Predicted mutation
evidence seq id position mutation annotation gene description
RA DH1 4,414,695 G→A A251T (GCA→ACA)  rhaT → l‑rhamnose‑proton symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH14,414,6950GA98.2% 2039.3 / 12.5 561A251T (GCA→ACA) rhaTl‑rhamnose‑proton symporter
Reads supporting (aligned to +/- strand):  ref base G (6/4);  new base A (284/266);  total (291/270)
Fisher's exact test for biased strand distribution p-value = 7.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.77e-01

Only 100 of 561 total aligned reads displayed.

CGCTGCCGCACTTGGCGTCGATCCACTGTATGTCGCTCTGCCAAGCTATGTTGTCATCATGGGCGGCGGCGCGATCATTAACCTCGGTTTCTGTTTTATTCGTCTGGCAAAAGTGAAGGATTTGTCGCTAAAAGCCGACTTCTCGCTGGCAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTGGTATCTGCAATTCTTTTTCTATGCCTGGGGCCACGCCCGCATTCCGGCGCAGTATGACTACATCAGTTGGATGCTGCATATGAGTTTCTATG  >  DH1/4414547‑4414842
                                                                                                                                                    |                                                                                                                                                   
   tGCCGCACTTGGCGTCGATCCACTGTATGTCGCTCTGCCAAGCTATGTTGTCATCATGGGCGGCGGCGCGATCATTAACCTCGGTTTCTGTTTTATTCGTCTGGCAAAAGTGAAGGATTTGTCGCTAAAAGCCGACTTCTCGCTGACaaa                                                                                                                                                 >  5:1676446/1‑150 (MQ=255)
    gCCGCACTTGGCGTCGATCCACTGTCTGTCGCTCTGCCAAGCTATGTTGTCATCATGGGCGGCGGCGCGATCATTAACCTCGGTTTCTGTTTTATTCGTCTGGCAAAAGTGAAGGATTTGTCGCTAAAAGCCGACTTCTCGCTGACaaaa                                                                                                                                                <  6:903644/150‑1 (MQ=255)
     ccGCACTTGGCGTCGATCCACTGTATGTCGCTCTGCCAAGCTATGTTGTCATCATGGGCGGCGGCGCGATCATTAACCTCGGTTTCTGTTTTATTCGTCTGGCAAAAGTGAAGGATTTGTCGCTAAAAGCCGACTTCTCGCTGACAAAATc                                                                                                                                              >  4:496978/1‑151 (MQ=255)
           ttGGCGTCGATCCACTGTATGTCGCTCTGCCAAGCTATGTTGTCATCATGGGCGGCGGCGCGATCATTAACCTCGGTTTCTGTTTTATTCGTCTGGCAAAAGTGAAGGATTTGTCGCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGAt                                                                                                                                        >  8:490743/1‑151 (MQ=255)
            tGGCGTCGATCCACTGTATGTCGCTCTGCCAAGCTATGTTGTCATCATGGGCGGCGGCGCGATCATTAACCTCGGTTTCTGTTTTATTCGTCTGGCAAAAGTGAAGGATTTGTCGCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGAt                                                                                                                                        <  6:385124/150‑1 (MQ=255)
            tGGCGTCGATCCACTGTATGTCGCTCTGCCAAGCTATGTTGTCATCATGGGCGGCGGCGCGATCATTAACCTCGGTTTCTGTTTTATTCGTCTGGCAAAAGTGAAGGATTTGTCGCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATc                                                                                                                                       >  3:1395470/1‑151 (MQ=255)
                  cGATCCACTGTATGTCGCTCTGCCAAGCTATGTTGTCATCATGGGCGGCGGCGCGATCATTAACCTCGGTTTCTGTTTTATTCGTCTGGCAAAAGTGAAGGATTTGTCGCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTcac                                                                                                                                 >  7:381454/1‑151 (MQ=255)
                  cGATCCACTGTATGTCGCTCTGCCAAGCTATGTTGTCATCATGGGCGGCGGCGCGATCATTAACCTCGGTTTCTGTTTTATTCGTCTGGCAAAAGTGAAGGATTTGTCGCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTcac                                                                                                                                 >  3:29559/1‑151 (MQ=255)
                  cGATCCACTGTATGTCGCTCTGCCAAGCTATGTTGTCATCATGGGCGGCGGCGCGATCATTAACCTCGGTTTCTGTTTTATTCGTCTGGCAAAAGTGAAGGATTTGTCGCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTcac                                                                                                                                 <  3:407111/151‑1 (MQ=255)
                   gATCCACTGTATGTCGCTCTGCCAAGCTATGTTGTCATCATGGGCGGCGGCGCGATCATTAACCTCGGTTTCTGTTTTATTCGTCTGGCAAAAGTGAAGGATTTGTCGCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTcaca                                                                                                                                <  6:1970743/151‑1 (MQ=255)
                      ccACTGTATGTCGCTCTGCCAAGCTATGTTGTCATCATGGGCGGCGGCGCGGTCATTAACCTCGGTTTCTGTTTTATTCGTCTGGCAAAAGTGAAGGATTTGTCGCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAAtg                                                                                                                             <  8:557497/151‑1 (MQ=255)
                      ccACTGTATGTCGCTCTGCCAAGCTATGTTGTCATCATGGGCGGCGGCGCGATCATTAACCTCGGTTTCTGTTTTATTCGTCTGGCAAAAGTGAAGGATTTGTCGCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAAtg                                                                                                                             >  6:144923/1‑151 (MQ=255)
                         cTGTATGTCGCTCTGCCAAGCTATGTTGTCATCATGGGCGGCGGCGCGATCATTAACCTCGGTTTCTGTTTTATTCGTCTGGCAAAAGTGAAGGATTTGTCGCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAATgtgt                                                                                                                          >  6:1112592/1‑151 (MQ=255)
                                 cGCTCTGCCAAGCTATGTTGTCATCATGGGCGGCGGCGCGATCATTAACCTCGGTTTCTGTTTTATTCGTCTGGCAAAAGTGAAGGATTTGTCGCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACtctc                                                                                                                   <  2:314940/150‑1 (MQ=255)
                                       gCCAAGCTATGTTGTCATCATGGGCGGCGGCGCGATCATTAACCTCGGTTTCTGTTTTATTCGTCTGGCAAAAGTGAAGGATTTGTCGCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACt                                                                                                             <  5:1349746/150‑1 (MQ=255)
                                        ccAAGCTATGTTGTCATCATGGGCGGCGGCGCGATCATTAACCTCGGTTTCTGTTTTATTCGTCTGGCAAAAGTGAAGGATTTGTCGCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTgg                                                                                                           >  7:678248/1‑151 (MQ=255)
                                        ccAAGCTATGTTGTCATCATGGGCGGCGGCGCGATCATTAACCTCGGTTTCTGTTTTATTCGTCTGGCAAAAGTGAAGGATTTGTCGCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTgg                                                                                                           <  7:1708521/151‑1 (MQ=255)
                                          aaGCTATGTTGTCATCATGGGCGGCGGCGCGATCATTAACCTCGGTTTCTGTTTTATTCGTCTGGCAAAAGTGAAGGATTTGTCGCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGc                                                                                                         >  1:1020161/1‑151 (MQ=255)
                                            gCTATGTTGTCATCATGGGCGGCGGCGCGATCATTAACCTCGGTTTCTGTTTTATTCGTCTGGCAAAAGTGAAGGATTTGTCGCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCgg                                                                                                       <  5:850797/151‑1 (MQ=255)
                                             cTATGTTGTCATCATGGGCGGCGGCGCGATCATTAACCTCGGTTTCTGTTTTATTCGTCTGGCAAAAGTGAAGGATTTGTCGCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCggg                                                                                                      >  5:1135520/1‑151 (MQ=255)
                                               aTGTTGTCATCATGGGCGGCGGCGCGATCATTAACCTCGGTTTCTGTTTTATTCGTCTGGCAAAAGTGAAGGATTTGTCGCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGtt                                                                                                    <  6:31327/151‑1 (MQ=255)
                                                 gttgtCATCATGGGCGGCGGCGCGATCATTAACCTCGGTTTCTGTTTTATTCGTCTGGCAAAAGTGAAGGATTTGTCGCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTg                                                                                                   >  2:1177496/1‑150 (MQ=255)
                                                 gttgtCATCATGGGCGGCGGCGCGATCATTAACCTCGGTTTCTGTTTTATTCGTCTGGCAAAAGTGAAGGATTTGTCGCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTg                                                                                                   >  3:1888353/1‑150 (MQ=255)
                                                 gttgtCATCATGGGCGGCGGCGCGATCATTAACCTCGGTTTCTGTTTTATTCGTCTGGCAAAAGTGAAGGATTTGTCGCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGa                                                                                                  <  6:226554/151‑1 (MQ=255)
                                                   tgtCATCATGGGCGGCGGCGCGATCATTAACCTCGGTTTCTGTTTTATTCGTCTGGCAAAAGTGAAGGATTTGTCGCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGAtg                                                                                                >  2:679603/1‑151 (MQ=255)
                                                     tcatcaTGGGCGGCGGCGCGATCATTAACCTCGGTTTCTGTTTTATTCGTCTGGCAAAAGTGAAGGATTTGTCGCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTAATCTCGACACTGGGCGGGTTGAtgtg                                                                                              >  1:1638284/1‑151 (MQ=255)
                                                     tcatcaTGGGCGGCGGCGCGATCATTAACCTCGGTTTCTGTTTTATTCGTCTCGCAAAAGTGAAGGATTTGTCGCTAAAAGCCGAATTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGCCACTGGGCGGGATGAtgtg                                                                                              >  8:1706687/1‑151 (MQ=255)
                                                         catGGGCGGCGGCGCGATCATTAACCTCGGTTTCTGTTTTATTCGTCTGGCAAAAGTGAAGGATTTGTCGCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTGGTAt                                                                                          <  3:1203880/151‑1 (MQ=255)
                                                          atGGGCGGCGGCGCGATCATTAACCTCGGTTTCTGTTTTATTCGTCTGGCAAAAGTGAAGGATTTGTCGCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTGGTATc                                                                                         >  4:594896/1‑151 (MQ=255)
                                                            gggCGGCGGCGCGATCATTAACCTCGGTTTCTGTTTTATTCGTCTGGCAAAAGTGAAGGATTTGTCGCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTGGTATCt                                                                                        <  8:1057114/150‑1 (MQ=255)
                                                            gggCGGCGGCGCGATCATTAACCTCGGTTTCTGTTTTATTCGTCTGGCAAAAGTGAAGGATTTGTCGCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTGGTATCTg                                                                                       >  2:1490075/1‑151 (MQ=255)
                                                            gggCGGCGGCGCGATCATTAACCTCGGTTTCTGTTTTATTCGTCTGGCAAAAGTGAAGGATTTGTCGCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTGGTATCTg                                                                                       <  3:1466666/151‑1 (MQ=255)
                                                                ggcggcgCGATCATTAACCTCGGTTTCTGTTTTATTCGTCTGGCAAAAGTGAAGGATTTGTCGCTAAAAGCCGACTTCTCGCTGGCAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTGGTATCTGCAAt                                                                                   <  4:1685056/151‑1 (MQ=255)
                                                                ggcggcgCGATCATTAACCTCGGTTTCTGTTTTATTCGTCTGGCAAAAGTGAAGGATTTGTCGCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTGGTATCTGCAAt                                                                                   >  4:1265708/1‑151 (MQ=255)
                                                                ggcggcgCGATCATTAACCTCGGTTTCTGTTTTATTCGTCTGGCAAAAGTGAAGGATTTGTCGCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTGGTATCTGCAAt                                                                                   >  7:1857774/1‑151 (MQ=255)
                                                                   ggcgCGATCATTAACCTCGGTTTCTGTTTTATTCGTCTGGCAAAAGTGAAGGATTTGTCGCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTGGTATCTGCAATTCt                                                                                <  6:1764388/151‑1 (MQ=255)
                                                                         aTCATTAACCTCGGTTTCTGTTTTATTCGTCTGGCAAAAGTGAAGGATTTGTCGCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTGGTATCTGCAATTCTTTTTCt                                                                          >  1:1138091/1‑151 (MQ=255)
                                                                         aTCATTAACCTCGGTTTCTGTTTTATTCGTCTGGCAAAAGTGAAGGATTTGTCGCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTGGTATCTGCAATTCTTTTTCt                                                                          >  1:568408/1‑151 (MQ=255)
                                                                         aTCATTAACCTCGGTTTCTGTTTTATTCGTCTGGCAAAAGTGAAGGATTTGTCGCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTGGTATCTGCAATTCTTTTTCt                                                                          <  3:1003316/151‑1 (MQ=255)
                                                                           cATTAACCTCGGTTTCTGTTTTATTCGTCTGGCAAAAGTGAAGGATTTGTCGCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTGGTATCTGCAATTCTTTTTCTAt                                                                        <  3:953915/151‑1 (MQ=255)
                                                                             ttAACCTCGGTTTCTGTTTTATTCGTCTGGCAAAAGTGAAGGATTTGTCGCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTGGTATCTGCAATTCTTTTTCTATGc                                                                      >  4:266485/1‑151 (MQ=255)
                                                                              tAACCTCGGTTTCTGTTTTATTCGTCTGGCAAAAGTGAAGGATTTGTCGCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTGGTATCTGCAATTCTTTTTCTATGcc                                                                     >  8:1863983/1‑151 (MQ=255)
                                                                              tAACCTCGGTTTCTGTTTTATTCGTCTGGCAAAAGTGAAGGATTTGTCGCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTGGTATCTGCAATTCTTTTTCTATGcc                                                                     >  1:1934425/1‑151 (MQ=255)
                                                                               aaCCTCGGTTTCTGTTTTATTCGTCTGGCAAAAGTGAAGGATTTGTCGCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTCGTATCTGCAATTCTTTTTCTATGCCt                                                                    <  6:1680279/151‑1 (MQ=255)
                                                                                  cTCGGTTTCTGTTTTATTCGTCTGGCAAAAGTGAAGGATTTGTCGCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTGGTATCTGCAATTCTTTTTCTATGCCTggg                                                                 >  1:1890343/1‑151 (MQ=255)
                                                                                    cGGTTTCTGTTTTATTCGTCTAGCAAAAGTGAAGGATTTGTCGCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTGGTATCTGCAATTCTTTTTCTATGCCTGGGGc                                                               >  1:382190/1‑151 (MQ=255)
                                                                                       tttCTGTTTTATTCGTCTGGCAAAAGTGAAGGATTTGTCGCTAAAAGCCGACTTCTCGCTGACAAAATAGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTGGTATCTGCAATTATTTTTCTATGCCTGGGGCCAc                                                            >  5:908889/1‑151 (MQ=255)
                                                                                        ttCTGTTTTATTCGTCTGGCAAAAGTGAAGGATTTGTCGCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTGGTATCTGCAATTCTTTTTCTATGCCTGGGGCCACg                                                           >  3:1643354/1‑151 (MQ=255)
                                                                                          cTGTTTTATTCGTCTGGCAAAAGTGAAGGATTTGTCGCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTGGTATCTGCAATTCTTTTTCTATGCCTGGGGCCACGcc                                                         >  2:766191/1‑151 (MQ=255)
                                                                                            gTTTTATTCGTCTGGCAAAAGTGAAGGATTTGTCGCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTGGTATCTGCAATTCTTTTTCTATGCCTGGGGCCACGcc                                                         <  7:296978/149‑1 (MQ=255)
                                                                                            gTTTTATTCGTCTGGCAAAAGTGAAGGATTTGTCGCTAAAAGCCGACTTCTCGCCGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTGGTATCTGCAATTCTTTTTCTATGCCTGGGGCCACGCCCg                                                       >  6:623241/1‑151 (MQ=255)
                                                                                               ttATTCGTCTGGCAAAAGTGAAGGATTTGTCGCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTGGTATCTGCAATTCTTTTTCTATGCCTGGGGCCACGCCCGCa                                                     <  5:1540505/150‑1 (MQ=255)
                                                                                                tATTCGTCTGGCAAAAGTGAAGGATTTGTCGCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTGGTATCTGCAATTCTTTTTCTATGCCTGGGGCCACGCCCGCAtt                                                   >  4:174274/1‑151 (MQ=255)
                                                                                                 aTTCGTCTGGCAAAAGTGAAGGATTTGTCGCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTGGTATCTGCAATTCTTTTTCTATGCCTGGGGCCACGCCCGCAtt                                                   <  4:1403177/150‑1 (MQ=255)
                                                                                                 aTTCGTCTGGCAAAAGTGAAGGATTTGTCGCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTGGTATCTGCAATTCTTTTTCTATGCCTGGGGCCACGCCCGCAtt                                                   <  5:377923/150‑1 (MQ=255)
                                                                                                 aTTCGTCTGGCAAAAGTGAAGGATTTGTCGCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTGGTATCTGCAATTCTTTTTCTATGCCTGGGGCCACGCCCGCAtt                                                   <  4:908080/150‑1 (MQ=255)
                                                                                                  ttCGTCTGGCAAAAGTGAAGGATTTGTCGCTAAAAGCCGACTTCTCGCTGCCAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTGGTATCTGCAATTCTTTTTCTATGCCTGGGGCCACGCCCGCATTcc                                                 >  7:478623/1‑151 (MQ=255)
                                                                                                   tCGTCTGGCAAAAGTGAAGGATTTGTCGCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTGGTATCTGCAATTCTTTTTCTATGCCTGGGGCCACGCCCGCATTcc                                                 <  3:688463/150‑1 (MQ=255)
                                                                                                   tCGTCTGGCAAAAGTGAAGGATTTGTCGCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTGGTATCTGCAATTCTTTTTCTATGCCTGGGGCCACGCCCGCATTcc                                                 <  6:1321988/150‑1 (MQ=255)
                                                                                                   tCGTCTGGCAAAAGTGAAGGAGTTGTCGCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTGGTATCTGCAATTCTTTTTCTATGCCTGGGGCCACGCCCGCATTcc                                                 <  2:422581/150‑1 (MQ=255)
                                                                                                    cGTCTGGCAAAAGTGAAGGATTTGTCGCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTGGTATCTGCAATTCTTTTTCTATGCCTGGGGCCACGCCCGCATTCCgg                                               >  3:54118/1‑151 (MQ=255)
                                                                                                        tGGCAAAAGTGAAGGATTTGTCGCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTGGTATCTGCAATTCTTTTTCTATGCCTGGGGCCACGCCCGCATTCCGgcgc                                            >  4:1869581/1‑150 (MQ=255)
                                                                                                        tGGCAAAAGTGAAGGATTTGTCGCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTGGTATCTGCAATTCTTTTTCTATGCCTGGGGCCACGCCCGCATTCCGgcgc                                            >  5:555581/1‑150 (MQ=255)
                                                                                                         ggCAAAAGTGAAGGATTTGTCGCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGCCACTGGGCGGGTTGATGTGGTATCTGCAATTCTTTTTCTATGCCTGGGGCCACGCCCGCATTCCGgcgc                                            >  7:1131369/1‑149 (MQ=255)
                                                                                                          gCAAAAGTGAAGGATTTGTCGCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTGGTATCTGCAATTCTTTTTCTATGCCTGGGGCCACGCCCGCATTCCGGCGCAGt                                         <  7:215337/151‑1 (MQ=255)
                                                                                                           cAAAAGTGAAGGATTTGTCGCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTGGTATCTGCAATTCTTTTTCTATGCCTGGGGCCACGCCCGCATTCCGGCGCAGTa                                        <  5:1849395/151‑1 (MQ=255)
                                                                                                            aaaaGTGAAGGATTTGTCGCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTGGTATCTGCAATTCTTTTTCTATGCCTGGGGCCACGCCCGCATTCCGGCGCAGTAt                                       >  8:231513/1‑151 (MQ=255)
                                                                                                            aaaaGTGAAGGATTTGTCGCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTGGTATCTGCAATTCTTTTTCTATGCCTGGGGCCACGCCCGCATTCCGGCGCAGTAt                                       >  3:838266/1‑151 (MQ=255)
                                                                                                            aaaaGTGAAGGATTTGTCGCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTGGTATCTGCAATTCTTTTTCTATGCCTGGGGCAACGCCCGCAATCCGGCGCAGTAt                                       >  5:1013435/1‑151 (MQ=255)
                                                                                                             aaaGTGAAGGATTTGTCGCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTGGTATCTGCAATTCTTTTTCTATGCCTGGGGCCACGCCCGCATTCCGGCGCAGTATg                                      <  1:1447535/151‑1 (MQ=255)
                                                                                                               aGTGAAGGATTTGTCGCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTGGTATCTGCAATTCTTTTTCTATGCCTGGGGCCACGCCCGCATTCCGGCGCAGTATGAc                                    >  8:1241607/1‑151 (MQ=255)
                                                                                                                gTGAAGGATTTGTCGCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTGGTATCTGCAATTCTTTTTCTATGCCTGGGGCCACGCCCGCATTCCGGCGCAGTATGACt                                   >  4:1145904/1‑151 (MQ=255)
                                                                                                                 tGAAGGATTTGTCGCTAAAAGCCGACTTCTCGCTGGCAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTGGTATCTGCAATTCTTTTTCTATGCCTGGGGCCACGCCCGCATTCCGGCGCAGTATGACt                                   >  7:1020091/1‑150 (MQ=255)
                                                                                                                  gAAGGATTTGTCGCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTGGTATCTGCAATTCTTTTTCTATGCCTGGGGCCACGCCCGCATTCCGGCGCAGTATGACTAc                                 <  2:1295818/151‑1 (MQ=255)
                                                                                                                   aaGGATTTGTCGCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTGGTATCTGCAATTCTTTTTCTATGCCTGGGGCCACGCCCGCATTCCGGCGCAGTATGACTAc                                 >  1:837884/1‑150 (MQ=255)
                                                                                                                     ggATTTGTCGCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTGGTATCTGCAATTCTTTTTCTATGCCTGGGGCCACGCCCGCATTCCGGCGCAGTATGACTACATc                              >  7:1545974/1‑151 (MQ=255)
                                                                                                                      gATTTGTCGCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTGGTATCTGCAATTCTTTTTCTATGCCTGGGGCAACGCCCGCATTCCGGCGCAGTATGACTACATc                              >  1:790480/1‑150 (MQ=255)
                                                                                                                        tttGTCGCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTGGTATCTGCAATTCTTTTTCTATGCCTGGGGCCACGCCCGCATTCCGGCGCAGTATGACTACATCAGt                           >  1:651765/1‑151 (MQ=255)
                                                                                                                        tttGTCGCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTGGTATCTGCAATTCTTTTTCTATGCCTGGGGCCACGCCCGCATTCCGGCGCAGTATGACTACATCAGt                           >  5:674675/1‑151 (MQ=255)
                                                                                                                        tttGTCGCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTGGTATCTGCAATTCTTTTTCTATGCCTGGGGCCACGCCCGCATTCCGGCGCAGTATGACTACATCAGt                           >  7:1255394/1‑151 (MQ=255)
                                                                                                                           gTCGCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTGGTATCTGCAATTCTTTTTCTATGCCTGGGGCCACGCCCGCATTCCGGCGCAGTATGACTACATCAGTTgg                        >  7:985967/1‑151 (MQ=255)
                                                                                                                             cgatAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTGGTATCTGCAATTCTTTTTCTATGCCTGGGGCCACGCCCGCATTCCGGCGCAGTATGACTAGATCAGTTGGAt                      <  8:675464/148‑1 (MQ=255)
                                                                                                                              gCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTGGTATCTGCAATTCTTTTTCTATGCCTGGGGCCACGCCCGCATTCCGGCGCAGTATGACTACATCAGTTGGAtg                     >  1:942631/1‑151 (MQ=255)
                                                                                                                              gCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTGGTATCTGCAATTCTTTTTCTATGCCTGGGGCCACGCCCGCATTCCGGCGCAGTATGACTACATCAGTTGGAtg                     <  2:966264/151‑1 (MQ=255)
                                                                                                                              gCTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTGGTATCTGCAATTCTTTTTCTATGCCTGGGGCCACGCCCGCATTCCGGCGCAGTATGACTACATCAGTTGGAtg                     >  2:1062121/1‑151 (MQ=255)
                                                                                                                               cTAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTGGTATCTGCAATTCTTTTTCTATGCCTGGGGCCACGCCCGCATTCCGGCGCAGTATGACTACATCAGTTGGAtgc                    >  8:1480456/1‑151 (MQ=255)
                                                                                                                                tAAAAGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTGGTATCTGCAATTCTTTTTCTATGCCTGGGGCCACGACCGCATTCCGGCGCAGTATGACTACATCAGTTGGAtgat                   >  2:469552/1‑149 (MQ=255)
                                                                                                                                 aaaaGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTGGTATCTGCAATTCTTTTTCTATGCCTGGGGCCACGCCCGCATTCCGGCGCAGTATGACTACATCAGTTGGAtgctg                  <  6:1612057/151‑1 (MQ=255)
                                                                                                                                  aaaGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTGGTATCTGCAATTCTTTTTCTATGCCTGGGGCCACGCCCGCATTCCGGCGCAGTATGACTACATCAGTTGGAtgctgc                 <  8:397972/151‑1 (MQ=255)
                                                                                                                                  aaaGCCGACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTGGTATCTGCAATTCTTTTTCTATGCCTGGGGCCACGCCCGCATTCCGGCGCAGTATGACTACATCAGTTGGAtgctgc                 >  7:1381944/1‑151 (MQ=255)
                                                                                                                                      ccGACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTGGTATCTGCAATTCTTTTTCTATGCCTGGGGCCACGCCCGCATTCCGGCGCAGTATGACTACATCAGTTGGATGCTGCatat             <  3:177838/151‑1 (MQ=255)
                                                                                                                                       cGACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTGGTATCTGCAATTCTTTTTCTATGCCTGGGGCCACGCCCGCATTCCGGCGCAGTATGACTACATCAGTTGGATGCTGCATATg            <  8:703870/151‑1 (MQ=255)
                                                                                                                                        gACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTGGTATCTGCAATTCTTTTTCTATGCCTGGGGCCACGCCCGCATTCCGGCGCAGTATGACTACATCAGTTGGATGCTGCATATGa           <  8:868793/151‑1 (MQ=255)
                                                                                                                                        gACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTGGTATCTGCAATTCTTTTTCTATGCCTGGGGCCACGCCCGCATTCCGGCGCAGTATGACTACATCAGTTGGATGCTGCATATGa           <  5:994717/151‑1 (MQ=255)
                                                                                                                                        gACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTGGTATCTGCAATTCTTTTTCTATGCCTGGGGCCACGCCCGCATTCCGGCGCAGTATGACTACATCAGTTGGATGCTGCATATGa           <  5:224017/151‑1 (MQ=255)
                                                                                                                                        gACTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTGGTATCTGCAATTCTTTTTCTATGCCTGGGGCCACGCACGAATTCCGGCGAAGTATGACTACATCAGTTGGATGCTGAATATg            >  8:1558047/1‑150 (MQ=255)
                                                                                                                                         aCTTCTCGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTGGTATCTGCAATTCTTTTTCTATGCCTGGGGCCACGCCCGCATTCCGGCGCAGTATGACTACATCAGTTGGATGCTGCATATGAg          <  2:1814809/151‑1 (MQ=255)
                                                                                                                                              tcGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTGGTATCTGCAATTCTTTTTCTATGCCTGGGGCCACGCCCGCATTCCGGCGCAGTATGACTACATCAGTTGGATGCTGCATATGAGTTTCt     >  3:616617/1‑151 (MQ=255)
                                                                                                                                               cGCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTGGTATCTGCAATTCTTTTTCTATGCCTGGGGCCACGCCCGCATTCCGGCGCAGTATGACTACATCAGTTGGATGCTGCATATGAGttt       <  1:1259113/148‑1 (MQ=255)
                                                                                                                                                gCTGACAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTGGTATCTGCAATTCTTTTTCTATGCCTGGGGCCACGCCCGCATTCCGGCGCAGTATGACTACATCAGTTGGATGCTGCATATGAGTTTCTAt   <  4:1598782/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CGCTGCCGCACTTGGCGTCGATCCACTGTATGTCGCTCTGCCAAGCTATGTTGTCATCATGGGCGGCGGCGCGATCATTAACCTCGGTTTCTGTTTTATTCGTCTGGCAAAAGTGAAGGATTTGTCGCTAAAAGCCGACTTCTCGCTGGCAAAATCGCTGATCATTCACAATGTGTTACTCTCGACACTGGGCGGGTTGATGTGGTATCTGCAATTCTTTTTCTATGCCTGGGGCCACGCCCGCATTCCGGCGCAGTATGACTACATCAGTTGGATGCTGCATATGAGTTTCTATG  >  DH1/4414547‑4414842

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑