Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH13,447,4030AC29.6% 538.5 / 90.5 325G346G (GGT→GGG) malZalpha‑glycosidase
Reads supporting (aligned to +/- strand):  ref base A (76/152);  new base C (95/1);  total (171/154)
Fisher's exact test for biased strand distribution p-value = 6.96e-33
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 340 total aligned reads displayed.

GCCACGATAGTTCATGGCGGCATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTCGGCAAGCTGGC  >  DH1/3447253‑3447552
                                                                                                                                                      |                                                                                                                                                     
 ccACGATAGTTCATGGCGGCATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCAc                                                                                                                                                      <  8:1200915/151‑1 (MQ=255)
 ccACGATAGTTCATGGCGGCATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCAc                                                                                                                                                      >  7:681646/1‑151 (MQ=255)
  cACGATAGTTCATGGCGGCATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCcccccc                                                                                                                                                     >  7:1035954/1‑151 (MQ=255)
   aCGATAGTTCATGGCGGCATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGcccccccc                                                                                                                                                    >  2:778582/1‑151 (MQ=255)
   aCGATAGTTCATGGCGGCATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCAccc                                                                                                                                                    <  7:18959/151‑1 (MQ=255)
      aTAGTTCATGGCGGCATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCGCCCCCCGcc                                                                                                                                                 >  5:237789/1‑151 (MQ=255)
        aGTTCATGGCGGCATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCccccccccccgc                                                                                                                                               >  5:338264/1‑149 (MQ=255)
        aGTTCATGGCGGCATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCt                                                                                                                                                <  1:878042/150‑1 (MQ=255)
               ggcggcATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCCCCCCCCAGc                                                                                                                                        >  8:796899/1‑151 (MQ=255)
                gcggcATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCCGCCCCCAGc                                                                                                                                        >  2:1460222/1‑150 (MQ=255)
                  ggcATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCata                                                                                                                                     <  1:567167/151‑1 (MQ=255)
                  ggcATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCata                                                                                                                                     <  6:233868/151‑1 (MQ=255)
                   gcATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAACTGTGCGCCGACAATATACGCTTCCGGCTGGGTTGCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCatat                                                                                                                                    <  3:37271/151‑1 (MQ=255)
                    cATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGcccccacccccacccccacgata                                                                                                                                     >  2:243207/1‑144 (MQ=255)
                     aTCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGc                                                                                                                                  >  3:1469029/1‑151 (MQ=255)
                      tCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGcc                                                                                                                                 >  1:765926/1‑150 (MQ=255)
                       cTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCCTCCCCCAGCATATGcac                                                                                                                                >  4:1199881/1‑151 (MQ=255)
                        ttCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCACCGTGCTGCATATTATTGCGCGCCCCCCCCGCCGCCCCCCGCCTATGcccc                                                                                                                               >  8:409258/1‑151 (MQ=255)
                          ccACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGcacca                                                                                                                              <  5:905181/150‑1 (MQ=255)
                          ccACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGCCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGcaccac                                                                                                                             >  1:1243360/1‑151 (MQ=255)
                           cacaTCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCCCCAgccccccccaaaggcccac                                                                                                                             >  4:830658/1‑136 (MQ=255)
                            acaTCGGCCTGTAACCATTGCCGTGAATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACAt                                                                                                                           <  7:272100/151‑1 (MQ=255)
                             caTCGGCCTGTAACGATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATc                                                                                                                          <  4:1054443/151‑1 (MQ=255)
                                   ccTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATccagccg                                                                                                                    <  5:594935/151‑1 (MQ=255)
                                      gTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTTCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATccagccgcca                                                                                                                 <  8:1001220/151‑1 (MQ=255)
                                         aCCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATccagccgccagcc                                                                                                              <  5:1646506/151‑1 (MQ=255)
                                               gCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCat                                                                                                        <  3:794294/151‑1 (MQ=255)
                                               gCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCCCCTCCCCCACCATATGCACCACATCCACCCGCCAGCCCTCCa                                                                                                         >  4:639377/1‑150 (MQ=255)
                                                 cGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCatat                                                                                                      <  2:97532/151‑1 (MQ=255)
                                                 cGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCatat                                                                                                      <  6:1424179/151‑1 (MQ=255)
                                                        cGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACg                                                                                                <  1:656081/150‑1 (MQ=255)
                                                           cAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCGCCCCCCGCATATGCCCCACATCCAGCCGCCCGCCCGCCATATTACACGgcgc                                                                                            >  6:851642/1‑151 (MQ=255)
                                                              aaTGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCCGCATATGCACCCCCTCCAGCCGCCAGCCCTCCATATTCCCCGGCGCttt                                                                                         >  8:1295284/1‑151 (MQ=255)
                                                                tGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCGCCCCCCCCCGCCCCCCGCATATTCACCCCATCCACCCGCCCCCCGTGCATATTCCCCGGCGCGTTcc                                                                                       >  3:1343881/1‑150 (MQ=255)
                                                                 gTTCGCCTACAATATACGCTTGCGGCGGGGGTTCTGTCGCCGCTTCGGTGAGCCCGGCAACGGGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCa                                                                                       <  4:290389/150‑1 (MQ=255)
                                                                 gTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCa                                                                                       <  6:337491/150‑1 (MQ=255)
                                                                 gTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCa                                                                                       <  2:425957/150‑1 (MQ=255)
                                                                  ttCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCACCCCCTCCCCCCCCAAATTCCCCCCAACCACACCCCAGCCGTCCAAATTACaacccccattgaac                                                                                     >  3:234748/1‑138 (MQ=255)
                                                                    cGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCa                                                                                   <  7:1488923/151‑1 (MQ=255)
                                                                     cccGACAATATACGCTGCCGGCTGGGGTGCCTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCCGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAg                                                                                  <  4:1473044/150‑1 (MQ=255)
                                                                      ccGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGt                                                                                 <  1:454847/151‑1 (MQ=255)
                                                                       cGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCCTCCCCCAGCATATGCACCCCATCCAGCCGCCCGCCGTCCATATTACACGGCGCGTTCCGCCAGTg                                                                                >  1:354610/1‑151 (MQ=255)
                                                                       cGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCCTCCCCCAGCAAAAGCACCACATCCACCCGCCAGCCGTCCATATTCCACCGCGCTTTCAGCCAGTg                                                                                >  2:1350379/1‑151 (MQ=255)
                                                                       cGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTg                                                                                <  8:34563/151‑1 (MQ=255)
                                                                        gACAATATACGCTTCCGGCTGGGTTACTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCCGCAAATACACCCCAACCAGCCGCCACCCGTCCATATTGCACGGCGCTTTAACCCACTgt                                                                               >  4:583104/1‑150 (MQ=255)
                                                                         aCAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCCCCCCCCCCCCCAGATTCCCCACAACCCGCCGCCCGCCGGCCCAATTCCACCGCGGGTTGAGCCacgggg                                                                              >  5:520158/1‑146 (MQ=255)
                                                                            atatACGCTTCCGGATGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCAGATTATTGCGCGCCCCACCCGCATCCCCCCCACTATTCCCCACAACCCACCACCACCCCTCAAAATTCCACATCGCTTTAAGCCAGTGGCggc                                                                           >  4:914542/1‑150 (MQ=255)
                                                                              ataCGCTTCCGGCTGGGTTACTTTCGCCGCTTCGGTGATCCCGGCAACTTGCTGCATATTATTGCGCGCCCCACCCCCCTCCCCCACCAAATGCACCCCAGCCACCCGCCAGCCGTCGAAATAAAACGGCGCGTTCAGCCAGGGGccgcc                                                                          >  8:108522/1‑146 (MQ=255)
                                                                                ccgcgTCCGGCTGGGTTGCTTTCGCCGGTTCGGTGATCCCGGCAACGTTCTGCATATTATTGCGCGCCCATCCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGTCGGACAAt                                                                       <  6:1645908/150‑1 (MQ=255)
                                                                                aCGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCACCCCCAGCATAAGCCCCACAAACAGCCGCCCACCCTCCAAAATACACCGCGCATAAAGCCAGTAGCCGACaa                                                                        >  6:506703/1‑150 (MQ=255)
                                                                                  gCTTCCGCCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCCGCCCCCACCATATGCACCACATCCACCCCCCAGCCGGCCAAATTCCACCGCCCTTTCAGCCAGTGGCCGCCAATAc                                                                     >  5:1509003/1‑151 (MQ=255)
                                                                                     tCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCACCGGCTCCCCCAGCATATGCCCCCCATCCCGCCGGCCGCCCTCCCTATTAACCCGCGCTTTAAGGCAGTGGCGGGCCATACTgg                                                                  >  7:1319035/1‑150 (MQ=255)
                                                                                       cGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCCCCGCCCCCCGCATATGCACCACAACCAGCCCCCAGCCCTCCATATTACACCGCCCGTTCAGCCACTTGCGGCCAAAACTGTCt                                                                >  5:1145947/1‑151 (MQ=255)
                                                                                        ggCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCtt                                                               >  2:1546814/1‑151 (MQ=255)
                                                                                        ggCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCAACACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCtt                                                               >  8:179074/1‑151 (MQ=255)
                                                                                           tGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCCAGCCCCCCCAAATGCACCCCATCCAGCCCCCCGCCGGCCATATTACACCGCGCGTTCAGCCAGTGGCGGCCAATACGGGCTTccc                                                            >  1:1094485/1‑151 (MQ=255)
                                                                                            gggTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTcc                                                             <  5:1303919/149‑1 (MQ=255)
                                                                                               tttctttcGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCCGCCCCCAGCAAATGCCCCCCAAACCGCGCCCCGCCGGACAAATTACACCGCGCTTTCAGCCAGGGGCGGGCAATAAAGTGTTACCcgcg                                                        >  2:1120773/1‑151 (MQ=255)
                                                                                               tttctttcGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCCCCGGCCCCCCCCAATTGACCACAACCCCCCCCCCGCCCGCCAAATTACACCGCGCGTTAAGCCACTGGCGGGCAAAAAGGTCTTGCCcgcg                                                        >  6:924506/1‑151 (MQ=255)
                                                                                                    ttcGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCGCCCCCCGCCTGCCCCCGCAAATGCACCCCATCCCGCCCCCAGCCGTCCCTATTCCACGGCGCTTTCAGCCCGTGGGGGCCAATACGGTCTTCCCCGCGaaaa                                                    >  2:619618/1‑150 (MQ=255)
                                                                                                    ttcGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGAt                                                      <  2:566638/148‑1 (MQ=255)
                                                                                                    ttcGCCGCTTCGGTGATCCCGGCAACGTGCGGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATaa                                                    <  5:125485/150‑1 (MQ=255)
                                                                                                      cgccgcTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCGACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAAtt                                                 <  1:1324631/151‑1 (MQ=255)
                                                                                                      cgccgcTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAAtt                                                 <  5:485130/151‑1 (MQ=255)
                                                                                                       gccgcTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCCTCCATATTCCACGGCGCGTTCAGCCAGTGGCGGCCAATACTGTCTTCCCCGCGATAAAttt                                                >  1:1509334/1‑151 (MQ=255)
                                                                                                        ccgcTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCGCCCCCCGCCTCCCCCACCAAATGCACCCCCAACAGCCCCCCCCCCAACATTTTACAACGCCCGTTCATCCAGATGCCGCCAAATCTGGCTTCCCCCCGATaaaaat                                                >  1:1396334/1‑147 (MQ=255)
                                                                                                          gcTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCCGCATATGCACCACATCCAGCCGCCCGCCGTCCAAATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTGTTCCCCGCGCTAAATttcat                                             >  6:1520263/1‑151 (MQ=255)
                                                                                                          gcTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATttcat                                             <  4:374275/151‑1 (MQ=255)
                                                                                                             tCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATttcatt                                            <  3:603892/149‑1 (MQ=255)
                                                                                                             tCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTACACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATttcattc                                           >  2:1255979/1‑150 (MQ=255)
                                                                                                              cGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATttcattca                                          <  6:1217168/150‑1 (MQ=255)
                                                                                                                  gATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGa                                     <  1:1587015/151‑1 (MQ=255)
                                                                                                                  gATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGa                                     <  8:584232/151‑1 (MQ=255)
                                                                                                                    tCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACCGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTAATTCACCACACt                                   >  2:397427/1‑151 (MQ=255)
                                                                                                                     cccGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACtt                                  <  7:498985/151‑1 (MQ=255)
                                                                                                                     cccGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACt                                   <  5:268203/150‑1 (MQ=255)
                                                                                                                     cccGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACt                                   <  7:379887/150‑1 (MQ=255)
                                                                                                                      ccGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACttt                                 <  1:513313/151‑1 (MQ=255)
                                                                                                                      ccGGCAACGTGCTGCATATTATTGCGCGCCCCACCCCCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGGCCATAATCCACCGCGCGTTCAGCCAGTGGCGGGCCATACTGTGGTCCCCCCGATAAAATTAAATCACCAGCCttt                                 >  4:491725/1‑151 (MQ=255)
                                                                                                                       cGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTc                                <  8:1552445/151‑1 (MQ=255)
                                                                                                                        ggCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTcc                               <  6:688620/151‑1 (MQ=255)
                                                                                                                        ggCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTcc                               <  7:1570103/151‑1 (MQ=255)
                                                                                                                         gCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGCCAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCg                              >  3:656973/1‑151 (MQ=255)
                                                                                                                         ccAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATAAGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCg                              <  4:1369591/150‑1 (MQ=255)
                                                                                                                              gTGCTGCATATTATTGCGCGCCCCCCCCGCCTCCCCCCGCATATGGACCACATCCAGCCGCCAGCCGTCCATATTACACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTAATTCACCACACTTTCCGACTg                          >  3:432994/1‑150 (MQ=255)
                                                                                                                                  tgcATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTACACCGCGCTTTCAGCCAGTGGCGGCCAATACTGTCTTCCCCGCGATAAATTTAATTGACCAGACATTGCGACAGATAAt                     >  8:546423/1‑151 (MQ=255)
                                                                                                                                   gcATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATc                    <  5:817448/151‑1 (MQ=255)
                                                                                                                                    cATATTATTGCGCGCCCCCCCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTAACCAGACTTTCCGACTGATAATcc                   >  5:675956/1‑151 (MQ=255)
                                                                                                                                    cATATTATTGCGCGACCCACCGGCCTCGTCCAGCATATGGACCACATCCAGCGGCCAGCAGTAGATATTCCACGGGGCTTTCAGCCAGTGGAGGACAATACTGTCTTCCACGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATcc                   <  2:912760/151‑1 (MQ=255)
                                                                                                                                     atatTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCCCATACAGCCGCCAGCCGTCCATATTCCACGGCGCTTTAAGCCAGTGGCGGCCAATACTGTCTTACCCGCGATAAATTTGATTAACCAGACATTCCGACTGATAATcc                   >  4:1291795/1‑150 (MQ=255)
                                                                                                                                     atatTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAAATTCATTCACCAGACTTTCCGACTGATAATCCa                  <  6:1031317/151‑1 (MQ=255)
                                                                                                                                        ttattGCGCGCCCCCCCCGCCTCCCCCCCCATATGCACCACATACAGCCGCCAGCCGTCCATATTCCACCGCGCGTTCAGCCAGTGGCGGACAAAACTGTCTTGCCCGCGATAAATTTCATTCACCAGACTTTCCGACAGATAAACCACCt               >  3:916601/1‑151 (MQ=255)
                                                                                                                                         tattGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCt               <  3:1105938/150‑1 (MQ=255)
                                                                                                                                           ttGCGCGCCCCCCCCGCCTCCCCCCGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGGGTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTGACAAGACTTTCCGACTGATAATCCAGCTTCg            >  5:143270/1‑151 (MQ=255)
                                                                                                                                            tGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTCg            <  1:712551/150‑1 (MQ=255)
                                                                                                                                              cgcgcCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGGCCATATTACACGGCGCATTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTAATTCCCCAGACTTTCCGACTGATAATACAGCGTCGGc          >  4:856467/1‑150 (MQ=255)
                                                                                                                                              cgcgcCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCCCCAGCCGTCCATATTACACGGCGCTTTCACCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTGATTCACCAGACTTTACGACTGATAATCCACCTTCGGc          >  1:1191172/1‑150 (MQ=255)
                                                                                                                                                 gcCCCACCCGCCGCCCCCCGCATTTGCACCACATCCCGCCGCCAGCCCTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATAATGTCTTCCCCGCGATAAATTTCATTGACCATCCTTTCCGACGGATTATCCAGCTTCGGCAAGc      >  4:99842/1‑151 (MQ=255)
                                                                                                                                                    cgaCCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCAGGGCGCTTTCAGCCATTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACGAGACTTTCCGACTGATAATGCAGCTTCGGCAAGCTgg   <  8:13637/149‑1 (MQ=255)
                                                                                                                                                    ccACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTCGGCAAGCTgg   >  7:591539/1‑151 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
GCCACGATAGTTCATGGCGGCATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTCGGCAAGCTGGC  >  DH1/3447253‑3447552

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑