Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1613,6130TG25.5% 241.7 / 35.4 225intergenic (+474/+549)garK/yhaCglycerate 2‑kinase/pentapeptide repeats‑related protein
Reads supporting (aligned to +/- strand):  ref base T (60/101);  new base G (1/54);  total (65/160)
Fisher's exact test for biased strand distribution p-value = 1.94e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 227 total aligned reads displayed.

GGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCC‑GCTTCG‑GCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCACGACGC‑TATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCG  >  DH1/613463‑613753
                                                                                                                                                         |                                                                                                                                             
  tAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCC‑GCTTCG‑GCGGGTTTTTGCTTTTG‑GTGTGCCAGCACGATTAATCACAGttc                                                                                                                                             >  1:608432/1‑149 (MQ=25)
   aGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCC‑GCTTCG‑GCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTcc                                                                                                                                             <  7:321564/150‑1 (MQ=255)
    ggCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCC‑GCTTCG‑GCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAAAGACTGTCCac                                                                                                                                           <  2:579384/151‑1 (MQ=255)
     gctgctTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCC‑GCTTCG‑GCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCacg                                                                                                                                          <  5:879532/151‑1 (MQ=255)
       tgctTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTAACCTGATTTACATAAAAACCC‑ACTTCG‑GAAGGTAATTCGTTTTG‑GAATGAGAGAAACAAGAATGACTGTCCaacac                                                                                                                                        >  4:92778/1‑147 (MQ=255)
       tgctTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACACAAACCGCCTTATCATACACTTTCACAAGATTTACGTAAAAAACA‑TCTTCT‑TAGTGTTTTTCAATTTG‑GAGGGGCAAAAAGATAAATAACTATCCacgac                                                                                                                                        >  6:1012231/1‑151 (MQ=255)
       ggctTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCC‑GCTTCG‑GCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCacgac                                                                                                                                        <  6:808824/150‑1 (MQ=255)
       ggctTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCC‑GCTTCG‑GCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCacgac                                                                                                                                        <  7:1001859/150‑1 (MQ=255)
        gctTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCC‑GCTTCG‑GCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCacgac                                                                                                                                        <  8:1153854/150‑1 (MQ=255)
        gctTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCC‑GCTTCG‑GCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCacgac                                                                                                                                        <  4:1413988/150‑1 (MQ=255)
        gctTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTAACCGGATTTACGTAAAAACCA‑GCTTCG‑GCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCacgacg                                                                                                                                       >  7:1123225/1‑151 (MQ=255)
           tGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCC‑GATTCG‑GCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCACGACGc                                                                                                                                      <  6:1601701/149‑1 (MQ=255)
            gAGCCAGTTTGGTATTCCTGGCCTATATTAATCACTGTTCTCCCCTTTACCGGGATTATCGGTTAGTTTAACCTTATTTTCGTAAAACCCC‑GCTTCG‑GCGGTTTTTTTTTTTTG‑TAGGGGCACATCGATTAATGACTGTCCACGACGC‑ta                                                                                                                                   <  8:1248067/150‑1 (MQ=255)
             aGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCC‑GCTTCG‑GCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCacgaggggatc                                                                                                                                  >  5:285502/1‑144 (MQ=255)
               ccATTGAGCGTTTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCC‑GCTTCG‑GCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATCAATGACTGTCCACGACGC‑TATAcc                                                                                                                               <  1:1908580/151‑1 (MQ=255)
                    gggCGATTGCGGGCCTAGATGAATGACTGGCCACGACACAACCCGGCTTAGCGGCCAGTTTCACCTGATTTACGTAAAAACCC‑GGTGGG‑GCGGGTTTTTGGTTTTG‑GCGGGGCAGAAAGATCAATGACTGGCCACGACGC‑TATACCCaaaa                                                                                                                          <  3:562399/149‑1 (MQ=255)
                    gAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCC‑GCTTCG‑GCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCACGACGC‑TATACCCaaaa                                                                                                                          <  5:637096/151‑1 (MQ=255)
                    gAGCGATGGCGGGCCTACAGGAATGACTGGCCACGCCAGAACCCGGGTTAGCGGGCCGTTCCACCTGTTTTACGTAAAACCCC‑GCTGCG‑GGGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCACGACGC‑TATACCCaaaa                                                                                                                          <  3:722171/151‑1 (MQ=17)
                     aGCGATGGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCC‑GCTTCG‑GCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGGCCACGACGC‑TATACCCAaaag                                                                                                                         <  7:2059971/151‑1 (MQ=255)
                      gctaTTGCTGGCCTAGATCAATGACTGTCCACGAAAGAACCCGGCTTATCGGTCAGTTTCACCTTATTTACTTAAAAACCC‑GCTTCG‑GCGGTTTTTTGCTTTTG‑GAGGGGCAGAAATATCAATCACTGACCACGACGC‑TATACCCAaaaga                                                                                                                        <  5:1819845/148‑1 (MQ=18)
                        gATTGGTGGCCTATATGAGGGACTGCCCACGACAGAACCGGGCTTATCGGTCTGTTTCACCTTATTTACGTAAAAACCC‑GCTGCG‑GGGGTTTTTTGGTTTTG‑GTGGGCCAGAAAGATTAATGACTGTCCACGACGC‑TATACCCAaaagaaa                                                                                                                      <  7:1451406/151‑1 (MQ=255)
                         aTTGCTGGCCTATTTTAATGACTGTCCACGACAGAACCCGGTTTATGGGTCAGTTTCACCTTATTTAGTTAAAAACCC‑GCTTCG‑GGTGTTTTTTGTTTTTG‑GAGGGGCATAAATATGAATGACTGTCCACGACGC‑TATACCCAaaagaaa                                                                                                                      <  8:865063/150‑1 (MQ=255)
                         aTTGCGGGCCTAGATGAATGACTGGCAACGACAGAACCCGGCTTATCGGTCAGTTTCACGTGATTTACGTAAAAACCC‑GCTTCG‑GCGGTTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCACGACGC‑TATACCCAaaagaaag                                                                                                                     <  1:766770/151‑1 (MQ=255)
                            gCTGGCCTAGATGAATGACTGTCCACGACACAACCCGGCTTATCGATCAGTTTCACCAGATTTACGTAAAAAACC‑GCTTCG‑GCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGAATGTCCAAGACAC‑TATACCCAAAAGAAAGCgg                                                                                                                  >  2:1202141/1‑151 (MQ=17)
                            gCTGGCCTAGATGAATCACTGTCCACGACAGAACCCGGCTTATCGGGCAGTTGCACCTGATTTACGTAAAAACCC‑GCTTCG‑GCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGCCCACGACGC‑TATACCCAAAAGAAAGCg                                                                                                                   <  3:1158012/150‑1 (MQ=255)
                              tGGCCTAGATGAATGACTGTCCAAGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCC‑GCTTCG‑GCGGGTTTTTGGGTTTG‑GAGCGGAAGAAAGATGAAACACAGTAAACCACGC‑TATACAAAAAAGAAAgctgct                                                                                                                >  1:978647/1‑151 (MQ=18)
                              gggCCTAGATCAATGACTGCCCACGACAGACCCCGGCTTATCGGTCAGTTTCACCTTATTTACGTAAAAACCC‑GCTTCG‑GCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCACGACGC‑TATACCCAAAAGAAAGCGGCt                                                                                                                <  4:712883/150‑1 (MQ=255)
                                cccTATATGAATGACTGCCCACGACAGACCCCGGATTACCGGGCAGTTGCACCTGATTTACGTAAAACCCC‑GCTGCG‑GCGGGTTTTTGCTTTTG‑GCGGGGCACAAAGATGAATGACTGCCCACGACGC‑TATACCCAAAAGAAAGCGGCTTa                                                                                                              <  1:2002855/150‑1 (MQ=17)
                                 cctcgaaaacacccTGTCCCCAACATAACGCGGCTTATCGGTCATTTTCACGTGTTTTACTTAAAAACCC‑GCTTCG‑GCGGTTTTTTGGTTTTT‑GAGGGGCACAAAGATTAATGACTGTCCACGACAC‑TATACCCAAAAGAAAGCGGCTTAt                                                                                                             <  1:1201363/138‑1 (MQ=1)
                                  cTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAAACC‑GGTACGACCGCGTTTTAGC‑AATG‑GAGTGCCATAAAGAGGAATGAAGATAGACGGGGC‑TATCCCGAAAAGATAGCCGATTATc                                                                                                            >  7:933702/1‑151 (MQ=12)
                                   tAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACAC‑GCTTCG‑GCGGGGTTTTACTTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCACGACCC‑TATACCCAAAAGAAAGCGGCTTAGCg                                                                                                           >  7:1327862/1‑151 (MQ=255)
                                    aGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCC‑GCTTCG‑GCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCACGACGC‑TGTACCCAAAAGAAAGCgggatcgggg                                                                                                          >  5:1672759/1‑143 (MQ=255)
                                    aGATGAATGACTGGCCCCGACAGAACCCGGCTTTTCGGTCAGTTTCACCTGATTTACTTAAAAACCC‑GCTTCG‑GCGGGTTTTTGCTTTTG‑GTGGGGCTGAAAGATGAATGACTGTCCACGACGC‑TATACCCAAAAGAAAGCGGCTTATCg                                                                                                           <  4:478206/150‑1 (MQ=17)
                                    aGATGAATGACTGCCCACGACAGAACCCGGCTTAGGGGTCAGTTTCACCTGATTTACGTAAAAACCC‑GCTTCG‑GGGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCACGACGC‑TATACCCAAAAGAAAGCGGCTTATc                                                                                                            <  1:297867/149‑1 (MQ=255)
                                     gaggaatgcCTGTCCCCGCCAGAACCCGGATTATCGGTAAGTTTCACCTGATTTACTTAAAACCCC‑GCTTCG‑GCGGGTTTTTGCTTTTG‑TAGGGGCAGAAAGATGAATGACTGTCCACGACGC‑TATACCCAAAAGAAAGCGGCTTATCGGt                                                                                                         <  4:921147/148‑1 (MQ=14)
                                     gATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCC‑GCTTCG‑GCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTAGACG‑CGCATATACCCAcaagcaagacccgtctccgt                                                                                                         >  5:1916920/1‑139 (MQ=17)
                                      attaatgaCTGTCCACGACAGAACCCGGCTTATGGGTCATTTTCACCTGATTTACTTAAAAACCC‑GCTTCG‑GCGGGTTTTTGTTTTTG‑TTGGGGCACAAATATGAATGACTGTCCACGACGC‑TATACCCAAAAGAAAGCGGCTTATCGGTc                                                                                                        <  1:287036/148‑1 (MQ=17)
                                      atgaagttcTGGCCACGAAAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACTAAAAACCCC‑GCTTCG‑GGGGTTTTTTGCTTTTG‑GAGGGGCACAAACATGAATGACTGGCCACGACGC‑TATACCCAAAAGAAAGCGGCTTATCGGTc                                                                                                        <  1:356127/143‑1 (MQ=14)
                                       tgaatgaCTTTCCACGACAGAACCCCGCTTATCGGCCAGTTTCACCTGATTTACTTAAAACCCC‑GCTTGG‑GGGGGTTTTTGCTTTTG‑GAGGGGCAGAAATATGAATGACTGTCCACGACGC‑TATACCCAAAAGAAAGCGGCTTATCGGTc                                                                                                        <  2:1125064/150‑1 (MQ=17)
                                       tgaatgaCTGCCCCCCAACCAACCCGCCTTACCGGTCATTTTCACCTGTTTTACGTAAAACCCC‑GCTTCG‑GCGGTTTTTTGCTTTTG‑GTGGGGCATAAAGTTGAATCACTGTCCACGACGC‑TATACCCAAAAGAAAGCGGCTTATCGGTc                                                                                                        <  2:225082/150‑1 (MQ=11)
                                        accagcgtaacccaaacacAACCCGGCTTATGGGCCTGTTTCACCTGTTTTACTTAAAACCCC‑TCTTCG‑GCGGGTTTTTGGTTTTG‑CTGGGGCACAAAGATGAATGACTTGCCACGACAC‑TATACCCAAAAGAAAGCGGCTTATCGGTCAg                                                                                                      <  7:1948754/136‑1 (MQ=2)
                                          atgaCTGGCCACGACAGAACCCGGCTTATGGGGCAGTTTCACCTGATTTACGTAAAAACCC‑GCTGCG‑GCGGTTTTTTGGTTTTG‑GAGGGGCATAAAGATGAATGACTGTCCACGACGC‑TATACCCAAAAGAAAGCGGCTTATCGGTCAGtt                                                                                                    <  3:917626/151‑1 (MQ=17)
                                            gacggcccaccaCAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACTTAAAACCCC‑GCTTCG‑GCGGGTTTTTGGTTTTG‑TTGGGGCACAAATATGAATGACTGGCCACGACGC‑TATACCCAAAAGAAAGCGGCTTATCGGTCAGttt                                                                                                   <  6:766661/144‑1 (MQ=14)
                                            gaatGTCCACGTCATAAGCCGTCTTATGGGTAATTTTGTCGTGATTTACTTAATAACGG‑GCTTCG‑GCGGGTTTTTGTTTTTG‑TTGGGGCATAAATATGAATGACTTTCCACGACTC‑TATACCCAAAAGAAAGCGGCTTATCGGTCAGttt                                                                                                   <  8:1640164/147‑1 (MQ=2)
                                             aCTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTGACCTGATATAACTAAAAACCC‑TCTTCT‑ACGGGATTATGCTTTAG‑CAGAGGCAAAAAGATGAATGACTGACCAAGAATA‑TATAAAAAAAAGAAAGCGGCTTATCGGTCAGtttt                                                                                                  >  4:346502/1‑149 (MQ=9)
                                             aCTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTAACCTGATTTACATAAAAACAC‑CCTTCG‑GAAGGTATTTACTTTTT‑AAATTACAGAAAAAATAATTACTCTCCAAGAAAA‑TATAAAAAAAAGAAAGAGGCTTATCGGTCAGtttg                                                                                                  >  8:541873/1‑149 (MQ=255)
                                                 gccACGACAGAACCCGGCTTATCGGTCAGTTGCACCTGATTTACGTAAAAACCC‑GCTTCG‑GCGGGTTTTTGCTTTTG‑TAGGGGCACAAAGATTAATGACTGTCCACGACGC‑TATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTg                                                                                             <  3:526245/150‑1 (MQ=255)
                                                 cccaccaCACAACCCGGCTTATCGGGCAGTTTCACCTGATTTACGTAAAAACCC‑GCTTCG‑GCGGGTTTTTGTTTTTG‑TCGGGGCACAAAGATGAATGACTGTCCACGACGC‑TATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTg                                                                                             <  5:1098830/150‑1 (MQ=14)
                                                  ccACGACAGAACCCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAACCC‑GCTACG‑GCGGGTTTATGCTTTTT‑GAGTGCCATAAACATAAATGACTCTACACGCCAC‑TAAAACCAAAAAAAAGATTATTATAGTTCAGTTTAACCtt                                                                                             >  8:1879078/1‑149 (MQ=255)
                                                   cacaACAGAACCCGGCTTATCGGGCAGTTTCACGTGATTAACGTAAAACCCC‑GCTGCG‑GCGGGTTTTTGGTTTTG‑CTGGGGCACAAACATGAATGACTGGCCACGACGC‑TATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGt                                                                                           <  7:1885425/151‑1 (MQ=14)
                                                    ccGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCC‑GCTTCG‑GCGGGTTTTTGGTTTTG‑GAGGGGCAGAAAGATGAATGACTTTCCACGACGC‑TATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGtt                                                                                          <  7:1660886/150‑1 (MQ=255)
                                                    accacacAACCCGGCTTATCGGACACTTGCACCTCATTTACTTAAAACCCC‑GCTTCG‑GCGGGTTTTTGCTTTTG‑GAGGGGCAGAAACATGAATCCCTGTCCACGACGC‑TATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGtt                                                                                          <  7:842990/148‑1 (MQ=14)
                                                    aCGACAGAACCCGGCTTATCGGTCAGTTTCAACTTATTAACATAAAAACAC‑TCATCT‑GCAGTGACTTTCATTTG‑TATGGGAAGCATGATTATTCAGTGTAAAATAAGC‑TATCGCCAAAAGACAGCGGCTTCTCGGTGCGTTTCaggtgggg                                                                                          >  5:2074766/1‑144 (MQ=255)
                                                     ctactgAACCCGGCTTATGGGGAAGTTTCACGTGATTTACTTATAACCCG‑GCTTCG‑GCGGGTTTTTGTTTTTG‑TTGGGTCTCAAATATGAATGACTGTCCACGACGC‑TATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGt                                                                                           <  2:1813345/144‑1 (MQ=14)
                                                      caCAGAACCCGGCTTATCGGGAATTTTCACGTGATTTACTTAAACACCC‑GCTGCG‑GCGGGTTTTTGCTTTTG‑GAGGGGCATAAAGATGAATGACTGGCCACGACGC‑TATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTa                                                                                        <  1:1126254/150‑1 (MQ=14)
                                                       ccAGAACCCGGCTTAGCGGGCAGTTGCACCTGATTTACGTAAAAACCC‑GCTGCG‑GCGGGTTTTTGCTTTTG‑GCGGGGCCGAACGATGAATGCCTGGCCACGACGC‑TATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTAc                                                                                       <  3:253761/150‑1 (MQ=14)
                                                           aaCCCGGATTATGGGGAATTTTCCCTTCATTTACTTAACCACCT‑TCTGAT‑GCGGGTTTTTGTTTTTG‑TTGGCGTTTAACTATGAATGACTTGCCAAGACTC‑TATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTaa                                                                                   <  2:1892823/151‑1 (MQ=2)
                                                             cccGGCTTATCGGGCAGTTTCACCTGATTTACGTAAAACCCC‑GCTGAG‑GCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAAGGACTGTCCACGACGC‑TATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTaaaa                                                                                 <  1:85638/151‑1 (MQ=255)
                                                               cGGCTTCTGGGTCAGTTTCACCTCATTTCGGTAAAAACCG‑GCTGCG‑GGGGGTTTTTGGTTTTG‑GTGGGGACCAAAGATGATTGACTGGCCACGACGC‑TATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAAc                                                                               <  3:1946531/151‑1 (MQ=11)
                                                               cGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCC‑GCTTCG‑GCGGGTTTTTTCTTTTC‑GAGGGTCAGAAACATTATTAACTCAAGACGAATG‑GATAAACAAAAGAAGGCGGCTTATGGGTCAGTAAAACCTGGTTTACGTaaactc                                                                               >  3:666835/1‑148 (MQ=2)
                                                                   ttATCGGTCAGTTTCACCTGATTTACGTAAAAAAAC‑GCTTCG‑GCGGGGTTTTGCTTTTG‑GAGGGGAAGAAAGATGAATGACTGTCCACGACGC‑TATCCAAAAAAGAAAGCGGCTTATCGGTCAGTGTGACCTGGTGTACGTaatagctcgg                                                                           >  5:1190879/1‑143 (MQ=11)
                                                                    aatggttaaGTTTCCCGTGATTTACGTAAAACCCG‑TCTTAG‑GCGGGTTTTTGCTTTTG‑TTGGTGCTAAATTTTGAATGACTTTCCACGACGC‑TATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGc                                                                           <  4:1637051/142‑1 (MQ=11)
                                                                     atgggTAAGTTTCCGGTGATTTACTTAAAAACCG‑GCTTCG‑GCGGGTTTTTGTTTTTG‑TTGGGGCACAAATATGAATGACTTGCCACGACGC‑TATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCtt                                                                         <  8:1944580/148‑1 (MQ=11)
                                                                     aaggggaaatttCCCCTGATTTCCGTAAAAACCG‑CCTTCC‑GCGGGTTTTTGTTTTTGCGCGGGGCATAAA‑TTTAATTCCTTGCCACGACGC‑TATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCt                                                                          <  1:2036406/141‑1 (MQ=2)
                                                                       cGGTCAGTTTCACCTGATTTACGTAAAAACCC‑GCTTCG‑GCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCACGACAC‑TATACCCAAAAGAAAGCGACATATCAATAACTTACACATGGTAAACATaaaaaaaatatt                                                                         >  8:119954/1‑142 (MQ=11)
                                                                         ggaaattgcACGTGATTTAGGTCTAAACCC‑GCTTCG‑GCGGGTTTTTGATTTTG‑GCGGGGCCCAACCATGAATGCCTCGCCACGACGC‑TATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTcggc                                                                     <  3:1126976/143‑1 (MQ=11)
                                                                          tCAGTTTCACCTGATTTACGTAAAAACCC‑GCTTCG‑GCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCACGACGC‑TATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGGTGTACGTAATAACCCGGTGcgggg                                                                    >  1:488576/1‑149 (MQ=11)
                                                                           aatttgcACGTGATTTAGGTAAAAACCT‑GCTTCT‑GCGGGTTTTTGTTTTTG‑CTGGTGACCAAATATGATTGACTGTCCACGACGC‑TATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTcggcgg                                                                   <  1:509307/145‑1 (MQ=11)
                                                                            attttctggtGATTTTCTAAAAACCCC‑TGTTCG‑GCGGTTTTTTGTTTTTG‑AGGGTGCTAATTATTGATTGACTTGACTCGTCTC‑TATACCCAAAAGAACGCGGCTTATCGGTCAGTTTCACCTTGTTTACGTAAAAACCCGCTTCGGCggg                                                                  <  6:1822053/142‑1 (MQ=2)
                                                                            aGTTTCACCTGATTTACGTAAAAACCC‑GATACG‑GCATCTTTTTACTTTTG‑TAGGGGCAGAAAGATGAATGAATATACAAGAAAC‑TATACCAAAAAGAAAGATTAATATAGGTCAGTTTAACCTGTTTTACGTAATAAACCGTTCcgggctg                                                                  >  2:669474/1‑147 (MQ=255)
                                                                            aGTTTCACCTGATTTACGTAAAAACCC‑GATAAG‑AACGGATTTTCCTATTG‑GAGGGACAGAAAGATTAATTAATGTCAAAGAAAC‑TATACCCAAAAGAAATAAAATTATAGGTCAGTTTTACCTGGTTTACGTAATAAACCgttcaggtcgg                                                                  >  4:1178952/1‑141 (MQ=255)
                                                                             gTTTCACCTGATTTCCGAAAAACCCC‑GCTGCG‑GCGGGTTTTTGCTTTTG‑GAGGGGCAGAAACATGAATGACTGGCCACGACGC‑TATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCgggc                                                                 <  5:2015485/151‑2 (MQ=11)
                                                                             gTTTCACCTCATTTACTTAAAACCCG‑GCTGCG‑GCGGGTTTTTGGTTTTG‑GCGGCGCATAAACATCAATGACTGTCCACGACGC‑TATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGt                                                                 <  7:455870/151‑1 (MQ=11)
                                                                               ttCACCTGATTTACGTAAAAACCC‑GCTTCG‑GCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCACGACTC‑TATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAAACCACATcggaag                                                                   >  8:174726/1‑144 (MQ=11)
                                                                                tCACCTGATTTACGTAAAAACCC‑GCTTCG‑GCGGGTTTTTGCGTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCACGACTC‑TATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGCTGTCCGTAATAACGCTCGGCGGCggggagg                                                              >  5:1301857/1‑147 (MQ=11)
                                                                                 gccatGATTTCCGTAACAACCG‑CCTTCG‑GCGGTTTTTTGTTTTTG‑TCGGGGCTTAATTATGATTGACTGGCCACGACGC‑TATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGtttt                                                              <  6:1268240/146‑1 (MQ=11)
                                                                                  aCCTGATTTACGTAAAAACCC‑GCTTCG‑GCGGGTTTTTGCTTTTG‑GCGGGGGAGAAAGATGAATGACTGTCCCCGACGC‑TATAACCAAAAGAAAGCGGCTTATCGGTCAGTTTCACATGGTTTACGTAAAAACCCGGGGCGGCGGGTTTTTg                                                            >  1:1383396/1‑151 (MQ=11)
                                                                                    gtgatattcttAAACAGCC‑GCTGCT‑GCGGGTTTTTGTTTTTG‑TTTGGGCTCAAATATGAATGACTCTCCACGACGC‑TATACCCTAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCt                                                          <  6:2012570/141‑1 (MQ=11)
                                                                                    cTGATTTACGTAAAAACCC‑GCTTCG‑GCGGCTATGTGATGTTC‑GAGGGGCTGAAAGAGGAATGCCTGGACACGACGC‑TTTACCCATAAGAACGCTGCTAATAGCTCAGTGGGACCGGGTGGACGTAAACTGCCGCGTGGGCgggggtggggg                                                          >  7:517317/1‑143 (MQ=2)
                                                                                     tcatatagttAAAAACCC‑GCTTCG‑GCGGGTTTTTGCTTTTG‑GCGGTGCTCAAAGATGATTGACTGTCCACGACGC‑TATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCt                                                          <  8:434124/141‑1 (MQ=11)
                                                                                     gcattaccgaaacccccc‑cCTGCG‑GCGGGTTATTGCGTTTG‑CCCGCGCACAAACATGAAGGACTCGCCACGACGC‑TATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCtt                                                         <  5:1322971/136‑1 (MQ=11)
                                                                                      gATTTACGTAACAACGG‑GCTTCT‑GCGGGTTTTTGCTTTTG‑TTGGCGCTTAATTTTGAATGACTGTCCACGACGC‑TATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCtt                                                         <  4:469169/150‑1 (MQ=11)
                                                                                       aTTTCCGTAAAACCCC‑GCTTCG‑GCGGGTTTTTGATTTTG‑CTGGTGCACAATTATGATTGACTTTCCACGACTC‑TATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCtttt                                                       <  2:936014/151‑1 (MQ=11)
                                                                                       aTTTACGAAAACCCCC‑CCTGCG‑GCGGGTTTTTGCTTTTG‑CCGGGGCCCAAACATGAAGGACTCGCCACGACGC‑TATACCCAACAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCtttt                                                       <  5:81686/151‑1 (MQ=11)
                                                                                         ttACGAAACACCCC‑GCTGAG‑GCGGGTTTTTGCTTTTG‑ACGGTGCACAACCTTGAATGACTGTCCACGACGC‑TATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTg                                                      <  2:408067/150‑1 (MQ=11)
                                                                                           acgcaaccaCCC‑GCTGCG‑GCGGGTTTTTGCTTGGG‑GCGGGGCAGACCGAGGAATGCCGGGCCACGACGC‑TCTACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTGCGGCGGGTTTTTGCTTTTggc                                                    <  1:1436469/142‑2 (MQ=11)
                                                                                            gttAAAAACCT‑GCTTCG‑GGGGGTTTTTGCTTTTG‑TTGGTGCTTAATCATGATTGACTGTCCACGACGC‑TATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAgg                                                  <  2:1476894/149‑1 (MQ=11)
                                                                                              tAAAAACCC‑TGTTCG‑GGGGGTTTTTGCTTTTG‑GTGGTGCAGAATGATGATTGACTGGCCACGACGC‑TATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAggg                                                 <  6:1922873/150‑1 (MQ=11)
                                                                                              gaaacaCCCGGCTTCG‑GCGGGTTTTTGCTTTTG‑GAGGGGCACAAAGATGAATGACTGGCCACGACGC‑TATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAggg                                                 <  5:940389/150‑1 (MQ=255)
                                                                                                 aaaCGC‑CCTGCG‑GCGGGTTTTTGTTTTTG‑CTGGTGTTTAACCTTGAATGCCTTTCCACGACGC‑TATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAg                                             <  4:1965705/151‑1 (MQ=11)
                                                                                                     cc‑GCTTCG‑GCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCACGACGC‑TATACACAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAATAACCAGATTCGGCGGGTTTATGATTTTTGAgtgggatttat                                         >  5:372096/1‑144 (MQ=11)
                                                                                                       gtcTTCG‑GGGGTTTTTTGCTTTTG‑GAGGGGCTTTCTTATGATTGAGTCTCCACGACGC‑TATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGa                                        <  4:236426/149‑1 (MQ=11)
                                                                                                         gctgccgcggGTTATTGTTTTTG‑TTGGTGCTCAATTATGAATGACTTGACTAGACTC‑TATACACAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGat                                       <  2:51513/145‑1 (MQ=11)
                                                                                                           gcg‑gcggGTTTTTGTTTTTG‑GTGGAGGCTAATCATGAATGACTCGACACGACGC‑TATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGatg                                      <  3:1507329/148‑1 (MQ=11)
                                                                                                             g‑gcggGTTTTTGGTTTTG‑CCGGGGTTCTACCTTGAATGACTCGCCACGACGC‑TATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGatgaatg                                  <  5:1461600/151‑1 (MQ=11)
                                                                                                                cggGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCACGACAC‑TATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAAACCGCTGCGGCGGGTTTTAGATTTTTGAGTGGGATaaataacaccgcg                                >  1:1402908/1‑141 (MQ=11)
                                                                                                                   gTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCACGACGC‑TATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGATTTACGTAAAAAACCGATCCGGCGGGTTTATGATTGTTGAGTGGGAGtaattaatgagg                                 >  1:1852385/1‑138 (MQ=11)
                                                                                                                     ttttGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCACGACGC‑TATACCCAAAAGAAAGCGGCTTATCGGTCAGtttta                                                                                                 >  1:1345388/1‑79 (MQ=18)
                                                                                                                      tttGTTTTTG‑CTGGGGGTCAAATATGAATGACTAGCTAATACGC‑TCTACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGTGGCAGAAAGATGAATGACTGTcc                           <  3:132983/150‑1 (MQ=11)
                                                                                                                                            aGATGAATGACTGTCCACGACGC‑TATACCCAAAAGAAAGCGGCTTATAGATCAGAATAGCGTCGTTTACGTAAAAACACTTTACATTAGTTTATATCTATTTGATCGGCAGAAAAATTAAAAAAAATCCACGACACTATACGCAaaaga       >  6:399433/1‑149 (MQ=2)
                                                                                                                                                         |                                                                                                                                             
GGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCC‑GCTTCG‑GCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCACGACGC‑TATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCG  >  DH1/613463‑613753

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑