Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1102,6180GC23.7% 615.3 / 55.9 236intergenic (‑70/+128)trmL/lldDtRNA (cytosine(34)‑2'‑O)‑methyltransferase TrmL/alpha‑hydroxy‑acid oxidizing enzyme
Reads supporting (aligned to +/- strand):  ref base G (27/153);  new base C (56/0);  total (83/153)
Fisher's exact test for biased strand distribution p-value = 5.79e-34
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 245 total aligned reads displayed.

AAAGCCGGTATTAGCGCAAAGACGGATGATGTTGCCAGTATTTGGCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGCGGGGGCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTTTCCCCTCACCCCGGCCCTCTCCCCGGAGGGGCGAGGGA‑GAAAAAAAGAGTGCGGTGTCGTTTCAGAGTGAGGGAGTACATACAGCGCCGAACGGTCCCCTCTCCCTGAGGGAGAGGGTTAGGGTGAGGGGGCGCAAACGACTATGCCGCATTCCCTTTCGCC  >  DH1/102468‑102767
                                                                                                                                                      |                                                                                                                                                      
aaaGCCGGTATTAGCGCAAAGACGGATGATGTTGCCAGTCTTTGGCGGAATTTCTTGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGCGGGGGCAGGATAGCAGAAAATTGGTGAGCAGGGCGGCCTTTTCCCCTCACCCCg                                                                                                                                                        <  2:340885/151‑1 (MQ=255)
aaaGCCGGTATTAGCGCAAAGACGGATGATGTTGCCAGTATTTGGCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGCGGGGGCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTTTCCCCTCACCCCg                                                                                                                                                        <  2:470611/151‑1 (MQ=255)
 aaGCCGGTATTAGCGCAAAGACGGATGATGTTGCCAGTATTTGGCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGCGGGGGCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTTTCCCCTCACCCCgg                                                                                                                                                       <  2:1389057/151‑1 (MQ=255)
  aGCCGGTATTAGCGCAAAGACGGATGATGTTGCCAGTATTTGGCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGCGGGGGCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTGTGCCCACCCCCCCGc                                                                                                                                                      >  2:1115545/1‑151 (MQ=255)
   gCCGGTATTAGCGCAAAGACGGATGATGTTGCCAGTATTTGGCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGCGGGGGCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTTTCCCCTCACCCCGGcc                                                                                                                                                     <  1:1426880/151‑1 (MQ=255)
    ccGGTATTAGCGCAAAGACGGATGATGTTGCCAGTATTTGGCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTATTGCGGGGGCAGGATAGCAGAAAATTGTTGCGCAGTGGGGCGTTGTGCCCCCCCCCCCGccc                                                                                                                                                    >  4:1598525/1‑151 (MQ=255)
     cGGTATTAGCGCAAAGACGGATGATGTTGCCAGTATTTGGCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGCGGGGGCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTGTAACCACACCCCCGcccc                                                                                                                                                   >  8:1072374/1‑150 (MQ=255)
        tATTAGCGCAAAGACGGATGATGTTGCCAGTATTTGGCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGCGGGGGCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTTTGCCCCCCCCCCCGCCCactc                                                                                                                                                >  5:814642/1‑151 (MQ=255)
        tATTAGCGCAAAGACGGATGATGTTGCCAGTATTTGGCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGCGGGGGCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTTTCGCCGCACCCCCGcccccac                                                                                                                                                >  5:203477/1‑147 (MQ=255)
         agtAGCGCAAAGACGGATGAGGTTGCCAGTATTTGGCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGCGGGGGCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTTTCCCCTCACCCCGGCCCTCTcc                                                                                                                                               <  1:1465/149‑1 (MQ=255)
         aTTAGCGCAAAGACGGATGATGTTGCCAGTATTTGGCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGCGGGGGCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTTTCCCCTCACCCCGGCCCtctc                                                                                                                                                <  7:77754/150‑1 (MQ=255)
            aGCGCTAAGACGGATGATGTTGCCAGTATTTGGCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGCGGTGGCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTTTCCCCTCACCCCGGCCCTCTccgc                                                                                                                                             <  4:218963/150‑3 (MQ=255)
               gcAAAGACGGATGATGTTGCCAGTATTTGGCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGCGGGGGCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTTTGGCCGCACCCCCGCCCACACCCCgcac                                                                                                                                         >  5:665308/1‑148 (MQ=255)
                cAAAGACGGATGATGTTGCCAGTATTTGGCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGCGGGGGCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTTTCCCCTCACCCCGGCCCTCTCCCCGGAgg                                                                                                                                        <  8:948791/151‑1 (MQ=255)
                cAAAGACGGATGATGTTGCCAGTATTTGGCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGCGGGGGCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTGTGGCCGGCCCCCGGCCCGCGCCCcggcgg                                                                                                                                        >  6:1316339/1‑151 (MQ=255)
                 aaaGACGGATGATGTTGCCAGTATTTGGCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGCGGGGGCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTTTCCCCTAACCCCGGCCCTCTCCCCGGAggg                                                                                                                                       >  3:1424782/1‑151 (MQ=255)
                 aaaGACGGATGATGTTGCCAGTATTTGGCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGCGGGGGCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTGTGGCCGCCCCCCCCCCCCCTccccccccgg                                                                                                                                       >  3:340102/1‑145 (MQ=255)
                 aaaGACGGATGATGTTGCCAGTATTTGGCGGAATTTCTGGTTCGTAAAGTACGATGTGTAGCATACTGCCCCCTTAGTGCGGGGGCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTTTCCCCTCACCCCGGCCCTCTCCCCGGAggg                                                                                                                                       <  6:538278/151‑1 (MQ=255)
                   aGACGGATGATGTTGCCAGTATTTGGCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGCGGGGGCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTTTGCCCGCCCCCCGGCCCGCGcccccccggggg                                                                                                                                     >  5:944360/1‑143 (MQ=255)
                   aGACGGATGATGTTGCCAGTATTTGGCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGCGGGGGCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTTTCCCCTCACCCCGGCCCTCTCCCCGGAGGGGc                                                                                                                                     <  2:585262/151‑1 (MQ=255)
                    gACGGATGATGTTGCCAGTATTTGGCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGCGGGGGCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTTTCCCCTCACCCCGGCCCTCTCCCCGGAGGGGCg                                                                                                                                    <  3:1165428/151‑1 (MQ=255)
                     aCGGATGATGTTGCCAGTATTTGGCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGCGGGGGCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTTTGGCCGCACCCCCGCCCAcccccccccgcggggc                                                                                                                                   >  3:760994/1‑141 (MQ=255)
                     aCGGATGATGTTGCCAGTATTTGGCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGCGGGGGCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTTTCCCCTCACCCCGGCCCTCTCCCCGGAGGGGCGa                                                                                                                                   <  3:124900/151‑1 (MQ=255)
                     aCGGATGATGTTGCCAGTATTTGGCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGCGGGGGCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTTTCCCCTCACCCCGGCCCTCTCCCCGGAGGGGCGa                                                                                                                                   <  5:328560/151‑1 (MQ=255)
                     aCGGATGATGTTGCCAGTATTTGGCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGCGGGGGCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTGTGCCCGCACACCCCCCCGCGcccccccgcggggc                                                                                                                                   >  4:1142392/1‑141 (MQ=255)
                        gatgatGTTGCCAGTATTTGGCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGCGGGGGCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTTTGCCCGCCCCCCCGCCCACGACCCCGCGGGGCGgggg                                                                                                                                >  4:397190/1‑151 (MQ=255)
                        gatgatGTTGCCAGTATTTGGCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGCGGGGGCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTTTCCCCTCACCCCGGCCCTCTCCCCGGAGGGGCGAggg                                                                                                                                <  5:436795/151‑1 (MQ=255)
                        gatgatGTTGCCAGTATTTGGCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGCGGGGGCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTTTCCCCTCACCCCGGCCCTCTCCCCGGAGGGGCGAggg                                                                                                                                <  6:1483173/151‑1 (MQ=255)
                        gatgatGTTGCCAGTATTTGGCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGCGGGGGCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTGTGGCCACACCCCCGCCCGCACCCCCGCCGGGCGgggg                                                                                                                                >  8:1458218/1‑151 (MQ=255)
                            atgTTGCCAGTATTTGGCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGCGGGGGCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTTTCCCCTCACCCCGGCCCTCTCCCCGGAGGGGCGAGGGA‑Gaa                                                                                                                           <  8:393033/151‑1 (MQ=255)
                              gTTGCCAGTATTTGGCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGCGGGGGCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTTTCCCCTCACCCCGGCCCTCTCCCCGGAGGGGCGAGGGA‑Gaaaa                                                                                                                         <  6:1456807/151‑1 (MQ=255)
                              gTTGCCAGTATTTGGCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGCGGGGGCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTTTACCCTCACCCCGGCCCTCTCCCCCCCGGGGCGAGGTA‑Gaaa                                                                                                                          >  8:529051/1‑150 (MQ=255)
                               ttGCCAGTATTTGGCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGCGGGGGCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTTTCCCCTCACCCCGGCCCTCTCCCCGGAGGGGCGAGGGA‑Gaaaa                                                                                                                         <  1:1342634/150‑1 (MQ=255)
                                 gCCAGTATTTGGCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGCGGGGGCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTTTGGCCGCACCCCCGCCCCCACCCCCGCCGGGCgcgcgcggcaaaa                                                                                                                        >  4:1179717/1‑138 (MQ=255)
                                  ccAGTATTTGGCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGCGGGGGCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTTTCCCCTCACCCCGGCCCTCTCCCCGGAGGGGCGAGGGA‑GAAAAAAag                                                                                                                     <  3:1114894/151‑1 (MQ=255)
                                    aGTATTTGGCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGCGGGGGCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTTTCCCCTCACCCCGGCCCTCTCCCCGGAGGGGCGAGGGA‑GAAAAAAagag                                                                                                                   <  1:3099/151‑1 (MQ=255)
                                    aGTATTTGGCGGAATTTCTGGTGCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGCGGGGGCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTTTCCCCTCACCCCGGCCCTCTCCCCGGAGGGGCGAGGGA‑GAAAAAAagag                                                                                                                   <  5:173143/151‑1 (MQ=255)
                                       aTTTGGCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGCGGGGGCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTTTCCCCTCACCCCGGCCCTCTCCCCGGAGGGGCGAGGGA‑GAAAAAAAGAGTGc                                                                                                                <  1:598260/151‑1 (MQ=255)
                                        tttGGCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGCGGGGGCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTTTGCCCACACCCCCGCCCACTCCCCCGAGGGGCGCGGGC‑GAAAAAAagaggggg                                                                                                               >  2:1530563/1‑147 (MQ=255)
                                           ggCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGCGGGGGCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTGTGCCCCGGCCCCCGCCCCCGGCCCCGAGGGGCGCGGGC‑GGAAAAAaaagggggggg                                                                                                            >  4:760843/1‑144 (MQ=255)
                                              ggAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGCGGGGGCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTTTCCCCTCACCCCGGCCCTCTCCCCGGAGGGGCGAGGGA‑GAAAAAAAGAGTGCGGTGTCg                                                                                                         <  3:1061976/151‑1 (MQ=255)
                                               gAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCACCCTTAGTGCGGGGGCAGGATAGCAGAAAATTGGTGAGCAGTGCTGCCTTTTCCCCTCACCCCGGCCCTCTCCCCGGAGGGGCGAGGGA‑GAAAAAAAGAGTGCGGTTTc                                                                                                          <  8:1565903/149‑1 (MQ=255)
                                                   ttCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGCGGGGGCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTTTCCCCTCACCCCGGCCCTCTCCCCGGAGGGGCGAGGGA‑GAAAAAAAGAGTGCGGTGTCGTTTc                                                                                                     <  7:984132/150‑1 (MQ=255)
                                                    tCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGCGGGGGCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTTTCCCCTCACCCCGGCCCTCTCCCCGGAGGGGCGAGGGA‑GAAAAAAAGAGTGCGGTGTCGTTTCa                                                                                                    <  1:648154/150‑1 (MQ=255)
                                                      tGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGCGGGGGCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTTTGGCCCCCCCCCCGCCCCCCCCCCCGCCGGGGGGGGGG‑GCAAAAAACAGTGCgggggggggttag                                                                                                   >  5:524197/1‑138 (MQ=255)
                                                       ggTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGCGGGGGCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTTTCCCCTCACCCCGGCCCTCTCCCCGGAGGGGCGAGGGA‑GAAAAAAAGAGTGCGGTGTCGTTTCAGAGt                                                                                                <  3:1450501/151‑1 (MQ=255)
                                                         ttCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGCGGGGGCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTTTGCCCGACCCACCCCCCGCTCCCCGGAGGGGGGGGGGG‑GAAAAAAAAAGTGCGGGGTGGGTTTagagagg                                                                                              >  2:555887/1‑148 (MQ=255)
                                                           ggTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGCGGGGGCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTTTCCCCTCACCCCGGCCCTCTCCCCGGAGGGGCGAGGGA‑GAAAAAAAGAGTGCGGTGTCGTTTCAGAGTGAgg                                                                                            <  8:727107/150‑1 (MQ=255)
                                                           cGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGCGGGGGCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTTTCCCCTCACCCCGGCCCTCTCCCCGGAGGGGCGAGGGA‑GAAAAAAAGAGTGCGGTGTCGTTTCAGAGTGAg                                                                                             <  5:1412293/150‑1 (MQ=255)
                                                            gTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGCGGGGGCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTGTGCCCGCCCCCCCGCCCCCCCCCCCGCGGGGGGCGGGC‑GCAAAAAAAAGTGCGGGGGGGGTTCTGGGTGAggg                                                                                           >  5:1556082/1‑151 (MQ=255)
                                                            gTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGCGGGGGCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTGTACCCGCACCCCGGCCCGCGCCCCGGCGGGGGGCGGGG‑GGAAAAAATACAGGGGTGTGGGTTGAGCGTGAggg                                                                                           >  7:1675855/1‑151 (MQ=255)
                                                              aaaGTACGATGTTTAGCATACTGCCCCCTTAGTGCGGGGGCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTTTCCCCTCACCCCGGCCCTCTCCCCGGAGGGGCGAGGGA‑GAAAAAAAGAGTGCGGTGTCGTTTCAGAGTGAGGGAg                                                                                         <  5:79572/151‑1 (MQ=255)
                                                               aaGTACGATGTTTAGCATACTGCCCCCTTAGTGCGGGGGCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTTTCCCCTCACCCCGGCCCTCTCCCCGGAGGGGCGAGGGA‑GAAAAAAAGAGTGCGGTGTCGTTTCAGAGTGAGGGAGt                                                                                        <  1:220848/151‑1 (MQ=255)
                                                               aaGTACGATGTTTAGCATACTGCCCCCTTAGTGCGGGGGCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTTTCCCCTCACCCCGGCCCTCTCCCCGGAGGGGCGAGGGA‑GAAAAAAAGAGTGCGGTGTCGTTTCAGAGTGAGGGAGt                                                                                        <  8:1028373/151‑1 (MQ=255)
                                                                  tACGATGTTTAGCATACTGCCCCCTTAGTGCGGGGGCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTTTGCCCCAACCCCCGCCCACTCCCCCGAGGGGCGAGGGA‑GAAAAAAATAATGCGGGGTCGTTTGAGAGTGTgggcggtc                                                                                      >  4:1451430/1‑145 (MQ=255)
                                                                    cGATGTTTAGCATACTGCCCCCTTAGTGCGGGGGCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTTTAAACGAAACACCGCCCAAAAAACCCACGGGCGAGGGA‑GAAAAAAAAAGTGGGGTGTGGGTTGAGAGGGGGGTAGttcat                                                                                    >  2:1204889/1‑150 (MQ=255)
                                                                     gATGTTTAGCATACTGCCCCCTTAGTGCGGGGGCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTTTCCCCTCACCCCGGCCCTCTCCCCGGAGGGGCGAGGGA‑GAAAAAAAGAGTGCGGTGTCGTTTCAGAGTGAGGGAGtacatac                                                                                  <  6:327750/151‑1 (MQ=255)
                                                                      aTGTTTAGCATACTGCCCCCTTAGTGCGGGGGCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTTTCCCCTCACCCCGGCCCTCTCCCCGGAGGGGCGAGGGA‑GAAAAAAAGAGTGCGGTGTCGTTTCAGAGTGAGGGAGtacataca                                                                                 <  8:429769/151‑1 (MQ=255)
                                                                        gTTTAGCATACTGCCCCCTTAGTGCGGGGGCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTGTCCCCGCACCCCCCCCCGCGCCCCCGACGGGCGCCGGACAAAAAAAAGACGGCGGGGGCGGTGCAGAGTGAGGGAGtataaacac                                                                                >  2:81470/1‑150 (MQ=255)
                                                                              cATACTGCCCCCTTAGTGCGGGGGCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTTTCCCCTCACCCCGGCCCTCTCCCCGGAGGGGCGAGGGA‑GAAAAAAAGAGTGCGGTGTCGTTTCAGAGTGAGGGAGTACATACAGCGCCGaa                                                                         <  1:1141029/151‑1 (MQ=255)
                                                                               aTACTGCCCCCTTAGTGCGGGGGCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTTTCCCCTCACCCCGGCCCTCTCCCCGGAGGGGCGAGGGA‑GAAAAAAAGAGTGCGGTGTCGTTTCAGAGTGAGGGAGTACATACAGCGCCGAAc                                                                        <  4:80502/151‑1 (MQ=255)
                                                                                 aCTGCCCCCTTAGTGCGGGGGCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTTTCCCCTCACCCCGGCCCTCTCCCCGGAGGGGCGAGGGA‑GAAAAAAAGAGTGCGGTGTCGTTTCAGAGTGAGGGAGTACATACAGCGCCGAACgg                                                                      <  3:1373234/151‑1 (MQ=255)
                                                                                 aCTGCCCCCTTAGTGCGGGGGCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTTTCCCCTCACCCCGGCCCTCTCCCCGGAGGGGCGAGGGA‑GAAAAAAAGAGTGCGGTGTCGTTTCAGAGTGAGGGAGTACATACAGCGCCGAACg                                                                       <  6:173438/150‑1 (MQ=255)
                                                                                  cTGCCCCCTTAGTGCGGGGGCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTGTGACCACACCCCCGCCCCCGCCCCGGAGGGGGGAGGGA‑GAAAAAAAAAGAGGCGGGTGGGTTGCGACTGAGGGGGTACGGACAGCGccccaagct                                                                     >  6:851949/1‑144 (MQ=255)
                                                                                     cccccTTAGTGCGGGGGCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTTTCCCCTCACCCCGGCCCTCTCCCCGGAGGGGCGAGGGA‑GAAAAAAAGAGTGCGGTGTCGTTTCAGAGTGAGGGAGTACATACAGCGCCGAACGGTccc                                                                  <  7:184435/151‑1 (MQ=255)
                                                                                        ccTTAGTGCGGGGGCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTTTCCCCTCACCCCGGCCCTCTCCCCGGAGGGGCGAGGGA‑GAAAAAAAGAGTGCGGTGTCGTTTCAGAGTGAGGGAGTACATACAGCGCCGAACGGTCCCctc                                                               <  2:38554/151‑1 (MQ=255)
                                                                                           tAGTGCGGGGGCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTTTCCCCTCACCCCGGCCCTCTCCCCGGAGGGGCGAGGGA‑GAAAAAAAGAGTGCGGTGTCGTTTCAGAGTGAGGGAGTACATACAGCGCCGAACGGTCCCCtctc                                                             <  5:1221119/150‑1 (MQ=255)
                                                                                            aGTGCGGGGGCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTTTCCCCTCACCCCGGCCCTCTCCCCGGAGGGGCGAGGGA‑GAAAAAAAGAGTGCGGTGTCGTTTCAGAGTGAGGGAGTACATACAGCGCCGaa                                                                         <  4:1306312/137‑1 (MQ=255)
                                                                                             gTGCGGGGGCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTTTCCCCTCACCCCGGCCCTCTCCCCGGAGGGGCGAGGGA‑GAAAAAAAGAGTGCGGTGTCGTTTCAGAGTGAGGGAGTACATACAGCGCCGAACGGTCCCCTCTCCCt                                                          <  3:230094/151‑1 (MQ=255)
                                                                                               gCGGGGGCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTTTCCCCTCACCCCGGCCCTCTCCCCGGAGGGGCGAGGGA‑GAAAAAAGGAGTGCGGTGTCGTTTCAGAGTGAGGGAGTACATACAGCGCCGAACGGTCCCCTCTCCCTGa                                                        <  5:1373359/151‑1 (MQ=255)
                                                                                                cGGGGGCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTTTCGCCACCCCCCCGCCCACACCCCCGGGGGGCGGGGGG‑GAAAAAAACAGGGGGGGGTGGGTTGACAGTGTGGGCGGACATACAGCGGCGCACGGGCCCCTCAcccggcg                                                       >  4:1524681/1‑147 (MQ=255)
                                                                                                cGGGGGCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTTTCCCCTCACCCCGGCCCTCTCCCCGGAGGGGCGAGGGA‑GAAAAAAAGAGTGCGGTGTCGTTTCAGAGTGAGGGAGTACATACAGCGCCGAACGGTCCCCTCTCCCTGAg                                                       <  5:713579/151‑1 (MQ=255)
                                                                                                cGGGGGCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTTTCCCCTCACCCCGGCCCTCTCCCCGGAGGGGCGAGGGA‑GAAAAAAAGAGTGCGGTGTCGTTTCAGAGTGAGGGAGTACATACAGCGCCGAACGGTCCCCTCTCCCTGAg                                                       <  1:1312232/151‑1 (MQ=255)
                                                                                                cGGGGGCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTTTCCCCTCACCCCGGCCCTCTCCCCGGAGGGGCGAGGGA‑GAAAAAAAGAGTGCGGTGTCGTTTCAGAGTGAGGGAGTACATACAGCGCCGAACGGTCCCCTCTCCCTGAg                                                       <  5:1250134/151‑1 (MQ=255)
                                                                                                    ggCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTTTCCCCTCACCCCGGCCCTCTCCCCGGAGGGGCGAGGGA‑GAAAAAAAGAGTGCGGTGTCGTTTCAGAGTGAGGGAGTACATACAGCGCCGAACGGTCCCCTCTCCCTGAGGgag                                                   <  7:961549/151‑1 (MQ=255)
                                                                                                      cAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTTTGCCCTCCCCCCCGCCCACTCCCCCGCGGGGGGGGGGC‑GCAAAAAAGAGGGGGGGGGGGGTTGAGAGTGAGGGGGTTGATACCGCGCCGCACCGGGCCCGCGCCCGGAgggcggg                                                 >  6:158933/1‑147 (MQ=255)
                                                                                                         gATAGCAGAAAATTCGTGAGCAGTGCGGCCTTTTCCCCTCACCCCGGCCCGCTCCCCGGTGGGGCGAGGGA‑GAAAAAAAGAGTGCGGTGTCGTTTCAGAGTGAGGGAGTACATTCAGCGCCGAAGGGTCCCCTCTCCCTGAGGGAGAGGGt                                              <  4:946944/151‑1 (MQ=255)
                                                                                                              cAGAAAATTGGTGAGCAGTGCGGCCTTTTCCCCTCACCCCGGCCCTCTCCCCGGAGGGGCGAGGGA‑GAAAAAAAGAGTGCGGTGTCGTTTCAGAGTGAGGGAGTACATACAGCGCCGAACGGTCCCCTCTCCCTGAGGGAGAGGGTTAggg                                         <  6:647072/151‑1 (MQ=255)
                                                                                                              cAGAAAATTGGTGAGCAGTGCGGCCTTTTCCCCTCACCCCGGCCCTCTCCCCGGAGGGGCGAGGGA‑GAAAAAAAGAGTGCGGTGTCGTTTCAGAGTGAGGGAGTACATACAGCGCCGAACGGTCCCCTCTCCCTGAGGGAGAGGGTTAggg                                         <  4:898031/151‑1 (MQ=255)
                                                                                                               aGAAAATTGGTGAGCAGTGCGGCCTTTTCCCCTCACCCCGGCCCTCTCCCCGGAGGGGCGAGGGA‑GAAAAAAAGAGTGCGGTGTCGTTTCAGAGTGAGGGAGTACATACAGCGCCGAACGGTCCCCTCTCCCTGAGGGAGAGGGTTAGGGt                                        <  3:828362/151‑1 (MQ=255)
                                                                                                                gAAAATTGGTGAGCAGTGCGGCCTTTTACCCAAACCCCCGCCCACAACCCCCAGGGGCGACGGA‑GAAAAAAAAAGTGGGGTGTGGTTTGAGAGTGAGGGATTAAAAAAAAACCCCAAACGTCCCCCCTCCCAGAGGGACAGCGtgtggggg                                       >  4:365627/1‑144 (MQ=255)
                                                                                                                      tGGTGAGCAGTGCGGCCTTTTCCCCTCACCCCGGCCCTCTCCCCGGAGGGGCGAGGGA‑GAAAAAAAGAGTGCGGTGTCGTTTCAGAGTGAGGGAGTACATACAGCGCCGAACGGTCCCCTCTCCCTGAGGGAGAGGGTTAGGGTGAgggg                                  <  3:441078/150‑1 (MQ=255)
                                                                                                                      tGGTGAGCAGTGCGGCCTTTTCCCCTCACCCCGGCCCTCTCCCCGGAGGGGCGAGGGA‑GAAAAAAAGAGTGCGGTGGCGTTTCAGAGTGAGGGAGTACATACAGCGCCGAACGGTCCCCTCTCCCTGAGGGAGAGGGTTAGGGTGAgggg                                  <  3:1599787/150‑1 (MQ=255)
                                                                                                                            gCAGTGCGGTCTTTTCCCCTCACCCCGGCCCTCTCCCCGGAGGGGCGAGGGA‑GAAAAAAAGAGTGCGGGGTCGTTTCAGAGTGAGGGAGTACATACAGCGCCGAACGGTCCCCTCTCCCTGAGGGAGAGGGTTAGGGTGAGGGGGCGCaaa                           <  6:732675/151‑1 (MQ=255)
                                                                                                                                       ttttCCCCTCACCCCGGCCCTCTCCCCGGAGGGGCGAGGGA‑GAAAAAAAGAGTGCGGTGTCGTTTCAGAGTGAGGGAGTACATACAGCGCCGAACGGTCCCCTCTCCCTGAGGGAGAGGGTTAGGGTGAGGGGGCGCACGCGACTCTGCCg                >  1:1576235/1‑151 (MQ=255)
                                                                                                                                       ttttCCCCTCACCCCGGCCCTCTCCCCGGAGGGGCGAGGGA‑GAAAAAAAGAGTGCGGTGTCGTTTCAGAGTGAGGGAGTACATACAGCGCCGAACGGTCCCCTCTCCCTGAGGGAGAGGGTTAGGGTGAGGGGGCGCAAACGACTATGCCg                >  5:1658438/1‑151 (MQ=255)
                                                                                                                                        tttCCCCTCACCCCGGCCCTCTCCCCGGAGGGGCGAGGGA‑GAAAAAAAGAGTGCGGTGTCGTTTCAGAGTGAGGGAGTACATACAGCGCCGAACGGTCCCCTCTCCCTGAGGGAGAGGGTTTGGGTGGGGGGGCGCGAACGCCTATGCCGc               >  1:827199/1‑151 (MQ=255)
                                                                                                                                        tttCCCCTCACCCCGGCCCTCTCCCCGGAGGGGCGAGGGA‑GAAAAAAAGAGTGCGGTGTCGTTTCAGAGAGAGGGAGTACATAACGAGAGGAACGGTCCCCTCTACCTGAGGGAGAGGGTTAGGGTGAGGGGGCGGAAACGAGTATGCCGc               >  4:1001779/1‑151 (MQ=255)
                                                                                                                                         ttCCCCTCCCCCCGCCCCTCTCCCCGGCGGGGCGAGGGA‑CAAAAAACCCGGGCGGGGTCGTTTCACTGTCCGGGAGCACATCCAGCCCCCAACGGTCCCCTCTCCCTGCGGGAGAGGGTTCGGGTGAGGGGGCGCAAACGACTATGCCGc               <  2:870897/150‑1 (MQ=255)
                                                                                                                                         ttCCCCTACCCCGGGCCGTCTCCCGGGTGGGGGGTGGTA‑TAAAAAATTTGTGGGGTGTGGTTTCAGATTTTGGTAGTACATACTGGGCGGAAGGGTCCCCTGTCCTTGAGGGTTTGGGTTAGGGTGTGGGGGCGCAAACGACTATGCCGc               <  4:667569/150‑1 (MQ=255)
                                                                                                                                         tgccccTCACCCCGGCCCTCTCCCGGGTGGGGCGTGGGA‑GAAAAAAAGAGTGCGGTGTGGTTTCAGAGTTAGGTCTTACATACAGCGCCGAACGGTCCCGTCTCCCTGAGGTAGTGGTTTAGGGTGCGGGGGCGCAAACGACTATGCCGc               <  1:401865/148‑1 (MQ=255)
                                                                                                                                             ccTCACCCCGGCCCTCTCCCCGGAGGGGCGAGGGA‑GAAAAAAAGAGTGCGGTGTCGTTTCAGAGTGAGGGAGTACATACAGCGCCGCACGGTCCCCTCTCCCTGAGGGAGAGGGTTAGGGTGAGGGGGCGCCAACGCCGATGCCGCCTTcc          >  7:1568439/1‑151 (MQ=255)
                                                                                                                                                cACCCCGGCCCTCTCCCCGGAGGGGCGAGGGA‑GAAAAAAAGAGTGCGGTGTCGTTTCAGAGTGAGGGAGTACATACAGCGCCGAACGGTCCCCTCTCGCGGAGGGAGAGGGGTAGGGTGAGGGGGCGCAAACGACTATGCCGGATTCCCtt       >  7:1236578/1‑151 (MQ=255)
                                                                                                                                                cACCCCGGCCCTCTCCCCGGAGGGGCGAGGGA‑GAAAAAAAGAGTGCGGTGTCGTTTCAGAGTGAGGGAGTACATACAGCGCCGAACGGTCCCCTCTCCCTGAGGGAGAGGGTTAGGGTGAGGGGGCGCAAACGCCTATGCCGCATTcccgt       >  2:170888/1‑149 (MQ=255)
                                                                                                                                                 cccccGGCCCTCTCCCCGGAGGGGCGAGGGA‑GAAAAACCGTGTGCGGTGTCGTTTCACCGTGCGGGAGTCAATACCGCGCCGAACGGTCCCCTCTCCCTGAGGGAGTGGGTTCGGGTGCGGGGGCGCAAACGACTATGCCGCATTCCCttt      <  6:115955/150‑1 (MQ=255)
                                                                                                                                                 cccccGGCCCTCTCCCCGGAGGGGCGAGGGA‑GAAAAAAAGAGTGCGGTGTCGTTTCAGAGTGAGGGAGTACATACAGCGCCGAACGGTCCCCCCTCCCTGAGGGAGAGGGTTAGGGTGAGGGGGCGCAAACGACTATGCCGCATTCCCttt      <  3:939589/150‑1 (MQ=255)
                                                                                                                                                 aCCCCGGCCCTCTCCCCGGCTGGGCGAGGTA‑GAAAAAAAGATGGCGGTGTCGTTTCATAGTGAGGGATTACATAAAGCGCCGAACGGTCCCCTCGCCCTGAGGGAGTGGGTTAGGTGGAGGGGGCGCAAACGACTATGCCGCATTCCCttt      <  6:555198/151‑1 (MQ=255)
                                                                                                                                                     cGGCCCTCTCCCCGGAGGGGCGAGGGA‑GAAAAAAAGAGTGCGGTGTCGTTTCAGAGTGAGGGAGTACATACAGCGCCGAACGGTCCCCTCTCCCTGAGGGAGAGGGTTAGGGTGAGGGGGCGCAAACGACTATGCCGCATTCCCTTTCGcc  >  2:1340180/1‑151 (MQ=255)
                                                                                                                                                     cGGCCCTCTCCCCGGAGGGGCGAGGGA‑GAAAAAAAGAGTGCGGTGTCGTTTCAGAGTGAGGGAGTACATACAGCGCCGAACGGTCCCCTCTCCCTGAGGGAGAGGGTTAGGGTGAGGGGGCGCAAACGACTATGCCGCATTCCCTTTCGcc  >  8:558225/1‑151 (MQ=255)
                                                                                                                                                      ggCCCTCTCCCCGGAGGGGCGAGGGA‑GAAAAAAAGAGTGCGGTGTCGTTTCAGAGTGAGGGAGTACATACAGCGCCGAACGGTCCCCTCTCCCTGAGGGAGAGGGTTAGGGTGAGGGGGCGCAAACGCCTATGCCGCATTCCCTTTCGcc  >  6:1248715/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                      
AAAGCCGGTATTAGCGCAAAGACGGATGATGTTGCCAGTATTTGGCGGAATTTCTGGTTCGTAAAGTACGATGTTTAGCATACTGCCCCCTTAGTGCGGGGGCAGGATAGCAGAAAATTGGTGAGCAGTGCGGCCTTTTCCCCTCACCCCGGCCCTCTCCCCGGAGGGGCGAGGGA‑GAAAAAAAGAGTGCGGTGTCGTTTCAGAGTGAGGGAGTACATACAGCGCCGAACGGTCCCCTCTCCCTGAGGGAGAGGGTTAGGGTGAGGGGGCGCAAACGACTATGCCGCATTCCCTTTCGCC  >  DH1/102468‑102767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑