BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	DH1	1,299,401	G→T	A389S (GCG→TCG)	aegA →	protein AegA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	DH1	1,299,401	0	G	T	98.9%	1689.7 / 4.2	450	A389S (GCG→TCG)	aegA	protein AegA
Reads supporting (aligned to +/- strand): ref base G (4/1); new base T (204/241); total (208/242)
Fisher's exact test for biased strand distribution p-value = 1.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01

Only 100 of 459 total aligned reads displayed.

GCTGGCCTGTGCGGATGTTCTGACCCGCAATGGCGTGGGGGTGACGGTGTACGATCGCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCGCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCGTAGGCACTTACCGTTCCATGAAAGCGGGTTTACCCAATGAAGATGCGCCGGGCGTTT  >  DH1/1299253‑1299551

gCTGGCCTGTGCGGATGTTCTGACCCGCAATGGCGTGGGGGTGACGGTGTACGATCGCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGctcg                                                                                                                                                      <  3:1144169/151‑1 (MQ=255)

gCTGGCCTGTGCGGATGTTCTGACCCGCAATGGCGTGGGGGTGACGGTGTACGATCGCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGctcg                                                                                                                                                      <  6:1286512/151‑1 (MQ=255)

    gCCTGTGCGGATGTTCTGACCCGCAATGGCGTGGGGGTGACGGTGTACGATCGCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATgg                                                                                                                                                  <  6:781432/151‑1 (MQ=255)

    gCCTGTGCGGATGTTCTGACCCGCAATGGCGTGGGGGTGACGGTGTACGATCGCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATgg                                                                                                                                                  <  4:324074/151‑1 (MQ=255)

    gCCTGTGCGGATGTTCTGACCCGCAATGGCGTGGGGGTGACGGTGTACGATCGCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATgg                                                                                                                                                  <  1:646667/151‑1 (MQ=255)

     ccTGTGCGGATGTTCTGACCCGCAATGGCGTGGGGGTGACGGTGTACGATCGCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATggg                                                                                                                                                 >  1:1264437/1‑151 (MQ=255)

     ccTGTGCGGATGTTCTGACCCGCAATGGCGTGGGGGTGACGGTGTACGATCGCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATggg                                                                                                                                                 >  3:776715/1‑151 (MQ=255)

     ccTGTGCGGATGTTCTGACCCGCAATGGCGTGGGGGTGACGGTGTACGATCGCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATggg                                                                                                                                                 >  8:1875780/1‑151 (MQ=255)

      cTGTGCGGATGTTCTGACCCGCAATGGCGTGGGGGTGACGGTGTACGATCGCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATgggg                                                                                                                                                >  5:875685/1‑151 (MQ=255)

        gtgCTGATGTTCTGACCAGCAATGGCGTGGGGGTGACGGTGTACGATCGCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATgggg                                                                                                                                                <  8:1642978/149‑1 (MQ=255)

         tgCGGATGTTCTGACCCGCAATGGCGTGGGGGTGACGGTGTACGATCGCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGAtt                                                                                                                                             >  4:1238368/1‑151 (MQ=255)

          gCGGATGTTCTGACCCGCAATGGCGTGGGGGTGACGGTGTACGATCGCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGAtt                                                                                                                                             >  8:463523/1‑150 (MQ=255)

          gCGGATGTTCTGACCCGCAATGGCGTGGGGGTGACGGTGTACGATCGCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCATCAGCTCGATGGGGATTc                                                                                                                                            >  6:199675/1‑151 (MQ=255)

           cGGATGTTCTGACCCGCAATGGCGTGGGGGTGACGGTGTACGATCGCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTc                                                                                                                                            >  1:1548104/1‑150 (MQ=255)

                  tCTGACCCGCAATGGCGTGGGGGTGACTGTGTACTATCGCCATCCAGAAATCGGTGGCTTTCTCACTTTCGGCATTCCTTCTTTGAAACTGTATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCGCGATGTGGATTCACTTCGa                                                                                                                                     <  4:168629/150‑1 (MQ=255)

                     gACCCGCAATGGCGTGGGGGTGACGGTGTACGATCGCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATCGGGATTCACTTCGAACt                                                                                                                                  >  3:522707/1‑150 (MQ=255)

                         cGCAATGGCGTGGGGGTGACGGTGTACGATCGCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAAtt                                                                                                                             <  5:105190/151‑1 (MQ=255)

                         cGCAATGGCGTGGGGGTGACGGTGTACGATCGCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAAtt                                                                                                                             >  3:1318305/1‑151 (MQ=255)

                          gCAATGGCGTGGGGGTGACGGTGTACGATCGCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGCAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATtg                                                                                                                            >  5:1946871/1‑151 (MQ=255)

                             aTGGCGTGGGGGTGACGGTGTACGATCGCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGa                                                                                                                         <  7:1169301/151‑1 (MQ=255)

                               ggCGTGGGGGTGACGGTGTACGATCGCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGaa                                                                                                                        <  1:784508/150‑1 (MQ=255)

                                gCGTGGGGGTGACGGTGTACGATCGCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGt                                                                                                                      <  2:1355491/151‑1 (MQ=255)

                                  gTGGGGGTGACGGTGTACGATCGCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTgg                                                                                                                    >  4:803626/1‑151 (MQ=255)

                                    gggggTGACGGTGTACGATCGCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGt                                                                                                                  <  6:913651/151‑1 (MQ=255)

                                     ggggTGACGGTGTACGATCGCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGt                                                                                                                  >  6:1525699/1‑150 (MQ=255)

                                      gggTGACGGTGTACGATCGCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTaa                                                                                                                <  5:1477690/151‑1 (MQ=255)

                                         tGACGGTGTACGATCGCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTaa                                                                                                                >  7:1325289/1‑148 (MQ=255)

                                          gACGGTGTACGATCGCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGa                                                                                                             <  7:940823/150‑1 (MQ=255)

                                          gACGGTGTACGATCGCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGAt                                                                                                            <  7:323362/151‑1 (MQ=255)

                                          gACGGTGTACGATCGCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGAt                                                                                                            <  1:235893/151‑1 (MQ=255)

                                          gACGGTGTACGATCGCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGAt                                                                                                            <  8:1279241/151‑1 (MQ=255)

                                              gtgtACGATCGCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGtct                                                                                                        >  2:1090316/1‑151 (MQ=255)

                                                gtACGATCGCCATCGAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTGGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGtctc                                                                                                       <  4:617480/150‑1 (MQ=255)

                                                gtACGATCGCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTctct                                                                                                      >  5:1798183/1‑151 (MQ=255)

                                                  aCGATCGCCATCCAGAAATCGGGGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCttt                                                                                                    <  4:1812875/151‑1 (MQ=255)

                                                    gATCGCCATGCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTgg                                                                                                  <  5:1339188/151‑1 (MQ=255)

                                                    gATCGCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTgg                                                                                                  <  1:322072/151‑1 (MQ=255)

                                                     aTCGCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTgg                                                                                                  >  8:1104479/1‑150 (MQ=255)

                                                     aTCGCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGa                                                                                                 <  3:645276/151‑1 (MQ=255)

                                                       cGCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGAtt                                                                                               <  2:139882/151‑1 (MQ=255)

                                                          cATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGc                                                                                            >  5:347471/1‑151 (MQ=255)

                                                               aGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTg                                                                                       >  4:1022452/1‑151 (MQ=255)

                                                                gAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTgg                                                                                      <  1:919307/151‑1 (MQ=255)

                                                                 aaaTCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTgg                                                                                      >  2:944623/1‑150 (MQ=255)

                                                                   aTCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAAc                                                                                   <  8:1403103/151‑1 (MQ=255)

                                                                   aTCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAAc                                                                                   <  7:784765/151‑1 (MQ=255)

                                                                       gTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATa                                                                               <  1:1952074/151‑1 (MQ=255)

                                                                        tGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATac                                                                              >  6:1179794/1‑151 (MQ=255)

                                                                         ggCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATacg                                                                             <  7:703063/151‑1 (MQ=255)

                                                                            ttGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATacgacg                                                                          >  8:320101/1‑151 (MQ=255)

                                                                             tGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATacgacg                                                                          <  8:270609/150‑1 (MQ=255)

                                                                             tGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGAcgc                                                                         >  6:426934/1‑151 (MQ=255)

                                                                               cTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCgg                                                                       >  4:245829/1‑151 (MQ=255)

                                                                                 cACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTAGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGAAGCGGTc                                                                     >  1:1008114/1‑151 (MQ=255)

                                                                                       cGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGtt                                                               >  6:500310/1‑151 (MQ=255)

                                                                                       cGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGtt                                                               <  6:634890/151‑1 (MQ=255)

                                                                                        ggCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTg                                                              >  6:1661940/1‑151 (MQ=255)

                                                                                          cATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGc                                                            <  3:206148/151‑1 (MQ=255)

                                                                                          cATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTCGGAACAATACGACGCGGTCTTCGTTGGc                                                            <  7:1612123/151‑1 (MQ=255)

                                                                                            ttCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCGt                                                          >  6:258619/1‑151 (MQ=255)

                                                                                            ttCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCGt                                                          >  4:1618368/1‑151 (MQ=255)

                                                                                            ttCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCGt                                                          >  1:137548/1‑151 (MQ=255)

                                                                                             tCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCGt                                                          >  5:739903/1‑150 (MQ=255)

                                                                                               cttcttTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCGTAgg                                                       >  7:402011/1‑151 (MQ=255)

                                                                                                ttcttTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCGTAGGc                                                      >  5:583274/1‑151 (MQ=255)

                                                                                                ttcttTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCGTAGGc                                                      >  1:747544/1‑151 (MQ=255)

                                                                                                 tcttTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCGTAgg                                                       <  8:841103/149‑1 (MQ=255)

                                                                                                        aaCTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCGTAGGCACTTACCg                                              <  1:148339/151‑1 (MQ=255)

                                                                                                        aaCTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCGTAGGCACTTACCg                                              <  4:393214/151‑1 (MQ=255)

                                                                                                        aaCTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCGTAGGCACTTACCg                                              >  3:390678/1‑151 (MQ=255)

                                                                                                         aCTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCGTAGGCACTTACCGt                                             >  2:124035/1‑151 (MQ=255)

                                                                                                          cTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCGTAGGCACTTACCGtt                                            >  7:232977/1‑151 (MQ=255)

                                                                                                              aTAAATCCCTGCTGGCACGCCGTCGGTAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCGTAGGCACTTACCTTTCCAt                                        <  7:1870529/151‑1 (MQ=255)

                                                                                                                aaaTCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTCGAACAATACGACCCGGACTTCGTTGGCGTAGGCACATACCGTTCCATg                                       >  2:29078/1‑150 (MQ=255)

                                                                                                                 aaTCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCGTAGGCACTTACCGTTCCATGaa                                     <  1:1085822/151‑1 (MQ=255)

                                                                                                                   tCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCGTAGGCACTTACCGTTCCATGaa                                     >  2:203409/1‑149 (MQ=255)

                                                                                                                    cccTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCGTAGGCACTTACCGTTCCATGAAAg                                   <  2:1720896/150‑1 (MQ=255)

                                                                                                                    cccTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCGTAGGCACTTACCGTTCCATGAAAGc                                  >  1:1313642/1‑151 (MQ=255)

                                                                                                                     ccTGCTGGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCGTAGGCACTTACCGTTCCATGAAAGCg                                 <  1:483391/151‑1 (MQ=255)

                                                                                                                        gctgGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCGTAGGCACTTACCGTTCCATGAAAGCgg                                <  7:1923210/149‑1 (MQ=255)

                                                                                                                        gctgGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCGTAGGCACTTACCGTTCCATGAAAGCgg                                <  1:1706184/149‑1 (MQ=255)

                                                                                                                        gctgGCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCGTAGGCACTTACCGTTCCATGAAAGCGGGt                              <  5:1627563/151‑1 (MQ=255)

                                                                                                                           ggCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCGTAGGCACTTACCGTTCCATGAAAGCGGGtt                             <  5:1742853/149‑1 (MQ=255)

                                                                                                                           ggCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCGTAGGCACTTACCGTTCCATGAAAGCGGGtt                             <  1:1832547/149‑1 (MQ=255)

                                                                                                                           ggCACGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCGTAGGCACTTACCGTTCCATGAAAGCGGGTTTa                           <  2:1020531/151‑1 (MQ=255)

                                                                                                                               cGCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCGTAGGCACTTACCGTTCCATGAAAGCGGGTTTAccc                        >  2:1756006/1‑150 (MQ=255)

                                                                                                                                gCCGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCGTAGGCACTTACCGTTCCATGAAAGCGGGTTTAccaa                       >  6:1886345/1‑148 (MQ=255)

                                                                                                                                  cGTCGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCGTAGGCACTTACCGTTCCATGAAAGCGGGTTTACCCAATg                    >  7:1433719/1‑151 (MQ=255)

                                                                                                                                     cGGGAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCGTAGGCACTTACCGTTCCATGAAAGCGGGTTTACCCAATGa                   >  1:1903326/1‑149 (MQ=255)

                                                                                                                                        gAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCGTAGGCACTTACCGTTCCATGAAAGCGGGTTTACCCAATGAAGATg              <  5:1207279/151‑1 (MQ=255)

                                                                                                                                        gAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCGTAGGCACTTACCGTTCCATGAAAGCGGGTTTACCCAATGAAGATg              <  7:610538/151‑1 (MQ=255)

                                                                                                                                        gAAATCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCGTAGGCACTTACCGTTCCATGAAAGCGGGTTTACCCAATGAAGATg              <  3:965998/151‑1 (MQ=255)

                                                                                                                                         aaaTCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCGTAGGCACTTACCGTTCCATGAAAGCGGGTTTACCCAATGAAGATgc             >  7:9949/1‑151 (MQ=255)

                                                                                                                                          aaTCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCGTAGGCACTTACCGTTCCATGAAAGCGGGTTTACCCAATGAAGATgcg            <  3:1594036/151‑1 (MQ=255)

                                                                                                                                           aTCTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCGTAGGCACTTACCGTTCCATGAAAGCGGGTTTACCCAATGAAGATgcgc           >  8:55854/1‑151 (MQ=255)

                                                                                                                                             cTTCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCGTAGGCACTTACCGTTCCATGAAAGCGGGTTTACCCAATGAAGATGCGCCg         >  2:1633571/1‑151 (MQ=255)

                                                                                                                                               tCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCGTAGGCACTTACCGTTCCATGAAAGCGGGTTTACCCAATGATGATGCGCCggg       >  2:75771/1‑151 (MQ=255)

                                                                                                                                               tCAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCGTAGGCACTTACCGTTCCATGAAAGCGGGTTTACCCAATGAAGATGCGCCggg       >  2:56429/1‑151 (MQ=255)

                                                                                                                                               tCAGCGCGATGGGGATTCACTTCGAACTCGATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCGTAGGCACTTACCGTTCCATGAAAGCGGGTTTACCCAATGAAGATGCGCCggg       >  3:1810977/1‑151 (MQ=255)

                                                                                                                                                cAGCTCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCGTAGGCACTTACCGTTCCATGAAAGCGGGTTTACCCAATGAAGATGCGCCggg       <  8:1504973/150‑1 (MQ=255)

GCTGGCCTGTGCGGATGTTCTGACCCGCAATGGCGTGGGGGTGACGGTGTACGATCGCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCAAACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCGCGATGGGGATTCACTTCGAACTCAATTGTGAAGTGGGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCGTAGGCACTTACCGTTCCATGAAAGCGGGTTTACCCAATGAAGATGCGCCGGGCGTTT  >  DH1/1299253‑1299551

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`