Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1613,5970GC25.1% 281.1 / 42.1 234intergenic (+458/+565)garK/yhaCglycerate 2‑kinase/pentapeptide repeats‑related protein
Reads supporting (aligned to +/- strand):  ref base G (56/81);  new base C (1/45);  total (62/172)
Fisher's exact test for biased strand distribution p-value = 7.18e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 238 total aligned reads displayed.

CCGTACTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑GC‑TTCGGCGGG‑TTTTTGCTTTTGGAGGGGCAGAAAGATGAATGAC‑TGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAA  >  DH1/613449‑613745
                                                                                                                                                        |                                                                                                                                                     
ccGTACTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑GC‑TTCGGCGGG‑TTTTTGCTTTTGGAGGGGCAGaa                                                                                                                                                     <  8:85102/151‑1 (MQ=255)
ccGTACTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGAATTACGTAAAAACC‑C‑GA‑TACGTCGCG‑TGTTATCTATTGGAGGGGCAgc                                                                                                                                                      >  7:741244/1‑149 (MQ=38)
 cGTACTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑GC‑TTCGGCGGG‑TTTTTGCTTTTGGAGGGGCAGaa                                                                                                                                                     >  3:7486/1‑150 (MQ=255)
  gTACTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGAGATAACAAGCCATATCGGTAACATTCACCTCAGATACGTAAAAAAC‑C‑GC‑TTCGGCGGG‑GTTGTGCATTTAGAGCGGCAGaa                                                                                                                                                     >  7:1054437/1‑149 (MQ=255)
  gTACTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑GC‑TTCGGCGGG‑TTTTTGCTTTTGGAGGGgaagaa                                                                                                                                                     >  3:100469/1‑149 (MQ=255)
  gTACTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑GC‑TTCGGCGGG‑TTTTTGCTTTTGGAGGGGCAGaa                                                                                                                                                     >  8:675430/1‑149 (MQ=255)
  gTACTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑GC‑TTCGGCGGG‑TTTTTGCTTTTGGAGGGGCAGAAAg                                                                                                                                                   <  6:663112/151‑1 (MQ=255)
  gTACTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑GC‑TTCGGCGGG‑TTTTTGCTTTTGGAGGGGCAGAAAg                                                                                                                                                   <  1:686251/151‑1 (MQ=255)
     cTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGATTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑GC‑TTCGGCTGG‑TTTTTGGTTTTGGAGGGGCAGAAAGatg                                                                                                                                                >  5:796393/1‑151 (MQ=255)
      tGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑GC‑TTCGGCGGG‑TTTTTCCTTTTTGATGGGaagaaagaggt                                                                                                                                               >  2:469158/1‑148 (MQ=255)
        aagcggggTAGTCTGCTTGAGCCAGTGATCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACGCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑GC‑TTCGGCGGG‑TTTTTTCTTTTGGAGGGGCAGAAAGatgaat                                                                                                                                             <  2:110238/146‑1 (MQ=21)
        aaCCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑GC‑TTCGGCGGG‑TTTTTGCTTTTGGAGGGGCAGAAAGatgaat                                                                                                                                             <  5:1140956/151‑1 (MQ=255)
        aaCCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑GC‑TTCGGCGGG‑TTTTTGCTTTTGGAGGGGCAGAAAGatgaat                                                                                                                                             <  3:678347/151‑1 (MQ=255)
         agcggggttgtgtgctTGTGCCAGTGAGCGATTGCTGGCCTAGATTATTGACTGTACACCACAGAACCCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACC‑C‑GC‑TTCGGCGGG‑TTTTTACTTTTGGAGGGGCAGAAAGatgaatg                                                                                                                                            <  2:1037943/139‑1 (MQ=18)
         aCCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑GC‑TTCGGCGGG‑TTTTTACTTTTGGAGGGGCaaaatcagaag                                                                                                                                              >  4:748604/1‑142 (MQ=255)
          gcgctgttgtctggtTGAGCCAGTTAGAGATTGATGGACTAGATAAATGACTCTCCACTAAAGAACGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACC‑C‑GC‑TTCGGCGGG‑TTTTTACTTTTGGAGGGGCAGAAAGatgaatg                                                                                                                                            <  3:529443/140‑1 (MQ=14)
          cccGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑GC‑TTCGGCGGG‑TTTTTGCTTTTGGAGGGGCAGAAAGatgaatga                                                                                                                                           <  3:595798/151‑1 (MQ=255)
          cccGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑GC‑TTCGGCGGG‑TTTTTGCTTTTGGAGGGGCAGAAAGatgaatga                                                                                                                                           <  7:662087/151‑1 (MQ=255)
              gttattctgctTGAGGCAGTCACAGATTGATTGCCTAGATGACTCACTCTCCACCACAGAACGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACC‑C‑GC‑TTCGGCGGG‑TTTTTACTTTTGGAGGGGCAGAAAGATGAATGAC‑TGt                                                                                                                                      <  1:902564/145‑1 (MQ=14)
                 aGGCTGCTTGAGCCTGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑GC‑TTCGGCGGG‑TTTTTGCTTTTGGAGGGGCAGAAAGATGAATGAC‑TGTcc                                                                                                                                    <  2:1537358/150‑1 (MQ=255)
                 aGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAGTGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑GC‑TTCGGCGGG‑TTTTTGCTTTTGGAGGGGCAGAAAGATGAATGAC‑TGTcc                                                                                                                                    <  2:1000073/150‑1 (MQ=255)
                 aGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAAACCCCATAATCGATCAGTTAAACCTGAATTAACTAAAAACC‑A‑AA‑TTCTGAGCG‑TTTTTGATTTTGTAGGGGAAGAAAGATGAATGAA‑TGtaa                                                                                                                                    >  6:874121/1‑148 (MQ=255)
                  ggCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑GC‑TTCGGCGGG‑TTTTTGCTTTTGGAGGGGCAGAAAGATGAATGAC‑TGTCCac                                                                                                                                  <  7:1212543/151‑1 (MQ=255)
                   gctgctTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑GC‑TTCGGCGGG‑TTTTTGCTTTTGGAGGGGCAGAAAGATGAATGAC‑TGTCCacg                                                                                                                                 <  2:613067/151‑1 (MQ=255)
                   gctgctTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑GC‑TTCGGCGGG‑TTTTTGCTTTTGGAGGGGCAGAAAGATGAATGAC‑TGTCCacg                                                                                                                                 <  5:579993/151‑1 (MQ=255)
                     ggctTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑GC‑TTCGGCGGG‑TTTTTGCTTTTGGAGGGGCAGAAAGATGAATGAC‑TGTCCacgac                                                                                                                               <  8:234797/150‑1 (MQ=255)
                      gctTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCTGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑GC‑TTCGGCGGG‑TTTTTGCTTTTGGAGGGGCAGAAAGATGAATGAC‑TGTCCacgac                                                                                                                               <  2:1505374/150‑1 (MQ=255)
                      gctTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑GC‑TTCGGCGGG‑TTTTTGCTTTTGGAGGGGCAGAAAGATGAATGAC‑TGTCCacga                                                                                                                                <  7:586457/149‑1 (MQ=255)
                      gctTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTAACCTGATTTAAGTAAAAACC‑C‑TC‑TTCTACGGG‑TATTTAGTTTTGGATTGTCTGAAAGAAGAATGAC‑TGTCAacgaa                                                                                                                               >  8:439881/1‑149 (MQ=21)
                           cgCCAGTGATCTATTTCTGGCCTAGATTAATGACTCTCCACTACAGAACCCGGATTATGTGTAAGTTTCACCTGATTTACTTAAAAACC‑C‑GC‑TTCGGCGGT‑TTTTTGCTTTTGTAGGGGCAGAAATATGAATGAC‑TGTCCACGACGCtata                                                                                                                         <  6:1057193/150‑1 (MQ=255)
                            gCCAGTGAGCGATTGCTGGCCTAGATTAATGACTGTCCACGACAGAACCCGGCTTATGGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑GC‑TTCGGCGGT‑TTTTTGCTTTTGGAGGGGCAGAAAGATGAATGAC‑TGGCCACGACGCTATAc                                                                                                                        <  3:317489/151‑1 (MQ=255)
                            gCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCCGAATATAGGTCAGTTTCACCTGATTTAAGTAAAAAAC‑A‑GC‑TTCGTCGGC‑TTATTACTTTTTTAGGGGCAGAAAGATGAATGAC‑TGTcc                                                                                                                                    >  8:12424/1‑139 (MQ=21)
                              cAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACACGGCTTATCGGTCACTTTCACCTGATTTAAGTAAAAAAC‑C‑TA‑TTCGGAGGG‑GTTTTGCTTTTGGAGGTGCAGAAAGATGAATGAG‑TGTCCACGACGGTAGAccc                                                                                                                      >  7:1368954/1‑151 (MQ=21)
                               aGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACACAACCCGGCTTATAGGTCAGTTTAACCTGATTTACGTAAAAACC‑C‑GC‑TTCGGATGG‑TTATTTCTTTTGGAGGGGCAGAAAGATGAATCAA‑TGTCCACGACACTATAccc                                                                                                                      >  4:451211/1‑150 (MQ=18)
                                 ggAGCGATTGCTGGCCTAGATGAATGACTGGCCACGCCAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACTTAAAAACC‑C‑GC‑TTCGGCGGG‑TTTTTGCTTTTGGAGGGGCAGAAAGATGAATGAC‑TGTCCACGACGCTATACCCaaa                                                                                                                   <  1:332476/150‑1 (MQ=255)
                                     cGATTGCTGGCCTAGATGAATGACTGTCCACGACACAAAACCCATAATCAATCACTTTCACCTGATTTACGTAAAAACA‑A‑AC‑TTAGTCCGG‑ATATTTCTTTAGGAGGGGCAGAAAGATGAATGAA‑TGTAAAAGACACTATACCCAaaaga                                                                                                                >  2:457471/1‑150 (MQ=12)
                                        ttGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGAAAAAACA‑C‑GC‑GACCGCGGC‑TGATTGCTATTCGCCGGGCCGTAACAGGTATGCCTTGGCCA‑GACGCTAAAACCAAAAGAAAGc                                                                                                            >  7:296693/1‑151 (MQ=255)
                                        ttGCTGGCCTAGATGAATGACTGTCCACCACAGAACCCGGCTTAGCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑GC‑TTCGGCGGG‑TTTTTGCTTTTGGAGGGGCAGAAAGATGAATGAC‑TGTCCACGACGCTATACCCAaaagaaag                                                                                                             <  6:1382233/150‑1 (MQ=255)
                                             ggCCTAGATTATTCCCTGTCCACTACAGAACCCGGCTTATCGGTCAGTTTCACCTTATTTACTTAAAAACC‑C‑GC‑TGGGGGGGT‑TTTTTGGTTTTGGTGGGGCAGAAAGATTAATGAC‑TGGCCACGACGCTATACCCAAAAGAAAGCGGCtt                                                                                                       <  5:460580/151‑1 (MQ=14)
                                             ggCCTAGATGAATGACTGCCCACCACAGAACCCGGCTTAGCGGTCTGTTTCACCTTATTTACGTAAAACCC‑C‑GC‑TGCGGGGGG‑TTTTTGCTTTTGGAGGGGCAGAAAGATTAATGAC‑TGTCCACGACGCTATACCCAAAAGAAAGCGGCtt                                                                                                       <  8:136293/151‑1 (MQ=17)
                                               gcTAGATTAATGAAGGCCCCCGAAATAACCCGTCTTTTCGGTAATTTTCACCTTTTTTAGTTAAAAACC‑C‑GG‑TTCGGGGGG‑TTTTTGCTTTTGTTGGGGATTAAATATTAATTAC‑TGTCCACGACGCTATACCCAAAAGAAAGCGGCTTAt                                                                                                     <  4:898500/150‑1 (MQ=2)
                                               ccTAGATGAATGACTGTCCACGACAGAAACCGGCTTATCGGTCAGTTTCACCTGATTTACCTAAAAACC‑C‑GC‑TTCGGCCGG‑ATTTTAATTTATGACGGGCAGAAACATGAAAGAA‑TATCAAACACACTATAAACAAAAGAAAGAGGCTTAt                                                                                                     >  8:807698/1‑151 (MQ=9)
                                                  aGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑GC‑TTCGGCGGG‑TTTTTGCTTTTGGAGGGGCAGAAAGATGAATGAC‑TGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCg                                                                                                   <  4:1440260/150‑1 (MQ=255)
                                                  aGATGAAGGACTGCCCACGACACAACCCGGCTTATCGGTCATTTTCACCTGATTTACGAAAAAACC‑C‑GC‑TTCGGCGGG‑TTTTTGCTTTTGGAGGGGCACAAACATGAATGAC‑TGCCCACGACGCTATACCCAAAAGAAAGCGGCTTATCg                                                                                                   <  1:1042315/150‑1 (MQ=17)
                                                    atgaatgaCGCTCCACTACAGAACCCGGCTTATGGGTCAGTTTCACCTGATTAACTTAAAAACC‑C‑GC‑TTCGGCGGG‑TTTTTGCTTTTGGAGGGGCAGAAAGATTAATGAC‑TGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTc                                                                                                <  8:1614435/151‑1 (MQ=14)
                                                     tgaatgaCTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑GC‑TTCGGCGGG‑TTTTTGCTTTTGGAGGGGCAGAAAGATGAATGAC‑TGTCCACGACGCTATACCCAAAAGAAAGCgggtgctcgcgc                                                                                                >  7:1345140/1‑140 (MQ=255)
                                                       attgtctatcctaaaCAGAACCCGGTTTATGGGCAATTTTCTCTTGATTTACTTAACACCG‑T‑TC‑TGCGGCGGG‑TTTTTGTGTTTGTTGGTGCCTAATTATGAATGAC‑TTTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAg                                                                                              <  6:796374/136‑1 (MQ=2)
                                                       aatgaCTCCCCACGCCAGAACCCGCCTTATCGGGCAGTTTCACCTGATTTACGTAAAACCC‑C‑GC‑TTCGGCGGG‑TTTTTGCTTTTGTAGGGGCACAAACATGAATGAC‑TGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAg                                                                                              <  4:190055/150‑1 (MQ=14)
                                                          ttctttcgtctaCAGAACCCTGCTTATCGGTATTTTTCACCTTATTTAGTTAAAACCG‑C‑GC‑TTCGGGGGG‑TTTTTGGTTTTGTTGGCGCAGAAAGATGAATGAC‑TTTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGttt                                                                                           <  6:1636507/139‑1 (MQ=14)
                                                          gaCTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑GC‑TTCGGCGGG‑TTTTTGCTTTTGGAGGGGCAGAAAGATGAATGAC‑TGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCgcggaggatc                                                                                          >  7:705209/1‑142 (MQ=255)
                                                           agtttcctgtactgAACCCGGTTTTTTGGTCTCTTTGACGTTATTTA‑GTTAAAACG‑G‑GTTTTCTGCTGG‑TTTTTGTTTTTGGAGTGTCTCAAATATTATTTAC‑TTTCCTCGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCa                                                                                         <  6:1332130/138‑1 (MQ=2)
                                                           aCTTTCCACGACAGAACCCGGCTTATCGGTAAGTTTCACCTGATTTACGTAAAAACC‑C‑GC‑TTCGGCGGG‑TTTTTGCTTTTGGAGGGGCAGAAAGATGATTGAC‑TGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTc                                                                                          <  4:957087/150‑1 (MQ=255)
                                                             tctCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑GC‑TTCGGCGGG‑TTTTTACTTTTGGAGGGGCAGaaa                                                                                                                                                    <  5:1334439/89‑1 (MQ=255)
                                                              ggccACGACAGAACCCGGATTATCGGTAATTTTCACGTGATTTACGAAAAACCC‑C‑GC‑TTCGGCGGT‑TTTTTGCTTTTGGAGGGGCCCAAACATGAATGAC‑TGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCt                                                                                      <  3:306697/149‑1 (MQ=14)
                                                              gcgctctaCAGAACCCGGCTTATGGGTAATTTTCACTTGATTTACTTAAAAACG‑C‑GC‑TTCGGCGGG‑TTTTTGTTTTTGTTGGGGCATTATTTTGATTGAC‑TTTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCAc                                                                                        <  2:393052/142‑1 (MQ=14)
                                                              gcccaccaCAGAACCGGGTTTATGGGTCAGTTTCACCTGTTTTACTTAAAACCC‑G‑GC‑TTGGGGGGT‑TTTTTGTTTTTGGTGGGGCTTAACTATGAATGAG‑TGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCt                                                                                      <  8:227271/149‑1 (MQ=2)
                                                               cgcACTACAGAACCCGGCTTATCGGTAATTTTCACGTGATTTAC‑TAAATACC‑CGGC‑TTCGGGGGG‑TTTTTGCTTTTGTTGGGGCAAAAATATGATTGAC‑TGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTg                                                                                     <  4:316698/149‑1 (MQ=14)
                                                                 caccacAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑G‑GC‑TTCGGCGGG‑TTTTTGCTTTTGTAGGGGCATAAAGATGATTGAC‑TGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGt                                                                                   <  4:1261040/151‑1 (MQ=255)
                                                                 cacaacacaaCCCGGATTATGGGAAATTTTCCCGTCATTTAGGAAAAAACC‑C‑TC‑TGCGGCGGG‑TTTTTGGTTTTGTTGGCGCTTAAATTTGAAGGAC‑TTGCCACGACGCTATACACAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGt                                                                                   <  5:1048220/151‑1 (MQ=9)
                                                                  aCGACAGAACCCGGGTTATCGGTAAGTTGCACGTGATTTACTAAAAAACC‑C‑GC‑TGCGGCGGG‑TTTTTGGTTTTGGCGGGGCATAAATATGAATGAC‑TGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGtt                                                                                  <  1:284927/151‑1 (MQ=14)
                                                                  aCGACAGAACCCGGCTTATCGGTAAGTTTCACCTGATTTACGTAAAAACC‑C‑GC‑TGCGGCGGG‑TTTTTGCTTTTGGAGGGGCACAAAGATGAATGAC‑TGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGtt                                                                                  <  7:1003703/151‑1 (MQ=255)
                                                                   cgccAGAACCCGGCTTAGCGGGCGGTTGCCCGTGATTTCCGTAAAACCC‑C‑GC‑TGCGGCGGG‑TTTTTGCTTTGGGCGGGGCAGAAAGATGATTGAC‑TGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGttt                                                                                 <  3:272988/148‑1 (MQ=14)
                                                                        caccccGGATTATCGGCATGTTGCACGTGATTTACTTAAAACCC‑C‑GC‑TGCGGCGGG‑TTTTTGCTTTTGCTGGGGCCCAAAGATGAATGAC‑TGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTa                                                                            <  3:782777/148‑1 (MQ=11)
                                                                         aaCCCGGCTTATCGGTCATTTGCCGGTCATTT‑CGGAATAACCGC‑TC‑TGCTGCGGG‑TTTTTGTTTTTGTTGGGGCAAAATTTTGATTGCC‑TCTCCAAGACGCTATACCCAAAAGAACGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTaa                                                                           <  4:1011125/151‑1 (MQ=9)
                                                                          aCCCGGCTTATCGGGCAGTTTCCCCTCATTTACGAAAAAACC‑C‑GC‑TTCGGCGGT‑TTTTTGCTTTTGGAGGGGCATAAAGATGAATGAC‑TGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTaaa                                                                          <  1:124565/151‑1 (MQ=14)
                                                                           cccGGCTGATCGGGCAGTTGCCCCTGATTTACGTAAAACCC‑C‑GC‑TGCGGCGGG‑TTTTTGCTTTTGGAGGGGCCGAAAGATGAATGAC‑TGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTaaaa                                                                         <  5:789697/151‑1 (MQ=11)
                                                                            ccGGCTAATCGGTCATTTTCGCTTGTTTTCCGTAAAAACG‑C‑GC‑TTCGGCGTG‑TTTTTGCTTTTGATGGGGCTTTATCATGAATGAG‑TTTCGACGACTCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTaaaaa                                                                        <  7:961934/151‑1 (MQ=11)
                                                                             cGGATTATCGGTAAGTTTCACCTGATTTAGCTAAAACCC‑C‑GC‑TTCGGCGGG‑TTTTTGGTTTTGGGGGGGCAGAAAGATGAATGAC‑TCTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAAc                                                                       <  1:1557760/151‑1 (MQ=11)
                                                                               gCTTATGGGTAAGTTTCCCCTTATTTCCGAAAAACCG‑C‑GC‑TTCGGCGGG‑TTTTTGTTTTTGTCGGGGCACAAACTTGAATGAC‑TGCCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAAccc                                                                     <  7:35300/151‑1 (MQ=11)
                                                                                  tATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑GC‑TTCGGCGGG‑TTTTTGCTTTTGGAGGGGCTGAAACATGAATGAC‑TGGCCCGGACCGTATCCGCAATAGAAAGCGGCGTATCGGTCAGTTTAACCTGCTGTACGTAATAACCCggg                                                                  >  1:1338043/1‑149 (MQ=11)
                                                                                   atgggtaattttCCCCTGATTTACTTAAAACCG‑G‑CC‑TGCGGCGGG‑TTTTTGTTTTTGCTGGCGCTCAAATATGAATGAC‑TCTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCtt                                                                 <  4:805811/142‑1 (MQ=11)
                                                                                     cGGTCAGTTTCACCTGATTTACGTAAAAACC‑G‑GC‑TTCGGCGGG‑TTTTTGGTTTTGGAGGGGCAGAAAGAGGAATGAC‑TGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTc                                                                <  1:16530/150‑1 (MQ=255)
                                                                                     cGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑GC‑TTCGGCGGG‑TTTTTGCTTTTGGAGGGGCAGAAAGATGAATGAC‑TGTCCACGACGCTATACCCAAAAGCAAGCGGCTTATCGGTCAGTTTCACCTGGTGTACGTAATAACCCggtgcg                                                               >  1:431906/1‑146 (MQ=255)
                                                                                     cGGTAATTTTGACCTCATTTAGTTAAAAACG‑C‑TC‑TGAGGCGGG‑TTTTTGCTTTTGAAGGCGCATAAACATGAATGAC‑TTTCCACGACTCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTc                                                                <  2:1193037/150‑1 (MQ=11)
                                                                                      ggTCAGTTTCCCTTGATTTTGGTAAAACCC‑A‑GG‑TT‑GTCGGGTTTTTTGTTTTTTTTGGCTCATAAATTTGATTGAC‑TTTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTcgg                                                              <  2:799160/151‑1 (MQ=2)
                                                                                      ggTCAGTTTCACCTGATTTACGTAAAAACC‑C‑GC‑TTCGGCGGG‑TTTTTGCTTTTGGAGGGGCAGAAAGATGAATGAC‑TGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGGTGTACGTAATAAccggtggggg                                                              >  1:458899/1‑143 (MQ=11)
                                                                                       ggaacttgcACGTGATTAAGTTAAACCCC‑G‑GC‑TGCGGGGGG‑TTTTTGTTTTTGGTGGGGCTCAACAATGATGGCC‑TCGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTcggc                                                             <  7:1159731/143‑1 (MQ=2)
                                                                                       gTCACTTTCACGTGATTTCCTTAAACCCG‑C‑GC‑TTCGGCGGG‑TTTTTGTTTTTGCTGGGGCATAAAGATGATTGAC‑TTGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTcggc                                                             <  8:1087892/151‑1 (MQ=11)
                                                                                                cccTCATTAACGTAAAAACC‑C‑CC‑TGCGGCGGG‑TTTTTGCTGTTGCCCGGGCCCAACCATGAATGAC‑TCGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTg                                                    <  1:1005145/150‑1 (MQ=11)
                                                                                                    caTTTACGTAAACCCC‑C‑CC‑TGCGGCGGG‑TTTTTGCTTTTGCCGGGGCCCAACCATGACTGCC‑TGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCttt                                                <  3:1110017/150‑1 (MQ=11)
                                                                                                     aTTTACGAAAACCCC‑C‑GC‑TGCGGCGGG‑TTTTTGCTTTTGCCGGCGCCCAAAGATGAATGAC‑TGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCtttt                                               <  5:237444/151‑1 (MQ=11)
                                                                                                        tACGTAAAAACC‑C‑GC‑TTCGGCGGG‑TTTTTGCTTTTGGAGGGGCAGAAAGATGAATGAC‑TGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGGTGTACGTAATAACCCGGTGCGGCGGGTTTGTGCtttttgc                                            >  3:219745/1‑148 (MQ=11)
                                                                                                          cGTAAAAACC‑C‑GC‑TTCGGCGGG‑TTTTTGCTTTTGCCGGGGCCCAACCATGCAGGAC‑TCTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAgg                                          <  3:1414598/151‑1 (MQ=11)
                                                                                                            tAAAAACG‑T‑GC‑TTCGGCGGG‑TTTTTGTTTTTGTTGTTGCTTAATTTTTATTGAG‑TTTCCACTACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAggg                                         <  2:642123/150‑1 (MQ=11)
                                                                                                            aaaaacCC‑C‑CC‑TGCGGCGGG‑TTTTTGCTTTTGCCGGCGCACAACGATGAAGGCC‑TGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAgggg                                        <  7:1136085/150‑1 (MQ=11)
                                                                                                            aaaaaaCC‑G‑TC‑TTCGGCGGG‑TTTTTGGTTTTGGTGGTGCTTAAATATGAATGAC‑TGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAggg                                         <  8:144418/149‑1 (MQ=11)
                                                                                                              aaacCC‑C‑GC‑TTCGGCGGG‑TTTTTGCTTTTGCCCCGGCCGCACCATGCAGGCC‑TCGCCACCACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCa                                      <  5:843176/151‑1 (MQ=11)
                                                                                                              aaaaCG‑C‑GC‑TGCGGCGGG‑TTTTTGGTTTTGGCGGGGCATAACGATGATTGAC‑TGTCCACGACGCTATACCCAACAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCa                                      <  2:1347565/151‑1 (MQ=11)
                                                                                                                aacacccgcctgcggcggG‑TTTTTGGTTTTGCCGGGGCCCAACCATGCATGCC‑TCGCCACGACGCTATACCCAACAGAAACCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAggggt                                       <  7:1526793/139‑2 (MQ=11)
                                                                                                                 cccccggC‑TGCGGCGGG‑TTTTTGGTTTTGCCGCGGCCGAATAATGACTGCC‑TGTCCACGACGCTATACCCAACAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAg                                     <  3:80976/145‑1 (MQ=11)
                                                                                                                   c‑c‑GC‑TTACGCGGG‑TTTTTGTTTTTGGCGGCGCGCCACCATGAAGGAC‑TCTCGACTACGCTCTACCCAAAAGAACCCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAg                                 <  1:554220/151‑1 (MQ=11)
                                                                                                                       gC‑TTCGGCGGG‑TTTTTGCTTTTGGAGGGGCAGAAAGATGAATGAC‑TGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGGTTTACGTAAAAACCCGCTGCGGCGGGTTTGAGATTTTgatcggacgggaag                                 >  3:1526207/1‑136 (MQ=11)
                                                                                                                         gtgccgggtg‑tttttgtttttGGGGGGGCCAAATATTGATGGAC‑TGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGatg                              <  7:375785/142‑1 (MQ=11)
                                                                                                                          ttCGGCGGG‑TTTTTGCTTTTGGAGGGGCAGACATATGAATGCC‑TGACGTCTCACCTAGACACAAATGAACCCCGCTGCTCGGGCCGGTTCACCTGGTTTACCTAAAAACCCGGTTCGGCGGGGTGTTGGTTTGGGCGGGGcggcggggggg                             >  3:1327227/1‑141 (MQ=2)
                                                                                                                           gcgggtgg‑TTTTTTTTTTTGGTGGTGCTTAATTTTGATTGAC‑TTTCCTCGACTCTATACCCTAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGatga                             <  8:620303/144‑1 (MQ=11)
                                                                                                                            cggcggG‑TTTTTGCTTTTGGCGGGGCCTAACCATGAATGCC‑TTGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGatgaat                           <  5:888441/151‑1 (MQ=11)
                                                                                                                             gccggG‑TTTTTGGTTTTGCGCGTGCATACCAATGATTGCC‑TGGCCACGACGCTATACACAAAAGAACGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTGGGAGGGGCAGAAAGatgaatg                          <  5:1025394/149‑1 (MQ=11)
                                                                                                                                 gg‑TTTTTGCTTTTGGAGGGGCAAAAACATGATGGAC‑TGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTg                      <  1:1456641/151‑1 (MQ=255)
                                                                                                                                                       aGAAAGATGAATGAC‑TGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGGTTTTGGAGGGGCAGAAAGATGAGTTACTGACCACGACACAATACCCtaa  >  3:679575/1‑150 (MQ=11)
                                                                                                                                                       aGAAAGATGAATGAC‑TGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAACCCGATACGGCGGGTTTAAGATTTTTGAGTGCCAGAAATAATAAAGACTGACCACTAAACtaaaaaaaaa  >  4:554866/1‑142 (MQ=2)
                                                                                                                                                        |                                                                                                                                                     
CCGTACTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑GC‑TTCGGCGGG‑TTTTTGCTTTTGGAGGGGCAGAAAGATGAATGAC‑TGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAA  >  DH1/613449‑613745

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑