Predicted mutation
evidence seq id position mutation annotation gene description
RA DH1 4,482,593 C→A E239D (GAG→GAT)  pepQ ← Xaa‑Pro dipeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH14,482,5930CA98.7% 2262.0 / 10.4 602E239D (GAG→GAT) pepQXaa‑Pro dipeptidase
Reads supporting (aligned to +/- strand):  ref base C (4/4);  new base A (267/327);  total (271/331)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

Only 100 of 614 total aligned reads displayed.

TCATGGTGGCGATCAGCGCCAGTTGTTCATCATTAACGTCCTTCACCAGCTGCGCGTAGTCGTTGTCACTTTTTGCCGACCAGGTACGGGTCAGGTCAGCCGCATAGCCGTTATATTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATCTCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTACCGCAGCGTGTTCGTTAAGCGCCACAATGTTGCTGTAAGGTACGTCGGTATCACGATGACCGGTCGCGGTCAGATAGGCAATGTTGATATCGAACTCGCTCATGCCA  >  DH1/4482445‑4482740
                                                                                                                                                    |                                                                                                                                                   
 cATGGTGGCGATCAGCGCCAGTTGTTCATCATTAACGTCCTTCACCAGCTGCGCGTAGTCGTTGTCACTTTTTGCCGACCAGGTACGGGTCAGGTCAGCCGCATAGCCGTTATATTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATctct                                                                                                                                                  <  4:1272015/151‑1 (MQ=255)
 cATGGTGGCGATCAGCGCCAGTTGTTCATCATTAACGTCCTTCACCAGCTGCGCGTAGTCGTTGTCACTTTTTGCCGACCAGGTACGGGTCAGGTCAGCCGCATAGCCGTTATATTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATatct                                                                                                                                                  <  6:1724732/151‑1 (MQ=255)
  aTGGTGGCGATCAGCGCCAGTTGTTCATCATTAACGTCCTTCACCAGCTGCGCGTAGTCGTTGTCACTTTTTGCCGACCAGGTACGGGTCAGGTCAGCCGCATAGCCGTTATATTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATATCtt                                                                                                                                                 <  6:1196851/151‑1 (MQ=255)
    ggtggCGATCAGCGCCAGTTGTTCATCATTAACGTCCTTCACCAGCTGCGCGTAGTCGTTGTCACTTTTTGCCGACCAGGTACGGGTCAGGTCAGCCGCATAGCCGTTATATTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATATCTTcc                                                                                                                                               <  3:1611485/151‑1 (MQ=255)
       ggCGATCAGCGCCAGTTGTTCATCATTAACGTCCTTCACCAGCTGCGCGTAGTCGTTGTCACTTTTTGCCGACCAGGTACGGGTCAGGTCAGCCGCATAGCCGTTATATTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATATCTTCCGGt                                                                                                                                            <  8:1248045/151‑1 (MQ=255)
       ggCGATCAGCGCCAGTTGTTCATCATTAACGACCTTCACCAGCTGCGCGTAGTCGTTGTCACTTTTTGCCGACCAGGTACGGGTCAGGTCAGCCGCATAGCCGTTATATTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATATCTTCCGGt                                                                                                                                            <  5:630706/151‑1 (MQ=255)
        gCGATCAGCGCCAGTTGTTCATCATTAACGTCCTTCACCAGCTGCGCGTAGTCGTTGTCACTTTTTGCCGACCAGGTACGGGTCAGGTCAGCCGCATAGCCGTTATATTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATATCTTCCGGTg                                                                                                                                           <  1:1767467/151‑1 (MQ=255)
         cGATCAGCGCCAGTTGTTCATCATTAACGTCCTTCACCAGCTGCGCGTAGTCGTTGTCACTTTTTGCCGACCAGGTACGGGTCAGGTCAGCCGCATAGCCGTTATATTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATATCTTCCGGTGc                                                                                                                                          <  2:366464/151‑1 (MQ=255)
               gcgcCAGTTGTTCATCATTAACGTCCTTCACCAGCTGCGCGTAGTCGTTGTCACTTTTTGCCGACCAGGTACGGGTCAGGTCAGCCGCATAGCCGTTATATTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATATCTTCCGGTGCCTGATg                                                                                                                                    >  7:444485/1‑151 (MQ=255)
                 gcCAGTTGTTCATCATTAACGTCCTTCACCAGCTGCGCGTAGTCGTTGTCACTTTTTGCCGACCAGGTACGGGTCAGGTCAGCCGCATAGCCGTTATATTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATATCTTCCGGTGCCTGATGGt                                                                                                                                  >  6:1834191/1‑151 (MQ=255)
                    aGTTGTTCATCATTAACGTCCTTCACCAGCTGCGCGTAGTCGTTGTCACTTTTTGCCGACCAGGTACGGGTCAGGTCAGCCGCATAGCCGTTATATTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCa                                                                                                                               <  6:737586/151‑1 (MQ=255)
                    aGTTGTTCATCATTAACGTCCTTCACCAGCTGCGCGTAGTCGTTGTCACTTTTTGCCGACCAGGTACGGGTCAGGTCAGCCGCATAGCCGTTATATTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCa                                                                                                                               <  7:1639410/151‑1 (MQ=255)
                        gttCATCATTAACGTCCTTCACCAGCTGCGCGTAGTCGTTGTCACTTTTTGCCGACCAGGTACGGGTCAGGTCAGCCGCATAGCCGTTATATTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGtt                                                                                                                            <  8:1652834/150‑1 (MQ=255)
                            atcatTAACGTCCTTCACCAGCTGCGCGTAGTCGTTGTCACTTTTTGCCGACCAGGTACGGGTCAGGTCAGCCGCATAGCCGTTATATTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGgtg                                                                                                                       <  7:257835/151‑1 (MQ=255)
                             tcatTAACGTCCTTCACCAGCTGCGCGTAGTCGTTGTCACTTTTTGCCGACCAGGTACGGGTCAGGTCAGCCGCATAGCCGTTATATTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGgtgt                                                                                                                      >  2:1482164/1‑151 (MQ=255)
                                 tAACGTCCTTCACCAGCTGCGCGTAGTCGTTGTCACTTTTTGCCGACCAGGTACGGGTCAGGTCAGCCGCATAGCCGTTATATTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAAt                                                                                                                   <  1:1843439/150‑1 (MQ=255)
                                 tAACGTCCTTCACCAGCTGCGCGTAGTCGTTGTCACTTTTTGCCGACCAGGTACGGGTCAGGTCAGCCGCATAGCCGTTATATTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATg                                                                                                                  >  4:1656853/1‑151 (MQ=255)
                                  aaCGTCCTTCACCAGCTGCGCGTAGTCGTTGTCACTTTTTGCCGACCAGGTACGGGTCAGGTCAGCCGCATAGCCGTTATATTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGc                                                                                                                 <  5:393052/151‑1 (MQ=255)
                                    cGTCCTTCACCAGCTGCGCGTAGTCGTTGTCACTTTTTGCCGACCAGGTACGGGTCAGGTCAGCCGCATAGCCGTTATATTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAg                                                                                                               <  3:1290111/151‑1 (MQ=255)
                                      tCCTTCACCAGCTGCGCGTAGTCGTTGTCACTTTTTGCCGACCAGGTACGGGTCAGGTCAGCCGCATAGCCGTTATATTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGt                                                                                                              >  7:638223/1‑150 (MQ=255)
                                       ccTTCACCAGCTGCGCGTAGTCGTTGTCACTTTTTGCCGACCAGGTACGGGTCAGGTCAGCCGCATAGCCGTTATATTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTAc                                                                                                            >  1:1882498/1‑151 (MQ=255)
                                       ccTTCACCAGCTGCGCGTAGTCGTTGTCACTTTTTGCCGACCAGGTACGGGTCAGGTCAGCCGCATAGCCGTTATATTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTAc                                                                                                            <  6:570979/151‑1 (MQ=255)
                                         ttCACCAGCTGCGCGTAGTCGTTGTCACTTTTTGCCGACCAGGTACGGGTCAGGTCAGCCGCATAGCCGTTATATTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTACCg                                                                                                          >  1:629075/1‑151 (MQ=255)
                                          tCACCAGCTGCGCGTAGTCGTTGTCACTTTTTGCCGACCAGGTACGGGTCAGGGCAGCCGCATAGCCGTTATATTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTAcc                                                                                                           <  4:1474880/149‑1 (MQ=255)
                                           cACCAGCTGCGCGTAGTCGTTGTCACTTTTTGCCGACCAGGTACGGGTCAGGTCAGCCGCATAGCCGTTATATTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTACCGCa                                                                                                        <  3:196436/151‑1 (MQ=255)
                                            aCCAGCTGCGCGTAGTCGTTGTCACTTTTTGCCGACCAGGTACGGGTCAGGTCAGCCGCATAGCCGTTATATTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTACCGc                                                                                                         >  2:1324076/1‑149 (MQ=255)
                                               aGCTGCGCGTAGTCGTTGTCACTTTTTGCCGACCAGGTACGGGTCAGGTCAGCCGCATAGCCGTTATATTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTACCGCAGCgt                                                                                                    <  6:1928193/151‑1 (MQ=255)
                                               aGCTGCGCGTAGTCGTTGTCACTTTTTGCCGACCAGGTACGGGTCAGGTCAGCCGCATAGCCGTTATATTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTACCGCAGCgg                                                                                                    >  8:1840292/1‑150 (MQ=255)
                                                   gcgcGTAGTCGTTGTCACTTTTTGCCGACCAGGTACGGGTCAGGTCAGCCGCATAGCCGTTATATTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTACCGCAGCgtgt                                                                                                  <  8:241136/149‑1 (MQ=255)
                                                    cgcgTAGTCGTTGTCACTTTTTGCCGACCAGGTACGGGTCAGGTCAGCCGCATAGCCGTTATATTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTACCGCAGCGTGTTCg                                                                                               >  1:142208/1‑151 (MQ=255)
                                                      cgTAGTCGTTGTCACTTTTTGCCGACCAGGTACGGGTCAGGTCAGCCGCATAGCCGTTATATTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTACCGCAGCGTGTTCGtt                                                                                             >  4:541207/1‑151 (MQ=255)
                                                       gTAGTCGTTGTCACTTTTTGCCGACCAGGTACGGGTCAGGTCAGCCGCATAGCCGTTATATTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTACCGCAGCGTGTTCGtt                                                                                             >  5:1704221/1‑150 (MQ=255)
                                                       gTAGTCGTTGTCACTTTTTGCCGACCAGGTACGGGTCAGGTCAGCCGCATAGCCGTTATATTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTACCGCAGCGTGTTCGtt                                                                                             >  2:1740607/1‑150 (MQ=255)
                                                       gTAGTCGTTGTCACTTTTTGCCGACCAGGTACGGGTCAGGTCAGCCGCATAGCCGTTATATTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTACCGCAGCGTGTTCGTTa                                                                                            <  7:801342/151‑1 (MQ=255)
                                                         aGTCGTTGTCACTTTTTGCCGACCAGGTACGGGTCAGGTCAGCCGCATAGCCGTTATATTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTACCGCAGCGTGTTCGTTaa                                                                                           <  7:1149596/150‑1 (MQ=255)
                                                          gTCGTTGTCACTTTTTGCCGACCAGGTACGGGTCAGGTCAGCCGCATAGCCGTTATATTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTACCGCAGCGTGTTCGTTAAgc                                                                                         <  8:342807/151‑1 (MQ=255)
                                                            cGTTGTCACTTTTTGCCGACCAGGTACGGGTCAGGTCAGCCGCATAGCCGTTATATTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTACCGCAGCGTGTTCGTTAAgcg                                                                                        <  4:29572/150‑1 (MQ=255)
                                                               tGTCACTTTTTGCCGACCAGGTACGGGTCAGGTCAGCCGCATAGCCGTTATATTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTACCGCAGCGTGTTCGTTAAGCGCca                                                                                     <  2:695804/150‑1 (MQ=255)
                                                                gTCACTTTTTGCCGACCAGGTACGGGTCAGGTCAGCCGCATAGCCGTTATATTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTACCGCAGCGTGTTCGTTAAGCGCcac                                                                                    >  4:1108109/1‑150 (MQ=255)
                                                                  cACTTTTTGCCGACCAGGTACGGGTCAGGTCAGCCGCATAGCCGTTATATTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTACCGCAGCGTGTTCGTTAAGCGCCACAAt                                                                                 >  7:1789313/1‑151 (MQ=255)
                                                                  cACTTTTTGCCGACCAGGTACGGGTCAGGTCAGCCGCATAGCCGTTATATTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTACCGCAGCGTGTTCGTTAAGCGCCACAAt                                                                                 <  7:12778/151‑1 (MQ=255)
                                                                    cTTTTTGCCGACAAGGTACGGGTCAGGTCAGCCGCATAGCCGATATATTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATAACTTCCGGTGCCTGATGGTCCACTTAGGTGTAATGCAGTACCGCAGCGTGTTCGTTAAGCGCCACAATGt                                                                               >  5:1317735/1‑151 (MQ=255)
                                                                      ttttGCCGACCAGGTACGGGTCAGGTCAGCCGCATAGCCGTTATATTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTACCGCAGCGTGTTCGTTAAGCGCCACAATGt                                                                               <  2:79223/149‑1 (MQ=255)
                                                                         tGCCGACCAGGTACGGGTCAGGTCAGCCGCATAGCCGTTATATTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTACCGCAGCGTGTTCGTTAAGCGCCACAATGTTGCt                                                                           <  6:42810/150‑1 (MQ=255)
                                                                         tGCCGACCAGGTACGGGTCAGGTCAGCCGCATAGCCGTTATATTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTACCGCAGCGTGTTCGTTAAGCGCCACAATGTTGCTg                                                                          >  7:966084/1‑151 (MQ=255)
                                                                          gCCGACCAGGTACGGGTCAGGTCAGCCGCATAGCCGTTATATTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTACCGCAGCGTGTTCGTTAAGCGCCACAATGTTGCTGt                                                                         >  3:1006787/1‑151 (MQ=255)
                                                                          gCCGACCAGGTACGGGTCAGGTCAGCCGCATAGCCGTTATATTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTACCGCAGCGTGTTCGTTAAGCGCCACAATGTTGCTGt                                                                         >  1:869513/1‑151 (MQ=255)
                                                                            cGACCAGGTACGCGTCAGGTCAGCCGCATAGCCGTTATATTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATATCTTCCGGTGCCTGATGCTCCAGTTAGGTGTAATGCAGTACCTCAGCGTGTTCTTTAATCGAAACAATGTTGCTCTa                                                                        >  8:1963592/1‑150 (MQ=255)
                                                                              aCCAGGTACGGGTCAGGTCAGCCGCATAGCCGTTATATTCTGACCCGGCATCCAGCAGGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTACCGCAGCGTGTTCGTTAAGCGCCACAATGTTGCTGTAAgg                                                                     >  5:1955993/1‑151 (MQ=255)
                                                                               ccAGGTACGGGTCAGGTCAGCCGCATAGCCGTTATATTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTACCGCAGCGTGTTCGTTAAGCGCCACAATGTTGCTGTAAGGt                                                                    <  1:701732/151‑1 (MQ=255)
                                                                                  ggTACGGGTCAGGTCAGCCGCATAGCCGTTATATTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTACCGCAGCGTGTTCGTTAAGCGCCACAATGTTGCTGTAAGGTACg                                                                 >  5:155374/1‑151 (MQ=255)
                                                                                   gTACGGGTCAGGTCAGCCGCATAGCCGTTATATTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTACCGCAGCGTGTTCGTTAAGCGCCACAATGTTGCTGTAAGGTACGt                                                                <  8:782301/151‑1 (MQ=255)
                                                                                     aCGGGTCAGGTCAGCCGCATAGCCGTTATATTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTACCGCAGCGTGTTCGTTAAGCGCCACAATGTTGCTGTAAGGTACGTc                                                               <  4:40431/150‑1 (MQ=255)
                                                                                     aCGGGTCAGGTCAGCCGCATAGCCGTTATATTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTACCGCAGCGTGTTCGTTAAGCGCCACAATGTTGCTGTAAGGTACGTc                                                               <  3:695697/150‑1 (MQ=255)
                                                                                          gcaggtcagCCGCATAGCCGTTATATTCGTCCCCGGCATCCAGCAGGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTACCGCAGCGTGTTCGTTAAGCGCCACAATGTTGCTGTAAGGTACGTCGGTATc                                                         <  8:477381/150‑1 (MQ=255)
                                                                                           caggtcagCCGCATAGCCGTTATATTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTACCGCAGCGTGTTCGTTAAGCGCCACAATGTTGCTGTAAGGTACGTCGGTATc                                                         >  3:1719430/1‑150 (MQ=255)
                                                                                           caggtcagCCGCATAGCCGTTATATTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTACCGCAGCGTGTTCGTTAAGCGCCACAATGTTGCTGTAAGGTACGTCGGTATCa                                                        <  6:54154/151‑1 (MQ=255)
                                                                                           caggtcagCCGCATAGCCGTTATATTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTACCGCAGCGTGTTCGTTAAGCGCCACAATGTTGCTGTAAGGTACGTCGGTATCa                                                        <  2:1590974/151‑1 (MQ=255)
                                                                                           caggtcagCCGCATAGCCGTTATATTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTACCGCAGCGTGTTCGTTAAGCGCCACAATGTTGCTGTAAGGTACGTCGGTATCa                                                        <  3:1842038/151‑1 (MQ=255)
                                                                                           caggtcagCCGCATAGCAGTTATATTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTACCGCAGCGTGTTCGTTAAGCGCCACAATGTTGCTGTAAGGTACGTCGGTATc                                                         <  5:1177502/150‑1 (MQ=255)
                                                                                            aggtcagCCGCATAGCCGTTATATTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTACCGCAGCGTGTTCGTTAAGCGCCACAATGTTGCTGTAAGGTACGTCGGTATCAc                                                       <  6:974689/151‑1 (MQ=255)
                                                                                            aggtcagCCGCATAGCCGTTATATTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATATCTTACGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTACAGCAGCGTGTTCGTTAAGCGCCACAATGTTGCTGTAAGCTACGTCGGTATCAc                                                       >  7:1889959/1‑151 (MQ=255)
                                                                                                cagCCGCATAGCCGTTATATTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTACCGCAGCGTGTTCGTTAAGCGCCACAATGTTGCTGTAAGGTACGTCGGTATCACGATg                                                   >  5:557525/1‑151 (MQ=255)
                                                                                                cagCCGCATAGCCGTTATATTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTACCGCAGCGTGTTCGTTAAGCGCCACAATGTTGCTGTAAGGTACGTCGGTATCACGATg                                                   >  6:1313675/1‑151 (MQ=255)
                                                                                                    cGCATAGCCGTTATATTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTCGTGTAATGCAGTACCGCAGCGTGATCGTAAAGCGCCACAATCTTCCTCTAAGGAACGTCGCTATCACCATGACCg                                               >  6:285614/1‑151 (MQ=255)
                                                                                                     gCATAGCCGTTATATTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTACCGCAGCGTGTTCGTTAAGCGCCACAATGTTGCTGTAAGGTACGTCGGTATCACGATGACCgg                                              <  3:536618/151‑1 (MQ=255)
                                                                                                      cATAGCCGTTATATTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTACCGCAGCGTGTTCGTTAAGCGCCACAATGTTGCTGTAAGGTACGTCGGTATCACGATGACCGGt                                             >  1:1647329/1‑151 (MQ=255)
                                                                                                      cATAGCCGTTATATTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTACCGCAGCGTGTTCGTTAAGCGCCACAATGTTGCTGTAAGGTACGTCGGTATCACGATGACCGGt                                             <  3:94879/151‑1 (MQ=255)
                                                                                                       aTAGCCGTTATATTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTACCGCAGCGTGTTCGTTAAGCGCCACAATGTTGCTGTAAGGTACGTCGGTATCACGATGACCGGTc                                            <  2:1388773/151‑1 (MQ=255)
                                                                                                          gCCGTTATATTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTACCGCAGCGTGTTCGTTAAGCGCCACAATGTTGCTGTAAGGTACGTCGGTATCACGATGACCGGTcgcg                                         >  4:652347/1‑151 (MQ=255)
                                                                                                           ccGTTATATTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTACCGCAGCGTGTTCGTTAAGCGCCACAATGTTGCTGTAAGGTACGTCGGTATCACGATGACCGGTCGCgg                                        <  1:569464/151‑1 (MQ=255)
                                                                                                             gTTATATTCTGCCCCGGCATCCAGGAGGAAGCTGCGCATATCTTCGGGTGCCTGATGGTCGAGTTTGGTGTAATGCAGTACCGCAGCGTGTTCGTTAAGCGCCAGAATGTTGCTGTAAGGTACGTCGGTATCACGATGACGGGTCGCGGTc                                      <  2:1048937/151‑1 (MQ=255)
                                                                                                              ttATATTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTACCGCAGCGTGTTCGTTAAGCGCCACAATGTTGCTGTAAGGTACGTCGGTATCACGATGACCGGTCGCGGTc                                      >  4:200040/1‑150 (MQ=255)
                                                                                                               tataTTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTACCGCAGCGTGTTCGTTAAGCGCCACAATGTTGCTGTAAGGTACGTCGGTATCACGATGACCGGTCGCGGTc                                      >  6:1123200/1‑149 (MQ=255)
                                                                                                               tataTTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTACCGCAGCGTGTTCGTTAAGCGCCACAATGTTGCTGTAAGGTACGTCGGTATCACGATGACCGGTCGCGGTCa                                     <  2:578209/150‑1 (MQ=255)
                                                                                                                      tGCCCCGGCATCCAGCAGGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTACCGCAGCGTGTTCGTTAAGCGCCACAATGTTGCTGTAAGGTACGTCGGTATCACGATGACCGGTCGCGGTCAGATAGGc                              >  6:1419860/1‑150 (MQ=255)
                                                                                                                       gCCCCGGCATCCAGCAGGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTACCGCAGCGTGTTCGTTAAGCGCCACAATGTTGCTGTAAGGTACGTCGGTATCACGATGACCGGTCGCGGTCAGATAgg                               <  3:38418/148‑1 (MQ=255)
                                                                                                                       gCCCCGGCATCCAGCAGGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTACCGCAGCGTGTTCGTTAAGCGCCACAATGTTGCTGTAAGGTACGTCGGTATCACGATGACCGGTCGCGGTCAGATAGGCa                             <  5:1447481/150‑1 (MQ=255)
                                                                                                                       gCCCCGGCATCCAGCAGGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTACCGCAGCGTGTTCGTTAAGCGCCACAATGTTGCTGTAAGGTACGTCGGTATCACGATGACCGGTCGCGGTCAGATAGGCa                             >  7:143969/1‑150 (MQ=255)
                                                                                                                          ccGGCATCCAGCAGGAAGGTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTACCGCAGCGTGTTCGTTAAGCGCCACAATGTTGCTGTAAGGTACGTCGGTATCACGATGACCGGTCGCGGGCAGATAGGCAATGt                         <  1:1374005/151‑1 (MQ=255)
                                                                                                                             gCATCCAGCAGGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTACCGCAGCGTGTTCGTTAAGCGCCACAATGTTGCTGTAAGGTACGTCGGTATCACGATGACCGGTCGCGGTCAGATAGGCAATGTTg                       >  1:1006478/1‑150 (MQ=255)
                                                                                                                              cATCCAGCAGGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTACCGCAGCGTGTTCGTTAAGCGCCACAATGTTGCTGTAAGGTACGTCGGTATCACGATGACCGGTCGCGGTCAGATAGGCAATGTTGat                     <  8:1572573/151‑1 (MQ=255)
                                                                                                                               aTCCAGCAGGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTACCGCAGCGTGTTCGTTAAGCGCCACAATGTTGCTGTAAGGTACGTCGGTATCACGATGACCGGTCGCGGTCAGATAGGCAATGTTGat                     >  7:741686/1‑150 (MQ=255)
                                                                                                                               aTCCAGCAGGAAGCTGCGCATATCTTCCGGTGCATGATGGTCCAGTTTGGTGTAATGCAGTACCGCAGCGTGTTCGTTAAGCGCCACAATGTTGCTGTAAGGTACGTCGTTATCACGATGACCGGTAGCTGTAAGATAGGCAATGTTgct                     >  6:1660409/1‑148 (MQ=255)
                                                                                                                                 ccAGCAGTAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTACCGCAGCGTGTTCGTTAAGCGCCACAATGTTGCTGTAAGGTACGTCGGTATCACGATGACCGGTCGCGGTCAGATAGGCAATGTTGATATc                  <  1:1209896/151‑1 (MQ=255)
                                                                                                                                 ccAGCAGGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTACCGCAGCGTGTTCGTTAAGCGCCACAATGTTGCTGTAAGGTACGTCGGTATCACGATGACCGGTCGCGGTCAGATAGGCAATGTTGATATc                  <  3:1127493/151‑1 (MQ=255)
                                                                                                                                  cagcagGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTACCGCAGCGTGTTCGTTAAGCGCCACAATGTTGCTGTAAGGTACGTCGGTATCACGATGACCGGTCGCGGTCAGATAGGCAATGTTGATATCg                 <  6:1556718/151‑1 (MQ=255)
                                                                                                                                  cagcagGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTACCGCAGCGTGTTCGTTAAGCGCCACAATGTTGCTGTAAGGTACGTCGGTATCACGATGACCGGTCGCGGTCAGATAGGCAATGTTGATATCg                 <  5:1843825/151‑1 (MQ=255)
                                                                                                                                  cagcagGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTACCGCAGCGTGTTCGTTAAGCGCCACAATGTTGCTGTAAGGTACGTCGGTATCACGATGACCGGTCGCGGTCAGATAGGCAATGTTGATATCg                 <  2:122677/151‑1 (MQ=255)
                                                                                                                                      agGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTACCGCAGCGTGTTCGTTAAGCGCCACAATGTTGCTGTAAGGTACGTCGGTATCACGATGACCGGTCGCGGTCAGATAGGCAATGTTGATATCGAACt             <  4:90830/151‑1 (MQ=255)
                                                                                                                                      agGAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTACCGCAGCGTGTTCGTTAAGCGCCACAATGTTGCTGTAAGGTACGTCGGTATCACGATGACCGGTCGCGGTCAGATAGGCAATGTTGATATCGAACt             <  3:800574/151‑1 (MQ=255)
                                                                                                                                       ggAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTACCGCAGCGTGTTCGTTAAGCGCCACAATGTTGCTGTAAGGTACGTCGGTATCACGATGACCGGTCGCGGTCAGATAGGCAATGTTGATATCGAACTc            <  1:459914/151‑1 (MQ=255)
                                                                                                                                        gAAGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTACCGCAGCGTGTTCGTTAAGCGCCACAATGTTGCTGTAAGGTACGTCGGTATCACGATGACCGGTCGCGGTCAGATAGGCAATGTTGATATCGAACTCg           >  2:20756/1‑151 (MQ=255)
                                                                                                                                         aaGCTGCGCATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTACCGCAGCGTGTTCGTTAAGCGCCACAATGTTGCTGTAAGGTACGTCGGTATCACGATGACCGGTCGCGGTCAGATAGGCAATGTTGATATCGAACTCGc          >  8:1126964/1‑151 (MQ=255)
                                                                                                                                          aGCTGCGCATATCTTCCGGTGCCTGATGGTCCATTTTGGTGTAATGCAGTACCGCAGCGTGTTCGTTAAGCGCCACAATGTTGCTGTAAGGTACGTCGGTATCACGATGACCGGTCGCGGTCAGATAGGCAATGTTGATATCGAACTCGCt         <  5:975864/151‑1 (MQ=255)
                                                                                                                                              gcgcATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTACCGCAGCGTGTTCGTTAAGCGCCACAATGTTGCTGTAAGGTACGTCGGTATCACGATGACCGGTCGCGGTCAGATAGGCAATGTTGATATCGAACTCGCTCAt      <  2:1801593/150‑1 (MQ=255)
                                                                                                                                              gcgcATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTACCGCAGCGTGTTCGTTAAGCGCCACAATGTTGCTGTAAGGTACGTCGGTATCACGATGACCGGTCGCGGTCAGATAGGCAATGTTGATATCGAACTCGCTCATg     >  5:1113356/1‑151 (MQ=255)
                                                                                                                                               cgcATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTACCGCAGCGTGTTCGTTAAGCGCCACAATGTTGCTGTAAGGTACGTCGGTATCACGATGACCGGACGCGGTCAGATAGGCAATGTTGATATCGAACTCGCTCATGc    <  8:656606/151‑1 (MQ=255)
                                                                                                                                                gcATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTACCGCAGCGTGTTCGTTAAGCGCCACAATGTTGCTGTAAGGTACGTCGGTATCACGATGACCGGTCGCGGTCAGATAGGCAATGTTGATATAGAACTCGCTCATGcc   >  4:1894428/1‑151 (MQ=255)
                                                                                                                                                 cATATCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTACCGCAGCGTGTTCGTTAAGCGCCACAATGTTGCTGTAAGGTACGTCGGTATCACGATGACCGGTCGCGGTCAGATAGGCAATGTTGATATCGAACTCGCTCATGCCa  <  1:199478/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TCATGGTGGCGATCAGCGCCAGTTGTTCATCATTAACGTCCTTCACCAGCTGCGCGTAGTCGTTGTCACTTTTTGCCGACCAGGTACGGGTCAGGTCAGCCGCATAGCCGTTATATTCTGCCCCGGCATCCAGCAGGAAGCTGCGCATCTCTTCCGGTGCCTGATGGTCCAGTTTGGTGTAATGCAGTACCGCAGCGTGTTCGTTAAGCGCCACAATGTTGCTGTAAGGTACGTCGGTATCACGATGACCGGTCGCGGTCAGATAGGCAATGTTGATATCGAACTCGCTCATGCCA  >  DH1/4482445‑4482740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑