Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH12,591,5130TG27.1% 269.1 / 59.1 297D86A (GAC→GCC) ECDH1_RS12815hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (114/64);  new base G (21/45);  total (163/134)
Fisher's exact test for biased strand distribution p-value = 1.05e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 299 total aligned reads displayed.

GACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAACGAATCCGCAATCAAATATTCTGCCCAAGCGGGGAAGGATG  >  DH1/2591363‑2591660
                                                                                                                                                      |                                                                                                                                                    
    gtatgcgcgaacgccgcAGCGCGGACTAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTC‑AGc                                                                                                                                                 <  7:1825924/137‑1 (MQ=17)
                    cAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTGGAGCGGC‑AGCGAGAGAGCGTTGCCGc                                                                                                                                 >  3:1553909/1‑151 (MQ=255)
                     aGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACCAAGGTTCGCCTTGAGCGCC‑AGCgag                                                                                                                                              >  3:769159/1‑137 (MQ=255)
                            tggaaTCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTTCAATCGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTC‑AGCGAGAGAGCGTTGc                                                                                                                                    <  4:1253676/137‑1 (MQ=255)
                            aGTAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTC‑AGCGAGAGAGCGTTGCCGCAGGCAACg                                                                                                                         <  6:1598479/151‑1 (MQ=255)
                              tAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACGAAGGTTCGGCTGGAGCGCC‑AGCGAGAGAGCGTTGCCGCAGGCAACGAc                                                                                                                       >  7:969527/1‑151 (MQ=255)
                                  ccTTCCCCCACCACCATCACTTTCAAAACTAAGGGAAAGCTCTAGCTAATCCGGAATCAAGTATTCTCACAATTCTGGGAACGATGAGAAGAGTCGACTAACGTTGGCCTGCATCGGC‑ATCGAAACACCGTTGCCGCCGGcgccgccgcgg                                                                                                                   >  5:1951803/1‑141 (MQ=2)
                                   cTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGa                                                                                                                   >  3:1137702/1‑150 (MQ=255)
                                     tCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAg                                                                                                                 <  6:623710/150‑1 (MQ=255)
                                      cccccACCACCATCACTTTCAAAAGTCCCTGAACTTTCAACCGAATCCGAAATAAAATATTCTGCCCATGCGGGGAAGGATGATAAGCTTCGACTAAGGTTCGGCTCGAGCGTC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAggg                                                                                                               <  8:1410747/151‑1 (MQ=255)
                                      cccccACCACCATCACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAggg                                                                                                               <  5:1596525/151‑1 (MQ=255)
                                       ccccACCACCATCACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACGAAGGTTCGACTAGAGCGAC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGc                                                                                                              >  4:1395820/1‑151 (MQ=255)
                                       ccccACCACCATCACTTTCAAAAGTCCCTGAACTCCCCACCGAATCCGCAATAAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGc                                                                                                              <  3:17874/151‑1 (MQ=255)
                                        cccACCACCATCACTTTCAAAAGTAACGGAACTCTCAAGCGAATCCTCAATCAAATATTCTTCCCAAGCGCGGAAGTATGAGAAGCTTCGACTAAAGTTCGACTCGAGCGGC‑AGCGAGAGAGCGTTGCCGCAGGCCACGCCCCGAAGGGCg                                                                                                             >  1:1020075/1‑151 (MQ=11)
                                          caccaccaTCACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGACTCGAGCGTC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGa                                                                                                            >  8:169588/1‑150 (MQ=255)
                                           cccaccaccaCTTTCATCCGTCCTTTAACTCCCAACCTAATCCGAAATAAAATTTTCTGCCCATGGGGGTAAGGATGATAAGCTTCGACTATGGTTCGGCTCGAGCGCC‑AGCTAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAg                                                                                                          <  6:448143/150‑1 (MQ=11)
                                           accaccaTCACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGTGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGTCTCGAGCGTC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGa                                                                                                            >  8:1604133/1‑149 (MQ=255)
                                           accaccaTCACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGCCTGGAGCGGC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGa                                                                                                            >  3:968306/1‑149 (MQ=255)
                                            ccaccaTCACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACAAAGGTTCGCCTAGAGCGAC‑AGCGAGAGAGCGTTGCCTCAGGCAACGACCCGAAGGGCGAAgc                                                                                                         >  4:552215/1‑151 (MQ=255)
                                             caccaTCACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAgcg                                                                                                        >  1:1873562/1‑151 (MQ=255)
                                             aaccaTCACTTTCAAAAGTCCCTGAACTTACAATCGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAgcg                                                                                                        <  6:1514464/150‑1 (MQ=255)
                                              accaTCACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGagctccgaagagc                                                                                                       >  1:922530/1‑139 (MQ=255)
                                                  ccACTTTCAAACGCCCCGCAACTCCCACGCCAACCCGCAAGAAAATATGCTGCCCATGCGGGGATGGATGAGAAGCTTCGACTACGGTGCGGCGCGCGCGGC‑AGCGAGAGAGCGTGCCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAg                                                                                                   <  5:1799416/150‑1 (MQ=9)
                                                   cACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGTCTCGAGCGTC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAgc                                                                                                  <  4:1848511/151‑1 (MQ=255)
                                                    aCTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAgcg                                                                                                 >  4:926906/1‑151 (MQ=255)
                                                    aCTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGCCTAGAGCGGC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAgcg                                                                                                 >  7:356024/1‑151 (MQ=255)
                                                    aCTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACAAAGGTTCGACTCGAGCGTC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAgcg                                                                                                 >  4:385081/1‑151 (MQ=255)
                                                     cTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACGAAGGTTCGCCTGGAGCGAC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAgcgc                                                                                                       >  2:1795678/1‑144 (MQ=255)
                                                       ttcaaaccgcccTGACCTCACAAGCGAATCCGCATGCAAATATGCGGCCCCGGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCt                                                                                               <  3:903340/141‑1 (MQ=14)
                                                       ttCAAAAGTCCCTGAAGTTTCAATCGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCt                                                                                               <  2:1040085/150‑1 (MQ=255)
                                                       ttCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACAAAGGTTCGACTAGAGCGAC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCt                                                                                               >  6:301577/1‑150 (MQ=255)
                                                       atcacacgtaccTGAACGCTCATGCAAATCCGCAGTGACATCTTCGGCCCTTGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGGC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAgcgc                                                                                                <  5:217433/139‑1 (MQ=14)
                                                        tCAAACGGCCCTCAACTCTCAACCGAACCCGCAATCAAATATTCTGCCCCTGCGGGGAAGGATGAGAAGCTTCGACCAAGGTTCGACTCGAGCGCC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTg                                                                                              <  3:384429/150‑1 (MQ=11)
                                                         cAACAGTCCCTGAACTCCCCACCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGGC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAg                                                                                            <  7:1697236/151‑1 (MQ=255)
                                                          atacgctcttcctatctaAAGCTAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACCAAGGTTCGACTTGAGCGCC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGt                                                                                           <  2:220889/137‑1 (MQ=11)
                                                           aaaGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGt                                                                                           >  7:1005179/1‑150 (MQ=255)
                                                            aaGTCCCTGAACTCTCAATCGAATCCGCAATAAAATATTCTGCCCATGCGGTGAAGGATGAGAAGCTTCGACTAAGGTTCGTCTCGAGCGGC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTaa                                                                                         <  2:1470075/151‑1 (MQ=255)
                                                             aGTCCCTGAACTGTCAAGCGAATCCGCAATAAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGGC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAAt                                                                                        <  2:593458/151‑1 (MQ=255)
                                                              gTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATc                                                                                       >  4:234011/1‑151 (MQ=255)
                                                                 ccTGAACTCTCAAGCTAATCCTCACTCAAATATTCTGACCATGCGTTGAAGGATGAGAATCTTCGACTACGGTTCGGCTCGAGCGTC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCtt                                                                                    <  6:1680178/151‑1 (MQ=17)
                                                                 ccTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACGAAGGTTCGCCTTGAGCGGC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGAGCtcggaagagca                                                                                     >  5:865429/1‑140 (MQ=11)
                                                                   tGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGCCTCGAGCGTC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTcc                                                                                  >  7:554335/1‑151 (MQ=255)
                                                                    gAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTccc                                                                                 >  6:374472/1‑151 (MQ=255)
                                                                    gAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTccc                                                                                 >  3:1284796/1‑151 (MQ=255)
                                                                    gAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTccc                                                                                 >  4:474962/1‑151 (MQ=255)
                                                                     aaCTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTcccc                                                                                >  7:1245452/1‑151 (MQ=255)
                                                                       ctctCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTAGAGCGTC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTc                                                                                   >  4:1205771/1‑146 (MQ=255)
                                                                        tctcAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCccac                                                                             >  8:211220/1‑151 (MQ=255)
                                                                        tctcAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCccac                                                                             >  5:1691341/1‑151 (MQ=255)
                                                                         ctcAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCccacc                                                                            >  3:1076915/1‑151 (MQ=255)
                                                                           gatattaatttctaaTCAAATATTGTGCCGATGGGGGGAAGGATGAGATGCTTCGACTAAGGTTCGTCTGGAGCGGC‑TGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCccaccac                                                                          <  8:1989255/138‑1 (MQ=11)
                                                                           ccatcGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGTCTCGAGCGTC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCccaccac                                                                          <  2:1186850/147‑1 (MQ=9)
                                                                           cAAGCGATTCCGCATTTAAATATTCTGCCCTTGCGGGGATGTATGAGAAGGTTCGACTAAGGTTCGCCTGGTGCGTC‑AGCGAGAGAGCTTTGCGGGAGGAAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCccacca                                                                           <  8:648012/150‑1 (MQ=11)
                                                                            aatcGAATCCTCAATCAATTTTTCTGCCCATGGGGGGATGGATGAGAAGCTTCGACTAAGGTTGGTCTTGAG‑GTCTAGGGAGTGAGGGTTGCGGCAGGCAAGGACCCGAAGGGGGAAGCGCGCAGCGCTGAGTATTCCTTGCCggaccac                                                                          <  6:14324/147‑1 (MQ=2)
                                                                              agcaacactaattaaaaTATTCTTCCCATCCTCGTAGGTATTCGTAGCTTCGACTAAGGTTCGTCTGGAGCGTC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCAt                                                                       <  2:1969077/137‑1 (MQ=11)
                                                                                  aTCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAgcg                                                                                                        >  1:1428431/1‑114 (MQ=40)
                                                                                   tCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCGCtt                                                                  >  7:1664639/1‑151 (MQ=38)
                                                                                   tCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCACccaccacc                                                                         >  6:823/1‑144 (MQ=38)
                                                                                   tCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGCCTAGAGCGTC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCCCtt                                                                  >  4:891967/1‑151 (MQ=255)
                                                                                    ccGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCGCttc                                                                 >  4:734951/1‑150 (MQ=37)
                                                                                      gttctCATATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCa                                                               <  3:459870/147‑1 (MQ=255)
                                                                                      gCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCCCCATCGCttcct                                                               >  1:943040/1‑148 (MQ=255)
                                                                                        aaTCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCGCTTCCat                                                              >  2:1534448/1‑149 (MQ=255)
                                                                                          tCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCGCttccttaa                                                            >  2:676497/1‑144 (MQ=16)
                                                                                          tCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCCCTTACaaaa                                                            >  6:247654/1‑150 (MQ=255)
                                                                                           cAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCGCttgcttaaat                                                          >  3:134983/1‑143 (MQ=255)
                                                                                              atatTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTGCGGCTCGAGCGGC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGcccc                                                       <  1:538762/151‑1 (MQ=25)
                                                                                                atTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCGCTTCCagatcgcaagc                                                     >  7:645964/1‑141 (MQ=255)
                                                                                                atTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCCCTTCCataaaaaaac                                                      >  2:1634684/1‑144 (MQ=255)
                                                                                                  tCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCGCttccttaaaaaatc                                                      >  5:907215/1‑136 (MQ=255)
                                                                                                    tGCCCAAGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTACaaaaaaaatcaaaa                                                  >  2:760026/1‑140 (MQ=11)
                                                                                                     gCCCATGCGGGTAAGGATGAGAAGCTTCGACTAAGGTTCGTCTCGAGCGGC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATtc                                                <  6:1283309/151‑1 (MQ=255)
                                                                                                     gCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCccacc                                                                            >  5:1105209/1‑123 (MQ=37)
                                                                                                     gCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTACtaaaaaaaacaaaaac                                                >  6:1760696/1‑139 (MQ=11)
                                                                                                     gCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGGC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCAt                                                                       >  3:830624/1‑128 (MQ=21)
                                                                                                     gCCCATGCGGGGAAGGATGAGAAGCTTCGACAAAGGTGCGCCTCGAGCGGC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATtc                                                <  5:1459832/151‑1 (MQ=255)
                                                                                                      cccATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTCATCCTTCCCccacc                                                                            >  1:2059922/1‑122 (MQ=37)
                                                                                                      cccATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGGC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATtct                                               <  7:499656/151‑1 (MQ=255)
                                                                                                      cccATGCGGGGAAGGATGAGAAGCTTCGACCAAGGTTCGGCTCGAGCGCC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATtct                                               <  5:671234/151‑1 (MQ=18)
                                                                                                      cccATGCGGGGAAGGATGAGAAGCTTCCACCAAGGTTCGCCTCGAGCGCC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATtct                                               <  5:508273/151‑1 (MQ=9)
                                                                                                       ccATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTC‑AGCGAGAGAGCGTTGCCGCa                                                                                                                                <  6:1065163/69‑1 (MQ=255)
                                                                                                         aTGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGAACCGGAATTCTCa                                             >  4:1712980/1‑150 (MQ=255)
                                                                                                           gCGGGTAAGGATTATAAGCTTCTACAAAGGTTCGGCTCGAGCGCC‑AGCGATAGAGCGTTGCCGCAGGAAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAAc                                          <  8:1078848/151‑1 (MQ=11)
                                                                                                           gCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGCCTCGAGCGCC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAAc                                          <  3:250992/151‑1 (MQ=255)
                                                                                                           gCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGACTCGAGCGTC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAAc                                          <  2:907370/151‑1 (MQ=255)
                                                                                                             ggggAAGGATGAGAAGCTTCGACTAAGGTTCGTCTCGAGCGGC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAACGa                                        <  2:308363/151‑1 (MQ=255)
                                                                                                                  aGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCCCCATCCCTTTCATAAGCACAGCAATCCTCTAACGCAGCCg                                   >  1:39986/1‑151 (MQ=14)
                                                                                                                        agaAGCTTCGACTAAGGTTCGTCTCGAGCGTC‑AGGGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAACGAATCCGCAATc                              <  8:887749/150‑1 (MQ=255)
                                                                                                                        agaAGCTTCGACTAAGGTTCGGCTCGAGCGTC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCCCCACCAGCCCTTTCAAAAGCCCCGGAATTCTCAAACGCATCCGCAATc                              >  3:559580/1‑150 (MQ=255)
                                                                                                                             cgacgtgtccggTTCGGCTCTAGCGCC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAACGAATCCGCAATCAAatat                        <  7:1610169/141‑1 (MQ=255)
                                                                                                                              ttCGACTAAGGTTCGGCTCGAGCGTC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCGCTTGCTAAAGCAAACCAAAACTCAAACGAAGCCGCAATGaactcgt                       >  1:1688444/1‑146 (MQ=14)
                                                                                                                               tCGACTAAGGTTCGGCTCGAGCGTC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCCCCATCACTTTCAAAAGCCCCGGAATTCTCAAACGAATCCGCAATCAAATAtt                       >  1:1483741/1‑150 (MQ=255)
                                                                                                                               gcGACAAAGGTTCGGCTCGAGCGCC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAACGAATCCGCAATCAAATATTc                      <  7:138335/150‑1 (MQ=255)
                                                                                                                                 gACTAAGGTTCGCCTCGAGCGTC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAACGAATCCGCAATCAAATATTCTg                    <  2:479584/151‑1 (MQ=255)
                                                                                                                                     aaGGTTCGGCTCGAGCGTC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAAAGCGCAGCGCTGAGTAATCCTTACCCCACCAACAAGAATTTAAAAAGCCCCGGAAATCTCAAACGAATAAGAAATCAAATATTCTGccc                 >  2:1335578/1‑150 (MQ=14)
                                                                                                                                       ggTTCGGCTCGAGCGTC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAACGAATCCGCAATCAAATATTCTGCCCa                >  7:664791/1‑149 (MQ=255)
                                                                                                                                             gCTCGAGCGTC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAACGAATCCGCAATCAAATATTCTGCCCAAGCgggg         >  3:1972673/1‑150 (MQ=255)
                                                                                                                                                cGAGCGGC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAACGAATCCGCAATCAAATATTCTGCCCAAGCGGGGAAg      <  2:414989/150‑1 (MQ=255)
                                                                                                                                                cGAGCGCC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAACGAATCCGCAATCAAATATTCTGCCCAAGCGGGGAAgg     <  1:1246331/151‑1 (MQ=38)
                                                                                                                                                 gAGCGTC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCGCTTTCTAAAGCACCGGAAAGCTCTAACGCCGCCGGAATGATAGATTCTGCCCCAGCGGTGaacgg    >  1:7618/1‑148 (MQ=14)
                                                                                                                                                      |                                                                                                                                                    
GACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTC‑AGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAACGAATCCGCAATCAAATATTCTGCCCAAGCGGGGAAGGATG  >  DH1/2591363‑2591660

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑