Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1613,6130TG24.5% 160.4 / 26.9 168intergenic (+474/+549)garK/yhaCglycerate 2‑kinase/pentapeptide repeats‑related protein
Reads supporting (aligned to +/- strand):  ref base T (47/73);  new base G (3/36);  total (52/116)
Fisher's exact test for biased strand distribution p-value = 1.26e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 170 total aligned reads displayed.

GGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑CGCTTCGGCGGGTTTTTGCTTTTGGA‑GGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTG  >  DH1/613463‑613725
                                                                                                                                                        |                                                                                                                
 gTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑CGCTTCGGCGGGTTTTTGCTTTTGGA‑GGGGCAGAAAGATGAATGCCTGTc                                                                                                                 >  7:773059/1‑151 (MQ=255)
  tAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGCCAGAACCCGGCTTATCGGTCAGTTTCACCTGCTTTACGTAAAAACC‑CGCGTCGGCGGCTTTTTGCTTTTGGA‑GGGGCAGAAAGATGAATGACTGTcc                                                                                                                >  5:237304/1‑151 (MQ=255)
   aGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑CGCTTCGGCGGGTTTTTGCTTTTGGA‑GGGGCAGAAAGATGAATGACTGTccc                                                                                                               >  7:835350/1‑150 (MQ=255)
   aGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACACAAACCGGCTAATACGTCAGTTTCACCTGATTTACGTAAAAACC‑ACAGTCGGCTGGATTTAGCTTTTGGA‑GGGGAACAAAGATGAATTACTGTcc                                                                                                                >  5:184279/1‑150 (MQ=25)
        gctTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑CGCTTCGGCGGGTTTTTGCTTTTGGA‑GGGGCAGAAAGATGAATGACTGTCCacgacg                                                                                                          <  4:482647/151‑1 (MQ=255)
        gctTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑CGCTTCGGCGGGTTTTTGCTTTTGGA‑GGGGCAGAAAGATGAATGACTGTCCacgac                                                                                                           <  6:1295420/150‑1 (MQ=255)
        gctTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACACGACATATCTGTAAGTATAACCTGATTTAAGTAAAAAAA‑AGCTTCGTCGGCTTATTAATTTTTAA‑ATAGCAGAAAGATGAATGACTGTCCacgagc                                                                                                          >  6:1091930/1‑149 (MQ=255)
            gAGCCATTTAGCTATTGCTGGCCTAGATGAATGACTGTCCCCGACATAACCCGGCTTATCGGTCAGTTTCACCTGATTTAGTTAAAAACC‑CGCTTCGGCGGTTTTTTGCTTTTGGA‑GGGGCATAAATATTAATGACTGTCCACGACGCtat                                                                                                      <  8:712747/151‑1 (MQ=18)
               ccAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑CGCTTCGGCGGGTTTTTGCTTTTGGA‑GGGGCAGAAAGATGAATGAGAGGCCAAGACCCtaaccc                                                                                                   >  5:375209/1‑147 (MQ=21)
                 aGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑CGCTTCGGCGGGTTTTTGCTTTTGGA‑GGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCa                                                                                                 <  8:465290/151‑1 (MQ=255)
                 aGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATAGGTCAGTTTCAGCTGATTTAAGTAAAAAAC‑ACCATAGGCTGGATTTTGGTTTTGGA‑GGGGCTGTAGGATGAATGACTGTCCCCGACGCTATAccc                                                                                                  >  7:721235/1‑150 (MQ=21)
                 aGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGAATAAAGGTCAGTTTCACCTGATTTACGTAAAAACC‑CCCTTCGGCGGGGTTTTGCTGTTGTA‑GGGGGAGAAAGATGAATGACTGTCCACGCCGCGATccccc                                                                                                 >  5:42557/1‑150 (MQ=21)
                  gTGAGCGTTTGGTGGCGTAGATTAATGACTGTCCACTACATAACCGGTCTTATCGGTCATTTTCACCTGATTTACGTAAAAACC‑CGCTTCGGGGGTTTTTTGATTTTGGT‑GGGGCATAAATATCAATGACTGTCCACGACGCTATACCCa                                                                                                 <  8:548882/150‑1 (MQ=17)
                  gTGAGCGTTGGCTGGCCTAGTTTAATTCCTGCCCACGACAGAACCCGTCTTAGGGGTCTTTTTCACCTTATTTACTTAAAAACC‑CGCTTGGGGGGTTTTTTGTTTTTGGT‑GGGGCACAAAGATTAATTACTGTCCACGACGCTATAccc                                                                                                  <  4:1455396/149‑1 (MQ=255)
                  gTGAGCGATGGCTGGCCTAGATGAATGACTGGCCACGACAGAACCCGGCTTATCGGGCAGTTTCACCTGATTTACGTAAAAACC‑CGCTTCGGCGGGTTTTTGCTTTTGGA‑GGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCaa                                                                                                <  5:688113/151‑1 (MQ=255)
                    gAGCGATTGCTGGCCTAGATCAATGACTGCCCACCACACAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAACCC‑CGCTTCGGCGGGTTTTTGCTTTTGGA‑GGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCaaaa                                                                                              <  4:186500/151‑1 (MQ=255)
                     aGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCTCCTTATTTACGTAAAAACA‑CGCTTCGGCGGGTTTTTGCTGTTGTT‑GCGGCAGAAAGATGAATTACTGTCCCCGACGCTATAcccgca                                                                                               >  1:521000/1‑146 (MQ=21)
                      gagATTGCTGGCCTAGTTGAATGACTGTCCACGACAGAACCCGTCTTATCGGTCATTTTCACGTGATTTACTTAAAAACC‑CGCTGCGGCGGTTTTTTGCTTTTGTA‑GGGGCAGAATGATGAATGACTGTCCACGACGCTATACCCAaaag                                                                                             <  2:1037415/148‑1 (MQ=255)
                      gCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑CGGGTCGGCGGGTTTTTGCTTTTGGA‑GGGGCAGAAAGATGAATGACTGTCCACGACCCTATACCCaaa                                                                                               >  1:1145740/1‑148 (MQ=255)
                        gATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATATACGTAAAAACC‑AGCTTCGTCGGGTTTTTGCTTTTGGA‑GGGTCATAAAGATGAATTAATGTCAAAGACTCTATACCCAaaagaa                                                                                           >  6:1404279/1‑150 (MQ=18)
                        gATTGCTGGCCTAGATGAATCACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑CGCTTCGGCGGGTTTTTGCTTTTGGA‑GGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAaaagaaa                                                                                          <  4:650740/151‑1 (MQ=255)
                         aTTGCTGGCCTAGATGAATGACTGCCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTTATTTACTTAAAAACC‑CGCTTCGGCGGGTTTTTGCTTTTGGA‑GGGGCAGAAAGATGAATGACTGGCCACGACGCTATACCCAaaagaaag                                                                                         <  7:592682/151‑1 (MQ=255)
                          ttGGTGGCGTAGATGAATGAGTGTCCACGACAGAACCCGGCTTATGGGTCAGTTTCACCTGATTTACGTAAAACCC‑GTCTTGGGCGGTTTTTTGCTTTTGGA‑GGGGCAGAAATATGATTGACTGTCCACGACGCTATACCCAaaagaaag                                                                                         <  3:1369666/150‑1 (MQ=17)
                           tGCTGGCCTTGATAAATGACTGTCCACCACAGAACCCTGATTATCGGTAAGTTTCACATGATTTACGTAAAAACG‑CGCTTGGGCGTGTTTTTGCTTTTGGC‑GCGGCCTAAACATCAATGACTGTCCACGACGCTATACCCAAAAGAAAGc                                                                                        <  3:815319/150‑1 (MQ=255)
                            ggtGGCCTAGATTATTCCCTGCCCACGACATAACCCGGGTTCGGGGGCATTTTCAGGTTTTTTCCGTAAAACCG‑CGTTTCGGGGGTTTTTTGCTTTTGGC‑GGGGCACAAATATGAATCCATGCCCACGACGCTATACCCAAAAGAAAGCg                                                                                       <  3:1448409/148‑1 (MQ=255)
                              tGGCCTAGATGAATGACTGTCCACGACACAAAACCCAAAATAAATCAGATACAAATCAATTACTTAAAAAAA‑AGCTTCTTAAGGTTTTTGATTATGTA‑GGTGCAGAAAGAATAATTAATATAAACGACACTATACCCAAAAGAAAGCggat                                                                                    >  6:828091/1‑149 (MQ=255)
                               ggcgttgATGAATGACTGTCCACGACAGAACCCGGCTTATGGGTGTGTTTAACCTGATTTACGTAAAAACC‑GGGTTCGGCGGGTTTTTGCTTTTGGA‑GGGGAAGAAAGATGAATGACTGGCCACGACGCTATACCCAAAAGAAAGCGGCtt                                                                                   <  1:326030/145‑1 (MQ=17)
                               ggCCTATATTAATCACTCCCCACGACAGAACCCGGCTTAGCGTTCAGTTTCACCTTATTTACTTAAAAACC‑CTCTTCGGCGGTTTTTTGATTTTTTA‑TGTTCTTAAATATCAATTACTGTCCACGACGCTATACCCAAAAGAAAGCGGCt                                                                                    <  4:1211963/150‑1 (MQ=12)
                               ggCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAATAACA‑AGCGTCGGCGGCTTATATCTAGTCTC‑GAGGCACAAACATGAATGACTGGGCAGGACGCTATAAAAAAAAGAAAGCGGCtt                                                                                   >  7:785839/1‑151 (MQ=16)
                               ggCCTAGATGAATGACTCTCCACCACAGAACCCGGCTTATCGGTCATTTTCACCTGATTTACTTAAAAACC‑ATTTTCGGCGGTTTTTTGCTTTTGTA‑TGTTCTGAAATATGAATTACTGTCCACGACGCTATACCCAAAAGAAAGCGGCt                                                                                    <  2:92958/150‑1 (MQ=17)
                               ggCATAGATTAATGACTGTCCACGACAGAACCCGGTTTATGGGTCTTTTTCACGTTATTTACGTAAAAACC‑GTCTTCGGGGGGTTTTTGCTTTTGTT‑GGGGCTTAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCtt                                                                                   <  2:951744/151‑1 (MQ=17)
                                 ccTAGATGAATGACTGCCCACGACAGAACCCGGCTTAGCGGTCAGTTTCACCTGATTTCCGTAAAACCC‑CGCTTCGGCGGGTTTTTGCTTTTGGA‑GGGGCACAAAGATGAATGACTGCCCACGACGCTATACCCAAAAGAAAGCGGCTTAt                                                                                 <  2:1356099/151‑1 (MQ=17)
                                  cTAGATGAATGACTGTCCACGACAGAAAACGCCTAATAGGTCAGTTTCACCTGATTTAAGTAAAAAAC‑CGCTTAGGAGGGTTTTTACTTTTGGA‑GGGGCAGAAAGATGAATTAATCTCCACGACACTATACCCAaaagaaaatcgcataac                                                                                >  8:641450/1‑141 (MQ=14)
                                    atattaatgaGTGTCCACGACAGAACCCGGCTTATCGGTCATTTTCACGTGATTTACTTAAAAACC‑CGGTTGGGGGTTTTTTTGATTTTGGA‑GGGGCACAAATATTAATGACTGCCCACGACGCTATACCCAAAAGAAAGCGGCTTATc                                                                                <  7:1053733/144‑1 (MQ=17)
                                    agttgaatgaCGGGCCCCGACAGAACCCGGCTTATCGGTCTGTTTCACCTGATTTACTTAAAAACC‑CGCTGCGGCGGTTTTTTGTTTTTGCT‑GGGCCAGAAAGTTGAATGACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCg                                                                               <  5:18841/147‑1 (MQ=14)
                                    aGATTAATGACTTTCCACGAAAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACTTAAAAACC‑CGCTTCGGCGGGTTTTTGCTTTTGGA‑GGGGCACAAAGATTAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCgg                                                                              <  8:226186/151‑1 (MQ=14)
                                      atgaatgaCTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTAACATAAATTAAGAAAAAACA‑CGATTCGAAGGGTTTTTACTTTAGTA‑TTGTCAGAAATATGAAACAATGTCATAAAAAAAATAACCAAAAGAAAGCGGCTGATCgggg                                                                            >  6:26790/1‑149 (MQ=9)
                                       cacgtgtcttaacccGACAGAACCCGGCTTATCGGCCAGTTTCACCTCTTTTACTTAAAAACC‑CGCTGCGGCGGGTTTTTGCTTTTGTC‑GGGGCACAAATATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCa                                                                           <  7:956214/137‑1 (MQ=11)
                                        gaatgaCTGCCCACCACAGAACCCGGCTTATCGGTCAGTTGCACCTGATTTCCGTAAAAACC‑CGCTTCGGGGGGTTTTTGCTTTTGGA‑GGGGCAGAAAGATGAATGACTGCCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCa                                                                           <  3:1402694/150‑1 (MQ=17)
                                         attgaATGTCCACGACAGACCCCGCCTTTGCTGGCATTTTCCCGTGATTTACGTAAAACCC‑GTCTGGGGCGGTTTTTTGGTTTTGTA‑GGGGCACAAAGTTTAATGACTGCCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAg                                                                          <  3:437779/148‑1 (MQ=9)
                                         aatgaCTTTCCTCTACATAACCCTGTTTATGGGTCATTTTCACCTGATTTACTTAAAAACC‑CGCTTCGGCGGGTTTTTGCTTTTGTA‑GGGGCTGAAATATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGt                                                                         <  2:306758/151‑1 (MQ=14)
                                         aatgaCTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAAGC‑AACTTCGGACGTGTTTTCATTATTGA‑GGGGCATCAAGATGGTTTACTGTCAACGACTCTATAAAAAAAAGTAAGCGGGTTATCGGTCAgg                                                                         >  7:192172/1‑150 (MQ=12)
                                         aatcaCTGCCCACGACAGACCCCGGCTTATGGGGCAGTTTCACCTGATTTACGTAAAAACC‑CGCTTCGGCGGGTTTTTGCTTTTGGA‑GGGGCACAAATATCAATGACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAg                                                                          <  1:1432095/150‑1 (MQ=14)
                                            gaCTGTCCAGTACAGAACCCGGGTTATGGGGAAGTTTCACCTGATTTACTTAAAACCG‑GGCTGCTGCGGGTTTTTGGTTTTGTA‑GGGGCAGAAAGATCAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTc                                                                      <  1:515006/151‑1 (MQ=14)
                                               tGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑CGCTTCGGCGGGTTTTTGCTTTTGGA‑GGGGCAGAAAGATGAATGACTGTcc                                                                                                                >  7:519941/1‑106 (MQ=255)
                                                ggccACGACACAACCGGGCTTATGGGACATTTGCCCCTGATTTACGAAAAACCC‑GGCTGCGGCGGTTTTTTGGTTTTGGC‑GGGGCACAAAGATGAATGAGGGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCAc                                                                    <  7:1313875/147‑1 (MQ=14)
                                                gaccaccaCACAACCCGGCTTATCGGCAAGTTGCACCTGATTTACGTAAAAACG‑CGCTTCGGCGGGTTTTTGCTTTTGCT‑GGGGCTCAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCt                                                                  <  5:1203699/149‑1 (MQ=14)
                                                gTCCACCACAGAACCCGGCTTATCGGTAATTTTCACGTGATTTAGTTAAAAACC‑TTCTTCGGCGGTTTTTTGTTTTTGTA‑GGTGATTAAATATGAATTACTTTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCa                                                                     <  6:1058781/148‑1 (MQ=14)
                                                  cgtctacacAACCGGGTTTATGGGGAATTTGCACGTGATTTTCTTAAACCCG‑GTCTTCTGCGGGTTTTTGTTTTTGTT‑GGGGTTTAATTATGATTGACTCGCCTATACGCTATACCCTAAAGAAAGCGGCTTATCGGTCAGTTTCACCTgg                                                                <  8:1438466/146‑1 (MQ=2)
                                                        cAGAACCCGGCTTATCGGTCAGTTTAACCTGAATAACGTAAAAACC‑ACCTTAAGCGAAATATTGATAATATA‑AGTGAAGAAATATGAATTACACTAAACAAAAAAAAAAAAAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTgg                                                                >  2:1088179/1‑145 (MQ=255)
                                                         aGAACCCGGGTTCTCGGGAAGTTTCGCGTGATTTCCTTAACACCG‑GGCTTCGGCGGGTTTTTGCTTTTGGA‑GGGGCTGAAAGATGAAGGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGt                                                         <  7:1363907/151‑1 (MQ=14)
                                                          caaCCCGGCTTATCGGGCAGTTGCACCTGATTTACTTAAAAACC‑CGCTGCGGCGGGTTTTTGCTTTTGCA‑GGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTa                                                        <  1:877957/150‑1 (MQ=255)
                                                             cccGGCTTATCGGGCAGTTTCCCCTGATTTACGTAAAACCC‑GGCTGCGGCGGTTTTTTGGTTTTGGA‑GGGGCTGAAACATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTaaaa                                                     <  5:1171807/151‑1 (MQ=11)
                                                             cccGGCTTAGCGGGCAGTTGCACATCATTT‑CCTAAAAACCGGGCTTCGGCGGGTTTTTGCTTTTGGA‑GGGGCCCAAAGATGAATGACTCGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTaaaa                                                     <  3:1328368/151‑1 (MQ=11)
                                                               cGGCTTATCGGTCATTATCACATGATTTACGTAAAAACA‑AACTTAGGATAGTTTTTGCTTTTGGA‑GGGTCAGAAAGATAAATGAATGTAAAAGACTCTATACCCAAAAGAAAGCGGCTTATCGGTCa                                                                           >  8:1162554/1‑127 (MQ=2)
                                                                    tagcGGTCATTTTCCCCTGATTAACGTAAAAAGC‑GGCTGCGGCGGGTTTTTGATTTTGTC‑GGGGCACAAATATGAATCCCTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCg                                                <  7:1421444/146‑1 (MQ=11)
                                                                    tATCGGTCAGTTACACCTGATTTACGTAAAAACA‑CGCTTCGGAGTGTTTTTGCTTTTGGA‑GGGGCATAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCAGCTGGAGAACGTaatctcgggat                                              >  8:513567/1‑142 (MQ=11)
                                                                    caTCGGCCACTTGCACCTGATTTCCGTAACCACC‑GGCTTCGGCGGGTTTTTGGTTTTGGT‑GGGGCCTAAATATGATTGCCTCTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCg                                                <  2:333471/148‑1 (MQ=11)
                                                                     aTCGGGAAGTTTCCCCTGATTTACGAAAAAACG‑CGCTGCGGCGGGTTTTTGCTTTTGGC‑GGGGCATAACGTTGAATCACTCGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCt                                              <  7:468791/150‑1 (MQ=11)
                                                                       ggggaaGTTGCACGTGATATACTAAAAACCC‑CGCTTCGGCGGGTTTTTGCTTTTGCC‑GGGGCACAAAGATGATTGCCTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTc                                            <  3:1103046/145‑1 (MQ=11)
                                                                       cGGGCAGTTTCACCTGATTTACGTAAACCCC‑CGCTGCGGCGGGTTTTTGCTTTTGGA‑GGGGCACAACGATGAATGACTGTCCACGACGCTATACCCAAAGGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTc                                            <  5:799910/150‑1 (MQ=11)
                                                                        ggTCATTTTCACGTCATTACCGTAACACCG‑CGCTTCGGCGGGTTTTTGCTTTTGGC‑GGCGCATAACGATGAATGCCTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTcgg                                          <  3:121688/151‑1 (MQ=11)
                                                                         gTCAGTTTCACCTGATTTACGTAAAAACC‑CGATTCGGCGGGTTTATGATTTTTGA‑GTGCCATAAATAAAAATAACTGAACACCAACCTATACCCAAAAAAAATATACTTATCGGTCACTATAACATGTTGTACTTaaaaaaaaaaaaaggc                                         >  4:577451/1‑140 (MQ=255)
                                                                          tCAGTTTCACCTGATTTACGTAAAAACC‑CGCTTCGGCGGGTTTTTGCTTTTGGA‑GGGGCAGATAGAGGAATGACTGTCCCCGACGCGATAGCCAAAAGAAAGCGGCTGATCGGTCAGTTTAACCTGCTGTACGTAATAACCCGCTTcggcg                                        >  3:25451/1‑151 (MQ=11)
                                                                          tCAGTTTCACCTGATTTACGTAAAAACC‑CGCTTCGGCGGGTTTTTGCTTTTGGA‑GGGGCAGAAAGATGAATGACTGTACACGACCCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTGTGACCTGGTGTACGTAATACCCCggtaggggg                                        >  5:1326989/1‑143 (MQ=11)
                                                                           cAGTTTCACCTGATTTACGTAAAAACC‑CGCTTCGGCGGCTTTTTGCTTTTGGA‑GGGGCAGAAAGATGAATGACAGTCCACGACGCTATAAACAAAAAAAAGAGTCATATCGGTCAGTTTCAGCTGTTTTACGTAAAAAcaggagggacgcg                                       >  8:407805/1‑139 (MQ=11)
                                                                           aattttctgttGATTTTCTTATAAACG‑CTCTGAGGCGGGTTTTTGTTTTTGTT‑GGGGTTTAATTTTGATTGACTTTACAAGAGGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTcggcgg                                       <  4:680388/141‑1 (MQ=2)
                                                                           aattttCACGTCATTTCCTTAAAAACC‑GTCTGCTGCGGGTTTTTGTTTTTGTA‑GGTGCACAACGATGAATGCCTCTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTcggcgg                                       <  2:778248/148‑1 (MQ=11)
                                                                            aGTTTCACCTGATTTACGTAAAAACC‑CGCTTCGGCGGGTTTTTTCTTTTGGA‑GGGGCAGAAAAAAGAATGACAGTCCTCGAGGGTAAAACAGAAAAAAATAGTCTTATAGTTCAGTTTCGCCTTATTTAATTAAAAAAAATCTTCGGCggg                                      >  4:617267/1‑151 (MQ=2)
                                                                                tCACCTGATTTACGTAAAACCC‑GGCTTCGGCGGGTTTTTGGTTTTGGC‑GGGGCACAACGATGAATGACTCTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGttt                                   <  1:492993/150‑1 (MQ=11)
                                                                                tCACCTGATTTACGTAAAAACC‑CGCTTCGGCGGGTTTTTGCTATTGGA‑GGGTCAGAAAGATCAATGACTGTCCACGACGCTATACCCAAAAGAAAGAGTCTTATCGGTCAGTTTGACCTGGTGTACGTAATAACCCGGTTCGGCGGTtttt                                  >  5:1283646/1‑151 (MQ=11)
                                                                                gcACCTCATTACCGAAAAACCC‑CGCTGCCGCGGGGATTGGCTTTTGGA‑GGGGCCGACCGAGGAATCACGCGCCACGACGCGATCCCCAACAGAACGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGtttt                                  <  6:529537/150‑1 (MQ=2)
                                                                                 cccccgatatccGTAAAAACC‑CGCTTCGGCGGGTTTTTGCTTTTGGC‑GGCGCAGAACGAGCACGGACTGCCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGttttt                                 <  7:379338/140‑1 (MQ=11)
                                                                                 cACCTGATTTACGTAAACACC‑GTCTTCGGCGGGTTTTTGGTTTTGGC‑GGGGCCCAACCATGAATGACTCTCCACGACTCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGtttt                                  <  1:820773/150‑1 (MQ=11)
                                                                                   ccTGATTTACGTAAAAACC‑CGCTTCGGCGGGTTTTTGCTTTTGGA‑GGGGCAGATAGCTGGAGGACAGGGCACGACGCGATTCACAAGAGAACGGGGCGTATGGGGGAGTGTCCGGTGGTTTACGTAAAACCCCGGGTGGGCGGGttttggg                               >  7:1148258/1‑148 (MQ=2)
                                                                                    cTGATTTACGTAAAAACC‑CGCTTCGGCGGGTTTTTGCTTTTGGA‑GGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCCGTCAGTTTCACCTGGTTTACGTAATAACCCGATGCGGCGGGTTtgtgat                              >  7:667934/1‑149 (MQ=11)
                                                                                     tGATTTACGTAAAAACC‑CGCTTCGGCGGGTTTTTGCTTTTGGA‑GGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGATTTACGTAAAAAACCGGTGCGGCGGGTTTGTGCtt                             >  1:1008280/1‑151 (MQ=11)
                                                                                       aTTTACGTAAAACCC‑CCGATCGGCGGGTTTTTGGTTTTGCC‑GGCGCACAAACATGAATGACTGGCCACGACGCTATACCCAAAAGAAACCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCtttt                           <  3:529972/151‑1 (MQ=11)
                                                                                         ttACGTAAAAACC‑CGCTTCGGCGGGTTTTTGCTTTTGGA‑GGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTGTACGTAAAAACCCGGTGCGGCGGGTTTGTGCTTGTgg                         >  3:255787/1‑151 (MQ=255)
                                                                                          accgaaaacaCC‑CGCTGCGGCGGGTTTTTGCTTTTGGA‑GCGGCACACCCATGAAGGCCTCGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGa                        <  5:491907/146‑1 (MQ=11)
                                                                                           acttAAAAACC‑GTCTTCTGCGGGTTTTTGTTTTTGTT‑GGTGCATAAATATGATTCCCGGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGa                        <  6:1305798/147‑1 (MQ=11)
                                                                                           aCGAAAAAACC‑CGCTTCGCCGGGTTTTTGCTTTTGCA‑GGGGCACAAACATGAATGACTCGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAg                       <  1:591850/151‑1 (MQ=11)
                                                                                            cGTAAAACCG‑CTTTTGGGCGGGTTTTTGTTTTTGGT‑GGTGCATAATTATGATTGACTTTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAgg                      <  2:461406/151‑1 (MQ=11)
                                                                                             gaaaccccc‑ccCTTCGCCGGGTTTTTGCTTTTGCC‑GGCGCCCAAACATGAATGACTCTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAggg                     <  5:750546/144‑1 (MQ=11)
                                                                                              tAAAAACG‑CTCTGCTGCGGGTTTTTGTTTTTGTT‑GGTGCACAATTATGATTGACTTTCGACGAGGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAg                       <  4:1240425/148‑1 (MQ=11)
                                                                                              gtaaacccggtctgcggcggGTTTTTGCTTTTGCC‑CGGGCCCAACCTTGAAGGACTGGCCACGACGCTCTACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAggg                     <  5:1423567/137‑1 (MQ=11)
                                                                                                aagacctgtcTTCTGCGGGTTTTTGTTTTTGGA‑GGTGCTCAATTTTTATTGAGTTTCCAATAGCCTATACCCAAAAGTAAGCGGCTTATAGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGc                   <  6:755564/142‑1 (MQ=2)
                                                                                                aacacccgcctgaggcggGTTTTTGGTGTTGCC‑GGCGCCCCAGAATGAAGGCCTCGCCACGACGCTATCCCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGCTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGc                   <  3:1364162/138‑1 (MQ=2)
                                                                                                   accgggCTTCTGCGGGTTTTTGTTTTTGTC‑GGTGGTTAATTATGATTGACTTTCCTCGTCGCTATACCCTAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGa                <  4:803854/146‑1 (MQ=11)
                                                                                                    cc‑cGCTGCGGCGGGTTTTGGCTTTTGCC‑GGCGCACAAGTATCATTCCCTCTCCACGACGCTATACCCTAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGaaa              <  7:188357/151‑1 (MQ=11)
                                                                                                        gCTTCGGCGGGTTTTTGCTTTTTGA‑GGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATGTACGTAAAAACCCGCTACGGCGGGttt                                   >  6:1090801/1‑127 (MQ=11)
                                                                                                         gtgaggcggGTTTTTGTTTTTGGT‑GGCGTATCACTATGATGGACTCGACAAGACTCTATACCCTAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAATAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGatg          <  1:981587/147‑1 (MQ=11)
                                                                                                          ttCGGCGGGTTTTTGCTTTTGGA‑GGGGCAGAAAGATGAATGACTGTCCACGAAGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGGTTTACGTAAAAAACCGGTTCGGCGGGTTTCTGCTTGTTGAgtgggctttagatct         >  1:280309/1‑140 (MQ=11)
                                                                                                            cgggggGTTTTTGTGTTTGGACGTGGCA‑TATGATCATTGACTGGCGACGACGGTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGatgaa        <  1:1379395/150‑1 (MQ=11)
                                                                                                             ggcggGTTTTTGCTTTTGCC‑CGGGTAAAACATTGATTGCCTCGCCACGACTCTCTACACAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGatgaatg      <  3:301280/151‑1 (MQ=11)
                                                                                                             cgcggGTTTTTGTTTTTGCC‑GGTGCCCACTGTTGATTGACTTGCCACGACGCTCTACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGatgaatg      <  3:1228844/150‑1 (MQ=11)
                                                                                                               cggGTTTTTGTTTTTGCT‑GGGGCCCTATCATGAATGACTCTCCACTACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGAc    <  4:759454/151‑1 (MQ=11)
                                                                                                                gggTTTTTGCTTTTGGA‑GGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAATAACCCGATGCGGCGGGTTTGAGCTTTTGGAGGGCCATaaataacaaactct   >  8:437173/1‑140 (MQ=11)
                                                                                                                 ggTTATTGTTTTTGTA‑GGGGCTTAAATATGATTGACTTTCTAATACTCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTg  <  8:1215362/151‑1 (MQ=11)
                                                                                                                                           aGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTc                                                                            >  5:1020355/1‑52 (MQ=35)
                                                                                                                                                        |                                                                                                                
GGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑CGCTTCGGCGGGTTTTTGCTTTTGGA‑GGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTG  >  DH1/613463‑613725

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑