Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH14,122,1240CA32.0% 284.9 / 19.5 197intergenic (‑15/+22)ECDH1_RS20430/ECDH1_RS20435tRNA‑Gly/tRNA‑Gly
Reads supporting (aligned to +/- strand):  ref base C (86/31);  new base A (54/1);  total (164/33)
Fisher's exact test for biased strand distribution p-value = 3.34e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 199 total aligned reads displayed.

CTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGCGTCGTTACGGGA  >  DH1/4121977‑4122273
                                                                                                                                                   |                                                                                                                                                     
ataacaacgtaGCTATTCCCCCTGGGGTGGTGTGTCCATTATATCACTTTAAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCttt                                                                                                                                                    <  8:255883/141‑1 (MQ=17)
   ccAACTGAGCTATTCCCTCTGGGGTGGTCTGTGGCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTgca                                                                                                                                                 <  1:245339/151‑1 (MQ=255)
   acaactatgattatCCCGCTGGGTCGGTCTGGGGCTTTGATCAATTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTgca                                                                                                                                                 <  5:129475/138‑1 (MQ=14)
    cAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCGTTACCGCACTTTGAAATTTTGGAGCGGGAAACGAGACTCGCACTCGCGCCCCCGACCTTGGCAATGTCGTGCTCGACAAACTGAGCTATTCCCGCTTGGGACGTCTGTGATGTgc                                                                                                                                                  >  7:24280/1‑149 (MQ=14)
           gCTATACGCGGTTGGTGGGTCTGTCCATTAGAGCAATTTAAAAATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTCTGCAGCACtttt                                                                                                                                         <  1:552410/151‑1 (MQ=14)
            ataaaccctggtGGGTGCTCTGTCGCTTAGATCTATTTAAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTc                                                                                                                                        <  5:750211/140‑1 (MQ=14)
             tATTCCCGCTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGAAGAACTTTTc                                                                                                                                        >  4:1225457/1‑150 (MQ=255)
               tacccGCTGGGGGGGTCTGGGCATTAAAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCaa                                                                                                                                      <  7:307183/148‑1 (MQ=17)
                 cgcgcgTGGGGGGTCTGTGACTTACATCAATATAAAAATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAAtt                                                                                                                                   <  1:666071/149‑1 (MQ=14)
                  ccccgggggCGGTGTGTCCCTTACATCCCTTGCAAAATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAAttt                                                                                                                                  <  1:382452/145‑1 (MQ=14)
                      ttGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGCGTCGTCAGTTCGATGCAGCACTTTTCAAAtttttg                                                                                                                               >  5:547430/1‑148 (MQ=17)
                      ttGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTAGCGAACCCGAACTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGCGTCGTATGTTGATTGTAGAAATTTTAAAAttattt                                                                                                                               >  2:41577/1‑146 (MQ=14)
                      gggggTGGTGTGGCCATTACATCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGa                                                                                                                              <  8:858974/149‑1 (MQ=17)
                       tttttgttatgtgTCTTACAGTACTTTAATATTTTGGTTTGTGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTgg                                                                                                                               <  2:1340302/140‑1 (MQ=14)
                       tGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGTAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTAGTTCGTCAGTTATATTGAGCAATTTTCAAAtttttgcg                                                                                                                             >  6:593482/1‑147 (MQ=14)
                        ggggggTCTGGGAATTACATCACTTTAAAATTTTGGAGCGGGAAACGAGACACGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGc                                                                                                                            <  5:337375/151‑1 (MQ=17)
                            ggtCTGGCCCTTAAATCACTTTCAAAATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAATGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGa                                                                                                                        <  5:621721/151‑1 (MQ=14)
                                tgtgCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGATTTGCAGCACTTTTCAAATTTTGGAGAGGGAAACg                                                                                                                    >  6:1163144/1‑151 (MQ=255)
                                gtggttttttatttttttAAAATTTTGGAGTGGTAAACGAGCCTGTTTCTAGCTACCCCTACCTTGGCAAGGTCGTGCTCTAACAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTTCATCACTTTTCAAATTTTGGAGCTGGAAACg                                                                                                                    <  6:1292629/136‑1 (MQ=9)
                                 gtgatttaagttggtttCAAATTTGGAGCCGCAAACGAGGCTGGTCATCCCGACCCCGACCTTGGCAAGGGAGTGCTCTAAAAACTGAGCTATTACCGCTTGGGTGGGCTGGGATTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACga                                                                                                                   <  5:1215439/136‑1 (MQ=9)
                                 gtgCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTTCTTTGCAGGACTTTTCAAATTTTGGAGGGGGAACCgc                                                                                                                   >  5:215233/1‑150 (MQ=255)
                                 gtgCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCATGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCAGACTTCTTTTATCTGAAGCAATTTTCAAATTTTTGAGAAGTAAACg                                                                                                                    >  4:889288/1‑150 (MQ=11)
                                 gtgCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGAGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGTTTTGCAGCACTTTTCAAATTTTGGAGCGGGAACCgg                                                                                                                   >  5:1319390/1‑150 (MQ=255)
                                   gCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGATTTTAAGAACTTTTCAAATTTTTGAGAGGTAAACgatc                                                                                                                 >  8:850743/1‑149 (MQ=17)
                                    cctgaccccACGTTCACATTTCCGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCAATTTTCAAATTTTGGAGCGGGAAACGAGAc                                                                                                                <  5:1031478/143‑1 (MQ=14)
                                      ttACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTACGTGGTATGTGATATGAAGTAATTTTCAAATTTTTGAGAGGGAACGGAGAGTc                                                                                                              >  2:266551/1‑151 (MQ=17)
                                       tACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGGTTTGGAGCACTTTTCAAATTTTGGAGGGGGAAACGGGGCTCg                                                                                                             >  7:792645/1‑151 (MQ=255)
                                       tACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGATTTGGAGAACTTTTCAAATTTTTGAGACGGAAACGAGACTcc                                                                                                             >  8:1269169/1‑150 (MQ=17)
                                        aCAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTCGTTGGTCTGTTCTGTTCAGGACTTTTCACATTTAGGAGAGGGAAACgcgagggg                                                                                                             >  6:646669/1‑146 (MQ=17)
                                         cAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGGTGTGGAGCACTTTTCAAATTTTGGAGAGGGaaaggcgccggga                                                                                                            >  7:927786/1‑140 (MQ=17)
                                         cAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTAGTTGTTATTTTATATTAAGTAATTTTCAAATTTATGAGACGGAAACgatagaaga                                                                                                            >  2:517207/1‑143 (MQ=11)
                                           gCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTTCAGCACTTTTCACATTTTGGAGCGGGTAACgcgccgcgatc                                                                                                          >  7:225636/1‑140 (MQ=14)
                                            cACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGATTTGCAGCAATTTTCTAATTTTGGAGAGGGTAACGAGAGGCGaagtg                                                                                                        >  2:311543/1‑148 (MQ=14)
                                             aCTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGGGTGGTCTGTGGTGTGCAGCACTTTTCAAATTTTGGAGCGGGAACCGAGCCTCGAACTcg                                                                                                       >  3:914310/1‑151 (MQ=255)
                                                     aTTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTCGTCAGTGATATGAAGAACTTTTCAAATTTTGGAGAGGGAAACGAGACTCGAACTCGCGAcaccc                                                                                               >  8:570532/1‑150 (MQ=14)
                                                               gggAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTAGGTGTTATGTTATGTTGAGGACTTTTCTAATTTTGGAGAGGTAAACGAGCGGCGAAAAAGCGAcccctccataccga                                                                                      >  2:1368891/1‑140 (MQ=2)
                                                                ggAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTTTGCTGTGGAGCACTTTTCAAATTTAGGAGCGGGAAACGAGACGGGAACTCGCGACCCCGACCtcgcga                                                                                      >  3:452728/1‑144 (MQ=14)
                                                                    aCGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTATTTTATTTGGAGAACTTTTCAAATTTTTGAGCGGGAACCGAGCCTCCAACTAGCGACACCTCACTTGGCAAGCTcc                                                                                >  2:1446611/1‑150 (MQ=14)
                                                                      gagaCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGGGTGGTCTGTGATTTGCCGCACTTTTCAAATTTTGGCGCGGGAAACGCGACTCGATCTCGCGACCCCGACCTGGGCAAGGTCGTg                                                                              >  5:117747/1‑151 (MQ=14)
                                                                       agaCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACACCGACCTTGGCAAGGTCGTGc                                                                             >  3:173210/1‑151 (MQ=38)
                                                                       agaCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGAGTTCTGTGCTTTGAAGCAATTTTCAAATTTTTGAGAGGGAAACGCGACTCGAACTAGCGACCACGACCTTCGCAAGCTCGtcc                                                                             >  4:364931/1‑149 (MQ=14)
                                                                             gAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTCCTCTAcc                                                                       >  3:730285/1‑151 (MQ=255)
                                                                                 tCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGCCTCGAACTCGCGACCCCGCCCTTGGGAAGGTCGTGCTCTGCCACCt                                                                   >  1:283532/1‑151 (MQ=255)
                                                                                  cgcgACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGATTTGCAGCACTTTTCAAATTTTGGCGCGGGAAACGAGCCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTg                                                                  >  4:716720/1‑151 (MQ=255)
                                                                                   gcgACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTGTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTGGGCAAGGTCGTGCTCTACCAACTg                                                                  >  6:406923/1‑150 (MQ=255)
                                                                                    cgACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAACCGAGCCTCGAACTCGCGACCCCGCCCTTGGCAAGCTCGTGCTCTACCAACTg                                                                  >  2:723360/1‑149 (MQ=255)
                                                                                     gACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGCCTCGAACTCGCGACCCCGACCTGGGCAAGGTCGTGCTCTACCAACTGAgg                                                               >  3:1325949/1‑150 (MQ=255)
                                                                                      aCCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCt                                                              >  5:1239416/1‑151 (MQ=38)
                                                                                       ccccGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGGACTTTTCGAATTTTGGAGAGGGAACCGCGGCTCGATCTAGCGACCACGAGCTTGGCAAGGTCGTGCTCTACAAACTGAGCtt                                                             >  5:931561/1‑150 (MQ=14)
                                                                                       ccccGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTACGAGGTATGTGATATTAAGAAATTTTCAAATTTTGGAGCGCGAAACGAGACTCGAACTAGCGACCCCGAGCTACGCAAGGACGTCCACTAACAACTGAGCt                                                              >  6:691504/1‑150 (MQ=11)
                                                                                        cccGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGGTTTGCAGCACTTTTCCAATTTTGGAGCGGGAAACGAGCCTCGAACTAGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTAt                                                            >  5:692373/1‑151 (MQ=255)
                                                                                        cccGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGAGGGAAAGGAGAGTCGATCTCGCGACCCCGAGCTTGGCAAGGTCGTGCTCTACCAACTGAGCTAt                                                            >  3:982079/1‑151 (MQ=255)
                                                                                           gACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGATTTGCAGCAATTTTCAAATTTAGGAGAGGGAAACGAGCGTCGAACTATCGACAACGACCTCCGCAAGGTCGAGCTCTACAAACTGAGCTTTTcc                                                         >  5:523984/1‑151 (MQ=14)
                                                                                                tGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGGGTGGTATGTGATGTGGAGGACTTTTCAAATTTTGGAGAGGGAACCGCGACGCGAAATCGCGACCCCGCGCTTGGGAAGGTCGTCCGCAACCAACTGAGCTATTccctctt                                                    >  5:113513/1‑147 (MQ=11)
                                                                                                  gCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCACGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGtac                                                  >  3:1027682/1‑151 (MQ=255)
                                                                                                   cAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGGTTTGCAGCACTTTTCAAATTTTGGAGAGGGAAACGAGACTCGAACTCGCGACCCCGACCTTCCCAAGGTCGTGCTCTACAAACTGAGCTATTCCCGAGtac                                                  >  2:1241613/1‑150 (MQ=14)
                                                                                                     aGGTCGTGGTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACACCGACATTGGCAAGGTCGTGCCCTACAAACTGAGCTATTCCAGCGtactac                                               >  3:1264564/1‑151 (MQ=14)
                                                                                                       gTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACtt                                             >  7:1137052/1‑151 (MQ=255)
                                                                                                          gTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTTCTGTGGAGGACTTTTCAAATTTAGGCGCGGGAAACGAGACTCGAACTCGCGACCCCGCCCTTGCCAAGGTCCTGCACTACCAACTGAGGTATTCCCTGGTACGACTTCAt                                          >  6:721664/1‑151 (MQ=14)
                                                                                                          gTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGATTTGAAGAACTTTTCAAATTTTGGAGAGGGAAACGAGACTCGAAATAACGACACCGAAATAGGAAAGGTCGTGCTCTACAAACTGAGCTATACACGCTTACtaattaat                                          >  6:505452/1‑151 (MQ=14)
                                                                                                           tGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGCGCCTCGAACTCGCGACCCAGCCCTTGGCAAGGTCGTGCGCTACAAACTGAGCTATTCCCGCGTAGTACTTAAtt                                         >  3:1436014/1‑151 (MQ=14)
                                                                                                           tGCTCTACCAACTGAGCTATTCCCGCGTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGCGCGGGAAACGAGACTCGAACTCGCGACCCCGACCGTGCAAAGGTCGTGCGCTAACAACTGGGCTATTCCCGCGTACTACTTAAtt                                         >  2:161999/1‑151 (MQ=17)
                                                                                                            gCTCTACCAACTGAGCTATTCCCGCTTGCGTGGTATGTGATATGCAGGACTTTTCAAATTTTTGAGCGGGAAACGAGACACGAACTCGCGACCACGACGTAGGGAAGGTCGTGCACTACCAACTTAGCTATTCCAGATTACTACTTAAttt                                        >  6:99413/1‑151 (MQ=14)
                                                                                                              tctACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTAGGAGCGGGAAACGAGACTCGAACTAGCGACACCGACCTAGGCAAGGTCGTGCTCTACAAACTGAGCTATTCCCGCTTAGTACTTAATTTTg                                      >  2:859635/1‑151 (MQ=14)
                                                                                                               ctACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGc                                     >  7:180627/1‑151 (MQ=255)
                                                                                                                 aCCAACTGAGCTATTCCCGCTTGGTTCGTAAGTTATTTGCAGCAATTTTCAAATTTATGAGAGGTAAACGAGACACGATATATCGACAAAGAAGTTGGAAAGGTCGTCCACTAACAACTGATATATTCCCGAGTACTACTTAATTTTGAtt                                   >  8:537199/1‑151 (MQ=12)
                                                                                                                 aCCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCtt                                   >  7:501826/1‑151 (MQ=255)
                                                                                                                 aCCAACTGAGCTATTCCCGCTTGGGAGGTCTGTGCTTTGGAGAACTTTTCAAATTTTGGAGAGGGAAACCAGACACGAACTAGCGACCCCGAGCTTCCGAAGGACGTGCACAAAAAATTGAGCTATTCCCGCGTACTTCATAAttttaatt                                   >  2:690187/1‑147 (MQ=9)
                                                                                                                     aCTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGt                               >  8:114404/1‑151 (MQ=255)
                                                                                                                     aCTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGt                               >  1:1112020/1‑151 (MQ=255)
                                                                                                                      cTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGt                               >  3:296075/1‑150 (MQ=255)
                                                                                                                       tGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTa                              >  5:234751/1‑150 (MQ=255)
                                                                                                                         aGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAAtt                           >  4:115103/1‑151 (MQ=255)
                                                                                                                         aGCTATTCCCGCTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTTGAGCGGGAAACGATACTCGAACTAGCGACCCCGACCTTGGCAAGGTCGTGCTAAAACAACTGAGCTATTCCCGCGTGCGACGTAAgttagttggagcaagtg                          >  6:821241/1‑137 (MQ=11)
                                                                                                                           cTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAAtttt                         >  3:1404519/1‑151 (MQ=255)
                                                                                                                           cTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAAtttt                         >  6:1018985/1‑151 (MQ=255)
                                                                                                                            tATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTc                        >  8:1080900/1‑151 (MQ=255)
                                                                                                                             aTTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCg                       >  3:100872/1‑151 (MQ=255)
                                                                                                                              ttCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGc                      >  5:359239/1‑151 (MQ=255)
                                                                                                                               tCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGc                      >  1:1255803/1‑150 (MQ=255)
                                                                                                                                 ccGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGCCCTATTCCCGCGAACTACTTAATTTAGCTTACGTAATTTTCGCAtt                   >  4:594138/1‑151 (MQ=255)
                                                                                                                                 ccGCTTGGGTGGTCTGGGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCAtt                   <  5:633646/151‑1 (MQ=255)
                                                                                                                                   gCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGCCCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCAttt                  >  6:1223696/1‑150 (MQ=255)
                                                                                                                                   gCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCAttt                  >  8:147768/1‑150 (MQ=255)
                                                                                                                                   gCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTc                 >  4:15533/1‑151 (MQ=255)
                                                                                                                                    cTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCt                >  4:810816/1‑151 (MQ=255)
                                                                                                                                    cTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCt                >  1:993046/1‑151 (MQ=255)
                                                                                                                                       gggTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGcg             >  4:255190/1‑151 (MQ=255)
                                                                                                                                       gggTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGcg             >  8:1161308/1‑151 (MQ=255)
                                                                                                                                        ggtggtCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGcgt            >  3:505042/1‑151 (MQ=255)
                                                                                                                                         gtggtCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAAGGAGCCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTGTGcgtc           >  5:1441168/1‑151 (MQ=255)
                                                                                                                                            gtCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGCGTCGtt        >  7:105536/1‑151 (MQ=255)
                                                                                                                                              cTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGCGTCGTTAc      >  3:868516/1‑151 (MQ=255)
                                                                                                                                               tgtgCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGCGTCGTTACg     >  1:885118/1‑151 (MQ=255)
                                                                                                                                               tgtgCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAAGTTTGCTTTCGTAATTTTCGCATTTCTGCGTCGTTACg     >  5:813195/1‑151 (MQ=255)
                                                                                                                                               tgtgCTTAGCCGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAACGTCGTGCTCTACCCACTGAGCGCTTCCCGCCTACTACTTAATTTTGCATTCGTAATTTACGCATATCTGCGTCGTAACg     >  6:175404/1‑151 (MQ=18)
                                                                                                                                                 tgCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGCGTCGTTACggg   >  7:430820/1‑151 (MQ=255)
                                                                                                                                                 tgCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGATTTCGTAATTTTCGCATTTCTGCGTCGTTACggg   >  7:1378273/1‑151 (MQ=255)
                                                                                                                                                   cTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGCGTCGTTACggg   >  1:1057664/1‑149 (MQ=255)
                                                                                                                                                   cTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGCGTCGTTACggg   >  1:1378496/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGCGTCGTTACGGGA  >  DH1/4121977‑4122273

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: