Predicted mutation
evidence seq id position mutation annotation gene description
RA DH1 1,709,744 G→T G4C (GGT→TGT)  yegR → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH11,709,7440GT99.0% 1908.6 / 3.8 508G4C (GGT→TGT) yegRhypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (4/1);  new base T (275/228);  total (279/229)
Fisher's exact test for biased strand distribution p-value = 3.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 521 total aligned reads displayed.

AGTTACCCTGAAGGGCCTCAATGTCCGTAATTCCTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAAGGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCGGTAAATTTGGTCTTGCATATAAAAGTAATATTCAGCGTAAACTCGATAACCAATACTACA  >  DH1/1709596‑1709891
                                                                                                                                                    |                                                                                                                                                   
aGTTACCCTGAAGGGCCTCAATGTCCGTAATTCCTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGt                                                                                                                                                   >  1:1027483/1‑151 (MQ=255)
aGTTACCCTGAAGGGCCTCAATGTCCGTAATTCCTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGt                                                                                                                                                   <  5:465492/151‑1 (MQ=255)
 gTTACCCTGAAGGGCCTCAATGTCCGTAATTCCTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAAtgtg                                                                                                                                                  >  6:862815/1‑151 (MQ=255)
  ttACCCTGAAGGGCCTCAATGTCCGTAATTCCTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAAtgtg                                                                                                                                                  >  3:1753676/1‑150 (MQ=255)
    aCCCTGAAGGGCCTCAATGTCCGTAATTCCTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAAtgtg                                                                                                                                                  >  4:1900055/1‑148 (MQ=255)
        tGAAGGGCCTCAATGTCCGTAATTCCTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGa                                                                                                                                            >  3:736103/1‑150 (MQ=255)
         gAAGGGCCTGAATGTCCGTAATTCCTACTTATGTAGGAAATGTTGTACAGAAGATTTATTATAATCCTATTCAATTATAATAATCATGGCATTATTATATTTAATCACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGaaa                                                                                                                                          <  5:992873/151‑1 (MQ=255)
           aGGGCCTCAATGTCCGTAATTCCTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGaaaa                                                                                                                                         >  1:1290919/1‑150 (MQ=255)
               ccTCAATGTCCGTAATTCCTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGAt                                                                                                                                     >  6:425794/1‑150 (MQ=255)
                  cAATGTCCGTAATTCCTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAgct                                                                                                                                 <  2:883320/151‑1 (MQ=255)
                       tCCGTAATTCCTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCtat                                                                                                                            >  1:1214860/1‑151 (MQ=255)
                       tCCGTAATTCCTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCtat                                                                                                                            >  7:1643530/1‑151 (MQ=255)
                          gTAATTCCTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTA‑TATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATc                                                                                                                         >  1:900235/1‑150 (MQ=255)
                           tAATTCCTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATc                                                                                                                         >  4:1749549/1‑150 (MQ=255)
                            aaTTCCTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCat                                                                                                                       <  3:25803/151‑1 (MQ=255)
                            aaTTCCTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCat                                                                                                                       <  2:473123/151‑1 (MQ=255)
                            aaTTCCTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCa                                                                                                                        <  5:1808698/150‑1 (MQ=255)
                                ccTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCattaat                                                                                                                   >  3:55/1‑151 (MQ=255)
                                ccTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCattaat                                                                                                                   >  3:1108575/1‑151 (MQ=255)
                                 cTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCattaatt                                                                                                                  >  3:812136/1‑151 (MQ=255)
                                 cTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCattaatt                                                                                                                  >  8:534682/1‑151 (MQ=255)
                                  tACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCattaatt                                                                                                                  >  3:384462/1‑150 (MQ=255)
                                    cTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGt                                                                                                               >  3:475673/1‑151 (MQ=255)
                                    cTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGt                                                                                                               >  7:566604/1‑151 (MQ=255)
                                    attATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTAAAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTCGt                                                                                                               <  5:869592/150‑1 (MQ=255)
                                      tATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTAt                                                                                                             >  1:737339/1‑151 (MQ=255)
                                       aTGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTAtt                                                                                                            >  2:402308/1‑151 (MQ=255)
                                         gTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGTTTTTTAATGGGGGAATTTGAGATGAAAAAGATATCTGCTATATCATTAATTAGTAttt                                                                                                           <  7:1304966/150‑1 (MQ=255)
                                           aGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTAttttt                                                                                                         <  5:1628283/150‑1 (MQ=255)
                                                aTGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATg                                                                                                   <  2:1610090/151‑1 (MQ=255)
                                                    tgtACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCt                                                                                                <  5:1059172/150‑1 (MQ=255)
                                                     gtACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTgg                                                                                              >  7:711363/1‑151 (MQ=255)
                                                      tACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGt                                                                                             >  4:101930/1‑151 (MQ=255)
                                                         aGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTtgt                                                                                          >  6:1243564/1‑151 (MQ=255)
                                                         aGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTtgt                                                                                          <  8:1223325/151‑1 (MQ=255)
                                                          gatcATTTATTATAATCCTTTTAAATTATAATTATGATGCCATTATTATATTTAAACACTTGAGAGTGTCGTTGGTATTTTATGGGGGTATGTGAGATGAATTAGATAGGTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTtgt                                                                                          <  4:693791/147‑1 (MQ=255)
                                                           aaCATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTATTATTTTTATTATGTCTGGTtgtgc                                                                                        >  7:20819/1‑151 (MQ=255)
                                                           aaCATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTtgtgc                                                                                        >  1:1084724/1‑151 (MQ=255)
                                                           aaCATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTtgtgc                                                                                        >  6:716058/1‑151 (MQ=255)
                                                           aaCATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTtgtgc                                                                                        >  8:1731670/1‑151 (MQ=255)
                                                              aTTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTtgtgctgt                                                                                     >  1:27595/1‑151 (MQ=255)
                                                              aTTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTtgtgctgt                                                                                     >  4:567577/1‑151 (MQ=255)
                                                               tttATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTtgtgctgtg                                                                                    >  2:1870855/1‑151 (MQ=255)
                                                                ttattaTAATCCTATTCAATTATAATGATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCCTTAATTAGTATTTTTATTATGTCTGGTtgtgctgtg                                                                                    <  7:881715/150‑1 (MQ=255)
                                                                  attatAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCa                                                                                  <  4:1412638/150‑1 (MQ=255)
                                                                    tataATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATa                                                                                >  6:623835/1‑150 (MQ=255)
                                                                    tataATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATa                                                                                <  7:1007783/150‑1 (MQ=255)
                                                                     ataATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGATTGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTATGGTTTTGGTGTGGATAat                                                                              >  5:1491040/1‑151 (MQ=255)
                                                                       aaTCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAatg                                                                             >  7:1907118/1‑150 (MQ=255)
                                                                       aaTCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAatg                                                                             >  4:4103/1‑150 (MQ=255)
                                                                         tCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAatgatt                                                                          >  4:1303529/1‑150 (MQ=255)
                                                                          ccTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAAtgatga                                                                         <  4:1136079/151‑1 (MQ=255)
                                                                             aTTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGAc                                                                      >  7:43464/1‑151 (MQ=255)
                                                                              ttCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGAc                                                                      >  2:1169153/1‑150 (MQ=255)
                                                                              ttCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGAc                                                                      <  1:1724986/150‑1 (MQ=255)
                                                                              ttCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGAc                                                                      >  8:1963073/1‑150 (MQ=255)
                                                                               tCATTTATACTCATCATGCCCTTATTATATTTACACACTAGCGCGTGGCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATCGCTGCTATATCATTAATTAGTATTTTTATTATGTTTGGTTGTGCGGTGCATAATGATGAGACa                                                                     <  6:1310571/150‑1 (MQ=255)
                                                                               tCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACa                                                                     >  6:1921855/1‑150 (MQ=255)
                                                                                  aTTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGt                                                                  >  3:172533/1‑150 (MQ=255)
                                                                                  aTTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGt                                                                  >  1:416240/1‑150 (MQ=255)
                                                                                  aTTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTa                                                                 <  6:1538568/151‑1 (MQ=255)
                                                                                  aTTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCAATAATTAGTATTTTTATTATGTCTGGTTGTGGTGTGCATTATGATGAGACAAGt                                                                  >  5:1409632/1‑150 (MQ=255)
                                                                                   ttATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTAt                                                                >  7:1045750/1‑151 (MQ=255)
                                                                                   ttATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTAt                                                                >  8:569833/1‑151 (MQ=255)
                                                                                       aataatCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCGGt                                                            >  4:1513200/1‑151 (MQ=255)
                                                                                       aataatCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGAAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCGGt                                                            <  2:1443034/151‑1 (MQ=255)
                                                                                        ataatCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCGGTa                                                           <  4:841731/151‑1 (MQ=255)
                                                                                              aTGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAATTATCGGTAAAtttt                                                     >  4:31350/1‑150 (MQ=255)
                                                                                              aTGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCGGTAAATTTg                                                     >  7:1766368/1‑151 (MQ=255)
                                                                                              aTGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCGGTAAATTTg                                                     >  3:1896755/1‑151 (MQ=255)
                                                                                                  cATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCGGTAAATTTGGTCt                                                 >  1:58657/1‑151 (MQ=255)
                                                                                                   attattATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCGGTAAATTTGGTCtt                                                >  8:104865/1‑151 (MQ=255)
                                                                                                   attattATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCGGTAAATTTGGTCtt                                                >  3:158125/1‑151 (MQ=255)
                                                                                                     tattatATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCGGTAAATTTGGTCTTg                                               <  8:417964/150‑1 (MQ=255)
                                                                                                         atatTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCAT‑ATGATGAGACAAGTATCGGTAAATTTGGTCTTGCatat                                          >  8:1588463/1‑150 (MQ=255)
                                                                                                           atTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCGGTAAATTTGGTCTTGCAtata                                         >  5:1301006/1‑150 (MQ=255)
                                                                                                           atTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCGGTAAATTTGGTCTTGCATATaa                                        <  5:630058/151‑1 (MQ=255)
                                                                                                           atTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCGGTAAATTTGGTCTTGCATATaa                                        <  1:1870892/151‑1 (MQ=255)
                                                                                                               aaaCACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCGGTAAATTTGGTCTTGCATATAAAAg                                     <  2:1298901/150‑1 (MQ=255)
                                                                                                               aaaCACTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCGGTAAATTTGGTCTTGCATATAAAAGt                                    >  6:266112/1‑151 (MQ=255)
                                                                                                                 acacTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCGGTAAATTTGGTCTTGCATATAAAAGTaa                                  <  8:812988/151‑1 (MQ=255)
                                                                                                                  cacTAGAGAGTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCGGTAAATTTGGTCTTGCATATAAAAGTAat                                 >  7:1451556/1‑151 (MQ=255)
                                                                                                                       gagagTGTCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCGGTAAATTTGGTCTTGCATATAAAAGTAATATTCa                            <  8:1107677/151‑1 (MQ=255)
                                                                                                                             gtCGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCGGTAAATTTGGTCTTGCATATAAAAGTAATATTCAGCGTa                       <  2:224724/150‑1 (MQ=255)
                                                                                                                               cGTTGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCGGTAAATTTGGTCTTGCATATAAAAGTAATATTCAGCGTAAAc                    <  2:1032138/151‑1 (MQ=255)
                                                                                                                                gTTGGTATTTAATGTGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCGGTAAATTTGGTCTTGCATATAAAAGTAATATTCAGCGTAAACt                   <  3:1017766/151‑1 (MQ=255)
                                                                                                                                 ttGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCGGTAAATTTGGTCTTGCATATAAAAGTAATATTCAGCGTAAACTc                  >  4:1618447/1‑151 (MQ=255)
                                                                                                                                  tGGTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCGGTAAATTTGGTCTTGCATATAAAAGTAATATTCAGCGTAAACTc                  <  1:66110/150‑1 (MQ=255)
                                                                                                                                  tGGTATTTAATGGGGCAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGATACAAGTATAGCTAAATTTGGTCTTGCATATAAAAATAAAATTAAGCGTAAACTc                  >  2:1376003/1‑150 (MQ=255)
                                                                                                                                   ggTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCGGTAAATTTGGTCTTGCATATAAAAGTAATATTCAGCGTAAACTCg                 >  5:18943/1‑150 (MQ=255)
                                                                                                                                   ggTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCGGTAAATTTGGTCTTGCATATAAAAGTAATATTCAGCGTAAACTCg                 >  5:1981966/1‑150 (MQ=255)
                                                                                                                                    gTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCGGTAAATTTGGTCTTGCATATAAAAGTAATATTCAGCGTAAACTCGAt               >  2:651009/1‑151 (MQ=255)
                                                                                                                                    gTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCGGTAAATTTGGTCTTGCATATAAAAGTAATATTCAGCGTAAACTCGAt               >  1:1489662/1‑151 (MQ=255)
                                                                                                                                    gTATTTAATGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCGGTAAATTTGGTCTTGCATATAAAAGTAATATTCAGCGTAAACTCGAt               <  7:348372/151‑1 (MQ=255)
                                                                                                                                      aTTTAATGGGGGAATGTGAGATGTAAATGATATCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATTATGAGACAAGTATCGGTAAATTTGGTCTTGCATATAAAAGTAATATTGAGCGTAAACTGGATa              <  6:1680068/150‑1 (MQ=255)
                                                                                                                                          aaTGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCGGTAAATTTGGTCTTGCATATAAAAGTAATATTCAGCGTAAACTCGATAACCa          >  4:898822/1‑150 (MQ=255)
                                                                                                                                           aTGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCGGTAAATTTGGTCTTGCATATAAAAGTAATATTCAGCGTAAACTCGATAACCAAt        >  3:874341/1‑151 (MQ=255)
                                                                                                                                            tGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCGGTAAATTTGGTCTTGCATATAAAAGTAATATTCAGCGTAAACTCGATAACCAAt        >  2:1753199/1‑150 (MQ=255)
                                                                                                                                            tGGGGGAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCGGTAAATTTGGTCTTGCATATAAAAGTAATATTCAGCGTAAACTCGATAACCAAt        >  8:1832806/1‑150 (MQ=255)
                                                                                                                                                 gAATGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCGGTAAATTTGGTCTTGCATATAAAAGTAATATTCAGCGTAAACTCGATAACCAAtactac   >  7:1134795/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
AGTTACCCTGAAGGGCCTCAATGTCCGTAATTCCTACTTATGTAGGAAATGTTGTACAGAACATTTATTATAATCCTATTCAATTATAATAATCATGCCATTATTATATTTAAACACTAGAGAGTGTCGTTGGTATTTAATGGGGGAAGGTGAGATGAAAAAGATAGCTGCTATATCATTAATTAGTATTTTTATTATGTCTGGTTGTGCTGTGCATAATGATGAGACAAGTATCGGTAAATTTGGTCTTGCATATAAAAGTAATATTCAGCGTAAACTCGATAACCAATACTACA  >  DH1/1709596‑1709891

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑