| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | DH1 | 1,065,605 | 0 | A | T | 28.5% | 230.0 / 93.7 | 158 | intergenic (+40/‑23) | ECDH1_RS05245/ECDH1_RS05250 | tRNA‑Arg/tRNA‑Arg |
| Reads supporting (aligned to +/- strand): ref base A (61/52); new base T (32/13); total (93/65) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.14e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01 | |||||||||||
Only 100 of 163 total aligned reads displayed.
AAAAAATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAG‑CGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTA > DH1/1065458‑1065754 | aaaaaaTCTTCAGTAAAGTAAGTTTCCCGATGCATACGTAGCTCAGCTGGATAGACTAATCGGCTACGAACCGAT‑CGGTCTGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATcagca < 8:325482/151‑1 (MQ=21) tatgtaatgtaaGATTCCCTATGCATCCGTAGCTGATCTGTATAGAGTACTCGGCTACGAAGCGAT‑CGGTCTGAGGTTCTAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAaaata < 8:64100/147‑1 (MQ=21) gCAGAAAAGTAATTTGCCCTCTGCATCCGGAGCTCACCGGGAGAGAGGAATCGGCTACTAACGGTA‑GGCGCCGAGGTTCGAATCCTCCCGGATGCACCATCTCTTAATTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAgaataa < 3:953812/151‑1 (MQ=9) aaagtaaGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAG‑CGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTc < 7:177116/151‑1 (MQ=255) agtaagtTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAG‑CGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTCATAGATATAGCGTAATGAACGGAAAC‑‑AAT‑‑ATGAACAGTAATTTAA‑ATCTccc > 8:1157929/1‑151 (MQ=14) tttcctttcttttGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAGCGAT‑CGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTcc < 6:192850/136‑1 (MQ=21) aacagccctttGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAG‑CGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATg < 1:1365961/141‑1 (MQ=255) gccatttGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAG‑CGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATg < 8:1474586/141‑1 (MQ=255) cccGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAG‑CGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTAGATATGGCTATAGTAGCGGTATC‑‑AAT‑‑ATAAACAGTAAAAAAA‑AAGTCCCTATGCaaac > 4:486493/1‑148 (MQ=18) tttGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAG‑CGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATGATACTTGATATGCCTTTAGTAGCGGTATA‑‑AAT‑‑ATAAGCAGTAAAAAAA‑ATATCCAGaacggaag > 5:193151/3‑141 (MQ=17) cGTATCTCAGCTGGATATACTACTCGGCGACGATCGCTC‑CGCTCGGAGCGTCTAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCt < 7:566697/151‑1 (MQ=17) aGCTCATCTTGATTGAGTAATCGGCTACTAAGGGTT‑GGGTCTGAGGTTGGAATCCTCCCGGATGCACCATCTCTTAATTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAgaataagt < 4:88402/123‑3 (MQ=11) aGCTGGATAGAGTACTCGGCTACGAACCGAG‑CGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATATTACTTCATATTGCTATAGTAGCGGTATC‑‑AAT‑‑ATCTGCAGTAAATTAA‑ATAACCCTATGCATCGGTTGcacatcgggttaca > 4:1461558/1‑140 (MQ=17) gtttttctaatCGGCGACGATCCCAC‑CGCGCGGCGCTTCTAATCCTCCGGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACt < 7:1110951/140‑1 (MQ=14) agagTACTCGGCTACGAACCGAT‑CGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTAATTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAgaata < 7:431072/107‑1 (MQ=255) aCTCGGCTACGAACCGTT‑GGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTAATTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAGAATAAGTTTTCCCGATGCATCCGTa < 5:402910/123‑1 (MQ=11) cTCGGCTACGAACCGAG‑CGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATAGTACTAGATCTGGCTGAAGTATCGGTATC‑‑AAT‑‑ATCTGCAGGAATTAAA‑ATTTCGCCATTCCTCGGAACCGCAGCATGATAGACTACTCCGCTACGac > 5:1481501/1‑150 (MQ=9) tCGGCTACGAACCGAG‑CGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAGAATAA‑GTTTtccccttgca > 1:826596/1‑108 (MQ=17) cactaCCGAGCCGCTCGGAGCTTCTAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGc < 7:1278808/147‑1 (MQ=14) gatcgctccGGTCGGCGCTTCTAATCCTCCCTTATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGt < 3:977382/143‑1 (MQ=14) gctccgctCGGAGAGTCTAATCCTCCCGGATTCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGa < 7:854225/144‑1 (MQ=14) gtatgttctgAGGTTCTTATCCTCCGGTATTCAAGATCTTTTTTTTGATATGTCTTTTTTAGGGTTATT‑‑AAT‑‑ATCAGCAGTAAAATAA‑TTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAg < 4:1318842/142‑1 (MQ=2) ggctcttagagtctaaTCCTCGCGTATTCAACATCTCTTACTTTATATGTCTTTACTAGCTCTATA‑‑AAT‑‑ATCAGCAGTAAAATAA‑CTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGtt < 3:825151/137‑1 (MQ=11) cGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGGAGCGGTATG‑‑AAG‑‑AGCAGGAGGAAAATTA‑CTTTCCCTATGCAGCCGTAGCACAGCTGGATAGAGTACTCGGCTGGGCCCGGCGCGGTGGGAGGtt > 7:371251/1‑151 (MQ=17) ggTCGGAGGTTCGAATCCTCCCGGATGCACCATATCGTACTTCATATGGCTGTAGTAGCGGTAGC‑‑AAG‑‑ATCTGGAGGAAAATAA‑ATTACCCGATGCGTAGGTAGGACAGATCGATAGAGTAATCGGATACGAACCGAGCGGTCGGAGGTTc > 1:173108/1‑151 (MQ=17) gctctgcgcgtctaaTCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTc < 7:181930/138‑1 (MQ=255) ggcgagtcttaTCCGCCGGGAGTCACCATCTCTTACTTGATATGGGTTTAGTAGCGCTATA‑‑AAT‑‑ATCAGCAGTAAACTAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAAt < 1:1027021/141‑1 (MQ=14) ggAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑ATC‑‑TGAAGCAGTAAAGTAT‑ATTTCCCGATCCAGCCGTTCCGGATCTGCTTAGAGATCACGGCTACGAACACAGCGGGCGGAGGttggccg > 7:325540/1‑146 (MQ=9) ggcgtctaaTCCTCCCGGATGCACCATCTCTTACTTGATATGTCTTTACTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAAtcc < 1:256040/144‑1 (MQ=255) ttCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAgaat > 1:995274/1‑73 (MQ=25) tCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCGTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCGTCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCCAGCGGTCGGAGGTTCGAATCCTccc > 3:541998/1‑151 (MQ=255) gtaaTCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCg < 1:355662/149‑1 (MQ=38) gAATCCTCCCGGATGCACCATATAGTACTTGATATTCATGTATTAGCGTTAAC‑‑AAT‑‑ATCAACAGTAAAAAAA‑ATTTCCCGATACATACGAAGAGCATCTCGATAGATTAATCGGCTACGAAACTAGATGTCGGAGGTTCGAATCCTCCCgg > 4:987119/1‑151 (MQ=9) gAATCCACCCGGATGCACCATCTCTTACTTGAAAGCGCTATACTAGCGGTATA‑‑AAT‑‑ATCAGCAGTAAATTAA‑CTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGATCGGTCGGAGGTTCGAATCCTCCCgg < 7:1007488/151‑1 (MQ=14) aatcctcggcgtcccACTATCTCTTTTTTTATATGTTTTTATTAGCTCTATA‑‑AAT‑‑ATCAGCAGTAAACTAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTccc < 2:1155592/134‑1 (MQ=11) aagcctccCGGATCCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATA‑‑AAT‑‑ATCAGCAGTAAACTAA‑CTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCg < 7:1000131/146‑1 (MQ=255) aaTCCTCCCTGATGCAAGATCTCTTACTTTATATCGCTTTAGGAGCTGTATC‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCgg < 7:1445061/150‑1 (MQ=14) aTCCTCCGGTATTCACGATCTCTTACTTGATATGGCTTTAGTAGCTCTCTA‑‑AAT‑‑ATCAGCAGTAAAATAA‑CTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTTGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGAt < 3:353448/151‑1 (MQ=11) aTCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTag > 2:675284/1‑64 (MQ=17) ccctccCGGATGGACTATCTTTTTCTTGATATGTATTTAGTAGCTGTATC‑‑AAT‑‑ATCTGCAGTAAAATAA‑TTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATg < 3:1051690/150‑1 (MQ=11) catccCAGATCCACGATCTCTTACTTGATATGGCTATACCAGCGGTATA‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATg < 3:371277/148‑1 (MQ=14) ctccCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTAAC‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCAGGATGc > 1:47439/1‑150 (MQ=255) ttccgttgaagcATCTCTTACTTTATTTGTTTTTTTTAGGTTTAAA‑‑AAT‑‑ATCTGCAGTAAATTAA‑TTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATg < 4:36990/136‑1 (MQ=1) cccGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGt > 1:972430/1‑58 (MQ=35) cGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAGCCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCat > 7:116548/1‑151 (MQ=255) gATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTACCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCatat > 5:598371/1‑151 (MQ=255) aTGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTag > 7:1156091/1‑54 (MQ=18) aTGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTag > 7:612806/1‑54 (MQ=18) aTGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTag < 8:1156666/55‑2 (MQ=18) cACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTACCCATTCATACGTACCTGATCTTGATAGAGTAATACGCTACGAACCCACCCGTGCGATGTTAGAATCCTGCCCGaatcaacaaaatat > 8:310017/1‑137 (MQ=2) aCCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATtatc > 2:584460/1‑150 (MQ=255) cATCTCTTACTTGATCTGGCTATACGAGCTCTATA‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGa < 7:1250538/151‑1 (MQ=14) atatttttcTTTATATTGTTTTAGTAGAGGTATC‑‑TAT‑‑TTCTTTATTAAAGTAA‑TTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGaa < 8:427791/143‑1 (MQ=11) tctcTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGaa > 8:29018/1‑150 (MQ=255) tctcTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGaa > 2:871921/1‑150 (MQ=255) tctcTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGaa > 2:952803/1‑150 (MQ=255) ctTACTTTATATCTCTTTAGTAGCTCTATA‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGaaa < 1:359317/148‑1 (MQ=14) tACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTc > 5:9476/1‑151 (MQ=255) agttgatcgctcTTTACTAGGGTTAAC‑‑AAT‑‑ATCAGCAGTAAACTAA‑CTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCa < 3:1383305/140‑1 (MQ=14) aCTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATGCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACGGAGCGGACCCCGGTTCAAATCATTCCCGAAGATCCATATtctaagccctcttct > 1:851580/1‑139 (MQ=14) cTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTc > 1:81555/1‑149 (MQ=255) ttGATACGGCTTTAGTAGCGGTATCAAAAA‑‑ATCTGCGGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCTAGATCTTcc > 1:1096613/1‑150 (MQ=14) ttGATACGGCTTTAGTAGCGGTATCAAAAA‑‑ATCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATAttatagctgaagagcg > 2:1230702/1‑137 (MQ=14) ttGATACGGCTTTAGTAGCGGTATCAAAAA‑‑ATCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTACTAAATATTc > 8:595526/1‑150 (MQ=14) ttGATACGGCTTTAGTAGCGGTATCAAAAA‑‑ATCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATAGTACGAAAtatgc > 2:1413352/1‑146 (MQ=14) tGATACGGCTTTAGTAGCGGTATCAAAAA‑‑ATCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATACTACTAAATATTc > 7:720576/1‑149 (MQ=14) gATACGGCTTTAGTAGCGGTATCAAAAA‑‑ATCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTATCCGAAATATTCatt > 6:2542/1‑149 (MQ=14) gATACGGCTTTAGTAGCGGTATCAAAAA‑‑ATCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTATACGAAATATTCAgt > 8:639253/1‑150 (MQ=14) atacGGCTTTAGTAGCGGTATCAAAAA‑‑ATCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCTAAAtatctcgg > 5:449771/1‑143 (MQ=14) aatgggtattctagCGCTAAA‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATg < 5:705160/140‑1 (MQ=14) acggCTTTAGTAGCGGTATCAAAAA‑‑ATCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTATCCTAAATAttgatctat > 1:710479/3‑144 (MQ=14) ggCTTTAGTAGCGGTATCAAAAA‑‑ATCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTGTCCTACATATTCAGCAAtt > 5:656339/1‑149 (MQ=14) ggCTTTAGTAGCGGTATCAAAAA‑‑ATCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCTATATATTCataaatt > 3:125374/1‑144 (MQ=14) ggCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGTAAACTTCAGCAATGa > 5:1174962/1‑149 (MQ=25) tttAGTAGCGGTATCAAAAA‑‑ATCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTACGAAATATTCAGCAATTAAgg > 6:87469/1‑151 (MQ=11) tttAGTAGCGGTATCAAAAA‑‑ATCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATAGTCCGAAATATTCCGAAATGAAgg > 8:1231412/1‑151 (MQ=11) tttAGTAGCGGTATCAAAAA‑‑ATCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCAAATCCTCCCGGATGCACCATATTATACTAAATATTCATAAATTAAgg > 4:975218/1‑151 (MQ=14) tttAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGTAAACTTCAGCAATGAAGGtt > 5:1335511/1‑150 (MQ=25) ttAGTAGCTTTATA‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTa < 7:614797/150‑1 (MQ=255) agtagCGGTATCAAAAA‑‑ATCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTATACTAAATATTGAGCAATGAACGTAt > 5:28755/1‑151 (MQ=11) ttgCGGTATC‑‑AAAAAATCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGc < 6:16225/136‑1 (MQ=11) tagCGGTATCAAAAA‑‑ATCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTATACGAAATCTTCATAAATGAACGTAtac > 8:254074/1‑149 (MQ=11) gCGGTATC‑‑AAAAAATCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTATCCTTAATCTGCATAAATGAAGGTaagga > 8:408371/1‑146 (MQ=11) cGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAAtc < 2:850018/98‑1 (MQ=255) cGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAag > 5:639580/1‑149 (MQ=255) cGGTATC‑‑AAT‑‑ATCAGAAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGt < 5:1318075/84‑1 (MQ=255) ggTATC‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTc > 3:1412165/1‑92 (MQ=255) ggTATC‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAgg > 2:590355/1‑151 (MQ=255) ggTATC‑‑AAT‑‑ATCAGCAGT‑AAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATGGAAGAGGt > 8:787962/1‑151 (MQ=255) ttata‑‑AAT‑‑ATCTGCAGTAAATTAA‑TTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAgg < 2:1470469/149‑1 (MQ=255) atcaaaaaatCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGACATATTCAGCTATTAAGGTAATGAAGAGGt > 3:860734/9‑151 (MQ=11) atcaaaaaatCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGAGGTCTTAGGTTCGAATCCTCAAGGATTCACCATATTATACGAAATATTCAGAAATGAACGTATAGaatatc > 6:1168596/9‑146 (MQ=11) aaT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGACCCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATATTCAGCAATGAAGGAATCGAAGAGGTAGCgg > 4:1052218/1‑151 (MQ=255) t‑‑atCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTc > 6:1308789/1‑57 (MQ=255) t‑‑atCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGa > 2:230551/1‑150 (MQ=255) tCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCt < 1:528452/59‑1 (MQ=255) tCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAg < 5:167347/78‑1 (MQ=255) tCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAAtt > 2:1056115/1‑151 (MQ=255) cagcagTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCCGTCGGAGGTTCGAATAGTCGCGGATGCACAATATTCTCCGAAATCTGCAGGATGGAGGGTATCGATGAGGTTGCGGAtttg > 3:225165/1‑150 (MQ=14) cagcagTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGATCTCGGCTGCGAACCGCGCCCGCGGAGGTTCGAATACTCCCGGATGCACCATAGTGTCCGAAATCTACAGCAATGAAGGTTGCGAAGGGGGAGCggcgtgc > 7:69185/1‑146 (MQ=14) | AAAAAATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAG‑CGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTA > DH1/1065458‑1065754 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |