Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH12,591,5130TG24.9% 179.5 / 37.1 209D86A (GAC→GCC) ECDH1_RS12815hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (72/55);  new base G (13/29);  total (100/109)
Fisher's exact test for biased strand distribution p-value = 4.43e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 211 total aligned reads displayed.

CCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAACGAATCCGCAATCAAATATTCTGCCCAAGCGGGGAAGGATG  >  DH1/2591366‑2591660
                                                                                                                                                   |                                                                                                                                                   
            cgacttcgatggttctAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTATCAATCGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGt                                                                                                                                       <  4:993507/135‑1 (MQ=11)
                    cacgtaggaaTCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTCCCAACCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGGCAGCGAGAGAGCGTTGCCGCAgg                                                                                                                              <  5:1123017/143‑1 (MQ=11)
                      gatattAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTCTCAATCGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCa                                                                                                                            <  8:429074/146‑1 (MQ=255)
                      cTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGACCGTTGCCGCAGGc                                                                                                                             >  1:266724/1‑150 (MQ=255)
                        tactatctcTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTGTCAATCGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGc                                                                                                                             <  6:1051285/140‑1 (MQ=14)
                        gtctAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTCGCAACCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAAc                                                                                                                          <  3:450084/148‑1 (MQ=255)
                            aaTCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGAcc                                                                                                                      <  1:1175435/151‑1 (MQ=255)
                            aaTCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGGCAGCGAGAGAGCGTTGCCGCAGGCAACGAcc                                                                                                                      <  1:1007392/151‑1 (MQ=255)
                            aaTCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTATCAAGCGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGAc                                                                                                                       <  6:375809/150‑1 (MQ=255)
                                 ttCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTAGAGCGACAGCGAGAGAGCGTTGCCGCAGGCAACGAcc                                                                                                                      >  7:268122/1‑146 (MQ=255)
                                  tCCCCCACCACCATCACTTTCAAAAGTCCCTCACCTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAg                                                                                                                 <  1:139496/150‑1 (MQ=255)
                                    ccccAGCTCCATCACTTTCAAAAGTCCCTGAACTCACAACCGAATCCGCAATAAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGc                                                                                                              <  7:700204/151‑1 (MQ=255)
                                     cccACCACAATCACTTTCAAAAGTCCCTGAACTTTCAATCGAATCCGCAATAAAATATTCTGCCCATGCGGGGAAGTATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCg                                                                                                             <  8:136934/151‑1 (MQ=255)
                                       caccaccaTCACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGa                                                                                                            >  7:325145/1‑150 (MQ=255)
                                        accaccaTCACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGa                                                                                                            >  4:1170302/1‑149 (MQ=255)
                                         cctccaTCACTTTAAAAATTCCCTGAAGTTTCTAGAGAATCGTCAATTAATTTTTGTGCCCATGGGGGGAAGTATGAGAAGGTTGGAGTATGGTTCGGCTCGAGGGTCAGGGAGTGAGCGTTGCGGCTGGCAACGACCCGAAGGGCGAAgc                                                                                                         <  8:377064/148‑1 (MQ=2)
                                         ccaccaTCACTTTCAAAAGTCCCTGAACTCTCAAGCCAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACGAAGGTTCGGCTCGAGCGGCAGCGAGAGAGCGTTCCCGCAGGCAACGACCCGAAGGGCGAAgc                                                                                                         >  3:584558/1‑151 (MQ=255)
                                         acaccaTCATTTTAAAAAGTCCCTGAACTTTCAATCTAATCCGAAATTAAATATTCTGCCCATGCGGGTAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAgc                                                                                                         <  8:202096/150‑1 (MQ=11)
                                           accaTCACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAgcgc                                                                                                       >  2:119096/1‑151 (MQ=255)
                                           accaTCACTTTCAAAAGGCCCTGAACTCCCAACCCAATCCGCAATAAAATATGCTGCCCATGCGGGGAAGGATGAGAAGCGTCGACTAAGGTGCGGCGCGAGCGCCAGCGAGAGAGCGTGGCCGCAGGCAACGACCCGAAGGGCGAAgcgc                                                                                                       <  3:934798/151‑1 (MQ=11)
                                                 aCTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAgcg                                                                                                 >  1:21021/1‑151 (MQ=255)
                                                   tttCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTACGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCt                                                                                               >  6:742826/1‑151 (MQ=255)
                                                      cAAAAGTCCCTGAACTCTCAACCGAATCCGCAATAAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGa                                                                                             <  2:151104/150‑1 (MQ=255)
                                                      aaaaaGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACAAAGGTTCGACTTGAGCGCCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAg                                                                                            <  4:193703/150‑1 (MQ=255)
                                                       aaaaGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGt                                                                                           <  7:1445671/151‑1 (MQ=255)
                                                       aaaaGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGGCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGt                                                                                           >  3:588334/1‑151 (MQ=255)
                                                        aaaGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGt                                                                                           >  3:1061186/1‑150 (MQ=255)
                                                        aaaGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACAAAGGTTCGACTAGAGCGCCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTg                                                                                              >  8:586108/1‑147 (MQ=255)
                                                          aGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAAt                                                                                        >  4:835678/1‑151 (MQ=255)
                                                             cccTGAAGTCTCAATCGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCt                                                                                     <  4:219975/151‑1 (MQ=255)
                                                             cccTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACAAAGGTTCGACTTGAGCGCCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCt                                                                                     <  4:590220/151‑1 (MQ=9)
                                                             cccTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATCCGGGTAAGGATGAGACCATACGAAAAAGTTACGCATGGACCTCAACCGAAAAACCTTTGCCGCAGGCAACGCCCCGAAGGCCAAACCCCCCACCCCTGAGTAATcat                                                                                     >  8:232928/1‑149 (MQ=2)
                                                              ccTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCtt                                                                                    >  3:483445/1‑151 (MQ=255)
                                                              ccTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGGCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCtt                                                                                    >  7:1383307/1‑151 (MQ=255)
                                                               cTGAACTCTCAAGCGAATCCGCAGTCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACGAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAgctcggaagagaggagg                                                                                   >  4:1128666/1‑136 (MQ=14)
                                                               cTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTc                                                                                   >  4:935840/1‑151 (MQ=255)
                                                               cTGAACTCTCAAGCGAATCCGCAATCAAAAATTCTCCCCATCCGGGGAAGGATGAGAAGCTTCGACTAAGCTTCGCCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCCCGCAGCGCTGACTACTCCtac                                                                                   >  7:785287/1‑149 (MQ=14)
                                                                 gacctccCACGCGAAGCCGCAATAAAATATGCTCCCCATGCGGGGACGGATGAGAAGCTTCGACTACGGTTCGGCTCGACCGCCCGCGAGAGAGCGTTGCCGCAGGAAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTcc                                                                                  <  7:912693/147‑1 (MQ=11)
                                                                  aaCTCCCAACCGAATCCGCAATAAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGTCTCGAGCGTCAGCGAGAGAGCGTTGCCTCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTcccc                                                                                <  6:1183777/151‑1 (MQ=255)
                                                                   aCTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTccccc                                                                               >  1:549459/1‑151 (MQ=255)
                                                                   aCTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGGCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTccccc                                                                               >  6:1406837/1‑151 (MQ=255)
                                                                   aCTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGACTTGAGCGCCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAgcg                                                                                                        <  6:946921/126‑1 (MQ=9)
                                                                   aCTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACGAAGGTTCGGCTGGAGCGGCAGCGAGAGAGCGTTGCCGCAGGCAAc                                                                                                                          >  5:462199/1‑108 (MQ=255)
                                                                    ctctCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTccccc                                                                               >  4:734755/1‑150 (MQ=255)
                                                                    ctctCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACAAAGGTTCGGCTCGAGCGACAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTccccc                                                                               >  4:1254853/1‑150 (MQ=255)
                                                                     tctcAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCccac                                                                             >  8:497843/1‑151 (MQ=255)
                                                                     tctcAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCccac                                                                             >  7:600412/1‑151 (MQ=255)
                                                                     tctcAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACAAAGGTTCGGCTAGAGCGTCAGCGAGAGAGCGTTCCCCCAGGCAACGACCCGAAGGGCGAACCGCGCAGCGCTGACTAATCCTTCCCccac                                                                             >  5:1407542/1‑151 (MQ=14)
                                                                      ctcAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCccacc                                                                            >  4:459938/1‑151 (MQ=255)
                                                                        ccaacGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCccaccac                                                                          <  7:1466335/147‑1 (MQ=25)
                                                                        cAATCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGACTGGAGCGGCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCccacc                                                                            <  2:20952/149‑1 (MQ=9)
                                                                        cAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGTCTTGAGCGGCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCccacca                                                                           <  4:285857/150‑1 (MQ=16)
                                                                          aGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTAGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCccaccacc                                                                         >  4:626138/1‑150 (MQ=255)
                                                                            cGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATc                                                                      <  1:1054929/151‑1 (MQ=18)
                                                                             gAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACGAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCt                                                                                     >  6:559001/1‑135 (MQ=37)
                                                                             caaTCCGCAATAAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTACGGTTCGGCTCGAGCGCCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCa                                                                     <  7:93444/150‑1 (MQ=9)
                                                                              aaTCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGTCTTGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATcg                                                                     <  2:1381306/150‑2 (MQ=18)
                                                                               aTCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCGCt                                                                   >  2:378421/1‑151 (MQ=38)
                                                                                tCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCGCtt                                                                  >  2:523836/1‑151 (MQ=38)
                                                                                   gCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCCCttcc                                                                >  6:880057/1‑148 (MQ=255)
                                                                                     aaTTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGCCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCaag                                                             <  2:1297202/151‑2 (MQ=14)
                                                                                      aTCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCGCttgctta                                                             >  3:1112362/1‑145 (MQ=255)
                                                                                        cAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCCCCACCATCGCttgcttaaa                                                           >  7:442839/1‑143 (MQ=255)
                                                                                             atTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCgg                                                     <  6:171999/151‑1 (MQ=39)
                                                                                             atTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGCCTCGAGCGGCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCg                                                      <  3:597294/150‑1 (MQ=21)
                                                                                               tCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACATACAAAGGcccacga                                                    >  2:988404/1‑146 (MQ=255)
                                                                                                  gCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCAt                                                                       >  5:841068/1‑128 (MQ=37)
                                                                                                  gCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGCCTCGAGCGGCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGaa                                                   <  1:82012/148‑1 (MQ=25)
                                                                                                  gCCCATGCGGGGAAGGATGAGAAGCTTCGACAAAGGTGCGCCTCGAGCGGCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAAtt                                                 <  3:1436332/150‑1 (MQ=255)
                                                                                                  gCCCATGCGGGGAAGGATGAGAAGCTTCGACAAAGGTGCGCCTCGAGCGCCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGaa                                                   <  1:1082328/148‑1 (MQ=9)
                                                                                                   ggcATGCGGGGAAGTATGAGAAGCTGCGACAAATGTTCGGCTCGAGCGGCCGCGAGAGTGGGTTGCGGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATtct                                               <  5:617330/149‑1 (MQ=14)
                                                                                                   cccATGCGGGTAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATtct                                               <  6:1299345/151‑1 (MQ=255)
                                                                                                   cccATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGTCTTGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATcgc                                                                    <  2:922149/130‑3 (MQ=16)
                                                                                                     cATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCCCTTTCAAAAGCCCCGGAATtctc                                              >  7:804357/1‑150 (MQ=255)
                                                                                                     cATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCCCTTCCataactaatccaatcctgt                                             >  7:1045575/1‑136 (MQ=11)
                                                                                                        gtgttgatttaTGAGAAGCTTCGACTAAGGTTCGTCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAAc                                          <  8:746847/141‑1 (MQ=255)
                                                                                                        gCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGCCTCGAGCGGCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCaaa                                           <  3:849306/150‑1 (MQ=255)
                                                                                                        gCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGCCTCGAGCGCCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAAc                                          <  1:523826/151‑1 (MQ=255)
                                                                                                         cGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTAGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGa                                                                                             >  3:1321298/1‑99 (MQ=255)
                                                                                                            gtaggtattaaaaGCTTCCACTACGGTGCGCCGCGCGGGCCAGCGAGAGGGGGTGGCCGCTGCCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAACGAAt                                      <  7:1305803/140‑1 (MQ=14)
                                                                                                              aaGGATGAGAAGCTTCGACTAAGGTTCGTCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAACGAATcc                                    <  4:1317521/151‑1 (MQ=255)
                                                                                                              aaGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCTAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAACGAATcc                                    <  8:697341/151‑1 (MQ=255)
                                                                                                               aGGATGAGAAGCTTCGACTAAGGTTCGGCTGGTGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAACGAATCCg                                   <  4:66251/151‑1 (MQ=255)
                                                                                                               aGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAACGAATCCg                                   <  5:742704/151‑1 (MQ=255)
                                                                                                                ggATGAGAAGCTTCGACTAAGGTTCGTCTCGAGCGTAAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAACGAATCCg                                   <  6:604256/150‑1 (MQ=255)
                                                                                                                     agaAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAACGAATCCGCAATc                              >  3:227485/1‑150 (MQ=255)
                                                                                                                     agaAGCTTCGACGAAGGTTCGCCTCGAGCGGCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAACGAATCCGCAATc                              <  7:484606/150‑1 (MQ=255)
                                                                                                                         gctgccaccaaGGTTCGCCTCGAGCGCCAGCGAGAGAGCGTTGCCGCAGCCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAACGAATCCGCAATCAAata                         <  5:549458/142‑1 (MQ=11)
                                                                                                                           ttCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTACACCCCCACCATCACTTTCAAAAGCCACGGAATTCTCAAACTAATCCGCAATCAAATAtt                       >  3:288139/1‑151 (MQ=255)
                                                                                                                            ttcccttctgTTCGACTCGAGCGCCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAACGAATCCGCAATCAAATAtt                       <  6:1334513/141‑1 (MQ=255)
                                                                                                                            tCGACTAAGGTTCGTCTCGAGCGGCAGCGATAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAACGAATCCGCAATCAAATAtt                       <  6:280880/150‑1 (MQ=255)
                                                                                                                            tCGACCAAGGTTCGCCTCGAGCGCCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAACGAATCCGCAATCAAATAtt                       <  8:920673/150‑1 (MQ=16)
                                                                                                                              gACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCAACCCAAATCTCAAACGACTCCCaaataaaataatatg                    >  3:1214880/1‑146 (MQ=14)
                                                                                                                                  aaGGTCCGGCGCGCCCGCCAGCGAGAGCGCGTGGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCATCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAACGAATCCGCAATCAAATATTCTGCCCa                <  4:714463/151‑1 (MQ=14)
                                                                                                                                   aggcgcgccgcGAGCCCCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAATCTCGCAGCGCTGAGTAATCCTTCCCCCCCCACCATCACTTTCAAAAGCCCCGGACTTCTCAAACGAATCCGCAATCAAATATTCTGCCCaa               <  5:338193/141‑1 (MQ=11)
                                                                                                                                     ccgcGGTTCGAGCGCCAGCGAGAGAGCGTTGCCGCAGGAAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGGCCCGGAATTCTCAAACGAATCCGCAATCAAATATTCTGCCCAAGc             <  3:1167257/148‑1 (MQ=255)
                                                                                                                                       tcgtgtCGAGCGTCTGCTATAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCTGAATTCTCAAACGAATCCGCAATCAAATATTCTGCCCAAGCg            <  2:1218171/145‑1 (MQ=14)
                                                                                                                                       tCGACTCGAGCGCCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAACGAATCCGCAATCAAATATTCTGCCCAAGCg            <  2:369587/150‑1 (MQ=25)
                                                                                                                                         gacTCGAGCGCCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAAt                                                  >  3:1404255/3‑110 (MQ=14)
                                                                                                                                                gCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAACGAATCCGCAATCAAATATTCTGCCCAAGCGGGGAAGGATg  >  3:368421/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAACGAATCCGCAATCAAATATTCTGCCCAAGCGGGGAAGGATG  >  DH1/2591366‑2591660

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑