Predicted mutation
evidence seq id position mutation annotation gene description
RA DH1 4,547,816 A→G Y80H (TAC→CAC)  rho ← transcription termination factor Rho

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH14,547,8160AG98.4% 1417.1 / 4.9 377Y80H (TAC→CAC) rhotranscription termination factor Rho
Reads supporting (aligned to +/- strand):  ref base A (4/2);  new base G (179/191);  total (183/194)
Fisher's exact test for biased strand distribution p-value = 4.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.97e-01

Only 100 of 377 total aligned reads displayed.

TGCGGGCGTTTTCAGGTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAAATAATGTCCTGCTT  >  DH1/4547669‑4547963
                                                                                                                                                   |                                                                                                                                                   
tgCGGGCGTTTTCAGGTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGa                                                                                                                                                   <  8:487560/150‑1 (MQ=255)
    ggCGTTTTCAGGTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGtca                                                                                                                                              <  4:144101/151‑1 (MQ=255)
    ggCGTAATCAGCTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCCATCACCTTCTTTCGGCGCGCGAATCTTACCAGACATGGTATCACCAGTGCGGAGGTTGAAAGGGCGCATTTGGCTAGGGGAAACGTGGATGtc                                                                                                                                               >  4:311911/1‑150 (MQ=255)
     gCGTTTTCAGGTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGtcat                                                                                                                                             <  1:1225873/151‑1 (MQ=255)
      cGTTTTCAGGTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGtcatc                                                                                                                                            <  6:1141999/151‑1 (MQ=255)
      cGTTTTCAGGTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGtcatc                                                                                                                                            >  4:696721/1‑151 (MQ=255)
       gTTTTCAGGTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGtcatc                                                                                                                                            >  4:245473/1‑150 (MQ=255)
         tttCAGGTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAgg                                                                                                                                         >  6:1271377/1‑151 (MQ=255)
         tttCAGGTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAg                                                                                                                                          <  6:648362/150‑1 (MQ=255)
           tCAGGTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGa                                                                                                                                        <  7:81919/150‑1 (MQ=255)
            cAGGTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTTCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGAc                                                                                                                                       <  7:766036/150‑1 (MQ=255)
            cAGGTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGAcc                                                                                                                                      >  1:202070/1‑151 (MQ=255)
            cAGGTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGAc                                                                                                                                       <  7:173070/150‑1 (MQ=255)
            cAGGTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGAc                                                                                                                                       <  4:1191039/150‑1 (MQ=255)
            cAGCTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGAcc                                                                                                                                      <  8:335066/151‑1 (MQ=255)
             aGGTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCg                                                                                                                                     >  2:1085535/1‑151 (MQ=255)
              ggTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCgg                                                                                                                                    >  3:1025829/1‑151 (MQ=255)
              ggTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCgg                                                                                                                                    <  5:878229/151‑1 (MQ=255)
               gattctCGAAGTTAACTTCGATAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCCGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATATGGCAAGGGGAAACGTGGATGTCATCAGGACCGGc                                                                                                                                   >  8:1299542/6‑151 (MQ=255)
               gTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGc                                                                                                                                   <  7:1405072/151‑1 (MQ=255)
                tttGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCg                                                                                                                                  >  5:525772/1‑151 (MQ=255)
                  tGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGa                                                                                                                                 <  7:281329/150‑1 (MQ=255)
                        aGTTAACTTCGTTAACTTTCAGCAGCGCAATATAGCGTTGACCTTCTTTCGGGGGGCGAATCTTACCAGAGATGGTATCACGAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGa                                                                                                                          <  6:727178/151‑1 (MQ=255)
                        aGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAgg                                                                                                                           >  5:1187154/1‑150 (MQ=255)
                         gTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAg                                                                                                                         <  2:380806/151‑1 (MQ=255)
                         gTTAACTTCGTTAACTTTCAACAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAgg                                                                                                                           >  3:254182/1‑149 (MQ=255)
                          ttAACTTCGTTAACTTTCAGCAGCGCAATATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGTTGAAACGTGGATGTCATCAGGAACGGCGAGGTAGGAGc                                                                                                                        >  8:1219986/1‑151 (MQ=255)
                            aaCTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCTAGGTAGGAGCtt                                                                                                                      >  7:1236709/1‑150 (MQ=255)
                            aaCTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCt                                                                                                                       <  6:703598/150‑1 (MQ=255)
                              cTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGtg                                                                                                                    >  2:126875/1‑150 (MQ=255)
                                tCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTc                                                                                                                    <  3:1376613/149‑1 (MQ=255)
                                tCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCt                                                                                                                   <  3:149171/150‑1 (MQ=255)
                                tCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGAGTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTg                                                                                                                  >  4:1274914/1‑151 (MQ=255)
                                 cGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTg                                                                                                                  <  5:1071488/150‑1 (MQ=255)
                                 cGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGc                                                                                                                 <  7:956495/151‑1 (MQ=255)
                                  gTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCg                                                                                                                >  1:1152992/1‑151 (MQ=255)
                                   ttAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCgg                                                                                                               >  1:538587/1‑151 (MQ=255)
                                       cTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGCGGTAGGAGCTGTCTGCGGAACg                                                                                                           >  3:382338/1‑151 (MQ=255)
                                           cagcagCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACggagg                                                                                                       <  3:224074/151‑1 (MQ=255)
                                           cagcagCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACggagg                                                                                                       >  7:359624/1‑151 (MQ=255)
                                                gcgcAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAAcc                                                                                                  <  5:24322/151‑1 (MQ=255)
                                                    aaaaTAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGTAACGTGGATGTCATCAGGACCCGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAat                                                                                              >  5:1178278/1‑151 (MQ=255)
                                                      aaTAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAatcc                                                                                            >  8:914909/1‑151 (MQ=255)
                                                       aTAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTGATCAGGTCCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAatcc                                                                                            >  6:1426006/1‑150 (MQ=255)
                                                       aTAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAatcca                                                                                           <  1:1095874/151‑1 (MQ=255)
                                                       aTAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAatcc                                                                                            >  1:641213/1‑150 (MQ=255)
                                                          gCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAatccatcc                                                                                        <  1:1320854/151‑1 (MQ=255)
                                                            gTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTg                                                                                      >  3:994940/1‑151 (MQ=255)
                                                             ttCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGc                                                                                     >  6:790533/1‑151 (MQ=255)
                                                              tCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGTAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGc                                                                                     <  8:1121452/150‑1 (MQ=255)
                                                               cACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCa                                                                                    <  8:655242/150‑1 (MQ=255)
                                                                aCCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAat                                                                                  <  7:521101/151‑1 (MQ=255)
                                                                  cttcttTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAatat                                                                                >  3:504729/1‑151 (MQ=255)
                                                                     cttTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATAtctc                                                                             >  4:901325/1‑151 (MQ=255)
                                                                      tttCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTcc                                                                            >  1:372437/1‑151 (MQ=255)
                                                                      tttCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTcc                                                                            >  5:800158/1‑151 (MQ=255)
                                                                      tttCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGAGCCGATCTTGAAACGGCGGATTTGGGTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTGTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTcc                                                                            >  3:75909/1‑151 (MQ=255)
                                                                       ttCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATAtctc                                                                             <  2:794086/149‑1 (MQ=255)
                                                                             gggcgAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGc                                                                     <  6:851806/151‑1 (MQ=255)
                                                                             gggcgAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGc                                                                     <  5:163771/151‑1 (MQ=255)
                                                                              ggcgAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGcc                                                                    >  6:986940/1‑151 (MQ=255)
                                                                              ggcgAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGcc                                                                    <  2:91825/151‑1 (MQ=255)
                                                                                 gAATCTTACGAGAGATGGTATGAGCAGTGCGGAGGGTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATc                                                                 <  6:473825/151‑1 (MQ=255)
                                                                                   aTCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCAc                                                               >  2:677306/1‑151 (MQ=255)
                                                                                     cTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCAcc                                                              >  3:630997/1‑150 (MQ=255)
                                                                                           agagATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCCTCAGGACCGGCGAGGTAGGAGCGGTCTGCGGCACGGAGGACGCCAACTCCATCCTGCACTATCTCCAGTACGCCATCACCAAAGat                                                        >  2:85495/1‑150 (MQ=255)
                                                                                               aTGGTATCAGCAGTGCGGAGGTTGATACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGTCGAGGTAGGAGCGGTCTGCGGAAAGGAGGAAACCATATCCATCCTGCAATATCTCCAGTAGGCCATCACCAAAGATATCtt                                                   <  7:1299249/151‑1 (MQ=255)
                                                                                               aTGGTATCACCAGTGCGGAGGTTGAAACGGCGGAGTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCtt                                                   <  6:1026718/151‑1 (MQ=255)
                                                                                                 ggTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCg                                                 <  4:471973/151‑1 (MQ=255)
                                                                                                  gTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGc                                                <  8:1009484/151‑1 (MQ=255)
                                                                                                    aTCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGcc                                               >  2:1422238/1‑150 (MQ=255)
                                                                                                      cACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCAct                                            >  3:1096143/1‑151 (MQ=255)
                                                                                                      cACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCAc                                             <  7:1077768/150‑1 (MQ=255)
                                                                                                      cACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATATTCGCCAct                                            >  2:203931/1‑151 (MQ=255)
                                                                                                          aGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAATCGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCttt                                        <  2:64156/151‑1 (MQ=255)
                                                                                                          aGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCttt                                        <  6:313077/151‑1 (MQ=255)
                                                                                                          aGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCttt                                        <  3:421373/151‑1 (MQ=255)
                                                                                                           gTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTg                                       <  3:1239259/151‑1 (MQ=255)
                                                                                                             gCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGGCATCAGGACCGGCGAGGGAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGc                                      <  1:286232/150‑1 (MQ=255)
                                                                                                              cGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGt                                    <  1:896694/151‑1 (MQ=255)
                                                                                                                  ggTTGAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGtgctg                                <  4:1123724/151‑1 (MQ=255)
                                                                                                                      gAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCtt                             <  5:1311874/150‑1 (MQ=255)
                                                                                                                      gAAACGGCGGATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTc                            <  8:141476/151‑1 (MQ=255)
                                                                                                                         aCGGGGGATTTGGCTAGGTGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCTCCACTCTTTGCGTGCTGCTTCAgg                         <  2:1401913/151‑1 (MQ=255)
                                                                                                                          cggcggATTTGGCTAGGGGAAAGGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGa                        <  4:621678/151‑1 (MQ=255)
                                                                                                                           ggcggATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGAt                       >  5:1419442/1‑151 (MQ=255)
                                                                                                                             cggATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATgg                     >  1:1304436/1‑151 (MQ=255)
                                                                                                                              ggATTTGGCTAGGGGAAACGTGGATGTCATC‑GGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGGTTCAGGATGGc                    >  1:153832/1‑150 (MQ=255)
                                                                                                                               gATTTGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCa                   <  8:1240194/151‑1 (MQ=255)
                                                                                                                                aTTTGGCTAGGGGAAACGTGGAGGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCaa                  <  2:314485/151‑1 (MQ=255)
                                                                                                                                   tGGCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGAACGCCATCACCAAAGATATCTTCCCCACACTTTGCGTGCTGCTTCAGGATGGCaaaa                >  2:576966/1‑150 (MQ=255)
                                                                                                                                   tGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCaaaa                >  5:698113/1‑150 (MQ=255)
                                                                                                                                    ggCTAGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAaat              >  1:1040088/1‑151 (MQ=255)
                                                                                                                                     gCTAGGGGAAACGGGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAaata             <  2:451384/151‑1 (MQ=255)
                                                                                                                                        aGGGGAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCGGGATGGCAAAaataa            <  6:1346326/149‑1 (MQ=255)
                                                                                                                                         ggggAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAAATAATGt         <  1:982925/151‑1 (MQ=255)
                                                                                                                                           ggAAACGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACAAAAGATATCTTCGCCACTCTTTGCGTGCTGATTCAGGATGGCAAAAATAATGTc        >  2:1318555/1‑150 (MQ=255)
                                                                                                                                              aaCGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAAATAATGTCCTGc    <  6:1371448/151‑1 (MQ=255)
                                                                                                                                              aaCGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAAATAATGTCCTGc    >  4:342511/1‑151 (MQ=255)
                                                                                                                                                cGTGGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAAATAATGTCCTGCtt  <  8:407835/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TGCGGGCGTTTTCAGGTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAAATAATGTCCTGCTT  >  DH1/4547669‑4547963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑