Predicted mutation
evidence seq id position mutation annotation gene description
RA DH1 2,140,096 T→C E180G (GAA→GGA)  purR ← PurR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH12,140,0960TC97.8% 1157.4 / 6.3 312E180G (GAA→GGA) purRPurR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (4/3);  new base C (156/149);  total (160/152)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 312 total aligned reads displayed.

CGCGATAACCGGATTCAGGTTCAAAGTCACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTTCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTGGGATATGGCGATACTCTTCCAGCATCGCC  >  DH1/2139950‑2140242
                                                                                                                                                  |                                                                                                                                                  
cgcgATAACCGGATTCAGGTTCAAAGTCACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCcgcg                                                                                                                                                >  7:1446054/1‑151 (MQ=255)
  cgATAACCGGATTCAGGTTCAAAGTCACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGt                                                                                                                                              <  1:869014/151‑1 (MQ=255)
    aTAACCGGATTCAGGTTCAAAGTCACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTg                                                                                                                                             >  4:949066/1‑150 (MQ=255)
    aTAACCGGATTCAGGTTCAAAGTCACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTg                                                                                                                                             >  5:474084/1‑150 (MQ=255)
    aTAACCGGATTCAGGTTCAAAGTCACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGa                                                                                                                                            <  7:1278006/151‑1 (MQ=255)
      aaCCGGATTCAGGTTCAAAGTCACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGAcc                                                                                                                                          >  2:1339940/1‑151 (MQ=255)
         cGGATTCAGGTTCAAAGTCACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACcgcg                                                                                                                                       <  6:30705/151‑1 (MQ=255)
         cGGATTCAGGTTCAAAGTCACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCAATTCCGCGGTGACcgcg                                                                                                                                       <  3:1239158/151‑1 (MQ=255)
                aGGTTCAAAGTCACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATc                                                                                                                                <  5:383099/151‑1 (MQ=255)
                aGGTTCAAAGTCACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATc                                                                                                                                <  8:145811/151‑1 (MQ=255)
                  gTTCAAAGTCACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATc                                                                                                                                >  5:841778/1‑149 (MQ=255)
                  gTTCAAAGTCACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAg                                                                                                                              <  5:198057/151‑1 (MQ=255)
                     cAAAGTCACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGAt                                                                                                                            <  2:664737/150‑1 (MQ=255)
                       aaGTCACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGCCGCCGATTCCGCGGTGACCGCGTTCAATCAGATAAc                                                                                                                         >  2:223873/1‑151 (MQ=255)
                            aCCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCg                                                                                                                    >  6:662768/1‑151 (MQ=255)
                             cccTGCACAATCGAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCgg                                                                                                                   <  4:469609/151‑1 (MQ=255)
                                 gCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGcc                                                                                                                 <  3:505962/149‑1 (MQ=255)
                                    caATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTa                                                                                                            <  8:1299015/151‑1 (MQ=255)
                                    caATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCAGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTa                                                                                                            <  3:933190/151‑1 (MQ=255)
                                     aaTCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCTGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGAGTTCAATCAGATACCGCCCGGCCATGt                                                                                                             >  6:1140240/1‑149 (MQ=255)
                                     aaTCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAg                                                                                                           <  7:1018380/151‑1 (MQ=255)
                                       tCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAgcc                                                                                                         >  4:129106/1‑151 (MQ=255)
                                       accAGCGTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAgcc                                                                                                         <  3:111422/150‑1 (MQ=255)
                                        ccAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAgcc                                                                                                         <  4:324087/150‑1 (MQ=255)
                                        ccAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAgcc                                                                                                         <  8:1178019/150‑1 (MQ=255)
                                         caccTTTCCGGCACCTTGATCATCGCTTCTACCATCGCCTTCATAAAACCGGCAAGGCCGCCTGCCCCGGTGTTACGTTCCAGCGGGCCGCGGATGACCCCGATTCCGCGGTGACCGCGTGCAATCAGATAACGCCCGGCCATGTAgccgc                                                                                                       >  6:1259488/4‑151 (MQ=255)
                                            cTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCGTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGAGACCGCGTTCAATCAGATAACGCCCGGCCATCTAGCCGCCtt                                                                                                    >  8:1051950/1‑151 (MQ=255)
                                             tttCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGTCAAGGCGGCCTGCGCCGGTGTTACGTTACAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGACAGGCCATGTAGCCGCCTTc                                                                                                   >  1:388532/1‑151 (MQ=255)
                                             tttCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTc                                                                                                   >  1:939960/1‑151 (MQ=255)
                                             tttCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTc                                                                                                   >  4:1247409/1‑151 (MQ=255)
                                             tttCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTc                                                                                                   >  3:316064/1‑151 (MQ=255)
                                              ttCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTc                                                                                                   <  2:799427/150‑1 (MQ=255)
                                                ccGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGa                                                                                                 >  7:735786/1‑150 (MQ=255)
                                                  ggCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAAcg                                                                                              >  1:244601/1‑151 (MQ=255)
                                                    cACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAAcgcg                                                                                            >  2:110061/1‑151 (MQ=255)
                                                     aCCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGCCGCCGATTCCGCGGTGACCGCGTTCACTCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGt                                                                                           >  4:269905/1‑151 (MQ=255)
                                                     aCCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGt                                                                                           >  3:1250510/1‑151 (MQ=255)
                                                      ccTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGtt                                                                                          <  5:1404857/151‑1 (MQ=255)
                                                      ccTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGtt                                                                                          >  3:1199604/1‑151 (MQ=255)
                                                       cTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGtt                                                                                          >  8:737262/1‑150 (MQ=255)
                                                         tGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTa                                                                                         <  6:468679/149‑1 (MQ=255)
                                                         tGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTAt                                                                                        <  8:608719/150‑1 (MQ=255)
                                                         tGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTAt                                                                                        <  4:828592/150‑1 (MQ=255)
                                                         tGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATc                                                                                       >  5:1347286/1‑151 (MQ=255)
                                                          gATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATc                                                                                       <  3:1000622/150‑1 (MQ=255)
                                                           atcatcGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCaa                                                                                     <  5:319541/151‑1 (MQ=255)
                                                           atcatcGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCa                                                                                      >  1:495680/1‑150 (MQ=255)
                                                            tcatcGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAAt                                                                                    >  7:254023/1‑151 (MQ=255)
                                                                cGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGAcc                                                                                <  1:1094438/151‑1 (MQ=255)
                                                                   ttcttcCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGGTATCAATGACCGc                                                                              >  4:549565/1‑150 (MQ=255)
                                                                   ttcttcCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGAGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGc                                                                              >  3:1381548/1‑150 (MQ=255)
                                                                    tcttcCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCa                                                                             <  7:817249/150‑1 (MQ=255)
                                                                    tcttcCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCAt                                                                            >  5:1196242/1‑151 (MQ=255)
                                                                    tcttcCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCAt                                                                            >  6:806873/1‑151 (MQ=255)
                                                                     cttcCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATc                                                                           >  1:806258/1‑151 (MQ=255)
                                                                       tcCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCgg                                                                         >  1:1000021/1‑151 (MQ=255)
                                                                       tcCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCgg                                                                         >  1:992845/1‑151 (MQ=255)
                                                                        ccATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGt                                                                        >  4:1417636/1‑151 (MQ=255)
                                                                         cATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTg                                                                       >  5:551898/1‑151 (MQ=255)
                                                                          aTCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGa                                                                      <  6:1176769/151‑1 (MQ=255)
                                                                           tCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGa                                                                      >  2:1382822/1‑150 (MQ=255)
                                                                             gCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGt                                                                   >  8:251367/1‑151 (MQ=255)
                                                                              ccTTCATAAAACCGGCAAGGCGGCCTGCGCCGGAGTTACGTACCAGCGGGCCGGGGATGAAGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGAAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTc                                                                  >  6:350754/1‑151 (MQ=255)
                                                                               cTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTc                                                                  >  2:936967/1‑150 (MQ=255)
                                                                                 tCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCa                                                                 <  3:234350/149‑1 (MQ=255)
                                                                                  cATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCt                                                              >  6:719792/1‑151 (MQ=255)
                                                                                    tAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCttt                                                            >  2:526773/1‑151 (MQ=255)
                                                                                    aaaaaCCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGCCCGCGCATGACGCCGATTCCGCGGAGACCGCGTTCAATCAGATAACGCACGCCCATGTAGCCGCCTTCGAACGCGTAATCAATGACCGCATCGGTGAAGTCAGCttt                                                            >  7:514558/2‑151 (MQ=255)
                                                                                      aaaCCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTg                                                          <  8:900694/151‑1 (MQ=255)
                                                                                       aaCCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGc                                                         <  7:1384046/151‑1 (MQ=255)
                                                                                        aCCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCt                                                        <  7:412358/151‑1 (MQ=255)
                                                                                              aaGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCAccc                                                  >  1:337904/1‑151 (MQ=255)
                                                                                               aGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCAcccc                                                 >  8:92504/1‑151 (MQ=255)
                                                                                                  cggcCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGt                                              <  5:891691/151‑1 (MQ=255)
                                                                                                         cgcCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGac                                       >  5:821576/1‑151 (MQ=255)
                                                                                                            cGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGaccac                                    >  3:651786/1‑151 (MQ=255)
                                                                                                                gtTACGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCAt                                 <  8:418285/150‑1 (MQ=255)
                                                                                                                gtTACGTTCCAGCGGGCCGGGGAAGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCAtt                                <  7:89553/151‑1 (MQ=255)
                                                                                                                   aCGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTggg                             <  5:573978/151‑1 (MQ=255)
                                                                                                                    cGTTCCAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTGGGa                            <  7:1058714/151‑1 (MQ=255)
                                                                                                                        ccAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCGATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTGGGatat                         <  5:1284970/150‑1 (MQ=255)
                                                                                                                        ccAGCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTGGGATATg                        >  5:1096203/1‑151 (MQ=255)
                                                                                                                           gCGGGCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTGGGATATGGCg                     <  3:722755/151‑1 (MQ=255)
                                                                                                                             gggCCGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTGGGATATGGCGAt                   >  6:3920/1‑151 (MQ=255)
                                                                                                                              ggCCGTGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTGGGATATGGCGATa                  <  3:174788/151‑1 (MQ=255)
                                                                                                                                ccGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTGGGATATGGCGATAct                <  7:678198/151‑1 (MQ=255)
                                                                                                                                 cGGGGATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTGGGATATGGCGATActg               >  4:191562/1‑150 (MQ=255)
                                                                                                                                  ggggATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTGGGATATGGCGATActct              >  6:1019496/1‑151 (MQ=255)
                                                                                                                                  ggggATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTGGGATATGGCGATActct              <  1:597599/151‑1 (MQ=255)
                                                                                                                                    ggATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTGGGATATGGCGATACTCTTc            <  4:1390681/151‑1 (MQ=255)
                                                                                                                                    ggATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTGGGATATGGCGATACTCTTc            <  4:404752/151‑1 (MQ=255)
                                                                                                                                     gATGACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTGGGATATGGCGATACTCTTcc           >  1:523276/1‑151 (MQ=255)
                                                                                                                                      aTGACGCCGATTCCGCTGTGACCTCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTGGGATATTGCGATACTATTCCa          <  2:389755/151‑1 (MQ=255)
                                                                                                                                        gACGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTGGGATATGGCGATACTCTTCCAGc        <  6:551892/151‑1 (MQ=255)
                                                                                                                                         aCGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTGGGATATGGCGATACTCTTCCAGCa       <  8:219161/151‑1 (MQ=255)
                                                                                                                                          cGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTGGGATATGGCGATACTCTTCCAGCAt      <  1:686781/151‑1 (MQ=255)
                                                                                                                                          cGCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTGGGATATGGCGATACTCTTCCAGCAt      <  1:145895/151‑1 (MQ=255)
                                                                                                                                           gCCGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTGGGATATGGCGATACTCTTCCAGCATc     >  1:1172917/1‑151 (MQ=255)
                                                                                                                                            ccGATTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTGGGATATGGCGATACTCTTCCAGCATCg    <  5:1139464/151‑1 (MQ=255)
                                                                                                                                               aTTCCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTGGGATATGGCGATACTCTTCCAGCATCGcc  >  1:1393942/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CGCGATAACCGGATTCAGGTTCAAAGTCACCCTGCACAATCCAGCTTTCCGGCACCTTGATCATCGCTTCTTCCATCGCCTTCATAAAACCGGCAAGGCGGCCTGCGCCGGTGTTACGTTCCAGCGGGCCGGGGATGACGCCGATTTCGCGGTGACCGCGTTCAATCAGATAACGCCCGGCCATGTAGCCGCCTTCGAACGCGTTATCAATGACCGCATCGGTGAAGTCAGCTTTTGCTTCACCCCAGTCCATGACCACCATTGGGATATGGCGATACTCTTCCAGCATCGCC  >  DH1/2139950‑2140242

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑