Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1613,7700CA29.8% 185.6 / 18.4 145intergenic (+631/+392)garK/yhaCglycerate 2‑kinase/pentapeptide repeats‑related protein
Reads supporting (aligned to +/- strand):  ref base C (76/9);  new base A (36/0);  total (136/9)
Fisher's exact test for biased strand distribution p-value = 5.64e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 149 total aligned reads displayed.

AAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGTTTC  >  DH1/613635‑613919
                                                                                                                                         |                                                                                                                                                     
actgggcgtttgcGGTCATTTTCACTTGG‑TTACGTTAAGACCGGCCTGCGCCGGGTTTTTG‑TTTTTGAGGGCGGCCAAGATTGATGGCCTTGCCAAGACACTATACCCAAAAGAAACCGGCTTATCGGTCAGTTTCACCTGTTTTACGTaa                                                                                                                                        <  1:1043022/139‑1 (MQ=2)
     ggCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGATTTTGGAGGGGCAGAAAGA‑TGAATGAATGTAAACGACACTATAACCAAAACAAACAGGCTTATCGGTCAGTTTTACCTGATTTACGTaaaaaa                                                                                                                                    >  7:952245/1‑149 (MQ=11)
     ggCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTCTGATTTTCTAGGAAGAGAAAGC‑TGATTCACTCACGACACCACAATACAGAAAATAACGCGGCTTATCGTTGAGATAAACATGTTTTACGTATTAAcc                                                                                                                                   >  1:1213578/1‑151 (MQ=2)
         tATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCG‑GTTCGGCGGGTTTTTGATTTTGGAGTGGCATAAATA‑AGAAACACTTTCCACGACACAATAAACAAAAGAAAGATCCTAATAGGTCAGTTTAACCTGATTTACGTaaaaaaacggt                                                                                                                               >  4:1023760/1‑145 (MQ=255)
         tATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACGATACACAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGATTTACGtaataaacacat                                                                                                                               >  5:1402470/1‑145 (MQ=11)
          aTCGGTCAGTTTCACCTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGATAGA‑TGAAAGACTGTCCACGACACTATAACCAAATGAAAGCGGATAATCGGTCAGTTTGACATGTTTTaattaataaacaggtt                                                                                                                              >  4:654557/1‑136 (MQ=11)
           tCGGTCAGTTTCACCTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACCCggtcc                                                                                                                             >  1:1291740/1‑147 (MQ=25)
               tCAGTTTCACCTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGATTTACGTAAAAACCCGATgcggcg                                                                                                                         >  3:384895/1‑151 (MQ=255)
                 aGTTTCACCTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAAACCGTTGCGGCggg                                                                                                                       >  3:23318/1‑151 (MQ=255)
                 aGTTTCACCTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGATTTACGTAATAACCCGGTGCGGCggg                                                                                                                       >  5:910629/1‑151 (MQ=255)
                 aGTTTCACCTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATAAACAAAAGAAAGAGGCTTATCGGTCAGTTTAACCTGATTTACGTaaaaaacatcttaagagcg                                                                                                                       >  2:753626/1‑137 (MQ=11)
                  gTTTCACCTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAATAAACCGCTGCGGCGGGt                                                                                                                      >  5:742310/1‑151 (MQ=255)
                   tttCACCTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGATTATCGGTCACTTTGACCTGTTTTACGTAAAAAACCGATTCGGCGGGtt                                                                                                                     >  6:590200/1‑151 (MQ=255)
                     tCACCTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAACCCGATCCGGAGGGtttt                                                                                                                   >  4:1126527/1‑151 (MQ=255)
                      cACCTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGCAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAATAACCCGGTTCGGCGGGgtttt                                                                                                                  >  1:1351856/1‑151 (MQ=255)
                       aCCTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAAACCGATGCGGCGGGtttat                                                                                                                  >  8:77775/1‑148 (MQ=255)
                         cTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGATTTACGTAATAAACCGCTTCGGCGGGTTTTTGCt                                                                                                               >  1:135333/1‑151 (MQ=255)
                         cTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACCCGATACGGCGGGTTTATACt                                                                                                               >  2:491295/1‑151 (MQ=255)
                         cTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAACCCGATACGGCGGGTTTCTACt                                                                                                               >  4:1353762/1‑151 (MQ=255)
                         cTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAAACCGCTACGGCGGGtttttgat                                                                                                               >  4:501311/1‑148 (MQ=255)
                          tGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGATTTACGTAATAACCCGCTGCGGCGGGTTTGTGCtt                                                                                                              >  1:101978/1‑151 (MQ=11)
                          tGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCtt                                                                                                              >  1:3630/1‑151 (MQ=18)
                           ggTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTGCGGCGGGTTTTTACttt                                                                                                             >  7:1233548/1‑151 (MQ=37)
                           ggTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAAACCGCTACGGCGGGTTTATAAttt                                                                                                             >  8:1045486/1‑151 (MQ=11)
                            attaacccaaaacCCCG‑CTTCGGCGGTTTTTTTCTTTTTTAGTTTCATAAAGC‑TAAATGCCTGTCCACGACTCTATACACAAAAGAAAGCGTCTTATAGGTCATTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACtttt                                                                                                            <  6:1154019/143‑1 (MQ=2)
                             tttACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGGTAGCGGCTGAGCGGTGAGTTTCAGCCGGTTTACTTAAAAACCCGCGTCGGCGGGTTGTTACTTTTg                                                                                                           >  3:295847/1‑151 (MQ=11)
                              ttACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTAGAGCGGAAGCGCAGCGGGGTGTCGGGCAGTGACGCGTGTGGTTCGTAAAAGCCCGCTTCGGCGGGATTTTAATTTTgg                                                                                                          >  1:1385427/1‑151 (MQ=2)
                               tACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACCCGGTGCGGCGGGTTTTAGCTTTTgg                                                                                                          >  5:1130883/1‑150 (MQ=255)
                               tACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGA‑AGAATGACTGTCCACGACACTATACACAAAAGAAAGCGGCTTATCGGTCAGTTTAACCAGATTTACGAAAAAAACCGATACTGCTGGtttgaaatttttc                                                                                                          >  4:1337397/1‑140 (MQ=11)
                               tACGTAAAAACCCG‑CTTCGGCGGGTTTTTGATTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCCGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAAACCGCTACGGCGCGTTTATAAtttttg                                                                                                          >  8:877906/1‑148 (MQ=11)
                                aCGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGATTTACGTAAAAACCCGGTGCGGCGGGGTTGTCCTTTTgg                                                                                                          >  5:524957/1‑149 (MQ=11)
                                    aaaaaCCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAATAACCCGCTTCGGCGGGGTTGAGATTGTGGAggggg                                                                                                    >  5:451748/1‑150 (MQ=11)
                                    aaaaaCCCG‑CTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAAA‑ATAATAACAGACAACGAAACTGTAACCAAAAGAAAAAGACTTATCGAACAGTTTCACCACATATAAGTAAAAAAACGATTCGGCGGTGTTGTTCTTTTGGAggggg                                                                                                    >  4:966300/1‑150 (MQ=2)
                                          ccG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAAACCGGTGCGGCGGGTTTTAACTTTTTGAGGGCCatatat                                                                                              >  3:960148/1‑146 (MQ=11)
                                          ccG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACCCGATGCGGCGGGTTTTTGATTTTTGAGTGCGAGaaat                                                                                              >  1:720644/1‑150 (MQ=11)
                                                 cggcggGTTTTTGCTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAAACCCCTACGGCGGGTGTAAAATTTTGGAgtgcgcgaaatacaaa                                                                                         >  8:703514/1‑135 (MQ=11)
                                                               ttttGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAAACCGGTTCGGCGGGTTTTTACTTTTTGAGTGGGAGTAAGAGGAATGACTTTCCCCGac                                                                           >  7:1319803/1‑150 (MQ=11)
                                                                 ttGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAACCCGATTCGGCGGGTTTAAACTTTTGGAGTGGCAGAAAGATGTAACCCTTTCCCCGacac                                                                         >  4:1036814/1‑150 (MQ=11)
                                                                      ggggCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAAACCGATACGGCGGGTTTTTACTTTTGGAGTGGCATAAATAAGATACACTTTCGCCGACACtaaagc                                                                   >  8:719899/1‑147 (MQ=11)
                                                                                      atgaCTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAACCCGATACGGCGGGTTTTTAATTGTTGAGTGGGAGAAATTAGAAACACATTCCACGAGACTATAATCAaaagaaaccata                                                     >  8:856405/1‑145 (MQ=2)
                                                                                        gaCTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTGACCTTTTGGAGGGCGAGAAAGAGCAAAGCCTGGCGACGAGACGACACGAAAAAGAATGCGCCtt                                                   >  7:549660/1‑150 (MQ=11)
                                                                                          cTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGATTTACGTAAAAACCCGATACGGCGGGTTTGTAATTTTGGAGTGCGAGATATATGAAAGCCAGTCGACGACACGATCGCAAAATGAAACCAACTTATc                                                >  5:1023320/1‑151 (MQ=2)
                                                                                           tGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACCCGATACGGCGGGTTTATGATTTTTGAGTGCCAGAAATATCAAACACTTTCGACGTCACGATCGAAAAAAGAAAACAGCTTAACg                                               >  6:331311/1‑151 (MQ=2)
                                                                                           tGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTGCGGCGGGTTTTAACTTTTGGAGGGGCAGAAAGAGGAATGACTGTCCCCGACACTAGACGCAAAAGCAAGCGCCtgctcg                                               >  7:673709/1‑146 (MQ=11)
                                                                                           tGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACCCGCTACGGCGGGTTTTTACTTTTGGAGTGGCAGAAAGAAGAATGACTTTCGACGACACTATAACAAAAAGAAAGCGGCATATCg                                               >  6:824365/1‑151 (MQ=11)
                                                                                             tCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACCCGATACGGCGGGTTTTTACTTTTTGAGTGGCCTAAAGAAGAAACACTGTCCACGAGACGAAAAAAAAAAGAAAGCGCCTGATCgg                                              >  4:816214/1‑150 (MQ=11)
                                                                                              ccACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACCCGATTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAATATGAAAGACTGTCCCCGAGAATATCGACAAAAGAAAGCGGCTTAACgct                                             >  8:1461704/1‑148 (MQ=11)
                                                                                               cACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAACCCGCTACGGCGGGTTTATACTTTTGGAGGGGCAGAAAGAAGAAACAAAGTCCACGACAAGATAGAAAaaagaaaacaaaatatcgcaa                                            >  2:156112/1‑136 (MQ=11)
                                                                                                    cacTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAAACCGATGCGGCGGGTTTGTCCTTTTGGAGTGGCAGATAGATCAGAGACTGTCGCCGACACGATCCGCAAAAGACAGCGGCTTATCGGTCAGtgt                                       >  3:1216517/1‑148 (MQ=11)
                                                                                                       tataCCCCAAAGAAAGCGGATTATCGGTCAGTTTAACCTGTTTTACGTAAAAACCCGATTCGGCGGGTTTTTACTTTTGGAGGGCCACAAAGATGAATGACTGTCGACGAGACTATACACAAAAGAAAGCGCCTTATCGGGCactataac                                    >  2:1304088/1‑143 (MQ=255)
                                                                                                         taCCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAAAGACTGTCCACGACACTATACGCAAAAGAAAGCGGCTTATCGGTCAGTTTTACGTg                                 >  7:1214821/1‑151 (MQ=255)
                                                                                                            ccAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGCCTGTCCACGACACTATACCCAAAAGAAAGCTGCTTATCGGTCAGTATTACCTGATg                              >  1:452151/1‑151 (MQ=255)
                                                                                                            ccAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATg                              >  7:582747/1‑151 (MQ=38)
                                                                                                             cAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGAGGAAGGCCTGTCCACGTCACAGTAGCCAAAAGAAAGCGCCTGATCGCTCAGTTTTaagtgaggt                             >  3:845765/1‑143 (MQ=11)
                                                                                                             cAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGCCTTATCGGTCAGTTTTACCTGATGt                             >  8:484059/1‑151 (MQ=39)
                                                                                                             cAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTAACTTTTGGAGGGGCAGATAGATGATTGACTTTCCACGACACTATACAAAAAAGCAAGCGGCTTATCGCACATTTTTTCATGATGt                             >  3:183932/1‑151 (MQ=11)
                                                                                                              aaaaGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGTGGCAGAAAGAAGAATGACTGTCCACGACACTATACAAAAAAGAAAGCAGCTTATCGGTAATTATTACATGAtct                             >  8:400190/1‑148 (MQ=11)
                                                                                                              aaaaGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGt                             >  7:1232252/1‑150 (MQ=39)
                                                                                                              aaaaGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGAAGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTAACGGTCAGTTTTCCCTGATGt                             >  8:635653/1‑150 (MQ=255)
                                                                                                              aaaaGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGTGGCAGAAAGATGAAACACTTTCCACGACACTATACAAAAAAGAAAGCAGATTAACGCTAAGTTTTAAATGATGt                             >  2:1467348/1‑150 (MQ=11)
                                                                                                               aaagaaagCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACATGATGTAc                           >  7:161023/1‑151 (MQ=255)
                                                                                                               aaagaaagCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGGCCCCGACACTATACACAAAAGAAAGCGGATTAACGGTAAGTTTTACCTTATGTAc                           >  5:1003789/1‑151 (MQ=14)
                                                                                                               aaagaaagCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTGTACTTTTGGAGGGGCAGAAAGAAGAATGACTGTCCCCGTCACTATACCCAAAAGAAAGCGGCTGATCGGTCAGTTTTACCTGATGTAc                           >  3:432882/1‑151 (MQ=255)
                                                                                                                aagaaagCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGAGGAATGACAGTCCACGACACTATAGCCAAAAGAAAGCGGCTGATCGGTCAGTGTTACCTGATGTACg                          >  2:86902/1‑151 (MQ=14)
                                                                                                                aagaaagCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGGTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGAAGAGAGCCTGTCCCCGACACGAGACCCAAAAGAAAGCCCCTTATCGCTCAGTTTTACGTGATGTACg                          >  1:1129085/1‑151 (MQ=11)
                                                                                                                aagaaagCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACg                          >  1:1405863/1‑151 (MQ=40)
                                                                                                                 agaaagCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATCAATGACTGTCCACGAAACTATACACAAAAGAAAGAGCCATATCGGTCAGTTATACCTGATCTACg                          >  8:1459958/1‑150 (MQ=14)
                                                                                                                  gaacccGGCTTATCGGTCAGTTTCACCTGTTTTACGTAATAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCAc                                                                              <  5:314295/92‑1 (MQ=2)
                                                                                                                   aaagCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTTGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGACAGCGGCTTATCGGTCAGTTTTACCTGATGTACGta                        >  6:332058/1‑150 (MQ=255)
                                                                                                                   aaagCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGTGGCATAAATATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGATTATCGGTAATTTTTACATGATGTACGta                        >  4:81101/1‑150 (MQ=14)
                                                                                                                   aaagCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGCCTGTCCACGACACTATACCAAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATCTACGta                        >  3:1166463/1‑150 (MQ=255)
                                                                                                                    aagCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGtaat                      >  6:419255/1‑151 (MQ=40)
                                                                                                                    aagCGGCTTATCCGTCAGTTTCACCTGTTTTACGTAAAAACCCGATTCGGCGGGTTTTTACTTTTTCAGTGGAAGAAATATGAATGACTGTACACGACACAATACCAAAAACAAAGAGGAATATCCGTCATTTTTAACTGAGCTACGaaa                       >  8:1236203/1‑150 (MQ=255)
                                                                                                                        ggCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAAc                  >  1:892985/1‑151 (MQ=255)
                                                                                                                         gCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACGCAAAAGAAAGCGGCTTATCGCTCAGTTTTACATGATGTACGTAATAAAcc                 >  4:10165/1‑151 (MQ=255)
                                                                                                                         gCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAAcc                 >  2:953520/1‑151 (MQ=255)
                                                                                                                          cTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCg                >  3:604327/1‑151 (MQ=255)
                                                                                                                          cTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCg                >  6:983890/1‑151 (MQ=255)
                                                                                                                          cTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTACGGCGGGTTTTTGCTTTTTGAGTGGCAGAAAGATAAAACACTGTCCACGACACTATACACAAAAGAAAGCGCCTTATCGGAAAGTTTAAAAAGATGTACGTAATAAAc                  >  6:768783/1‑149 (MQ=11)
                                                                                                                           ttATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGt               >  1:467819/1‑151 (MQ=255)
                                                                                                                           ttATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCCGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGt               >  3:176721/1‑151 (MQ=255)
                                                                                                                            tATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGTGGCAGAAAGATGAATCACTGTCCACGACACTATAACCAAAAGAAAGCGGCTTATCGGACAGTATTAACTGATCTACGTAATAAAACGtt              >  6:484541/1‑151 (MQ=14)
                                                                                                                            tATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGtt              >  5:181300/1‑151 (MQ=255)
                                                                                                                            tATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATCACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGtt              >  4:487056/1‑151 (MQ=255)
                                                                                                                            gcttgttcctttgcACCTTTTTTACTTAAAAACCGGTTTGGGTTGTTTTTTTTTTTTGGCGGGGAAGAATTTTTAATTATTTTCCACGACTCTATACCCAAAAGAAAGCGGCTTTTCGGTCAGTTTTACCTGATGTACGTAATAAACCGtt              <  6:1327768/138‑1 (MQ=9)
                                                                                                                             ttttgtcaccttCACCTTTTTTACTTAAAAACCCGCTTCTGGTTTTTTTTTTTTTTTTTGTGGCATAATTATTATTGATTGTCTACGACTCTATATCCAAAAGAACTCGGCTTATCGGTCAGTTTTACCTGATTTACGTAATAAACCGtt              <  6:1284529/140‑1 (MQ=255)
                                                                                                                             aTCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTTCACGCCCCTATCCCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGAGGTACGTAATAAACCGTTc             >  6:880399/1‑151 (MQ=255)
                                                                                                                              tCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAATAAACCGttac            >  1:84532/1‑149 (MQ=9)
                                                                                                                               cGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCcg           >  6:590898/1‑151 (MQ=255)
                                                                                                                               cGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCcg           >  3:578907/1‑151 (MQ=255)
                                                                                                                               cGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCcg           >  6:1139327/1‑151 (MQ=255)
                                                                                                                                 gTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCcggc         >  8:1157752/1‑151 (MQ=255)
                                                                                                                                 gTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCcggc         >  4:1293338/1‑151 (MQ=255)
                                                                                                                                  tCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCcggcg        >  7:1138018/1‑151 (MQ=255)
                                                                                                                                   cAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTAAgaaagaaagagcttcgtctt       >  4:143722/1‑139 (MQ=11)
                                                                                                                                      tttCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGtt    >  7:678842/1‑151 (MQ=255)
                                                                                                                                       ttCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCGCGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGttt   >  7:1256828/1‑151 (MQ=255)
                                                                                                                                       ttCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGttt   >  4:60145/1‑151 (MQ=255)
                                                                                                                                        tCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGTTTc  >  2:1057832/1‑151 (MQ=255)
                                                                                                                                         cACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGTTTc  >  7:213554/1‑150 (MQ=255)
                                                                                                                                         |                                                                                                                                                     
AAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGTTTC  >  DH1/613635‑613919

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑