BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	DH1	936,169	0	T	.	37.1%	239.9 / 150.6	106	intergenic (‑13/+21)	ECDH1_RS04615/ECDH1_RS04620	tRNA‑Met/tRNA‑Met
Reads supporting (aligned to +/- strand): ref base T (3/63); new base . (2/37); total (5/101)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.93e-01

Only 100 of 131 total aligned reads displayed.

GAATGGCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATT  >  DH1/936019‑936318

gAATGGCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCt                                                                                                                                                        <  5:420316/151‑1 (MQ=255)

gAATGGCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCAGGTGTTAAACCGACCCGGGTCCGCTTATGAGCCCGTCGCGATTCGCGTATGCTCCACGCCGCGTGACAGTAATGCt                                                                                                                                                        >  7:945501/1‑151 (MQ=255)

 aaTGGCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCtt                                                                                                                                                       <  5:1372910/151‑1 (MQ=255)

 aaTGGCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCtt                                                                                                                                                       <  6:1968315/151‑1 (MQ=255)

 aaTGGCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCtt                                                                                                                                                       <  4:1704791/151‑1 (MQ=255)

 aaTGGAGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCt                                                                                                                                                        <  4:123576/150‑1 (MQ=255)

  atgggtcTTTTTTATGGGAGACCTGAAACAAAAAATCACGTGTTAGGGTGTTCAATTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCttt                                                                                                                                                      <  8:1996033/145‑1 (MQ=18)

  atgcgccTTTTTTTTGGGACACCCCAAACAAAAAATGAGGGGTAATGCTGTACATTCATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCttt                                                                                                                                                      <  1:571571/145‑1 (MQ=11)

  aaggCTCTTTTTTATTGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCttt                                                                                                                                                      <  4:701768/149‑1 (MQ=255)

  aTGGCGCTTTTTTATGGGAGACCAGAAACAAATAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCGCGCGTCACCGTACTGCttt                                                                                                                                                      <  4:1994380/151‑1 (MQ=255)

  aTGGCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCttt                                                                                                                                                      <  2:463152/151‑1 (MQ=255)

  aTGGCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCttt                                                                                                                                                      <  3:1034528/151‑1 (MQ=255)

    ggatcTTTTTTATGGGAGACCAGAAACAAAAACACACCCGTTAGCTTGATAAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCttta                                                                                                                                                     <  5:1182562/146‑2 (MQ=18)

    ggCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCtttt                                                                                                                                                     <  7:1125133/150‑1 (MQ=255)

    ggCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCtttac                                                                                                                                                    <  4:50080/151‑3 (MQ=255)

    ggCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTa                                                                                                                                                    <  3:278115/151‑1 (MQ=255)

    ggCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTa                                                                                                                                                    <  2:259515/151‑1 (MQ=255)

    ggCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTa                                                                                                                                                    <  6:1708602/151‑1 (MQ=255)

    ggCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTa                                                                                                                                                    <  2:250245/151‑1 (MQ=255)

    ggCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTa                                                                                                                                                    <  3:465347/151‑1 (MQ=255)

    ggCGCGTTTTTAGGGCAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCttta                                                                                                                                                     <  3:499910/150‑2 (MQ=255)

      cgcTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACt                                                                                                                                                  <  5:1458191/151‑1 (MQ=255)

      cgattttttATGTGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACt                                                                                                                                                  <  5:1549514/148‑1 (MQ=255)

       gcTTTTTTATGGGAGACCTGTAACAACAAAACCATCGTTATGGTGTTAATTAATTGGTTGCGGGGGCCTGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTtc                                                                                                                                                <  7:574302/151‑1 (MQ=14)

       gcTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACtt                                                                                                                                                 <  2:647728/151‑1 (MQ=255)

        cgtttttATGGGAGACCCGAACCAAAAAAAGAGCGGATTGGGTGTTCATTCATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCtttactt                                                                                                                                                  <  3:1536027/147‑5 (MQ=14)

        cgtttttATGGGAGACCAGTAAAAACAAAAGACGAGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTtca                                                                                                                                               <  6:1867363/149‑1 (MQ=17)

        cTTTTTTTTTGGAGACCAGATACAAAAAAACACCCTTTTGGTTGTTCAATAATTGGTTGCGGTGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCtttactt                                                                                                                                                  <  1:390098/149‑5 (MQ=17)

         ttttttATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTtca                                                                                                                                               >  2:160382/1‑150 (MQ=255)

          tttttATGGGAGACCAGAAACAAAAAATCAGCCTTTAGGTTGTTCAATTATTGGTGGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTtca                                                                                                                                               <  6:857712/149‑1 (MQ=17)

           ttttttGGGAGCCCAGAAACAAAAAAACACCTGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTtcatc                                                                                                                                             <  4:1890657/150‑1 (MQ=255)

           ttttATGGGATACCAGAAACAAAAAAACACCCGTTAGTGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTtcat                                                                                                                                              <  1:1315147/150‑1 (MQ=255)

           ttttATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTtcat                                                                                                                                              <  8:1510318/150‑1 (MQ=255)

           ttttATGGGAGACCAGAAACAAAAAAACACCCGTTAGGCTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTtcat                                                                                                                                              <  4:1327697/150‑1 (MQ=255)

            tttATTGGAGACCAGCAAAAAAAAATCACCCGTTATGTTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTtcatca                                                                                                                                            <  4:1167236/150‑1 (MQ=17)

             ttATTGGAGACCAGTAACAAAAAAACAGCTTTTAGGTTGTTCAATTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCaa                                                                                                                                           <  2:1135645/150‑1 (MQ=14)

             ttATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTtcatca                                                                                                                                            <  1:747053/150‑1 (MQ=255)

             ttAAGGGAGACCCCAACCAAGAAAACACCCTTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCaa                                                                                                                                           <  3:1843796/150‑1 (MQ=17)

              tATGGGAGACCTGAAAAAAAAAAACACCCTTTAGGTTGTTCAATTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCaaa                                                                                                                                          <  8:41419/150‑1 (MQ=17)

              tATGGGAGACCAGTAACAATAAAAGACCGGTTTGGGTGTTCATTTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCaaa                                                                                                                                          <  1:1012149/150‑1 (MQ=14)

              tATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCaa                                                                                                                                           <  5:1579139/150‑1 (MQ=255)

              tATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCaa                                                                                                                                           <  6:1855273/150‑1 (MQ=255)

              tATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑t                                                                                                                                        >  1:1876670/1‑151 (MQ=255)

               agggcagcccccaaaCAACAAAACCCCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑tt                                                                                                                                       <  7:1869606/139‑1 (MQ=14)

               aTGGGAGACCAGATAAAACAAAACACCCTTCAGGGTGATAAATTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑t                                                                                                                                        <  1:1712177/150‑1 (MQ=14)

               aTGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCaaa                                                                                                                                          <  8:501851/150‑1 (MQ=255)

               aTGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCaaa                                                                                                                                          <  8:408967/150‑1 (MQ=255)

               aTGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑t                                                                                                                                        <  7:468489/151‑1 (MQ=255)

               aTGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑t                                                                                                                                        <  6:1873357/151‑1 (MQ=255)

                ttggAGACCAGTTACAATAAATCACTTGTTTGGTTGTTATTTTATTGGTTGCGGGGGGCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑tt                                                                                                                                       <  4:1639688/148‑1 (MQ=11)

                tGGGAGACCAGCTAAAATTATAGAGCCTTTTGGGTGTTCTTTTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCGCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑tt                                                                                                                                       <  8:1408382/150‑1 (MQ=11)

                tGGGAGACCACAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTCGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑t                                                                                                                                        <  4:483762/150‑1 (MQ=255)

                 gggAGACCAGAATCAAAAAAACACACGTTAGTGTGTTCAATAATTGTTTGCGTGGTCCTGATTTGAACCGACGACCTTCTTGTTATGAGCCCGACGAGCTACCAGGCTGCTGCACCCCGCTTCACCGTACTGCTTTTACTTCATCAAA‑ttt                                                                                                                                      <  1:85351/151‑1 (MQ=18)

                 gggAGACCAGAAACAAAAAAATACCCGTTATGTTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑ttt                                                                                                                                      <  4:88900/151‑1 (MQ=255)

                 gggAGACCAGAAACAAAAAAACACCCTTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑tttt                                                                                                                                     <  3:570460/151‑2 (MQ=255)

                 gggAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑ttt                                                                                                                                      <  1:1224669/151‑1 (MQ=255)

                 gggAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑tt                                                                                                                                       <  1:1169060/150‑1 (MQ=255)

                 gggAGACCACAAAACACTAAACACCCGAGAGGTTGATAAATTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑tttt                                                                                                                                     <  7:1653443/151‑2 (MQ=11)

                 gggACACCAGAAACAAAAAAACACCCGTGAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑ttt                                                                                                                                      <  5:547964/150‑1 (MQ=255)

                 ggcagcccACAAACACAAACACCCCCGTTTGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑ttt                                                                                                                                      <  1:162144/144‑1 (MQ=14)

                  ggAGACCTGAAAAAAAAAAACACCGGTTAGGTTGTTAAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑tttta                                                                                                                                    <  2:1878688/151‑3 (MQ=14)

                  ggAGACCAGAAACAAAAAATCACCCTTTAGGTTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑tttta                                                                                                                                    <  8:666858/151‑3 (MQ=255)

                  ggAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTg                                                                                                                                     <  6:78043/151‑1 (MQ=255)

                  ggACACCTGAAACAAAAAAACACCCTTTAGGGTGTTAAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑tttt                                                                                                                                     <  4:491711/150‑2 (MQ=17)

                   gagaCCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGa                                                                                                                                    <  1:1129367/151‑1 (MQ=255)

                    agaCCTGAAAAAATAAATCACCTTTTATGTTGTTAATTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑ttttaat                                                                                                                                  <  2:2011797/151‑5 (MQ=1)

                    agaCCAGAAACAAAAAAACACCGGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑ttttaat                                                                                                                                  <  2:187354/151‑5 (MQ=255)

                    agaCCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCtt                                                                                                                                                       <  4:117430/132‑1 (MQ=255)

                    agaCCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGAt                                                                                                                                   <  5:126657/151‑1 (MQ=255)

                     gaCCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGAtt                                                                                                                                  <  5:417708/151‑1 (MQ=255)

                     gaCCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGAtt                                                                                                                                  <  4:1972192/151‑1 (MQ=255)

                     ccccAGAAACAAAAAAACACCCGTTAGGTTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑ttttaatt                                                                                                                                 <  5:1346128/149‑6 (MQ=255)

                     caCCAGATAAAATAAAACCCCCGATAGGGTGTTCAATAATTGGGTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑ttttaatt                                                                                                                                 <  1:1909594/150‑6 (MQ=14)

                     caCCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGAtt                                                                                                                                  <  1:523509/150‑1 (MQ=255)

                      aCCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTg                                                                                                                                 <  3:621643/151‑1 (MQ=255)

                      aCCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑ttttaattg                                                                                                                                <  5:898316/151‑7 (MQ=255)

                       ccAGAAACAAATAAAGACTCTTTTTGTTGTTAAATTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑ttttaattgg                                                                                                                               <  8:1609321/151‑8 (MQ=11)

                       ccAGAAACAAAAAAACCCCCGTGAGCGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑ttttaattgg                                                                                                                               <  1:160379/151‑8 (MQ=14)

                       ccAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTgg                                                                                                                                <  3:1410212/151‑1 (MQ=255)

                       ccAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGCGGCCGGAATTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTgg                                                                                                                                <  5:904033/151‑1 (MQ=255)

                       ccACAAACAAAAAAACAGCGTTAATGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑ttttaattgg                                                                                                                               <  1:302851/151‑8 (MQ=11)

                         cgAAACAAAAACACACGCTTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCTGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGtt                                                                                                                              <  3:905743/150‑1 (MQ=11)

                         agtaccAAAAAAACCCCCGTTATGGTGTTCATTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGtt                                                                                                                              <  4:86359/146‑1 (MQ=11)

                         aGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGtt                                                                                                                              <  4:1102553/151‑1 (MQ=255)

                          gtaaCAAAAACTCTCCTTTTAGGTTGTTAATTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTg                                                                                                                             <  4:1720408/149‑1 (MQ=11)

                          gAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTg                                                                                                                             <  6:1248200/151‑1 (MQ=255)

                          gAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTg                                                                                                                             <  2:876042/151‑1 (MQ=255)

                           aaaCAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGCGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTg                                                                                                                             <  2:839234/150‑1 (MQ=255)

                           aaaCAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGc                                                                                                                            <  3:1200651/151‑1 (MQ=255)

                            aaCAAAAACTCACCTTTTATGGTTTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑tttt                                                                                                                                     <  2:1684980/140‑2 (MQ=11)

                            aaCAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCg                                                                                                                           <  7:1382872/151‑1 (MQ=255)

                            aaCAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGCGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCg                                                                                                                           <  2:182496/151‑1 (MQ=255)

                              cAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCggg                                                                                                                         <  3:302311/151‑1 (MQ=255)

                               aaaaaaaCACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGAGGAGCTACCAGGCTGCTCCACCCCGCGTGACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCgggg                                                                                                                        <  4:1816324/151‑1 (MQ=255)

                               aaaaaaaCACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCgggg                                                                                                                        <  3:1224480/151‑1 (MQ=255)

                                aaaaaaCACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCTCCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCggggg                                                                                                                       <  6:1157195/151‑1 (MQ=255)

                                aaaaaaCACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCggggg                                                                                                                       <  1:946063/151‑1 (MQ=255)

                                   acacagCCTTTAGGGTGTTCAATAATTGGTGGCGGGGGGCGGAGGTGCACGGCAGTCGTTCGGGTTATGAGCCCGACGAGCTCCGATGCTTCTCCACCCCGCGTCACCGTACTGCGTTTACTTCCTCAAA‑TTTGATTGGTTGCGGGGGCCg                                                                                                                    <  7:1982609/149‑1 (MQ=17)

                                    aaCAGCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCgg                                                                                                                   <  8:801105/151‑1 (MQ=255)

                                                  tgtTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCtttac                                                                                                                                                    >  7:1111818/1‑103 (MQ=37)

GAATGGCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATT  >  DH1/936019‑936318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`