Predicted mutation
evidence seq id position mutation annotation gene description
RA DH1 3,668,924 (T)5→6 pseudogene (353/455 nt) lpxA ← hydroxymyristoyl‑ACP dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH13,668,9191.T95.0% 1319.0 / 50.5 379pseudogene (358/455 nt)lpxAhydroxymyristoyl‑ACP dehydratase
Reads supporting (aligned to +/- strand):  ref base . (17/2);  new base T (177/183);  total (194/185)
Fisher's exact test for biased strand distribution p-value = 5.78e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 411 total aligned reads displayed.

CCCTCTTCCACAATGGCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGCCAGAATACCTGTTGCCTGT  >  DH1/3668769‑3669064
                                                                                                                                                        |                                                                                                                                                  
cccTCTTCCACAATGGCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGccgcgccgcg                                                                                                                                                     >  8:541605/1‑151 (MQ=255)
 ccTCTTCCACAATGGCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGt                                                                                                                                                   >  3:193537/1‑151 (MQ=255)
      tCCACAATGGCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGACCGCGGCGCGttttt                                                                                                                                              <  7:398499/150‑1 (MQ=255)
          caATGGCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGttttttcgaa                                                                                                                                         <  2:947826/151‑6 (MQ=255)
           aaTGGCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCACCCCCTTTAAAACGGGTCAGGCCGCGGCGCGttttttcgaa                                                                                                                                         >  8:862085/1‑145 (MQ=255)
             tGGCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGt                                                                                                                                       >  5:83758/1‑151 (MQ=255)
              ggCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTg                                                                                                                                      <  6:708616/151‑1 (MQ=255)
               gCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTg                                                                                                                                      >  2:716756/1‑150 (MQ=255)
                    tGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTcc                                                                                                                                >  5:121081/1‑151 (MQ=255)
                     ggATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTcc                                                                                                                                <  2:1224614/150‑1 (MQ=255)
                     ggATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCa                                                                                                                               <  4:528271/151‑1 (MQ=255)
                       aTGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACATCCATg                                                                                                                             >  8:576602/1‑151 (MQ=255)
                           acaAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATc                                                                                                                          >  1:561868/1‑150 (MQ=255)
                             aaaGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGCCTTCCATGATCAtt                                                                                                                       >  6:1269343/1‑151 (MQ=255)
                             aaaGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCAtt                                                                                                                       >  6:1063944/1‑151 (MQ=255)
                              aaGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCAttt                                                                                                                      >  1:632123/1‑151 (MQ=255)
                              aaGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCAttt                                                                                                                      >  2:1336562/1‑151 (MQ=255)
                              aaGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCAttt                                                                                                                      <  5:1354898/151‑1 (MQ=255)
                                  cGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATc                                                                                                                  <  4:37651/151‑1 (MQ=255)
                                  cGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATc                                                                                                                  <  5:680977/151‑1 (MQ=255)
                                   ggATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCg                                                                                                                 >  7:1607924/1‑151 (MQ=255)
                                   ggATTTATCAATCACGTATCACGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCg                                                                                                                 <  2:1028686/151‑1 (MQ=255)
                                     aTTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGcc                                                                                                               <  5:487136/151‑1 (MQ=255)
                                        tATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAgg                                                                                                            >  8:356591/1‑151 (MQ=255)
                                         atcaatcaCGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGc                                                                                                           <  2:1521315/151‑1 (MQ=255)
                                           cactcaCGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCAc                                                                                                         <  6:1379934/148‑1 (MQ=255)
                                            aatcaCGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGGTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACg                                                                                                        >  5:1561864/1‑151 (MQ=255)
                                            aatcaCGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACg                                                                                                        >  5:1142690/1‑151 (MQ=255)
                                            aatcaCGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTAAACCATCGACAAGAGCAACCCCATTAAAACGGGACAGGCCGCGGCGAGTTTAATCGAAAGTGACTACCATCATCATTTGATCTCAAAGCAcc                                                                                                        >  6:1571500/1‑150 (MQ=255)
                                               caCGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGAcc                                                                                                     <  2:803276/151‑1 (MQ=255)
                                                aCGTATCAGGCCTCCCGGCTACTAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCg                                                                                                    <  7:1498692/151‑1 (MQ=255)
                                                 cGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCgg                                                                                                   <  7:1229628/151‑1 (MQ=255)
                                                 cGTATCAGGCCTCCCGGCTACGAGCACACATCATCGATGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGCGTCAGCCCGCGGCGCGTTTTTTCGAAACTGACTTCCATCATCATTTGATCGCCAGGCACGACCgg                                                                                                   >  6:506610/1‑151 (MQ=255)
                                                    aTCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGgcg                                                                                                >  2:1450967/1‑151 (MQ=255)
                                                    aTCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGgcg                                                                                                >  6:779255/1‑151 (MQ=255)
                                                     tCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCGATGATCATTTGATCGCCAGGCACGCCCGGgcgc                                                                                               >  6:1636439/1‑151 (MQ=255)
                                                       aGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCtt                                                                                             >  1:239460/1‑151 (MQ=255)
                                                       aGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCtt                                                                                             <  1:1488483/151‑1 (MQ=255)
                                                         gCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGa                                                                                           <  7:740952/151‑1 (MQ=255)
                                                          ccTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGTCTTCCATGATCATTTGATCGCCAGGCACGCCCGGGCGCTTGa                                                                                           >  1:774489/1‑150 (MQ=255)
                                                          ccTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGaa                                                                                          <  7:1002336/151‑1 (MQ=255)
                                                           cTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGa                                                                                           >  4:1184383/1‑149 (MQ=255)
                                                            tCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAgc                                                                                        >  3:603707/1‑151 (MQ=255)
                                                             cccGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAgcg                                                                                       <  3:33961/151‑1 (MQ=255)
                                                                 gCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCt                                                                                   >  3:642801/1‑151 (MQ=255)
                                                                  cTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTc                                                                                 >  5:1419496/1‑151 (MQ=255)
                                                                    aCGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCg                                                                                <  5:1091776/151‑1 (MQ=255)
                                                                     cGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGt                                                                               >  4:707087/1‑151 (MQ=255)
                                                                      gAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTc                                                                              <  1:320781/151‑1 (MQ=255)
                                                                        gCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCa                                                                             >  5:1662298/1‑150 (MQ=255)
                                                                         cacacaTCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAAt                                                                           >  5:208264/1‑151 (MQ=255)
                                                                          acacaTCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAAt                                                                           >  3:788292/1‑150 (MQ=255)
                                                                          acacaTCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATa                                                                          <  1:1051603/151‑1 (MQ=255)
                                                                             catcatCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCa                                                                       <  1:1348260/151‑1 (MQ=255)
                                                                              atcatcGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAg                                                                      <  1:1614247/151‑1 (MQ=255)
                                                                              atcatcGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAg                                                                      <  6:188993/151‑1 (MQ=255)
                                                                                catcGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGc                                                                     <  4:1349972/150‑1 (MQ=255)
                                                                                catcGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCg                                                                    <  3:1032433/151‑1 (MQ=255)
                                                                                 atcGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGa                                                                   <  1:433899/151‑1 (MQ=255)
                                                                                   ggTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAag                                                                 <  2:334957/150‑1 (MQ=255)
                                                                                       gCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAACACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAagtag                                                              <  6:315441/150‑1 (MQ=255)
                                                                                       gCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGGTCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAagtagt                                                             >  8:288569/1‑151 (MQ=255)
                                                                                             cAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCt                                                       <  4:1251429/151‑1 (MQ=255)
                                                                                              aaaCTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTc                                                      <  7:1331839/151‑1 (MQ=255)
                                                                                               aaCTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGGTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCACGCACGACCGGGCGCGTGAAGCGCGCGTGGGCAATACCAGCGAAGGAGTACAGcgcc                                                     >  7:588333/1‑148 (MQ=255)
                                                                                                actactTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCAc                                                    <  3:1555667/151‑1 (MQ=255)
                                                                                                  tactTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCAccc                                                  >  2:654217/1‑151 (MQ=255)
                                                                                                    ctTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCgg                                                >  4:153946/1‑151 (MQ=255)
                                                                                                      ttACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGt                                               <  7:1155343/150‑1 (MQ=255)
                                                                                                         ccATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGTCGCGTTTTTTCGAAAGTGACTTCCATTATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCGTCGTCAATACCAGCGAAGTAGTAAAGCTCACCCGGGTcc                                            >  5:649366/1‑150 (MQ=255)
                                                                                                         ccATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCCCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTcc                                            >  7:352482/1‑150 (MQ=255)
                                                                                                           aTCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGt                                         <  4:813624/151‑1 (MQ=255)
                                                                                                             cGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGtt                                        <  3:1118610/150‑1 (MQ=255)
                                                                                                              gACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGtttt                                      <  3:1363692/151‑1 (MQ=255)
                                                                                                               aCCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGAACGGGCGCTTGAAGCGCCCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCACTTTTc                                     >  5:275099/1‑151 (MQ=255)
                                                                                                                 cAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCTCTTTTTTTCTAAAGTGACTTCCATGATGATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCt                                   <  8:1215199/151‑1 (MQ=255)
                                                                                                                 cAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCTGGTTCCAGTTTTCCt                                   >  3:97924/1‑151 (MQ=255)
                                                                                                                 cAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCt                                   <  8:630076/151‑1 (MQ=255)
                                                                                                                  agagCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCt                                   >  1:903488/1‑150 (MQ=255)
                                                                                                                  agagCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTa                                  <  8:147140/151‑1 (MQ=255)
                                                                                                                     gCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGGTTTCCTACgg                               >  4:403177/1‑150 (MQ=255)
                                                                                                                      cAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCt                              >  4:986370/1‑151 (MQ=255)
                                                                                                                       aaCCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTAAGCtt                             >  8:465082/1‑151 (MQ=255)
                                                                                                                         ccccTTTAAAAGGGGTCAGGCAGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCtttt                           <  8:130553/151‑1 (MQ=255)
                                                                                                                         ccccTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCtttt                           >  3:458252/1‑151 (MQ=255)
                                                                                                                           ccTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTaa                         <  1:1217348/151‑1 (MQ=255)
                                                                                                                             tttAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTACGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTATAAAc                       >  2:238315/1‑151 (MQ=255)
                                                                                                                              ttAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGGTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAAc                       <  4:1063133/150‑1 (MQ=255)
                                                                                                                              ttAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTaaa                        <  2:612777/149‑1 (MQ=255)
                                                                                                                               tAAAACGGGTCAGGCCGCGGCGCGGTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCTGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGc                     >  8:709786/1‑151 (MQ=255)
                                                                                                                               tAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGc                     >  1:118375/1‑151 (MQ=255)
                                                                                                                               tAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGc                     >  8:112844/1‑151 (MQ=255)
                                                                                                                                  aaCGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGcc                    >  2:1639739/1‑149 (MQ=255)
                                                                                                                                  aaCGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGCCAg                  <  4:23555/151‑1 (MQ=255)
                                                                                                                                     gggTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGCCAGAAt               <  7:1675884/151‑1 (MQ=255)
                                                                                                                                            gCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGCCAGAATACCTGtt        <  6:120735/151‑1 (MQ=255)
                                                                                                                                             ccGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGCCAGAATACCTGTTg       <  8:1032676/151‑1 (MQ=255)
                                                                                                                                                 ggcgCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGCCAGAATACCTGTTGCCtg   >  5:339311/1‑151 (MQ=255)
                                                                                                                                                  gcgcGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGCCAGAATACCTGTTGCCtgt  <  7:447974/151‑1 (MQ=255)
                                                                                                                                                  gcgcGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGCCAGAATACCTGTTGCCtgt  <  6:1451708/151‑1 (MQ=255)
                                                                                                                                                        |                                                                                                                                                  
CCCTCTTCCACAATGGCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGCCAGAATACCTGTTGCCTGT  >  DH1/3668769‑3669064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: