BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	DH1	613,613	0	T	G	27.1%	184.0 / 34.5	201	intergenic (+474/+549)	garK/yhaC	glycerate 2‑kinase/pentapeptide repeats‑related protein
Reads supporting (aligned to +/- strand): ref base T (44/96); new base G (3/49); total (49/152)
Fisher's exact test for biased strand distribution p-value = 1.17e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 208 total aligned reads displayed.

GGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCG‑GTCAGTTTCACCTGATTTACGTAAAAACC‑C‑GCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGG  >  DH1/613463‑613754

 gTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAAGACGGATGATAG‑GACAGGATCACGTGAGTAAAGTAAAAACA‑A‑GCTTCGTCGGCTTGTTTCTTGTGGAGGGGAATAAAGATGAATGACTGTc                                                                                                                                              >  7:151626/1‑151 (MQ=255)

  tAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCG‑GTCAGTTTCACCTGATTTAAGTAAAAACC‑C‑GCTTAAGCCGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGta                                                                                                                                              >  2:902726/1‑149 (MQ=25)

   aGGCTGCTTGAGCCTGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCG‑GTCAGTTTCACCTGATTTACGTAAAAACC‑C‑GCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTcc                                                                                                                                             <  2:1537358/150‑1 (MQ=255)

   aGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAGTGACTGTCCACGACAGAACCCGGCTTATCG‑GTCAGTTTCACCTGATTTACGTAAAAACC‑C‑GCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTcc                                                                                                                                             <  2:1000073/150‑1 (MQ=255)

     gctgctTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCG‑GTCAGTTTCACCTGATTTACGTAAAAACC‑C‑GCTTCGGCGGGTTTTTGCTTTTGGAGTGGCAGAAAGATGAATGCCTGTCCacg                                                                                                                                          >  7:360005/1‑151 (MQ=255)

     gctgctTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCG‑GTCAGTTTCACCTGATTTACGTAAAAACC‑C‑GCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCacg                                                                                                                                          <  5:579993/151‑1 (MQ=255)

      ctgctTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCG‑GTCAGTTTCACCTGATTTACGTAAAAACC‑C‑GCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGtagatcc                                                                                                                                         >  3:282875/1‑145 (MQ=255)

       ggctTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCG‑GTCAGTTTCACCTGATTTACGTAAAAACC‑C‑GCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCacgac                                                                                                                                        <  8:234797/150‑1 (MQ=255)

        gctTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCTGCTTATCG‑GTCAGTTTCACCTGATTTACGTAAAAACC‑C‑GCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCacgac                                                                                                                                        <  2:1505374/150‑1 (MQ=255)

        gctTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCG‑GTCAGTTTCACCTGATTTACGTAAAAACC‑C‑GCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCacgacg                                                                                                                                       <  2:680324/151‑1 (MQ=255)

        gctTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCG‑GTCAGTTTCACCTGATTTACGTAAAAACC‑C‑GCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCacgac                                                                                                                                        <  5:795795/150‑1 (MQ=255)

        gctTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCG‑GTCAGTTTCACCTGATTTACGTAAAAACC‑C‑GCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCacga                                                                                                                                         <  7:586457/149‑1 (MQ=255)

        gctTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCG‑GTCAGTTTAACCTGATTTAAGTAAAAACC‑C‑TCTTCTACGGGTATTTAGTTTTGGATTGTCTGAAAGAAGAATGACTGTCAacgaa                                                                                                                                        >  8:439881/1‑149 (MQ=21)

           tGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCG‑GTCAGTTTCACCTGATTTACGTAAAAAAC‑C‑GCTTCCGCGGGTTTTTGGGTTTGGAGGGGATGAAAGATGAATGAATGTCCACGACGCt                                                                                                                                     >  7:755841/1‑150 (MQ=25)

             cgCCAGTGATCTATTTCTGGCCTAGATTAATGACTCTCCACTACAGAACCCGGATTATGT‑GTAAGTTTCACCTGATTTACTTAAAAACC‑C‑GCTTCGGCGGTTTTTTGCTTTTGTAGGGGCAGAAATATGAATGACTGTCCACGACGCtata                                                                                                                                  <  6:1057193/150‑1 (MQ=255)

              gCCAGTGAGCGATTGCTGGCCTAGATTAATGACTGTCCACGACAGAACCCGGCTTATGG‑GTCAGTTTCACCTGATTTACGTAAAAACC‑C‑GCTTCGGCGGTTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGGCCACGACGCTATAc                                                                                                                                 <  3:317489/151‑1 (MQ=255)

                cAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACACGGCTTATCG‑GTCACTTTCACCTGATTTAAGTAAAAAAC‑C‑TATTCGGAGGGGTTTTGCTTTTGGAGGTGCAGAAAGATGAATGAGTGTCCACGACGGTAGAccc                                                                                                                               >  7:1368954/1‑151 (MQ=21)

                       cGATTGCTGGCCTAGATGAATGACTGTCCACGACACAAACCGCCTAATAG‑GTCAGATTCAACAGATTTACCTAAAAAGC‑A‑ACTTAAACGGGTTTAAGCTATTGGAAGAGCACAAAGATGAATGAATCTAAAAAACAATATACCCAaaaga                                                                                                                         >  4:742259/1‑150 (MQ=255)

                       cGATTGCTGGCCTAGATGAATGACTGTCCACGACACAAAACCCATAATCA‑ATCACTTTCACCTGATTTACGTAAAAACA‑A‑ACTTAGTCCGGATATTTCTTTAGGAGGGGCAGAAAGATGAATGAATGTAAAAGACACTATACCCAaaaga                                                                                                                         >  2:457471/1‑150 (MQ=12)

                         aTTGCGGCCCTATATTAATGACTGCCCCCGACATAACCCGTGTTAGCG‑GTCATTTCCACCTTATTTACTTAAAAACC‑G‑GGTTCGGGGGTTTTTTGATTTTGGCGGGGCATAAACATCAATCACTGGCCACGACGCTATACCCAaaagaaag                                                                                                                      <  8:773755/151‑1 (MQ=255)

                          ttGCTGGCCTAGATGAATGACTGTCCACCACAGAACCCGGCTTAGCG‑GTCAGTTTCACCTGATTTACGTAAAAACC‑C‑GCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAaaagaaag                                                                                                                      <  6:1382233/150‑1 (MQ=255)

                           tGCTGGCCTAGATGAATGACTGGCCACGACAGAACCCGGCTTATCG‑TTCAGTTTCACCTGATTTACGTAAAAACC‑C‑GCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGc                                                                                                                     <  4:903852/150‑1 (MQ=255)

                           tGCTGGCCTAGATGAATGACTGGCCACGACAGAACCCGGCTTATCG‑GTCAGTTTCACCTGATTTACTTAAAAACC‑G‑GCTTCGGCGGGTTTTTGCTTTTGGCGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGc                                                                                                                     <  2:1053491/150‑1 (MQ=255)

                           ggcgggCCAAGATGACTGACTGGCCACGACAGAACCCGGCTTATCG‑GTCAGTTTCACCTGATTTACGTAAAAACC‑C‑GCTTCGGCGGGTTTTTGCTTTTGGCGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCg                                                                                                                    <  4:883759/147‑1 (MQ=255)

                            gcgggCCTAGTGTAATGACTGTCCCCGCCAGACCCCGGCTTCGCG‑GTCAGTTTCACCTGATTTACGTAAAACCC‑C‑GCTTCGGCGGGTTTTTGCTTTTGTAGGGGCACAAAGATGAATGACTGGCCACGACGCTATACCCAAAAGAAAGCg                                                                                                                    <  4:361099/147‑1 (MQ=14)

                              tGGCCTAGATGAATGACTGTCCACGACACAAAACGGAATATCG‑TACAGTTTCACCAGATTTACCAAAAAACA‑A‑TATTAATCGGGTTTTTCCTTTTGTATTTACATAAAGATGAATGACTGTCCGCGTGACTGTGCGCAACAGAGAGCGGCt                                                                                                                 >  2:125869/1‑151 (MQ=255)

                               ggCCTAGATTATTCCCTGTCCACTACAGAACCCGGCTTATCG‑GTCAGTTTCACCTTATTTACTTAAAAACC‑C‑GCTGGGGGGGTTTTTTGGTTTTGGTGGGGCAGAAAGATTAATGACTGGCCACGACGCTATACCCAAAAGAAAGCGGCtt                                                                                                                <  5:460580/151‑1 (MQ=14)

                               ggCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCG‑GTCAGTTTCACCTGATTTACTTAAAACCC‑C‑GCTTCGGCGGGTTTTTGCTTTTGGCGGGGCAGAAACATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCtt                                                                                                                <  4:1262594/151‑1 (MQ=255)

                               ggCCTAGATGAATGACTGCCCACCACAGAACCCGGCTTAGCG‑GTCTGTTTCACCTTATTTACGTAAAACCC‑C‑GCTGCGGGGGGTTTTTGCTTTTGGAGGGGCAGAAAGATTAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCtt                                                                                                                <  8:136293/151‑1 (MQ=17)

                                gCCTAGATCAATGACTGTCCACGACAGAACCCGGCTTATCG‑GTCATTTTCACCTGATTTACTTAAAAACC‑C‑GCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATTAATGACTTTCCACGACGCTATACCCAAAAGAAAGCGGCTTa                                                                                                               <  6:1138847/151‑1 (MQ=255)

                                 cgtatttaactgccTGCCCACGACATAACGCGGGTTAGCG‑GTCTGTTTAAGCTTATTTACTTAAAAACC‑C‑GGTTCGGGGGGTTTTTGTTTTTGGTGGGGCAAAATGTTGATTCAGTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTAt                                                                                                              <  8:182460/138‑1 (MQ=9)

                                 ccgcccccacacccTGTACACTAAAGAACCCGCCTTAGCG‑GTCAGTTTCACCTGGTTTTCTTAAAACCC‑C‑CCTGGGGGGGTTTTTTGCTTTTGTTGGGGCAGAAAGATGAATGACTGACCACGACACTATACCCAAAAGAAAGCGGCTTAt                                                                                                              <  3:742029/138‑1 (MQ=0)

                                    aGATGAATGACTGTCCACGACAGAACCCGGCTTATCG‑GTCAGTTTCACCTGATTTACGTAAAAACC‑C‑GCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCg                                                                                                            <  4:1440260/150‑1 (MQ=255)

                                    aGATGAAGGACTGCCCACGACACAACCCGGCTTATCG‑GTCATTTTCACCTGATTTACGAAAAAACC‑C‑GCTTCGGCGGGTTTTTGCTTTTGGAGGGGCACAAACATGAATGACTGCCCACGACGCTATACCCAAAAGAAAGCGGCTTATCg                                                                                                            <  1:1042315/150‑1 (MQ=17)

                                     gATGAATGACTGTCCACGACAGAACCCGGCTTATCG‑GTCAGTTTCACCTGATTAACGTAAAAACC‑C‑GCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGt                                                                                                          <  1:310164/151‑1 (MQ=255)

                                     gATGAATGACGGGCCACGCCAGAACCGGGCTTATCG‑GTCAGTTTCACCTGATTTACTTAAAAACC‑C‑GCTGCGGCGGGTTTTTGCTTTTGGAGGGGCACAAAGATCAATGACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGt                                                                                                          <  7:1287608/151‑1 (MQ=14)

                                      atgaatgaCTGTCCACGACAGAACCCGGCTTATCG‑GTCAGTTTCACCTGATTTACGTAAAAACC‑C‑GCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACCCTATACCCAAAAGAAAGCGGCTTAtgctgg                                                                                                         >  7:1553825/1‑146 (MQ=255)

                                       tgaatgaCTGTCCACGACAGAACCCGGCTTATCG‑GTCAGTTTCACCTGATTTACGTAAAAACC‑C‑GCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCgggtgctcgcgc                                                                                                         >  7:1345140/1‑140 (MQ=255)

                                       tgaatgaCTGTCCACGACAGAACCCGGCTTATCG‑GACAGGTTCACGTGATTTACGTAAAAACC‑A‑GCTTCGGCGGGTTTTTGCTTTTGGAGGGGAAGAAAGATGAATGACTGTCCACGGCGCTATACCGAACGGAACGGGGCTGCTCGGTcc                                                                                                        >  1:1264858/1‑150 (MQ=17)

                                       cgcaggactccccccGACAGAACGGGGCTTATGG‑TTCTGTTTGCCGTTGTTTCCTTAATAACC‑C‑GGTTCGGCTGTTTTTTGCTTTTGGGGGGGCACTTCCATTATTGACTGGCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCa                                                                                                        <  1:1531763/137‑1 (MQ=2)

                                        gaatgaCTGTCCACGACAGAACCCGGCTTTTCG‑GTCATTTTCACCTGATTTACGTAAAAACC‑C‑GCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCa                                                                                                        <  1:368080/150‑1 (MQ=255)

                                         aatgaCTCCCCACGCCAGAACCCGCCTTATCG‑GGCAGTTTCACCTGATTTACGTAAAACCC‑C‑GCTTCGGCGGGTTTTTGCTTTTGTAGGGGCACAAACATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAg                                                                                                       <  4:190055/150‑1 (MQ=14)

                                          atgaCTGTCCACGACAGAACCCGGCTTATCG‑GTCAGTTTCACGTGATTTACGTAAAAACC‑C‑GCTTCGGCGGGTTTTTGCTTTTGGTGGGGCACAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGtt                                                                                                     <  2:449140/151‑1 (MQ=255)

                                            ttctttcgtctaCAGAACCCTGCTTATCG‑GTATTTTTCACCTTATTTAGTTAAAACCG‑C‑GCTTCGGGGGGTTTTTGGTTTTGTTGGCGCAGAAAGATGAATGACTTTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGttt                                                                                                    <  6:1636507/139‑1 (MQ=14)

                                            ggcTGTCCAGGTCAGAACCCTGCTTTTCGTGTC‑TTTTCTCTTCATTTACTTATATAGG‑G‑GGTTCTGCGGGTTTTTGTTTTTGGTGGTGCTGAAATTTTAATTACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGttt                                                                                                    <  6:875137/148‑1 (MQ=2)

                                            gaCTGTCCACGACAGAACCCGGCTTATCG‑GTCAGTTTCACCTGATTTACGTAAAAACC‑C‑GCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCgcggaggatc                                                                                                   >  7:705209/1‑142 (MQ=255)

                                             aCTTTCCACGACAGAACCCGGCTTATCG‑GTAAGTTTCACCTGATTTACGTAAAAACC‑C‑GCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGATTGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTc                                                                                                   <  4:957087/150‑1 (MQ=255)

                                                gcccaccaCAGAACCGGGTTTATGG‑GTCAGTTTCACCTGTTTTACTTAAAACCC‑G‑GCTTGGGGGGTTTTTTGTTTTTGGTGGGGCTTAACTATGAATGAGTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCt                                                                                               <  8:227271/149‑1 (MQ=2)

                                                 cgcACTACAGAACCCGGCTTATCG‑GTAATTTTCACGTGATTTAC‑TAAATACC‑CGGCTTCGGGGGGTTTTTGCTTTTGTTGGGGCAAAAATATGATTGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTg                                                                                              <  4:316698/149‑1 (MQ=14)

                                                   cacaacacaaCCCGGATTATGG‑GAAATTTTCCCGTCATTTAGGAAAAAACC‑C‑TCTGCGGCGGGTTTTTGGTTTTGTTGGCGCTTAAATTTGAAGGACTTGCCACGACGCTATACACAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGt                                                                                            <  5:1048220/151‑1 (MQ=9)

                                                    aCGACAGAACCCGGGTTATCG‑GTAAGTTGCACGTGATTTACTAAAAAACC‑C‑GCTGCGGCGGGTTTTTGGTTTTGGCGGGGCATAAATATGAATGACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGtt                                                                                           <  1:284927/151‑1 (MQ=14)

                                                    aCGACAGAACCCGGCTTATCG‑GTAAGTTTCACCTGATTTACGTAAAAACC‑C‑GCTGCGGCGGGTTTTTGCTTTTGGAGGGGCACAAAGATGAATGACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGtt                                                                                           <  7:1003703/151‑1 (MQ=255)

                                                     cGACAGAACCCGGCTTATCG‑GTCAGTTTAGCGGGCTTTACGTAAAAACA‑C‑GCGTCGGCGCGGGTTTTCGTTTGGAGGGGCAGCAAGAGGACTTCCTGTCCACGACGCTAGACCCCGAAGACGGCGGCGGATCGGGCAGtgtggcggggggg                                                                                          >  1:865133/1‑139 (MQ=255)

                                                       aCAGAACCCGGCTTATCG‑GGAAGTTTCCGCTCATTTAGTTACAACCC‑C‑GCTGCGGCGGGTTTTTGGTTTTGCTGGGGCCTAAACTTGATTGACTTGCCACTACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTAc                                                                                        <  8:1088634/151‑1 (MQ=9)

                                                          caccccGGATTATCG‑GCATGTTGCACGTGATTTACTTAAAACCC‑C‑GCTGCGGCGGGTTTTTGCTTTTGCTGGGGCCCAAAGATGAATGACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTa                                                                                     <  3:782777/148‑1 (MQ=11)

                                                           aaCCCGGCTTATCG‑GTCAGTTTCACCTGATTTACGTAAAAACC‑C‑GCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCgg                                                                                                                   >  4:1142080/1‑120 (MQ=18)

                                                            aCCCGGCTTATGT‑GGAAGTTTCCGATGATTTACGTAAAAACC‑C‑GCTTCGGCGTGTTTTTGTTTTTGGAGGGGCCGAAATATGACTGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTaaa                                                                                   <  3:24865/151‑1 (MQ=14)

                                                            aCCCGGCTTATCG‑GGCAGTTTCCCCTCATTTACGAAAAAACC‑C‑GCTTCGGCGGTTTTTTGCTTTTGGAGGGGCATAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTaaa                                                                                   <  1:124565/151‑1 (MQ=14)

                                                              ccGGCTAATCG‑GTCATTTTCGCTTGTTTTCCGTAAAAACG‑C‑GCTTCGGCGTGTTTTTGCTTTTGATGGGGCTTTATCATGAATGAGTTTCGACGACTCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTaaaaa                                                                                 <  7:961934/151‑1 (MQ=11)

                                                                 gattATCG‑GGATGTTTCCCCTCATTTTCTTAATACCC‑C‑GCTTCGGCTGGTTTTTGCTTTTGCCGGCGCATAACTATGCAGGACTTGACACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTTCGTAAAAAccc                                                                              <  2:497050/149‑1 (MQ=2)

                                                                 gCTTATCG‑GGCTTTTTCACCTGATTAAGTTAAAACCC‑G‑GCTTCGGCGGGTTTTTGGTTTTGCCGGGGCCCAATGTTGAATGAGTGCCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAAccc                                                                              <  1:468717/151‑1 (MQ=11)

                                                                   ttATCG‑GTCAGTTTCACCTGATTTACGTAAAAACC‑C‑GCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTCTCGACGACCCGATGGCCAAATCAAAGAGGCTTATGGCGCAGTTTCGCCTGATTTACGTAAAAACCCgg                                                                            >  5:771110/1‑150 (MQ=11)

                                                                     aTCG‑GTCATTTGCACCTTATTTCCGTAAAACCC‑C‑TCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAACGATTATTGACTTTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCt                                                                           <  1:1389154/150‑1 (MQ=11)

                                                                       gg‑gTCATTTTCACCTGATTACCTTAAAACCC‑C‑GCTGCGGCGGGTTTTTGGTTTTGGCGGGGCAGAAAGATGAAGGACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTcg                                                                        <  1:93487/150‑1 (MQ=11)

                                                                       cG‑GTCAGTTTCACCTGATTTACGTAAAAACC‑G‑GCTTCGGCGGGTTTTTGGTTTTGGAGGGGCAGAAAGAGGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTc                                                                         <  1:16530/150‑1 (MQ=255)

                                                                        gtgtaaGTTGCCCCTCATTT‑CGTTAAAACCGG‑GCTTCGGCGGTTTTTTGGTTTTGCCGGGGTTCAACCATGAATGACTGGCCACGACGCTATACACAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTcg                                                                        <  5:149974/146‑1 (MQ=2)

                                                                        ggggcAGTTTCCCCTGATTAACTTAAAAACC‑C‑GCTTCGGCGGGTTTTTGCTTTTGGAGGGGCATAAACTTGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTcg                                                                        <  7:1068733/147‑1 (MQ=11)

                                                                        cgggaaGTTTCACCTGATTTAGGTAAAACCC‑C‑GCTTCGGCGGGTTTTTGCTTTTGGAGGGGCACCAAGATGAATGCCTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTc                                                                         <  1:434151/145‑1 (MQ=11)

                                                                         gggaaGTTGCAGCTGATTAAGGTAACACCC‑C‑GCTGCGGCGGGTTTTTGCTTTTGGCGGGGCCCAACGAAGATTGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTcgg                                                                       <  3:313577/147‑1 (MQ=11)

                                                                          gtaattttCACGTGATTTACGTAAAACCC‑C‑GCTTCGGGGGGTTTTTGGTTTTGGAGGGGCACAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTcggc                                                                      <  3:540654/146‑1 (MQ=11)

                                                                          ggaacttgcACGTGATTAAGTTAAACCCC‑G‑GCTGCGGGGGGTTTTTGTTTTTGGTGGGGCTCAACAATGATGGCCTCGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTcggc                                                                      <  7:1159731/143‑1 (MQ=2)

                                                                                ttCACCTGATTTACGTAAAAACC‑C‑GCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGGTTTACGTAATAACCCGTTCCGGCGGGttt                                                                >  3:838378/1‑151 (MQ=255)

                                                                                 gccccTCATTTACGCAAACCCC‑C‑GCTGCGGCGGGTTTTTGCTTTGGGCGGGGCCCAAAGATGAATGACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGtttt                                                               <  5:1135761/148‑1 (MQ=11)

                                                                                   cccTCATTAACGTAAAAACC‑C‑CCTGCGGCGGGTTTTTGCTGTTGCCCGGGCCCAACCATGAATGACTCGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTg                                                             <  1:1005145/150‑1 (MQ=11)

                                                                                    ccTGATTTACGTAAAAACC‑C‑GCATCGGAAGGTTTTAGATTTTGGAAAGACAGAAAGATGAATGAATGTACACGACGCTATACCAAAAATAAAAAAACTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCCCATcggaagcgagtggg                                                            >  8:1255442/1‑140 (MQ=2)

                                                                                     cTGATTTACGTAAAAACC‑C‑GCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGGTGTACGTAATAACCCGGTGCGGCGGGTTTGTGCt                                                           >  1:1587179/1‑151 (MQ=11)

                                                                                       gATTTACGTAAACACC‑C‑GCTGCGGCGGGTTTTTGCTTTTGGAGGGGCCCAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCt                                                           <  7:669826/149‑1 (MQ=255)

                                                                                       caTTTACGTAAACCCC‑C‑CCTGCGGCGGGTTTTTGCTTTTGCCGGGGCCCAACCATGACTGCCTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCttt                                                         <  3:1110017/150‑1 (MQ=11)

                                                                                        aTTTACGAAAACCCC‑C‑GCTGCGGCGGGTTTTTGCTTTTGCCGGCGCCCAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCtttt                                                        <  5:237444/151‑1 (MQ=11)

                                                                                        aTTTACGAAAAAACG‑C‑GCTTCGGCGGGTTTTTGGTTTTGCTGGGGCACAATCATGAAGCCCTGTACACGACGCTATACCCTAAAGAAAGCGGCGTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCttt                                                         <  2:1427942/150‑1 (MQ=11)

                                                                                           aacttAAAAACC‑C‑GCTTCGCCGGGTTTTTGCTTTTGCTGGGGCCTAAAGTTTAATGACTGCCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGGCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGa                                                     <  8:831576/147‑1 (MQ=11)

                                                                                            aCGTAAAAACC‑C‑GCTTCGGCGGGTTTTTGCTTTTGCAGGGGCCGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAg                                                    <  7:305942/151‑1 (MQ=25)

                                                                                             cGTAAAAACC‑C‑GCTTCGGCGGGTTTTTGCTTTTGCCGGGGCCCAACCATGCAGGACTCTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAgg                                                   <  3:1414598/151‑1 (MQ=11)

                                                                                               aaaaacCC‑C‑CCTGCGGCGGGTTTTTGCTTTTGCCGGCGCACAACGATGAAGGCCTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAgggg                                                 <  7:1136085/150‑1 (MQ=11)

                                                                                               aaaaaaCC‑G‑TCTTCGGCGGGTTTTTGGTTTTGGTGGTGCTTAAATATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAggg                                                  <  8:144418/149‑1 (MQ=11)

                                                                                                aaaaaCC‑C‑GCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAAAATAAGAACAGACTCCTCCACTATACCAAAAAGACAGGATGTTTACCGAGAGTTTCACCACGTTTACGTAAAAAAAAGCTTCGGGGGGGGTTTGGTTTTGggggggg                                                >  6:748494/1‑150 (MQ=2)

                                                                                                 aaacCC‑C‑GCTTCGGCGGGTTTTTGCTTTTGCCCCGGCCGCACCATGCAGGCCTCGCCACCACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCa                                               <  5:843176/151‑1 (MQ=11)

                                                                                                    cccccggCTGCGGCGGGTTTTTGGTTTTGCCGCGGCCGAATAATGACTGCCTGTCCACGACGCTATACCCAACAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAg                                              <  3:80976/145‑1 (MQ=11)

                                                                                                     accgtgCTTCGGCGTGTTTTTGGTTTTGCCGGTGCCTCCACTTGCTTGACTTTCCACGAGGCTATACCCAACAGAACGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGa                                             <  8:245903/146‑1 (MQ=11)

                                                                                                     accctcctgatgcggGTTTTTGCGTTTGCTGGGGCCCTACCATGAATGACTCGACACTACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGa                                             <  2:1155317/140‑1 (MQ=11)

                                                                                                          gCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGGTTTACGTAAAAACCCGCTGCGGCGGGTTTGAGATTTTgatcggacgggaag                                          >  3:1526207/1‑136 (MQ=11)

                                                                                                           gtgccgggtgtttttgtttttGGGGGGGCCAAATATTGATGGACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGatg                                       <  7:375785/142‑1 (MQ=11)

                                                                                                            ttCGGCGGGTTTTTGCTTTTGGAGGGGCAGACATATGAATGCCTGACGTCTCACCTAGACACAAATGAACCCCGCTGCTCGGGCCGGTTCACCTGGTTTACCTAAAAACCCGGTTCGGCGGGGTGTTGGTTTGGGCGGGGcggcggggggg                                      >  3:1327227/1‑141 (MQ=2)

                                                                                                             gcgggtggTTTTTTTTTTTGGTGGTGCTTAATTTTGATTGACTTTCCTCGACTCTATACCCTAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGatga                                      <  8:620303/144‑1 (MQ=11)

                                                                                                              cggcggGTTTTTGCTTTTGGCGGGGCCTAACCATGAATGCCTTGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGatgaat                                    <  5:888441/151‑1 (MQ=11)

                                                                                                               gccggGTTTTTGGTTTTGCGCGTGCATACCAATGATTGCCTGGCCACGACGCTATACACAAAAGAACGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTGGGAGGGGCAGAAAGatgaatg                                   <  5:1025394/149‑1 (MQ=11)

                                                                                                               cgcggGTTTTTGCTTTTGCAGGCGCAGAACAATGAATGACTTGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGatgaatg                                   <  3:170621/150‑1 (MQ=11)

                                                                                                                 cggGTTTTTGCTTTTGGTGGGGCAGAACTATGAATGACTCGCCAAGACGCTATACCCAAAAGAATGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGAc                                 <  3:615223/151‑1 (MQ=11)

                                                                                                                    gTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACGGTCCACGACGCTAAAACAAAAACAAAAAGTCTTATAGTACAGTTTCACCTGATATAATAAAAAAAAAGCTTCGGCGGGTTTTTGCGTTTGGAGGGGCTGaagctgggatgagggg                              >  6:863578/1‑137 (MQ=2)

                                                                                                                                        aGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAACCCGATACGGCGGGTTTAAGATTTTTGAGTGCCAGAAATAATAAAGACTGACCACTAAACtaaaaaaaaa           >  4:554866/1‑142 (MQ=2)

GGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCG‑GTCAGTTTCACCTGATTTACGTAAAAACC‑C‑GCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGG  >  DH1/613463‑613754

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`