Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1936,2781.T38.6% 112.7 / 145.3 70intergenic (‑12/+22)ECDH1_RS04620/ECDH1_RS04625tRNA‑Met/tRNA‑Met
Reads supporting (aligned to +/- strand):  ref base . (40/3);  new base T (22/5);  total (62/8)
Fisher's exact test for biased strand distribution p-value = 2.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.26e-02

TACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTT  >  DH1/936163‑936427
                                                                                                                    |                                                                                                                                                     
tACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCAGGATATGACCCCACGACCTTCGGCTTATCAGCCATACCAGCTACCATGCAGCTCCACCCCGCGTAAAAGTACTGCTTTAGTTCGTCAAATTTTAATTGGTTGCggggg                                                                                                                      >  1:452493/1‑149 (MQ=255)
  cTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTCCGTTACTTCATCAAATTTTAATTCGTTGcggcggaatt                                                                                                                  >  4:237835/1‑147 (MQ=255)
                                         ggATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGTAGGCCGTGACTTCAGCGAAGTATATTTCGTTCCGCGGGGCGGTTTTGAACCGACGGCATTGGGGtttttagtgcgcccagc                                                                           >  5:405707/1‑136 (MQ=2)
                                                    gacgacCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGACGTATTTCAACAGACGACCTTAGGCTTATGAGACCGACTAGCTACCAGgctg                                                                 >  8:1075308/1‑150 (MQ=14)
                                                                         ccGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCAGTGGATg                                           >  7:59620/1‑151 (MQ=255)
                                                                             cgaGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGcc                                                                                   <  1:1114550/107‑1 (MQ=255)
                                                                             cgaGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATgcgcc                                       >  8:1075931/1‑150 (MQ=255)
                                                                               aGCTACCAGGCTGCTCCACCCCGCGTCACGGTAGTGGTTTACTTCATCAATGTTTTATTGGTTCCCCGGGGCGGATATCACCCGACGACATTGGGCTTATGATACCGCAGAGCTACCAGGCTGCTCCCCACCGCGTcaggcgatggtcact                                     >  5:184006/1‑137 (MQ=2)
                                                                                 cTACCAGGCTGCTCCACCCCGCGTCACCGTAGTGGGTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGTTTTGAACCGCCGACCTTCGGGTTATGAGGCCGACGAGCTACCAGGCTGCTCCCCCCCGCGTCCGTGGATGGGCACtat                                   >  7:213334/1‑151 (MQ=17)
                                                                                         ctgctCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCCCTATACTCCGcg                           >  4:1069025/1‑150 (MQ=255)
                                                                                         ctgctCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCt                           >  8:605048/1‑151 (MQ=255)
                                                                                          tgctCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTCGTTGCGGGGGCCGGATTTGAACAGCCGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGGGCACTATACTCCGCTc                          >  7:699807/1‑151 (MQ=255)
                                                                                           gctCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGACGGATTTGAACCGACGACCTTAGGGATATGAGACCGAAAAGCTACCAGGCTACTCCACCCAGCGTCAGAGGATGCTCACTATACTACGCTcg                         >  4:1339855/1‑151 (MQ=17)
                                                                                             tCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTcgcg                       >  8:1309869/1‑151 (MQ=255)
                                                                                               cACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGtt                     >  6:1096693/1‑151 (MQ=255)
                                                                                               cACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGtt                     >  6:273952/1‑151 (MQ=255)
                                                                                                aCCCCGCGTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGCCCTTCGGGTTATGAGCCCGCCGAGCTACCAGGCTGCTCCACCCCGCGTCCCAGTCTGCCCACTATACAccccactcgatt                    >  5:674393/1‑141 (MQ=11)
                                                                                                 ctcttcgtatctgTACTGCTTTTATTTATTCAATTTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGtt                     <  8:276822/137‑1 (MQ=17)
                                                                                                 ccccGCGTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCGGGATGCGCACTATACTCCCCACTCGtttt                   >  3:1222472/1‑151 (MQ=11)
                                                                                                 ccccGCGTCACCGTACTGCTTTACTTCATAAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGtttt                   >  4:1439599/1‑151 (MQ=255)
                                                                                                  cccGCGTCACCGTACTGCTTTACTTCATCAAATTATAATTGGTTGCGGGGGCCGCATTTGAACCCACCACCTTCGGGTTATGAGCCCGACGAGCTACAAGGCTGCTCCACCCCGCGTCCGTGGATGCCCACTATACTCCGCTCGCGtttt                   >  6:937569/1‑150 (MQ=18)
                                                                                                   ccGCGTCACCGTACTGCTTTTACTTCATCAAATTTTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCAACCAGGCTGCTCCACCCCGCCTCACAGCAAGCCCACTATAcagcgatctattttta                  >  6:496492/1‑135 (MQ=11)
                                                                                                    cgcgTCACCGTACTGCTTTTACTTCATCAAATTTTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCCCGTCCGTGGATTGGCACAATACTccaatagagaattcat                >  3:375516/1‑137 (MQ=11)
                                                                                                    cgcgTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCAt                >  3:1125562/1‑151 (MQ=255)
                                                                                                    cgcgTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCAt                >  7:156744/1‑151 (MQ=255)
                                                                                                     gcgTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATg               >  2:226650/1‑151 (MQ=255)
                                                                                                      cgTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGAGGAATCGAACTATACACCGATAGAGTTTTCATGc              >  6:1116387/1‑151 (MQ=11)
                                                                                                      cgTCACCGTACTGCTTTTAATACATAAAATTTTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGc              <  5:1115961/151‑1 (MQ=17)
                                                                                                      cgTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGc              >  2:1017407/1‑151 (MQ=255)
                                                                                                      cgTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCAGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGc              >  5:753300/1‑151 (MQ=255)
                                                                                                       gTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGAAGCGCACTATACTCCGCTGGCGTTTTCGTggc             >  6:1265816/1‑149 (MQ=11)
                                                                                                       gTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATga              >  2:188508/1‑149 (MQ=255)
                                                                                                       gTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGc              >  6:747457/1‑150 (MQ=255)
                                                                                                       gTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCAGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGc              >  2:181785/1‑150 (MQ=255)
                                                                                                        tCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACAACGCTCGCGTTTTCAttga             >  6:613662/1‑147 (MQ=14)
                                                                                                        tCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCAATATACTACGCTCGGTTTttcattcac            >  6:123464/1‑150 (MQ=11)
                                                                                                        tCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCTGGATTCGCACTATACACCAAACGAGTATTCATGCa             >  6:1399320/1‑150 (MQ=1)
                                                                                                        tCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCa             >  8:1374371/1‑150 (MQ=255)
                                                                                                         cACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGAACTATACTCCGCTCGCTTTTTCATGCAAc           >  2:1046835/1‑151 (MQ=14)
                                                                                                         cACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAAc           >  3:1000854/1‑151 (MQ=255)
                                                                                                          aCCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAAcc          >  1:16895/1‑151 (MQ=255)
                                                                                                          aCCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAAcc          >  7:308757/1‑151 (MQ=255)
                                                                                                          aCCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAAcc          >  2:1453595/1‑151 (MQ=255)
                                                                                                           ccGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGAATCCCACAATACAACCAACGCTTTTTCATGCAACCt         >  5:1429601/1‑151 (MQ=11)
                                                                                                           ccGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGAGGATTCGCACTATACTCCGCTAGATTTTTCATTCAACCt         >  1:549364/1‑151 (MQ=11)
                                                                                                           ccGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGAGGATGCGCACTATACTCCGATCGCGATTTCATTCAACCt         >  7:382856/1‑151 (MQ=14)
                                                                                                            cGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGCGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCTGGAGGCCGTGTCTACTACGCTGGGGTTTTCAttgcgccgt        >  4:1274665/1‑143 (MQ=11)
                                                                                                            cGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGAGGCGGCTTATACTGCGCTCGCTTTTTCCTGCAAGCtt        >  3:1380246/1‑151 (MQ=11)
                                                                                                            cGTACTGCTTTTATTTCTTAAATTTTTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtt        <  8:383129/151‑1 (MQ=18)
                                                                                                            cGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtt        >  8:608788/1‑151 (MQ=255)
                                                                                                            cGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtt        >  1:662128/1‑151 (MQ=255)
                                                                                                             gTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCCCACTATACTCCGCACGCGttagatcggaagcgcc       >  7:276600/1‑137 (MQ=11)
                                                                                                             gTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGAGGATGCGAACTATAATCAAATGGAATTTTCATGCAACCtat       >  2:301181/1‑149 (MQ=11)
                                                                                                             gTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttt       >  5:1067502/1‑151 (MQ=255)
                                                                                                             gTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttt       >  3:1069509/1‑151 (MQ=255)
                                                                                                              tACTGCTTTTATTTCATAAATTTTTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttt       <  6:1431252/150‑1 (MQ=18)
                                                                                                              tACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTATAGTTTTCATGCACCCtttt      >  1:1280636/1‑151 (MQ=14)
                                                                                                              tACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGATGCGCACTATACTCCGCTGGAGTTTTCATGCACCCttgt      >  3:1055489/1‑149 (MQ=14)
                                                                                                              tACTGCTTTTACTTAATAAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGtt                     <  5:30272/136‑1 (MQ=14)
                                                                                                              tACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttt      >  2:1010807/1‑151 (MQ=255)
                                                                                                              tACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGCGTACGGATTTTAACCGACGACCTTCGGGTTATGAGCCCGACGAGCAACCAGGCTGCTCCACCCCGCGTCCGAGGATGCCCACAATACTCCGCTACCGTTTTCATGCAACCattt      >  6:1370357/1‑151 (MQ=18)
                                                                                                               aCTGCTTTTTCTTCATAAATTTTCATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttttt     <  4:377539/151‑1 (MQ=18)
                                                                                                               aCTGCTTTTACTTCATCAAATTTTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttttt     <  8:1122506/151‑1 (MQ=255)
                                                                                                               aCTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCAGTATACTCCTATCGCTTTTTCATGCAGCCttttt     >  7:1121986/1‑151 (MQ=14)
                                                                                                               aCTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGAGGCGCACTATACTCCGCTCGCGTTTTCATGCAAccgtgtt     >  3:94576/1‑146 (MQ=14)
                                                                                                                cTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttttt    >  4:193241/1‑151 (MQ=255)
                                                                                                                 tGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttttttt   >  4:114606/1‑151 (MQ=255)
                                                                                                                 tGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttttttt   >  8:308316/1‑151 (MQ=255)
                                                                                                                 tGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGACGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCAGTATACTCCGCTCGCGTTTTCATGCAACCttttttt   >  2:1294760/1‑151 (MQ=255)
                                                                                                                  gCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttttttt  >  8:584765/1‑151 (MQ=255)
                                                                                                                   cTTTACTTCATCAAATTTTATTTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttttttt  >  2:873216/1‑150 (MQ=255)
                                                                                                                   cTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttttttt  >  7:631907/1‑150 (MQ=255)
                                                                                                                   cTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttttttt  >  8:435101/1‑150 (MQ=255)
                                                                                                                   cTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttttttt  >  1:1050674/1‑150 (MQ=255)
                                                                                                                    |                                                                                                                                                     
TACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTT  >  DH1/936163‑936427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: