BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	DH1	2,326,287	0	G	T	27.3%	853.7 / 107.8	437	intergenic (+207/+200)	yddM/fdnI	transcriptional regulator/formate dehydrogenase nitrate‑inducible cytochrome b556(Fdn) subunit
Reads supporting (aligned to +/- strand): ref base G (206/98); new base T (24/90); total (235/202)
Fisher's exact test for biased strand distribution p-value = 5.45e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 439 total aligned reads displayed.

TGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATAT  >  DH1/2326139‑2326436

tGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGa                                                                                                                                                      >  3:1469249/1‑150 (MQ=33)

 gcgcTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGaa                                                                                                                                                     >  3:968648/1‑150 (MQ=33)

   gcTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACt                                                                                                                                                  <  7:44653/151‑1 (MQ=35)

   gcTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACt                                                                                                                                                  <  8:749612/151‑1 (MQ=35)

   gcTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGATATTAAAAGCCTCATAATGCGTAAACGACTGAAACTAAATGAACAAATCCTACGTAGACTAAAAAATAAAAATAAAAACTTTTACAGCGCCATTTGAAAcg                                                                                                                                                  >  6:1105968/1‑150 (MQ=2)

    cTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACtt                                                                                                                                                 >  1:671924/1‑150 (MQ=25)

     tACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACtg                                                                                                                                                 >  7:1230491/1‑150 (MQ=37)

      aCGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACtga                                                                                                                                                <  6:1258184/150‑1 (MQ=38)

      aCGCTTATCACGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACtga                                                                                                                                                <  8:343516/150‑1 (MQ=38)

       cGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACtgatg                                                                                                                                              <  2:861510/151‑1 (MQ=39)

         cTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTTAATCtgaaga                                                                                                                                             >  5:1661279/1‑150 (MQ=12)

            aTCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCC‑TTTACAGCGCCATTTGAAACTGATGACAAAc                                                                                                                                        <  6:290319/151‑1 (MQ=255)

               aGGCCTACACGTTCACTGCAATCTATTGGATTTGAATGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGTCGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGc                                                                                                                                      <  2:513424/151‑1 (MQ=255)

                ggCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATCAAAATCACGCCTTTTACAGCGCCATTTCAAACAGATGAGAACCGc                                                                                                                                      >  1:1253197/1‑150 (MQ=11)

                 gCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACTCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCaa                                                                                                                                    <  5:44672/151‑1 (MQ=255)

                  ccTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGATACTGATCACAAACGCAAc                                                                                                                                   >  5:309510/1‑151 (MQ=255)

                   cTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTTAAACttaaaaaaaaaaaaac                                                                                                                                   >  2:406153/1‑135 (MQ=11)

                     acacGTTCACTGCAATCTCTTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTTAAACtgatccaaaaataaaaatt                                                                                                                                >  1:323914/1‑136 (MQ=11)

                     acacGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACa                                                                                                                                  <  7:281274/149‑1 (MQ=255)

                       acGTTCACTTCAATCTATTGGATTTGAAGGGTTTTCATTCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATCAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGc                                                                                                                              <  1:87838/151‑1 (MQ=255)

                         gTTCACTGGCATGTATTGTCTTTGAAGGGTTTTCTTGCCGGATTCGGCGTTCACGCCGCCTCCGGCATGAACAAAGCCTACGTGGTCTTCAATATCAAAATGCCGCCTTTTACAGCGCCATTTGTAACTGATGACAAACGCAACATTGCCt                                                                                                                            <  2:996998/151‑1 (MQ=17)

                             aCTGCACTCTATTGGATTTGAAGGGTTTTCATGCCGCATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATg                                                                                                                        <  1:1571759/151‑1 (MQ=255)

                              atGCACTCTAATGGTCTTGAAGGGTTTTCTTGCCGTATCTGTCTTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATgc                                                                                                                       <  4:1517934/150‑1 (MQ=17)

                               tGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATgcg                                                                                                                      >  6:1410639/1‑151 (MQ=255)

                               tGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATgcg                                                                                                                      >  5:632604/1‑151 (MQ=255)

                                 cattCTTTTGGATTTGTAGCGTGTTGATGGCGTACTATGCGTTCACCCCGCTTCCGGCATGAACAAAGCCTACGTTGTATACAATATCAAAATGCCCCCTTATACAGCGCCATTTAAAACTGATGACAAACGCAACATTGCCTGATGCGCt                                                                                                                    <  1:1002105/148‑1 (MQ=17)

                                 cAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAATCTGATCACAAACGCAACATTGCCTGATGCGCt                                                                                                                    >  1:794145/1‑151 (MQ=255)

                                  aaTCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGCCAAAAGCAACATTGCCTGATGCGCt                                                                                                                    >  5:522676/1‑150 (MQ=255)

                                    tCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGATAcc                                                                                                                 >  2:721651/1‑150 (MQ=255)

                                       aatGGATTTGAATGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCTGCATGAACAAAGCCTACGTTGTATACAATATTAAAATGACGCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCtt                                                                                                              <  4:821309/149‑1 (MQ=18)

                                       aTTGGATTTGAAGGGTTTTCATGCCGGATATGTCTTTCACGCCGGATCCGGCATGAACAAAGCCTACGTTTTCTACAATATTAAAATGACGCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTAc                                                                                                                  <  8:1612721/147‑1 (MQ=18)

                                            aTTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCCCTACGCTTATc                                                                                                           >  4:476324/1‑149 (MQ=255)

                                                taaGGGTTTTGATGGCGGATAACGCTTTCACGCCTCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACTCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGcc                                                                                                      <  1:619230/149‑1 (MQ=21)

                                                gAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCt                                                                                                     >  7:1579854/1‑151 (MQ=255)

                                                    ggTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTATACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTacac                                                                                                 <  8:1390/151‑1 (MQ=255)

                                                        ttCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGTc                                                                                             >  5:333314/1‑151 (MQ=255)

                                                         tCATGCCGGATTATGCTTTCACGCCTCATCCGGCATGAACAAAGCCTACTTTGTCTACAATATTAAAATGTCTCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTc                                                                                             <  2:1585185/150‑1 (MQ=21)

                                                         tCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGCCAAACGCAACATTGCCTGATGCGCTACGCTTATCATGCCTACACGTTc                                                                                             >  5:993178/1‑150 (MQ=255)

                                                          cATTCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTATACAATATTAAAATGACGCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCa                                                                                            <  3:566597/150‑1 (MQ=255)

                                                          cATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCAc                                                                                           >  2:675501/1‑151 (MQ=255)

                                                           aTGGCGCATAAGTCGTTAACGCCTCATCGGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACt                                                                                          <  1:501411/151‑1 (MQ=25)

                                                           aTGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGACTAAACGTTCACt                                                                                          >  4:64879/1‑151 (MQ=255)

                                                               cGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCa                                                                                       >  3:1139559/1‑150 (MQ=255)

                                                                 gATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATc                                                                                    <  4:1256683/151‑1 (MQ=255)

                                                                  attacgCGTTCCCGCCTCATCCGTCATGAACAAATCCTACGTTTTTTACTATATGAAATTGTCGCCTTTTAGCGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATc                                                                                    <  2:145497/145‑1 (MQ=18)

                                                                  ataagtctatCACGACGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCt                                                                                   <  8:1015576/142‑1 (MQ=255)

                                                                   aaagtctttCACTCCTCTTCGGTCATGATAAAAGCCTTCTTTTTATTCAATAATAAAATGTCTCTTTTTAAAGCGCCATTTTTAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTa                                                                                  <  6:580613/144‑1 (MQ=2)

                                                                    ttggCGTTCAAGCCTCCTCCTGCATGAACAAAGCCTTCGTGGTCTACAATATGAAAATGACTCATTATACAGCGCCATTTCAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTa                                                                                  <  1:1133252/148‑1 (MQ=18)

                                                                       gCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATCAAAATGACGCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTgg                                                                              <  1:740157/151‑1 (MQ=255)

                                                                        cGTTCACGCCGCATCCTGCATGAACAAAGCCTACGTTGTCTACAATATCAAAATGACTCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTgg                                                                              <  6:197743/150‑1 (MQ=255)

                                                                         gTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGAt                                                                            <  7:1547907/151‑1 (MQ=255)

                                                                          ttctatcctcATCGGGCATGTACTAAGCCTACTTTTTATTCAATATTAAAATGACTCTTTTTAAAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTgg                                                                              <  4:99592/139‑1 (MQ=14)

                                                                           tCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGCCAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCGTACACGTTCACTGCAATCTATTGGAttt                                                                          >  4:1110158/1‑151 (MQ=255)

                                                                            cACGCCGCATCCGGGATGAACAAAGCCTACGTTGTCTACAATATCAAAATGACCCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGAttt                                                                          <  5:1237170/150‑1 (MQ=255)

                                                                            cACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTg                                                                         >  7:1403925/1‑151 (MQ=255)

                                                                            cACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGATTATCAGGACTACACGTTCACTGCAATCTATTGGAtttt                                                                         >  8:656726/1‑150 (MQ=255)

                                                                            cACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGAGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTg                                                                         >  2:806735/1‑151 (MQ=255)

                                                                            aagtcctcATCCGTCATGAACAAAGCCTACGTTTTCTACAATATGAAAATGACTCCTTTTACAGCGCCATTTGAAAGTTATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTg                                                                         <  8:394540/144‑1 (MQ=21)

                                                                              cgccgcATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTCCGCTTATCAGGCCTACACGTTCACTGCAAGCTATTGGATTTGa                                                                        >  5:872686/1‑150 (MQ=255)

                                                                                cctcctCCGGCATCTACAAAGCCTTCGTTTTAAACAATATAAAAATGTCTCTTTATACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAgg                                                                     <  4:27/146‑1 (MQ=11)

                                                                                   catgctgtaTGTACAAAGCCTACTTTTTATTCAATAATAAAATGTCTCCTTTTAAAGCGCCATTTTTAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAgg                                                                     <  8:1262961/140‑1 (MQ=11)

                                                                                      cggggaTGAACAAAGCCTACGTTGTATACAATATGAAAATGACGCCTTATACAGCGCCATTTTTAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTc                                                               <  7:1131476/146‑1 (MQ=18)

                                                                                      ccGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATTACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGCGtttt                                                                >  2:1293950/1‑150 (MQ=255)

                                                                                        ggCATGAACAAAGCCTACGTTTTCTATAATATTAAATTGACGGATTTTAAAGCGCCATTTCAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCAt                                                             <  8:1334583/151‑1 (MQ=17)

                                                                                        ggCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGCCCCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCAt                                                             <  5:1178271/151‑1 (MQ=255)

                                                                                             gaagaaAGCCTTCGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCgg                                                        <  3:517488/151‑1 (MQ=255)

                                                                                             gAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGACTGATGCGCTACCATTATCAGGACTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATgaagt                                                        >  2:1117415/1‑147 (MQ=255)

                                                                                                 aaaGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATa                                                     >  3:1562166/1‑150 (MQ=255)

                                                                                                    gCCTACTTTGTCTACAATATTAAAATGACGCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGc                                                 <  3:731237/151‑1 (MQ=255)

                                                                                                       tACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGtt                                              >  1:1412853/1‑151 (MQ=255)

                                                                                                         cGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTc                                             >  5:779896/1‑150 (MQ=255)

                                                                                                            tGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCAcgc                                         >  5:1439042/1‑151 (MQ=255)

                                                                                                             gTCTACAATATCAAAATGACCCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCAcgc                                         <  5:723763/150‑1 (MQ=255)

                                                                                                              tCTACAATATGAAAATGACGCCTTATACAGCGCCATTTGAAACTGATGACAAACCCAACATTGCCTGATCCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCAcgcc                                        <  7:626316/150‑1 (MQ=255)

                                                                                                                tACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCAcgccgc                                      >  3:8086/1‑150 (MQ=255)

                                                                                                                tACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCAcgccgc                                      >  7:757742/1‑150 (MQ=255)

                                                                                                                 aCATTATGAAATTTATTCCTTTTTAAGCGCCATTTTTAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCAt                                    <  6:428281/151‑1 (MQ=11)

                                                                                                                  cAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATc                                   <  7:662536/151‑1 (MQ=255)

                                                                                                                   attatTAAAATGACGCCTTTTTCAGGTTCTTTTCAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATcc                                  <  6:843917/149‑1 (MQ=14)

                                                                                                                   aaTATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATcc                                  >  4:17713/1‑151 (MQ=255)

                                                                                                                    atatGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGAGTTCACGACGCATCCg                                 >  3:1311562/1‑151 (MQ=255)

                                                                                                                      atGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCgg                                <  7:1010471/150‑1 (MQ=255)

                                                                                                                         aaaaTGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATg                            >  8:1468931/1‑151 (MQ=255)

                                                                                                                           aaTGACTCTTTTTAAAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGaa                          <  4:1349862/151‑1 (MQ=9)

                                                                                                                           aaTGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGa                           >  3:245116/1‑150 (MQ=255)

                                                                                                                           aaTGACGCCTTATACAGCGCCATTTCAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGaa                          <  5:1349404/151‑1 (MQ=255)

                                                                                                                            aTGACTCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAAc                         <  8:1532801/151‑1 (MQ=255)

                                                                                                                              gACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACa                        >  3:44482/1‑150 (MQ=255)

                                                                                                                               aatccTTTTAAAGCGACATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGGGTTCACGCCGCATCCGGCATGAACaaa                      <  8:12082/148‑1 (MQ=255)

                                                                                                                                cGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACaa                       >  7:212867/1‑149 (MQ=255)

                                                                                                                                      ttACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAGCAAAGCCTACg               >  3:31185/1‑151 (MQ=37)

                                                                                                                                      ttACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACg               >  8:1317742/1‑151 (MQ=37)

                                                                                                                                       tACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGt              >  8:846430/1‑151 (MQ=35)

                                                                                                                                           gcgcCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTc          >  6:1563346/1‑151 (MQ=33)

                                                                                                                                           gcgcCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTc          >  8:444371/1‑151 (MQ=33)

                                                                                                                                              ccATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTAc       >  6:93933/1‑151 (MQ=33)

                                                                                                                                                aTTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACaa     <  1:1631022/151‑1 (MQ=14)

                                                                                                                                                 tttGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAat    >  8:55956/1‑151 (MQ=33)

                                                                                                                                                   tGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAatat  >  7:893347/1‑151 (MQ=31)

                                                                                                                                                   tGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAatat  >  2:263936/1‑151 (MQ=31)

TGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATAT  >  DH1/2326139‑2326436

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`