Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1936,2970AG41.5% 145.8 / 178.8 153intergenic (‑31/+3)ECDH1_RS04620/ECDH1_RS04625tRNA‑Met/tRNA‑Met
Reads supporting (aligned to +/- strand):  ref base A (70/13);  new base G (54/5);  total (127/26)
Fisher's exact test for biased strand distribution p-value = 3.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.23e-01

Only 100 of 157 total aligned reads displayed.

TACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGG‑ATGCGCACTATACTCCGCTCGCGTTTTCATGCAACC‑TTTTTTTTACCCCGATCACGGATTTGGC  >  DH1/936163‑936447
                                                                                                                                       |                                                                                                                                                        
tACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCAGGATATGACCCCACGACCTTCGGCTTATCAGCCATACCAGCTACCATGCAGCTCCACCCCGCGTAAAAGTACTGC‑TTTAGTTCGTCAAATTTTAATTGGTTGCggggg                                                                                                                                            >  1:452493/1‑149 (MQ=255)
                                         ggATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGTAGGCC‑GTGACTTCAGCGAAGTATATTTCGTTCCGCGGGGCGGTTTTGAACCGACGGCATTGGGGtttttagtgcgcccagc                                                                                                 >  5:405707/1‑136 (MQ=2)
                                                    gacgacCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGACGTATTTCAACAGACGACCTTAGGCTTATGAGACCGACTAGCTACCAGgctg                                                                                       >  8:1075308/1‑150 (MQ=14)
                                                                                 cTACCAGGCTGCTCCACCCCGCGTCACCGTAGTGG‑GTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGTTTTGAACCGCCGACCTTCGGGTTATGAGGCCGACGAGCTACCAGGCTGCTCCCCCCCGCGTCCGTGG‑ATGGGCACtat                                                        >  7:213334/1‑151 (MQ=17)
                                                                                         ctgctCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGG‑ATGCGCACTATACTCCGCt                                                >  8:605048/1‑151 (MQ=255)
                                                                                               cACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGG‑ATGCGCACTATACTCCGCTCGCGtt                                          >  6:273952/1‑151 (MQ=255)
                                                                                                aCCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGCCCTTCGGGTTATGAGCCCGCCGAGCTACCAGGCTGCTCCACCCCGCGTCCCAGT‑CTGCCCACTATACAccccactcgatt                                         >  5:674393/1‑141 (MQ=11)
                                                                                                 ccccGCGTCACCGTACTGC‑TTTACTTCATAAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGG‑ATGCGCACTATACTCCGCTCGCGtttt                                        >  4:1439599/1‑151 (MQ=255)
                                                                                                  cccGCGTCACCGTACTGC‑TTTACTTCATCAAATTATAATTGGTTGCGGGGGCCGCATTTGAACCCACCACCTTCGGGTTATGAGCCCGACGAGCTACAAGGCTGCTCCACCCCGCGTCCGTGG‑ATGCCCACTATACTCCGCTCGCGtttt                                        >  6:937569/1‑150 (MQ=18)
                                                                                                   ccGCGTCACCGTACTGCTTTTACTTCATCAAATTTT‑ATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCAACCAGGCTGCTCCACCCCGCCTCACAGC‑AAGCCCACTATAcagcgatctattttta                                       >  6:496492/1‑135 (MQ=11)
                                                                                                    cgcgTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGG‑ATGCGCACTATACTCCGCTCGCGTTTTCAt                                     >  3:1125562/1‑151 (MQ=255)
                                                                                                      cgTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGAGG‑AATCGAACTATACACCGATAGAGTTTTCATGc                                   >  6:1116387/1‑151 (MQ=11)
                                                                                                      cgTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGG‑ATGCGCACTATACTCCGCTCGCGTTTTCATGc                                   >  2:1017407/1‑151 (MQ=255)
                                                                                                      cgTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCAGTGG‑ATGCGCACTATACTCCGCTCGCGTTTTCATGc                                   >  5:753300/1‑151 (MQ=255)
                                                                                                       gTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGG‑AAGCGCACTATACTCCGCTGGCGTTTTCGTggc                                  >  6:1265816/1‑149 (MQ=11)
                                                                                                       gTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGG‑ATGCGCACTATACTCCGCTCGCGTTTTCATga                                   >  2:188508/1‑149 (MQ=255)
                                                                                                       gTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGG‑ATGCGCACTATACTCCGCTCGCGTTTTCATGc                                   >  6:747457/1‑150 (MQ=255)
                                                                                                       gTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCAGCGTCCGTGG‑ATGCGCACTATACTCCGCTCGCGTTTTCATGc                                   >  2:181785/1‑150 (MQ=255)
                                                                                                        tCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGG‑ATGCGCAATATACTACGCTCGGTTTttcattcac                                 >  6:123464/1‑150 (MQ=11)
                                                                                                        tCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCTGG‑ATTCGCACTATACACCAAACGAGTATTCATGCa                                  >  6:1399320/1‑150 (MQ=1)
                                                                                                        tCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGG‑ATGCGCACTATACTCCGCTCGCGTTTTCATGCa                                  >  8:1374371/1‑150 (MQ=255)
                                                                                                         cACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGG‑ATGCGAACTATACTCCGCTCGCTTTTTCATGCAAc                                >  2:1046835/1‑151 (MQ=14)
                                                                                                         cACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGG‑ATGCGCACTATACTCCGCTCGCGTTTTCATGCAAc                                >  3:1000854/1‑151 (MQ=255)
                                                                                                          aCCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGG‑ATGCGCACTATACTCCGCTCGCGTTTTCATGCAAcc                               >  1:16895/1‑151 (MQ=255)
                                                                                                           ccGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGAGG‑ATGCGCACTATACTCCGATCGCGATTTCATTCAACC‑t                             >  7:382856/1‑151 (MQ=14)
                                                                                                            cGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGCGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCTGG‑AGGCCGTGTCTACTACGCTGGGGTTTTCAttgcgccgt                             >  4:1274665/1‑143 (MQ=11)
                                                                                                            cGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGG‑AGGCGGCTTATACTGCGCTCGCTTTTTCCTGCAAGC‑tt                            >  3:1380246/1‑151 (MQ=11)
                                                                                                            cGTACTGC‑TTTTATTTCTTAAATTTTTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGG‑ATGCGCACTATACTCCGCTCGCGTTTTCATGCAACC‑tt                            <  8:383129/151‑1 (MQ=18)
                                                                                                            cGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGG‑ATGCGCACTATACTCCGCTCGCGTTTTCATGCAACC‑tt                            >  8:608788/1‑151 (MQ=255)
                                                                                                             gTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGAGG‑ATGCGAACTATAATCAAATGGAATTTTCATGCAACC‑tat                           >  2:301181/1‑149 (MQ=11)
                                                                                                             gTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGG‑ATGCGCACTATACTCCGCTCGCGTTTTCATGCAACC‑ttt                           >  5:1067502/1‑151 (MQ=255)
                                                                                                              tACTGCTTTTATTTCAT‑AAATTTTTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGG‑ATGCGCACTATACTCCGCTCGCGTTTTCATGCAACC‑ttt                           <  6:1431252/150‑1 (MQ=18)
                                                                                                              tACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGG‑ATGCGCACTATACTCCGCTATAGTTTTCATGCACCC‑tttt                          >  1:1280636/1‑151 (MQ=14)
                                                                                                              tACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGG‑ATGCGCACTATACTCCGCTGGAGTTTTCATGCACCC‑ttgt                          >  3:1055489/1‑149 (MQ=14)
                                                                                                              tACTGCTTTTACTTAAT‑AAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGG‑ATGCGCACTATACTCCGCTCGCGtt                                          <  5:30272/136‑1 (MQ=14)
                                                                                                              tACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGCGTACGGATTTTAACCGACGACCTTCGGGTTATGAGCCCGACGAGCAACCAGGCTGCTCCACCCCGCGTCCGAGG‑ATGCCCACAATACTCCGCTACCGTTTTCATGCAACC‑attt                          >  6:1370357/1‑151 (MQ=18)
                                                                                                               aCTGCTTTTTCTTCAT‑AAATTTTCATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGG‑ATGCGCACTATACTCCGCTCGCGTTTTCATGCAACC‑ttttt                         <  4:377539/151‑1 (MQ=18)
                                                                                                               aCTGCTTTTACTTCATCAAATTTT‑ATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGG‑ATGCGCACTATACTCCGCTCGCGTTTTCATGCAACC‑ttttt                         <  8:1122506/151‑1 (MQ=255)
                                                                                                               aCTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGG‑ATGCGCAGTATACTCCTATCGCTTTTTCATGCAGCC‑ttttt                         >  7:1121986/1‑151 (MQ=14)
                                                                                                               aCTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGG‑AGGCGCACTATACTCCGCTCGCGTTTTCATGCAAccgtgtt                          >  3:94576/1‑146 (MQ=14)
                                                                                                                cTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGG‑ATGCGCACTATACTCCGCTCGCGTTTTCATGCAACC‑tttttt                        >  4:193241/1‑151 (MQ=255)
                                                                                                                 tGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGACGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGG‑ATGCGCAGTATACTCCGCTCGCGTTTTCATGCAACC‑ttttttt                       >  2:1294760/1‑151 (MQ=255)
                                                                                                                  gtttttACTTC‑TTAAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGG‑ATGCGCACTATACTCCGCTCGCGTTTTCATGCAACC‑tttttttt                      <  7:258561/148‑1 (MQ=18)
                                                                                                                  gcttttACTTC‑TAAAAATTTCATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGG‑ATGCGCACTATACTCCGCTCGCGTTTTCATGCAACC‑ttttttt                       <  7:449678/147‑1 (MQ=18)
                                                                                                                   tcctttctttcataaaaTTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGG‑ATGCGCACTATACTCCGCTCGCGTTTTCATGCAACC‑tttttt                        <  3:323955/136‑1 (MQ=18)
                                                                                                                   cttttACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGG‑ATTCGCAGTATACTACGCAGGCGTTTTCATGCAACC‑tttttttt                      >  6:388815/3‑150 (MQ=17)
                                                                                                                   cttttACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGG‑ATGCGCACTATACTCCGCTCGCGTTTTCATGCAACC‑tttttttt                      >  4:109183/3‑150 (MQ=255)
                                                                                                                   cttttACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCAGG‑ATTCGCACAATACAACAATATATATTTCATGCAACC‑tttttttt                      >  8:279334/3‑150 (MQ=1)
                                                                                                                   c‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGG‑ATGCGCACTATACTCCGCTCGCGTTTTCATGCAACC‑tttttttt                      >  1:1050674/1‑150 (MQ=255)
                                                                                                                   c‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGG‑ATGCGCACTATACTCCGCTCGCGTTTTCATGCAACC‑tttttttt                      >  8:435101/1‑150 (MQ=255)
                                                                                                                   c‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGG‑ATGCGCACTATACTCCGCTCGCGTTTTCATGCAACC‑tttttttt                      >  7:631907/1‑150 (MQ=255)
                                                                                                                    ttttACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCAGT‑AAGCCCAATATACTACACTCTATAATTCATTCAACC‑TTAATTTTat                    >  8:258830/2‑150 (MQ=11)
                                                                                                                     tttACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGG‑ATGCGCAGTATACTCCGCTCTGTTTTTCCTGCCCCC‑GTTTTTTTaac                   >  1:1179076/1‑149 (MQ=14)
                                                                                                                     tttACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCAGGGG‑ATGCCCATTATACTACGATCTCGATTTCGTGGAAGC‑gttctttaaac                   >  1:74733/1‑147 (MQ=1)
                                                                                                                      ttACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGG‑ATGCGCACTATACTCCGCTCGCGTTTTCATGCAACC‑TTTTTTTTAcc                   >  1:1034243/1‑151 (MQ=255)
                                                                                                                      ttACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGG‑ATGGGGACTATACTCCGATATAGTTTTCATGCACCC‑GTTTTTTTAgcc                  >  3:93984/1‑151 (MQ=14)
                                                                                                                      ttACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGG‑ATGCGCACTATACTCCGATCGGGTTTTCATGCAACC‑TTGTTGTTAacc                  >  6:334682/1‑151 (MQ=17)
                                                                                                                      ttACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGG‑ATGCGCACTATACTACGCTAGGGTTTTCATTCAGCC‑TTGTTTTTAacc                  >  5:1049977/1‑151 (MQ=14)
                                                                                                                      ttACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCCGGAATGCG‑ACAATACTGCAATCTCTTATTCCTGCAACC‑GTTCTTTTagc                   >  3:711050/1‑148 (MQ=11)
                                                                                                                      ttACTTAAT‑AAATTTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGG‑ATGCGCACTATACTCCGCTCGCGttt                                         <  7:464528/129‑1 (MQ=255)
                                                                                                                       tACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGT‑ATTCGCACTATACTCCGCAATAGTTTTCATGCAACC‑TTTTTTTTaacac                 >  4:341005/1‑147 (MQ=17)
                                                                                                                       tACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGG‑AATCGAACTATACAACCCTATAGATTTCATGCAACC‑TTTTTTTTaacac                 >  6:1320151/1‑147 (MQ=11)
                                                                                                                       cgttagggtgttcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGG‑ATTCGCACTATACTCCGATAGCTTTTTCATTCAACC‑TTTTTTTTAcc                   >  4:1423837/16‑150 (MQ=14)
                                                                                                                       cgttagggtgttcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCAGCGTCCGTGG‑ATGCGCACTATACTCCGCTCGAGTTTTCATGCAACC‑TTTTTTTTAccc                  >  8:633032/16‑151 (MQ=18)
                                                                                                                         ttagggtgttcattAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGG‑ATGCGCACTATACTCCGCTCGCGTTTTCATGCAACC‑TTTTTTTTAcccc                 <  4:273942/138‑1 (MQ=255)
                                                                                                                         ttagggtgttcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGT‑ATGCGCACTATACTCCGCTCTCTTTTTCATGCAACC‑tttttttt                      >  2:281649/14‑145 (MQ=17)
                                                                                                                         ttagggtgttcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGG‑ATGCGCACTATACTCCGCTCGCGTTTTCCTGCACGC‑GTTTTTTTACCCCg                >  1:903145/14‑151 (MQ=17)
                                                                                                                         ttagggtgttcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGG‑ATGCGCACTATACTCCGCTCGCGTTTTCATGCAACC‑tttttttt                      <  1:280876/132‑1 (MQ=255)
                                                                                                                         cTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGG‑ATGCGCACTATACTCCGCTCGGGTTTTCGTGCAACC‑TTGTTTTTTCCCCgc               >  1:179220/1‑150 (MQ=17)
                                                                                                                         cTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCAGTGG‑AGGCGCACTATACTCCACTCGCTATTTCATTGAGCC‑GTTTTTTTAACCCg                >  1:654294/1‑150 (MQ=14)
                                                                                                                          ttCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGAGT‑AATCGCACTATACTCCGCTATAGTATTCATTCCACC‑TTGTTTTTAACCCCAt              >  8:1117379/1‑151 (MQ=11)
                                                                                                                          ttCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCAACCAGCGTCCCAGG‑ATGCGCACTATACTCCAATCGATATTTAATTCAACC‑TTAATTATAccaacat              >  2:1273504/1‑146 (MQ=11)
                                                                                                                          tagggtgttcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGG‑ATGCGCACTATACTCCGCTCGCTTATTCATGCACCC‑ttttt                         >  7:281228/13‑141 (MQ=17)
                                                                                                                           tcatcaAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGG‑ATGCGCACTATACTCCGCTCGCGTTTTCATGCAACC‑TTTTTTTTACCCCGAt              >  8:471916/1‑151 (MQ=255)
                                                                                                                            ggttgttcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGG‑ATGCGCACTATACTCCGCTCGCGTTTTCATGCAACC‑TTTTTTTTACCCCGATc             <  5:868344/141‑1 (MQ=255)
                                                                                                                            catcaAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGG‑ATGCGCACTATACTCCGCTCGCGTTTTCATGCAACC‑TTTTTTTTACCCCGATc             >  3:441288/1‑151 (MQ=255)
                                                                                                                             ggtgttcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGG‑ATGCGCACTATACTCCGCTCGCGTTTTCATGCAACC‑TTTTTTTTACCCCGATCa            <  3:352437/142‑1 (MQ=255)
                                                                                                                             atcaAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGG‑ATGCGCACTATACTCCGCTGGAGTTTTCATGCAACC‑TTTTTTTTACCCCGATCAc           >  8:1227534/1‑151 (MQ=255)
                                                                                                                             atcaAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGG‑ATGCGCACTATACTACGCTAGAGTTTTCATGCAACC‑TTTTTTTTACCCCGATCAc           >  4:163388/1‑151 (MQ=18)
                                                                                                                              gtgttcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGG‑ATGGGCACTATACTCCGCTCGGGTTTTCATGCACCCTTTTTTTTTACCCCGATc             >  3:1424358/9‑150 (MQ=18)
                                                                                                                              gtgttcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGG‑ATGCGCACTATACTCCGCTCGCGTTTTCATGCAACC‑TTTTTTTTACCCCGATCAc           <  3:1173968/143‑1 (MQ=255)
                                                                                                                               tgttcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGG‑ATGCGCACTATACTCCGATCGATTTTTCATGCAACC‑TTTATTTTACCCCGATCAcc          >  8:1208451/8‑150 (MQ=18)
                                                                                                                                gttcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGG‑ATGCGCACTATACTCCg                                                  <  7:65303/105‑1 (MQ=255)
                                                                                                                                aaaTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGG‑ATGCGCACTATACTCCGCTCGCGTTTTCATGCAACC‑TTTTTTTTACCCCGATCACgg         >  7:1315646/1‑151 (MQ=255)
                                                                                                                                 aaTTTTTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCTGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGG‑ATGCGCACTATACTCCGCTCGCGTTTTCATGCAACC‑TTTTTTTTACCCCGATCACGGa        <  2:550609/151‑1 (MQ=255)
                                                                                                                                 aaTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCAGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGG‑ATGCGCACTATACTCCGCTCGCGTTTTCATGCAACC‑TTTTTTTTACCCCGATCACgg         >  4:798634/1‑150 (MQ=255)
                                                                                                                                  tcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGG‑ATGCGCACTATACTCCGCTCGCGTTTTCATGCACCC‑ttttttt                       >  3:231301/5‑135 (MQ=255)
                                                                                                                                  aatttGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGG‑ATGCGGAGTATACTGCGCTCGCGTTTTCCTGCACGG‑GTTTTTTTAACCCGCTCCGGGAt       >  7:39125/3‑151 (MQ=14)
                                                                                                                                   cattAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGG‑ATGCGCACTATACTCCGCTCGCGTTTTCATg                                    <  5:1108168/120‑1 (MQ=255)
                                                                                                                                   caatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGG‑ATGCGCACTATAc                                                      >  5:183054/4‑104 (MQ=255)
                                                                                                                                   caatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGG‑ATGCGCACTATACTCCGCTCGCGTTTTCATGCAACC‑TTTTTTTTACCCCGATCACGGAt       <  8:495513/147‑1 (MQ=255)
                                                                                                                                   atttGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGAGG‑ATGCGCAGTATACTACGCTCGATTTTTCATGCAACC‑TTTATTATAACCCCATCACGGAtt      >  2:1174085/2‑151 (MQ=17)
                                                                                                                                    ttttATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGG‑ATGCGCACTATACTCCGCTCGCGTTTTCATGCAACC‑TTTTTTTTACCCCGATCACGGAtt      <  4:1226385/150‑1 (MQ=255)
                                                                                                                                    tttGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGG‑ATGCGCACTATACTCCGCTCGATTTTTCATGCAACC‑TTTTTTTTACCCCGATCACGGAttt     >  2:570669/1‑151 (MQ=255)
                                                                                                                                    aatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGT‑ATGCCAACTATACTCCGCTATATTTTTCATGCACCC‑TTATTTTTAACCCGATCACGCAttt     >  2:803883/3‑151 (MQ=17)
                                                                                                                                     atAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCg                                                                       >  6:1332393/2‑86 (MQ=14)
                                                                                                                                     atAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCg                                                                       <  5:1331587/85‑1 (MQ=14)
                                                                                                                                      tAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGG‑ATGCGCACTATACTCCGCTCGCGTTTTCATGCAACC‑TTTTTTTTACCCCGATCACGGATTTgg   >  6:845321/1‑151 (MQ=255)
                                                                                                                                       aaTTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGG‑ATGCGCACTATACTCCGCTCGCGTTTTCATGCAACC‑TTTTTTTTACCCCGATCACGGATTTGGc  >  6:1119505/1‑151 (MQ=255)
                                                                                                                                       aaTTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGG‑ATGCGCACGATACTCAGCTCGCGGTTTCATGCAACC‑TTTTTTTTACCCCGATCACGGATTTGGc  >  5:1479194/1‑151 (MQ=255)
                                                                                                                                       |                                                                                                                                                        
TACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGG‑ATGCGCACTATACTCCGCTCGCGTTTTCATGCAACC‑TTTTTTTTACCCCGATCACGGATTTGGC  >  DH1/936163‑936447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑