BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	DH1	2,326,287	0	G	T	29.9%	972.9 / 171.0	544	intergenic (+207/+200)	yddM/fdnI	transcriptional regulator/formate dehydrogenase nitrate‑inducible cytochrome b556(Fdn) subunit
Reads supporting (aligned to +/- strand): ref base G (247/126); new base T (35/124); total (288/256)
Fisher's exact test for biased strand distribution p-value = 3.32e-21
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 551 total aligned reads displayed.

GATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGA‑CGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATG  >  DH1/2326137‑2326437

  tGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATAAAAATGA‑AGCCTTTTACACCGCCATTTGa                                                                                                                                                       >  7:1575403/1‑150 (MQ=16)

   gcgcTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGTATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGA‑CGCCTTTTACAGCGCCATTTGaaa                                                                                                                                                     <  4:1464653/151‑1 (MQ=21)

   gcgcTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGA‑CGCCTTTTACAGCGCCATTTGaaa                                                                                                                                                     <  2:1971412/151‑1 (MQ=34)

     gcTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGA‑CGCCTTTTACAGCGCCATTTGAAACt                                                                                                                                                   <  7:1026566/151‑1 (MQ=35)

      cTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGA‑CGCCTTTTACAGCGCCATTTTAAACtt                                                                                                                                                  >  6:134542/1‑150 (MQ=18)

         cGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGA‑CGCCTTTTACAGCGCCATTTGAAACtgatg                                                                                                                                               <  3:1264930/151‑1 (MQ=39)

          gCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGA‑CGCCTTTTACAGCGCCATTTGAAACtgatga                                                                                                                                              <  3:679232/151‑1 (MQ=39)

                 aGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGA‑CGCCTTTTACAGGCGCAATAGAAACTGATGCCAAACg                                                                                                                                        >  1:1769356/1‑150 (MQ=11)

                    ccTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGA‑CGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAAc                                                                                                                                    <  1:1862487/151‑1 (MQ=255)

                       actagTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGA‑CGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACAt                                                                                                                                  <  8:1346337/146‑1 (MQ=255)

                       acacGTTCACTTCAATCTATTGGATTTGAAGGGTTTTCATGCCTTATAATCCGTTCACGCCGCATCCGGCATGAATAAAGCCTACTTTTTCTACAATATTAAAATGA‑CTCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACa                                                                                                                                   <  6:1795599/149‑1 (MQ=14)

                       acacGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGA‑CGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACa                                                                                                                                   <  8:1459870/149‑1 (MQ=255)

                          cGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGTATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGA‑CGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGcc                                                                                                                              <  8:1027071/151‑1 (MQ=255)

                          cGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGA‑CGCCTTTTACAGCGCCATTTTAAACTGATAAAAAAATAAACAttac                                                                                                                               >  2:1034767/1‑148 (MQ=11)

                           gTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGA‑CGCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGACt                                                                                                                             >  4:1414494/1‑151 (MQ=255)

                           gTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCATTAACGAAGCATCAGGAATGAACAAAGACTAATTTGTATACAATATGAAAATGA‑CGACTTATACAGATAAATTTAAAAATGATGACAAACGCAACATTGCCt                                                                                                                             >  4:327122/1‑151 (MQ=14)

                            ttCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGA‑CGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTg                                                                                                                            >  7:1849722/1‑151 (MQ=255)

                              cACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGA‑CGCCTTTTACAGCGCCATTTGAAACTGATGAGAAAATCAACATTGAATGAt                                                                                                                          >  5:2032708/1‑151 (MQ=255)

                                 tGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGA‑CGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATgcg                                                                                                                       >  7:1674629/1‑151 (MQ=255)

                                 tGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGA‑CGCCTTTTACAGCGCCATTAGAAACAGATGACAAACGCAACATAGACTGATgcg                                                                                                                       >  8:1407404/1‑151 (MQ=255)

                                  gCAATCTATTGGATTTGAAGGGTTTTCATGCCGTATAAGGCGTTCACGCCGCATCCGTCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGA‑CGCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATgc                                                                                                                        <  8:734832/149‑1 (MQ=255)

                                  gCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGA‑CGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATgcg                                                                                                                       <  3:1587562/150‑1 (MQ=255)

                                   cAATCTATTGTATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGA‑CGCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCt                                                                                                                     <  5:1305570/151‑1 (MQ=255)

                                   cAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGA‑CGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCt                                                                                                                     >  1:1983099/1‑151 (MQ=255)

                                   cAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGA‑CGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGAAACATTGCCTGATGCGCt                                                                                                                     >  5:851427/1‑151 (MQ=255)

                                    aaTCTATTGGATTTGAAGGGTTTTCATGCCGGATAACGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGA‑CGCCTATTACAGCGCCATTTTAAACTTATAACAAAAAAAACAttaaatcaatccca                                                                                                                     >  2:255538/1‑138 (MQ=11)

                                        tATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGA‑CGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCt                                                                                                                >  4:1253733/1‑151 (MQ=255)

                                           tGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGA‑CGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTAt                                                                                                             >  1:247894/1‑151 (MQ=255)

                                            gtctttCAAGGGTTTTCATTGCGTATAAGTGTTTCACGCCGCATCGTGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGA‑CGCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTAt                                                                                                             <  2:582322/147‑1 (MQ=18)

                                              aTTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGA‑CGCCTTTTACAGCGCCATTTTAAACTGATGACAAAAGCAACATTGCCTGATGCGCTACGCTTATc                                                                                                            >  2:1759461/1‑149 (MQ=255)

                                              aTTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGA‑CGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATc                                                                                                            >  1:86247/1‑149 (MQ=255)

                                              aTTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGA‑CGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATc                                                                                                            >  5:1816649/1‑149 (MQ=255)

                                                ttGAAGGGTGTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGA‑CGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAgg                                                                                                         <  1:1362365/150‑1 (MQ=255)

                                                 tGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGA‑CGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGcc                                                                                                       >  3:508161/1‑151 (MQ=255)

                                                 tGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGA‑CGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGAGCTACGCTTATCAGGcc                                                                                                       >  6:360870/1‑151 (MQ=255)

                                                   tatggTTTTGATCCCGGATATGTCGATCACTCTTCATACTGATTGAATAAAGGCTTCGTTTTCTACAATATGAAAATGT‑CTCTTTATAAAGCGCCATTTTTAACTGATGACAAACGCAACATTGCCTGATGCGATACGCTTATCAGGCCt                                                                                                      <  6:1891265/147‑1 (MQ=2)

                                                   aaGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGA‑CGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCt                                                                                                      >  1:1216504/1‑150 (MQ=255)

                                                   aaGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGA‑CGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCt                                                                                                      >  2:482756/1‑150 (MQ=255)

                                                      tgTTTTCTTTCCTGATTAGGCGTTCACGACGCATCTGGCATGAATAAAGCCTTCGTTTTATACAATATTAAAATGA‑CTCCTTTTAAAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTaca                                                                                                   <  2:1591636/149‑1 (MQ=18)

                                                       gTTTTCATGCCGGATATTTTTTTAACTCAGCAGCCGTCATGAAAAATGTATACTTTTTTTAAAATATTTTAATTA‑CTCCTTTTTCTTCGGATTTTTTAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACg                                                                                                 <  4:1098618/151‑1 (MQ=2)

                                                       gTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGA‑CGCCTTTTACAGCGCCATTTGAAACTGATCACAAAAGCAACATTCACTCATGCGCTACGCTTATCAGGCCTACACg                                                                                                 >  6:884836/1‑151 (MQ=255)

                                                          ttCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGA‑CGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTc                                                                                              >  1:1529670/1‑151 (MQ=255)

                                                               gacgtaTAAGGCGTTCACTCCTCATCCTGCATGAACAAAGCCTACGTTGTCTACAATATTAAAATGA‑CGCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGc                                                                                         <  6:1583124/146‑1 (MQ=25)

                                                               gCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGA‑CGCCTTTTACAGCGCCATTTGAAACTGATGACACACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGc                                                                                         >  7:921257/1‑151 (MQ=255)

                                                                ccGGATAAGCCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGA‑CGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCCGGCCTACACGTTCACTGCa                                                                                        <  5:1008866/151‑1 (MQ=255)

                                                                    attagcgtttCACGAGGGATCCGGGATGAAGAAAGCCTACGTTGTCTTCAATATGAAAATGA‑CTCCTTTTAAAGCGCCATTTTTAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCt                                                                                    <  2:1213304/143‑1 (MQ=17)

                                                                    aTAAGTCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGA‑CGCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCt                                                                                    <  1:604627/151‑1 (MQ=255)

                                                                    aTAAGGCGTTCACGCCGCAACCGGCATGAACAAAGCCTACTTTGTATACAATATCAAAATGA‑CGCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCt                                                                                    <  5:2021044/151‑1 (MQ=255)

                                                                     tacggCTTTAACGCCGGATCCGGCATGAACAAAGCCTACGTTGTCTACAATATTAAAATGA‑CGCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCt                                                                                    <  6:1123162/147‑1 (MQ=21)

                                                                      aaGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGA‑CGCCTTTTACAGCGCCATTTTAAACTCAAGAAAAACCCAACATTACCTAATGAGATAAACTTATAAGGACTAAAACTTAACTGCAAtata                                                                                   >  6:1654621/1‑150 (MQ=14)

                                                                      aaGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGA‑CGCCTTTTACAGCGCCATTTGAAACTGATGAGAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTAt                                                                                  >  2:360550/1‑151 (MQ=255)

                                                                       aGGCGTTCACCACGCATCCTGCATGAACAAAGCCTTCGGTGTATACAATATGAAAATGA‑CGCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTAtt                                                                                 <  5:1092944/151‑1 (MQ=21)

                                                                        ggCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGA‑CGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTg                                                                                <  7:571397/151‑1 (MQ=255)

                                                                         gCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGA‑CGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCGACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTgg                                                                               >  5:1901656/1‑151 (MQ=255)

                                                                           gTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGA‑CGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGAt                                                                             >  2:792001/1‑151 (MQ=255)

                                                                            ttaaCGCCGAATACGGAATTAAAAAATACTATTTTGTCTATAATATTAAAATTAGCGCTTTTTA‑TGTGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGa                                                                              <  4:1971535/146‑1 (MQ=2)

                                                                            ttCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGA‑CGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCGTATCAGGCCTACACGTTCACTGCAATCTATTGGAtt                                                                            >  3:953120/1‑151 (MQ=255)

                                                                                   cgcATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGA‑CGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAggg                                                                     >  1:402285/1‑151 (MQ=255)

                                                                                   cgcATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGA‑CGCCTTTTACAGCGCCATTTGAAACTGATGACAAAAGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAggg                                                                     >  8:1206101/1‑151 (MQ=255)

                                                                                    gcATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGA‑CGCCTTTTACAGCGCCATTTGAAACTTATAACAAACTCAACATTAACTGATGATATAATATTATAAGTCATACACGTTCACTGCAATCTATTAGAATTGAAGGGt                                                                    >  2:1364380/1‑151 (MQ=18)

                                                                                      aTCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGA‑CGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGttt                                                                  >  5:1596366/1‑151 (MQ=255)

                                                                                           gtaTGAATTAAGCCTTCTTTGTATACAATATTAAAATGT‑CTCTTTTTACAGCGCCATTTTTAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCAt                                                              <  6:1455500/148‑1 (MQ=14)

                                                                                           gCATGAAAAAAGCCTTCTGTGTATACAATATTAAAATGA‑CTCTTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATg                                                             <  8:1906600/151‑1 (MQ=14)

                                                                                            cATGAACAAAGCCTACGTTGTCTACAATATGAAAATGA‑CGCCTTTTACAGCGCCATTTGAATCTGATGACAAACAATACATTGCCTGATGCGCTCAGCTTATCAGGACTACACGTACACTGCAATCTATTGGATTTGAAGCTTTTTCaatc                                                            >  1:1074948/1‑148 (MQ=21)

                                                                                                 aatattaatACTTTTTCTAGAATATTATATTTA‑CGTATTTTAAAGCGCCATTTTTAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGAt                                                       <  8:1880762/143‑1 (MQ=11)

                                                                                                 aCAAAGCCTACGTTGTCTACAATATGAAAATGA‑CGCCTTTTACAGCGCCATTTTAAACTGATGACAAACGAAACATTGCCTGATGCGCTACGCTTATCAGACATACACGTTCACTGCAATCTATTGGATTTGAAGGTTTTTCATGCCGGAt                                                       >  4:1616029/1‑151 (MQ=255)

                                                                                                 aCAAAGCCTACGTTGTCTACAATATGAAAATGA‑CGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGAt                                                       >  3:355073/1‑151 (MQ=255)

                                                                                                   taagacttctttttatACAATATTAAAATGA‑CTCATTTTAAAGCGCCATTTTTAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATa                                                      <  4:768112/135‑1 (MQ=11)

                                                                                                   aaaGCCTACGTTGTCTACAATATGAAAATGA‑CCCCTTTTAAAGAGCCATTTGACCATGAAGACAACCGCAACATTGCATGCTGCTATAAGCTTCTAAGGCCTACACTGTCACTGCAATCTAATGGATTTGAAGTGTTTTAATTCCGGATa                                                      >  5:416831/1‑150 (MQ=19)

                                                                                                   aaaGCCTACGTAGTCTACAATATAAAAATGC‑CCCCTTTTAAAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATaa                                                     <  1:130942/151‑1 (MQ=14)

                                                                                                     aGCCTACGTTGTCTACAATATGAAAATGA‑CGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGTCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATTCCGGATAAgg                                                   >  2:1533991/1‑151 (MQ=255)

                                                                                                     aGCCTACGTTGTCTACAATATGAAAATGA‑CGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAgg                                                   >  3:1161555/1‑151 (MQ=255)

                                                                                                      gCCTACGTTGTCTACAATATGAAAATGA‑CGCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGc                                                  <  7:1502890/151‑1 (MQ=255)

                                                                                                         tACGTTGTCTACAATATGAAAATGA‑CGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGtt                                               >  8:1569195/1‑151 (MQ=255)

                                                                                                         aaagTTTTCTACAATATAAAAATGA‑CGCCTTATACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGtt                                               <  5:1206759/148‑1 (MQ=255)

                                                                                                          aCGTTGTCTACAATATGAAAATGA‑CGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGCATTTGAAGGGTTTTCATGCCGGATAAGGCGTTc                                              >  6:491879/1‑151 (MQ=255)

                                                                                                            gTTGTCTACAATATGAAAATGA‑CGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCAc                                            >  2:1827792/1‑151 (MQ=255)

                                                                                                            gTTGTATTCAATATTAAAATGA‑CTCCTTTTAAAGCGCCATTTTTAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCAc                                            <  8:312154/151‑1 (MQ=11)

                                                                                                              tGTCTAGAATATGTAACTTC‑CGAGTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCAcg                                           <  3:243861/150‑1 (MQ=14)

                                                                                                              tGTCTACAATATGAAAATGA‑CGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCAcgc                                          >  5:1451505/1‑151 (MQ=255)

                                                                                                              tGTCTACAATATGAAAATGA‑CGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCAcgc                                          >  7:1393525/1‑151 (MQ=255)

                                                                                                              tGTCTACAATATGAAAATGA‑CGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCAcg                                           <  3:1363909/150‑1 (MQ=255)

                                                                                                                tattcAATATTAAAATGA‑CTCCTTTTACAGCGCCATTTTTAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCAcgcc                                         <  4:577711/146‑1 (MQ=14)

                                                                                                                  tACAATATGAAAATGA‑CGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCAcgccgc                                       >  5:305416/1‑150 (MQ=255)

                                                                                                                    aaaTATGAAAATGA‑CGCCTTTTACTGCGCCATTTGAAACTGATGCCAAACTCAAGATGGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATc                                    <  1:778140/150‑1 (MQ=255)

                                                                                                                     aaTATGAAAATGA‑CGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATcc                                   >  7:284872/1‑151 (MQ=255)

                                                                                                                        atGAAAATGA‑CGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGc                                >  7:2073744/1‑151 (MQ=255)

                                                                                                                         tGAAAATGA‑CGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGc                                >  4:479017/1‑150 (MQ=255)

                                                                                                                          gAAAATGA‑CGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCAt                              >  5:593398/1‑151 (MQ=255)

                                                                                                                             aaTGA‑CGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGa                            >  4:171824/1‑150 (MQ=255)

                                                                                                                                gA‑CGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACa                         >  3:1866459/1‑150 (MQ=255)

                                                                                                                                     ccTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGcc                    >  5:526254/1‑151 (MQ=39)

                                                                                                                                        tatACAGCGCCATGTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGAGTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTa                  <  1:1639042/148‑1 (MQ=19)

                                                                                                                                         ttACAGCGCCATTTTTAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTAc                 <  2:95077/150‑1 (MQ=17)

                                                                                                                                          tACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCAGGCATGAACAAAGCCTACGt               >  6:720458/1‑151 (MQ=25)

                                                                                                                                           aCAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGtt              >  7:390195/1‑151 (MQ=35)

                                                                                                                                                 ccATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTAc        <  1:470095/151‑1 (MQ=14)

                                                                                                                                                 ccATTTGAAACTGATTACAAACTCAACATTGCCTTATGGGTTACGCTTTTCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTAc        <  8:49932/151‑1 (MQ=11)

                                                                                                                                                 ccATTTGAAACTGATAACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTa         >  6:1057338/1‑150 (MQ=18)

                                                                                                                                                     ttGATACTGATGACAACCGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTTCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAat     <  3:1433588/150‑1 (MQ=9)

GATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGA‑CGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATG  >  DH1/2326137‑2326437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`