCATTATTCACGTCGATAAAAAAGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGA‑ATTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATCCATCTTGCACCTATCATAAAATTAAAACAAGTTGATAGTAGTCAAATAACAACCAATTAAATACAC  >  DH1/3297207‑3297501
                                                                                                                                                     |                                                                                                                                                  
tCTTATCAAGAGTAAATTTGCCAGGCAGCTCATTATTCACGTCGATAAAAAAGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGA‑ttt                                                                                                                                                  >  3:1554252/1‑151 (MQ=255)
 cTTATCAAGAGTAAATTTGCCAGGCAGCTCATTATTCACGTCGATAAAAAAGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGA‑tttt                                                                                                                                                 >  5:1447688/1‑151 (MQ=255)
  ttATCAAGAGTAAATTTGCCAGGCAGCTCATTATTCACGTCGATAAAAAAGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGA‑Ttttt                                                                                                                                                >  5:1279667/1‑151 (MQ=255)
   tATCAAGAGTAAATTTGCCAGGCAGCTCATTATTCACGTCGATAAAAAAGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGA‑tttt                                                                                                                                                 <  2:1952230/149‑1 (MQ=255)
   tATCAAGAGTAAATTTGCCAGGCAGCTCATTATTCACGTCGATAAAAAAGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGA‑tttt                                                                                                                                                 <  1:894802/149‑1 (MQ=255)
    aTCAAGAGTAAATTTGCCAGGCAGCTCATTATTCACGTCGATAAAAAAGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGA‑Ttttttt                                                                                                                                              <  6:1271520/151‑1 (MQ=255)
      cAAGAGTAAATTTGCCAGGCAGCTCATTATTCACGTCGATAAAAAAGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGA‑tttttttt                                                                                                                                             <  2:1599918/150‑1 (MQ=255)
      cAAGAGTAAATTTGCCAGGCAGCTCATTATTCACGTCGATAAAAAAGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGA‑Ttttttttt                                                                                                                                            >  1:1237584/1‑151 (MQ=255)
       aaGAGTAAATTTGCCAGGCAGCTCATTATTCACGTCGATAAAAAAGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGA‑TTTTTTTTTg                                                                                                                                           <  7:957361/151‑1 (MQ=255)
        agagTAAATTTGCCCGGCAGCTCATTATTCACGTCGATAAAAAAGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGA‑TTTTTTTTTGa                                                                                                                                          <  1:505422/151‑1 (MQ=255)
         gagTAAATTTGCCAGGCAGCTCATTATTCACGTCGATAAAAAAGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGA‑TTTTTTTTTGaa                                                                                                                                         <  5:1977155/151‑1 (MQ=255)
           gTAAATTTGCCAGGCAGCTCATTATTCACGTCGATAAAAAAGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATG‑‑ATTTTTTTTGAACga                                                                                                                                      <  8:1533830/151‑1 (MQ=255)
             aaaTTTGCCAGGCAGCTCATTATTCACGTCGATAAAAAAGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGA‑TTTTTTTTTGAACga                                                                                                                                      <  8:1923578/150‑1 (MQ=255)
               aTTTGCCAGGCAGCTCATTATTCACGTCGATAAAAAAGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAATAATGA‑TTTTTTTTTGAACg                                                                                                                                       >  7:1078337/1‑147 (MQ=255)
               aTTTGCCAGGCAGCTCATTATTCACGTCGATAAAAAAGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGA‑TTTTTTTTTGAACGAGAt                                                                                                                                   <  6:1074282/151‑1 (MQ=255)
                 ttGCCAGGCAGCTCATTATTCACGTCGATAAAAAAGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGA‑TTTTTTTTTGAACGAGATa                                                                                                                                  <  7:1008604/150‑1 (MQ=255)
                   gCCAGGCAGCTCATTATTCACGTCGATAAAAAAGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGA‑TTTTTTTTTGAACGAGATAAc                                                                                                                                <  3:539584/150‑1 (MQ=255)
                    ccAGGCAGCTCATTATTCACGTCGATAAAAAAGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGA‑TTTTTTTTTGAACGAGATAActc                                                                                                                              <  4:851468/151‑1 (MQ=255)
                     cAGGCAGCTCATTATTCACGTCGATAAAAAAGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGA‑TTTTTTTTTGAACGAGATAActct                                                                                                                             <  1:589419/151‑1 (MQ=255)
                         cAGCTCATTATTCAGGTCGATAAAAAAGGATAAACATGTTTTCTTATCTATATCAAAAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTTTCGCTTACAATAAACAATGA‑TTTTTTTTTGAACGAGATAACTCTCCTg                                                                                                                         <  2:973359/151‑1 (MQ=255)
                          aGCTCATTATTCACGTCGATAAAAAAGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGCGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGA‑TTTTTTTTTGAACGAGATAACTCTCCTGt                                                                                                                        <  4:620665/151‑1 (MQ=255)
                             tCATTATTCACGTCGATAAAAAAGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGA‑TTTTTTTTTGAACGAGATAACTCTCCTGttt                                                                                                                      >  7:457692/1‑150 (MQ=255)
                             tCATTATTCACGTCGATAAAAAAGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTACACGGGCATACTGGTAACTCCCTACTAAATTTGACGCTTACAATAAACAATGA‑TTATTTTATGAACGAGATAACTCTCCtcatt                                                                                                                      >  2:355182/1‑146 (MQ=255)
                              cATTAAACACGTCGATAAAAAAGGATAAACAAGTTTTCTAATCTATATCAACAATTCTTAGTTTAGACCGGCATACTGGCAACTCCCTTCAAATTTTGTCGCTTACAATAAACAATGA‑TTTTTTTTTGAACGAGATAACTCTCCTGttt                                                                                                                      <  4:296326/149‑1 (MQ=255)
                                 tattCACGTCGATAAAAAAGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGA‑TTTTTTTTTGAACGAGATAACTCTCCTGTTTATaa                                                                                                                  <  1:1750846/150‑1 (MQ=255)
                                 tattCACGTCGATAAAAAAGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGA‑TTTTTTTTTGAACGAGATAACTCTCCTGTTTATaa                                                                                                                  <  7:549139/150‑1 (MQ=255)
                                  attCACGTCGATAAAAAAGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGA‑TTTTTTTTTGAACGAGATAACTCTCCTGTTTATAAt                                                                                                                 <  4:9226/150‑1 (MQ=255)
                                    tCACGTCGATAAAAAAGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGA‑TTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTa                                                                                                               >  2:454086/1‑150 (MQ=255)
                                     cACGTCGATAGAAAAGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGA‑TTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTaa                                                                                                              <  8:351180/150‑1 (MQ=255)
                                      aCGTCGATAAAAAAGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGA‑TTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAAt                                                                                                            >  2:1389662/1‑151 (MQ=255)
                                      aCGTCGATAAAAAAGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGA‑TTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAAt                                                                                                            <  5:1800331/151‑1 (MQ=255)
                                            aTAAAAAAGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGA‑TTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAAtt                                                                                                      >  2:253744/1‑150 (MQ=255)
                                            aTAAAAAAGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGA‑TTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATg                                                                                                      <  5:430487/151‑1 (MQ=255)
                                             tAAAAAAGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGA‑TTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATg                                                                                                      >  2:1225264/1‑150 (MQ=255)
                                              aaaaaaGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGA‑TTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGAt                                                                                                    <  1:1550251/151‑1 (MQ=255)
                                              aaaaaaGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGA‑TTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGAt                                                                                                    <  7:671783/151‑1 (MQ=255)
                                              aaaaaaGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGA‑TTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGAt                                                                                                    >  3:1660138/1‑151 (MQ=255)
                                               aaaaaGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCAAATTTTGTCGCTTACAATAAACAATGA‑TTTTTTTTTTAACGAGATAACTATCCTGTTTATAATTAAATCAAATGAtt                                                                                                   >  6:458514/1‑151 (MQ=255)
                                                aaaaGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGA‑TTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTg                                                                                                  <  4:765224/151‑1 (MQ=255)
                                                 aaaTGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGA‑TTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGa                                                                                                 <  6:783475/151‑1 (MQ=255)
                                                 aaaGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGA‑TTTTTTTTTGAACGAGATAACTATCCTGTTTATAATTAAATAAAATTATTg                                                                                                  >  1:1647998/1‑150 (MQ=255)
                                                   aGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGA‑TTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGAc                                                                                                >  8:1078341/1‑150 (MQ=255)
                                                          aCATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGA‑TTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGaaa                                                                                        <  1:1066617/151‑1 (MQ=255)
                                                           cATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGA‑TTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGaaa                                                                                        >  4:485201/1‑150 (MQ=255)
                                                               ttttCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGA‑TTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTAc                                                                                   >  5:187499/1‑151 (MQ=255)
                                                                tttCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGA‑TTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGagatcggaagagcaac                                                                                  >  7:993054/1‑136 (MQ=255)
                                                                tttCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGA‑TTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTAc                                                                                   <  7:327938/150‑1 (MQ=255)
                                                                tttCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGA‑TTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACg                                                                                  >  6:631700/1‑151 (MQ=255)
                                                                tttCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGA‑TTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACg                                                                                  >  6:1879745/1‑151 (MQ=255)
                                                                  tCTTATGTATATGAACAATTCTTACTTTAGAGGGGCAAACTTGTAACTCCCTTCTAATTTTTTCGCTTACAATAAACAATGA‑TTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACgg                                                                                 <  1:1444332/150‑1 (MQ=255)
                                                                  tCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGA‑TTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACgg                                                                                 >  3:126487/1‑150 (MQ=255)
                                                                    ttATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGA‑TTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGc                                                                              >  6:1461859/1‑151 (MQ=255)
                                                                     tATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGA‑TTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGc                                                                              >  4:1396606/1‑150 (MQ=255)
                                                                     tATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTCACAATAAACAATGA‑TTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGc                                                                              >  7:73516/1‑150 (MQ=255)
                                                                      aTCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGA‑TTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCa                                                                             >  1:1337412/1‑150 (MQ=255)
                                                                      aTCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGA‑TTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCa                                                                             >  5:73399/1‑150 (MQ=255)
                                                                       tCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGA‑TTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCaa                                                                            >  8:1280340/1‑150 (MQ=255)
                                                                        cTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGA‑TTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAAc                                                                          >  2:1769642/1‑151 (MQ=255)
                                                                          atatCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGA‑TTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACAt                                                                        >  7:1841145/1‑151 (MQ=255)
                                                                          atatCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGA‑TTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATCAAAACTACGGAGCAAACAt                                                                        <  1:220897/151‑1 (MQ=255)
                                                                           tatCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGA‑TTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACAt                                                                        >  7:1439045/1‑150 (MQ=255)
                                                                            atCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGA‑TTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATa                                                                       >  2:770431/1‑150 (MQ=255)
                                                                             tCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGA‑TTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTAc                                                                                   <  8:137818/137‑1 (MQ=255)
                                                                                acaaTTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATG‑‑TTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATCCAt                                                                 <  1:418500/151‑1 (MQ=255)
                                                                                 caaTTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGA‑TTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATCCa                                                                  >  4:1947061/1‑150 (MQ=255)
                                                                                   aTTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGA‑TTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATCCATCt                                                               >  1:1002746/1‑151 (MQ=255)
                                                                                     tCTTATTTTATAGGGGCATACTTGTAACTCCCTTCTAATTTTTTCGCTTACAATAAACAATTT‑TTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATCCATCtt                                                              <  6:892094/150‑1 (MQ=255)
                                                                                     tCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGATTTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATCCATCtt                                                              >  5:1559902/1‑151 (MQ=255)
                                                                                     tCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGA‑TTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATCCATCtt                                                              <  5:1120182/150‑1 (MQ=255)
                                                                                     tCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGA‑ATTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGTTGAAAACTACGGAGCAAACATAATCCATCtt                                                              <  4:159657/150‑1 (MQ=255)
                                                                                      cTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGA‑TTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATCCATCTTGc                                                            >  5:256038/1‑151 (MQ=255)
                                                                                       ttAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGA‑TTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATCCATCTTGc                                                            >  2:1883388/1‑150 (MQ=255)
                                                                                          gTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGA‑TTTTTTTTTGAACGAGATGACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAGACTACGGAGCAAACATAATCCATCTTGCACCt                                                        <  4:1061360/151‑1 (MQ=255)
                                                                                           tttAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGA‑TTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATCCATCTTGCACCt                                                        >  3:868862/1‑150 (MQ=255)
                                                                                            ttAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGA‑TTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATCCATCTTGCACCTa                                                       <  6:1635503/150‑1 (MQ=255)
                                                                                             tAGAGGTGCATACTGGTAACTCCCTTCTAATTTTGTAGCTTACAATAAACAATGA‑TTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATCCATCTTGCACCTAt                                                      <  1:1912902/150‑1 (MQ=255)
                                                                                                agGGGCATACTTGTAACTCCCTTCTAATTTTGTCTCTTACAATAAACAATGA‑TTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATCCATCTTGCACCTATCAt                                                   <  4:1808015/150‑1 (MQ=255)
                                                                                                agGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGA‑TTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATCCATCTTGCACCTATCAt                                                   <  8:752391/150‑1 (MQ=255)
                                                                                                 ggggCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGA‑TTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATCCATCTTGCACCTATCATa                                                  >  5:612905/1‑150 (MQ=255)
                                                                                                     cATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGA‑TTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATCCATCTTGCACCTATCATAAAAtt                                             >  7:1678471/1‑151 (MQ=255)
                                                                                                       tACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATG‑‑ATTTTTTTTGAACGAGAAAACTCAGCTGTTTATAATTAAATCAAATGATTGACAGAATAAAACTACGGCGCAAACATAATCCATATTGCCACTATCATAAAATTaa                                           >  3:224036/1‑150 (MQ=255)
                                                                                                           ggTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGA‑TTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATCCATCTTGCACCTATCATAAAATTAAAAc                                        <  3:1889664/150‑1 (MQ=255)
                                                                                                                          aTTTTGTCGCTTACAATAAACAATGA‑TTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATCCATCTTGCACCTATCATAAAATTAAAACAAGTTGATAGTAGTCa                        <  5:9545/151‑1 (MQ=255)
                                                                                                                            tttGTCGCTTACAATAAACAATGA‑TTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATCCATCTTGCACCTATCATAAAATTAAAACAAGTTGATAGTAGTCaa                       <  8:519265/150‑1 (MQ=255)
                                                                                                                              ttacgttaaCAATAAACAATTA‑TTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATCCATCTTGCACCTATCATAAAATTAAAACAAGTTGATAGTAGTCAAAt                     <  2:1051134/142‑1 (MQ=255)
                                                                                                                                 cGCTTACAATAAACAATG‑‑ATTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATCCATCTTGCACCTATCATAAAATTAAAACAAGTTGATAGTAGTCAAATaaca                 <  5:1502821/150‑1 (MQ=255)
                                                                                                                                  gCTTACAATAAACAATGA‑TTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATCCATCTTGCACCTATCATAAAATTAAAACAAGTTGATAGTAGTCAAATaaca                 >  8:1785553/1‑150 (MQ=255)
                                                                                                                                     tACAATAAACAATGA‑TTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATCCATCTTGCACCTATCATAAAATTAAAACAAGTTGATAGTAGTCAAATaacaac               <  2:582599/149‑1 (MQ=255)
                                                                                                                                      aCAATAAACAATGA‑TTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATCCATCTTGCACCTATCATAAAATTAAAACAAGTTGATAGTAGTCAAATAACAACCaa            <  7:210129/151‑1 (MQ=255)
                                                                                                                                      aCAATAAACAATGA‑ATTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATCCATCTTGCACCTATCATAAAATTAAAACAAGTTGATAGTAGTCAAATAACAACCa             >  6:312080/1‑150 (MQ=255)
                                                                                                                                       cAATAAACAATGA‑TTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATCCATCTTGCACCTATCATAAAATTAAAACAAGTTGATAGTAGTCAAATAACAACCAAt           >  5:810431/1‑151 (MQ=255)
                                                                                                                                         aTAAACAATGA‑TTTTTTTTTGAACGAGATAACTCTCGTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATCCATCTTGCACCTATCATAAAATTAAAACAAGTTGATAGTAGTCAAATAACAACCAATTa         <  7:1537579/151‑1 (MQ=255)
                                                                                                                                            aaCAATGA‑TTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATCCATCTTGCACCTATCATAAAATTAAAACAAGTTGATAGTAGTCAAATAACAACCAATTAAAt      >  1:743591/1‑151 (MQ=255)
                                                                                                                                            aaCAATGA‑TTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATCCATCTTGCACCTATCATAAAATTAAAACAAGTTGATAGTAGTCAAATAACAACCAATTAAAt      >  8:597877/1‑151 (MQ=255)
                                                                                                                                             aCAATGA‑TTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATCCATCTTGCACCTATCATAAAATTAAAACAAGTTGATAGTAGTCAAATAACAACCAATTAAATa     <  6:1337087/151‑1 (MQ=255)
                                                                                                                                              cAATGA‑TTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATCCATCTTGCACCTATCATAAAATTAAAACAAGTTGATAGTAGTCAAATAACAACCAATTAAATac    >  7:1234115/1‑151 (MQ=255)
                                                                                                                                               aaTGA‑TTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATCCATCTTGCACCTATCATAAAATTAAAACAAGTTGATAGTAGTCAAATAACAACCAATTAAATaca   <  3:294678/151‑1 (MQ=255)
                                                                                                                                               aaTGA‑TTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATCCATCTTGCACCTATCATAAAATTAAAACAAGTTGATAGTAGTCAAATAACAACCAATTAAATaca   <  3:723157/151‑1 (MQ=255)
                                                                                                                                                aTGA‑TTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATCCATCTTGCACCTATCATAAAATTAAAACAAGTTGATAGTAGTCAAATAACAACCAATTAAATacac  >  4:1189164/1‑151 (MQ=255)
                                                                                                                                                aTGA‑TTTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATCCATCTTGCACCTATCATAAAATTAAAACAAGTTGATAGTAGTCAAATAACAACCAATTAAATacac  <  1:350542/151‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
TCTTATCAAGAGTAAATTTGCCAGGCAGCTCATTATTCACGTCGATAAAAAAGGATAAACATGTTTTCTTATCTATATCAACAATTCTTAGTTTAGAGGGGCATACTGGTAACTCCCTTCTAATTTTGTCGCTTACAATAAACAATGA‑ATTTTTTTTGAACGAGATAACTCTCCTGTTTATAATTAAATCAAATGATTGACAGATGAAAACTACGGAGCAAACATAATCCATCTTGCACCTATCATAAAATTAAAACAAGTTGATAGTAGTCAAATAACAACCAATTAAATACAC  >  DH1/3297207‑3297501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑