Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH12,169,6730AT25.9% 140.8 / 35.2 161R629R (CGT→CGA) rsxCelectron transporter RsxC
Reads supporting (aligned to +/- strand):  ref base A (47/50);  new base T (0/34);  total (62/99)
Fisher's exact test for biased strand distribution p-value = 1.40e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 161 total aligned reads displayed.

TCTGGCTCCGCATTAGCCGGTTGCTGTTCCCGTTTGCGCGCTTTGGCTCGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGC‑TCGGCATTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCTGTTCTTCTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTGCGCGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCTGTTGTTCTGG  >  DH1/2169530‑2169822
                                                                                                                                                |                                                                                                                                                     
tctGGCTCCGCATTAGCCGGTTGCTGGTCCCGTTTGCGCGCTTTGGCTCGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGC‑TCGGCATTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCACGGGCa                                                                                                                                                 <  7:1453948/150‑1 (MQ=255)
 caggcgcctcaagaGCCGGCTGCTGGTCCCGTTTGCTCGCGTGCGCGCGGGCAATAGCGGCTGCGACTGCCGCTTTGCGCGGATCCACCTGTTGTTCTGGC‑TCGGCATTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCACGGGCAAt                                                                                                                                               <  5:1111392/138‑1 (MQ=17)
   tcctcgggtttAGCCTTTTGCTGTGCCGGTTTGTTGGCCTTTGCGCGGGCAATAGCGGCTTCTACGGCCGCTTTGCGCGGATCGACCTGTTGTTCTGGC‑TCGGCATTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCACGGGCAATa                                                                                                                                              <  4:371047/141‑1 (MQ=11)
      tacGCATGAGCAGGTGGCGGTTCCAGATTGCGGGCTGTGCCTGGCGCTATAGCGGCTTCGCCTGCGTCTTTGCGCGGATCTACCTGTTGTTCTGGC‑TCGGCATTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCACGGGCAATAGCg                                                                                                                                           <  1:1461487/148‑1 (MQ=14)
       ccGCATTAGCCGGCTGCTGTGCCCGGTTGCGCGCCTGGGCGCGGGCAATAGCGGCTTCGACTGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGC‑TCGGCATTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCACGGGCAATAGCGGc                                                                                                                                         <  7:1638155/151‑1 (MQ=14)
        cccagtcgccggccgCTGTGCCCGGTTGCGCGCGTTGGCTCGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGC‑TCGGCATTAGCCTGGTGCTGTTCCAGCTTGCGTGCTTTGGCACGGGCAATAGCGGCt                                                                                                                                        <  7:1476604/144‑1 (MQ=14)
         gccatcgcggggtGCTGTTCCCTCTTGTTCGCTTGTGCGCGGGCAATAGCGGCTTCTACTGCCGCTTTGCGCGGATCTACCTGTTGTTCTGGC‑TCGGCATTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCACGGGCAATAGCGGCtt                                                                                                                                       <  8:1301079/139‑1 (MQ=14)
         gCATGATCCGGGTGCTGTTCCCGGTTTCGGGCGTTGGCGCGGGCAATAGCCGCGTCGACGGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGC‑TCGGCATTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCACGGGCAATAGCGGCtt                                                                                                                                       <  7:1230921/151‑1 (MQ=14)
          gatgatccGGGTGCTGGGCCGGTTTGGGGGCGTTCGCGCGGGCAATAGCCGCTGCGACGGCCGCTTTGCGCGGATCGACCTGTTGTTCTGGC‑TCGGCATTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCACGGGCAATAGCGGCTTc                                                                                                                                      <  1:1186263/145‑1 (MQ=14)
          cATTAGCCTTTTGCTGGTCGGGTTTTTGGGCTTTTGCGCGGGCAATAGCGGCTTCTACTGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGC‑TCGGCATTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCACGGGCAATAGCGGCTTc                                                                                                                                      <  8:764358/151‑1 (MQ=14)
          cATTAGCCTGTTGCTGTTCGCGTTTGCGCGCTTTGGCTCGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGC‑TCGGCATTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCACGGGCAATAGCGGCTTc                                                                                                                                      <  4:676491/151‑1 (MQ=255)
           atgaGCCGGGTGCTGGTCCCGTTTGCGGGCGTGGGCGCGGGCAATAGCCGCGTCGACTGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGC‑TCGGCATTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCACGGGCAATAGCGGCTTCg                                                                                                                                     <  7:1243993/148‑1 (MQ=14)
           aTTAGCCGGTTGCTGTTCCCGTTTGCGCGCTTTGGCTCGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGG‑TCGGGATTATGCTGTTGGTGTTCCAGCTTGCGTGGGTTTGCCCGGTCAATAGCttcttct                                                                                                                                     >  7:400922/1‑150 (MQ=14)
           aTTAGCCGGTTGCTGTTCCCGTTTGCGCGCTTTGGCTCGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGA‑TCGGCATTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCACGGGCAATAGAGGCTTCg                                                                                                                                     >  8:468987/1‑151 (MQ=255)
             caGCCGGGTGCTGTTCCGGTCTGGTCGCGTGGGCGCGGGCAATAGCGGCGTCGACGGCCGCTTTGCGCGGATCTACCTGTTCTTCTGGC‑TCGGCATTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCACGGGCAATAGCGGCTTCGAc                                                                                                                                   <  1:1322950/150‑1 (MQ=14)
              acccGGGTGCTGGGCGCGTTTGCGCGCGTGCGCTCGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCTACCTGTTGTTCTGGC‑TCGGCATTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCACGGGCAATAGCGGCTTCGAcg                                                                                                                                  <  7:1096263/149‑1 (MQ=14)
              aGCCGGTTGCTGTTCCCGTTTGCGCGCTTTGGCTCGGGCAGAACCGGATACGACGGCGGGTTTCGGCGGAAAGAGATGTAGTTCTGGG‑TAGGCCTTAGCCTGTTGCTGTTAAAGCTTGCGGGCTTTGGCGCGGGCagggggggcgtggccg                                                                                                                                  >  7:628744/1‑136 (MQ=255)
              aGCCGGGTGCGGGGCCCGTTTGGGCGCGTGCGCGCGGGCAATAGCCGCGTCGACTGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGC‑TCGGCATTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCACGGGCAATAGCGGCTTCGAc                                                                                                                                   <  3:1313878/150‑1 (MQ=14)
              aGCCGGATGCTGGTCGCGTTTGCGGGCGTGCGCGCGGGCAATAGCCGCGTCGACGGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGC‑TCGGCATTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCACGGGCAATAGCGGCTTCGAcg                                                                                                                                  <  5:1969655/151‑1 (MQ=14)
                gcttttGCTTTTTCCGCTTGCGGTCTTTGTTGCGTGCTATAGCCGCTTCTACTGCCGCTTTGCGCGGATCTACCTGTTGTTCTGGC‑TCGGCATTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCACGGGCAATAGCGGCTTCGAcgg                                                                                                                                 <  4:1427540/146‑1 (MQ=14)
                cgtgTTGCTGTGGCCCGTTGCGGGCGTGGGCGCGGGCAATAGCCGCTTCTACGGCCGCTTTGCGCGGATCGACCTGTTGTTCTGGC‑TCGGCATTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCACGGGCAATAGCGGCTTCGAcggc                                                                                                                                <  1:1831572/148‑1 (MQ=14)
                cctggtGCTGTTCGCGGTTTCGCGCTTTTGCGCGGGCAATAGCGGCGTCTACTGCCGCTTTGCGCGGATCGACCTGTTGTTCTGGC‑TCGGCATTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCACGGGCAATAGCGGCTTCGAcggc                                                                                                                                <  5:428214/146‑1 (MQ=14)
                  ggTTGCTGTTCCTGTTTGCGATATTTTGATCTGGCTATAGCTGCTTCGAGGGCTTCTTTGCGCGGATCTACCTGTTGTTCTGGC‑TCGGCATTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCTCGGGCAATAGCGGCTTCGAcggcgg                                                                                                                              <  8:1097062/151‑1 (MQ=14)
                    gtGCTGTTCCCGTTTGCGCGCTTTGGCTCGGGCAATAGCGGCTGCGACGGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGC‑TCGGCATTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCt                                                                                                                            <  3:1366389/150‑1 (MQ=255)
                    gtGCTGGTCCCGTTTGCGCGCGTTGGCGCGGGCAATAGCGGCTGCGACGGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGC‑TCGGCATTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCACGGGTAATAGCGGCTTCGACGGCGGCt                                                                                                                            <  7:1136822/150‑1 (MQ=255)
                      gctgggcgggTTTGCGGGCGTGGGCGCGGGCAATAGCCGCTTCCACGGCCGCTTTGCGCGGATCGACCTGTTGTTCTGGC‑TCGGCATTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCttt                                                                                                                          <  5:1475124/142‑1 (MQ=14)
                      gCTGTTCGTGTTTTCTGGCTTGAGCTCGGGCAATAGCGGCTTCTACTGCTGCTTTGCGCGGATCTACCTGTTCTTCTGGC‑TCGGCATTAGCCTGTTGCTGTTCCAGATTGCGTGCTTTGGCTCGGGCAATAGCGGCTTCGACGGCGGCttt                                                                                                                          <  6:283212/151‑1 (MQ=11)
                      gCTGTTCCCGTTTGCGCGCGTTGGCGCGGGCAATAGCGGCTTCTACGGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGC‑TCGGCATTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCttt                                                                                                                          <  5:587578/151‑1 (MQ=255)
                       cggctCCCGATTGCGCGCTTTGGCTCGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGC‑TCGGCATTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTg                                                                                                                         <  7:115781/147‑1 (MQ=255)
                        ggtccccGCTTGCGAGCTTTCGCGCGGGCAATAGCCGCGTCGAGGGCTGCATGGCGCGGATCGACCGGCGGTTCGGCC‑TCCGCACTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTgc                                                                                                                        <  1:436386/147‑1 (MQ=11)
                         gttcgtgtttgttggcTTTGGCTCGTGCAATAGCGGCTTCTACGGCTGCTTTGCGCGGATCTACCTGTTGTTCTGGG‑TCCGCATTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCTCGTGCAATAGCGGCTTCGACGGCGGCTTTgcg                                                                                                                       <  4:1998129/137‑1 (MQ=11)
                          gagccGTTTGCGGGCGTGCGCGCGGGCAATAGCGGCTGCTACTGCGGCTTTGCGCGGATCCACCTGTTGTTCTGGC‑TCGGCATTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTgcgg                                                                                                                      <  5:467182/148‑2 (MQ=14)
                             cggttttggggcgttGGCGCGGGCAATAGCCGCTTCGACTGCCGCTTTGCGCGGATCGACCTGTTGTTCTGGC‑TCCGCATTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGa                                                                                                                   <  3:2008728/138‑1 (MQ=11)
                             ccGCTTTCGCGCGTGTGCTCGGGCAATAGCCGCGGCCACTGCCGCTTTGCGCGGATCAACCTGTTCTTCTTGC‑TCGGCATTAGCCTGTTGCTGTTCCCGGTTGCGTGCTTTTGCTCGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGa                                                                                                                   <  8:1241241/151‑1 (MQ=11)
                               cgttGCGCGCCTGCGCTCGGGCAATAGCGGCGTCGACGGCCGCGTTGCGCGGATCCACCTGTTGTTCTGGC‑TCCGCATTAGCCTGCTGCTGTTCCAGCTTGCGTGCTTTGGCCCGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATc                                                                                                                 <  1:244172/149‑1 (MQ=14)
                                cgtGCGCGCTTTGGCTCGTTCAATAGCCGCTTCGACGGCGGCTTTGCGCGGATCGACCTGGTCTTCTGGC‑TCGTCTTTAGCGTGTTGCTGTTCCAGCTTGCGTGCTTTGGCTCGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCg                                                                                                                <  7:251115/149‑1 (MQ=14)
                                 ttGCGCGCTTTGGCTCGGGCAATAGCTGCTTCGACGGCAGGATGGCAAGGAGAGACGTCTTCTT‑TGCCGTCACAATAAGAATGGTGCTGTTCCAGCTTGAGCGGTATTGAAAGCGCTATAGCGGCTTCGAcggcggggtgggcgggggcgg                                                                                                               >  5:1895283/1‑136 (MQ=255)
                                   gggcgcggtGGCGCGCGCAATCGCCGCGGCGTCGGCGGCTTTGCGCGGATCGACCTGTTCTTCTGGC‑TCGGCATTAGCCTGTTGCTGTTCCAGCTTGCGGGCGTTGGCTCGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGAcc                                                                                                             <  4:629154/143‑1 (MQ=11)
                                   ccccgcTTTGGCGCGGGCAATCGCCGCTGCGTCGGCCGCTTTGCGCGGATCTACCTGTTGTTCTGGC‑TCGGCATTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGAcc                                                                                                             <  2:1391280/148‑1 (MQ=14)
                                      ggccttGGCGCGGGCAATAGCCGCGGCCACGGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGC‑TCGGCATTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGAcc                                                                                                             <  1:868538/144‑1 (MQ=14)
                                        cTTTGGCTCGGGCAATAGCGGCTTCGACGGCGGGTTTGCGCGGATCGCCCTGTTGTTCTGCG‑TCGGGATTAGCCTGTTGGTGTTCCAGCTTGCGTGCTTTGGCCCGGTTAATCGCTTCTTCGCCGGCACTTTTTCGAGGATCATCCTggcg                                                                                                        >  3:1236425/1‑148 (MQ=2)
                                           gagatCGTGCAATAGCTGCTGCCTCTTCCGCTTTGCGCGGATCTACCTGTTGTTATGGC‑TCGGCATTAGCCTGTTGCTGTTCGTGTTTGCGTGCTTTGGCTCGGGCAATAGCGGCTTCTACTGCGGCTTTGCGCGGATCGACCTGttctt                                                                                                      <  8:1176332/146‑1 (MQ=11)
                                            ggcccgttcactaccGGCTTCGACCGCCGCTGAGCGCGTATCGACCTGTTCTTCTGGC‑TCGGCATTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCTGTtcttct                                                                                                    <  8:445069/137‑1 (MQ=11)
                                            gccTCGGGCAGGAGCGGCTACGACGTCCGCTTCCCCCGTATCAACCTGTTCTTCTGGC‑TCCGCATTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCTGTtcttct                                                                                                    <  3:465378/149‑1 (MQ=11)
                                            cgcgcGGGCAATAGCGGCGGCGACTTCCGCGTTGCGCGGATCGACCTGTTGTTCTGCT‑TACGCATTAGCGTGTTGCTTTTCCAGCTTGCGTGCTGTGGCGCGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCTGTtcttct                                                                                                    <  3:966291/147‑1 (MQ=11)
                                             gggcGGGCAATAGCGGCGGCCAGTGGGTCTTTGGGCGGTTGGACCTGGTGTTCTGGC‑TGGGGTTTAGCCTGTTGCTGTTCCAGGTTGTGTGCTTGTGCTCGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCTGTTCTTCTg                                                                                                   <  2:1992315/148‑1 (MQ=9)
                                             gCTCGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATAGAGCTGATGATCTTCG‑TCGTCATTAACATGTTGATGTTAAATCTTGCTAGCTTTGGAAGGCGGATTATATGAATAGACGGCGGCTTTGGGCGGATGGagcgggtgggcgg                                                                                                   >  8:1812600/1‑139 (MQ=2)
                                                  ggCAATAGCCGCGTCAACTGCGGCTTTGCGCGGATAGACCTGTTGTTCTGGC‑TCGGCATTAGCCTGTTGCTGTGCCAGCTTGCGTGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCTGTTCTTCTGGTTcc                                                                                              <  5:49797/151‑1 (MQ=14)
                                                    cAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGG‑TCGGCATTAGCCTGTTGCTGTTCCAGCTTGCGGGCTTTGGCACGGGCGATAGCGGCTTCCACGGCGGATTTTCGTGGATCGGCATCTGGTTCTGGGTcctt                                                                                            >  3:245402/1‑149 (MQ=11)
                                                      aTAGCCGCCTCGACGGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGC‑TCGGCATTAGCCTGTTGCTGTTCCAGCTTGCGTACTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCTGTTCTTCTGGTTCCGCAt                                                                                          <  8:803126/151‑1 (MQ=255)
                                                        aGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGC‑TCGGCATTAGCCTGTTGCTGTTCCAGCTTGCGAGCTTTGGCACGGGCAATAGCTGCTTCAACGGCTGATTTACGAGGATCGCCCTCTGCTTCTGCTTcctaattt                                                                                        >  8:1842681/1‑145 (MQ=11)
                                                         tcgtattCGAATGCGGCTTTGCGCTGATCTACCTTTTGTTCATGA‑TCCTTATTATCCTGTTGGTTGTTCAGATCTTTTGCTTTCGCGCGGGCAATAGCCGCTTCTACTGCCGCTTTGCGCGGATCGACCTGTTCTTCTGGTTCCGCATTa                                                                                        <  6:1390794/145‑1 (MQ=255)
                                                          cGGCTTCGACGGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGC‑TCGGCATTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCCCGGGCAATAGCTGCTTCGACGGCTGGTTTTCGAGGATCGCCCTGTTGTGCTGCGTCCggagttgg                                                                                      >  1:382916/1‑144 (MQ=11)
                                                              ttCGACGGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGG‑TCGGCATTAGCCTGTTGCTGTTCCAGATTGCGTGCTTTGGCCCGGGCGATAGCGGCTTCTACGGCGGATTTACGCGGATCATACTCATCTCCTGCTTCCGCATTactgac                                                                                   >  5:1176983/1‑145 (MQ=14)
                                                               tCGACGGCGTCTTTGCGGTGATCGAGCTGTTGTTCTGGT‑TCGGCATTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCTCGTGAAATAGCGTCTTCGACGGCGTCTTTGAGCGGATCGAGCTGTTCTTCTGGTTCCGCATTAGCCt                                                                                    <  8:453533/148‑1 (MQ=11)
                                                               gcGACTGCGGCTTTGCGCTGATCGACATGTGGTGCTCGG‑TGGGCAGTAGCCTTCTGCTGGGCGAGCTTGCTGGCTTTCGCTCGGGCAATAGCGGCTTCTACTGCGGCTTTGCGCGGATCGACCTGTTCTTCTGGTTCCGCATTAGCCTg                                                                                   <  7:1614236/148‑1 (MQ=2)
                                                                  tcttccgcTTTTCGAGGATCTTCCTTTTTTTCTGGC‑TCGGCATTATCCTTTTGCTGTTCGTGTTTTTTTGCGTTTGCTCGGGCAATAGCCGCTTCTACTGCGGCTTTGCGCGGATCGACCTGTTCTTCTGGTTCCGCATTAGCCTGTTg                                                                                <  4:1654926/143‑1 (MQ=9)
                                                                      cggcTTTGCGCGGATCGACCTGTTGTTCTGGC‑TCGGCATTAGCCTGTTGCTGTTCCAGCTTGCGAGCTTTGGCACGGGCAATAGCTGCTTCGACGGCGGATTTACGAGGATCAACAAGTTGTTCTGCTTCCGGATTATGCTGTTGGTCTTc                                                                          >  2:639908/1‑151 (MQ=11)
                                                                       ggcTTTGCGCGGATCGACCTGTTGTTCTGGC‑TCGGCATTAGCCTGTTGCTGTTCCAGCTTGCGAGCTTTGGCACGGGCAATAGCTGCTTCAACGGCAGATTTACGAGGATCAACAACTTTTTCTGCTTCCTAATTATCCTgaagatcttc                                                                          >  6:372873/1‑141 (MQ=1)
                                                                        gcTTTGCGCGGATCGACCTGTTGTTCTGGC‑TCGGCATTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCTCTTCTTCTGGTTCCGCATTAGCCTGTTGCTGTTcc                                                                         >  1:1736569/1‑150 (MQ=255)
                                                                         cTTTGCGCGGATCGACCTGTTGTTCTGGC‑TCGGCATTAGCCTGTTGCTGTTCCAGCTTGCGAGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGGTTTGCGCGGATCGCCAACTGGTGCTGGGTCCTCATTATCCTGATGGTCTTcc                                                                         >  7:641062/1‑149 (MQ=11)
                                                                              cgcgGATCGACCTGTTGTTCTGGC‑TCGGCATTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCACGGGCAATAGCGGCTTCTACGGCGGCTTTCCGGGGATCGGCCTGGTGTTCTGGGTCGTGAGTATCCTGTTGGTGTTCCTGATTgc                                                                  >  1:320583/1‑151 (MQ=11)
                                                                               gcgGATCCAACTGTTCTTCTGTC‑TATTCATTAGACTGTTGCTGTTCAAGATTGTGTTCTTTTGTTCGGGCAATAGCGGCTTCTACTGCGGCTTTGCGCGGATCTACCTGTTCTTCTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTgcg                                                                 <  8:82906/151‑1 (MQ=11)
                                                                               gcgGAGCCACCTGTTGTTCTGGC‑TCCGCATTAGCCTGTTGCGGTTCCTGGTTGGGTGCGTTGGCTGGTGCAATCGGCGCTTCGACGGCCGCTTTGCGCGGATCGACCTGTTCTTCTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTgcg                                                                 <  7:1803409/151‑1 (MQ=11)
                                                                                 ggATCGACCTGTTGTTCTGGC‑TCGGCATTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACATGTTGTTCTGGTTCCTCATTAGCCTGTTGCTGTTCCAGCTTgcgcg                                                               >  3:1694211/1‑151 (MQ=255)
                                                                                        agtcgagttCTTGC‑TCGTCATTAGCCTGGTGCTGGTCGTGCTTTCTGGCCTTCGCGCGGGCAATAGCCGCTGCCACTGCCGCTTTGCGCGGATCCACCTGTTCTTCTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTGCGCGCTTTGGc                                                        <  3:1787863/145‑1 (MQ=2)
                                                                                         cTGTTGTTCTGGC‑TCGGCATTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCACGGGCTATAGCTGCTTCGACGGCCGGTTTGCGGGGATCGGCAACGGTTGCTGCTTCCGTATTTTCCTGTTGATGTTCCAGtttcggagtggatggc                                                       >  1:207868/1‑138 (MQ=2)
                                                                                          tgttgtTCTGGC‑TCGGCATTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCACGGGCTATAGCGGCTTCAACGGCCGATTTACGGGGATCGGCATCTTTTTCTGATAGCGGATTAGCCTCATTCTGATCGAGCTTccgagtcatcgctc                                                      >  3:815323/1‑140 (MQ=9)
                                                                                             tgttCTGGC‑TCGGCATTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCCCGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGCCATGGTGTTCTGGGTCGGGATTTGGCTGTTGCTGTTCCAGGTTGCGCGTTTTGGccgggg                                                   >  7:1917364/1‑146 (MQ=11)
                                                                                              tttCGGGC‑TCGGTATGACCCTTGTGCTGGGCGTGTTTGCTGGCCTTTGCGCGGGCAATAGCGGCTGCTACTGCCGCTTTGCGCGGATCAACCTGTTCTTCTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTGCGCGCTTTGGCACggg                                                   <  8:1282276/149‑1 (MQ=2)
                                                                                                 cTGGC‑TCGGCATTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCGCGGGCAATAGCGGCTTCGACGGCGGGTTTGCGTGGATCGGCATGGTAGAGGGGTTGCGCAGTAGGCTGATCGGGTGGCAGCTTGAGAGCTGTGGCACggggtat                                               >  5:865647/1‑147 (MQ=2)
                                                                                                 cTGGC‑TCGGCATTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTCCGCGGATCGCCCAGTTGTGCTGGTTCCGCATTGGACTGTTGGTGTTGCAGCTGGCCGGCTGTGGCGCGGGCTAt                                               >  7:773436/1‑151 (MQ=11)
                                                                                                 cTGGC‑TCGGCATTAGCCTGTTGCTGTTCCAGCTTGCGAGCTTTGGCACGGGCAATAGCGGCTTCAACGGCAGATTTACGAGGATCAGCCACGTATGCTGCTTCCGGATTATCCTGATGATCTTCCAGATTCAGCTCTTAGGAACgtccaa                                                >  6:2040422/1‑145 (MQ=2)
                                                                                                    gC‑TCGGCATTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTGCGCGCTTTGGCACGGGCAATAGc                                            >  7:442815/1‑151 (MQ=37)
                                                                                                     gctcgtgtttAGCCTGTTGCTGTTCGTGTTTTCTTGCGTTAGCTCGGGCAATAGCCGCGTCGACTGCGGCTTTGCGCGGATCTACCTGTTCTTCTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTGCGCGCTTTGGCACGGGCAATAGc                                            <  6:447705/143‑1 (MQ=14)
                                                                                                     gctcgtgaatAGCCTGGTGCTGTGCGCGGTTGTTGGCCTTCGCTCGGGCAATAGCGGCTGCTACTGCCGCTTTGCGCGGATCTACCTGTTCTTCTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTGCGCGCTTTGGCACGGGCATTAGc                                            <  3:147941/142‑1 (MQ=2)
                                                                                                            aTTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCTGTGGTTCTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTGCGCGCTTTGGCCCGGGCAATAGCTGCTTCg                                     >  1:1092136/1‑151 (MQ=2)
                                                                                                              tAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCGCGGGCAATAGCGGATTCGCCGGCGGCTTTGCTCGAGTCACAGTGTGCTTGTGGGTAGGCAGTAGGCTGGTGGTGTTAAAGGTTGCGGGCGTTGGCGCGGGcgggcgcgggtgcgcg                                   >  5:698175/1‑136 (MQ=2)
                                                                                                                 ccTGGTGCTGTGCCCGGTTTCTGGCCTTTGCGCGGGCAATAGCCGCGGCTACGGCGGCTTTGCGCGGATCTACCTGTTCTTCTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTGCGGGCTTTGGCACGGGCAATAGCGGCTTCGAcggc                                <  6:230650/151‑1 (MQ=11)
                                                                                                                  cTGTTGCTGTTCCAGCTTGCGTGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGGTTTGCGAGGATCGGCCTGTTGTGCTGGTTCCGGATTTTCCTGATGGTCTTCCAGGTTGCGAGGGGTGGCCCGGGCAATAGATGCTTCCAcggcg                               >  3:1643862/1‑151 (MQ=2)
                                                                                                                   tGTTGCTGTTCCAGCTTGCGTGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCTGTTCTTCTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTGCGCGCTTTGGCCCGGGCAATAGCTGCTTCGAcggcgg                              >  5:943629/1‑151 (MQ=255)
                                                                                                                      tgctgcTCCAGCGGGCGTGCTTTCGGTCGGGCTCTCGCGGCTGCGACGTCGTGTTCGCTCGGATGCTCCTGTTCTTCTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTGCGCGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCt                            <  7:932176/150‑1 (MQ=14)
                                                                                                                        cTGTTCCAGCTTGCGTGCTTTGGCTCGGGCAATAGCGGCTTCGACGGCTGCTTTGCGCGGATCGACCTGTTCTTCTGGTTCCGCATTAGCCTGTTGCTATTcc                                                                         <  5:1621220/103‑1 (MQ=255)
                                                                                                                         tGTTCCAGCTTGCGTGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGGTTTGCGCGGATCGACCTGTTCTTCTGGTTCCGCATTAGCCTGTTGGTGTTCCAGCTTGCGCGCTTTGGCCCGGGGAATAGCTGCTTCGACGGCTGGttttc                        >  7:1512652/1‑149 (MQ=11)
                                                                                                                          gggcgggttttcgggCTTTTGCGCGGGCAATAGCGGCGTCTACTGCCGCTTTGCGCGGATCAACCTGTTCTTCTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTGCGCGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTgc                        <  2:485381/136‑1 (MQ=11)
                                                                                                                          gTTCCAGCTTGCGTGCTTTGGCTCGTGCAATAGCGGCTTCGACGGCTGCTTTGCGCGGATCGACCTGTTCTTCTGGTTCCGCATTAGCCTGtt                                                                                 <  2:40003/93‑1 (MQ=255)
                                                                                                                           ttCCAGCTTGCGTGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGCCCTGTTGTTCTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTGCGCGGTTTGGCCCGGGCCATAGCGGCTTCTACGGCAGTTTTgcgc                      >  3:391434/1‑151 (MQ=11)
                                                                                                                            gcgggttttcGTGCGTTAGCTCGGGCAATAGCCGCTGCTACTGCCGCTTTGCGCGGATCTACCTGTTCTTCTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTGCGGGCTTTGGCTCGGGCAATAGCGGCTTCGACGGCGGCTTTGcgcg                     <  2:577581/142‑1 (MQ=11)
                                                                                                                             ccAGCTTGCTGGCCTGCGCGCGGGCAATAGACGCTGCTACGGCGGCTTTGCGCGGATCTACATGTTGTTCTGGTTCTGCATTAGCCTGTTGCTGTTCCAGCTTGAGTGCTTTGGCTCGGGCAATAGCGGCTTCGACTGCGGCTTTGCGCgg                    <  6:1766398/151‑1 (MQ=11)
                                                                                                                             ccAGCTTGCGTGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGCCCTGTTCTTCTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTGCGCGATTTGGCACGGGCAATAGCTGCTTCGACGGCGGGTTTACGAgg                    >  5:1653893/1‑151 (MQ=14)
                                                                                                                              cAGCTTGCGTGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCTGTTCTTCTGGCTCCGCATTAGCCTGTTGCTGTTCCAGCTTGCGCGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGa                   <  7:469019/151‑1 (MQ=255)
                                                                                                                               tgcgtgcttgctTTCGCGTGGGCAATAGCGGCTTCTACTTCGGCTTTGCGCGGATCTACCTGTTCTTCTGGTTCGGCATTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCTCGGGCAATAGCGGCTTCGACGGCTGCTTTGCGCgg                    <  6:1446426/145‑1 (MQ=11)
                                                                                                                                gCTTGCGTGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCTGTTATTCTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTGCGAGATTTGGCACGGGAAATAGCTGCTTCTACTGCAGATTTgcgacgat                  >  2:703976/1‑145 (MQ=11)
                                                                                                                                ccgcgcgcgcggcggCCCGTGCTATAGCCGCCTCCACGGCGGCTAGGCGCGTTCGGACCTTTTCTGCAGGTCCGGCCTTAGCGGGTCCGTGCTCCAGCTTGCGCGCTTTGGCCCGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATc                 <  3:1387071/138‑1 (MQ=2)
                                                                                                                                  ttGCGTGCTTTGGCACGGGCAATAGCTGCTTCAACGGCAGGTTAGATCGGAACAGCCTCGTGTTCTGAATCAGAATTAACATTTTTCTTTTCCAGCTTGCTCGCATTAGCAATATAAATAGCTGCTTCGACGGCGGGTTTGCGCggctggg               >  8:2005357/1‑146 (MQ=255)
                                                                                                                                   tGCGTGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGCCATGTTGTTCTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTGCGCGCTAGGGCTCGGGCCATAGCAGCTTCACCGCCCGCTTTTCGCGGATcctc              >  5:321945/1‑148 (MQ=11)
                                                                                                                                    gCGTGCTTTGGCGCGTGCAATAGCGGCTTCGACGGCTGCTTTGCGCGGATCGACCTGTTCTTCTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTGCGCGCTTTGGCACg                                                     <  4:1037745/111‑1 (MQ=255)
                                                                                                                                         cTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCTGTTCTTCTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTGCGCGCTTTGGCACGGGCAATAGCTGCTTCTACGGCAGGTTTTCGAGGATcatcatgggg        >  1:1015101/1‑142 (MQ=11)
                                                                                                                                          tttGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCTGTTCTTCTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTGCGCGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGGTTTGCGAGGATCGACCtggtgt       >  5:245253/1‑151 (MQ=255)
                                                                                                                                               cACGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCTGTTCTTCTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTGCGCGCTTTGGCGCGGGCAATAGCGGCTTCGACGGCTGGTTTGCGCGGATCTACCTCGTGTTCTgg  >  5:564845/1‑151 (MQ=14)
                                                                                                                                                |                                                                                                                                                     
TCTGGCTCCGCATTAGCCGGTTGCTGTTCCCGTTTGCGCGCTTTGGCTCGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGC‑TCGGCATTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCTGTTCTTCTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTGCGCGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCTGTTGTTCTGG  >  DH1/2169530‑2169822

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑