Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1613,7760TA24.5% 249.4 / 29.2 157intergenic (+637/+386)garK/yhaCglycerate 2‑kinase/pentapeptide repeats‑related protein
Reads supporting (aligned to +/- strand):  ref base T (104/10);  new base A (36/1);  total (146/11)
Fisher's exact test for biased strand distribution p-value = 2.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 160 total aligned reads displayed.

AAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACC‑CGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGTTTCAGATTGT  >  DH1/613635‑613926
                                                                                                                                               |                                                                                                                                                       
actgggcgtttgcGGTCATTTTCACTTGG‑TTACGTTAAGACCGGCCTGCGCCGGGTTTTTG‑TTTTTGAGGGCGGCCAAGATTGATGGCCTTGCCAAGACACTATACCCAAAAGAAACCGGCTTATCGGTCAGTTTCACCTGTTTTACGTaa                                                                                                                                                <  1:1043022/139‑1 (MQ=2)
     ggCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGATTTTGGAGGGGCAGAAAGA‑TGAATGAATGTAAACGACACTATAACCAAAACAAACAGGCTTATCGGTCAGTTTTACCTGATTTACGTaaaaaa                                                                                                                                            >  7:952245/1‑149 (MQ=11)
     ggCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTCTGATTTTCTAGGAAGAGAAAGC‑TGATTCACTCACGACACCACAATACAGAAAATAACGCGGCTTATCGTTGAGATAAACATGTTTTACGTATTAAcc                                                                                                                                           >  1:1213578/1‑151 (MQ=2)
         tATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCG‑GTTCGGCGGGTTTTTGATTTTGGAGTGGCATAAATA‑AGAAACACTTTCCACGACACAATAAACAAAAGAAAGATCCTAATAGGTCAGTTTAACCTGATTTACGTaaaaaaacggt                                                                                                                                       >  4:1023760/1‑145 (MQ=255)
         tATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACGATACACAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGATTTACGtaataaacacat                                                                                                                                       >  5:1402470/1‑145 (MQ=11)
          aTCGGTCAGTTTCACCTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGATAGA‑TGAAAGACTGTCCACGACACTATAACCAAATGAAAGCGGATAATCGGTCAGTTTGACATGTTTTaattaataaacaggtt                                                                                                                                      >  4:654557/1‑136 (MQ=11)
               tCAGTTTCACCTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGATTTACGTAAAAACC‑CGATgcggcg                                                                                                                                >  3:384895/1‑151 (MQ=255)
                 aGTTTCACCTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAAAC‑CGTTGCGGCggg                                                                                                                              >  3:23318/1‑151 (MQ=255)
                 aGTTTCACCTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGATTTACGTAATAACC‑CGGTGCGGCggg                                                                                                                              >  5:910629/1‑151 (MQ=255)
                 aGTTTCACCTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAAAC‑CGATGCGGCggg                                                                                                                              >  7:31993/1‑151 (MQ=255)
                 aGTTTCACCTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATAAACAAAAGAAAGAGGCTTATCGGTCAGTTTAACCTGATTTACGTaaaaaacatcttaagagcg                                                                                                                               >  2:753626/1‑137 (MQ=11)
                  gTTTCACCTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAATAAAC‑CGCTGCGGCGGGt                                                                                                                             >  5:742310/1‑151 (MQ=255)
                   tttCACCTGATTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGATGTTGGAAGCGAAGAAAGA‑TGAATGACTGTAAACGAAACTATACAAAAAAGAAAGCGGCTTATCATTAAATATAACCTGTTTTACGTAAAAACC‑CGCTTCGGCGGGtt                                                                                                                            >  8:705779/1‑151 (MQ=11)
                     tCACCTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAACC‑CGATCCGGAGGGtttt                                                                                                                          >  4:1126527/1‑151 (MQ=255)
                      cACCTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAAAC‑CGCTACGGCGGGtttt                                                                                                                          >  4:1024034/1‑150 (MQ=255)
                       aCCTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAAAC‑CGATGCGGCGGGtttat                                                                                                                         >  8:77775/1‑148 (MQ=255)
                         cTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGATTTACGTAATAAAC‑CGCTTCGGCGGGTTTTTGCt                                                                                                                      >  1:135333/1‑151 (MQ=255)
                         cTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAACC‑CGATACGGCGGGTTTCTACt                                                                                                                      >  4:1353762/1‑151 (MQ=255)
                          tGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGATTTACGTAATAACC‑CGCTGCGGCGGGTTTGTGCtt                                                                                                                     >  1:101978/1‑151 (MQ=11)
                           ggTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACC‑CGCTGCGGCGGGTTTTTACttt                                                                                                                    >  7:1233548/1‑151 (MQ=37)
                           ggTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAAAC‑CGCTACGGCGGGTTTATAAttt                                                                                                                    >  8:1045486/1‑151 (MQ=11)
                            attaacccaaaacCCCG‑CTTCGGCGGTTTTTTTCTTTTTTAGTTTCATAAAGC‑TAAATGCCTGTCCACGACTCTATACACAAAAGAAAGCGTCTTATAGGTCATTTTCACCTGTTTTACGTAAAAACC‑CGCTTCGGCGGGTTTTTACtttt                                                                                                                   <  6:1154019/143‑1 (MQ=2)
                              ttACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAAAC‑CGTTACGGCGGGttatatcttatg                                                                                                                  >  2:1372920/1‑140 (MQ=11)
                              ttACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACATGATTTACGTAAAAACC‑CGATTCGGCGGGtttaaaattgttg                                                                                                                 >  4:1337407/1‑141 (MQ=11)
                               tACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑CGATACGGCGGGTTTGTCATTTTgg                                                                                                                 >  6:411663/1‑150 (MQ=11)
                               tACGTAAAAACCCG‑CTTCGGCGGGTTTTTGATTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCCGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAAAC‑CGCTACGGCGCGTTTATAAtttttg                                                                                                                 >  8:877906/1‑148 (MQ=11)
                                ccctacaaaCCCG‑CAGCGGCGGGTTTTTGCTTTTGGAGGGCAAGAACCA‑TCAAGCACTGTCCACGACACTATACCAAAAACAAACCGGCTTATCGGGCACGTTCACCTGTTTTACGTAAAAACC‑CGCTTCGGCGGGTTTTTACTTTTGGAg                                                                                                               <  3:434774/145‑1 (MQ=11)
                                aCGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGATTTACGTAAAAACC‑CGGTGCGGCGGGGTTGTCCTTTTgg                                                                                                                 >  5:524957/1‑149 (MQ=11)
                                    aaaaaCCCG‑CTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAAA‑ATAATAACAGACAACGAAACTGTAACCAAAAGAAAAAGACTTATCGAACAGTTTCACCACATATAAGTAAAAAAA‑CGATTCGGCGGTGTTGTTCTTTTGGAggggg                                                                                                           >  4:966300/1‑150 (MQ=2)
                                        aCCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACC‑CGCTTCGGCGGGTTTATTCTTTTTGAgtggg                                                                                                           >  1:229762/1‑146 (MQ=255)
                                            g‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGATGTACGTAAAAACC‑CGCTGCGGCGGGTTTGTAATTGTGGAGTGGgagatata                                                                                                    >  4:1219096/1‑146 (MQ=11)
                                                tCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAAAC‑CGCTACGGCGGGTTTATAATTTTTGAGTGGCattaattaga                                                                                                 >  4:540617/1‑141 (MQ=11)
                                                 cggcggGTTTTTGCTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAAAC‑CCCTACGGCGGGTGTAAAATTTTGGAgtgcgcgaaatacaaa                                                                                                >  8:703514/1‑135 (MQ=11)
                                                                               aGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACC‑CGATACGGCGGGTTTATACTTTTTGAGTGGCAGATATAAGAAACACTTTCGACGACACTAGAAACAaaagaa                                                                  >  6:754906/1‑150 (MQ=11)
                                                                                      atgaCTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAAAC‑CGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGTAAGATGAATCACTGTAGCCGTCACTCTAGCCTAATGAAAGCGGCt                                                           >  1:1320282/1‑151 (MQ=11)
                                                                                        gaCTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACC‑CGCTTCGGCGGGTTTATACTTTTGGAGGGGCAGAAAGAGTAATGACTGTCCACGACACTATAAACAAAAGAAAGCCGCtg                                                          >  8:1304678/1‑149 (MQ=11)
                                                                                          cTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGATTTACGTAAAAACC‑CGATACGGCGGGTTTGTAATTTTGGAGTGCGAGATATATGAAAGCCAGTCGACGACACGATCGCAAAATGAAACCAACTTATc                                                       >  5:1023320/1‑151 (MQ=2)
                                                                                           tGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACC‑CGATACGGCGGGTTTATGATTTTTGAGTGCCAGAAATATCAAACACTTTCGACGTCACGATCGAAAAAAGAAAACAGCTTAACg                                                      >  6:331311/1‑151 (MQ=2)
                                                                                           tGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACC‑CGCTGCGGCGGGTTTTAACTTTTGGAGGGGCAGAAAGAGGAATGACTGTCCCCGACACTAGACGCAAAAGCAAGCGCCtgctcg                                                      >  7:673709/1‑146 (MQ=11)
                                                                                           tGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACC‑CGCTACGGCGGGTTTATACTTGTTGAGGGGGAGAAATAGGAGACACTTTCCCCGACACGGGAGGCAAAAGAAAGCTGATTATCg                                                      >  5:1068081/1‑151 (MQ=11)
                                                                                           tGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACC‑CGCTACGGCGGGTTTTTACTTTTGGAGTGGCAGAAAGAAGAATGACTTTCGACGACACTATAACAAAAAGAAAGCGGCATATCg                                                      >  6:824365/1‑151 (MQ=11)
                                                                                            gTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACC‑CGCTACGGCGGGTTTTTACTTTTGGAGGGCGATAAAGATGAATGACTGTCCCCGACACTAAACCCAAAAGAAAGCGGCATATCgg                                                     >  8:815587/1‑151 (MQ=11)
                                                                                            gTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAAAC‑CGATACGGCGGGTTTATAATTTTGGAGTGGCATAAATAAAAAACACTGACCAAGACACTATACAAAAAATAAAGCGGATTAtatt                                                     >  8:547243/1‑148 (MQ=2)
                                                                                               cACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAACC‑CGCTACGGCGGGTTTATACTTTTGGAGGGGCAGAAAGAAGAAACAAAGTCCACGACAAGATAGAAAaaagaaaacaaaatatcgcaa                                                   >  2:156112/1‑136 (MQ=11)
                                                                                                    cacTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAAAC‑CGATGCGGCGGGTTTGTCCTTTTGGAGTGGCAGATAGATCAGAGACTGTCGCCGACACGATCCGCAAAAGACAGCGGCTTATCGGTCAGtgt                                              >  3:1216517/1‑148 (MQ=11)
                                                                                                    cacTATACCCAAAAGAAAGCGGCTTAACGGTCAGTTTGACCTGTTTTACGTAAAAACC‑CGGTGCGGCGGGTTTGTGATTTTGGAGGGCCAGAAAGACGATGCCCTGGCGACGACACGAGACCAAAATGAAACCGGCTGAACGCACAGtta                                              >  3:763064/1‑149 (MQ=2)
                                                                                                       tataCCCCAAAGAAAGCGGATTATCGGTCAGTTTAACCTGTTTTACGTAAAAACC‑CGATTCGGCGGGTTTTTACTTTTGGAGGGCCACAAAGATGAATGACTGTCGACGAGACTATACACAAAAGAAAGCGCCTTATCGGGCactataac                                           >  2:1304088/1‑143 (MQ=255)
                                                                                                         taCCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACC‑CGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAAAGACTGTCCACGACACTATACGCAAAAGAAAGCGGCTTATCGGTCAGTTTTACGTg                                        >  7:1214821/1‑151 (MQ=255)
                                                                                                            ccAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACC‑CGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATg                                     >  7:582747/1‑151 (MQ=38)
                                                                                                             cAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACC‑CGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGAGGAAGGCCTGTCCACGTCACAGTAGCCAAAAGAAAGCGCCTGATCGCTCAGTTTTaagtgaggt                                    >  3:845765/1‑143 (MQ=11)
                                                                                                              aaaaGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACC‑CGCTTCGGCGGGTTTTTACTTTTGGAGTGGCAGAAAGAAGAATGACTGTCCACGACACTATACAAAAAAGAAAGCAGCTTATCGGTAATTATTACATGAtct                                    >  8:400190/1‑148 (MQ=11)
                                                                                                              aaaaGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACC‑CGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGt                                    >  7:1232252/1‑150 (MQ=39)
                                                                                                               aaagaaagCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACC‑CGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACATGATGTAc                                  >  7:161023/1‑151 (MQ=255)
                                                                                                               aaagaaagCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAAAC‑CGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAAAGACTGTCCACGCGACTAAACCCAAAAGCAAGCGGCATATCGCTCAGTTTTACCTGATGTa                                   >  1:457757/1‑150 (MQ=14)
                                                                                                                aagaaagCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACC‑CGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGAGGAATGACAGTCCACGACACTATAGCCAAAAGAAAGCGGCTGATCGGTCAGTGTTACCTGATGTACg                                 >  2:86902/1‑151 (MQ=14)
                                                                                                                aagaaagCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACC‑CGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACg                                 >  1:1405863/1‑151 (MQ=40)
                                                                                                                 agaaagCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACC‑CGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATCAATGACTGTCCACGAAACTATACACAAAAGAAAGAGCCATATCGGTCAGTTATACCTGATCTACg                                 >  8:1459958/1‑150 (MQ=14)
                                                                                                                  gaacccGGCTTATCGGTCAGTTTCACCTGTTTTACGTAATAACC‑CGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCAc                                                                                     <  5:314295/92‑1 (MQ=2)
                                                                                                                   aacgcgccggaTCGGGCAGTTTC‑CCGTTCTTACGTTACAACCGCCCTTCGGCGGGTTTTTGTGTTTGACGGCGCCCAATCATGAATGACTCGCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGtaa                              <  5:15989/141‑1 (MQ=2)
                                                                                                                   aaagCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACC‑CGCTTCGGCGGGTTTTTACTTTTGGAGTGGCATAAATATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGATTATCGGTAATTTTTACATGATGTACGta                               >  4:81101/1‑150 (MQ=14)
                                                                                                                   aaagCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACC‑CGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGCCTGTCCACGACACTATACCAAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATCTACGta                               >  3:1166463/1‑150 (MQ=255)
                                                                                                                   aaagCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACC‑CGATTCGGCGGGTTTTTACTTTTGGAGGGGGAGAAAGATGAATCACTTTCCACGACACTATACACAAAAGAAAGCCGCATAACGGTCAGATATAAATGATGTACGtat                              >  4:87861/1‑150 (MQ=14)
                                                                                                                    aagCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACC‑CGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGtaat                             >  6:419255/1‑151 (MQ=40)
                                                                                                                      gccgcttagggtccAGTTTCACCTGTTTTCCGTAAAAACC‑CGCTTCGGCGGTTTTTTTATTTTGGATGTCCAGAAATATCAATTACTTTCCACGACACTATACCCAAAAGAAAGCGGCTTATCTGTCAGTTTTACCTGATGTACGtaataa                           <  1:752104/138‑1 (MQ=14)
                                                                                                                        gggctctgcggctctttCACCTGATTTACGAAAAACCC‑CGCTTCGGCGGTTTTTTCTTTTTGGAGGGGCACAAAGATTAATGACTGGCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAAc                         <  7:159073/137‑1 (MQ=14)
                                                                                                                        ggCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACC‑CGCTTCGGCGGGTTTTTACTTTTTGAGGGGCAGAAAGATGAATGCCAGTCCACGACACAAAACACAAAAGAAACCGGATAATCGGTCAGTTTTACATGATGTAAGTAATAAAc                         >  2:1029402/1‑151 (MQ=14)
                                                                                                                         gCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACC‑CGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAAcc                        >  2:953520/1‑151 (MQ=255)
                                                                                                                         gCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACC‑CGCTTCGGCGGGTTTTTGATTTTGGAGTGGCAGAAAGATGAGGGACTTTCCGCGACAATATACAAAAAAGAAAGCGGATTAACGGTAAGTTTAAACTGATGTACTTAATAAAcc                        >  5:355420/1‑151 (MQ=11)
                                                                                                                          cTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACC‑CGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCg                       >  3:604327/1‑151 (MQ=255)
                                                                                                                          cTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACC‑CGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCg                       >  6:983890/1‑151 (MQ=255)
                                                                                                                           ttATCGGTCAGTTTCACCTGTTTTACGTAAAAACC‑CGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGt                      >  1:467819/1‑151 (MQ=255)
                                                                                                                           ttATCGGTCAGTTTCACCTGTTTTACGTAAAAACC‑CGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGt                      >  3:189495/1‑151 (MQ=255)
                                                                                                                           ttATCGGTCAGTTTCACCTGTTTTACGTAAAAACC‑CGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCCGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGt                      >  3:176721/1‑151 (MQ=255)
                                                                                                                            tATCGGTCAGTTTCACCTGTTTTACGTAAAAACC‑CGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATCACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGtt                     >  4:487056/1‑151 (MQ=255)
                                                                                                                            tATCGGTCAGTTTCACCTGTTTTACGTAAAAACC‑AGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATCACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAAACGtt                     >  6:589153/1‑151 (MQ=255)
                                                                                                                             ttttgtcaccttCACCTTTTTTACTTAAAAACC‑CGCTTCTGGTTTTTTTTTTTTTTTTTGTGGCATAATTATTATTGATTGTCTACGACTCTATATCCAAAAGAACTCGGCTTATCGGTCAGTTTTACCTGATTTACGTAATAAACCGtt                     <  6:1284529/140‑1 (MQ=255)
                                                                                                                              tCGGTCAGTTTCACCTGGTTTACGTAAAAACC‑CGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAATAAACCGttac                   >  1:84532/1‑149 (MQ=9)
                                                                                                                               cGGTCAGTTTCACCTGTTTTACGTAAAAACC‑CGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCcg                  >  6:1139327/1‑151 (MQ=255)
                                                                                                                               cGGTCAGTTTCACCTGTTTTACGTAAAAACC‑CGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCcg                  >  6:590898/1‑151 (MQ=255)
                                                                                                                               cGGTCAGTTTCACCTGTTTTACGTAAAAACC‑CGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCcg                  >  3:964819/1‑151 (MQ=255)
                                                                                                                                 gTCAGTTTCACCTGTTTTACGTAAAAGCC‑CGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCcggc                >  2:311744/1‑151 (MQ=255)
                                                                                                                                 gTCAGTTTCACCTGTTTTACGTAAAAACC‑CGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCcggc                >  4:1293338/1‑151 (MQ=255)
                                                                                                                                 gTCAGTTTCACCTGTTTTACGTAAAAACC‑CGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCcggc                >  8:1157752/1‑151 (MQ=255)
                                                                                                                                  tCAGTTTCACCTGTTTTACGTAAAAACC‑CGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCcggcg               >  7:1138018/1‑151 (MQ=255)
                                                                                                                                   tggtgtaaacTGTTTTGCGTTAACCCG‑CTCTTCGGCGGGTTTTTTCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCcggcg               <  5:1156102/141‑1 (MQ=17)
                                                                                                                                      tttCACCTGTTTTACGTAAAAACC‑CGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGtt           >  1:1130695/1‑151 (MQ=255)
                                                                                                                                      tttCACCTGTTTTACGTAAAAACC‑CGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGtt           >  7:678842/1‑151 (MQ=255)
                                                                                                                                       ttCACCTGTTTTACGTAAAAACC‑CGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCGCGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGttt          >  7:1256828/1‑151 (MQ=255)
                                                                                                                                       ttCACCTGTTTTACGTAAAAACC‑CGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGttt          >  6:832568/1‑151 (MQ=255)
                                                                                                                                       ttCACCTGTTTTACGTAAAAACC‑CGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGttt          >  4:60145/1‑151 (MQ=255)
                                                                                                                                       ttCACCTGATTTACGTAAAAACC‑CGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATTTACGTAATAAAcagatacgaagagcgt          >  8:159285/1‑136 (MQ=11)
                                                                                                                                       atCACGTGTTTTACGTAAAAACC‑CGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGtt           <  1:1368520/149‑1 (MQ=255)
                                                                                                                                         cACCTGTTTTACGTAAAAACC‑CGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGTTTc         >  6:1422981/1‑150 (MQ=255)
                                                                                                                                         cACCTGTTTTACGTAAAAACC‑CGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGTTTc         >  3:354409/1‑150 (MQ=255)
                                                                                                                                          aCCTGTTTTACGTAAAAACC‑CGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGTTTc         >  1:385637/1‑149 (MQ=255)
                                                                                                                                           ccTGTTTTACGTAAAAACC‑CGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGTTTc         >  4:681985/1‑148 (MQ=255)
                                                                                                                                           ccTGTTTTACGTAAAAACC‑CGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGTTTc         >  4:1466960/1‑148 (MQ=255)
                                                                                                                                            cTGTTTTACGTAAAAACC‑CGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGTTTc         >  3:62490/1‑147 (MQ=255)
                                                                                                                                            cTGATTTACGTAAAAACC‑CGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACCCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGTTGTACGTAAAAAACCGGTCCGGCGGGTTTCTGAt     >  7:39871/1‑151 (MQ=11)
                                                                                                                                               ttttACGTAAAAACC‑CGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGTTTCAGAttgt  >  4:389066/1‑151 (MQ=255)
                                                                                                                                               |                                                                                                                                                       
AAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACC‑CGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGTTTCAGATTGT  >  DH1/613635‑613926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑