Predicted mutation
evidence seq id position mutation annotation gene description
RA DH1 1,755,118 G→A G14G (GGG→GGA)  cpsG → phosphomannomutase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH11,755,1180GA98.1% 1755.5 / 9.8 477G14G (GGG→GGA) cpsGphosphomannomutase
Reads supporting (aligned to +/- strand):  ref base G (6/3);  new base A (257/211);  total (263/214)
Fisher's exact test for biased strand distribution p-value = 7.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 480 total aligned reads displayed.

AAACGTCGCATCAGGCAATGAATGCGAAACCGCGGTGTAAATAACGACAAAAATAAAATTGGCCGCTTCGGTCAGGGCCAACTATTGCCTGAAAAAGGGTAACGATATGAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGGAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCATTGGTCGCGCCTATGGCGAATTTCTCAAACCGAAAACCATTGTGTTAGGCGGTGATGTCCGCCTCACCAGCGAAACCTTAAAACTGGCGCTGGCGAAAGGTTTAC  >  DH1/1754971‑1755263
                                                                                                                                                   |                                                                                                                                                 
aaaCGTCGCATCAGGCAATGAATGCGAAACCGCGGTGTAAATAACGACAAAAATAAAATAGGCCGCTTCGGTCAGGGCCAACTATTGCCTGAAAAAGGGTAACGATATGAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGaaa                                                                                                                                                 >  2:1116058/1‑150 (MQ=255)
   cGTCGCATCAGTCAATGAATGCGAAACCGCGGTGTAAATAACGACAAAAATAAAATTGGCCGCTTCGGTCAGGGCCAACTATTGCCTGAAAAAGGGTAACGATATGAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGAAAATTa                                                                                                                                             <  5:26775/151‑1 (MQ=255)
     tCGCATCAGGCAATGAATGCGAAACCGCGGTGTAAATAACGACAAAAATAAAATTGGCCGCTTCGGTCAGGGCCAACTATTGCCTGAAAAAGGGTAACGATATGAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAgg                                                                                                                                           >  2:1567248/1‑151 (MQ=255)
      cGCATCAGGCAATGAATGCGAAACCGCGGTGTAAATAACGACAAAAATAAAATTGGCCGCTTCGGTCAGGGCCAACTATTGCCTGAAAAAGGGTAACGATATGAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGc                                                                                                                                          <  5:188323/151‑1 (MQ=255)
       gCATCAGGCAATGAATGCGAAACCGCGGTGTAAATAACGACAAAAATAAAATTGGCCGCTTCGGTCAGGGCCAACTATTGCCTGAAAAAGGGTAACGATATGAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCg                                                                                                                                         >  1:243685/1‑151 (MQ=255)
       gCATCAGGCAATGAATGCGAAACCGCGGTGTAAATAACGACAAAAATAAAATTAGCCGCTTCGGTCAGGGCCAACTATTGCCTGAAAAAGGGTAACGATATGAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCg                                                                                                                                         <  4:902523/151‑1 (MQ=255)
         aTCAGGCAATGAATGCGAAACCGCGGTGTAAATAACGACAAAAATAAAATTGGCCGCTTCGGTCAGGGCCAACTATTGCCTGAAAAAGGGTAACGATATGAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCga                                                                                                                                        >  6:340382/1‑150 (MQ=255)
            aGGCAATGAATGCGAAACCGCGGTGTAAATAACGACAAAAATAAAATTGGCCGCTTCGGTCAGGGCCAACTATTGCCTGAAAAAGGGTAACGATATGAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCgaaga                                                                                                                                     >  8:311773/1‑150 (MQ=255)
             ggCAATGAATGCGAAACCGCGGTGTAAATAACGACAAAAATAAAATTGGCCGCTTCGGTCAGGGCCAACTATTGCCTGAAAAAGGGTAACGATATGAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCGAAGAAc                                                                                                                                   >  5:342293/1‑151 (MQ=255)
              gCAATGAATGCGAAACCGCGGTGTAAATAACGACAAAAATAAAATTGGCCGCTTCGGTCAGGGCCAACTATTGCCTGAAAAAGGGTAACGATATGAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCGAAGAACt                                                                                                                                  <  4:223040/151‑1 (MQ=255)
              gCAATGAATGCGAAACCGCGGTGTAAATAACGACAAAAATAAAATTGGCCGCTTCGGTCAGGGCCAACTATTGCCTGAAAAAGGGTAACGATATGAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCGAAGAACt                                                                                                                                  >  5:59204/1‑151 (MQ=255)
              gCAATGAATGCGAAACCGCGGTGTAAATAACGACAAAAATAAAATTGGCCGCTTCGGTCAGGGCCAACTATTGCCTGAAAAAGGGTAACGATATGAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCGAAGAACt                                                                                                                                  <  7:1680991/151‑1 (MQ=255)
                 atgaatgCGAAACCGCGGTGTAAATAACGACAAAAATAAAATTGGCCGCTTCGGTCAGGGCCAACTATTGCCTGAAAAAGGGTAACGATATGAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCGAAGAACtgaa                                                                                                                               <  1:1137246/151‑1 (MQ=255)
                 atgaatgCGAAACCGCGGTGTAAATAACGACAAAAATAAAATTGGCCGCTTCGGTCAGGGCCAACTATTGCCTGAAAAAGGGTAACGATATGAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCGAAGAACtgaa                                                                                                                               <  7:1195613/151‑1 (MQ=255)
                   gaatgCGAAACCGCGGTGTAAATAACGACAAAAATAAAATTGGCCGCTTCGGTCAGGGCCAACTATTGCCTGAAAAAGGGTAACGATATGAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCGAAGAACtgaatg                                                                                                                             <  3:94228/151‑1 (MQ=255)
                    aatgCGAAACCGCGGTGTAAATAACGACAAAAATAAAATTGGCCGCTTCGGTCAGGGCCAACTATTGCCTGAAAAAGGGTAACGATATGAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCGAAGAACtgaatg                                                                                                                             <  3:85292/150‑1 (MQ=255)
                       gCGAAACCGCGGTGTAAATAACGACAAAAATAAAATTGGCCGCTTCGGTCAGGGCCAACTATTGCCTGAAAAAGGGTAACGATATGAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCGAAGAACTGAATGAAg                                                                                                                          <  3:1306454/150‑1 (MQ=255)
                         gAAACCGCGGTGTAAATAACGACAAAAATAAAATTGGCCGCTTCGGTCAGGGCCAACTATTGCCTGAAAAAGGGTAACGATATGAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCGAAGAACTGAATGAAGat                                                                                                                        >  6:407197/1‑150 (MQ=255)
                           aaCCGCGGTGTAAATAACGACAAAAATAAAATTGGCCGCTTCGGTCAGGGCCAACTATTGCCTGAAAAAGGGTAACGATATGAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATAtt                                                                                                                     <  8:568621/151‑1 (MQ=255)
                             ccGCGGTGTAAATAACGACAAAAATAAAATTGGCCGCTTCGGTCAGGGCCAACTATTGCCTGAAAAAGGGTAACGATATGAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGGAAATTAGGCGAAGAACTGAATGAAGATATTGc                                                                                                                   >  2:1461889/1‑151 (MQ=255)
                               gcgGTGTAAATAACGACAAAAATAAAATTGGCCGCTTCGGTCAGGGCCAACTATTGCCTGAAAAAGGGTAACGATATGAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCt                                                                                                                 >  6:757111/1‑151 (MQ=255)
                               gcgGTGTAAATAACGACAAAAATAAAATTGGCCGCTTCGGTCAGGGCCAACTATTGCCTGAAAAAGGGTAACGATATGAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCGAAGAACAGAATGAAGATATTGCCt                                                                                                                 >  4:1589024/1‑151 (MQ=255)
                                cgGTGTAAATAACGACAAAAATAAAATTGGCCGCTTCGGTCAGGGCCAACTATTGCCTGAAAAAGGGTAACGATATGAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTg                                                                                                                >  6:895179/1‑151 (MQ=255)
                                 ggTGTAAATAACGACAAAAATAAAATTGGCCGCTTCGGTCAGGGCCAACTATTGCCTGAAAAAGGGTAACGATATGAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTgg                                                                                                               <  5:1331862/151‑1 (MQ=255)
                                    gtAAATAACGACAAAAATAAAATTGGCCGCTTCGGTCAGGGCCAACTATTGCCTGAAAAAGGGTAACGATATGAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGgcgc                                                                                                            >  1:796115/1‑151 (MQ=255)
                                       aaTAACGACAAAAATAAAATTGGCCGCTTCGGTCAGGGCCAACTATTGCCTGAAAAAGGGTAACGATATGAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCAtt                                                                                                         >  5:54844/1‑151 (MQ=255)
                                       aaTAACGACAAAAATAAAATTGGCCGCTTCGGTCAGGGCCAACTATTGCCTGAAAAAGGGTAACGATATGAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCAtt                                                                                                         >  3:463928/1‑151 (MQ=255)
                                           aCGACAAAAATAAAATTGGCCGCTTCGGTCAGGGCCAACTATTGCCTGAAAAAGGGTAACGATATGAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCATTGGTc                                                                                                     <  3:282488/151‑1 (MQ=255)
                                           aCGACAAAAATAAAATTGGCCGCTTCGGTCAGGGCCAACTATTGCCTGAAAAAGGGTAACGATATGAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCATTGGTc                                                                                                     <  5:1776270/151‑1 (MQ=255)
                                                 aaaataaaatTGGCCGCTTCGGTCAGGGCCAACTATTGCCTGAAAAAGGGTAACGATATGAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCATTGGTCGCGCCt                                                                                               <  7:7270/151‑1 (MQ=255)
                                                  aaataaaatTGGCCTCTTCGGTCAGGGCCAACTATTGCCTGAAAAAGGGTAACGATATGAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCATTGGTCGCGCCTa                                                                                              <  1:755039/151‑1 (MQ=255)
                                                  aaataaaatTGGCCGCTTCGGTCTGGGCCAACTATTGCCTGAAAAAGGGTAACGATATGAATAAATTATCGTGCTTTAAAGCCTATTATATTCTCGGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCATTGGTCGCGCCTa                                                                                              <  7:841936/151‑1 (MQ=255)
                                                   aataaaatTGGCCGCTTCGGTCAGGGCCGACTATTGCCTGAAAAAGGGTAACGATATGAAACAATTAAGCTGCTGTAAAGCCTATGATATTCGCGGAAAATTAGGCGAAGCACTGAATGAAGATATTGCCTGGCGCATTGGTCGCGCGTAt                                                                                             >  2:258882/1‑151 (MQ=255)
                                                      aaaatTGGCCGCTTCGGTCAGGGCCAACTATTGCCTGAAAAAGGGTAACGATATGAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCATTGGTCGCGCCTATgg                                                                                           <  2:1376151/150‑1 (MQ=255)
                                                        aatTGGCCGCTTCGGTCAGGGCCAACTATTGCCTGAAAAAGGGTAACGATATGAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCATTGGTCGCGCCTATGGCg                                                                                         >  5:1601325/1‑150 (MQ=255)
                                                           tGGCCGCTTCGGTCAGGGCCAACTATTGCCTGAAAAAGGGTAACGATATGAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCATTGGTCGCGCCTATGGCGAAt                                                                                      <  6:1809913/150‑1 (MQ=255)
                                                            ggCCGCTTCGGTCAGGGCCAACTATTGCCTGAAAAAGGGTAACGATATGAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGGAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCATTGGTCGCGCCTATGGCGAAttt                                                                                    <  1:1764709/151‑1 (MQ=255)
                                                              ccGCTTCGGTCAGGGCCAACTATTGCCTGAAAAAGGGTAACGATATGAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCATTGGTCGCGCCTATGGCGAATTtct                                                                                  >  4:703354/1‑151 (MQ=255)
                                                               cGCTTCGGTCAGGGCCAACTATTGCCTGAAAAAGGGTAACGATATGAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCATTGGTCGCGCCTATGGCGAATTtctc                                                                                 >  1:1483741/1‑151 (MQ=255)
                                                                gCTTCGGTCAGGGCCAACTATTGCCTGAAAAAGGGTAACGATATGAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCATTGGTCGCGCCTATGGCGAATTtctc                                                                                 >  1:454949/1‑150 (MQ=255)
                                                                gCTTCGGTCAGGGCCAACTATTGCCTGAAAAAGGGTAACGATATGAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCATTGGTCGCGCCTATGGCGAATTtctc                                                                                 >  7:1296517/1‑150 (MQ=255)
                                                                    cGGTCAGGGCCAACTATTGCCTGAAAAAGGGTAACGATATGAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCATTGGTCGCGCCTATGGCGAATTTCTCAAAcc                                                                            >  5:868447/1‑151 (MQ=255)
                                                                    cGGTCAGGGCCAACTATTGCCTGAAAAAGGGTAACGATATGAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCATTGGTCGCGCCTATGGCGAATTTCTCAAAcc                                                                            <  5:1530074/151‑1 (MQ=255)
                                                                    cGGTCAGGGCCAACTATTGCCTGAAAAAGGGTAACGATATGAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCATTGGTCGCGCCTATGGCGAATTTCTCAAAc                                                                             <  6:171353/150‑1 (MQ=255)
                                                                        cAGGGCCAACTATTGCCTGAAAAAGGGTAACGATATGAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCATTGGTCGCGCCTATGGCGAATTTCTCAAACCGaa                                                                         >  7:1042513/1‑150 (MQ=255)
                                                                          gggCCAACTATTGCCTGAAAAAGGGTAACGATATGAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCATTGGTCGCGCCTATGGCGAATTTCTCAAACCGAAAAc                                                                      <  5:1432177/151‑1 (MQ=255)
                                                                            gCCAACTATTGCCTGAAAAAGGGTAACGATATGAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCATTGGTCGCGCCTATGGCGAATTTCTCAAACCGAAAAcc                                                                     >  4:271033/1‑150 (MQ=255)
                                                                             ccAACTATTGCCTGAAAAAGGGTAACGATATGAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCATTGGTCGCGCCTATGGCGAATTTCTCAAACCGAAAACCAt                                                                   >  2:918267/1‑151 (MQ=255)
                                                                             ccAACTATTGCCTGAAAAAGGGTAACGATATGAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCATTGGTCGCGCCTATGGCGAATTTCTCAAACCGAAAACCAt                                                                   >  4:563007/1‑151 (MQ=255)
                                                                              cAACTATTGCCTGAAAAAGGGTAACGATATGAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCATTGGTCGCGCCTATGGCGAATTTCTCAAACCGAAAACCAtt                                                                  <  8:772528/151‑1 (MQ=255)
                                                                                  tATTGCCTGAAAAAGGGTAACGATATGAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCATTGGTCGCGCCTATGGCGAATTTCTCAAACCGAAAACCATTgtgt                                                              >  6:1480752/1‑151 (MQ=255)
                                                                                    ttGCCTGAAAAAGGGTAACGATATGAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCATTGGTCGCGCCTATGGCGAATTTCTCAAACCGAAAACCATTGTGtt                                                             >  8:1451890/1‑150 (MQ=255)
                                                                                    ttGCCTGAAAAAGGGTAACGATATGAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCATTGGTCGCGCCTATGGCGAATTTCTCAAACCGAAAACCATTGTGtt                                                             >  7:1762193/1‑150 (MQ=255)
                                                                                     tGCCTGAAAAAGGGTAACGATATGAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCATTGGTCGCGCCTATGGCGAATTTCTCAAACCGAAAACCATTGTGTTa                                                            <  8:1088009/150‑1 (MQ=255)
                                                                                     ggcttaaaaaaGGGTAACGATATGAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCATTGGTCGCGCCTATGGCGAATTTCTCAAACCGAAAACCATTGTGTTAg                                                           <  7:1932282/145‑1 (MQ=255)
                                                                                        cTGAAAAAGGGTAACGATATGAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCATTGGTCGCGCCTATGGCGAATTTCTCAAACCGAAAACCATTGTGTTAGGCg                                                        >  6:1149966/1‑151 (MQ=255)
                                                                                        cTGAAAAAGGGTAACGATATGAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCATTGGTCGCGCCTATGGCGAATTTCTCAAACCGAAAACCATTGTGTTAGGCg                                                        >  6:511432/1‑151 (MQ=255)
                                                                                         tGAAAAAGGGTAACGATATGAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCATTGGTCGCGCCTATGGCGAATTTCTCAAACCGAAAACCATTGTGTTAGGCgg                                                       >  3:1429328/1‑151 (MQ=255)
                                                                                          gAAAAAGGGTAACGATATGAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCATTGGTCGCGCCTATGGCGAATTTCTCAAACCGAAAACCATTGTGTTAGGCGGt                                                      >  6:137307/1‑151 (MQ=255)
                                                                                           aaaaaGGGTAACGATATGAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCATTGGTCGCGCCTATGGCGAATTTCTCAAACCGAAAACCATTGTGTTAGGCGGTg                                                     >  5:931781/1‑151 (MQ=255)
                                                                                           aaaaaGGGTAACGATATGAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCATTGGTCGCGCCTATGGCGAATTTCTCAAACCGAAAACCATTGTGTTAGGCGGTg                                                     >  2:1935078/1‑151 (MQ=255)
                                                                                           aaaaaCGGTAACGATATGAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGGAAATTAGGCGAAGTACTGAATGAAGATATTGCCTGGCGCATTGGTCGCGCCTATGGCGAATTTCTCAAACCGAAAACCATTGTGTTAGGCGGt                                                      <  2:686504/150‑1 (MQ=255)
                                                                                             aaaGGGTAACGATATGAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCATTGGTCGCGCCTATGGCGAATTTCTCAAACCGAAAACCATTGTGTTAGGCGGTGAt                                                   >  1:1634642/1‑151 (MQ=255)
                                                                                                gggTAACGATATGAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCATTGGTCGCGCCTATGGCGAATTTCTCAAACCGAAAACCATTGTGTTAGGCGGTGATGTc                                                >  8:1330558/1‑151 (MQ=255)
                                                                                                 ggTAACGATATGAAAAAATTAACCTGCTTTCAAGCCTATGATATTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCATTGGTCGCGCCTATGGCGAATTTCTCAAACCGAAAACCATTGTGTTAGGCGGTGATGTcc                                               <  6:1923947/151‑1 (MQ=255)
                                                                                                 ggTAACGATATGAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCATTGGTCGCGCCTATGGCGAATTTCTCAAACCGAAAACCATTGTGTTAGGCGGTGATGTcc                                               >  1:352336/1‑151 (MQ=255)
                                                                                                    aaCGATATGAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCATTGGTCGCGCCTATGGCGAATTTCTCAAACCGAAAACCATTGTGTTAGGCGGTGATGTCCGcc                                            >  1:936933/1‑151 (MQ=255)
                                                                                                    aaCGATATGAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCATTGGTCGCGCCTATGGCGAATTTCTCAAACCGAAAACCATTGTGTTAGGCGGTGATGTCCGc                                             <  2:21100/150‑1 (MQ=255)
                                                                                                      cGATATGAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCATTGGTCGCGCCTATGGCGAATTTCTCAAACCGAAAACCATTGTGTTAGGCGGTGATGTCCGCCTc                                          <  6:1237170/151‑1 (MQ=255)
                                                                                                        atatGAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGGAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCATTGGTCGCGCCTATGGCGAATTTCTCAAACCGAAAACCATTGTGTTAGGCGGTGATGTCCGCCTCAc                                        >  7:1453353/1‑151 (MQ=255)
                                                                                                        atatGAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCATTGGTCGCGCCTATGGCGAATTTCTCAAACCGAAAACCATTGTGTTAGGCGGTGATGTCCGCCTCAc                                        >  4:1203563/1‑151 (MQ=255)
                                                                                                        atatGAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCATTGGTCGCGCCTATGGCGAATTTCTCAAACCGAAAACCATTGTGTTAGGCGGTGATGTCCGCCTCAc                                        >  4:468729/1‑151 (MQ=255)
                                                                                                            gAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCATTGGTCGCGCCTATGGCGAATTTCTCAAACCGGAAACCATTGTGTTAGGCGGTGATGTCCGCCTCACCAGc                                    >  3:1869480/1‑151 (MQ=255)
                                                                                                            gAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCATTGGTCGCGCCTATGGCGAATTTCTCAAACCGAAAACCATTGTGTTAGGCGGTGATGTCCGCCTCACCAGc                                    >  1:681116/1‑151 (MQ=255)
                                                                                                             aaaaaaTTAACCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCATTGGTCGCGCCTATGGCGAATTTCTCAAACCGAAAACCATTGTGTTAGGCGGTGATGTCCGCCTCACCAGCg                                   <  4:1746097/151‑1 (MQ=255)
                                                                                                             aaaaaaTTAACCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCATTGGTCGCGCCTATGGCGAATTTCTCAAACCGAAAACCATTGTGTTAGGCGGTGATGTCCGCCTCACCAGCg                                   >  2:1278849/1‑151 (MQ=255)
                                                                                                              aaaaaTTAACCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCATTGGTCGCGCCTATGGCGAATTTCTCAAACCGAAAACCATTGTGTTAGGCGGTGATGTCCGCCTCACCAGCg                                   >  6:1078119/1‑150 (MQ=255)
                                                                                                               aaaaTTAACCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCATTGGTCGCGCCTATGGCGAATTTCTCAAACCGAAAACCATTGTGTTAGGCGGTGATGTCCGCCTCACCAGCGaa                                 <  3:1730612/151‑1 (MQ=255)
                                                                                                                 aaTTAACCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCATTGGTCGCGCCTATGGCGAATTTCTCAAACCGAAAACCATTGTGTTAGGCGGTGATGTCCGCCTCACCAGCGAAAc                               <  7:555143/151‑1 (MQ=255)
                                                                                                                  aTTAACCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCATTGGTCGCGCCTATGGCGAATTTCTCAAACCGAAAACCATTTTGTTAGGCGGGGATGTCCGCCACACAAGCGAAAc                               >  8:1799871/1‑150 (MQ=255)
                                                                                                                     aaCCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCATTGGTCGCGCCTATGGCGAATTTCTCAAACCGAAAACCATTGTGTTAGGCGGTGATGTCCGCCTCACCAGCGAAACCtt                            >  7:1545169/1‑150 (MQ=255)
                                                                                                                     aaCCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCATTGGTCGCGCCTATGGCGAATTTCTCAAACCGAAAACCATTGTGTTAGGCGGTGATGTCCGCCTCACCAGCGAAACCtt                            <  5:1074094/150‑1 (MQ=255)
                                                                                                                     aaCCTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCATTGGTCGCGCCTATGGCGAATTTCTCAAACCGAAAACCATTGTGTTAGGCGGTGATGTCCGCCTCACCAGCGAAACCTTa                           <  6:72330/151‑1 (MQ=255)
                                                                                                                        cTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCATTGGTCGCGCCTATGGCGAATTTCTCAAACCGAAAACCATTGTGTTAGGCGGTGATGTCCGCCTCACCAGCGAAACCTTaaa                         >  1:1277741/1‑150 (MQ=255)
                                                                                                                        cTGCTTTAAAGCCTATGATATTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCATTGGTCGCGCCTATGGCGAATTTCTCAAACCGAAAACCATTGTGTTAGGCGGTGATGTCCGCCTCACCAGCGAAACCTTaaa                         >  4:1287718/1‑150 (MQ=255)
                                                                                                                           cTTTAAAGCCTATGATATTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCATTGGTCGCGCCTATGGCGAATTTCTCAAACCGAAAACCATTGTGTTAGGCGGTGATGTCCGCCTCACCAGCGAAACCTTAAAActg                     >  3:603428/1‑151 (MQ=255)
                                                                                                                             ttAAAGCCTATGATATTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCATTGGTCGCGCCTATGGCGAATTTCTCAAACCGAAAACCATTGTGTTAGGCGGTGATGTCCGCCTCACCAGCGAAACCTTAAAActg                     <  4:1069063/149‑1 (MQ=255)
                                                                                                                               aaaGCCTATGATATTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCATTGGTCGCGCCTATGGCGAATTTCTCAAACCGAAAACCATTGTGTTAGGCGGTGATGTCCGCCTCACCAGCGAAACCTTAAAActggcgc                 >  7:956526/1‑151 (MQ=255)
                                                                                                                               aaaGCCTATGATATTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCATTGGTCGCGCCTATGGCGAATTTCTCAAACCGAAAACCATTGTGTTAGGCGGTGATGTCCGCCTCACCAGCGAAACCTTAAAActggcg                  <  5:1170891/150‑1 (MQ=255)
                                                                                                                                aaGCCTATGATATTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCATTGGTCGCGCCTATGGCGAATTTCTCAAACCGAAAACCATTGTGTTAGGCGGTGATGTCCGCCTCACCAGCGAAACCTTAAAActggcgct                <  2:1618446/151‑1 (MQ=255)
                                                                                                                                aaGCCTATGATATTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCATTGGTCGCGCCTATGGCGAATTTCTCAAACCGAAAACCATTGTGTTAGGCGGTGATGTCCGCCTCACCAGCGAAACCTTAAAActggcgct                <  3:1025917/151‑1 (MQ=255)
                                                                                                                                   ccTTTGATATTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCATTGGTCGCGCCTATGGCGAATTTCTCAAACCGAAAACCATTGTGTTAGGCGGTGATGTCCGCCTCACCAGCGAAACCTTAAAActggcgctggc             >  2:792660/1‑151 (MQ=255)
                                                                                                                                     tATGATATTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCGTTGGTCGCGCCTATGGCGAATTTCTCAAACCGAAAACCATTGTGTTAGGCGGTGATGTCCGCCTCACCAGCGAAACCTTAAAActggcgctggcg            >  7:366755/1‑150 (MQ=255)
                                                                                                                                     tATGATATTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCATTGGTCGCGCCTATGGCGAATTTCTCAAACCGAAAACCATTGTGTTAGGCGGTGATGTCCGCCTCACCAGCGAAACCTTAAAActggcgctggcg            >  8:1507262/1‑150 (MQ=255)
                                                                                                                                      aTGATATTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCATTGGTCGCGCCTATGGCGAATTTCTCAAACCGAAAACCATTGTGTTAGGCGGTGATGTCCGCCTCACCAGCGAAACCTTAAAACTGGCGCTGGCGa           >  2:1729655/1‑150 (MQ=255)
                                                                                                                                          tatTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCATTGGTCGCGCCTATGGCGAATTTCTCAAACCGCAAACCATTGTGTTAGGCGGTGATGTCCGCCTCACCAGCGAAACCTTAAAACTGGCGCTGGCGAAAGGt      >  3:730847/1‑151 (MQ=255)
                                                                                                                                           atTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCATTGGTCGCGCCTATGGCGAATTTCTCAAACCGAAAACCATTGTGTTAGGCGGTGATGTCCGCCTCACCAGCGAAACCTTAAAACTGGCGCTGGCGAAAGGtt     >  2:572942/1‑151 (MQ=255)
                                                                                                                                           atTCGCGGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCATTGGTCGCGCCTATGGCGAATTTCTCAAACCGAAAACCATTGTGTTAGGCGGTGATGTCCGCCTCACCAGCGAAACCTTAAAACTGGCGCTGGCGAAAGGtt     >  1:1022376/1‑151 (MQ=255)
                                                                                                                                              cgcgGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCATTGGTCGCGCCTATGGCGAATTTCTCAAACCGAAAACCATTGTGTTAGGCGGTGATGTCCGCCTCACCAGCGAAACCTTAAAACTGGCGCTGGCGAAAGGTTTAc  <  8:1210776/151‑1 (MQ=255)
                                                                                                                                               gcgGAAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCATTGGTCGCGCCTATGGCGAATTTCTCAAACCGAAAACCATTGTGTTAGGCGGTGATGTCCGCCTCACCAGCGAAACCTTAAAACTGGCGCTGGCGAAAGGTTTAc  >  3:1630647/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
AAACGTCGCATCAGGCAATGAATGCGAAACCGCGGTGTAAATAACGACAAAAATAAAATTGGCCGCTTCGGTCAGGGCCAACTATTGCCTGAAAAAGGGTAACGATATGAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGGAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCGCATTGGTCGCGCCTATGGCGAATTTCTCAAACCGAAAACCATTGTGTTAGGCGGTGATGTCCGCCTCACCAGCGAAACCTTAAAACTGGCGCTGGCGAAAGGTTTAC  >  DH1/1754971‑1755263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑