Predicted mutation
evidence seq id position mutation annotation gene description
RA DH1 1,493,381 T→C L292L (TTA→TTG)  elaD ← deubiquitinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH11,493,3810TC98.5% 1943.9 / 6.2 521L292L (TTA→TTG) elaDdeubiquitinase
Reads supporting (aligned to +/- strand):  ref base T (2/6);  new base C (232/279);  total (235/286)
Fisher's exact test for biased strand distribution p-value = 3.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 526 total aligned reads displayed.

GGTTTTTTAATAACGTATAGGGGGCGCTGTCAGAATTGCTTTCCCTTTGTTCAATCACTTCCTGGGCTGCCATGCACACAAATGCACCGCAACTTTGGCTTAAGTATTGCTGAAGATCGGCATGTAAAAATATTATCTCTGGTTCATTTAAATGAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTCAGAATTAAAACTATTAATGACACGCTTAAGTGAATTTTCTTTATCATAGCCTAGTGCACGCAATGAGTTGAATATGACGCATTTTATTTGTAGTTTATTATTT  >  DH1/1493233‑1493528
                                                                                                                                                    |                                                                                                                                                   
   tttttAATAACGTATAGGGGGCGCTGTCAGAATTGCTTTCCCTTTGTTCAATCACTTCCTGGGCTGCCATGCACACAAATGCACCGCAACTTTGGCTTAAGTATTGCTGAAGATCGGCATGTAAAAATATTATCTCTGGTTCATTCAAAt                                                                                                                                                 <  6:1130928/150‑1 (MQ=255)
    ttttAATAACGTATAGGGGGCGCTGTCAGAATTGCTTTCCCTTTGTTCAATCACTTCCTGGGCTGCCATGCACACAAATGCACCGCAACTTTGGCTTAAGTATTGCTGAAGATCGGCATGTAAAAATATTATCTCTGGTTCATTCAAATg                                                                                                                                                <  3:1145040/150‑1 (MQ=255)
    ttttAATAACGTATAGGGGGCGCTGTCAGAATTGCTTTCCCTTTGTTCAATCACTTCCTGGGCTGCCATGCACACAAATGCACCGCAACTTTGGCTTAAGTATTGCTGAAGATCGGCATGTAAAAATATTATCTCTGGTTCATTCAAATg                                                                                                                                                <  1:212317/150‑1 (MQ=255)
     tttAATAACGTATAGGGGGCGCTGTCAGAATTGCTTTCCCTTTGTTCAATCACTTCCTGGGCTGCCATGCACACAAATGCACCGCAACTTTGGCTTAAGTATTGCTGAAGATCGGCATGTAAAAATATTATCTCTGGTTCATTCAAATGa                                                                                                                                               <  6:1277875/150‑1 (MQ=255)
     tttAATAACGTATAGGGGGCGCTGTCAGAATTGCTTTCCCTTTGTTCAATCACTTCCTGGGCTGCCATGCACACAAATGCACCGCAACTTTGGCTTAAGTATTGCTGAAGATCGGCATGTAAAAATATTATCTCTGGTTCATTCAAATGa                                                                                                                                               >  6:1053290/1‑150 (MQ=255)
      ttAATAACGTATAGGGGGCGCTGTCAGAATTGCTTTCCCTTTGTTCAATCACTTCCTGGGCTGCCATGCACACAAATGCACCGCAACTTTGGCTTAAGTATTGCTGAAGATCGGCATGTAAAAATATTATCTCTGGTTCATTCAAATGaa                                                                                                                                              <  5:743462/150‑1 (MQ=255)
      ttAATAACGTATAGGGGGCGCTGTCAGAATTGCTTTCCCTTTGTTCAATCACTTCCTGGGCTGCCATGCACACAAATGCACCGCAACTTTGGCTTAAGTATTGCTGAAGATCGGCATGTAAAAATATTATCTCTGGTTCATTCAAATGaa                                                                                                                                              <  7:181357/150‑1 (MQ=255)
       taataaCGTATAGGGGGCGCTGTCAGAATTGCTTTCCCTTTGTTCAATCACTTCCTGGGCTGCCATGCACACAAATGCACCGCAACTTTGGCTTAAGTATTGCTGAAGATCGGCATGTAAAAATATTATCTCTGGTTCATTCAAATGAACt                                                                                                                                            >  4:1023050/1‑151 (MQ=255)
         ataaCGTATAGGGGGCGCTGTCAGAATTGCTTTCCCTTTGTTCAATCACTTCCTGGGCTGCCATGCACACAAATGCACCGCAACTTTGGCTTAAGTATTGCTGAAGATCGGCATGTAAAAATATTATCTCTGGTTCATTCAAATGAACttt                                                                                                                                          >  1:1938952/1‑151 (MQ=255)
             cGTATAGGGGGCGCTGTCAGAATTGCTTTCCCTTTGTTCAATCACTTCCTGGGCTGCCATGCACACAAATGCACCGCAACTTTGGCTTAAGTATTGCTGAAGATCGGCATGTAAAAATATTATCTCTGGTTCATTCAAATGAACTTTtat                                                                                                                                       >  8:1441424/1‑150 (MQ=255)
                ataGGGGGCGCTGTCAGAATTGCTTTCCCTTTGTTCAATCACTTCCTGGGCTGCCATGCACACAAATGCACCGCAACTTTGGCTTAAGTATTGCTGAAGATCGGCATGTAAAAATATTATCTCTGGTTCATTCAAATGAACTTTTAtattt                                                                                                                                   >  6:590618/1‑150 (MQ=255)
                ataGGGGGCGCTGTCAGAATTGCTTTCCCTTTGTTCAATCACTTCCTGGGCTGCCATGCACACAAATGCACCGCAACTTTGGCTTAAGTATTGCTGAAGATCGGCATGTAAAAATATTATCTCTGGTTCATTCAAATGAACTTTTAtatta                                                                                                                                   <  6:1975950/151‑1 (MQ=255)
                  aGGGGGCGCTGTCAGAATTGCTTTCCCTTTGTTCAATCACTTCCTGGGCTGCCATGCACACAAATGCACCGCAACTTTGGCTTAAGTATTGCTGAAGATCGGCATGTAAAAATATTATCTCTGGTTCATTCAAATGAACTTTTAtattat                                                                                                                                  <  1:592390/150‑1 (MQ=255)
                    ggggCGCTGTCAGAATTGCTTTCCCTTTGTTCAATCACTTCCTGGGCTGCCATGCACACAAATGCACCGCAACTTTGGCTTAAGTATTGCTGAAGATCGGCATGTAAAAATATTATCTCTGGTTCATTCAAATGAACTTTTAtattattat                                                                                                                               <  3:721017/151‑1 (MQ=255)
                    ggggCGCTGTCAGAATTGCGTTCCCTTTGTTCAATCACTTCCTGGGCTGCCATGCACACAAATGCACCGCAACTTTGGCTTAAGTATTGCTGAAGATCGGCATGTAAAAATATTATCTCTGGTTCATTCAAATGAACTTTTAtattattat                                                                                                                               <  7:199081/151‑1 (MQ=255)
                     gggCGCTGTCAGAATTGCTTTCCCTTTGTTCAATCACTTCCTGGGCTGCCATGCACACAAATGCACCGCAACTTTGGCTTAAGTATTGCTGAAGATCGGCATGTAAAAATATTATCTCTGGTTCATTCAAATGAACTTTTATattattatt                                                                                                                              <  3:1390585/151‑1 (MQ=255)
                         gcTGTCAGAATTGCTTTCCCTTTGTTCAACCACTTCCTGGGCTGCCATGCACACAAATGCACCGCAACTTTGGCTTAAGTATTGCTGAAGATCGGCATGTAAAAATATTATCTCTGGTTCATTCAAATGAACTTTTATATTATTATTCGAc                                                                                                                          <  4:1742075/151‑1 (MQ=255)
                           tGTCAGAATTGCTTTCCCTTTGTTCAATCACTTCCTGGGCTGCCATGCACACAAATGCACCGCAACTTTGGCTTAAGTATTGCTGAAGATCGGCATGTAAAAATATTATCTCTGGTTCATTCAAATGAACTTTTATATTATTATTCGACAt                                                                                                                        >  3:641869/1‑151 (MQ=255)
                              aaGAATTGCTTTCCCTTTGTTCAATCACTTCCTGGGCTGCCATGCACACAAATGCACCGCAACTTTGGCTTAAGTATTGCTGAAGATCGGCATGTAAAAATATTATCTCTGGTTCATTCAAATGAACTTTTATATTATTATTCGACATTtc                                                                                                                     <  6:497966/150‑1 (MQ=255)
                               aGAATTGCTTTCCCTTTGTTCAATCACTTCCTGGGCTGCCATGCACACAAATGCACCGCAACTTTGGCTTAAGTATTGCTGAAGATCGGCATGTAAAAATATTATCTCTGGTTCATTCAAATGAACTTTTATATTATTATTCGACATTtct                                                                                                                    <  8:381074/151‑1 (MQ=255)
                                gAATTGCTTTCCCTTTGTTCAATCACTTCCTGGGCTGCCATGCACACAAATGCACCGCAACTTTGGCTTAAGTATTGCTGAAGATCGGCATGTAAAAATATTATCTCTGGTTCATTCAAATGAACTTTTATATTATTATTCGACATTtctc                                                                                                                   >  8:1350194/1‑151 (MQ=255)
                                  aTTGCTTTCCCTTTGTTCTATGACTTCCTGGGCTGCCATGCTCACAAATGCAGCTCAACTTTGGCTTAAGTATTGCTGTAGATCGGCATGTAAAAATATTATCTCTGGTTCATTCAAATGAACTTTTATATTATTATTCGAGATTTCTccc                                                                                                                 <  6:941956/151‑1 (MQ=255)
                                  aTTGCTTTCCCTTTGTTCAATCACTTCCTGGGCTGCCATGCACACAAATGCACCGCAACTTTGGCTTAAGTATTGCTGAAGATCGGCATGTAAAAATATTATCTCTGGTTCATTCAAATGAACTTTTATATTATTATTCGACATTTCTccc                                                                                                                 >  3:1555637/1‑151 (MQ=255)
                                    tGCTTTCCCTTTGTTCAATCACTTCCTGGGCTGCCATGCACACAAATGCACCGCAACTTTGGCTTAAGTATTCCTGAAGATCCGCATGAAAAAATATTATCTCTGGTTAATTAAAATCAACTTTTATATTATTATACTACATTTATACCa                                                                                                                >  6:1923087/1‑150 (MQ=255)
                                     gCTTTCCCTTTGTTCAATCACTTCCTGGGCTGCCATGCACACAAATGCACCGCAACTTTGGCTTAAGTATTGCTGAAGATCGGCATGTAAAAATATTATCTCTGGTTCATTCAAATGAACTTTTATATTATTATTCGACATTTCTCCCATg                                                                                                              <  5:1759270/151‑1 (MQ=255)
                                            cTTTGTTCAATCACTTCCTGGGCTGCCATGCACACAAATGCACCGCAACTTTGGCTTAAGTATTGCTGAAGATCGGCATGTAAAAATATTATCTCTGGTTCATTCAAATGAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTc                                                                                                         >  7:653573/1‑149 (MQ=255)
                                                gTTCAATCACTTCCTGGGCTGCCATGCACACAAATGCACCGCAACTTTGGCTTAAGTATTGCTGAAGATCGGCATGTAAAAATATTATCTCTGGTTCATTCAAATGAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTCAGAAtt                                                                                                   <  6:1780185/151‑1 (MQ=255)
                                                gTTCAATCACTTCCTGGGCTGCCATGCACACAAATGCACCGCAACTTTGGCTTAAGTATTGCTGAAGATCGGCATGTAAAAATATTATCTCTGGTTCATTCAAATGAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTCAGAAtt                                                                                                   >  5:1289235/1‑151 (MQ=255)
                                                  tCAATCACTTCCTGGGCTGCCATGCACACAAATGCACCGCAACTTTGGCTTAAGTATTGCTGAAGATCGGCATGTAAAAATATTATCTCTGGTTCATTCAAATGAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTCAGAATTa                                                                                                  <  7:1590459/150‑1 (MQ=255)
                                                      tCACTTCCTGGGCTGCCATGCACACAAATGCACCGCAACTTTGGCTTAAGTATTGCTGAAGATCGGCATGTAAAAATATTATCTCTGGTTCATTCAAATGAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTCAGAATTAAAACt                                                                                             >  8:1078426/1‑151 (MQ=255)
                                                           tCCTCGGCTGCCATGCACACAAATGCACCGCAACTTTGGCTTATGTATTGCTGAAGATCGGCATGTAAAAATATTATCTCTGGTTCATTCAAATGAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTCAGAATTAAAACTATTa                                                                                         <  3:433436/150‑1 (MQ=255)
                                                                 gCTGCCATGCACACAAATGCACCGCAACTTTGGCTTAAGTATTGCTGAAGATCGGCATGTAAAAATATTATCTCTGGTTCATTCAAATGAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTCAGAATTAAAACTATTAATGacac                                                                                  <  4:1514459/151‑1 (MQ=255)
                                                                    gCCATGCACACAAATGCACCGCAACTTTGGCTTAAGTATTGCTGAAGATCGGCATGTAAAAATATTATCTCTGGTTCATTCAAATGAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTCAGAATTAAAACTATTAATGACACg                                                                                 <  3:1002554/149‑1 (MQ=255)
                                                                    gCCATGCACACAAATGCACCGCAACTTTGGCTTAAGTATTGCTGAAGATCGGCATGTAAAAATATTATCTCTGGTTCATTCAAATGAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTCAGAATTAAAACTATTAATGACACg                                                                                 <  7:16914/149‑1 (MQ=255)
                                                                     ccATGCACACAAATGCACCGCAACTTTGGCTTAAGTATTGCTGAAGATCGGCATGTAAAAATATTATCTCTGGTTCATTCAAATGAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTCAGAATTAAAACTATTAATGACACGCtt                                                                              >  7:1140601/1‑151 (MQ=255)
                                                                     ccATGCACACAAATGCACCGCAACTTTGGCTTAAGTATTGCTGAAGATCGGCATGTAAAAATATTATCTCTGGTTCATTCAAATGAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTCAGAATTAAAACTATTAATGACACGCtt                                                                              <  4:561121/151‑1 (MQ=255)
                                                                       aTGCACACAAATGCACCGCAACTTTGGCTTAAGTATTGCTGAAGATCGGCATGTAAAAATATTATCTCTGGTTCATTCAAATGAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTCAGAATTAAAACTATTAATGACACGCTTaa                                                                            <  6:1590236/151‑1 (MQ=255)
                                                                               aaaTGCACCGCAACTTTGGCTTAAGTATTGCTGAAGATCGGCATGTAAAGATATTATCTCTGGTTCATTCAAATGAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTCAGAATTAAAACTATTAATGACACGCTTAAGTGAAttt                                                                    <  5:265732/151‑1 (MQ=255)
                                                                               aaaTGCACCGCAACTTTGGCTTAAGTATTGCTGAAGATCGGCATGTAAAAATATTATCTCTGGTTCATTCAAATGAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTCAGAATTAAAACTATTAATGACACGCTTAAGTGAAttt                                                                    >  8:816790/1‑151 (MQ=255)
                                                                                aaTGCACCGCAACTTTGGCTTAAGTATTGCTGAAGATCGGCATGTAAAAATATTATCTCTGGTTCATTCAAATGAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTCAGAATTAAAACTATTAATGACACGCTTAAGTGAAtttt                                                                   >  2:391055/1‑151 (MQ=255)
                                                                                  tGCACCGCAACTTTGGCTTAAGTATTGCTGAAGATCGGCATGTAAAAATATTATCTCTGGTTCATTCAAATGAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTCAGAATTAAAACTATTAATGACACGCTTAAGTGAATTTTCt                                                                 >  7:761345/1‑151 (MQ=255)
                                                                                   gCACCGCAACTTTGGCTTAAGTATTGCTGAAGATCGGCATGTAAAAATATTATCTCTGGTTCATTCAAATGAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTCAGAATTAAAACTATTAATGACACGCTTAAGTGAATTTTCtt                                                                >  1:1608866/1‑151 (MQ=255)
                                                                                      ccGCAACTTTGGCTTAAGTATTGCTGAAGATCGGCATGTAAAAATATTATCTCTGGTTCATTCAAATGAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTCAGAATTAAAACTATTAATGACACGCTTAAGTGAATTTTCTTTAt                                                             <  8:631693/151‑1 (MQ=255)
                                                                                      ccGCAACTTTGGCTTAAGTATTGCTGAAGATCGGCATGTAAAAATATTATCTCTGGTTCATTCAAATGAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTCAGAATTAAAACTATTAATGACACGCTTAAGTGAATTTTCTTTAt                                                             >  2:1219111/1‑151 (MQ=255)
                                                                                         cAACTTTGGCTTAAGTATTGCTGAAGATCGGCATGTAAAAATATTATCTCTGGTTCATTCAAATGAACTTTTATATTATTAATCGACATTTCTCCCATGAGTTCAGAATTAAAACTATTAATGACACGCTTAAGTGAATTTTCTTTATCAt                                                          <  4:13805/151‑1 (MQ=255)
                                                                                            cTTTGGCTTAAGTATTGCTGAAGATCGGCATGTAAAAATATTATCTCTGGTTCATTCAAATGAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTCAGAATTAAAACTATTAATGACACGCTTAAGTGAATTTTCTTTATCATAGc                                                       >  4:399333/1‑151 (MQ=255)
                                                                                                ggCTTAAGTATTGCTGAAGATCGGCATGTAAAAATATTATCTCTGGTTCATTCAAATGAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTCAGAATTAAAACTATTAATGACACGCTTAAGTGAATTTTCTTTATCATAGCCt                                                     >  1:1353016/1‑149 (MQ=255)
                                                                                                  cTTAAGTATTGCTGAAGATCGGCATGTAAAAATATTATCTCTGGTTCATTCAAATGAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTCAGAATTAAAACTATTAATGACACGCTTAAGTGAATTTTCTTTATCATAGCCTAGTg                                                 >  1:1005740/1‑151 (MQ=255)
                                                                                                              tGAAGATCGGCATGTAAAAATATTATCTCTGGTTCATTCAAATGAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTCAGAATTAAAACTATTAATGACACGCTTAAGTGAATTTTCTTTATCATAGCCTAGTGCACGCAATGAGt                                     >  7:1287419/1‑151 (MQ=255)
                                                                                                                aaGATCGGCATGTAAAAATATTATCTCTGGTTCATTCAAATGAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTCAGAATTAAAACTATTAATGACACGCTTAAGTGAATTTTCTTTATCATAGCCTAGTGCACGCAATGAGTTg                                   >  1:97678/1‑151 (MQ=255)
                                                                                                                aaGATCGGCATGTAAAAATATTATCTCTGGTTCATTCAAATGAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTCAGAATTAAAACTATTAATGACACGCTTAAGTGAATTTTCTTTATCATAGCCTAGTGCACGCAATGAGTTg                                   >  6:1517682/1‑151 (MQ=255)
                                                                                                                aaGATCCGCATGTAAAAATATTATCTCTGGTTCATTCAAATGAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTCAGAATTAAAACTATTAATGACACGCTTAAGTGAATTTTCTTTATCATAGCCTAGTGCACGCAATGAGTTg                                   <  6:747542/151‑1 (MQ=255)
                                                                                                                 aGATCGGCATGTAAAAATAATATCTCTGGTTCATTCAAATCAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTCAGAATTAAAACTATTAATGACACGCTTAAGTGAATTTTCTTTATCATAGCCTAGTGCACGCAATCAGTTg                                   >  4:1808703/1‑150 (MQ=255)
                                                                                                                  gATCGGCATGTAAAAATATTATCTCTGGTTCATTCAAATGAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTCAGAATTAAAACTATTAATGACACGCTTAAGTGAATTTTCTTTATCATAGCCTAGTGCACGCAATGAGTTGaa                                 <  5:196010/151‑1 (MQ=255)
                                                                                                                  gATCGGCATGTAAAAATATTATCTCTGGTTCATTCAAATGAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTCAGAATTAAAACTATTAATGACACGCTTAAGTGAATTTTCTTTATCATAGCCTAGTGCACGCAATGAGTTGaa                                 <  1:1722639/151‑1 (MQ=255)
                                                                                                                   aTCGGCATGTAAAAATATTATCTCTGGTTCATTCAAATGAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTCAGAATTAAAACTATTAATGACACGCTTAAGTGAATTTTCTTTATCATAGCCTAGTGCACGCAATGAGTTGAat                                >  7:456928/1‑151 (MQ=255)
                                                                                                                     cGGCATGTAAAAATATTATCTCTGGTTCATTCAAATGAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTCAGAATTAAAACTATTAATGACACGCTTAAGTGAATTTTCTTTATCATAGCCTAGTGCACGCAATGAGTTGAatat                              <  4:505823/151‑1 (MQ=255)
                                                                                                                     cGGCATGTAAAAATATTATCTCTGGTTCATTCAAATGAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTCAGAATTAAAACTATTAATGACACGCTTAAGTGAATTTTCTTTATCATAGCCTAGTGCACGCAATGAGTTGAatat                              <  5:931833/151‑1 (MQ=255)
                                                                                                                      ggCATGTAAAAATATTATCTCTGGTTCATTCAAATGAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTCAGAATTAAAACTATTAATGACACGCTTAAGTGAATTTTCTTTATCATAGCCTAGTGCACGCAATGAGTTGAATATg                             >  6:1381362/1‑151 (MQ=255)
                                                                                                                      ggCATGTAAAAATATTATCTCTGGTTCATTCAAATGAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTCAGAATTAAAACTATTAATGACACGCTTAAGTGAATTTTCTTTATCATAGCCTAGTGCACGCAATGAGTTGAATATg                             <  1:1232908/151‑1 (MQ=255)
                                                                                                                       gCATGTAAAAATATTATCTCTGGTTCATTCAAATGAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTCAGAATTAAAACTATTAATGACACGCTTAAGTGAATTTTCTTTATCATAGCCTAGTGCACGCAATGAGTTGAATATg                             >  7:1430107/1‑150 (MQ=255)
                                                                                                                        cATGTAAAAATATTATCTCTGGTTCATTCAAATGAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTCAGAATTAAAACTATTAATGACACGCTTAAGTGAATTTTCTTTATCATAGCCTAGTGCACGCAATGAGTTGAATATGAc                           >  7:846846/1‑151 (MQ=255)
                                                                                                                         aTGTAAAAATATTATCTCTGGTTCATTCAAATGAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTCAGAATTAAAACTATTAATGACACGCTTAAGTGAATTTTCTTTATCATAGCCTAGTGCACGCAATGAGTTGAATATGACg                          <  8:1755621/151‑1 (MQ=255)
                                                                                                                         aTGTAAAAATATTATCTCTGGTTCATTCAAATGAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTCAGAATTAAAACTATTAATGACACGCTTAAGTGAATTTTCTTTATCATAGCCTAGTGCACGCAATGAGTTGAATATGACg                          >  5:1562938/1‑151 (MQ=255)
                                                                                                                         aTGTAAAAATATTATCTCTGGTTCATTCAAATGAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTCAGAATTAAAACTATTAATGACACGCTTAAGTGAATTTTCTTTATCATAGCCTAGTGCACGCAATGAGTTGAATATGACg                          >  8:203325/1‑151 (MQ=255)
                                                                                                                          tGTAAAAATATTATCTCTGGTTCATTCAAATGAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTCAGAATTAAAACTATTAATGACACGCTTAAGTGAATTTTCTTTATCATAGCCTAGTGCACGCAATGAGTTGAATATGACGc                         >  5:606444/1‑151 (MQ=255)
                                                                                                                          tGTAAAAATATTATCTCTGGTTCATTCAAATGAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTCAGAATTAAAACTATTAATGACACGCTTAAGTGAATTTTCTTTATCATAGCCTAGTGCACGCAATGAGTTGAATATGACGc                         >  1:911420/1‑151 (MQ=255)
                                                                                                                           gTAAAAATATTATCTCTGGTTCATTCAAATGAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTCAGAATTAAAACTATTAATGACACGCTTAAGTGAATTTTCTTTATCATAGCCTAGTGCACGCAATGAGTTGAATATGACGc                         <  8:1628249/150‑1 (MQ=255)
                                                                                                                           gTAAAAATATTATCTCTGGTTCATTCAAATGAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTCAGAATTAAAACTATTAATGACACGCTTAAGTGAATTTTCTTTATCATAGCCTAGTGCACGCAATGAGTTGAATATGACGCa                        <  7:972063/151‑1 (MQ=255)
                                                                                                                            tAAAAATATTATCTCTGGTTCATTCAAATGAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTCAGAATTAAAACTATTAATGACACGCTTAAGTGAATTTTCTTTATCATAGCCTAGTGCACGCAATGAGTTGAATATGACGCAt                       >  8:894873/1‑151 (MQ=255)
                                                                                                                             aaaaaTATTATCTCTGGTTCATTCAAATGAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTCAGAATTAAAACTATTAATGACACGCTTAAGTGAATTTTCTTTATCATAGCCTAGTGCACGCAATGAGTTGAATATGACGCAt                       <  5:1064096/150‑1 (MQ=255)
                                                                                                                              aaaaTATTATCTCTGGTTCATTCAAATGAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTCAGAATTAAAACTATTAATGACACGCTTAAGTGAATTTTCTTTATCATAGCCTAGTGCACGCAATGAGTTGAATATGACGCAttt                     <  8:302092/151‑1 (MQ=255)
                                                                                                                              aaaaTATTATCTCTGGTTCATTCAAATGAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTCAGAATTAAAACTATTAATGACACGCTTAAGTGAATTTTCTTTATCATAGCCTAGTGCACGCAATGAGTTGAATATGACGCAttt                     <  4:924764/151‑1 (MQ=255)
                                                                                                                              aaaaTATTATCTCTGGTTCATTCAAATGAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTCAGAATTAAAACTATTAATGACACGCTTAAGTGAATTTTCTTTATCATAGCCTAGTGCACGCAATGAGTTGAATATGACGCAttt                     >  2:191582/1‑151 (MQ=255)
                                                                                                                              aaaaTATTATCTCTGGTTAATTAAAATTAACTTTTATATTATTATTCGACATTTCTGCCATGAGTTAAGAATTAAAACTATTAATGACTCGGTTAAGTGAATTTTCTTTATCATATCCTAGTGGACTCAATTAGTTGAATATGACGCAttt                     <  8:1520190/151‑1 (MQ=255)
                                                                                                                               aaaTATTATCTCTGGTTCATTCAAATGAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTCAGAATTAAAACTATTAATGACACGCTTAAGTGAATTTTCTTTATCATAGCCTAGTGCACGCAATGAGTTGAATATGACGCAtttt                    >  1:1458449/1‑151 (MQ=255)
                                                                                                                               aaaTATTATCTCTGGTTCATTCAAATGAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTCAGAATTAAAACTATTAATGACACGCTTAAGTGAATTTTCTTTATCATAGCCTAGTGCACGCAATGAGTTGAATATGACGCAtttt                    >  2:1490561/1‑151 (MQ=255)
                                                                                                                                aaTATTATCTCTGGTTCATTCAAATGAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTCAGAATTAAAACTATTAATGACACGCTTAAGTGAATTTTCTTTATCATAGCCTAGTGCACGCAATGAGTTGAATATGACGCAtttt                    >  8:488204/1‑150 (MQ=255)
                                                                                                                                 atatTATCTCTGGTTCATTCAAATGAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTCAGAATTAAAACTATTAATGACACGCTTAAGTGAATTTTCTTTATCATAGCCTAGTGCACGCAATGAGTTGAATATGACGCATTTTAt                  >  3:1848686/1‑151 (MQ=255)
                                                                                                                                 atatTATCTCTGGTTCATTCAAATGAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTCAGAATTAAAACTATTAATGACACGCTTAAGTGAATTTTCTTTATCATAGCCTAGTGCACGCAATGAGTTGAATATGACGCATTTTAt                  >  3:1634269/1‑151 (MQ=255)
                                                                                                                                 atatTATCTCTGGTTCATTCAAATGAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTCAGAATTAAAACTATTAATGACACGCTTAAGTGAATTTTCTTTATCATAGCCTAGTGCACGCAATGAGTTGAATATGACGCATTTTAt                  <  2:719947/151‑1 (MQ=255)
                                                                                                                                     tatCTCTGGTTCATTCAAATGAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTCAGAATTAAAACTATTAATGACACGCTTAAGTGAATTTTCTTTATCATAGCCTAGTGCACGCAATGAGTTGAATATGACGCATTTTATTTg               <  3:314719/150‑1 (MQ=255)
                                                                                                                                     tatCTCTGGTTCATTCAAATGAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTCAGAATTAAAACTATTAATGACACGCTTAAGTGAATTTTCTTTATCATAGCCTAGTGCACGCAATGAGTTGAATATGACGCATTTTATTTGt              >  4:716821/1‑151 (MQ=255)
                                                                                                                                       tctcTGGTTCATTCAAATGAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTCAGAATTAAAACTATTAATGACACGCTTAAGTGAATTTTCTTTATCATAGCCTAGTGCACGCAATGAGTTGAATATGACGCATTTTATTTGt              >  1:1750062/1‑149 (MQ=255)
                                                                                                                                          gtGGTTCATTCAAATGAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTCAGAATTAAAACTATTAATGACACGCTTAAGTGAATTTTCTTTATCATAGCCTAGTGCACGCAATGAGTTGAATATGACGCATTTTATTTGTAGttt         <  2:1752405/150‑1 (MQ=255)
                                                                                                                                          ctGGTTCATTCAAATGAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTCAGAATTAAAACTATTAATGACACGCTTAAGTGAATTTTCTTTATCATAGCCTAGTGCACGCAATGAGTTGAATATGACGCATTTTATTTGTa             <  2:231661/147‑1 (MQ=255)
                                                                                                                                          ctGGTTCATTCAAATGAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTCAGAATTAAAACTATTAATGACACGCTTAAGTGAATTTTCTTTATCATAGCCTAGTGCACGCAATGAGTTGAATATGACGCATTTTATTTGTa             <  1:1855131/147‑1 (MQ=255)
                                                                                                                                          ctGGTTCATTCAAATGAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTCAGAATTAAAACTATTAATGACACGCTTAAGTGAATTTTCTTTATCATAGCCTAGTGCACGCAATGAGTTGAATATGACGCATTTTATTTGTAg            <  5:1464869/148‑1 (MQ=255)
                                                                                                                                          ctGGTTCATTCAAATGAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTCAGAATTAAAACTATTAATGACACGCTTAAGTGAATTTTCTTTATCATAGCCTAGTGCACGCAATGAGTTGAATATGACGCATTTTATTTGTAGttt         >  2:140173/1‑151 (MQ=255)
                                                                                                                                           tGGTTCATTCAAATGAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTCAGAATTAAAACTATTAATGACACGCTTAAGTGAATTTTCTTTATCATAGCCTAGTGCACGCAATGAGTTGAATATGACGCATTTTATTTGTAGttt         >  4:788815/1‑150 (MQ=255)
                                                                                                                                            ggTTCATTCAAATGAACTTTTATATTATTATTCGACATTTCTCCGATGAGTTCAGAATTAAAACTATTAATGACACGCTTAAGTGAATTTTCTTTATCATAGCCTAGTGCACGCAATGAGTTGAATATGACGCATTTTATTTGTAGttt         <  3:463771/149‑1 (MQ=255)
                                                                                                                                            ggTTCATTCAAATGAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTCAGAATTAAAACTATTAATGACACGCTTAAGTGAATTTTCTTTATCATAGCCTAGTGCACGCAATGAGTTGAATATGACGCATTTTATTTGTAGTttat       <  7:568909/151‑1 (MQ=255)
                                                                                                                                             gTTCATTCAAATGAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTCAGAATTAAAACTATTAATGACACGCTTAAGTGAATTTTCTTTATCATAGCCTAGTGCACGCAATGAGTTGAATATGACGCATTTTATTTGTAGTttatt      <  2:1653852/151‑1 (MQ=255)
                                                                                                                                               tCATTCAAATGAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTCAGAATTAAAACTATTAATGACACGCTTAAGTGAATTTTCTTTATCATAGCCTAGTGCACGCAATGAGTTGAGTATGACGCATTTTATTTGTAGTTtattat    >  1:1122729/1‑151 (MQ=255)
                                                                                                                                               tCATTCAAATGAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTCAGAATTAAAACTATTAATGACACGCTTAAGTGAATTTTCTTTATCATAGCCTAGGGCACGCAATGAGTTGAATATGACGCATTTTATTTGTAGCttatta     <  1:311992/150‑1 (MQ=255)
                                                                                                                                                cATTCAAATGAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTCAGAATTAAAACTATTAATGACACGCTTAAGTGAATTTTCTTTATCATAGCCTAGTGCACGCAATGAGTTGAATATGACGCATTTTATTTGTAGTTTattatt   >  1:477318/1‑151 (MQ=255)
                                                                                                                                                cATTCAAATGAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTCAGAATTAAAACTATTAATGACACGCTTAAGTGAATTTTCTTTATCATAGCCTAGTGCACGCAATGAGTTGAATATGACGCATTTTATTTGTAGTTTattatt   <  4:667872/151‑1 (MQ=255)
                                                                                                                                                cATTCAAATGAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTCAGAATTAAAACTATTAATGACACGCTTAAGTGAATTTTCTTTATCATAGCCTAGTGCACGCAATGAGTTGAATATGACGCATTTTATTTGTAGTTTattatt   >  5:456549/1‑151 (MQ=255)
                                                                                                                                                cATTCAAATGAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTCAGAATTAAAACTATTAATGACACGATTAAGTGAATTTTCTTTATCATAGCCTAGTGCACTCAATGAGTTGAATATGACGCATTTTATTTGTAGTTtattat    <  6:385149/150‑1 (MQ=255)
                                                                                                                                                 aTTCAAATGAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTCAGAATTAAAACTATTAATGACACGCTTAAGTGAATTTTCTTTATCATAGCCTAGTGCACGCAATGAGTTGAATATGACGCATTTTATTTGTAGTTTATTAttt  <  4:251549/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GGTTTTTTAATAACGTATAGGGGGCGCTGTCAGAATTGCTTTCCCTTTGTTCAATCACTTCCTGGGCTGCCATGCACACAAATGCACCGCAACTTTGGCTTAAGTATTGCTGAAGATCGGCATGTAAAAATATTATCTCTGGTTCATTTAAATGAACTTTTATATTATTATTCGACATTTCTCCCATGAGTTCAGAATTAAAACTATTAATGACACGCTTAAGTGAATTTTCTTTATCATAGCCTAGTGCACGCAATGAGTTGAATATGACGCATTTTATTTGTAGTTTATTATTT  >  DH1/1493233‑1493528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑