Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH13,447,4030AC25.9% 523.6 / 66.5 287G346G (GGT→GGG) malZalpha‑glycosidase
Reads supporting (aligned to +/- strand):  ref base A (63/149);  new base C (74/0);  total (137/150)
Fisher's exact test for biased strand distribution p-value = 1.37e-30
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 294 total aligned reads displayed.

CACGATAGTTCATGGCGGCATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATA‑TGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTCGGCAAGCTGGCA  >  DH1/3447255‑3447553
                                                                                                                                                    |                                                                                                                                                        
    aTAGTTCATGGCGGCATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCAcccccc                                                                                                                                                    >  8:448708/1‑151 (MQ=255)
     tAGTTCATGGCGGCATCTTCCACATCCGCCTGTAACCATTGACGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGTCTGGGATTCTTTCGACGATTCAGTGATCCAGGCAAAGTGATGAATATTATTGAGAGACCCaaacaca                                                                                                                                                    >  6:1329116/1‑144 (MQ=255)
      aGTTCATGGCGGCATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTGCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑Tc                                                                                                                                                 <  1:333756/151‑1 (MQ=255)
        ttCATGGCGGCATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑Tccc                                                                                                                                               >  4:1124077/1‑151 (MQ=255)
              gcggcATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCC‑TCCCCCAGcc                                                                                                                                         >  1:1117196/1‑150 (MQ=255)
               cggcATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCCCC‑Cacccccccc                                                                                                                                         >  8:305958/1‑146 (MQ=255)
               cggcATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCCCC‑CCCCCCaccc                                                                                                                                         >  7:687/1‑147 (MQ=255)
                      ttCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATA‑TGcacc                                                                                                                                >  7:844031/1‑151 (MQ=255)
                       tCCACATCGGCCTGTAACCATTGCCGTGCAGCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATA‑TGc                                                                                                                                   <  1:1193649/147‑1 (MQ=255)
                        ccACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATA‑TGcaccac                                                                                                                              <  2:292280/151‑1 (MQ=255)
                              cGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCC‑TCCCCCAGCATA‑TGCACCACATcc                                                                                                                          >  3:626146/1‑149 (MQ=255)
                              cGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATA‑TGCACCACATcca                                                                                                                         <  4:675185/150‑1 (MQ=255)
                              cGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATA‑TGCACCACATcca                                                                                                                         <  5:869375/150‑1 (MQ=255)
                              cGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTGCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATA‑TGCACCACATcca                                                                                                                         <  4:1191570/150‑1 (MQ=255)
                               ggCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCC‑TCCCCCAGCATA‑TGCACCACATccagc                                                                                                                       >  1:649951/1‑151 (MQ=255)
                                gCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATA‑TGCACCACATccagcc                                                                                                                      <  7:1261746/151‑1 (MQ=255)
                                gCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATA‑TGCACCACATccagcc                                                                                                                      <  4:1371735/151‑1 (MQ=255)
                                   tGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCC‑ACCCCCCGCATATTGCACC‑CAAacagccgcc                                                                                                                   >  6:292381/1‑151 (MQ=255)
                                      aaCCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCCCCGGCCCCCGGCA‑A‑TGGCCCACAAccacccgccccc                                                                                                                >  2:486885/1‑148 (MQ=255)
                                         cATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATA‑TGCACCACATCCAGCCGCCAGCCGt                                                                                                             <  8:523256/151‑1 (MQ=255)
                                             gCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATA‑TGCACCACATCCAGCCGCCAGCCGTCCat                                                                                                         <  5:498428/151‑1 (MQ=255)
                                               cGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATA‑TGCACCACATCCAGCCGCCAGCCGTCCatat                                                                                                       <  2:1059422/151‑1 (MQ=255)
                                               cGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑GCCCCCCGCATA‑TGCACCACCACCACCCGCCCGCCGTCCaaat                                                                                                       >  5:1295491/1‑151 (MQ=255)
                                               cGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑GCCCCCAGCATA‑TGCCCCACATCCAGCCGCCCGCCCTCCaaat                                                                                                       >  3:1426495/1‑151 (MQ=255)
                                                gTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCC‑GCCCCCCGCATA‑TGCACCACATCCCGCCGCCAGCCGTCCCTAtt                                                                                                      >  1:1037477/1‑151 (MQ=255)
                                                gTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATA‑TGCACCACATCCAGCCGCCAGCCGTCCATAtt                                                                                                      <  1:1400446/151‑1 (MQ=255)
                                                  gCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCC‑TCCCCCAGCATA‑TGCCCCACATCCAGCCGCCAGCCGTCCATATTcc                                                                                                    >  3:1249884/1‑151 (MQ=255)
                                                   cATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATA‑TGCACCACATCCAGCCGCCAGCCGTCCATATTCCa                                                                                                   <  4:26920/151‑1 (MQ=255)
                                                   cATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATA‑AGCACCACCTCCAGCCGCCCGCCGTCCATATTcc                                                                                                    >  3:316847/1‑150 (MQ=255)
                                                   cATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCCCC‑TCCCCCAGCATA‑TGCACCACATCCACCCGCCAGCCGTCCATATTccc                                                                                                   >  4:362267/1‑150 (MQ=255)
                                                   cATCGCCAAAATGCGCGCCGACAATATACGCTTCCGGCTGGGGTTCTTTCGCCGCTTCGGTGAGCCCGGCAACGGGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATA‑TGCACCACATCCAGCCGCCAGCCGTCCATATTCCa                                                                                                   <  2:1091825/151‑1 (MQ=255)
                                                       gCCAAAAGGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATA‑TGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGgc                                                                                               <  7:218006/151‑1 (MQ=255)
                                                        ccAAAATCTTCTCCGCCAATATACGCTTCCGGCTCGGTTTCTTTCCCCCCTTCGGTCATCCCCGCAACGTGCTGCATATTATTGCGCGCCCCACCCCCC‑ACCCCCAGCAAA‑ACCACCACAACAACCCCCCCCCCCTCCATAATcccccgcc                                                                                              >  2:1293904/1‑145 (MQ=255)
                                                          aaaaTGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑GCCCCCAGCATA‑TTGACCACATCCAGCCCCCAGCCGTGCCTATTCCACGGCGCt                                                                                            >  4:1004823/1‑151 (MQ=255)
                                                            aaTGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCC‑GCCCCCCGCATA‑TGCACCCCATCCAGCCGCCCGCCGTCCATATTCCACCGCGCgtt                                                                                          >  7:368266/1‑151 (MQ=255)
                                                            aaTGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATA‑TGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCttt                                                                                          <  3:387944/151‑1 (MQ=255)
                                                            aaTGTGCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATA‑TGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCttt                                                                                          <  7:536288/151‑1 (MQ=255)
                                                             aTGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGTGCCCCCCCCCCC‑GCCCCCACCATA‑TGGACCACCTCCCGCCGCCCCCCGGGCATAATCCACCgccgttt                                                                                          >  5:208508/1‑145 (MQ=255)
                                                             aTGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCCGCATA‑TGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCttt                                                                                          >  1:893030/1‑150 (MQ=255)
                                                             aTGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATA‑TGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTc                                                                                         <  1:214375/151‑1 (MQ=255)
                                                                   gCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATA‑TGCACCCCAACCACCCGCCACCCGTCCATATTCCACGGCGCTTTCAGCCac                                                                                   >  6:1338216/1‑150 (MQ=255)
                                                                     cGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCa                                                                                                                                         <  2:600658/96‑1 (MQ=255)
                                                                     cGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATA‑TGGACCACATCCAGCCGCCCGCCGTCCAAATTCCACGGCGCTTTCACCCAGTg                                                                                 >  6:1478131/1‑151 (MQ=255)
                                                                        cAATCTACGCCTCCGCCTGGGGTTCTTCCGCCGCTTCGGGGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATA‑TGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCg                                                                              <  6:714824/151‑1 (MQ=255)
                                                                          atatACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATA‑TGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGa                                                                            <  8:826504/151‑1 (MQ=255)
                                                                            ataCGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCC‑TCCCCCAGCATA‑TGCACCACCAACAGCCCCCCCCCCTGCATATTCCCCGGCGCGTTCAGCCCGTGGCgggc                                                                           >  2:751672/1‑148 (MQ=255)
                                                                              aCGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATA‑TGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACaa                                                                         <  5:79901/150‑1 (MQ=255)
                                                                               cGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATA‑TGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATa                                                                       <  8:36874/151‑1 (MQ=255)
                                                                                      ggCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATA‑TGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCtt                                                                <  8:1212181/151‑1 (MQ=255)
                                                                                       gCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCCTA‑TGCCCCACATCCAGCCGCCCGCCGGCCCTATTTCACGGCGCGTTCACCCAGTGGCCGGCAATACTGTCttg                                                               >  7:441737/1‑150 (MQ=255)
                                                                                            gTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCCCC‑TCCCCCAGCATA‑TGCACCACATCCACCCGCCCGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTACCcgc                                                          >  6:640492/1‑151 (MQ=255)
                                                                                             tttctttcGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGGC‑GCCCCCCGCAAA‑TGCCCCACATCCCGCCGCCAGCCGGCCCAATTACACCGCGCGTTGAGCCAGTGGCGGGCCATACTGTCTTCCCcgcg                                                         >  5:1414938/1‑151 (MQ=255)
                                                                                               tctttcGCCGCTTCGGTGGTCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATA‑TGCACCACCTCCAGCCGCCCGCCGTCCATATTCCACGGCGCGTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGAt                                                       >  2:886463/1‑151 (MQ=255)
                                                                                               tctttcGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCC‑TCCCCCCGCATA‑TGCCCCACAACCCGCCGCCCGCCGTCCATAATACAACGCGCATTCAGCCACTGGCGGACAATACTGTCTTGCCcgcgct                                                       >  8:1419025/1‑149 (MQ=255)
                                                                                                ctttcGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑ACCCCCAGCAAA‑AGCACCACAACCAGCCGCCAGCCCTCCATATTACACGGCGCTTTAAGCCAGTGGCCGACAATACTTTCTTCCCCGCGAt                                                       >  4:832261/1‑150 (MQ=255)
                                                                                                ctttcGCCGCTTCGGTGATCCCGCCAACGTGCTCCATATTATTGCGCGCCCCCCCCCCC‑GCCCCCAGCATA‑TGCACCACATCCAGCCGCCCGCCGTGCATTTTCCACGGCGCGTTCGGCCAGTGGCCGTCAATACTGGCTTCGCAGCGAt                                                       >  4:1065096/1‑150 (MQ=255)
                                                                                                  ttcGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATA‑TGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGAt                                                       <  7:692185/148‑1 (MQ=255)
                                                                                                  ttcGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATA‑TGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGAt                                                       <  1:1001526/148‑1 (MQ=255)
                                                                                                  gacgccgcTTCCGTGATCCCGGCAACGTGCGCCCTATTCTTGCGCGCCCCACCCGCC‑TCCCCCAGCATA‑TGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGGCATCCCCGCGATaa                                                     <  6:905127/148‑1 (MQ=255)
                                                                                                   tcGCCGCTTCGGGGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATA‑TGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATaaa                                                    <  2:406426/150‑1 (MQ=255)
                                                                                                    cgccgcTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATA‑TGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAAt                                                   <  6:756701/150‑1 (MQ=255)
                                                                                                      ccgcTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATA‑TGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATttc                                                <  3:460790/151‑1 (MQ=255)
                                                                                                      ccgcTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATA‑TGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATttc                                                <  6:1141358/151‑1 (MQ=255)
                                                                                                        gcTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATA‑TGCACCCCATCCAGCCGCCAGCCGTACATATTACACCGCGCTTTAAGCCAGTGGCGGCCAATAATGTCGTCCCCCCGAAAAATttaat                                              >  6:144825/1‑151 (MQ=255)
                                                                                                        gcTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATA‑TGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATttcat                                              <  8:995892/151‑1 (MQ=255)
                                                                                                        gcTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATA‑TGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATttcat                                              <  1:393686/151‑1 (MQ=255)
                                                                                                            cGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCC‑TCCCCCACCATA‑TGCACCACATACACCCGCCAGCCCTCCAAATTACACGGCGCTTTCACCCAGTGGCGGGCAATAAGGTCGTGCCCGCGATAAATTTAATTGAc                                          >  5:1234494/1‑151 (MQ=255)
                                                                                                            cGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATA‑TTCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCAc                                          >  8:1155726/1‑151 (MQ=255)
                                                                                                               tGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCCGC‑GGCCCCCGCATA‑TGCCCCACATCCCGCCGCCAGCCGGCCATATTCCACGGCGCGTTCAGCCAGTGGGGGCCAATACTGTCGTGCCCCGGGTAAATTTCATTCACCac                                       >  1:687728/1‑150 (MQ=255)
                                                                                                                 aTCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATA‑TGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGAc                                     <  2:350088/151‑1 (MQ=255)
                                                                                                                 aTCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATA‑TGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGAc                                     <  5:633387/151‑1 (MQ=255)
                                                                                                                 aTCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATA‑TGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATAATGTCTTCCCCGCGATAAATTTAATTAACCAGAc                                     >  5:736916/1‑151 (MQ=255)
                                                                                                                 aTCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑GCCCCCAGCATA‑TGGCCCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCGTCCCCGCGATAAATTTAATTCACCAGAc                                     >  6:161424/1‑151 (MQ=255)
                                                                                                                 aTCCCGGCAACGCGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATA‑TGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGAc                                     <  8:1336860/151‑1 (MQ=255)
                                                                                                                  tCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATA‑TGCACCACATCCAGCCGCCAGCCGTCCATATTACACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACt                                    >  2:1129638/1‑151 (MQ=255)
                                                                                                                   cccGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATA‑TGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACt                                    <  8:1072703/150‑1 (MQ=255)
                                                                                                                   cccGGCAACGTGCTGCATATTATTGCGCGCCCCACCCCCC‑TCCCCCAGCATA‑TGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGAAAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACtt                                   >  4:1279205/1‑151 (MQ=255)
                                                                                                                    ccGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATA‑TGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACttt                                  <  2:1150444/151‑1 (MQ=255)
                                                                                                                     cGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑GCCCCCAGCATA‑TGCACCACATCCAGCCCCCACCCGTCCATATTCCACCGCGCTTTCAGCCAGTGGCGGAAAATAATGTCTTCCCCGCGATAAATTTCATTCACCAGACgtt                                  >  5:1137896/1‑150 (MQ=255)
                                                                                                                       gCAATGTGCAGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATA‑TGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCg                               <  2:55215/151‑1 (MQ=255)
                                                                                                                       gCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATA‑TGCACCCCAACCCGCCGCCCGCCGGCCATATTACACGGCGCTTTAAGCCACTGGCGGACAATACTGTCTTCCCCGCGATAAATTTGAATGACCCGACGTTccc                               >  4:167350/1‑150 (MQ=255)
                                                                                                                        cAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATA‑TGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGa                              <  8:706763/151‑1 (MQ=255)
                                                                                                                        cAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATA‑TGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGa                              <  8:220068/151‑1 (MQ=255)
                                                                                                                        cAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCACCATA‑TGCACCACAACCAGCCCCCACCCGTCCATATTAAACGGCCCGTTAAGCCAGTGGCGGACAAAACAGTCATCCCCGCGATAAATTTAAATCACCAGACGTTACg                               >  6:128579/1‑150 (MQ=255)
                                                                                                                           cGTGCTGCATATTATTGCGCGCCCCCCCCCCC‑GCCCCCCGCAAA‑TGCCCCACAACCAGCCGCCAGCCCGGCCTATTACCCGGCGCGTTCCGCCCGTGGCGGGCAATACTGTGTTGCCCGCGATAAATTTGATTGACCAGAGATTCCGACTg                           >  6:456659/1‑151 (MQ=255)
                                                                                                                              gctgcATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATA‑TGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTCCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATa                        <  3:82049/151‑1 (MQ=255)
                                                                                                                               ctgcATATTATTGCGCGCCCCCCCCGCC‑TCCCCCCGCATA‑TGCACCACATACACCCGCCCGCCGTCCAAATTACACCGCCCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCCCGATAAAATTAATTCACCATACATTACCACTGATa                        >  4:920141/1‑150 (MQ=255)
                                                                                                                               ctgcATATTATTGCGCGCCCCCCCCCCC‑GCCCCCAGCCTA‑TGCCCCACAACCCCCCCCCCCCCCGCCAAATTACACCGCCCTTTCACCCACTGGCGGCCAAAACTGTCTTGCCCGCGCTAAATTTCATTCACCACCCtttaccccagaaa                        >  5:423378/1‑140 (MQ=255)
                                                                                                                                  cATATTATTGCGCGCCCCCCCCGCC‑GCCCCCAGCATA‑TGCACCACATCCAGCCCCCAGCCGGGCCTATTCCACGGCGCGTTGAGCCAGTGGGGGACAATACTGTCTTGCCCGCGATAAATTTCATTAACCCGACTTTACGCCTGATAATcc                    >  3:11276/1‑151 (MQ=255)
                                                                                                                                   atatTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATA‑TCCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCa                   <  5:987494/151‑1 (MQ=255)
                                                                                                                                     attattGCGCGCCCCACCCGCC‑TCCCCCAGCATA‑TGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAg                  <  1:35526/150‑1 (MQ=255)
                                                                                                                                        attGCGCGCCCCACCCGCC‑TCCCCCAGCATA‑TGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCtt               <  3:927949/150‑1 (MQ=255)
                                                                                                                                         ttGCGCGCCCCACCCGCC‑TCCCCCAGCATA‑TGCGCCACATCCAGCCGCCAGCCGTCCCTATTACACCGCGCATTAAGCCAGTGGCGGACAATACTGTCTTCCCCCCGATAAATTTCATTAAACACCCGTTCCGACTTATAATCCAGCTTCg             >  1:943977/1‑151 (MQ=255)
                                                                                                                                             gcgcCCCACCCGCC‑TCCCCCAGCATA‑TGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTCGGCaa         <  4:439944/151‑1 (MQ=255)
                                                                                                                                               gcCCCACCCGCC‑TCCCCCAGCATA‑TGCACCACATCCAGCCGCCAGCCGTCGATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTCGGCAAGc       <  2:793356/151‑1 (MQ=255)
                                                                                                                                                 cccACCCGCC‑TCCCCCAGCATA‑TGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTCGGCAAGCTg     <  1:802242/151‑1 (MQ=255)
                                                                                                                                                   cACCCGCC‑TCCCCCAGCATA‑TGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTCGGCAAGCTGGc   >  3:261766/1‑151 (MQ=255)
                                                                                                                                                   cACCCGCC‑TCCCCCAGCATA‑TGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTCGGCAAGCTGGc   >  7:1351914/1‑151 (MQ=255)
                                                                                                                                                   cACCCGCC‑TCCCCCAGCATA‑TGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTCGGCAAGCTGGc   >  8:919564/1‑151 (MQ=255)
                                                                                                                                                    aCCCGCC‑TCCCCCAGCATA‑TGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTCGGCAAGCTGGc   >  2:942194/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                        
CACGATAGTTCATGGCGGCATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCC‑TCCCCCAGCATA‑TGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTCGGCAAGCTGGCA  >  DH1/3447255‑3447553

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑