Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH11,065,6050AT24.9% 342.5 / 112.6 218intergenic (+40/‑23)ECDH1_RS05245/ECDH1_RS05250tRNA‑Arg/tRNA‑Arg
Reads supporting (aligned to +/- strand):  ref base A (96/67);  new base T (38/16);  total (134/84)
Fisher's exact test for biased strand distribution p-value = 1.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 231 total aligned reads displayed.

TCAAAAAATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTAA  >  DH1/1065456‑1065755
                                                                                                                                                          |                                                                                                                                                      
ccAAAAAATCTCCAGGAAACGAAGGTCCCCGATCCATCCGTATCTCTTATGTATACACTCCCCGGATACGAACCGTGTGT‑TTGTACGTTCTCTTCATACCTGAGGCACTATCTCCTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATcag                                                                                                                                                       <  5:1170820/150‑1 (MQ=9)
 cataatttattttttaatgtaaGTTCCCCGTTGCATAGGTAGCTTATCTTTATATATTAATCGGCTACGAACGGTTTGG‑GCTTATGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATcagc                                                                                                                                                      <  8:592348/137‑1 (MQ=9)
   aaaaaTCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATCTCATACTTGATATGCCTATAGTAGCGATATC‑‑AAA‑‑ATcaacac                                                                                                                                                    >  4:339024/1‑150 (MQ=18)
      aaTCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTa                                                                                                                                                  >  3:1206124/1‑150 (MQ=255)
      aaTCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGATTTAGTAGCGGTATC‑‑AAT‑‑ATCATCCGTa                                                                                                                                                  >  4:54260/1‑150 (MQ=255)
           cgatttatgtaatattCCCGATGAAGCGGTAGCTGATCTGTCTAGTGTACTCGGCTACCAA‑CGCGCGGCGCTGCGCTCGGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTaaa                                                                                                                                                <  1:1947128/133‑1 (MQ=9)
                   gttatttgccatttGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACGGAGGGG‑TCTGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCg                                                                                                                                    <  6:990150/138‑1 (MQ=21)
                       gtTTCCCGATGCATCCGTAGCTCAGCTGGATATAGTACTGGGCTACGAACCGAGCGG‑TCGGAGGTTCTAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGc                                                                                                                                <  7:115610/151‑1 (MQ=255)
                       agacgggtgggCATCCGATGTTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGc                                                                                                                                <  1:232799/141‑1 (MQ=25)
                               gagCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGATCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAg                                                                                                                        <  7:432890/149‑1 (MQ=255)
                                tGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAg                                                                                                                        <  7:1024857/150‑1 (MQ=255)
                                 ccagccGTAGCTCAGCTGGATATACTACTCGGCTACGAACGCTGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAg                                                                                                                        <  1:350761/145‑1 (MQ=21)
                                   aTCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTCATCTTCCTTAAGGAGGGGTATC‑‑AAG‑‑ATGAGCAGTAAAATAACTTTGCCGCTGCCTACGTAgagc                                                                                                                     >  3:988715/1‑147 (MQ=17)
                                    tCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATATAATACGTAATATCACATAAGTAGCTATATA‑‑AAA‑‑ATAAGCAGTAAAAAAAAAATCCACAAGCATCCATaaagat                                                                                                                    >  8:432908/1‑145 (MQ=2)
                                          atataGCTGGATAGAGTACTCGGCTACGAACGGTGCGG‑TCGGAGGTTCGAATCCTCGCGGATGCACCATCTCTTACTTTATATGGATTTAGTAGCGGTATA‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGAt                                                                                                             <  2:1944556/147‑1 (MQ=21)
                                           aagagagcgcttccctACTCGGCGACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATa                                                                                                            <  3:227279/136‑1 (MQ=18)
                                               gCTGGATAGAGTACTCGGCTACGAACCGATCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAaaat                                                                                                                                              <  4:1296505/113‑1 (MQ=255)
                                                            cTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATATGTTACTTGATATGGCTTTAGGATCGGTAGG‑‑AAG‑‑ATCTGCAGTAATTTAACTTTGCCGATTCGTCAGTAGCTCAGGGGGATTGACTACTCGGATCCGac                                                                                           >  7:606349/1‑150 (MQ=12)
                                                              cGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATATGGTACTTTATATTCATGAAGGAGCGTTAGC‑‑AAT‑‑ATGAGCAGTAAATTACATTTCCCTATACATACGAAGCAGATCGGGATTGAGTCGTCGGCTGCGAGcc                                                                                         >  1:1051343/1‑151 (MQ=9)
                                                               ggctacttacataGCGT‑TCGGAGGTTAGAATCATCCAGTATTCACTATCTCTTATTTCATATCTCGTTAGTATCGGTATC‑‑TAA‑‑ATCTGCAGTAAATTAACTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCg                                                                                        <  8:316312/139‑1 (MQ=9)
                                                               ggCTACGAACCGTCCGG‑TCGGAGGGTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTag                                                                                                                                                 <  3:1636979/94‑2 (MQ=255)
                                                                 cTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATATTTTACTAAATATTCATAAAGTAGATCTATA‑‑AAT‑‑AACAGGAGTTAAATAAAGTAACAGATTCAAATGTAGATCAGCTGGATAGAGTAcactgaaacgaagcg                                                                                        >  7:929693/1‑135 (MQ=2)
                                                                   acctatctgccGG‑TCGGCGCGTCTAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCg                                                                                    <  7:826903/141‑1 (MQ=14)
                                                                      cgcgcgccGG‑TCGGCGCGTCGAATCCTCGCGTATGCACCATCTCTTACTTGATATCGCTATACTCGCTGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTc                                                                                 <  5:221340/144‑1 (MQ=14)
                                                                        cgtgtcGG‑TCGGAGGTTCTAATCCTCCCGTATGCACCATCTCTTACTTTATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCg                                                                                <  8:74934/145‑1 (MQ=255)
                                                                          gAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAgaataagt                                                                                                                                          >  1:1663131/1‑88 (MQ=255)
                                                                          gAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTAGG‑‑AAG‑‑ATCACCAGTAATAAAACGATGCAGATTCATCAGTAGCGCAGCTGGATATAGTCCTCGGAAAAGACCCGCgcgggcggcg                                                                             >  7:58329/1‑149 (MQ=12)
                                                                             cGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATCTAATACTTCATATGGAGTTAGGAACGGATAC‑‑AAT‑‑ATCTGCAGTAAAAAAAAGATGCCGATCCGGCGGTAGAGCAGCTCGATAGAGTACTCGGCTCCGAACCGCGCggactcagga                                                                           >  5:439698/1‑142 (MQ=12)
                                                                             cGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATATGGTACTTGATATTGCGGTAGTAGCGGATGG‑‑AAT‑‑ATCTGCAGGAAATTAAAGGTCCCGATGCGTAGGTAGCGCAGGGGGATAGAGTGATCGGCTACGCACCGAGCGGGCGGAGGtt                                                                          >  5:1583356/1‑151 (MQ=16)
                                                                              gg‑TCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAGAATAAGtttt                                                                                                                                       >  2:212065/1‑88 (MQ=255)
                                                                               gctcggcgcgtctaaTCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTc                                                                         <  1:2025353/138‑1 (MQ=255)
                                                                                gccGGAGGTTCGAATCCTCCCGGATGCACCATATCTTCCTAGATATGCCTTTAGTAGGGGTGTC‑‑AAT‑‑ATCAGCAGTAAAATAAGGGTCCCGATGCTTCAGTAGAACAGGGGGTTGGAGTGCTCGGCTCCGCGCCGCGCGGTGGGAGGTTCg                                                                        >  3:1438338/3‑151 (MQ=9)
                                                                                        gtataaTCCTCCCGGAGTCACCATCTCTTACTTGATATGGCTTTACTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAACTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAAtcctcc                                                                <  1:171985/147‑1 (MQ=255)
                                                                                          ataaccctccCGGATCCACCATCTCTTACTAGATATGTCTTTACTAGCTGTATA‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCg                                                              <  5:1718345/146‑1 (MQ=14)
                                                                                          ataaTACTCCCGGATGCACGATCTCTTTCTTTATATGGCTTTATTAGCGCTATC‑‑AAT‑‑ATCTGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCg                                                              <  5:635808/149‑1 (MQ=14)
                                                                                             atgctgacGGGTGCTCTATCTCTATCTTTATCTGTCTTTACTAGGGCTATA‑‑AAT‑‑ATCAGCAGTAAACTAACTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGAt                                                           <  3:524941/144‑1 (MQ=11)
                                                                                             aTCCTCGCGGTAGCACGCTCTCTTACTTGAGCTGTCTTTTGTCGGGTTCTC‑‑AAT‑‑ATCAGCAGTAAACTAATTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGGCGGAGGTTCGAATCCTCCCGGAt                                                           <  5:1040112/151‑1 (MQ=11)
                                                                                                   ccGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCAcc                                                      >  5:1850356/1‑150 (MQ=40)
                                                                                                    cGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGAAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCACCCGGATGCACCat                                                    >  8:1997026/1‑151 (MQ=255)
                                                                                                         ccaagaTCTCTGAATTTATGTCGCTTTATGAGCTGTCTA‑‑ATT‑‑AACAGCAGTCAAATAATATTCGCTGTCCCCACGTAGGTTACCTGGTTAGAGTAATCGGCCACGATGCGCCCGCTCTGTGGTTGGAATCCTCCCGGATGCACCATATtct                                               <  7:943033/146‑1 (MQ=2)
                                                                                                          cccgaTCTCTTTCTTTAGCTCGCTATATTAGCTCTAAA‑‑AAT‑‑ATCAGCAGTAAACTAACTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATtctc                                              <  3:1314087/147‑1 (MQ=11)
                                                                                                          cACCATCTCTTACTTTACTTCGTTTTACTAGCGTTATC‑‑AAT‑‑ATCTGCAGTAAAATAATATTCCCGCTGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCGACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATtctc                                              <  7:1809738/151‑1 (MQ=11)
                                                                                                          cACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATtctc                                              >  8:1190150/1‑151 (MQ=39)
                                                                                                            ccATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCg                                            >  6:1624324/1‑151 (MQ=39)
                                                                                                             cATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑ACA‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCGGTAGCTCAGCTGGAGAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCGTCCCGGCTGCCGCATCTTCTCCgc                                           >  7:772123/1‑150 (MQ=14)
                                                                                                             aaTCTCTTACTTTATATGGCTTTAGTAGGGGTATA‑‑ATT‑‑ATAAGAAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAcc                                                                                         <  8:2019763/104‑1 (MQ=14)
                                                                                                              aTCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCTAATCCTCCCGGATGCACCatataatcaga                                           >  6:317203/1‑143 (MQ=255)
                                                                                                                  ctTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGTAAACt                                      >  1:1309706/1‑151 (MQ=255)
                                                                                                                    tACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTc                                    >  7:1459974/1‑151 (MQ=255)
                                                                                                                     agttGATATGGCTTTAGTAGCGGTATA‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATtct                                               <  6:544210/137‑1 (MQ=255)
                                                                                                                     aCTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATtct                                               >  5:544520/1‑139 (MQ=40)
                                                                                                                       ttGATACGGCTTTAGTAGCGGTATCAAAAA‑‑ATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTACGATATATTc                                    >  2:1651085/1‑150 (MQ=14)
                                                                                                                        tGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTACCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTAGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAAACTTAAGc                                 >  4:1883479/1‑150 (MQ=255)
                                                                                                                        tGATACGGCTTTAGTAGCGGTATCAAAAA‑‑ATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGATCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATAGTCCTAAATATTc                                    >  7:2008102/1‑149 (MQ=14)
                                                                                                                         gATACGGCTTTAGTAGCGGTATCAAAAA‑‑ATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTGTACTAAATATTcctt                                 >  3:2023406/1‑148 (MQ=14)
                                                                                                                          atatGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATACGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATACTCCCGGCTGCACCATATTCTccctaaccagaacgga                               >  8:1353853/1‑136 (MQ=11)
                                                                                                                          atacGGCTTTAGTAGCGGTATCAAAAA‑‑ATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTATCCTAAATATTCAgg                                 >  8:1954773/1‑149 (MQ=14)
                                                                                                                          atacGGCTTTAGTAGCGGTATCAAAAA‑‑ATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATAGTACGAAATATTCatg                                 >  1:1474098/1‑148 (MQ=14)
                                                                                                                           tacggCTTTAGTAGCGGTATCAAAAA‑‑ATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATAATACGAAATATGCAgaa                                >  6:1991235/4‑148 (MQ=14)
                                                                                                                            acggCTTTAGTAGCGGTATCAAAAA‑‑ATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTATACTAAATATTCATCAAt                              >  6:715651/3‑151 (MQ=14)
                                                                                                                               gCTTTAGTAGCGGTATCAAAAA‑‑ATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATACTACTAAATATTCAgatatta                            >  8:1623430/1‑144 (MQ=14)
                                                                                                                                cTTTAGTAGCGGTATCAAAAA‑‑ATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTGTACGATATATTcctgaatgatg                          >  3:1786076/1‑141 (MQ=14)
                                                                                                                                 tttAGTAGCGGTATCAAAAA‑‑ATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATACTCCTAAATCTTCAGCAATGaacg                         >  3:1606512/1‑149 (MQ=11)
                                                                                                                                 tttAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAACCTTCAGCAATGAAGGt                        >  4:176394/1‑150 (MQ=38)
                                                                                                                                  ttAGTAGCGGTATCAAAAA‑‑ATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTACGAAATATTCATCAATGAACGt                        >  4:868577/1‑151 (MQ=11)
                                                                                                                                    agtagCGGTATCAAAAA‑‑ATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGTAATATTCAGCAATTAACGTAt                      >  1:1329909/1‑151 (MQ=11)
                                                                                                                                    agtagCGGTATA‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGTAACCTTCAGCAATGAAGGTATc                     <  2:1311446/150‑1 (MQ=16)
                                                                                                                                      tatcggtataaaaaT‑‑ATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATc                     <  3:867635/138‑1 (MQ=11)
                                                                                                                                      tagCGGTATCAAAAA‑‑ATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTGTCCGAAATCTTCAGCAATGAAGGTAtgg                    >  8:246671/1‑149 (MQ=11)
                                                                                                                                      tagCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGa                   >  3:190787/1‑150 (MQ=255)
                                                                                                                                       agCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGa                   >  8:917566/1‑149 (MQ=255)
                                                                                                                                       agCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTAGGCTACGAAcc                                                                                         >  6:1336392/1‑79 (MQ=255)
                                                                                                                                        gCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGa                   >  7:1115932/1‑148 (MQ=255)
                                                                                                                                        gCGGTATC‑‑AAAAAATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATATTCAGCAATGAAGGGATCGa                   >  4:763185/1‑150 (MQ=11)
                                                                                                                                         aggtatcaaaaT‑‑ATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAag                 <  6:1257791/142‑1 (MQ=11)
                                                                                                                                         agctgtctaaaT‑‑ATCTGCAGTAAATTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGaa                  <  1:1249674/141‑1 (MQ=11)
                                                                                                                                          ggTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGTAACCTTCAGCAATGAAg                          >  7:1375084/1‑139 (MQ=18)
                                                                                                                                          ggTATA‑‑AAT‑‑TTAAGCAGTAAATTATATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGTAACCTTCAGCAATGAAg                          <  2:972238/139‑1 (MQ=11)
                                                                                                                                           ggttaaaaaT‑‑ATCAGCAGTAAAATAACTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAagag               <  3:129181/144‑1 (MQ=255)
                                                                                                                                           gTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAACCTTCAGCAATGAAGGTATCGAag                 >  5:1536399/1‑147 (MQ=38)
                                                                                                                                            tata‑‑AAT‑‑ATCCGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGTAACCTTCAg                                  <  3:1512118/129‑1 (MQ=255)
                                                                                                                                            tata‑‑AAT‑‑ATCAGCACTAATATAAATTACCCGATTGATGATTCTCTCAGGTGGTGAGAGTAGTCGTCTTCGTACCTAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGt             <  3:806883/150‑1 (MQ=14)
                                                                                                                                            tATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGt             >  1:186795/1‑150 (MQ=255)
                                                                                                                                               ataaaT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGTAACCTTCAg                                  <  4:1455336/125‑1 (MQ=255)
                                                                                                                                                tcaaaaaatCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGACATCTTCAGGAATGATGGGATGGAAGAGGtg            >  3:1823230/8‑150 (MQ=11)
                                                                                                                                                 caaaaaatCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTATACTAAATATTCATCAATTAACGTATAGAAGAGGTac           >  6:1767630/7‑150 (MQ=11)
                                                                                                                                                 aaaT‑‑TTCTGCAGTAAATTAATTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATGCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCg         <  4:1609004/150‑1 (MQ=255)
                                                                                                                                                  aaaaaatCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCTAAATCTTCAGGAATGAAGGTATCGAAGAGGTAGc          >  2:279745/6‑151 (MQ=14)
                                                                                                                                                  aaT‑‑ATCAGCAGTAAAATACATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAAtcct                                                                  <  5:556674/93‑1 (MQ=255)
                                                                                                                                                  aaT‑‑ATCACCAGTAAAATAAATTTCCCGCTGCATGAGTCGCGCAGATGGCTAGAGTCCTCGGATACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCgg        <  1:1963852/151‑1 (MQ=14)
                                                                                                                                                    t‑‑atCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCgg                                                                               >  1:938628/1‑78 (MQ=255)
                                                                                                                                                    aaaatCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATATGGCGGAATGAAGGTATGGAAGAGGTAGAgg        >  6:488123/4‑151 (MQ=11)
                                                                                                                                                      aatCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTATCCTAAATATTCAGAAATTAAGGTATCGAatagcaagagta       >  4:1681158/2‑142 (MQ=11)
                                                                                                                                                       atCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATg                                                          >  6:141425/1‑98 (MQ=40)
                                                                                                                                                       atCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAAt     >  4:2014012/1‑151 (MQ=255)
                                                                                                                                                       agcagcagTAAAATATATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATg                                                          <  5:141632/96‑1 (MQ=255)
                                                                                                                                                        tCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTc                                                                         <  2:1985708/82‑1 (MQ=40)
                                                                                                                                                         cagcagTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAAt                                                                     >  2:745344/1‑85 (MQ=39)
                                                                                                                                                         cagcagTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAAt                                                                     <  1:744726/85‑1 (MQ=39)
                                                                                                                                                          agcagTAAAATAACTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTaa  <  7:1736415/151‑1 (MQ=255)
                                                                                                                                                          |                                                                                                                                                      
TCAAAAAATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTAA  >  DH1/1065456‑1065755

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑