Predicted mutation
evidence seq id position mutation annotation gene description
RA DH1 3,970,143 G→T E6* (GAG→TAG)  ECDH1_RS19620 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH13,970,1430GT99.0% 2347.2 / 5.2 619E6* (GAG→TAG) ECDH1_RS19620hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (3/3);  new base T (297/316);  total (300/319)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 619 total aligned reads displayed.

GCCAACACTGCACAGTTTTCCCCCAAAAGATGAAACATTTGGGGCCATTTTGACTCATAGAGGAAAGCATCGCGGACAAACTTTTTCAGTTTATTTGTTGGCTTAATATTCTATTGTTATCTTTATTTATAGATGTTTATATTGCATGAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACGTTAGCTTTACATATAGCGGAGGTGATGTGAAATTAATTTACAATAGAAATAATTTACATATCAAACAGTTAGATGCTTTTTGTCGTTTTTTAATAT  >  DH1/3969996‑3970290
                                                                                                                                                   |                                                                                                                                                   
gCCAACACTGCACAGTTTTCCCCCAAAAGATGAAACATTTGGGGCCATTTTGACTCATAGAGGAAAGCATCGCGGACAAACTTTTTCAGTTTATTTGTTGGCTTAATATTCTATTGTTATCTTTATTTATAGATGTTTATATTGCATTAg                                                                                                                                                   <  1:11145/150‑1 (MQ=255)
 ccAACACTGCACAGTTTTCCCCCAAAAGATGAAACATTTGGGGCCATTTTGACTCATAGAGGAAAGCATCGCGGACAAACTTTTTCAGTTTATTTGTTGGCTTAATATTCTATTGTTATCTTTATTTATAGATGTTTATATTGCATTAgg                                                                                                                                                  <  4:165235/150‑1 (MQ=255)
  cAACACTGCACAGTTTTCCCCCAAAAGATGAAACATTTGGGGCCATTTTGACTCATAGAGGAAAGCATCGCGGACAAACTTTTTCAGTTTATTTGTTGGCTTAATATTCTATTGTTATCTTTATTTATAGATGTTTATATTGCATTAggtg                                                                                                                                                >  3:1067556/1‑151 (MQ=255)
   aaCACTGCACAGTTTTCCCCCAAAAGATGAAACATTTGGGGCCATTTTGACTCATAGAGGAAAGCATCGCGGACAAACTTTTTCAGTTTATTTGTTGGCTTAATATTCTATTGTTATCTTTATTTATAGATGTTTATATTGCATTAggtgg                                                                                                                                               <  2:1923844/151‑1 (MQ=255)
     cacTGCACAGTTTTCCCCCAAAAGATGAAACATTTGGGGCCATTTTGACTCATAGAGGAAAGCATCGCGGACAAACTTTTTCAGTTTATTTGTTGGCTTAATATTCTATTGTTATCTTTATTTATAGATGTTTATATTGCATTAGGTGGtt                                                                                                                                             >  7:1722580/1‑151 (MQ=255)
      acTTCACAGTTTTCCCCCAAAAGATGAAACATTTGGGGCCATTTTGACTCATAGAGGAAAGCATCGCGGACAAACTTTTTCAGTTTATTTGTTGGCTTAATATTCTATTGTTATCTTTATTTATAGATGTTTATATTGCATTAGGTGGttt                                                                                                                                            <  1:1830788/151‑1 (MQ=255)
         gCACAGTTTTCCCCCAAAAGATGAAACATTTGGGGCCATTTTGACTCATAGAGGAAAGCATCGCGGACAAACTTTTTCAGTTTATTTGTTGGCTTAATATTCTATTGTTATCTTTATTTATAGATGTTTATATTGCATTAGGTGGttttt                                                                                                                                          <  1:1013459/150‑1 (MQ=255)
                 ttCCCCCAAAAGATGAAACATTTGGGGCCATTTTGACTCATAGAGGAAAGCATCGCGGACAAACTTTTTCAGTTTATTTGTTGGCTTAATATTCTATTGTTATCTTTATTTATAGATGTTTATATTGCATTAGGTGGTTTTTGgagaga                                                                                                                                   >  8:1629902/1‑149 (MQ=255)
                    ccccAAAAGTTGAAACATTTGGGGCCATTTTGACTCATAGAGGAAAGCATCGCGGAGAAACTTTTTCAGTTTATTTGTTGGCTTAATATTCTATTGTTATCTTTATTTATAGATGTTTATATTGAATTAGGTGGTTTTTGGAGAGAAGAAt                                                                                                                              <  7:1397107/151‑1 (MQ=255)
                    ccccAAAAGATGAAACATTTGGGGCCATTTTGACTCATAGAGGAAAGCATCGCGGACAAACTTTTTCAGTTTATTTGTTGGCTTAATATTCTATTGTTATCTTTATTTATAGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAAt                                                                                                                              <  1:1629377/151‑1 (MQ=255)
                     cccAAAAGATGAAACATTTGGGGCCATTTTGACTCATAGAGGAAAGCATCGCGGACAAACTTTTTCAGTTTATTTGTTGGCTTAATATTCTATTGTTATCTTTATTTATAGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATg                                                                                                                             <  5:1803284/151‑1 (MQ=255)
                             aTGAAACATTTGGGGCCATTTTGACTCATAGAGGAAAGCATCGCGGACAAACTTTTTCAGTTTATTTGTTGGCTTAATATTCTATTGTTATCTTTATTTATAGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGa                                                                                                                         >  8:1028906/1‑147 (MQ=255)
                              tGAAACATTTGGGGCCATTTTGACTCATAGAGGAAACCATCGCGGACAAACTTTTTCAGTTTATTTGTTGGCTTAATATTCTATTGTTATCTTTATTTATAGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGAt                                                                                                                     >  6:1674117/1‑150 (MQ=255)
                               gAAACATTTGGGGCCATTTTGACTCATAGAGGAAAGCATCGCGGACAAACTTTTTCAGTTTATTTGATGGCTTAATATTCTATTGTTATCTTTATTTATAGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGc                                                                                                                   >  5:1185181/1‑151 (MQ=255)
                                 aaCATTTGGGGCCATTTTGACTCATAGAGGAAAGCATCGCGGACAAACTTTTTCAGTTTATTTGTTGGCTTAATATTCTATTGTTATCTTTATTTATAGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGt                                                                                                                 >  1:1979292/1‑151 (MQ=255)
                                   cATTTGGGGCCATTTTGACTCATAGAGGAAAGCATCGCGGACAAACTTTTTCAGTTTATTTGTTGGCTTAATATTCTATTGTTATCTTTATTTATAGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCg                                                                                                               <  3:221937/151‑1 (MQ=255)
                                   cATTTGGGGCCATTTTGACTCATAGAGGAAAGCATCGCGGACAAACTTTTTCAGTTTATTTGTTGGCTTAATATTCTATTGTTATCTTTATTTATAGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCg                                                                                                               >  5:103223/1‑151 (MQ=255)
                                    aTTTGGGGCCATTTTGACTCATAGAGGAAAGCATCGCGGACAAACTTTTTCAGTTTATTTGTTGGCTTAATATTCTATTGTTATCTTTATTTATAGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGa                                                                                                              <  3:318553/151‑1 (MQ=255)
                                      ttGGGGCCATTTTGACTCATAGAGGAAAGCATCGCGGACAAACTTTTTCAGTTTATTTGTTGGCTTAATATTCTATTGTTATCTTTATTTATAGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCg                                                                                                               >  8:1424583/1‑148 (MQ=255)
                                      ttGGGGCCATTTTGACTCATAGAGGAAAGCATCGCGGACAAACTTTTTCAGTTTATTTGTTGGCTTAATATTCTATTGTTATCTTTATTTATAGATGTTTATATTGCATGAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAg                                                                                                             <  7:1509813/150‑1 (MQ=255)
                                        ggggCCATTTTGACTCATAGAGGAAAGCATCGCGGACAAACTTTTTCAGTTTATTTGTTGGCTTAATATTCTATTGTTATCTTTATTTATAGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCca                                                                                                          <  3:574728/151‑1 (MQ=255)
                                         gggCCATTTTGACTCATAGAGGAAAGCATCGCGGACAAACTTTTTCAGTTTATTTGTTGGCTTAATATTCTATTGTTATCTTTATTTATAGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCcac                                                                                                         <  2:543011/151‑1 (MQ=255)
                                          ggCCATTTTGACTCATAGAGGAAAGCATCGCGGACAAACTTTTTCAGTTTATTTGTTGGCTTAATATTCTATTGTTATCTTTATTTATAGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCcaca                                                                                                        <  3:441037/151‑1 (MQ=255)
                                                 ttGACTCATAGAGGAAAGCATCGCGGACAAACTTTTTCAGTTTATTTGTTGGCTTAATATTCTATTGTTATCTTTATTTATAGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACg                                                                                                  <  6:2008507/150‑1 (MQ=255)
                                                 ttGACTCATAGAGGAAAGCATCGCGGACAAACTTTTTCAGTTTATTTGTTGGCTTAATATTCTATTGTTATCTTTATTTATAGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACg                                                                                                  <  4:186948/150‑1 (MQ=255)
                                                   gACTCATATAGGAAAGCATCGCGGACAAACTTTTTCAGTTTATTTGTTGGCTTAATATTATATTGTTATCTTTATTTATAGATGTTTATATTTCATTAGGTGGTTTTTGGAGAGAAGAATGATGAAGATGCGTCTAGACACAAAAACgat                                                                                                >  6:460395/1‑148 (MQ=255)
                                                   gACTCATAGAGGAAAGCATCGCGGACAAACTTTTTCAGTTTATTTGTTGGCTTAATATTCTATTGTTATCTTTATTTATAGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACGTTa                                                                                               <  2:1122401/151‑1 (MQ=255)
                                                         tAGAGGAAAGCATCGCGGACAAACTTTTTCAGTTTATTTGTTGGCTTAATATTCTATTGTTATCTTTATTTATAGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACGTTAGCttt                                                                                          >  1:382113/1‑150 (MQ=255)
                                                          agagGAAAGCATCGCGGACAAACTTTTTCAGTTTATTTGTTGGCTTAATATTCTATTGTTATCTTTATTTATAGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACGTTAGCTTTAc                                                                                        >  4:809443/1‑151 (MQ=255)
                                                           gagGAAAGCATCGCGGACAAACTTTTTCAGTTTATTTGTTGGCTTAATATTCTATTGTTATCTTTATTTATAGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACGTTAGCTTTAc                                                                                        >  5:605416/1‑150 (MQ=255)
                                                           gagGAAAGCATCGCGGACAAACTTTTTCAGTTTATTTGTTGGCTTAATATTCTATTGTTATCTTTATTTATAGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACGTTAGCTTTAc                                                                                        >  6:570879/1‑150 (MQ=255)
                                                             ggAAAGCATCGCGGACAAACTTTTTCAGTTTATTTGTTGGCTTAATATTCTATTGTTATCTTTATTTATAGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACGTTAGCTTTACata                                                                                     <  4:279905/151‑1 (MQ=255)
                                                               aaaGCATCGCGGACAAACTTTTTCAGTTTATTTGTTGGCTTAATATTCTATTGTTATCTTTATTTATAGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACGTTAGCTTTACAtata                                                                                   <  8:341446/151‑1 (MQ=255)
                                                                  gCATCGCCGACAAACTTTTTCAGTTTATTTGTTGGCTTAATATTCTATTGTTATCTTTATTTATAGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACGTTAGCTTTACATATAGCg                                                                                <  1:1463670/151‑1 (MQ=255)
                                                                   cATCGCGGACAAACTTTTTCAGTTTATTTGTTGCCTTAATATTCTATTGTTATCTTTATTTATAGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACGTTAGCTTTACATATAGCgg                                                                               <  2:275407/151‑1 (MQ=255)
                                                                    aTCGCGGACAAACTTTTTCAGTTTATTTGTTGGCTTAATATTCTATTGTTATCTTTATTTATAGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACGTTAGCTTTACATATAGCgg                                                                               >  6:1354342/1‑150 (MQ=255)
                                                                    aTCGCGGACAAACTTTTTCAGTTTATTTGTTGGCTTAATATTCTATTGTTATCTTTATTTATAGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACGTTAGCTTTACATATAGCgg                                                                               >  6:882203/1‑150 (MQ=255)
                                                                      cgcgGACAAACTTTTTCAGTTTATTTGTTGGCTTAATATTCTATTGTTATCTTTATTTATAGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACGTTAGCTTTACATATAGCGGAgg                                                                            >  2:1078523/1‑151 (MQ=255)
                                                                      cgcgGACAAACTTTTTCAGGTTATTTGTTGGCTTAATATTCTATTGTTATCTTTATTTATAGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACGTTAGCTTTACATATAGCGGAg                                                                             <  4:147660/150‑1 (MQ=255)
                                                                       gcgGACAAACTTTTTCAGTTTATTTGTTGGCTTAATATTCTATTGTTATCTTTATTTATAGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACGTTAGCTTTACATATAGCGGAGGt                                                                           <  8:812484/151‑1 (MQ=255)
                                                                        cgGACAAACTTTTTCAGTTTATTTGTTGGCTTAATATTCTATTGTTATCTTTATTTATAGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACGTTAGCTTTACATATAGCGGAGGTg                                                                          >  5:680090/1‑151 (MQ=255)
                                                                         ggACAAACTTTTTCAGTTTATTTGTTGGCTTAATATTCTATTGTTATCTTTATTTATAGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACGTTAGCTTTACATATAGCGGAGGTGa                                                                         <  4:1035696/151‑1 (MQ=255)
                                                                         ggACAAACTTTTTCAGTTTATTTGTTGGCTTAATATTCTATTGTTATCTTTATTTATAGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACGTTAGCTTTACATATAGCGGAGGTGa                                                                         <  8:516073/151‑1 (MQ=255)
                                                                          gACAAACTTTTTCAGTTTATTTGTTGGCTTAATATTCTATTGTTATCTTTATTTATAGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACGTTAGCTTTAAATATAGCGGAGGTGAt                                                                        >  3:76034/1‑151 (MQ=255)
                                                                           aCAAACTTTTTCAGTTTATTTGTTGGCTTAATATTCTATTGTTATCTTTATTTATAGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACGTTAGCTTTACATATAGCGGAGGTGAtg                                                                       <  3:1492991/151‑1 (MQ=255)
                                                                             aaaCTTTTTCAGTTTATTTGTTGGCTTAATATTCTATTGTTATCTTTATTTATAGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACGTTAGCTTTACATATAGCGGAGGTGAtgtg                                                                     >  8:2044625/1‑151 (MQ=255)
                                                                             aaaCTTTTTCAGTTTATTTGTTGGCTTAATATTCTATTGTTATCTTTATTTATAGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACGTTAGCTTTACATATAGCGGAGGTGAtgtg                                                                     <  7:1624386/151‑1 (MQ=255)
                                                                              aaCTTTTTCAGTTTATTTGTTGGCTTAATATTCTATTGTTATCTTTATTTATAGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACGTTAGCTTTACATATAGCGGAGGTGATGTGa                                                                    <  4:858171/151‑1 (MQ=255)
                                                                              aaCTTTTTCAGTTTATTTGTTGGCTTAATATTCTATTGTTATCTTTATTTATAGATCTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACGTTAGCTTTACATATAGCGTAGGTGAtgtg                                                                     >  1:993716/1‑150 (MQ=255)
                                                                               aCTTTTTCAGTTTATTTGTTGGCTTAATATTCTATTGTTATCTTTATTTATAGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACGTTAGCTTTACATATAGCGGAGGTGATGTGaa                                                                   <  8:1558103/151‑1 (MQ=255)
                                                                                  ttttCAGTTTATTTGTTGGCTTAATATTCTATTGTTATCTTTATTTATAGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACGTTAGCTTTACATATAGCGGAGGTGATGTGAaatt                                                                >  5:1315695/1‑151 (MQ=255)
                                                                                   tttCAGTTTATTTGTTGGCTTAATATTCTATTGTTATCTTTATTTATAGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACGTTAGCTTTACATATAGCGGAGGTGATGTGAaatt                                                                <  4:630313/150‑1 (MQ=255)
                                                                                      cAGTTTATTTGTTGGCTTAATATTCTATTGTTATCTTTATTTATAGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACGTTAGCTTTACATATAGCGGAGGTGATGTGAaattaatt                                                            <  6:1102307/151‑1 (MQ=255)
                                                                                       aGTTTATTTGTTGGCTTAATATTCTATTGTTATCTTTATTTATAGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACGTTAGCTTTACATATAGCGGAGGTGATGTGAAATTAAttt                                                           >  6:240576/1‑151 (MQ=255)
                                                                                       aGTTTATTTGTTGGCTTAATATTCTATTGTTATCTTTATTTATAGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACGTTAGCTTTACATATAGCGGAGGTGATGTGAAATTAAttt                                                           >  4:1127827/1‑151 (MQ=255)
                                                                                        gTTTATTTGTTGGCTTAATATTCTATTGTTATCTTTATTTATAGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACGTTAGCTTTACATATAGCGGAGGTGATGTGAAATTAATTTa                                                          <  5:49399/151‑1 (MQ=255)
                                                                                         tttATTTGTTGGCTTAATATTCTATTGTTATCTTTATTTATAGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACGTTAGCTTTACATATAGCGGAGGTGATGTGAAATTAATTTAc                                                         >  7:596146/1‑151 (MQ=255)
                                                                                           tATTTGTTGGCTTAATATTCTATTGTTATCTTTATTTATAGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACGTTAGCTTTACATATAGCGGAGGTGATGTGAAATTAATTTACa                                                        >  7:756365/1‑150 (MQ=255)
                                                                                           tATTTGTTGGCTTAATATTCTATTGTTATCTTTATTTATAGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACGTTAGCTTTACATATAGCGGAGGTGATGTGAAATTAATTTACa                                                        <  3:1217326/150‑1 (MQ=255)
                                                                                            aTTTTTTGGCTTAATATTCTATTGTTATCTTTATTTATACATTGTTATATTGCATTACGTGGTTTTTGGAGACAACAATGCCGAAGAAGCGGCGACCCACACAAACGTTAGCTTTACATATAGCGGAGGTGATGTGAAATTAATTTACaa                                                       <  1:860518/150‑1 (MQ=255)
                                                                                            aTTTGTTGGCTTAATATTCTATTGTTATCTTTATTTATAGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACGTTAGCTTTACATATAGCGGAGGTGATGTGAAATTAATTTACaa                                                       <  3:174686/150‑1 (MQ=255)
                                                                                            aTTTGTTGGCTTAATATTCTATTGTTATCTTTATTTATAGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACGTTAGCTTTACATATAGCGGAGGTGATGTGAAATTAATTTACAAt                                                      <  5:321282/151‑1 (MQ=255)
                                                                                             tttGTTGGCTTAATATTCTATTGTTATCTTTATTTATAGTTGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATTAGGAAGATGCGTCGAGCCACAGAAACGTTAGCTTTACATATAGCGGAGGTGATGTGAAATTAATTTACAAt                                                      <  4:2043314/150‑1 (MQ=255)
                                                                                               tgttgGCTTAATATTCTATTGTTATCTTTATTTATAGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGCGCCACAGAAACGTTAGCTTTACATATAGCGGAGGTGATGTGAAATTAATTTACAAt                                                      >  2:1311837/1‑148 (MQ=255)
                                                                                                gttgGCTTAATATTCTATTGTTATCTTTATTTATAGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACGTTAGCTTTACATATAGCGGAGGTGATGTGAAATTAATTTACAATAGaa                                                  <  1:702246/151‑1 (MQ=255)
                                                                                                gttgGCTTAATATTCTATTGTTATCTTTATTTATAGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACGTTAGCTTTACATATAGCGGAGGTGATGTGAAATTAATTTACAATAGa                                                   >  5:1677748/1‑150 (MQ=255)
                                                                                                gttgGCTTAATATTCTATTGTTATCTTTATTTATAGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACGTTAGCTTTACATATAGCGGAGGTGATGTGAAATTAATTTACAATAGa                                                   <  6:1543240/150‑1 (MQ=255)
                                                                                                    gCTTAATATTCTATTGTTATCTTTATTTATAGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACGTTAGCTTTACATATAGCGGAGGTGATGTGAAATTAATTTACAATAGAaata                                               >  1:241046/1‑150 (MQ=255)
                                                                                                     cTTAATATTCTATTGTTATCTTTATTTATAGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACGTTAGCTTTACATATAGCGGAGGTGATGTGAAATTAATTTACAATAGAaataat                                             >  2:1653361/1‑151 (MQ=255)
                                                                                                     cTTAATATTCTATTGTTATCTTTATTTATAGATGTTTATATTGCATGAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACGTTAGCTTTACATATAGCGGAGGTGATGTGAAATTAATTTACAATAGAaataat                                             >  7:1859371/1‑151 (MQ=255)
                                                                                                       tAATATTCTATTGTTATCTTTATTTATAGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACGTTAGCTTTACATATAGCGGAGGTGATGTGAAATTAATTTACAATAGAAATAAtt                                            <  7:1028926/150‑1 (MQ=255)
                                                                                                        aaTATTCTATTGTTATCTTTATTTATAGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACGTTAGCTTTACATATAGCGGAGGTGATGTGAAATTAATTTACAATAGAAATAAttt                                           <  6:1743091/150‑1 (MQ=255)
                                                                                                        aaTATTCTATTGTTATCTTTATTTATAGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACGTTAGCTTTACATATAGCGGAGGTGATGTGAAATTAATTTACAATAGAAATAAttt                                           >  4:1793705/1‑150 (MQ=255)
                                                                                                         atatTCTATTGTTATCTTTATTTATAGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACGTTAGCTTTACATATAGCGGAGGTGATGTGAAATTAATTTACAATAGAAATAATTTAc                                         <  2:734013/151‑1 (MQ=255)
                                                                                                             tCTATTGTTATCTTTATTTATAGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACGTTAGCTTTACATATAGCGGAGGTGATGTGAAATTAATTTACAATAGAAATAATTTACatat                                     >  3:1631105/1‑151 (MQ=255)
                                                                                                                aTTGTTATCTTTATTTATAGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACGTTAGCTTTACATATAGCGGAGGTGATGTGAAATTAATTTACAATAGAAATAATTTACATATCaa                                  <  5:1539118/151‑1 (MQ=255)
                                                                                                                  tgctATCTTTATTTATAGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACGTTAGCTTTACATATAGCGGAGGTGATGTGAAATTAATTTACAATAGAAATAATTTACATATCaaa                                 <  4:1914178/147‑1 (MQ=255)
                                                                                                                   gTTATCTTTATTTATAGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACGTTAGCTTTACATATAGCGGAGGTGATGTGAAATTAATTTACAATAGAAATAATTTACATATCAAAc                                >  4:394543/1‑150 (MQ=255)
                                                                                                                   gTTATCTTTATTTATAGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACGTTAGCTTTACATATAGCGGAGGTGATGTGAAATTAATTTACAATAGAAATAATTTACATATCAAAc                                <  2:1479954/150‑1 (MQ=255)
                                                                                                                   gTTATCTTTATTTATAGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACGTTAGCTTTACATATAGCGGAGGTGATGTGAAATTAATTTACAATAGAAATAATTTACATATCAAACa                               <  4:1244370/151‑1 (MQ=255)
                                                                                                                     tATCTTTATTTATAGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACGTTAGCTTTACATATAGCGGAGGTGATGTGAAATTAATTTACAATAGAAATAATTTACATATCAAACAGt                             >  1:563985/1‑151 (MQ=255)
                                                                                                                      aTCTTTATTTATAGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACGTTAGCTTTACATATAGCGGAGGTGATGTGAAATTAATTTACAATAGAAATAATTTACATATCAAACAGtt                            >  3:1341612/1‑151 (MQ=255)
                                                                                                                       tCTTTATTTATAGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACGTTAGCTTTACATATAGCGGAGGTGATGTGAAATTAATTTACAATAGAAATAATTTACATATCAAACAGtt                            >  2:1762215/1‑150 (MQ=255)
                                                                                                                        gtttatttaTAGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACGTTAGCTTTACATATAGCGGAGGTGATGTGAAATTAATTTACAATAGAAATAATTTACATATCAAACAGTTAg                          <  5:247539/150‑1 (MQ=255)
                                                                                                                        cTTTATTTATAGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACGTTAGCTTTACATATAGCGGAGGTGATGTGAAATTAATTTACAATAGAAATAATTTACATATCAAACAGtt                            >  1:1712565/1‑149 (MQ=255)
                                                                                                                            atttatAGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACGTTAGCTTTACATATAGCGGAGGTGATGTGAAATTAATTTACAATAGAAATAATTTACATATCAAACAGTTAGATGc                      >  5:2044532/1‑151 (MQ=255)
                                                                                                                            atttatAGATGTTTATATAGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACGTTAGCTTTACATATACCGGAGGTGAAGTGAAATTAATTTACAATATAAATAATTTACATATCAAACAGTTAGATg                       >  8:656908/1‑150 (MQ=255)
                                                                                                                               tataGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACGTTAGCTTTACATATAGCGGAGGTGATGTGAAATTAATTTACAATAGAAATAATTTACATATCAAACAGTTAGATGCttt                   >  7:479768/1‑151 (MQ=255)
                                                                                                                               tataGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACGTTAGCTTTACATATAGCGGAGGTGATGTGAAATTAATTTACAATAGAAATAATTTACATATCAAACAGTTAGATGCttt                   >  8:1807485/1‑151 (MQ=255)
                                                                                                                               tataGATGTTTATATTCCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACGTTAGATATACATATAGCGGACGTGATGAGAAATTAATTTACAATACAAATAATTTACATATCAAACACTTAGATGAttt                   >  6:698419/1‑151 (MQ=255)
                                                                                                                                 taGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACGTTAGCTTTACATATAGCGGAGGTGATGTGAAATTAATTTACAATAGAAATAATTTACATATCAAACAGTTAGATGCttttt                 >  2:896133/1‑151 (MQ=255)
                                                                                                                                  aGATGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACGTTAGCTTTACATATAGCGGAGGTGATGTGAAATTAATTTACAATAGAAATAATTTACATATCAAACAGTTAGATGCTTTTTg                <  7:2053970/151‑1 (MQ=255)
                                                                                                                                    aTGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACGTTAGCTTTACATATAGCGGAGGTGATGTGAAATTAATTTACAATAGAAATAATTTACATATCAAACAGTTAGATGCTTTTTGt               <  8:130300/150‑1 (MQ=255)
                                                                                                                                    aTGTTTATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACGTTAGCTTTACATATAGCGGAGGTGATGTGAAATTAATTTACAATAGAAATAATTTACATATCAAACAGTTAGATGCTTTTTGt               <  3:1902738/150‑1 (MQ=255)
                                                                                                                                       tttATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACGTTAGCTTTACATATAGCGGAGGTGATGTGAAATTAATTTACAATAGAAATAATTTACATATCAAACAGTTAGATGCTTTTTGTCGtt           >  1:1359148/1‑151 (MQ=255)
                                                                                                                                        ttATATTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACGTTAGCTTTACATATAGCGGAGGTGATGTGAAATTAATTTACAATAGAAATAATTTACATATCAAACAGTTAGATGCTTTTTGTCGtt           <  4:1331523/150‑1 (MQ=255)
                                                                                                                                         tataTTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACGTTAGCTTTACATATAGCGGAGGTGATGTGAAATTAATTTACAATAGAAATAATTTACATATCAAACAGTTAGATGCTTTTTGTCGtttt         >  5:1399545/1‑151 (MQ=255)
                                                                                                                                            atTGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACGTTAGCTTTACATATAGCGGAGGTGATGTGAAATTAATTTACAATAGAAATAATTTACATATCAAACAGTTAGATGCTTTTTGTCGTTTTTTa      <  5:882609/151‑1 (MQ=255)
                                                                                                                                              tGCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACGTTAGCTTTACATATAGCGGAGGTGATGTGAAATTAATTTACAATAGAAATAATTTACATATCAAACAGTTAGATGCTTTTTGTCGTTTTTTAat    >  7:1156341/1‑151 (MQ=255)
                                                                                                                                               gCATTAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACGTTAGCTTTACATATAGCGGAGGTGATGTGAAATTAATTTACAATAGAAATAATTTACATATCAAACAGTTAGATGCTTTTTGTCGTTTTTTAata   <  1:441824/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GCCAACACTGCACAGTTTTCCCCCAAAAGATGAAACATTTGGGGCCATTTTGACTCATAGAGGAAAGCATCGCGGACAAACTTTTTCAGTTTATTTGTTGGCTTAATATTCTATTGTTATCTTTATTTATAGATGTTTATATTGCATGAGGTGGTTTTTGGAGAGAAGAATGAGGAAGATGCGTCGAGCCACAGAAACGTTAGCTTTACATATAGCGGAGGTGATGTGAAATTAATTTACAATAGAAATAATTTACATATCAAACAGTTAGATGCTTTTTGTCGTTTTTTAATAT  >  DH1/3969996‑3970290

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑