Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH11,065,6180AG23.7% 377.7 / 132.5 240intergenic (+53/‑10)ECDH1_RS05245/ECDH1_RS05250tRNA‑Arg/tRNA‑Arg
Reads supporting (aligned to +/- strand):  ref base A (101/60);  new base G (41/9);  total (147/93)
Fisher's exact test for biased strand distribution p-value = 1.50e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.19e-01

Only 100 of 268 total aligned reads displayed.

AGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTAACCGCAGGCACTAG  >  DH1/1065474‑1065768
                                                                                                                                                    |                                                                                                                                                      
gccaaagcgcccTATGCATCCGTAGCTGAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTccc                                                                                                                                                  <  5:441927/142‑1 (MQ=255)
agtaagtTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTATTACTTGATATGGCTTTATTAGCGTAAAC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTacc                                                                                                                                                  >  8:735535/1‑151 (MQ=18)
      ttgcgctctGCATCCGTAGCTCAGCTGTTTAGACTACTCGGCGACTATCGGTTCGGTCTGAGGGTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGc                                                                                                                                             <  8:1142278/142‑1 (MQ=18)
             gagCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGATCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAg                                                                                                                                     <  7:432890/149‑1 (MQ=255)
              tGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAg                                                                                                                                     <  7:1024857/150‑1 (MQ=255)
                         aagagagcgcttccctACTCGGCGACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATa                                                                                                                         <  3:227279/136‑1 (MQ=18)
                                ggtttgtctACTCGGCTACGATCGTGTCGGTCGGCGAGTCTAATCCTCGGGGATGCACGATCTCTTAGTTGATATGGCTTTAGTAGGGTTATC‑‑AAT‑‑ATCTGCAGTAAACTAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACt                                                                                                                  <  4:328692/143‑1 (MQ=9)
                                 gATTGAGTACTCGGCTACGATCGCGGCGGTCGGAGGGTCTAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTc                                                                                                                 <  7:1774015/151‑1 (MQ=17)
                                          cTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATGTTACTTGATATGGCTTTAGGATCGGTAGG‑‑AAG‑‑ATCTGCAGTAATTTAACTTTGCCGATTCGTCAGTAGCTCAGGGGGATTGACTACTCGGATCCGac                                                                                                        >  7:606349/1‑150 (MQ=12)
                                            gggctctgagcgcgtcGGTCGGAGGGTCTAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAcc                                                                                                      <  3:128244/136‑1 (MQ=14)
                                            cGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATGGTACTTTATATTCATGAAGGAGCGTTAGC‑‑AAT‑‑ATGAGCAGTAAATTACATTTCCCTATACATACGAAGCAGATCGGGATTGAGTCGTCGGCTGCGAGcc                                                                                                      >  1:1051343/1‑151 (MQ=9)
                                             ggctacttacataGCGTTCGGAGGTTAGAATCATCCAGTATTCACTATCTCTTATTTCATATCTCGTTAGTATCGGTATC‑‑TAA‑‑ATCTGCAGTAAATTAACTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCg                                                                                                     <  8:316312/139‑1 (MQ=9)
                                                 acctatctgccGGTCGGCGCGTCTAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCg                                                                                                 <  7:826903/141‑1 (MQ=14)
                                                    agcgcgccgctCGGAGCGTCGAATCCTCCGTGATGCAACATCTCTTACTTGATATGGCTTTAGTAGCGGTATA‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTc                                                                                              <  1:30736/141‑1 (MQ=14)
                                                     aCCGAGCTCTCGGAGCGTCTTATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTACTAGCTGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTc                                                                                              <  5:338741/150‑1 (MQ=14)
                                                        gAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTAGG‑‑AAG‑‑ATCACCAGTAATAAAACGATGCAGATTCATCAGTAGCGCAGCTGGATATAGTCCTCGGAAAAGACCCGCgcgggcggcg                                                                                          >  7:58329/1‑149 (MQ=12)
                                                          gCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTTGTACTTGATATGCCTGTAGTATCGTTTGC‑‑AAT‑‑ATCTGCAGGAAATTAACGGTCCCGATGGATAAGAAGCACAGGGGGTTAGAGTGGGCGGCTGGGCGCCGCGCGCTCGGAgtt                                                                                        >  7:1679137/1‑149 (MQ=9)
                                                            ggTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAGAATAAGtttt                                                                                                                                                    >  2:212065/1‑88 (MQ=255)
                                                                ggcgcgtataaTCCTCGGTGATGCACGATCTCTTAGTTGATATGGCTTTAGTAGCGCTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAAt                                                                                  <  5:1556002/142‑1 (MQ=11)
                                                                     ttCGAATCCTCCCGGATGCACCATCTGTTACTTGATATGGCTTTAGTAGCGTTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATACATACGTAGAGCAGCGGGATAGAGTACTCGGATACGAACCGACGGGACGGAGGTTCGAAtcatcc                                                                             >  3:1644062/1‑151 (MQ=14)
                                                                      tctaaTCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTATTAGCGCTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAAtcctcc                                                                             <  1:475898/147‑1 (MQ=255)
                                                                      tCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTTTC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTccc                                                                            >  5:549175/1‑151 (MQ=255)
                                                                      tCGAATCCTCCCGGATGCACCATATAATACTTAATATTCATAAAGTAACGGAAAC‑‑AAT‑‑ATCTACAGTAAAAAAAATAACCCAATACATCATAAGAACAGCTGCATATAGTAAAAGACTACGAACATAACGGTAGCAGGATCGAATCCTccc                                                                            >  6:376513/1‑151 (MQ=255)
                                                                       attaTCCTCCGCGATGCACCATCTCTTACTTTATCTGGCTTTAGTAGCGTTATC‑‑AAT‑‑ATCAGCAGTAAACTAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTccc                                                                            <  6:543567/147‑1 (MQ=14)
                                                                         tattctcgcgttttcACCATCTCTTTCTTGATTTGGCTTTTGTAGCTTTATA‑‑AAT‑‑ATCAGCAGTAAATTAATTTTCCCTATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCgg                                                                          <  4:901097/136‑1 (MQ=11)
                                                                              tccCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCAc                                                                    >  6:1024334/1‑151 (MQ=39)
                                                                                  ggATGCACCATCTCTTAATTGATATCGCTTTAGTATCGTTATC‑‑AAT‑‑ATCAGAATTAAAATAAATTTACCTATGCATCCGTAGCTCAGCTTGATAAATAAATCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCat                                                                 >  6:1451886/1‑150 (MQ=11)
                                                                                     tGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTACCGATGCATCCGTAGCTCAGCGGGATAGAGTACTAGGCTACGAACCGACCGGTCGGAGGTTACAATACTGCCCGCTGCACCATAtt                                                              >  3:2005781/1‑150 (MQ=14)
                                                                                      gCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATtct                                                            >  1:213386/1‑151 (MQ=39)
                                                                                      gCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATtct                                                            >  1:1782589/1‑151 (MQ=39)
                                                                                       cccgaTCTCTTTCTTTAGCTCGCTATATTAGCTCTAAA‑‑AAT‑‑ATCAGCAGTAAACTAACTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATtctc                                                           <  3:1314087/147‑1 (MQ=11)
                                                                                       cACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATtctc                                                           >  8:1190150/1‑151 (MQ=39)
                                                                                       cACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATtctc                                                           >  4:1989565/1‑151 (MQ=39)
                                                                                         ccATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTACCGATGCATACGTAGCAAAGCTGGATAGAGTACTAGGCTACGAACCGAGCGGTCGGAGGTTAGAATCCTCACGGATGCaaaaacttatacc                                                         >  8:19958/1‑139 (MQ=14)
                                                                                          aaTCTCTTACTTTATATGGCTTTAGTAGGGGTATA‑‑ATT‑‑ATAAGAAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAcc                                                                                                      <  8:2019763/104‑1 (MQ=14)
                                                                                              tctTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTAGGCTACGAACCGAGCGGTCGAAGGTTCGAATCATACCGGAAGCACAATATTCTACGAAAt                                                     >  6:978404/1‑150 (MQ=14)
                                                                                               tccgttgtttatGGCTTTGGTAGCGTTATC‑‑ATT‑‑ATCAGAAGTAAAATACATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGtaacc                                                    <  7:1353876/141‑3 (MQ=11)
                                                                                               ctTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGTAAACt                                                   >  1:1309706/1‑151 (MQ=255)
                                                                                                 tACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTc                                                 >  7:1459974/1‑151 (MQ=255)
                                                                                                    ttGATACGGCTTTAGTAGCGGTATCAAAAA‑‑ATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTACGATATATTc                                                 >  2:1651085/1‑150 (MQ=14)
                                                                                                     tGATACGGCTTTAGTAGCGGTATCAAAAA‑‑ATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGATCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATAGTCCTAAATATTc                                                 >  7:2008102/1‑149 (MQ=14)
                                                                                                      gATACGGCTTTAGTAGCGGTATCAAAAA‑‑ATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAGATATTCAgg                                              >  1:1180861/1‑150 (MQ=14)
                                                                                                       atttGGGTTTATTATGGGTATG‑‑AAT‑‑ATCTTCTGTAAAATAATTTTCCCGTTGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCaa                                            <  8:462940/147‑1 (MQ=11)
                                                                                                       atacGGCTTTAGTAGCGGTATCAAAAA‑‑ATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATAGTACGAAATATTCatg                                              >  1:1474098/1‑148 (MQ=14)
                                                                                                       atacGGCTTTAGTAGCGGTATCAAAAA‑‑ATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATACTACGAAAtatgccgc                                              >  2:221471/1‑143 (MQ=14)
                                                                                                        tatGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATg                                          >  3:596246/1‑151 (MQ=255)
                                                                                                        tacggCTTTAGTAGCGGTATCAAAAA‑‑ATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATAATACGAAATATGCAgaa                                             >  6:1991235/4‑148 (MQ=14)
                                                                                                             cTTTAGTAGCGGTATCAAAAA‑‑ATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTGTACGATATATTcctgaatgatg                                       >  3:1786076/1‑141 (MQ=14)
                                                                                                              tttAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGt                                     >  8:2071767/1‑150 (MQ=255)
                                                                                                              tttAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAACCTTCAGCAATGAAGGt                                     >  4:176394/1‑150 (MQ=38)
                                                                                                                   tatcggtataaaaaT‑‑ATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATc                                  <  3:867635/138‑1 (MQ=11)
                                                                                                                   tagCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGTAAACTTCAGCaa                                            >  1:1705952/1‑138 (MQ=25)
                                                                                                                   tagCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGa                                >  3:190787/1‑150 (MQ=255)
                                                                                                                    agCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGa                                >  8:917566/1‑149 (MQ=255)
                                                                                                                    agCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGa                                >  4:1057033/1‑149 (MQ=255)
                                                                                                                    agCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGATGGATAGAGTACTCGGATACGAACCGAGCCGTCCGACCTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGCTATCGaat                              >  8:1815944/1‑150 (MQ=14)
                                                                                                                     gCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGa                                >  7:1115932/1‑148 (MQ=255)
                                                                                                                     gCGGTATC‑‑AAAAAATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATATTCAGCAATGAAGGGATCGa                                >  4:763185/1‑150 (MQ=11)
                                                                                                                       gctATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCGGTAGATCTGCTGGATAGAGTACTGTGCTACGAACCGAGCTGGCGCAGGTTGGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAgg                           <  7:93535/149‑1 (MQ=14)
                                                                                                                        ttata‑‑AAT‑‑ATCTGCAGTAAACTAATTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAgg                           <  8:795399/149‑1 (MQ=255)
                                                                                                                        ggaTC‑‑AAT‑‑ATCATCACTAAAATAAATTTACCGCTGCATCCTTACCTCCCCTGGATATACGACTGGTCTACTAACCATGTGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGt                          <  5:2006717/149‑1 (MQ=14)
                                                                                                                         tata‑‑AAT‑‑ATCCGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGTAACCTTCAg                                               <  3:1512118/129‑1 (MQ=255)
                                                                                                                         tATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGt                          >  1:186795/1‑150 (MQ=255)
                                                                                                                         tATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTACCTATGAATCCGTAGCTCAGCTGAATAAAGTAATCGAATACGAAAAGAGCGGAAGGAGGTTCGAATCCTAAAAGAAACACCATATTCTCAGAAATCATCAGCAATCAACGAATCCAAGAgga                          >  6:1280686/1‑149 (MQ=255)
                                                                                                                          aTC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGCGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGt                          >  5:1713786/1‑149 (MQ=255)
                                                                                                                           gc‑‑AAT‑‑ATCAGCAGTAAATTAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTAc                                                                                                           <  3:1614870/66‑1 (MQ=255)
                                                                                                                            attaaT‑‑ATCTTCAGTAAATTAATTTTCCCGATGCATCCGTAGGTCAGCTGGATAGAGTACTCGGCTAGGAAGCGATGTGTCTTAGGTTCTAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTa                         <  6:235743/147‑1 (MQ=14)
                                                                                                                              caaaaaatCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTATACTAAATATTCATCAATTAACGTATAGAAGAGGTac                        >  6:1767630/7‑150 (MQ=11)
                                                                                                                               aaT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAg                                                                                                                                >  3:1362495/1‑44 (MQ=255)
                                                                                                                                at‑‑atCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCg                                                                                                                <  1:140819/59‑1 (MQ=255)
                                                                                                                                 t‑‑atCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCgg                                                                                            >  1:938628/1‑78 (MQ=255)
                                                                                                                                   aatCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTATCCTAAATATTCAGAAATTAAGGTATCGAatagcaagagta                    >  4:1681158/2‑142 (MQ=11)
                                                                                                                                    atCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATg                                                                       >  6:141425/1‑98 (MQ=40)
                                                                                                                                    agcagcagTAAAATATATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATg                                                                       <  5:141632/96‑1 (MQ=255)
                                                                                                                                     tCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTc                                                                                      >  1:1983053/1‑82 (MQ=40)
                                                                                                                                     tCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTc                                                                                      <  2:1985708/82‑1 (MQ=40)
                                                                                                                                      ctgcagTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTATACTAAATCTTGAGATATTAAGGTATACAagatctacagtaatt                 >  4:1355863/3‑138 (MQ=11)
                                                                                                                                       aggattAAAGTAAATTTCCCTCTGCATCGGTAGGTCAGCTGGATAGATTAATCGGCGAGGATGCGTTGGGTCCTAGGTTGTAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTaa               <  7:749778/146‑1 (MQ=9)
                                                                                                                                        gcagTAAACTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTa                <  5:1190832/149‑1 (MQ=255)
                                                                                                                                        gcagTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTGCTCGGCTACGAACCAAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCAGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGGGGAATTAAc              >  6:2064930/1‑151 (MQ=255)
                                                                                                                                        gcagTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGTAACCTTCAGCaa                                            <  2:1823192/121‑1 (MQ=18)
                                                                                                                                         cagTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTGTCCTAGATCTTCAGCTATTAAGGTATAGTAGAGGTAGAGGAATTAAcc             >  2:837749/1‑151 (MQ=14)
                                                                                                                                         cagTAAAATAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTAAcc             <  5:1768760/151‑1 (MQ=255)
                                                                                                                                         cagTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGTAAACTTCAGCAATGAAGGTATCGAAGAGATAGCGGTATTAAcc             >  4:835319/1‑151 (MQ=9)
                                                                                                                                          agTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTAACCg            >  5:1367172/1‑151 (MQ=255)
                                                                                                                                           gTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTAACCGc           <  7:1906233/151‑1 (MQ=255)
                                                                                                                                             aaagTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTTATACGAGATCTGCAGCAATTAAGGGTTAGAAGAGGTAGAGGAATTAAcagaac         >  2:1042768/1‑146 (MQ=14)
                                                                                                                                              aaataaatTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAAtcctcc                                                                             <  3:534983/82‑1 (MQ=37)
                                                                                                                                              aaataaatTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAAtcctcc                                                                             >  4:536404/1‑82 (MQ=37)
                                                                                                                                               aataaatTTCCCGATGCATCCGTAGCTCAGCTGGATa                                                                                                                         >  2:994676/1‑37 (MQ=255)
                                                                                                                                               aataaatTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTAACCGCAGGc       >  2:1350070/1‑151 (MQ=255)
                                                                                                                                               aataaatTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAAACTTCAGCAATGAAGGTATCGAAGAGATAGCAGatctgaagagagtc       >  4:2030716/1‑138 (MQ=11)
                                                                                                                                                ataaatTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTAc                                                                                                           <  2:799130/50‑1 (MQ=38)
                                                                                                                                                ataaatTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTAc                                                                                                           >  1:798662/1‑50 (MQ=38)
                                                                                                                                                ataaatTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATAtt                                                              >  8:1735475/1‑95 (MQ=35)
                                                                                                                                                 taagtTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTAACCGCAGGCa      <  4:1536589/150‑1 (MQ=255)
                                                                                                                                                  aaatTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAAACTTCAGCAATCAAGGTATCGAAGAGATAGCGGAATTAACAGCAGCCAc     >  8:1459312/1‑150 (MQ=255)
                                                                                                                                                   aatTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTa                                                                                                                    >  5:591650/1‑38 (MQ=35)
                                                                                                                                                   aatTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTa                                                                                                                    <  6:591460/38‑1 (MQ=35)
                                                                                                                                                   aatTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTAACCGCAGGCACt    >  7:896027/1‑150 (MQ=40)
                                                                                                                                                    |                                                                                                                                                      
AGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTAACCGCAGGCACTAG  >  DH1/1065474‑1065768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑