Predicted mutation
evidence seq id position mutation annotation gene description
RA DH1 205,979 T→C D189D (GAT→GAC)  yhjG → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1205,9790TC99.2% 1521.0 / NA 394D189D (GAT→GAC) yhjGhypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (181/209);  minor base G (1/2);  total (183/211)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 398 total aligned reads displayed.

GGACGTTTAATCTTGCCAACGATGATAACAAAGACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGATCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGAACCGCTCACGGGTACGGGAAAAATAGGCGGT  >  DH1/205831‑206126
                                                                                                                                                    |                                                                                                                                                   
        aaTCTTGCCAACGATGATAACAAAGACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGc                                                                                                                                            <  4:821846/150‑1 (MQ=255)
        aaTCTTGCCAACGATGATAACAAAGACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAACGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGc                                                                                                                                            >  1:459464/1‑150 (MQ=255)
         aTCTTGCCAACGATGATAACAAAGACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCa                                                                                                                                           >  7:1101153/1‑150 (MQ=255)
           cTTGCCAACGATGATAACAAAGACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATAGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACACCTTAGGCa                                                                                                                                           >  4:1092291/1‑148 (MQ=255)
             tGCCAACGATGATAACAAAGACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCaa                                                                                                                                          <  4:1441809/147‑1 (MQ=255)
               ccAACGATGATAACAAAGACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCt                                                                                                                                    <  3:928449/151‑1 (MQ=255)
                cAACGATGATAACAAAGACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTg                                                                                                                                   >  3:1028077/1‑151 (MQ=255)
                cAACGATGATAACAAAGACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTg                                                                                                                                   >  8:720423/1‑151 (MQ=255)
                  aCGATGATAACAAAGACTCGAATGCAAAGCCGTCGGCATGGTCTTTTCGCCTGGATAATATTCTTTTCGATCAAGGTCGGATCGACATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTAGTTGACCCCTTAGGCAAGCCGCTGcc                                                                                                                                 >  5:1334368/1‑151 (MQ=255)
                      tgatAACAAAGACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTc                                                                                                                             >  7:626547/1‑151 (MQ=255)
                       gatAACAAAGACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCa                                                                                                                            <  2:691197/151‑1 (MQ=255)
                       gatAACAAAGACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCa                                                                                                                            <  8:883164/151‑1 (MQ=255)
                        atAACAAAGACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTc                                                                                                                             >  7:956988/1‑149 (MQ=255)
                         tAACAAAGACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGc                                                                                                                          >  1:794477/1‑151 (MQ=255)
                          aaCAAAGACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCg                                                                                                                         <  5:721003/151‑1 (MQ=255)
                          aaCAAAGACGCGAATGCAAAGCCGTCGGCATGCTCGTTTCGGCTGGATAATATTCTATTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTAGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCg                                                                                                                         >  4:386966/1‑151 (MQ=255)
                           aCAAAGACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATC‑AGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGaa                                                                                                                       <  1:1397743/151‑1 (MQ=255)
                               aGACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGtt                                                                                                                    >  1:672826/1‑151 (MQ=255)
                                gACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGtt                                                                                                                    >  4:980378/1‑150 (MQ=255)
                                  cgcgAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACt                                                                                                                 <  5:247693/151‑1 (MQ=255)
                                  cgcgAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACt                                                                                                                 <  3:206679/151‑1 (MQ=255)
                                  cgcgAATGCAAAACCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACt                                                                                                                 <  2:352712/151‑1 (MQ=255)
                                   gcgAATGCAAAGCCGTCTGCATGGTCTTTTCGTCTGGATAATATTCTTTTCGATCAAGGGCGGATCTCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTg                                                                                                                <  3:300647/151‑1 (MQ=255)
                                   gcgAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTCTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTg                                                                                                                >  2:1452529/1‑151 (MQ=255)
                                       aTGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATc                                                                                                            <  8:1425752/151‑1 (MQ=255)
                                            aaGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAg                                                                                                       <  1:1098629/151‑1 (MQ=255)
                                               ccGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTa                                                                                                    <  1:1176368/151‑1 (MQ=255)
                                                   cGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGc                                                                                                <  3:391790/151‑1 (MQ=255)
                                                     gCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCgg                                                                                              <  4:136378/151‑1 (MQ=255)
                                                      cATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGa                                                                                             <  8:210217/151‑1 (MQ=255)
                                                         ggTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGCCAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATa                                                                                           >  2:788552/1‑150 (MQ=255)
                                                          gTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATaa                                                                                          >  3:991471/1‑150 (MQ=255)
                                                            cGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATaa                                                                                          >  6:1453666/1‑148 (MQ=255)
                                                              tttCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGaa                                                                                      >  4:640766/1‑150 (MQ=255)
                                                                 cGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAgg                                                                                  >  6:370303/1‑151 (MQ=255)
                                                                 cGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCCGATCTGGAGATTTTTGTTGACCCCTTAGGCAGGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAg                                                                                   <  4:547545/150‑1 (MQ=255)
                                                                   gCTTGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTg                                                                                <  2:495652/151‑1 (MQ=255)
                                                                   gCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTg                                                                                <  2:1066556/151‑1 (MQ=255)
                                                                      ggATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCGCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTgggg                                                                             >  1:381337/1‑150 (MQ=255)
                                                                      ggATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGc                                                                             <  7:1292804/151‑1 (MQ=255)
                                                                      ggATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGc                                                                             <  7:1252487/151‑1 (MQ=255)
                                                                        ataataTTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGa                                                                           <  7:1461492/151‑1 (MQ=255)
                                                                          aataTTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGAtt                                                                         >  3:1376544/1‑151 (MQ=255)
                                                                          aataTTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGAtt                                                                         <  5:629880/151‑1 (MQ=255)
                                                                           atatTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGAtt                                                                         >  7:185614/1‑150 (MQ=255)
                                                                           atatTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGAtt                                                                         >  3:707411/1‑150 (MQ=255)
                                                                           atatTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGACCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGAtt                                                                         >  8:500723/1‑150 (MQ=255)
                                                                             atTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGCAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACg                                                                      <  1:296411/151‑1 (MQ=255)
                                                                             atTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTAc                                                                       <  4:69864/150‑1 (MQ=255)
                                                                                  tttCGTTCAATGGGTGATCGCCATTGATGACAATGTAAGCTATTCGGATCTGGAGATTTTTGTTGACCTCTTAGGCAAGCCGCTGCGGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATATTGATAAGGTGGGCGATTACGtttt                                                                  <  6:679188/150‑1 (MQ=255)
                                                                                  tttCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGGAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGtttt                                                                  <  5:674274/150‑1 (MQ=255)
                                                                                  tttCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGtt                                                                    <  3:1314206/148‑1 (MQ=255)
                                                                                  tttCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGtt                                                                    <  2:1395581/148‑1 (MQ=255)
                                                                                  tttCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGAGCTGGAGATTTTTGTTGCCCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGtttt                                                                  <  6:1064825/150‑1 (MQ=255)
                                                                                     cGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTgg                                                               <  2:428955/150‑1 (MQ=255)
                                                                                      gATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGcc                                                             <  4:218558/151‑1 (MQ=255)
                                                                                       aTCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCt                                                            <  1:1007443/151‑1 (MQ=255)
                                                                                       aTCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCt                                                            >  7:459023/1‑151 (MQ=255)
                                                                                       aTCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCt                                                            >  6:556913/1‑151 (MQ=255)
                                                                                         cAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTg                                                           >  8:1292889/1‑150 (MQ=255)
                                                                                          aaGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGa                                                          >  6:759761/1‑150 (MQ=255)
                                                                                           aGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGa                                                          >  8:309464/1‑149 (MQ=255)
                                                                                           aGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGa                                                          >  6:1302281/1‑149 (MQ=255)
                                                                                           aGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAg                                                        <  2:1164601/151‑1 (MQ=255)
                                                                                                  aTCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAgg                                                 <  1:176522/151‑1 (MQ=255)
                                                                                                    cGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAggg                                                <  3:149495/150‑1 (MQ=255)
                                                                                                        aTTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGAAGat                                           <  5:924458/151‑1 (MQ=255)
                                                                                                          tgatgaCAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGatat                                         >  6:1208974/1‑151 (MQ=255)
                                                                                                           gatgaCAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGatat                                         <  5:1119501/150‑1 (MQ=255)
                                                                                                            atgaCAAAGTAAGCATAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATaa                                       <  8:1173200/151‑1 (MQ=255)
                                                                                                            atgaCAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGAtata                                        >  7:749618/1‑150 (MQ=255)
                                                                                                             tgaCAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAAc                                      >  1:1268686/1‑151 (MQ=255)
                                                                                                              gaCAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAAc                                      <  6:431007/150‑1 (MQ=255)
                                                                                                               aCAAAGTAAGCAAAGCGGTTCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCGGTTCAGCGAAGTTACTGGATCGAAAGTTAAAGCGGATAAAGATAAGTTGGGCGATTACGTTTTTGGCGTGAAGGCGCAGGGACGATATAACgg                                    <  4:319225/151‑1 (MQ=255)
                                                                                                               aCAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACg                                     <  5:1375570/150‑1 (MQ=255)
                                                                                                                 aaaGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTg                                  >  3:1129869/1‑151 (MQ=255)
                                                                                                                 aaaGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTg                                  >  3:757347/1‑151 (MQ=255)
                                                                                                                 aaaGTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCCCAGGGACGATATAACGGTg                                  >  1:1036394/1‑151 (MQ=255)
                                                                                                                    gTAAGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGAAc                               >  3:62281/1‑151 (MQ=255)
                                                                                                                      aaGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGAAcc                              <  7:1147625/150‑1 (MQ=255)
                                                                                                                      aaGCAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGAACCg                             <  8:957364/151‑1 (MQ=255)
                                                                                                                        ccAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGAACCGCt                           <  7:323711/150‑1 (MQ=255)
                                                                                                                         cAAAGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGAACCGCTc                          >  5:1292204/1‑151 (MQ=255)
                                                                                                                          aaaGCGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGAACCGCTc                          >  6:794917/1‑150 (MQ=255)
                                                                                                                              cGGATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGAACCGCTCACggg                     >  8:112268/1‑151 (MQ=255)
                                                                                                                               ggATCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGAACCGCTCACGGGt                    <  1:909625/151‑1 (MQ=255)
                                                                                                                                 aTCTGGAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCGTGAAGGCGCAGGGACGATATAACGGTGAACCGCTCACGGGTAc                  >  2:228592/1‑151 (MQ=255)
                                                                                                                                     ggAGATTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGAACCGCTCACGGGTACggg               >  7:1014160/1‑150 (MQ=255)
                                                                                                                                      gagaTTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGAACCGCTCACGGGTACGGGa              >  6:176969/1‑150 (MQ=255)
                                                                                                                                      gagaTTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGAACCGCTCACGGGTACGGGa              >  4:728977/1‑150 (MQ=255)
                                                                                                                                       agaTTTTTGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGAACCGCTCACGGGTACGGGaa             >  6:143271/1‑150 (MQ=255)
                                                                                                                                          tttttGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCGTGAAGGCGCAGGGACGATATAACGGTGAACCGCTCACGGGTACGGGAAAAAt         >  5:46402/1‑151 (MQ=255)
                                                                                                                                           ttttGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGAACCGCTCACGGGTACGGGAAAAAt         >  7:1318675/1‑150 (MQ=255)
                                                                                                                                            tttGTTGACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGAACCGCTCACGGGTACGGGAAAAAt         >  6:923611/1‑149 (MQ=255)
                                                                                                                                             ttgttgACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGAACCGCTCACGGGTACGGGAAAAATAgg      >  3:1180961/1‑151 (MQ=255)
                                                                                                                                             ttgttgACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGAACCGCTCACGGGTACGGGAAAAATAg       <  2:627769/150‑1 (MQ=255)
                                                                                                                                               gttgACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGAACCGCTCACGGGTACGGGAAAAATAGGc     <  4:1030958/150‑1 (MQ=255)
                                                                                                                                               gttgACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGAACCGCTCACGGGTACGGGAAAAATAGGCg    >  8:392953/1‑151 (MQ=255)
                                                                                                                                               gttgACCCCTTAGGCAAGCCGCTGCCGTTCAGAGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGGGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGAACCGCTCACGGGTACGGGAAAAATAGGc     <  7:1220239/150‑1 (MQ=255)
                                                                                                                                                 tgACCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGAACCGCTCACGGGTACGGGAAAAATAGGCGGt  >  5:367567/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GGACGTTTAATCTTGCCAACGATGATAACAAAGACGCGAATGCAAAGCCGTCGGCATGGTCGTTTCGGCTGGATAATATTCTTTTCGATCAAGGGCGGATCGCCATTGATGACAAAGTAAGCAAAGCGGATCTGGAGATTTTTGTTGATCCCTTAGGCAAGCCGCTGCCGTTCAGCGAAGTTACTGGATCGAAAGGTAAAGCGGATAAAGAAAAGGTGGGCGATTACGTTTTTGGCCTGAAGGCGCAGGGACGATATAACGGTGAACCGCTCACGGGTACGGGAAAAATAGGCGGT  >  DH1/205831‑206126

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑