Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH11,165,4440AC22.7% 646.0 / 48.2 279T71P (ACG→CCG) yfiFmethyltransferase
Reads supporting (aligned to +/- strand):  ref base A (43/172);  new base C (63/0);  total (106/173)
Fisher's exact test for biased strand distribution p-value = 4.10e-34
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 280 total aligned reads displayed.

ATGATTCTGATAAACGTACCCACAACCCGCGTACCGGGAAAGGGGGCGGGCGTCCAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑CACGGTTTCCCGCGCGCCGGGTGATGAGACGCCGGAAAAGGCCGATCACGGTGGCATCAGTGG‑TAAAAGTTTTATTGATCCGGAAGTGTTGCGTCGTCAGCGTGCGGAAGAAACCCGCGTCTACGGCGAAAATGCATGTCAGGCACTTTTCC  >  DH1/1165295‑1165594
                                                                                                                                                       |                                                                                                                                                       
 tgatTCTGATAAACGTACCCACAACCCGCGTACCGGGAAAGGGGGCGGGCGTCCAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGGG‑CAcc                                                                                                                                                       >  1:650240/1‑150 (MQ=255)
   atTCTGATAAACGTACCCACAACCCGCGTACCGGGAAAGGGGGCGGGCGTCCAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑CACGGt                                                                                                                                                     <  3:62990/151‑1 (MQ=255)
    ttCTGATAAACGTACCCACAACCCGCGTACCGGGAAAGGGGGCGGGCGTCCAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGGCG‑CCCGGt                                                                                                                                                     >  4:898859/1‑151 (MQ=255)
     tCTGATAAACGTACCCACAACCCGCGTACCGGGAAAGGGGGCGGGCGTCCAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑CACGGtt                                                                                                                                                    <  6:454987/150‑1 (MQ=255)
        gATAAACGTACCCACAACCCGCGTACCGGGAAAGGGGGCGGGCGTCCAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑CACGGttt                                                                                                                                                   <  7:1353699/148‑1 (MQ=255)
          tAAACGTACCCACAACCCGCGTACCGGGAAAGGGGGCGGGCGTCCAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGGG‑CCCCGGTTGCcgc                                                                                                                                              >  4:1414889/1‑151 (MQ=255)
                   ccACAACCCGCGTACCGGGAAAGGGGGCGGGCGTCCAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑CACGGTTTCCCGCGCGCCGGGt                                                                                                                                     <  8:886960/151‑1 (MQ=255)
                    cacaACCCGCGTACCGGGAAAGGGGGCGGGCGTCCAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑CACGGTTTCCCGCGCGCCGGGtg                                                                                                                                    <  3:69301/151‑1 (MQ=255)
                     acaACCCGCGTACCGGGAAAGGGGGCGGGCGTCCAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑CACGGTTTAACGCGCGCCGCGtgc                                                                                                                                   >  6:611770/1‑150 (MQ=255)
                       aaCCCGCGTACCGGGAAAGGGGGCGGGCGTCCAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑CACGGTTTCCCGCGCGCCGGGtgatg                                                                                                                                 <  3:128017/151‑1 (MQ=255)
                       aaCCCGCGTACCGGGAAAGGGGGCGGGCCTCCAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCC‑GACGCGTTAGCGCGGGCCgggggggg                                                                                                                                 >  2:505846/1‑146 (MQ=255)
                        aCCCGCGTACCGGGAAAGGGGGCGGGCGTCCAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑CCCGGTTTCCCGCGCGCCCGGtgatg                                                                                                                                 >  4:346559/1‑150 (MQ=255)
                         cccGCGTACCGGGAAAGGGGGCGGGCGTCCAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑CACGGTTTCCCGCGCGCCGGGTGATgag                                                                                                                               <  1:211453/151‑1 (MQ=255)
                          ccGCGTACCGGGAAAGGGGGCGGGCGTCCAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCC‑CACCGGTTCCCCCGCGCCGGGGGATgagc                                                                                                                              >  4:1124968/1‑150 (MQ=255)
                           cgcgTACCGGGAAAGGGGGCGGGCGTCCAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGGG‑CCCGCGGTGCCGCCCGCCggggggtgggcc                                                                                                                             >  5:395169/1‑142 (MQ=255)
                           cgcgTACCGGGAAAGGGGGCGGGCGTCCAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑CCCGGGTTGCCGCGCGCCggggggtcagcc                                                                                                                             >  6:1407092/1‑142 (MQ=255)
                              gTACCGGGAAAGGGGGCGGGCGTCCAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑CACCGGTTCCCCCGCCGCGGGtgctgccccgcc                                                                                                                          >  5:107535/1‑144 (MQ=255)
                                aCCGGGAAAGGGGGCGGGCGTCCAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑GCCGGTTTGCCCCGCGCCGGGGGCTGCGCCGCCgg                                                                                                                        >  1:1279783/1‑151 (MQ=255)
                                 ccGGGAAAGGGGGCGGGCGTCCAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑CACGGTTTCCCGCGCGCCGGGTGATGAGACGCCGGa                                                                                                                       <  8:438936/151‑1 (MQ=255)
                                 ccGGGAAAGGGGGCGGGCGTCCAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑CACGGTTTCCCGCGCGCCGGGTGATGAGACGCCGGa                                                                                                                       <  7:309499/151‑1 (MQ=255)
                                    ggAAAGGGGGCGGGCGTCCAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑CACGGTTTCCCGCGCGCCGGGTGATGAGACGCCGGaaaa                                                                                                                    <  2:934227/151‑1 (MQ=255)
                                       aaGGGGGCGGGCGTCCAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑CACGGTTTCCCGCGCGCCGGGTGATGAGACGCCGGAAAAGGc                                                                                                                 <  5:1123182/151‑1 (MQ=255)
                                         gggggCGGGCGTCCAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑CACGGTTTCCCGCGCGCCGGGTGATGAGACGCCGGAAAAGGCCg                                                                                                               <  7:941200/151‑1 (MQ=255)
                                          ggggCGGGCGTCCAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCGCCCCGGTTTCCC‑CCCGCCCGGGGAGGAGACCCCCGCAAACGcccc                                                                                                              >  8:93940/1‑149 (MQ=255)
                                            ggcgggcgTCCAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑CACGGTTTCCCGCGCGCCGGGTGATGAGACGCCGGAAAAGGCCGATc                                                                                                            <  2:146135/151‑1 (MQ=255)
                                              cgggcgTCCAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑GACGGTTTCCCGCGCGGCGGGTGCTGAGCCGCCGGAACAGGcccggcac                                                                                                          >  4:762912/1‑145 (MQ=255)
                                               gggcgTCCAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑CACGGTTTCCCGCGCGCCGGGTGATGAGACGCCGGAAAAGGCCGATCACg                                                                                                         <  1:1405535/151‑1 (MQ=255)
                                               gggcgTCCAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑CACGGTTTCCCGCGCGCCGGGTGATGAGACGCCGGAAAAGGCCGATCACg                                                                                                         <  2:1382439/151‑1 (MQ=255)
                                                 gcgTCCAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑CACGGTTTCCCGCGCGCCGGGTGATGAGACGCCGGAAAAGGCCGATCACGGt                                                                                                       <  4:411354/151‑1 (MQ=255)
                                                 gcgTCCAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑CACGGTTTCCCGCGCGCCGGGTGATGAGACGCCGGAAAAGGCCGATCACGGt                                                                                                       <  1:82208/151‑1 (MQ=255)
                                                  cgTCCAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑GCCGGGTTGCCCCCCGCCGGGGGGTGCGCCGCCCGCCAAGGCCgcgccccggg                                                                                                      >  1:220723/1‑142 (MQ=255)
                                                  cgTCCAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑CACGGTTTCCCGCGCGCCGGGTGATGAGACGCCGGAAAAGGCCGATCACGGTg                                                                                                      <  7:668500/151‑1 (MQ=255)
                                                   gTCCAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑CCCGGGGTACCGCGCCCCGGGGGGGGAGCCCCCGGcaaacggcgcgcccggggg                                                                                                     >  8:496502/1‑136 (MQ=255)
                                                   gTCCAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑CCCCGGTTGCCGCGCGCCGGGGGCTGAGCCGCCCGCAAAGGCCGCTCCCGgggg                                                                                                     >  5:1213632/1‑151 (MQ=255)
                                                   gTCCAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑CACGGTTTCCCGCGCGGCGGGTGATGAGACGCCGGAAAAGGCCGATCACGGTgg                                                                                                     <  1:1096967/151‑1 (MQ=255)
                                                   gTCCAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑CACGGTTTCCCGCGCGCCGGGTGATGAGACGCCGGAAAAGGCCGATCACGGTgg                                                                                                     <  5:791052/151‑1 (MQ=255)
                                                    tCCAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑CCCCGTTTACCGCGCGCCCGGTGATGAGACCCCGGAAAAGGCCCCTCAAGGGGGc                                                                                                    >  6:884180/1‑151 (MQ=255)
                                                    tCCAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑CACGGTTTCCCGCGCGCCGGGGGATGAGACGCCGGAAAAGGCCGCTCCAGGTGGc                                                                                                    >  4:554788/1‑151 (MQ=255)
                                                      cAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑CCCCGTTTACCGCGCGCCGGGGGATGAGACGCCGGAAAAGGCCGATCACGGTGGGAt                                                                                                  >  6:550937/1‑151 (MQ=255)
                                                        ggAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑CACGGTTTCCCGCGCGCCGGGTGATGAGACGCCGGAAAAGGCCGATCACGGTGGCATCa                                                                                                <  5:1255718/151‑1 (MQ=255)
                                                          aaaaTCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑CCCGCGTTGACCCGCGCCCGGGGGTGTGGCGCCGGCAAACGCCGCACACGGGGGcctgacg                                                                                              >  8:752851/1‑145 (MQ=255)
                                                          aaaaTCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑CACGGTTTCCCGCGCGCCGGGTGATGAGACGCCGGAAAAGGCCGATCACGGTGGCATCAGt                                                                                              <  1:291567/151‑1 (MQ=255)
                                                           aaaTCTCGTGCTGACGGTGGCCGGCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑CACGGTTTCCCGCGCGCCGGGTGATGAGACGCCGGAAAAGGCCGATCACGGTGGCATCAGTg                                                                                             <  4:1339625/151‑1 (MQ=255)
                                                               gtcGTGCTGACGGTGGCCGTCGCCGCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑CACGGTTTCCCGCGCGCCGGGTGATGAGACGCCGGAAAACGCCGATCACGGTGGCATCAGTGG‑Ta                                                                                         <  3:486756/149‑1 (MQ=255)
                                                                 cGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑CACGGTTTCCCGCGCGCCGGGTGATGAGACGCCGGAAAAGGCCGATCACGGTGGCATCAGTGG‑Taaaa                                                                                      <  8:418769/151‑1 (MQ=255)
                                                                    gCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑CACGGTTTCCCGCGCGCCGGGTGATGAGACGCCGGAAAAGGCCGATCACGGTGGCATCAGTGG‑TAAAAGtt                                                                                   <  1:1178527/151‑1 (MQ=255)
                                                                      tGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGGC‑CCCCGGTTACCGCGCGCCCGGGGGTGAGACCCCGGGAAAAGCCGCGCACCGGGGGCTCAGGGG‑GAAAAAtttt                                                                                 >  4:679028/1‑151 (MQ=255)
                                                                       gACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑CACGGTTTCCCGCGCGCCGGGTGATGAGACGCCGGAAAAGGCCGATCACGGTGGCATCAGTGG‑TAAAAGtttt                                                                                 <  5:674569/150‑1 (MQ=255)
                                                                        aCGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGGG‑CACCGGTTACCCCGCCCCCGGGGAATACACGCCGGCAAAAGCAGAAAACCGGGGCCTCAGGGG‑GAAAAAGTTTAt                                                                               >  8:221408/1‑151 (MQ=255)
                                                                          ggTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑CACGGTTTCCCGCGCGCCGGGTGATGAGACGCCGGAAAAGGCCGATCACGGTGGCATCAGTGG‑TAAAAGTTTTATTg                                                                             <  7:1385100/151‑1 (MQ=255)
                                                                          ggTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑CACGGTTTCCCGCGCGCCGGGTGATGAGACGCCGGAAAAGGCCGATCACGGTGGCATCAGTGG‑TAAAAGTTTTATTg                                                                             <  2:841331/151‑1 (MQ=255)
                                                                           gTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑CACGGTTTCCCGCGCGCCGGGTGATGAGACGCCGGAAAAGGCCGATCACGGTGGCATCAGTGG‑TAAAAGTTTTATTGa                                                                            <  8:1419486/151‑1 (MQ=255)
                                                                           gTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑CACGGTTTCCCGCGCGCCGGGTGATGAGACGCCGGAAAAGGCCGATCACGGTGGCATCAGTGG‑TAAAAGTTTTATTGa                                                                            <  7:357542/151‑1 (MQ=255)
                                                                           gTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑CACAGTTTCCCGCGCGCCGGGTGATGAGACGCCGGAAAAGGCCGATCACGGTGGCATCAGTGG‑TAAAAGTTTTATTGa                                                                            <  8:1237078/151‑1 (MQ=255)
                                                                             ggCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCGCCCCGGTTTCCC‑CGCGGCCGGGGGTGAGACCCCGGCCAAGGCCGCGCACGGGGGGCTGCGTGGTTAAAAGTttttttatc                                                                           >  8:1110572/1‑148 (MQ=255)
                                                                             ggCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑CACGGTTTCCCGCGCGCCGGGTGATGAGACGCCGGAAAAGGCCGATCACGGTGGCATCAGTGG‑TAAAAGTTTTATTGATc                                                                          <  1:933544/151‑1 (MQ=255)
                                                                               ccGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGGG‑CCCGGGTTGCCGCGCGCCGGGGGGTGGGGCGCCCGCAAACGCCGCGCACGGGGGGGTCAGGGG‑TTAAAGTtttttttttgcg                                                                        >  1:397870/1‑145 (MQ=255)
                                                                               ccGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑CACGGTTTCCCGCGCGCCGGGTGATGAGACGCCGGAAAAGGCCGATCACGGTGGCATCAGTGG‑TAAAAGTTTTATTGATCCg                                                                        <  1:1060821/151‑1 (MQ=255)
                                                                                cGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑CCCGGTTTCCCGCGCGCCGGGGGATGAGCCGCCGGCAAAGGCCGCTCACGGGGGGCTCAGTGG‑TAAAAGTTTTTTTGATCCgg                                                                       >  3:802981/1‑151 (MQ=255)
                                                                                 gTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑CCCCGGTTACCGCGCGCCGGGGGGTGAGACGCCGGGAAACGCCCCTCACGGGGGCCTCAGTGG‑GAAAACTTTTATTGATGCggg                                                                      >  7:1217889/1‑150 (MQ=255)
                                                                                   cgcccagcccGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGTAAGATTCTCATG‑TGCG‑AACTGTTTCCCGCGCGCCGGGTGATGAGACGCCTGAAAAGGCCGATCACGGTTGCATCAGTTG‑TAAAAGTTTTATTGATCCGGaa                                                                     <  6:353130/150‑1 (MQ=255)
                                                                                    gccccgcccGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑CACGGTTTCCCGCGCGCCGGGTGGTGAGACGCCGGCAAAGGCCGCTCACCGGGGCATCAGTGG‑TAAAAGGTTTATTGATCCGGTAgt                                                                   >  3:838093/1‑151 (MQ=255)
                                                                                    gccccgcccGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑CACGGTTTCCCGCGCGCCGGGTGATGAGACGCCGGAAAAGGCCGATCACGGTGGCATCAGTGG‑TAAAAGTTTTATTGATCCGGAAgt                                                                   <  3:326227/151‑1 (MQ=255)
                                                                                    gccccgcccGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑CACGGTTTACCCCGCGCCGGGGGATGAGACGCCGGAAAAGGCCGCTCACGGTGGCATCACGGG‑TTAAACTTTTATTGATCCGGaact                                                                   >  1:778983/1‑149 (MQ=255)
                                                                                       ccgcccgcGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑CACGGTTTCCCGCGCGCCGGGTGATGAGACGCCGGAAAAGGCCGATCACGGTGGCATCAGTGG‑TAAAAGTTTTATTGATCCGGAAGTGtt                                                                <  5:360659/151‑1 (MQ=255)
                                                                                         gcccgcGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑CACGGTTTCCCGCGCGCCGGGTGATGAGACGCCGGAAAAGGCCGATCACGGTGGCATCAGTGG‑TAAAAGTTTTATTGATCCGGAAGTGTTGc                                                              <  3:167608/151‑1 (MQ=255)
                                                                                         gcccgcGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCC‑CCCCGTTTACCGCCCCCCCCGGGATAAAACCCCCGCAAAAGACCATACAGGTGGCATCAGTTG‑TTAAAATTTTTTTGATGGGGAAATGtttg                                                              >  2:663462/1‑149 (MQ=255)
                                                                                          cccgcGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑CACGGTTTCCCGCGCGCCGGGTGATGAGACGCCGGAAAAGGCCGATCACGGTGGCATCAGTGG‑TAAAAGTTTTATTGATCCGGAAGTGTTGcg                                                             <  7:1148456/151‑1 (MQ=255)
                                                                                            cgcgATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑CCCGGGTTGCCCCGCGCCGGGGGATGGGACGCCGGCAAAGGCCGCTCCCGGTGGGCTGAGGGG‑GAAAAGTTTTTTTGATCCGGAAGTGtttgggg                                                           >  3:1284624/1‑146 (MQ=255)
                                                                                              cgATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑CCCGGTTTTCCGCGCGCCGGGGGATGATCCGCCGGGGAAGGCCGCTCACGGGGGGCTGAGTGG‑GTAAAGTTTTTTTTGTTCGGTAGTGTTTggtcgg                                                         >  5:142497/1‑150 (MQ=255)
                                                                                                aTGACAAACAGAGTCAGCCTCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCT‑CTGGGTTTCGGGCGCGTGTGGTGATGAGACGCCGGATTAGGCCGATCTCGGTGGGTTCAGTGG‑TAAAATTTTTATTGATCCGGAAGTGTTGCGTCGTCa                                                       <  4:1074324/151‑1 (MQ=255)
                                                                                                aTGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGGG‑CACCGTTTGACCCGCGCCCGGGGGTTAGACCCCCGCAAAAGCCGCGCACCGGGGCATAAGAGG‑TTAAAATTTTATTGTTACGGGAGGGTTggggtgta                                                        >  6:255399/1‑143 (MQ=255)
                                                                                                  gACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑CACGGTTTCCCGCGCGCCGGGTGATGAGACGCCGGAAAAGGCCGATCACGGTGGCATCAGTGG‑TAAAAGCTTTATTGATCCGGAAGTGTTGCGTCGTCAGc                                                     <  3:1026451/151‑1 (MQ=255)
                                                                                                   aCAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑CACGCTTTACCGCGCGCCCGGTGGTGAGACGCCGGCAAAAGGCGATCACCGTGGCATCACTGG‑TAAAAATTTTTTTTATACGGAACTGTTTGGTCGTCAGCg                                                    >  2:1151249/1‑151 (MQ=255)
                                                                                                     aaaCAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑CACGGTTTCCCGCGCGCCGGGTGATGAGACGCCGGAAAAGGCCGATCACGGTGGCATCAGTGG‑TAAAAGTTTTATTGATCCGGAAGTGTTGCGTCGTCAGCGTg                                                  <  6:693595/151‑1 (MQ=255)
                                                                                                        cAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑CACGGTTTCCCGCGCGCCGGGTGATGAGACGCCGGAAAAGGCCGATCACGGTGGCATCAGTGG‑TAAAAGTTTTATTGATCCGGAAGTGTTGCGTCGTCAGCGTGCgg                                               <  4:152351/151‑1 (MQ=255)
                                                                                                            gTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑CACGGTTTCCCGCGCGCCGGGTGATGAGACGCCGGAAAAGGCCGATCACGGTGGCATCAGTGG‑TAAAAGTTTTATTGATCCGGAAGTGTTGCGTCGTCAGCGTGCGgaaga                                           <  5:255807/151‑1 (MQ=255)
                                                                                                            gTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑CACGGTTTCCCGCGCGCCGGGTGATGAGACGCCGGAAAAGGCCGATCACGGTGGCATCAGTGG‑TAAAAGTTTTATTGATCCGGAAGTGTTGCGTCGTCAGCGTGCGgaaga                                           <  5:619013/151‑1 (MQ=255)
                                                                                                             tCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑CACGGTTTCCCGCGCGCCCGGGGATGAGACCCCGGAAAAGGCCGCTCACGGTGGCCTCAGTGG‑GTAAAGTTTTATTTAAGCGGAAGTGTTGCGGCGGCAGCGTGCGgcagc                                           >  6:125945/1‑149 (MQ=255)
                                                                                                              cAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑CACCGTTTGCCGCGCGCCGGGGGATGAGACGCCGGAAAAGGCCGCTCACGGGGGGATCAGTGG‑TTAAATTTTTTTTTGTGCGGGAGTGTTTGGTGGTGAGCGTGGGggagaa                                          >  4:660860/1‑150 (MQ=255)
                                                                                                                 ccccGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑CACGGTTTCCCGCGCGCCGGGTGATGAGACGCCGGAAAAGGCCGATCACGGTGGCATCAGTGG‑TAAAAGTTTTATTGATCCGGAAGTGTTGCGTCGTCAGCGTGCGGAAGAAAccc                                      <  3:1167574/151‑1 (MQ=255)
                                                                                                                  cccGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑CCCGGGTTGCCCCGCGCCGGGTGGTGCGACGCCGGCAAAAGGCGCGGACCGGGGGGTGACTGG‑TAAAAGTTTTATTGATCCGGGAGTGTTGGGGCGGCAGCGTGCGgattcaaaccc                                     >  1:855105/1‑142 (MQ=255)
                                                                                                                   ccGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCGCCCCGGTTTGCC‑CGGGCCCGGGGCGGGGACGCCGGATAAGGCCGCTGACGGTGGCCGCAGGTG‑TGAAAATTTTTTTTTTCCGGGAGTTTTTAGGCGTGCCCGTGCGGCAGAAAccccc                                    >  3:899190/1‑149 (MQ=255)
                                                                                                                    cGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑CCCGGGTTGCCGCGCGCCCGGTGGTGAGCCGCCGGAAAAAGCCGCTCACGGGGGGCTCAGTGG‑GAAAAGTTTTTTTTATCCGGGAGTGGTTGGTGGTGAGCGGGGGGAAGCAACCcccg                                   >  1:340390/1‑151 (MQ=255)
                                                                                                                    cGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑CACGGTTTCCCGCGCGCCGGGTGATGAGACGCCGGAAAAGGCCGATCACGGTGGCATCAGTGG‑TAAAAGTTTTATTGATCCGGAAGTGTTGCGTCGTCAGCGTGCGGAAGAAACCcgcg                                   <  5:1027366/151‑1 (MQ=255)
                                                                                                                     gTGACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑CCCGGTTTCCCGCGCGCCCGGTGCTGAGACGCCCGAAAAGGCCGATGACGGGGGCATGACTGG‑TAAAAATTTTATTGATACGGAAGTGTTTCGTCGTCAGCGTGGGGGATAAAACcgcgg                                  >  4:1253870/1‑150 (MQ=255)
                                                                                                                       gACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑CACGGTTTCCCGCGCGCCGGGTGATGAGACGCCGGAAAAGGCCGATGACGGTGGCATCAGTGG‑TAAAAGTTTTATTGATCCGGAAGTGTTGCGTCGTCAGCGTGCGGAAGAAACCCGCGTCt                                <  8:461381/151‑1 (MQ=255)
                                                                                                                       gACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑CACGGTTTCCCGCGCGCCGGGTGATGAGACGCCGGAAAAGGCCGATCACGGTGGCATCAGTGG‑TAAAAGTTTTATTGATCCGGAAGTGTTGCGTCGTCAGCGTGCGGAAGAAACCCGCGTCt                                <  1:1026313/151‑1 (MQ=255)
                                                                                                                          cGCAAGTGGGAAGATTCGCCGT‑GGCG‑CACGGTTTCCCGCGCGCCGGGTGATGAGACGCCGGAAAAGGCCGATCACGGTGGCATCAGTGG‑TAAAAGTTTTATTGATCC‑‑AAGTGTTGCGTCGTCAGCGTGCGGAAGAAACCCGCGTCTACGGc                           <  2:351654/151‑1 (MQ=255)
                                                                                                                               gTGGGAAGATTCGCCGT‑GGCG‑CACGGTTTCCCGCGCGCCGGGTGATGAGACGCCGGAAAAGGCCGATCACGGTGGCATCAGTGG‑TAAAAGTTTTATTGATCCGGAAGTGTTGCGTCGTCAGCGTGCGGAAGAAACCCGCGTCTACGGCGaa                        <  4:732749/151‑1 (MQ=255)
                                                                                                                                  ggAAGATTCGCCGT‑GGCG‑CACGGTTTCGCGCGCGCCGGGTGATGAGACGCCGGAAAAGGCCGATCACGGTGGCATCAGTGG‑TAAAAGTTTTATTGATCCGGAAGTGGTGCGTCGTCAGCGTGCGGAAGAAACCCGCGTCTACGGCGAAAAt                     <  1:545661/151‑1 (MQ=255)
                                                                                                                                     aGATTCGCCGT‑GGCG‑CCCCGTTTACCCCGCGCCGGGGGATGAGACCCCCGAAAAGGCCGATCACCGTGGCATCAGTGG‑TAAAAGTTTTATTTATACCGAAGTGTTGCGGCGTCAGCGTGC‑GCAGAAACCCCCCGCTACGGCGCAAAAGCAt                 >  4:1057853/1‑151 (MQ=255)
                                                                                                                                     aGATTCGCCGT‑GGCG‑CACGGTTTCCCGCGCGCCGGGTGATGAGACGCCGGAAAAGGCCGATCACGGTGGCATCAGTGG‑TAAAAGTTTTATTGATCCGGAAGTGTTGCGTCGTCAGCGTGCGGAAGAAACCCGCGTCTACGGCGAAAATGCa                  <  6:1480180/151‑1 (MQ=255)
                                                                                                                                            ccGT‑GGCG‑CCCCGTTTACCGCGCCCCGGGTGATGAGACCCCCGAAAACGCCGATCACGGTGGCCTCAGTGG‑TAAAAGTTTTATTTATCCGGAAATGTTTCGTCGTCAGCGTGCGGAAGAAACCCGCGTCTACGGCGAAAATGGATGTGAgg           >  8:795062/1‑151 (MQ=255)
                                                                                                                                             cGT‑GGCG‑CACGGTTTCCCGCGCGCCGGGTGATGAGACGCCGGAAAAGGCCGATCACGGTGGCATCAGTGG‑TAAAAGTTTTATTGATCCGGAAGTGTTGCGTCGTCAGCGTGCGGAAGAAACCCGCGTCTACGGCGAAAATGCATGTCAGGc          <  7:508873/151‑1 (MQ=255)
                                                                                                                                              gT‑GGCG‑CACGGTTTCCCGCGCGCCGGGTGATGAGACGCCGGAAAAGGCCGATCACGGTGGCATCAGTGG‑TAAAAGTTTTATTGATCCGGAAGTGTTGCGTCGTCAGCGTGCGGAAGAAACCCGCGTCTCCGGCGAAAATGCATGTCAGGcc         >  5:163145/1‑150 (MQ=255)
                                                                                                                                                  gcg‑cACGGTTTCCCGCGCGCCGGGTGATGAGACGCCGGAAAAGGCCGATCACGGTGGCATCAGTGG‑TAAAAGTTTTATTGATCCGGAAGTGTTGCGTCGTCAGCGTGCGGAAGAAACCCGCGTCTACGGCGAAAATGCATGTCAGGCACtt      <  5:986595/151‑1 (MQ=255)
                                                                                                                                                    g‑cACGGTTTCCCGCGCGCCGGGTGATGAGACGCCGGAAAAGGCCGATCACGGTGGCATCAGTGG‑TAAAAGTTTTATTGATCCGGAAGTGTTGCGTCGTCAGCGTGCGGAAGAAACCCGCGTCTACGGCGAAAATGCATGTCAGGCACtttt    >  2:751770/1‑151 (MQ=255)
                                                                                                                                                    g‑cACGGTTTCCCGCGCGCCGGGTGATGAGACGCCGGAAAAGGCCGATCACGGTGGCATCAGTGG‑TAAAAGTTTTATTGATCCGGAAGTGTTGCGTCGTCAGCGTGCGGAAGAAACCCGCGTCTACGGCGAAAATGCATGTCAGGCACtttt    <  2:1228160/151‑1 (MQ=255)
                                                                                                                                                      cACGGTTTCCCGCGCGCCGGGTGATGAGACGCCGGAAAAGGCCGATCACGGTGGCATCAGTGG‑TAAAAGTTTTATTGATCCGGAAGTGTTGCGTCGTCAGCGTGCGGAAGAAACCCGCGTCTACGGCGAAAATGCATGTCAGGCACTTTTc   <  1:1458662/151‑1 (MQ=255)
                                                                                                                                                       |                                                                                                                                                       
ATGATTCTGATAAACGTACCCACAACCCGCGTACCGGGAAAGGGGGCGGGCGTCCAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATGACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGT‑GGCG‑CACGGTTTCCCGCGCGCCGGGTGATGAGACGCCGGAAAAGGCCGATCACGGTGGCATCAGTGG‑TAAAAGTTTTATTGATCCGGAAGTGTTGCGTCGTCAGCGTGCGGAAGAAACCCGCGTCTACGGCGAAAATGCATGTCAGGCACTTTTCC  >  DH1/1165295‑1165594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑